18 results on '"Multistrain probiotics"'
Search Results
2. Probiotics modulation of the endotoxemic effect on the gut and liver of the lipopolysaccharide challenged mice.
- Author
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Babu, Gyan and Mohanty, Banalata
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GUT microbiome , *BACTERIAL population , *LABORATORY mice , *PEPTIDES , *OXIDATIVE stress , *PROBIOTICS - Abstract
AbstractThe multistrain probiotics’ efficacy in ameliorating the endotoxemic effect in Lipopolysaccharide (LPS) challenged mice was evaluated with the agonist of anti-inflammatory peptide, neurotensin (NTS), especially targeting the inflammation of the gut and liver. Swiss Albino Mice (Female, 8 weeks old) were maintained in eight groups: Group I as Control, Group II-Group V were exposed to intraperitoneal (i.p.) LPS (1 mg/kg bw) for 5 days. After that, Group III and Group VI were administered probiotics orally (0.6 gm/kg bw/day), Group IV and Group VII with NTS receptor 1 (NTSR1) agonist PD149163 (50 µg/kg bw/day i.p.), and Group V and Group VIII co-administered with probiotics and PD149163 for 28 days. Group II (LPS-exposed) was maintained without any further treatment; mice of all the groups were sacrificed at day 34. In the LPS-exposed mice, endotoxemia was distinct from a significant (
P < 0.001) increase of plasma pro-inflammatory cytokines (TNF-α; IL-6), a decrease of anti-inflammatory cytokine (IL-10), oxidative stress, and inflammation of the gut and liver. Increased serum transaminases indicated hepatic inflammation. A decreased population of thebifidobacteria and increasedclostridia indicated microbiota dysbiosis. Probiotics when used as an adjunct along with PD149163 have shown better efficacy in inflammation modulation as reflected in the significantly decreased (P < 0.001) inflammatory mediators, oxidative stress, restoration of the beneficial bacterial population, along with a significant reduction in histopathological scores of the gut and the liver than when used alone. This study suggests probiotics could be used as an adjunct in clinical practice along with anti-inflammatory drugs for better therapeutic efficacy. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
3. Multistrain Probiotics Alleviate Diarrhea by Modulating Microbiome-Derived Metabolites and Serotonin Pathway
- Author
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Jeong, Jin-Ju, Jin, Yoo-Jeong, Ganesan, Raja, Park, Hee Jin, Min, Byeong Hyun, Jeong, Min Kyo, Yoon, Sang Jun, Choi, Mi Ran, Sharma, Satya Priya, Jang, You Jin, Min, Uigi, Lim, Jong-Hyun, Na, Kyeong Min, Choi, Jieun, Han, Sang Hak, Ham, Young Lim, Lee, Do Yup, Kim, Byung-Yong, and Suk, Ki Tae
- Published
- 2024
- Full Text
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4. Benefits of multistrain bacteria formulations for health
- Author
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L. Laterza, G. Gibiino, F. Scaldaferri, and A. Gasbarrini
- Subjects
Multistrain probiotics ,Gastrointestinal disease ,Gut microbiota ,VSL#3 ,Irritable bowel syndrome ,H. pylori ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Gut microbiota dysfunction is associated with multiple gastrointestinal and extra-gastrointestinal diseases, thus the possibility of its modulation with prebiotics, probiotics and symbiotics has gained interest in the last years. Many probiotics are available on the market and products characterized by combination of multiple strains have been proposed as particularly effective. However, evidences supporting their efficacy are often inadequate and not homogenous. We reviewed available data on clinical efficacy of multistrain probiotics and symbiotics in gastrointestinal diseases in adults, focusing on data from randomised, double-blind, placebo-controlled clinical trials. Twenty-nine multistrain products satisfied inclusion criteria. The principal areas of application are irritable bowel syndrome, Helicobacter pylori eradication and antibiotic-associated diarrhoea. The most represented probiotic species in the different probiotic formulations was L. acidophilus. The combination of L. paracasei, L. plantarum, L. acidophilus, L. delbrueckii subsp. bulgaricus, B. longum, B. infantis, B. breve and S. thermophilus (VSL#3) showed the highest number of RCTs.
- Published
- 2018
- Full Text
- View/download PDF
5. Variability in Probiotic Formulations Revealed by Proteomics and Physico-chemistry Approach in Relation to the Gut Permeability.
- Author
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Razafindralambo, H., Correani, V., Fiorucci, S., and Mattei, B.
- Abstract
Variability in the efficacy, safety, and quality of probiotic formulations depends on many factors, including process conditions used by manufacturers. Developing reliable analytical tools is therefore essential to quickly monitor manufacturing differences in probiotic samples for their quality assessment. Here, multi-strain probiotics from two production sites and countries were investigated by proteomics and physico-chemistry approaches in relation to the protective effect on gut barrier. Proteomic analyses showed differences in protein abundances, identities, and origins of two series of VSL#3 samples from different sites. Even though both formulations were qualitatively similar in thermal and colloidal profiles, significant differences were quantitatively observed in terms of maximum decomposition temperature Tmax (p < 0.05) and phase transition temperature Tm (p < 0.01). Such variability in physical and biochemical features impacts on probiotic functionalities and translates into a differential modulation of gut permeability in mice. Physico-chemical scans provide coherent data with proteomics and represent a new tool for time and cost effective quality control of probiotic-based products. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
6. The effect of supplementation of multistrain probiotic preparation in combination with vitamins and minerals to the basal diet on the growth performance, carcass traits, and physiological response of broilers
- Author
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Sugiharto Sugiharto, Isroli Isroli, Turrini Yudiarti, and Endang Widiastuti
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broiler chicks ,growth rate ,minerals ,multistrain probiotics ,physiological status ,vitamins ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Aim: The aim of the present study was to investigate the effect of supplementation of multistrain probiotic preparation in combination with vitamins and minerals to the broiler chicken diets on their growth performance, hematological parameters, and carcass traits. Materials and Methods: Two hundred and eighty-eight Lohmann 1-day-old broiler chicks were randomly allocated to four groups, i.e., control (without additional supplementation) and three experimental treatments where basal diet was enriched by 0.1%, 0.5%, or 1% of multistrain probiotic preparation in combination with vitamins and minerals, respectively. Blood sampling was conducted on day 28, while the selected organs and eviscerated carcasses were collected on day 42. Results: Dietary supplementation did not affect (p>0.05) the final body weight, feed intake, and feed conversion ratio of broilers. Supplementation by 0.1% and 0.5% of multistrain probiotic preparation in combination with vitamins and minerals reduced (p≤0.05) heart relative weight of broilers. Dietary supplementation tended (p=0.07) to increase the relative weight of ileum and pancreas of broilers. Supplemented birds had lower (p≤0.05) numbers of leukocytes and eosinophils compared to unsupplemented birds. There were tendencies that supplementation of multistrain probiotics in combination with vitamins and minerals resulted in lower (p=0.07) counts of lymphocytes and heterophils when compared with no supplementation. Supplementation by 0.5% of multistrain probiotics in combination with vitamins and minerals resulted in lower (p≤0.05) serum concentration of uric acid when compared with control. There was no significant effect of dietary supplementation on carcass traits, pH, and drip loss of broiler breast muscles. Conclusion: Dietary supplementation of commercial broiler feeds with 0.5% of multistrain probiotic preparation in combination with vitamins and minerals was potential to improve digestive functions and physiological status of broiler chickens.
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- 2018
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7. New insights on strain-specific impacts of probiotics on insulin resistance: evidence from animal study.
- Author
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Kobyliak, Nazarii, Falalyeyeva, Tetyana, Tsyryuk, Olena, Eslami, Majid, Kyriienko, Dmytro, Beregova, Tetyana, and Ostapchenko, Liudmila
- Subjects
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INSULIN resistance , *MONOSODIUM glutamate , *BIFIDOBACTERIUM , *LACTOBACILLUS casei , *ACETOBACTER , *PROBIOTICS - Abstract
Background and aims: сomparative animal study of effectiveness of intermittent administration of lyophilized single-, three- and alive multistrain probiotic in short courses on insulin resistance (IR) in rats with experimental obesity. Methods: 70 rats were divided into 7 groups (n = 10 in each). Rats of group I were left intact. Newborn rats in groups II-VII were administered monosodium glutamate (MSG) (4 mg/g) by injection. Rats in group II (MSG-obesity group) were left untreated. The rats in groups III-V received lyophilized mono-probiotics B.animalis VKL, B.animalis VKB, L.casei IMVB-7280 respectively. The rats in group VI received all three of these probiotic strains mixed together. Group VII was treated with multi-probiotic "Symbiter", containing 14 different live probiotic strains (Lactobacillus, Bifidobacterium, Propionibacterium, Acetobacter genera). Results: Treatment of newborn rats with MSG lead to the development of obesity in all MSG-obesity rats and up to 20–70% after probiotic administration. Additions to probiotic composition, with preference to alive strains (group VII), led to significantly lower rates of obesity, decrease in HOMA-IR (p < 0.001), proinflammatory cytokines levels – IL-1β (p = 0.003), IL-12Bp40 (p < 0.001) and elevation of adiponectin (p = 0.003), TGF-β (p = 0.010) in comparison with MSG-obesity group. Analysis of results in groups treated with single-strain probiotics (groups III-V) shows significant decrease in HOMA-IR, but changes were less pronounced as compared to mixture groups and did not achieve intact rats level. Other metabolic parameters were not affected significantly by single strains. Conclusion: Our findings provide major clues for how to design and use probiotics with more efficient compositions in obesity and IR management and may bring new insights into how host-microbe interactions contribute to such protective effects. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
8. Benefits of multistrain bacteria formulations for health.
- Author
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Laterza, L., Gibiino, G., Scaldaferri, F., and Gasbarrini, A.
- Abstract
Gut microbiota dysfunction is associated with multiple gastrointestinal and extra-gastrointestinal diseases, thus the possibility of its modulation with prebiotics, probiotics and symbiotics has gained interest in the last years. Many probiotics are available on the market and products characterized by combination of multiple strains have been proposed as particularly effective. However, evidences supporting their efficacy are often inadequate and not homogenous. We reviewed available data on clinical efficacy of multistrain probiotics and symbiotics in gastrointestinal diseases in adults, focusing on data from randomised, double-blind, placebo-controlled clinical trials. Twenty-nine multistrain products satisfied inclusion criteria. The principal areas of application are irritable bowel syndrome, Helicobacter pylori eradication and antibiotic-associated diarrhoea. The most represented probiotic species in the different probiotic formulations was L. acidophilus . The combination of L. paracasei, L. plantarum, L. acidophilus, L. delbrueckii subsp. bulgaricus, B. longum, B. infantis , B. breve and S. thermophilus (VSL#3) showed the highest number of RCTs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
9. Effects of multistrain probiotics on growth performance, nutrient digestibility, blood profiles, faecal microbial shedding, faecal score and noxious gas emission in weaning pigs.
- Author
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Lan, R. X., Lee, S. I., and Kim, I. H.
- Subjects
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PROBIOTICS , *GASES from plants , *FECES , *NUTRITION counseling , *THIOLS ,PHYSIOLOGICAL effect - Abstract
This experiment was conducted to investigate the efficacy of multistrain probiotics in weaning pigs. A total of 125 28-day-old weaning pigs [(Landrace × Yorkshire) × Duroc] with an initial average body weight ( BW) of 7.26 ± 0.76 kg were randomly allotted into 5 treatments, 5 replicate pens/treatment with 5 pigs/pen for 42-day experiment. Dietary treatments were as follows: CON, basal diet; PC1, CON + 0.01% multistrain probiotics; PC2, CON + 0.03% multistrain probiotics; PC3, CON + 0.06% multistrain probiotics; PC4, CON + 0.1% multistrain probiotics. On day 14, pigs fed the PC4 diet had higher BW gain than pigs fed the CON diet. On day 42, pigs fed multistrain probiotics supplementation diets had higher BW gain than pigs fed the CON diet. From days 1 to 14, pigs fed the PC2, PC3 and PC4 diets had higher (p < 0.05) ADG than pigs fed the CON diet. From day 15 to 42, pigs fed the multistrain probiotics supplementation diets had higher (p < 0.05) average daily gain ( ADG) and gain: feed ratio (G:F) than pigs fed the CON diet. In the overall period, pigs fed the multistrain probiotics supplementation diets had higher (p < 0.05) ADG and pigs fed the PC2 and PC4 diets had higher (p < 0.05) G:F than pigs fed the CON diet. On day 42, pigs fed the PC4 diet had higher (p < 0.05) apparent total tract digestibility ( ATTD) of dry matter ( DM), nitrogen (N) and gross energy ( GE), faecal Lactobacillus counts and lower (p < 0.05) E. coli counts and NH3 emission than pigs fed the CON diet. Pigs fed the multistrain probiotics supplementation diets had lower (p < 0.05) H2S and total mercaptans emissions than pigs fed the CON diet. Conclusions, dietary supplementation with 0.1% probiotics improved growth performance, nutrition digestibility and intestinal microflora balance and decreased faecal noxious gas emissions in weaning pigs. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
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10. Multistrain probiotic production by co-culture fermentation in a lab-scale bioreactor.
- Author
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Jangra, Manoj, Belur, Prasanna D., Oriabinska, Larysa B., and Dugan, Olexii M.
- Subjects
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PROBIOTICS , *FERMENTATION , *BIOREACTORS , *LACTOBACILLUS plantarum , *LACTOBACILLUS delbrueckii - Abstract
Most commercial probiotic products intended for pharmaceutical applications consist of combinations of probiotic strains and are available in various forms. The development of co-culture fermentation conditions to produce probiotics with the correct proportion of viable microorganisms would reduce multiple operations and the associated costs. The aim of this study was to develop a fermentation medium and process to achieve biomass comprising the desired proportion of two probiotic strains in co-culture. Initially, a quantification medium was developed, and the method was optimized to allow the quantification of each strain's biomass in a mixture. The specific growth rates of Lactobacillus delbrueckii spp. bulgaricus and Lactobacillus plantarum were determined in media with different carbon sources. The inoculum volume was optimized to achieve equal proportion of biomass in co-culture fermentation in test tubes. Next, fermentation was carried out in a 3-L bioreactor. A biomass concentration of 2.06 g/L, with L. delbrueckii spp. bulgaricus and L. plantarum in the ratio of 47%:53% (by weight), was achieved with concomitant production of 12.69 g/L of lactic acid in 14 h. The results show that with careful manipulation of process conditions, it is possible to achieve the desired proportion of individual strains in the final biomass produced by co-culture fermentation. This process may serve as a model to produce multistrain probiotic drugs at industrial scale. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
11. Comparative experimental investigation on the efficacy of mono- and multiprobiotic strains in non-alcoholic fatty liver disease prevention.
- Author
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Kobyliak, Nazarii, Falalyeyeva, Tetyana, Virchenko, Oleksandr, Mykhalchyshyn, Galyna, Bodnar, Petro, Spivak, Mykola, Yankovsky, Dmytro, Beregova, Tetyana, and Ostapchenko, Lyudmyla
- Subjects
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FATTY liver prevention , *RATS , *PROBIOTICS , *MONOSODIUM glutamate , *LACTOBACILLUS casei , *THERAPEUTIC use of probiotics , *ANIMAL experimentation , *BIFIDOBACTERIUM , *BIOLOGICAL models , *COMPARATIVE studies , *FATTY liver , *FLAVORING essences , *FREEZE-drying , *GENETIC disorders , *GRAM-positive bacteria , *LACTOBACILLUS , *LIPID metabolism disorders , *LIVER , *RESEARCH methodology , *MEDICAL cooperation , *OBESITY , *RESEARCH , *TRIGLYCERIDES , *EVALUATION research , *GRAM-negative aerobic bacteria - Abstract
Background: To investigate the efficacy of different probiotic strains, their combinations and forms (alive or lyophilized) in nonalcoholic fatty liver disease (NAFLD) prevention.Methods: In this study, 70 rats have been used divided into 7 groups of 10 animals in each: I - intact rats, II-VII - rats with monosodium glutamate (MSG)-induced NAFLD. Rats with NAFLD were untreated (group II, MSG-obesity group) and treated with probiotics (groups III-VII). In order to develop NAFLD, newborn rats of groups II-VII were injected with a solution of monosodium glutamate (MSG) (4 mg/g) subcutaneously (s.c.) at 2nd,4th, 6th, 8th,10th postnatal day. The groups III-V received lyophilized monoprobiotics B. animalis VKL, B. animalis VKB, L.casei IMVB-7280, respectively. The group VI received 2.5 ml/kg of an aqueous solution of a mixture of the three probiotic strains (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, Bifidobacterium animalis VKB) at a dose of 50 mg/kg (5 × 10(9) CFU/kg) (g) (intragastrically). The group VII was treated with multiprobiotic "Symbiter" containing biomass of 14 alive probiotic strains (Lactobacillus + Lactococcus (6 × 10(10) CFU/g), Bifidobacterium (1 × 10(10)/g), Propionibacterium (3 × 10(10)/g), Acetobacter (1 × 10(6)/g)) at a dose of 140 mg/kg (1.4 × 10(10) CFU/kg). The treatment with probiotics was started at the age of 1 month. There were 3 courses of treatment, each included 2-week administration and 2-week break. All parameters were measured in 4-month aged rats.Results: Introduction of MSG during the neonatal period leads to the NAFLD development in the 4-months old rats. For steatosis degree there was no significant difference between MSG-obesity group and lyophilized monocomponent probiotics groups (III-V). The highest manifestation of steatosis was observed for B. animalis VKL group (2.0 ± 0.25) as compared to B. animalis VKB (1.70 ± 0.21) and L. casei IMVB-7280 (1.80 ± 0.20). The steatosis score changes between all monoprobiotics groups (III-V) were insignificant. Administration from birth of both alive (VII) and lyophilized (VI) probiotic mixture lead to a significant decrease by 69.5 % (p < 0.001) and 43.5 % (p < 0.025) of steatosis score respectively as compared to the MSG-obesity group (2.3 ± 0.21 %). For both alive and lyophilized probiotic mixtures, reduction of lobular inflammation was observed. These histological data were confirmed by the significant decrease of total lipids and triglycerides content in the liver approximately by 22-25 % in groups treated with probiotic mixtures (VI, VII) compared to the MSG-obesity group.Conclusion: We established failure of NAFLD prevention with lyophilized monoprobiotic strains and the efficacy of probiotic mixture with the preference of alive probiotic strains. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
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12. Do probiotic preparations for humans really have efficacy?
- Author
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Mikelsaar, Marika, Lazar, Veronica, Onderdonk, Andrew Bruce, and Donelli, Gianfranco
- Subjects
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PROBIOTICS , *MICROBIAL ecology , *PHYSIOLOGICAL effects of antibiotics , *DIARRHEA , *THERAPEUTICS , *INFLAMMATORY bowel disease treatment , *IMMUNE system - Abstract
Microbial ecology studies have provided convincing evidence for the crosstalk between members of the autochthonous microbiota and the host immune system. The tight interrelationship between microbiota and host mucosal cells are mediated by microbial metabolites that are responsible for bacterial cell-to-cell communication by quorum-sensing mechanisms and also through the activation of eukaryotic cells following secretion of host defensins and modulation of cytokine expression profiles. All these host functions can be positively influenced by probiotic bacteria of human origin. However, few requirements for evaluating these strains for use in humans have been set according to their composition and metabolic activity. In this article, we have reported the scientific data published to date on the advantages of either mono- or multispecies probiotic products based on the outcome of the most significant clinical trials. According to published clinical trials, the efficacy of probiotic intervention for infectious or antimicrobial treatment-induced diarrhea, caused by different opportunistic bacterial or viral pathogens, was 48%. The probiotic preparations' efficacy for silencing the clinical symptoms of IBS was 75% and for attenuating the inflammatory response during IBD was 83% whereas multistrain probiotics appear to have better efficacy. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
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13. The effects of fermented milks with simple and complex probiotic mixtures on the intestinal microbiota and immune response of healthy adults and children
- Author
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Zanini, Katia, Marzotto, Marta, Castellazzi, Annamaria, Borsari, Andrea, Dellaglio, Franco, and Torriani, Sandra
- Subjects
- *
LACTOBACILLUS casei , *KILLER cells , *DAIRY products , *YOGURT - Abstract
Abstract: This study compared the effect of two functional fermented milks with simple and complex probiotic mixtures on the microbiota and some immune system functions of healthy adults and children. The complex yogurt, containing Lactobacillus casei and other strains of lactobacilli and bifidobacteria, and the simple yogurt, containing only L. casei, were administered daily to healthy volunteers (41 adults and 36 children) for a period of 4 weeks. Microbiological and molecular analyses of faecal samples indicated that the complex product induced a larger increase in the lactic acid bacteria number, compared with the simple product, without significant alterations of the autochthonous species composition. Of the probiotic strains, L. casei showed the best survival rate in faeces. The influences of both products on the immune system were similar, with increase of NK activity and proliferative response to Candida albicans in adults and secretory-IgA in children saliva, indicating that immunomodulating properties were probably supported by common traits in the two products. [Copyright &y& Elsevier]
- Published
- 2007
- Full Text
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14. Variability in Probiotic Formulations Revealed by Proteomics and Physico-chemistry Approach in Relation to the Gut Permeability
- Author
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Virginia Correani, Stefano Fiorucci, Hary Razafindralambo, and Benedetta Mattei
- Subjects
0301 basic medicine ,Proteome ,030106 microbiology ,Intestinal permeability ,Proteomics ,Inbred C57BL ,Microbiology ,Multistrain probiotics ,Permeability ,law.invention ,03 medical and health sciences ,Probiotic ,Mice ,law ,Gut permeability ,medicine ,Animals ,Food science ,Intestinal Mucosa ,Thermal decomposition ,Molecular Biology ,Colloidal properties ,Transition phase ,Differential modulation ,Gut barrier ,Quality assessment ,Chemistry ,Probiotics ,medicine.disease ,Process conditions ,Mice, Inbred C57BL ,030104 developmental biology ,Molecular Medicine - Abstract
Variability in the efficacy, safety, and quality of probiotic formulations depends on many factors, including process conditions used by manufacturers. Developing reliable analytical tools is therefore essential to quickly monitor manufacturing differences in probiotic samples for their quality assessment. Here, multi-strain probiotics from two production sites and countries were investigated by proteomics and physico-chemistry approaches in relation to the protective effect on gut barrier. Proteomic analyses showed differences in protein abundances, identities, and origins of two series of VSL#3 samples from different sites. Even though both formulations were qualitatively similar in thermal and colloidal profiles, significant differences were quantitatively observed in terms of maximum decomposition temperature Tmax (p
- Published
- 2020
15. Effects of the combination of multistrain probiotics and Castanea crenata shell extract on growth performance, nutrient digestibility, fecal microbial shedding, meat quality, noxious gas emissions, and blood parameters in finishing pigs.
- Author
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Liu, Xiao, Kim, Seung Hee, and Kim, In Ho
- Subjects
- *
MEAT quality , *NUTRIENT density , *CASTANEA , *SWINE , *PROBIOTICS , *FEED additives - Abstract
• The objective of the present study was to determine the effects of dietary supplementation MPC on the performance in finishing pigs. • Dietary supplementation with MPC presented beneficial effects on growth performance, nutrient digestibility, Lactobacillus counts, meat quality, and noxious gas emissions in finishing pigs. • The MPC has potential positive effects as a feed additive in pigs industry. The objective of the present study was to determine the effects of dietary supplementation of the combination of multistrain probiotics and Castanea crenata shell extract (MPC) on growth performance, nutrient digestibility, fecal microbial shedding, meat quality, noxious gas emissions, and blood parameters in finishing pigs. A total of 150 pigs were used in a 10-weeks feeding trial. Dietary treatment groups were as follows: CON, basal diet; TRT1, basal diet + 0.1% MPC; and TRT2, basal diet + 0.2% MPC. On days 35 and 70, there was a significant linear increase (P <0.05) in body weight, and the ATTD of dry matter, nitrogen, and Lactobacillus counts, and a significant linear decrease (P <0.05) in excreta ammonia emission with increasing MPC in the diets. During days 0 to 35, average daily gain and gain to feed ratio showed a linear increase (P <0.05) with increasing MPC, while serum creatinine concentration showed a linear decrease (P <0.05) on days 35. On days 70, drip loss decreased linearly with MPC in diets on day 7, while loin muscle area was linearly increased (P <0.05). During the overall period, there was a significant linear increase (P <0.05) in average daily gain and gain to feed ratio with increasing MPC in diets. In conclusion, dietary MPC supplementation has beneficial effects on growth performance, nutrient digestibility, fecal microbial shedding, meat quality, noxious gas emissions, and blood parameters in finishing pigs. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
16. Comparative experimental investigation on the efficacy of mono- and multiprobiotic strains in non-alcoholic fatty liver disease prevention
- Author
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Nazarii Kobyliak, Dmytro Yankovsky, Ostapchenko Li, Galyna Mykhalchyshyn, Tetyana Beregova, Tetyana Falalyeyeva, Petro Bodnar, Mykola Ya Spivak, and Oleksandr Virchenko
- Subjects
0301 basic medicine ,Male ,Monosodium glutamate ,Gastroenterology ,law.invention ,chemistry.chemical_compound ,Probiotic ,law ,Lyophilized and alive probiotic strains ,Non-alcoholic Fatty Liver Disease ,Nonalcoholic fatty liver disease ,Lactococcus ,Sodium Glutamate ,Medicine ,Bifidobacterium ,biology ,Fatty liver ,General Medicine ,Monoprobiotic ,Bifidobacterium animalis ,Lacticaseibacillus casei ,Liver ,Research Article ,medicine.medical_specialty ,Lactobacillus casei ,Multistrain probiotics ,03 medical and health sciences ,Internal medicine ,NAFLD ,Acetobacter ,Animals ,Obesity ,Rats, Wistar ,Triglycerides ,business.industry ,Prevention ,Probiotics ,Propionibacterium ,medicine.disease ,biology.organism_classification ,Lipid Metabolism ,Rats ,Flavoring Agents ,Disease Models, Animal ,030104 developmental biology ,Freeze Drying ,chemistry ,Steatosis ,business - Abstract
Background To investigate the efficacy of different probiotic strains, their combinations and forms (alive or lyophilized) in nonalcoholic fatty liver disease (NAFLD) prevention. Methods In this study, 70 rats have been used divided into 7 groups of 10 animals in each: I – intact rats, II-VII – rats with monosodium glutamate (MSG)-induced NAFLD. Rats with NAFLD were untreated (group II, MSG-obesity group) and treated with probiotics (groups III–VII). In order to develop NAFLD, newborn rats of groups II–VII were injected with a solution of monosodium glutamate (MSG) (4 mg/g) subcutaneously (s.c.) at 2nd,4th, 6th, 8th,10th postnatal day. The groups III–V received lyophilized monoprobiotics B. animalis VKL, B. animalis VKB, L.casei IMVB-7280, respectively. The group VI received 2.5 ml/kg of an aqueous solution of a mixture of the three probiotic strains (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, Bifidobacterium animalis VKB) at a dose of 50 mg/kg (5 × 109 CFU/kg) (g) (intragastrically). The group VII was treated with multiprobiotic “Symbiter” containing biomass of 14 alive probiotic strains (Lactobacillus + Lactococcus (6 × 1010 CFU/g), Bifidobacterium (1 × 1010/g), Propionibacterium (3 × 1010/g), Acetobacter (1 × 106/g)) at a dose of 140 mg/kg (1.4 × 1010 CFU/kg). The treatment with probiotics was started at the age of 1 month. There were 3 courses of treatment, each included 2-week administration and 2-week break. All parameters were measured in 4-month aged rats. Results Introduction of MSG during the neonatal period leads to the NAFLD development in the 4-months old rats. For steatosis degree there was no significant difference between MSG-obesity group and lyophilized monocomponent probiotics groups (III–V). The highest manifestation of steatosis was observed for B. animalis VKL group (2.0 ± 0.25) as compared to B. animalis VKB (1.70 ± 0.21) and L. casei IMVB-7280 (1.80 ± 0.20). The steatosis score changes between all monoprobiotics groups (III–V) were insignificant. Administration from birth of both alive (VII) and lyophilized (VI) probiotic mixture lead to a significant decrease by 69.5 % (p
- Published
- 2016
17. The effects of fermented milks with simple and complex probiotic mixtures on the intestinal microbiota and immune response of healthy adults and children
- Author
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Franco Dellaglio, Sandra Torriani, Katia Zanini, Marta Marzotto, Andrea Borsari, and A. M. Castellazzi
- Subjects
Lactobacillus casei ,Saliva ,NK cells ,Applied Microbiology and Biotechnology ,Multistrain probiotics ,Microbiology ,law.invention ,chemistry.chemical_compound ,Probiotic ,fluids and secretions ,Immune system ,law ,Candida albicans ,PCR-DGGE ,biology ,Healthy humans ,food and beverages ,Lactobacillaceae ,biology.organism_classification ,Lactic acid ,Secretory IgA ,chemistry ,Lactobacillus casei, Multistrain probiotics, Healthy humans, NK cells, Secretory IgA, PCR-DGGE ,Bacteria ,Food Science - Abstract
This study compared the effect of two functional fermented milks with simple and complex probiotic mixtures on the microbiota and some immune system functions of healthy adults and children. The complex yogurt, containing Lactobacillus casei and other strains of lactobacilli and bifidobacteria, and the simple yogurt, containing only L. casei, were administered daily to healthy volunteers (41 adults and 36 children) for a period of 4 weeks. Microbiological and molecular analyses of faecal samples indicated that the complex product induced a larger increase in the lactic acid bacteria number, compared with the simple product, without significant alterations of the autochthonous species composition. Of the probiotic strains, L. casei showed the best survival rate in faeces. The influences of both products on the immune system were similar, with increase of NK activity and proliferative response to Candida albicans in adults and secretory-IgA in children saliva, indicating that immunomodulating properties were probably supported by common traits in the two products.
- Published
- 2007
18. Probiotics for experimental obesity prevention: focus on strain dependence and viability of composition.
- Author
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Kobyliak N, Falalyeyeva T, Beregova T, and Spivak M
- Subjects
- Animals, Animals, Newborn, Male, Obesity chemically induced, Rats, Rats, Wistar, Sodium Glutamate adverse effects, Disease Models, Animal, Obesity prevention & control, Probiotics therapeutic use
- Abstract
Introduction: a comparative animal study of the efficacy of intermittent short-course administration of lyophilised single-, three-, and live multistrain probiotic on obesity., Methods: We included 70 rats divided into seven groups (n = 10 in each). Rats of group I were intact. Newborn rats of groups II-VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The group III-V received lyophilised monoprobiotics B. animalis VKL, B. animalis VKB, and L. casei IMVB-7280, respectively. Group VI received the mix of these three probiotic strains. Group VII was treated with multiprobiotic "Symbiter", which contains 14 live probiotic strains (Lactobacillus, Bifi-dobacterium, Propionibacterium, Acetobacter genera)., Results: Neonatal treatment with MSG caused stunted growth, which is why, despite the lack of weight gain dynamics and absence of significant food consumption rate and body weight changes at day 120, we noted the development of obesity in all MSG-obesity rats and in up to 20-70% after probiotic administration. Supplementation of probiotic composition, with preference to live strains, led to a significantly lower prevalence of obesity, and reduction of VAT weight and serum lipid levels as compared to single-strain probiotic. In our comparative single-strain analysis a trend towards more pronounced hypolipidaemic effect and VAT weight reduction was observed for lyophilised L. casei IMVB-7280 as compared to B. animalis VKL and VKB strains., Conclusions: Multistrain formed mutualistic interactions in mixtures and therefore able to share with different metabolites, affect differ-ent receptors and produced various of biologically active compounds which synergistic overall effect greater than the sum of the single effects.
- Published
- 2017
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