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Electrochemical Detection of Vibrio cholerae by Amine Functionalized Biocompatible Gadolinium Oxide Nanoparticles.
Electrochemical Detection of Vibrio cholerae by Amine Functionalized Biocompatible Gadolinium Oxide Nanoparticles.
- Source :
- Micromachines; May2023, Vol. 14 Issue 5, p995, 13p
- Publication Year :
- 2023
-
Abstract
- Herein, we report the biocompatible amine-functionalized gadolinium oxide nanoparticles (Gd<subscript>2</subscript>O<subscript>3</subscript> NPs) for the possibility of electrochemical detection of Vibrio cholerae (Vc) cells. The microwave irradiation process is applied to synthesize Gd<subscript>2</subscript>O<subscript>3</subscript> NPs. The amine (NH<subscript>2</subscript>) functionalization is carried out via overnight stirring with 3(Aminopropyl)triethoxysilane (APTES) at 55 °C. The size of NPs amine functionalized APETS@Gd<subscript>2</subscript>O<subscript>3</subscript> NPs are determined by transmission electron microscopy (TEM). APETS@Gd<subscript>2</subscript>O<subscript>3</subscript> NPs are further electrophoretically deposited onto indium tin oxide (ITO) coated glass substrate to obtain working electrode surface. The monoclonal antibodies (anti-CT) specific to cholera toxin associated to Vc cells are covalently immobilized onto the above electrodes using EDC-NHS chemistry and further BSA is added to obtain the BSA/anti-CT/APETS@Gd<subscript>2</subscript>O<subscript>3</subscript>/ITO immunoelectrode. Further, this immunoelectrode shows the response for cells in CFU range from 3.125 × 10<superscript>6</superscript> to 30 × 10<superscript>6</superscript> and is very selective with sensitivity and LOD 5.07 mA CFUs mL cm<superscript>−2</superscript> and 0.9375 × 10<superscript>6</superscript> CFU respectively. To establish a future potential for APTES@Gd<subscript>2</subscript>O<subscript>3</subscript> NPs in field of biomedical applications and cytosensing, the effect of APTES@Gd<subscript>2</subscript>O<subscript>3</subscript> NPs on mammalian cells is also observed using in vitro cytotoxicity assay and cell cycle analysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2072666X
- Volume :
- 14
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Micromachines
- Publication Type :
- Academic Journal
- Accession number :
- 163969544
- Full Text :
- https://doi.org/10.3390/mi14050995