1. Phylogenetic analysis of Histoplasma capsulatum var duboisii in baboons from archived formalin-fixed, paraffin embedded tissues.
- Author
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Hensel M, Hoffmann AR, Gonzales M, Owston MA, and Dick EJ Jr
- Subjects
- Africa epidemiology, Animals, DNA, Fungal genetics, DNA, Ribosomal Spacer genetics, Formaldehyde, Genes, Fungal genetics, Histoplasma genetics, Histoplasmosis epidemiology, Histoplasmosis microbiology, Molecular Epidemiology, Paraffin Embedding veterinary, Primate Diseases epidemiology, Retrospective Studies, Sequence Analysis, DNA, Texas epidemiology, Histoplasma classification, Histoplasma isolation & purification, Histoplasmosis veterinary, Papio microbiology, Phylogeny, Primate Diseases microbiology
- Abstract
Histoplasma capsulatum var. duboisii (Hcd) infections have been well documented to cause chronic granulomatous disease, mainly involving the skin of baboons and humans in African countries primarily. This retrospective study classified the subspecies of Histoplasma and developed a phylogenetic tree utilizing DNA sequences extracted from formalin-fixed, paraffin embedded (FFPE) tissues from 9 baboons from a research colony in Texas histologically diagnosed with Hcd. Based on sequence analysis of ITS-2, Tub-1, and ARF, Hcd isolated from the archived samples closely aligns with the African clade and has 88% sequence homology with a sample isolated from an individual in Senegal.
- Published
- 2019
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