23 results on '"Na Kyoung Lee"'
Search Results
2. Pharmacological inhibition of mTOR attenuates replicative cell senescence and improves cellular function via regulating the STAT3-PIM1 axis in human cardiac progenitor cells
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Ji Hye Park, Na Kyoung Lee, Hye Ji Lim, Seung taek Ji, Yeon-Ju Kim, Woong Bi Jang, Da Yeon Kim, Songhwa Kang, Jisoo Yun, Jong seong Ha, Hyungtae Kim, Dongjun Lee, Sang Hong Baek, and Sang-Mo Kwon
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Medicine ,Biochemistry ,QD415-436 - Abstract
Abstract The mammalian target of rapamycin (mTOR) signaling pathway efficiently regulates the energy state of cells and maintains tissue homeostasis. Dysregulation of the mTOR pathway has been implicated in several human diseases. Rapamycin is a specific inhibitor of mTOR and pharmacological inhibition of mTOR with rapamycin promote cardiac cell generation from the differentiation of mouse and human embryonic stem cells. These studies strongly implicate a role of sustained mTOR activity in the differentiating functions of embryonic stem cells; however, they do not directly address the required effect for sustained mTOR activity in human cardiac progenitor cells. In the present study, we evaluated the effect of mTOR inhibition by rapamycin on the cellular function of human cardiac progenitor cells and discovered that treatment with rapamycin markedly attenuated replicative cell senescence in human cardiac progenitor cells (hCPCs) and promoted their cellular functions. Furthermore, rapamycin not only inhibited mTOR signaling but also influenced signaling pathways, including STAT3 and PIM1, in hCPCs. Therefore, these data reveal a crucial function for rapamycin in senescent hCPCs and provide clinical strategies based on chronic mTOR activity.
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- 2020
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3. Investigation of Brassica juncea, Forsythia suspensa, and Inula britannica: phytochemical properties, antiviral effects, and safety
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Won-Young Bae, Hyeong-Yeop Kim, Kyoung-Sook Choi, Kyung Hoon Chang, Young-Ho Hong, Jongsu Eun, Na-Kyoung Lee, and Hyun-Dong Paik
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Medicinal herbs ,Phytochemical properties ,Antiviral effect ,Human safety ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background General antiviral agents such as oseltamivir are associated with certain adverse effects and the emergence of resistance. This study investigated the phytochemical properties, antiviral activities, and safety of three herbs used in traditional Korean medicine. Methods Extracts of three medicinal herbs (Brassica juncea, Forsythia suspensa, and Inula britannica) were prepared using ethanol or water. The total phenolic, flavonoid, and saponin content, condensed tannin content, and reducing sugar content of the herb extracts were determined via phytochemical screening. Tandem mass analysis was performed using an ultra-performance liquid chromatography (UPLC)-electrospray ionization (ESI)-Q/Orbitrap instrument. Virus titrations were determined via tissue culture infective dose (TCID50) and cytotoxicity assays. Hemolysis and hepatotoxicity were measured to determine safety. Results Among the three medicinal herbs, F. suspensa showed the highest concentration of phenolic compounds, flavonoids, and saponins. The number of phytochemical compounds detected via tandem mass analysis of B. juncea, F. suspensa, and I. britannica was 5 (including sinigrin, m/z [M-H] = 358.02), 14 (including forsythoside A, m/z [M-H] = 623.19), and 18 (including chlorogenic acid, m/z [M-H] = 353.20), respectively. The antiviral effects of the B. juncea extracts (ethanol and water) and I. britannica extract (ethanol) were further investigated. The ethanol extract of B. juncea showed a 3 Log TCID50/25 μL virus titration reduction and the water extract showed a selectivity index of 13.668 against infected influenza H1N1 virus A/NWS/33. The B. juncea extracts did not show hemolysis activities and hepatotoxicity (
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- 2019
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4. Fermentation of red ginseng extract by the probiotic Lactobacillus plantarum KCCM 11613P: ginsenoside conversion and antioxidant effects
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Jieun Jung, Hye Ji Jang, Su Jin Eom, Nam Soon Choi, Na-Kyoung Lee, and Hyun-Dong Paik
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Botany ,QK1-989 - Abstract
Background: Ginsenosides, which are bioactive components in ginseng, can be converted to smaller compounds for improvement of their pharmacological activities. The conversion methods include heating; acid, alkali, and enzymatic treatment; and microbial conversion. The aim of this study was to determine the bioconversion of ginsenosides in fermented red ginseng extract (FRGE). Methods: Red ginseng extract (RGE) was fermented using Lactobacillus plantarum KCCM 11613P. This study investigated the ginsenosides and their antioxidant capacity in FRGE using diverse methods. Results: Properties of RGE were changed upon fermentation. Fermentation reduced the pH value, but increased the titratable acidity and viable cell counts of lactic acid bacteria. L. plantarum KCCM 11613P converted ginsenosides Rb2 and Rb3 to ginsenoside Rd in RGE. Fermentation also enhanced the antioxidant effects of RGE. FRGE reduced 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and reducing power; however, it improved the inhibition of β-carotene and linoleic acid oxidation and the lipid peroxidation. This suggested that the fermentation of RGE is effective for producing ginsenoside Rd as precursor of ginsenoside compound K and inhibition of lipid oxidation. Conclusion: This study showed that RGE fermented by L. plantarum KCCM 11613P may contribute to the development of functional food materials. Keywords: antioxidant effect, bioconversion, ginsenoside, Lactobacillus plantarum KCCM 11613P, red ginseng extract
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- 2019
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5. Inula britannica Inhibits Adipogenesis of 3T3-L1 Preadipocytes via Modulation of Mitotic Clonal Expansion Involving ERK 1/2 and Akt Signaling Pathways
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Hyung-Seok Yu, Won-Ju Kim, Won-Young Bae, Na-Kyoung Lee, and Hyun-Dong Paik
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Inula britannica ,anti-obesity ,adipogenesis ,lipogenesis ,mitotic clonal expansion ,ERK 1/2 signaling pathways ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The flower of Inula britannica contains various phenolic compounds with prophylactic properties. This study aimed to determine the anti-adipogenic effect of an I. britannica flower aqueous extract (IAE) and its underlying mechanisms in the 3T3-L1 preadipocytes and to identify the phenolic compounds in the extract. Treatment with IAE inhibited the adipogenesis of 3T3-L1 preadipocytes by showing a dose-dependently suppressed intracellular lipid accumulation and significantly mitigated expression levels of lipogenesis- and adipogenesis-associated biomarkers including transcription factors. IAE exerted an anti-adipogenic effect through the modulation of the early phases of adipogenesis including mitotic clonal expansion (MCE). Treatment with IAE inhibited MCE by arresting the cell cycle at the G0/G1 phase and suppressing the activation of MCE-related transcription factors. Furthermore, IAE inhibited adipogenesis by regulating the extracellular signal-regulated kinase 1/2 and Akt signaling pathways. Protocatechuic acid, chlorogenic acid, kaempferol-3-O-glucoside, and 6-methoxyluteolin, which are reported to exhibit anti-adipogenic properties, were detected in IAE. Therefore, modulation of early phases of adipogenesis, especially MCE, is a key mechanism underlying the anti-adipogenic activity of IAE. In summary, the anti-obesity effects of IAE can be attributed to its phenolic compounds, and hence, IAE can be used for the development of anti-obesity products.
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- 2020
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6. Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods
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Min-Jeong Cheon, Sung-Min Lim, Na-Kyoung Lee, and Hyun-Dong Paik
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probiotics ,kimchi ,lactobacillus buchneri ,gut-brain-axis ,neuroprotective effect ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The purpose of this study was to evaluate the probiotic characteristics and neuroprotective effects of bacteria isolated from Korean fermented foods. Three bacterial strains (Lactobacillus fermentum KU200060, Lactobacillus delbrueckii KU200171, and Lactobacillus buchneri KU200793) showed potential probiotic properties, such as high tolerance against artificial gastric juice and bile salts, sensitivity to antibiotics, nonproduction of carcinogenic enzymes, and high adhesion to intestinal cells. Heat-killed L. fermentum KU200060 and L. buchneri KU200793 showed higher antioxidant activity than heat-killed L. delbrueckii KU200171. The conditioned medium (CM) was used to evaluate the reaction between HT-29 cells and each heat-killed strain. All CMs protected SH-SY5Y cells from 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. The expression of brain-derived neurotropic factor (BDNF) mRNA in HT-29 cells treated with CM containing heat-killed L. buchneri KU200793 was the highest. The CM significantly reduced the Bax/Bcl-2 ratio and increased BDNF mRNA expression in SH-SY5Y cells treated with MPP+. These results indicate that L. buchneri KU200793 can be used as a prophylactic functional food, having probiotic potential and neuroprotective effects.
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- 2020
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7. The Anti-Cancer Potential of Heat-Killed Lactobacillus brevis KU15176 upon AGS Cell Lines through Intrinsic Apoptosis Pathway
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Chang-Hoon Hwang, Na-Kyoung Lee, and Hyun-Dong Paik
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Inorganic Chemistry ,Organic Chemistry ,Lactobacillus brevis ,probiotics ,apoptosis ,cancer therapy ,caspases ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
Recent research has focused on the anti-cancer properties of Lactobacillus strains isolated from fermented foods. Their anti-cancer effects are caused by the apoptosis induction in cancer cells. However, sepsis, which can occur when cancer patients consume living organisms, can cause serious conditions in patients with reduced immunity because of cancer. Therefore, this study was conducted using heat-killed Lactobacillus brevis KU15176 (KU15176). To determine the relationship between inflammation and cancer, the anti-inflammatory effect of KU15176 was evaluated using a nitric oxide (NO) assay. Then, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was conducted to select cancer cells that showed the anti-proliferative effect of KU15176. Next, reverse transcription-polymerase chain reaction (RT-PCR), 4′,6-diamidino-2-phenylindole (DAPI) staining, flow cytometry, and caspase colorimetric assay were performed. As a result, it was confirmed that KU15176 could cause the increasing expression of apoptosis-related genes (Bax, caspase-3, and caspase-9), DNA breakage, effective apoptosis rate, and increased caspase activity in the human stomach adenocarcinoma (AGS) gastric cancer cell line. In conclusion, these results suggest a potential prophylactic effect of KU15176 against cancer.
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- 2022
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8. Antimicrobial and Antibiofilm Effect of ε-Polylysine against Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli in Tryptic Soy Broth and Chicken Juice
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Yeong Jin Park, Do-Un Lee, Na-Kyoung Lee, Suk-Chae Jung, Hwan Hee Yu, Hyun-Dong Paik, Dae-Hee Lee, and Jung-Hee Park
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Salmonella ,Health (social science) ,Salmonella enteritidis ,TP1-1185 ,Plant Science ,medicine.disease_cause ,Health Professions (miscellaneous) ,Microbiology ,Tryptic soy broth ,chemistry.chemical_compound ,Listeria monocytogenes ,antimicrobial effect ,medicine ,Food science ,Escherichia coli ,ε-polylysine ,biology ,antibiofilm ,Chemical technology ,Biofilm ,biology.organism_classification ,Antimicrobial ,chemistry ,Salmonella Enteritidis ,Listeria ,Food Science - Abstract
ε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031–1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to ε-PL depended on the time and concentration, and 1/2 × MIC of ε-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (Salmonella, Listeria, E. coli, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2 × MIC of ε-PL, respectively. Additionally, when observed under a CLSM, ε-PL was found to induce biofilm destruction and bacterial cytotoxicity. These results demonstrated that ε-PL has the potential to be used as an antibiotic and antibiofilm material for chicken meat processing.
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- 2021
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9. Investigation of Brassica juncea, Forsythia suspensa, and Inula britannica: phytochemical properties, antiviral effects, and safety
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Young-Ho Hong, Hyeong-Yeop Kim, Hyun-Dong Paik, Kyung Hoon Chang, Na-Kyoung Lee, Jongsu Eun, Won-Young Bae, and Kyoung-Sook Choi
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Human safety ,food.ingredient ,Phytochemicals ,Flavonoid ,Saponin ,Antiviral Agents ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Antiviral effect ,Influenza A Virus, H1N1 Subtype ,0302 clinical medicine ,food ,Chlorogenic acid ,Influenza, Human ,Humans ,Medicinal herbs ,Medicinal plants ,Forsythia ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Forsythia suspensa ,biology ,Traditional medicine ,Plant Extracts ,Chemistry ,food and beverages ,General Medicine ,lcsh:Other systems of medicine ,biology.organism_classification ,lcsh:RZ201-999 ,Complementary and alternative medicine ,Inula britannica ,Phytochemical ,Phytochemical properties ,030220 oncology & carcinogenesis ,Herb ,Inula ,Mustard Plant ,Research Article - Abstract
Background General antiviral agents such as oseltamivir are associated with certain adverse effects and the emergence of resistance. This study investigated the phytochemical properties, antiviral activities, and safety of three herbs used in traditional Korean medicine. Methods Extracts of three medicinal herbs (Brassica juncea, Forsythia suspensa, and Inula britannica) were prepared using ethanol or water. The total phenolic, flavonoid, and saponin content, condensed tannin content, and reducing sugar content of the herb extracts were determined via phytochemical screening. Tandem mass analysis was performed using an ultra-performance liquid chromatography (UPLC)-electrospray ionization (ESI)-Q/Orbitrap instrument. Virus titrations were determined via tissue culture infective dose (TCID50) and cytotoxicity assays. Hemolysis and hepatotoxicity were measured to determine safety. Results Among the three medicinal herbs, F. suspensa showed the highest concentration of phenolic compounds, flavonoids, and saponins. The number of phytochemical compounds detected via tandem mass analysis of B. juncea, F. suspensa, and I. britannica was 5 (including sinigrin, m/z [M-H] = 358.02), 14 (including forsythoside A, m/z [M-H] = 623.19), and 18 (including chlorogenic acid, m/z [M-H] = 353.20), respectively. The antiviral effects of the B. juncea extracts (ethanol and water) and I. britannica extract (ethanol) were further investigated. The ethanol extract of B. juncea showed a 3 Log TCID50/25 μL virus titration reduction and the water extract showed a selectivity index of 13.668 against infected influenza H1N1 virus A/NWS/33. The B. juncea extracts did not show hemolysis activities and hepatotoxicity (I. britannica showed the most effective virus titration decrease, whereas its hemolytic and hepatotoxicity values were the most significantly different compared to the control. Despite the high concentration of phytochemicals detected in F. suspensa, the extract showed approximately 1 Log TCID50/25 μL at the highest concentration. Conclusion B. juncea may show antiviral effects against H1N1 in a host. In addition, B. juncea may also show decreased disadvantages compared to other antiviral agents.
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- 2019
10. Evaluation of the Quality of Yogurt Using Ginseng Extract Powder and Probiotic Lactobacillus plantarum NK181
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Hyung-Seok Yu, Jieun Jung, Na-Kyoung Lee, Hye Ji Jang, and Hyun-Dong Paik
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Streptococcus thermophilus ,Antioxidant ,DPPH ,medicine.medical_treatment ,antioxidant activity ,Titratable acid ,ginseng ,Article ,law.invention ,03 medical and health sciences ,Ginseng ,Probiotic ,chemistry.chemical_compound ,0404 agricultural biotechnology ,0302 clinical medicine ,law ,medicine ,Food science ,biology ,Chemistry ,physicochemical property ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,probiotic yogurt ,030221 ophthalmology & optometry ,Lactobacillus plantarum NK181 ,Animal Science and Zoology ,Fermentation ,Lactobacillus plantarum ,Food Science - Abstract
The objective of this study was to evaluate the composition, pH, titratable activity, microbial properties, and antioxidant effect of yogurt using ginseng extract powder (GEP), Lactobacillus plantarum NK181, and Streptococcus thermophilus as the starter culture. Different concentration of GEP (0%, 0.5%, 1%, 1.5%, and 2% (w/v)) were used in the yogurt. During yogurt fermentation, pH was decreased; however, titratable acidity and viable cell counts were increased. The addition of GEP to yogurt led to a decrease in moisture content and an increase in the fat, ash, and total solids content. The antioxidant effect using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene bleaching, and ferric reducing antioxidant power (FRAP) assay gradually increased with added GEP. Overall, yogurt fermented with 1% GEP was acceptable in terms of cell viability and antioxidant effect. These results might provide information regarding development of ginseng dairy products with enhanced antioxidant activities and probiotic properties.
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- 2018
11. Antimicrobial and anti-biofilm effects of probiotic Lactobacillus plantarum KU200656 isolated from kimchi
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Jieun Lee, Na-Kyoung Lee, and Hyun-Dong Paik
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0106 biological sciences ,medicine.disease_cause ,01 natural sciences ,Applied Microbiology and Biotechnology ,Article ,Microbiology ,law.invention ,Probiotic ,Minimum inhibitory concentration ,0404 agricultural biotechnology ,Listeria monocytogenes ,law ,010608 biotechnology ,medicine ,Escherichia coli ,biology ,Chemistry ,Biofilm ,Pathogenic bacteria ,04 agricultural and veterinary sciences ,Antimicrobial ,biology.organism_classification ,040401 food science ,Lactobacillus plantarum ,Food Science ,Biotechnology - Abstract
The probiotic properties and anti-pathogenic effects of Lactobacillus plantarum KU200656 (KU200656) isolated from Korean fermented kimchi against Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, and Salmonella Typhimurium were investigated. KU200656 showed high tolerance to artificial gastric acid (99.48%) and bile salts (102.40%) and this strain was safe according to antibiotic sensitivity test; it could not produce harmful enzymes, including β-glucuronidase. KU200656 exhibited high adhesion (4.45%) to intestinal cells, HT-29 cells, with high cell surface hydrophobicity (87.31% for xylene and 81.11% for toluene). Moreover, KU200656 co-aggregated with pathogenic bacteria and exhibited antibacterial activity and anti-adhesion properties against pathogens. The cell-free supernatant (CFS) of KU200656 inhibited biofilm formation by pathogenic bacteria. In addition, half of the minimum inhibitory concentration of the KU200656 CFS downregulated the expression of biofilm-related genes, as determined by quantitative real-time PCR. Therefore, KU200656 was demonstrated to possess anti-pathogenic effects and have potential for use as probiotics in the food industry.
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- 2020
12. Potential neuroprotective effects of heat-killed Lactococcus lactis KC24 using SH-SY5Y cells against oxidative stress induced by hydrogen peroxide
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Hyun-Dong Paik, Na-Kyoung Lee, and Sung-Min Lim
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0106 biological sciences ,SH-SY5Y ,medicine.disease_cause ,01 natural sciences ,Applied Microbiology and Biotechnology ,Article ,law.invention ,Microbiology ,Probiotic ,0404 agricultural biotechnology ,Lactobacillus rhamnosus ,law ,010608 biotechnology ,Lactobacillus ,medicine ,biology ,Chemistry ,Lactococcus lactis ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,Lactobacillus plantarum ,Oxidative stress ,Bacteria ,Food Science ,Biotechnology - Abstract
The present study was an investigation of the neuroprotective effects of probiotic bacteria in SH-SY5Y neuroblastoma cells experiencing oxidative stress. The bacterial strains were: commercial Lactobacillus rhamnosus GG; two isolated bacterial strains (Lactobacillus delbrueckii KU200170 and Lactobacillus plantarum KU200661); and probiotic Lactococcus lactis KC24. To evaluate the neuroprotective effects of the bacteria, a conditioned medium (CM) was prepared using HT-29 cells cultured with the heat-killed probiotic strains. Of the bacterial strains tested, the oxidatively stressed SH-SY5Y cells were most viable when cultured with L. lactis KC24-CM. L. lactis KC24-CM promoted the expression of brain-derived neurotropic factor (BDNF) in the HT-29 cells. It also significantly increased BDNF expression and reduced the apoptosis-related Bax/Bcl-2 ratio in the oxidatively stressed SH-SY5Y cells. Therefore, L. lactis KC24 is a potential psychobiotic for use in the functional food industry.
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- 2020
13. Inula britannica Inhibits Adipogenesis of 3T3-L1 Preadipocytes via Modulation of Mitotic Clonal Expansion Involving ERK 1/2 and Akt Signaling Pathways
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Won-Ju Kim, Hyung-Seok Yu, Won-Young Bae, Hyun-Dong Paik, and Na-Kyoung Lee
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0301 basic medicine ,MAPK/ERK pathway ,anti-obesity ,Inula britannica ,lcsh:TX341-641 ,mitotic clonal expansion ,Article ,adipogenesis ,ERK 1/2 signaling pathways ,03 medical and health sciences ,0302 clinical medicine ,Akt signaling pathways ,Transcription factor ,Protein kinase B ,lipogenesis ,Nutrition and Dietetics ,biology ,Chemistry ,3T3-L1 ,Cell cycle ,biology.organism_classification ,Cell biology ,030104 developmental biology ,Adipogenesis ,030220 oncology & carcinogenesis ,Signal transduction ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
The flower of Inula britannica contains various phenolic compounds with prophylactic properties. This study aimed to determine the anti-adipogenic effect of an I. britannica flower aqueous extract (IAE) and its underlying mechanisms in the 3T3-L1 preadipocytes and to identify the phenolic compounds in the extract. Treatment with IAE inhibited the adipogenesis of 3T3-L1 preadipocytes by showing a dose-dependently suppressed intracellular lipid accumulation and significantly mitigated expression levels of lipogenesis- and adipogenesis-associated biomarkers including transcription factors. IAE exerted an anti-adipogenic effect through the modulation of the early phases of adipogenesis including mitotic clonal expansion (MCE). Treatment with IAE inhibited MCE by arresting the cell cycle at the G0/G1 phase and suppressing the activation of MCE-related transcription factors. Furthermore, IAE inhibited adipogenesis by regulating the extracellular signal-regulated kinase 1/2 and Akt signaling pathways. Protocatechuic acid, chlorogenic acid, kaempferol-3-O-glucoside, and 6-methoxyluteolin, which are reported to exhibit anti-adipogenic properties, were detected in IAE. Therefore, modulation of early phases of adipogenesis, especially MCE, is a key mechanism underlying the anti-adipogenic activity of IAE. In summary, the anti-obesity effects of IAE can be attributed to its phenolic compounds, and hence, IAE can be used for the development of anti-obesity products.
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- 2020
14. Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods
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Hyun-Dong Paik, Sung-Min Lim, Min-Jeong Cheon, and Na-Kyoung Lee
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0301 basic medicine ,Antioxidant ,Lactobacillus fermentum ,medicine.medical_treatment ,Catalysis ,law.invention ,Inorganic Chemistry ,lcsh:Chemistry ,03 medical and health sciences ,Probiotic ,Functional food ,law ,Lactobacillus ,kimchi ,medicine ,Food science ,Physical and Theoretical Chemistry ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,gut-brain-axis ,Lactobacillus buchneri ,030109 nutrition & dietetics ,biology ,Chemistry ,Organic Chemistry ,food and beverages ,General Medicine ,biology.organism_classification ,Computer Science Applications ,neuroprotective effect ,030104 developmental biology ,probiotics ,lcsh:Biology (General) ,lcsh:QD1-999 ,Toxicity ,lactobacillus buchneri ,Bacteria - Abstract
The purpose of this study was to evaluate the probiotic characteristics and neuroprotective effects of bacteria isolated from Korean fermented foods. Three bacterial strains (Lactobacillus fermentum KU200060, Lactobacillus delbrueckii KU200171, and Lactobacillus buchneri KU200793) showed potential probiotic properties, such as high tolerance against artificial gastric juice and bile salts, sensitivity to antibiotics, nonproduction of carcinogenic enzymes, and high adhesion to intestinal cells. Heat-killed L. fermentum KU200060 and L. buchneri KU200793 showed higher antioxidant activity than heat-killed L. delbrueckii KU200171. The conditioned medium (CM) was used to evaluate the reaction between HT-29 cells and each heat-killed strain. All CMs protected SH-SY5Y cells from 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. The expression of brain-derived neurotropic factor (BDNF) mRNA in HT-29 cells treated with CM containing heat-killed L. buchneri KU200793 was the highest. The CM significantly reduced the Bax/Bcl-2 ratio and increased BDNF mRNA expression in SH-SY5Y cells treated with MPP+. These results indicate that L. buchneri KU200793 can be used as a prophylactic functional food, having probiotic potential and neuroprotective effects.
- Published
- 2020
15. Inula britannica fermented with probiotic Weissella cibaria D30 exhibited anti-inflammatory effect and increased viability in RAW 264.7 cells
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Na-Kyoung Lee, Hyeong-Yeop Kim, Young-Ho Hong, Kyung-Hoon Chang, Won-Young Bae, Hyung-Seok Yu, and Hyun-Dong Paik
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0106 biological sciences ,medicine.drug_class ,Flavonoid ,01 natural sciences ,Applied Microbiology and Biotechnology ,Anti-inflammatory ,Article ,law.invention ,Probiotic ,0404 agricultural biotechnology ,law ,010608 biotechnology ,medicine ,Food science ,Viability assay ,Weissella cibaria ,chemistry.chemical_classification ,biology ,Chemistry ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,Bioavailability ,Inula britannica ,Fermentation ,Food Science ,Biotechnology - Abstract
The objective of this study was to increase the bioavailability of Inula britannica (IB) through fermentation with probiotic Weissella cibaria D30, and to evaluate the chemical composition, viability, and anti-inflammatory effect of fermented I. britannica (FIB). IB was fermented with W. cibaria D30 at 37 °C for 24 h. FIB increased total phenolic content and decreased total flavonoid content of IB. 1-O-acetylbritannilactone and ergolide production, which are associated with the viability, increased from 1.38 to 4.13 μg/mg, and decreased from 5.24 to 0.94 μg/mg, in the control and FIB, respectively. In addition, the cell viability of RAW264.7 cells increased when pretreated with 400 μg/mL FIB. FIB inhibited the production of nitric oxide and proinflammatory cytokines by inhibiting NF-κB and MAPKs pathways. Therefore, FIB with W. cibaria D30 reduced the toxicity and increased the anti-inflammatory properties. These results indicate that FIB is a potential beneficial bioactive agent for functional foods.
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- 2019
16. The prophylactic effect of probiotic Bacillus polyfermenticus KU3 against cancer cells
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Na-Kyoung Lee, Gi Hoon Jung, Sung-Ho Son, Eun Bi Jeon, Hyun-Dong Paik, and Joo-Yeon Lee
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medicine.drug_class ,Cell ,Medicine (miscellaneous) ,Biology ,Proinflammatory cytokine ,Microbiology ,law.invention ,HeLa ,Probiotic ,law ,medicine ,TX341-641 ,Carcinogen ,chemistry.chemical_classification ,Nutrition and Dietetics ,Bile acid ,Nutrition. Foods and food supply ,Probiotics ,Anti-inflammatory effect ,Bacillus polyfermenticus ,biology.organism_classification ,Enzyme ,medicine.anatomical_structure ,chemistry ,Cancer cell ,Anticancer effect ,Food Science - Abstract
Bacillus polyfermenticus KU3 was isolated from kimchi , a Korean dish made from fermented vegetables and its potential probiotic characteristics were investigated. The spore cell of B. polyfermenticus KU3 was highly resistant to artificial gastric juice and survived for 24 h in artificial bile acid. B. polyfermenticus KU3 did not generate the carcinogenic enzymes, β-glucosidase, N-acetyl-β-glucosaminidase, and β-glucuronidase, and adhered strongly to HT-29 human intestinal epithelial cell lines. Using the [3–4,5-dimethylthiazol-2-yl]-2,5-diphenyletrazolium bromide assay, we found that B. polyfermenticus KU3 strongly inhibited the proliferation of cancer cells such as HeLa, LoVo, HT-29, AGS, and MCF-7 cells. The supernatant of B. polyfermenticus KU3 had an anticancer effect against HeLa and LoVo cells. Conversely, the proliferation of normal MRC-5 cells was not inhibited. We also demonstrated the anti-inflammatory activity of B. polyfermenticus KU3 under inflammatory conditions, as shown by the reduction in nitric oxide and proinflammatory cytokines (TNF-α, IL-10, TGF-β2, and COX-2). These results demonstrate the probiotic characteristics of B. polyfermenticus KU3 and provide evidence for the effect of this bacterium against various cancer cells.
- Published
- 2015
17. Predicting the Natural Course of Hemivertebra in Early Childhood: Clinical Significance of Anteroposterior Discordance Based on Three-dimensional Analysis.
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Sam Yeol Chang, Yunjin Nam, Jeongik Lee, Na-Kyoung Lee, Bong-Soon Chang, Choon-Ki Lee, Hyoungmin Kim, Chang, Sam Yeol, Nam, Yunjin, Lee, Jeongik, Lee, Na-Kyoung, Chang, Bong-Soon, Lee, Choon-Ki, and Kim, Hyoungmin
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- 2019
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18. Antibacterial Effect of a Mixed Natural Preservative against Listeria monocytogenes on Lettuce and Raw Pork Loin.
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HWAN HEE YU, MYUNG WOOK SONG, YE JI SONG, NA-KYOUNG LEE, and HYUN-DONG PAIK
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LISTERIA monocytogenes ,LETTUCE ,PORK ,COST of living ,COOKING - Abstract
A mixed natural preservative, including grapefruit seed extract (GSE), cinnamaldehyde (CA), and nisin, was investigated for the reduction of Listeria monocytogenes growth on lettuce and raw pork loin. The MIC of each natural preservative was investigated for L. monocytogenes strains tested. Following central composite design, lettuce and pork loin were inoculated with a cocktail of three strains of L. monocytogenes (ATCC 15313, H7962, and NADC 2045 [Scott A]) and treated with the mixed natural preservative that included GSE (0.64 to 7.36 ppm), CA (1.6 to 18.4 ppm), and nisin (0.48 to 5.5 ppm). The MIC of GSE was 31.25 ppm in tested L. monocytogenes strains, and of CA was 500 and 1,000 ppm in L. monocytogenes ATCC 15313 and the other L. monocytogenes strains, respectively. The MIC of nisin was 250 ppm. The R
2 value of this model was more than 0.9, and the lack of fit was not significant. The mixed natural preservative showed a synergistic antimicrobial effect and reduced the growth of L. monocytogenes by 4 to 5 log CFU/g on lettuce. In addition, the reduction of L. monocytogenes on pork loin was 3 log CFU/g. The mixed natural preservative, which consisted of GSE (6 to 8 ppm), CA (15 to 20 ppm), and nisin (5 to 6 ppm), increased the antibacterial effect against L. monocytogenes. These results suggest that the use of the mixed natural preservative could reduce the economic cost of food preparation, and response surface methodology is considered effective when measuring synergy among antimicrobials. [ABSTRACT FROM AUTHOR]- Published
- 2019
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19. Verification of the Optimal Chest Compression Depth for Children in the 2015 American Heart Association Guidelines: Computed Tomography Study.
- Author
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Yong Hwan Kim, Jun Ho Lee, Kwang Won Cho, Dong Woo Lee, Mun Ju Kang, Kyoung Yul Lee, Joung Hun Byun, Young Hwan Lee, Seong Youn Hwang, Na Kyoung Lee, Kim, Yong Hwan, Lee, Jun Ho, Cho, Kwang Won, Lee, Dong Woo, Kang, Mun Ju, Lee, Kyoung Yul, Byun, Joung Hun, Lee, Young Hwan, Hwang, Seong Youn, and Lee, Na Kyoung
- Published
- 2018
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20. Assessment of a novel deep learning-based software developed for automatic feature extraction and grading of radiographic knee osteoarthritis
- Author
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Ji Soo Yoon, Chang-Jin Yon, Daewoo Lee, Jae Joon Lee, Chang Ho Kang, Seung-Baik Kang, Na-Kyoung Lee, and Chong Bum Chang
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Deep learning ,Artificial intelligence ,Knee osteoarthritis ,Joint space narrowing ,Kellgren & Lawrence classification ,KL grade ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background The Kellgren-Lawrence (KL) grading system is the most widely used method to classify the severity of osteoarthritis (OA) of the knee. However, due to ambiguity of terminology, the KL system showed inferior inter- and intra-observer reliability. For a more reliable evaluation, we recently developed novel deep learning (DL) software known as MediAI-OA to extract each radiographic feature of knee OA and to grade OA severity based on the KL system. Methods This research used data from the Osteoarthritis Initiative for training and validation of MediAI-OA. 44,193 radiographs and 810 radiographs were set as the training data and used as validation data, respectively. This AI model was developed to automatically quantify the degree of joint space narrowing (JSN) of medial and lateral tibiofemoral joint, to automatically detect osteophytes in four regions (medial distal femur, lateral distal femur, medial proximal tibia and lateral proximal tibia) of the knee joint, to classify the KL grade, and present the results of these three OA features together. The model was tested by using 400 test datasets, and the results were compared to the ground truth. The accuracy of the JSN quantification and osteophyte detection was evaluated. The KL grade classification performance was evaluated by precision, recall, F1 score, accuracy, and Cohen's kappa coefficient. In addition, we defined KL grade 2 or higher as clinically significant OA, and accuracy of OA diagnosis were obtained. Results The mean squared error of JSN rate quantification was 0.067 and average osteophyte detection accuracy of the MediAI-OA was 0.84. The accuracy of KL grading was 0.83, and the kappa coefficient between the AI model and ground truth was 0.768, which demonstrated substantial consistency. The OA diagnosis accuracy of this software was 0.92. Conclusions The novel DL software known as MediAI-OA demonstrated satisfactory performance comparable to that of experienced orthopedic surgeons and radiologists for analyzing features of knee OA, KL grading and OA diagnosis. Therefore, reliable KL grading can be performed and the burden of the radiologist can be reduced by using MediAI-OA.
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- 2023
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21. Bioactive compounds of probiotic Saccharomyces cerevisiae strains isolated from cucumber jangajji
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Na-Kyoung Lee, Ji-Young Hong, Sung-Hun Yi, Sang-Pil Hong, Jang-Eun Lee, and Hyun-Dong Paik
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Saccharomyces cerevisiae ,Bioactive compound ,β-Glucan ,Polyamine ,Probiotic property ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The bioactive compounds and probiotic properties of three Saccharomyces cerevisiae strains isolated from cucumber jangajji were investigated. The β-glucan contents of S. cerevisiae KU200270, KU200280, and KU200284 were 4.09%, 7.45%, and 13.97%, respectively, and the glutathione contents of the strains were in the range of 3.19–6.03 mg/g dry cells. The l-ornithine and spermidine contents were 2.98–4.29 and 4.29–4.55 mg/g dry cells, respectively. All strains showed the ability to protect DNA from oxidative damage. The S. cerevisiae strains were highly resistant to both artificial gastric and bile juices. The epithelial cell adhesion of S. cerevisiae KU200284 was the highest at 16.38%, while that of the other strains was >5%. The S. cerevisiae strains produced esterase, leucine arylamidase, acid phosphatase, and α-glucosidase and showed no toxicity toward MRC-5 cells. These results suggest that the isolated S. cerevisiae strains have potential as probiotics and can be used starter cultures for functional foods.
- Published
- 2019
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22. Antimicrobial and Antibiofilm Effect of ε-Polylysine against Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli in Tryptic Soy Broth and Chicken Juice
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Do-Un Lee, Yeong Jin Park, Hwan Hee Yu, Suk-Chae Jung, Jung-Hee Park, Dae-Hee Lee, Na-Kyoung Lee, and Hyun-Dong Paik
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ε-polylysine ,Salmonella Enteritidis ,Listeria monocytogenes ,Escherichia coli ,antimicrobial effect ,antibiofilm ,Chemical technology ,TP1-1185 - Abstract
ε-Polylysine (ε-PL) is a safe food additive that is used in the food industry globally. This study evaluated the antimicrobial and antibiofilm activity of antibacterial peptides (ε-PL) against food poisoning pathogens detected in chicken (Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli). The results showed that minimum inhibitory concentrations (MICs) ranged between 0.031–1.0 mg/mL, although most bacterial groups (75%) showed MICs of 1.0 mg/mL. The reduction in the cell viability of pathogens due to ε-PL depended on the time and concentration, and 1/2 × MIC of ε-PL killed 99.99% of pathogens after 10 h of incubation. To confirm biofilm inhibition and degradation effects, crystal violet assay and confocal laser scanning microscopy (CLSM) were used. The biofilm formation rates of four bacterial groups (Salmonella, Listeria, E. coli, and multi-species bacteria) were 10.36%, 9.10%, 17.44%, and 21.37% at 1/2 × MIC of ε-PL, respectively. Additionally, when observed under a CLSM, ε-PL was found to induce biofilm destruction and bacterial cytotoxicity. These results demonstrated that ε-PL has the potential to be used as an antibiotic and antibiofilm material for chicken meat processing.
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- 2021
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23. CHARACTERISTICS OF WHEY PROTEIN HYDROLYSATES FROM CHEESE WHEY, FAVORS ON VARIOUS FOOD APPLICATION.
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RENDA KANKANAMGE, CHATURIKA JEEWANTHI, HYUN-DONG PAIK, MYEONG-HEE KIM, NA-KYOUNG LEE, SOO-YEON KIM, and YOH CHANG YOON
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WHEY proteins , *HYDROLASES , *SOLUBILITY , *CHEESE , *PROTEOLYTIC enzymes , *PEPSIN - Abstract
This study was conducted to investigate the production of whey protein hydrolysates, examining the physiochemical properties with five enzyme types named alcalase, protease S, protease M, trypsin, and pepsin. Whey protein concentrate was adjusted by ultrafiltration, increasing the whey content to 135% that of initial levels. The hydrolysates have been shown to improve the characteristics of a number of food products, and the type of enzyme has a considerable influence on the end result of hydrolysates production. Bulk density, solubility, NPN, foaming capacity, and the degree of hydrolysis were increased with hydrolysis time. Maximum bulk density was shown by protease S. Pepsin and alcalase gradually increased the foaming capacity, resulting in a comparatively lower pH and a lower degree of hydrolysis. The highest degree of hydrolysis was shown by protease M. The highest NPN value was provided by pepsin, which was much greater than that of other enzymes. There was no significant difference in NPN according to the enzyme type applied. All hydrolysates in alkaline media were shown more than 50% solubility. HMF contents were also shown an obvious difference with the enzyme type. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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