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Made available in DSpace on 2021-06-25T11:10:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The importance of studying the atmospheric pollution due to its effects on human health and other ecosystems, the inexistence of national production of equipment for air sample collection, and the high cost of the imported equipment (especially in developing countries) led the authors of the present work to construct a low-cost Gent type sampler. The construction of the sampler was carried out by combining low-cost materials with good mechanical strength (such as nylon 6.0), hydraulic piping PVC, and the use of a 3D printer. The innovation of the present work is the employment of a 3D printer using ABS polymer to create the grids that cannot be machined. In addition to the sampler, the system is composed of a vacuum pump, a gas meter, and a rotameter. The total cost of the sampling system amounted at about 1200 USD, and the cost of the manufactured Gent type sampler did not reach 100 USD. The results obtained while using this set for sampling atmospheric aerosol for a period of 11 months were compared with the mass concentration of PM10 obtained from the official environmental company, CETESB of São Paulo State, Brazil, showing good correlation with those from CETESB — which confirmed its effectiveness and suitability for use. The low cost, easy operation, and versatility of the built Gent type sampler enable its use for scientific and academic purposes. The equipment can be useful in environmental monitoring networks, in low-income regions, and as an instrument for environmental education used in universities. Graphical abstract: [Figure not available: see fulltext.]. São Paulo State University (UNESP) Institute of Chemistry Centro de Química Estrutural Faculdade de Ciências Universidade de Lisboa, C8 Campo Grande Instituto Nacional de Pesquisas Espaciais – INPE São Paulo State University (Unesp) Biosciences Languages and Exact Sciences Institute Ibilce São Paulo State University (UNESP) Institute of Chemistry São Paulo State University (Unesp) Biosciences Languages and Exact Sciences Institute Ibilce

Made available in DSpace on 2020-12-12T01:52:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 Air pollution causes deleterious effects on human health with aerosols being among the most polluting agents. The objective of this work is the characterization of the PM2.5 and PM10 aerosol mass in the atmosphere. The methods of analysis include WD-XRF and EDS. Data were correlated with meteorological information and air mass trajectories (model HYSPLIT) by multivariate analysis. A morphological structural analysis was also carried out to identify the probable sources of atmospheric aerosols in the city of São José do Rio Preto, Brazil. The mean mass concentration values obtained were 24.54 μg/m3 for PM10, above the WHO annual standard value of 20 μg/m3 and 10.88 μg/m3 for PM2.5 whose WHO recommended limit is 10 μg/m3. WD-XRF analysis of the samples revealed Si and Al as major components of the coarse fraction. In the fine fraction, the major elements were Al and S. The SEM-FEG characterization allowed identifying the morphology of the particles in agglomerates, ellipsoids and filaments in the PM10, besides spherical in the PM2.5. The analysis by EDS corroborated WD-XRF results, identifying the crustal elements, aluminosilicates and elements of anthropogenic origin in the coarse fraction. For the fine fraction crustal elements were also identified; aluminosilicates, black carbon and spherical particles (C and O) originating from combustion processes were predominant. The use of multivariate analysis to correlate air mass trajectories with the results of the morpho-structural characterization of the particulate matter allowed confirmation of the complex composition of the particles resulting from the combination of both local and long-distance sources. São Paulo State University (Unesp), Chemistry Institute, Analytical Chemistry Department, Araraquara, SP, 14800-060, Brazil; Centro de Química Estrutural - Faculdade de Ciências da Universidade de Lisboa, Edif. C8, Campo Grande, 1749-016 Lisboa, Portugal São Paulo State University (Unesp) Chemistry Institute Analytical Chemistry Department Federal University of São Carlos - UFSCAr Chemistry Department Chemistry and Environmental Sciences Department Biosciences Languages and Exact Sciences Institute Ibilce São Paulo State University (Unesp) São Paulo State University (Unesp), Chemistry Institute, Analytical Chemistry Department, Araraquara, SP, 14800-060, Brazil; Chemistry and Environmental Sciences Department, Biosciences, Languages and Exact Sciences Institute, Ibilce, São Paulo State University (Unesp), São José do Rio Preto, SP, 15054-000, Brazil Centro de Química Estrutural - Faculdade de Ciências da Universidade de Lisboa Edif. C8 São Paulo State University (Unesp) Chemistry Institute Analytical Chemistry Department Chemistry and Environmental Sciences Department Biosciences Languages and Exact Sciences Institute Ibilce São Paulo State University (Unesp)

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:24Z No. of bitstreams: 1 S0100-46702010000400015.pdf: 244002 bytes, checksum: 93026127eaf20554d319bb8064b0ccaf (MD5) Made available in DSpace on 2013-08-22T18:49:24Z (GMT). No. of bitstreams: 1 S0100-46702010000400015.pdf: 244002 bytes, checksum: 93026127eaf20554d319bb8064b0ccaf (MD5) Previous issue date: 2010-01-01 Made available in DSpace on 2013-09-30T19:09:37Z (GMT). No. of bitstreams: 2 S0100-46702010000400015.pdf: 244002 bytes, checksum: 93026127eaf20554d319bb8064b0ccaf (MD5) S0100-46702010000400015.pdf.txt: 22810 bytes, checksum: ba93b1a43cd3db10be4b6533df3db78e (MD5) Previous issue date: 2010-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:19:53Z No. of bitstreams: 2 S0100-46702010000400015.pdf: 244002 bytes, checksum: 93026127eaf20554d319bb8064b0ccaf (MD5) S0100-46702010000400015.pdf.txt: 22810 bytes, checksum: ba93b1a43cd3db10be4b6533df3db78e (MD5) Made available in DSpace on 2014-05-20T14:19:53Z (GMT). No. of bitstreams: 2 S0100-46702010000400015.pdf: 244002 bytes, checksum: 93026127eaf20554d319bb8064b0ccaf (MD5) S0100-46702010000400015.pdf.txt: 22810 bytes, checksum: ba93b1a43cd3db10be4b6533df3db78e (MD5) Previous issue date: 2010-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Este trabalho teve como objetivo otimizar e validar uma metodologia analítica para determinação de 1-hidróxipireno em urina de trabalhadores envolvidos na colheita da cana-de-açúcar. O método utilizado para determinação de 1-hidroxipireno em urina humana utilizado consiste na hidrólise enzimática, extração e clean-up por extração em fase sólida (SPE) e quantificação por cromatografia líquida de alta eficiência com detector de fluorescência (CLAE/Flu). Quatro tipos de cartuchos foram testados para verificação da porcentagem de recuperação. Urina de cortadores de cana-de-açúcar (não-fumantes e de ambos os sexos) foram coletadas no período da safra (n=39) e entressafra (n=34) da cana-de-açúcar. Os melhores resultados de recuperação foram atribuídos aos cartuchos C18. Os mesmos apresentaram recuperação entre 79% e 108%, com coeficiente de variação entre 5% e 10%. O limite de quantificação do método foi de 74 ng de 1-hidroxipireno por litro de urina. A metodologia otimizada e validada foi utilizada para determinação de amostras reais. Os resultados encontrados na urina dos trabalhadores no período da safra variaram de 0,026 a 2,3 μmol de 1-hidroxipireno por mol de creatinina. No período da entressafra os resultados variaram de 0,0023 a 0,38 μmol de 1-hidroxipireno por mol de creatinina. A metodologia validada mostrou-se adequada para determinação de 1-hidroxipireno em urina humana. Os dados obtidos permitem concluir que há forte correlação entre excreção de 1-hidroxipireno e os períodos de safra e entressafra da cana de açúcar. This study aimed to optimize and validate an analytical methodology for determination of 1-hydroxypyrene in urine of workers involved in harvesting sugar cane. The method used for determining 1-hydroxypyrene in human urine is the enzymatic hydrolysis, extraction and clean-up by solid phase extraction (SPE) and quantified by high performance liquid chromatography with fluorescence detection (HPLC / Flu). Four types of cartridges were tested to verify the percentage of recovery. Urine of sugar-cane workers (non-smokers and of both sexes) were collected during the harvest (n = 39) and non-harvest season (n = 34) of cane sugar. The best recovery results were attributed to the C18 cartridges. They presented recovery between 79% and 108%, with a coefficient of variation between 5% and 10%. The limit of quantification was 74 ng of 1-hydroxypyrene per liter of urine. The optimized and validated methodology was used for determination of real samples. The results found in the urine of workers at harvest period ranged from 0.026 to 2.3 μmol of 1-hydroxypyrene per mol creatinine. During the non-harvesting season the results ranged from 0.0023 to 0.38 μmol of 1-hydroxypyrene per mol creatinine. The validated methodology proved suitable for determination of 1-hydroxypyrene in human urine. The data indicate that there is strong correlation between excretion of 1-hydroxypyrene and the periods during and between harvests of sugar cane. Universidade Estadual Paulista Instituto de Química Departamento de Química Analítica Universidade Estadual Paulista Instituto de Química Departamento de Química Orgânica Universidade Estadual Paulista Instituto de Biociências, Letras e Ciências Exatas Laboratório de Epidemiologia Molecular Universidade Estadual Paulista Instituto de Química Departamento de Química Analítica Universidade Estadual Paulista Instituto de Química Departamento de Química Orgânica Universidade Estadual Paulista Instituto de Biociências, Letras e Ciências Exatas Laboratório de Epidemiologia Molecular

Huge amounts of waste containing flame retardants reach landfills annually, which can result in environmental contamination if this type of solid residues is not properly managed. This study presents data concerning the occurrence of organophosphorus flame retardants (OPFRs), polybrominated diphenyl ethers (PBDEs) and new brominated flame retardants (NBFRs) in soil, dust, leachate and well water samples from a landfill in Brazil. Samples were collected in different points of the landfill site, including offices, concierge, electronic waste storage area, bulk waste storage area, a place where a recycling cooperative operates, leachate pound and wells. Most of the flame retardants (FRs) were quantified in soil samples (up to 2500 ng g−1). The tris(2-chloroisopropyl) phosphate (TCIPP) and tris(1,3-dichloroisopropyl) phosphate (TDCIPP) were present at the highest levels in the site where bulk waste was disposed in the open air. The most abundant brominated FRs in soil samples were BDE-99, BDE-209, decabromodiphenyl ethane (DBDPE) and 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), and the highest levels were observed in the samples collected from the electronic waste storage area. Concerning dust samples, the highest levels of brominated FRs were observed in the electronic waste storage area, while the highest levels of OPFRs were observed in the landfill office. TCIPP, TDCIPP and tris(2-choroethyl) phosphate (TCEP) were quantified in the well water sample collected downstream the bulk waste area. Finally, six OPFRs were quantified in leachate at concentrations ranging from 14 to 965 ng L−1. In conclusion, this study demonstrates that an improper management of wastes containing FRs in landfills can potentially contaminate the surrounding environment and groundwater., The authors acknowledges the National Council for Scientific and Technological Development (CNPq – Brazil, grant number 401756/2013-0), the Program Science Without Borders (Brazil, grant number 313856/2013-3), the Foundation for Support of Science, Technology and Education (FACTE, Brazil), and the Spanish Research Council, Spain. This study has been done in the frame of the Spain-Brazil cooperation project COOP-B20361.

Made available in DSpace on 2018-12-11T17:21:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-10 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Among the new technologies developed for the heavy-duty fleet, the use of Selective Catalytic Reduction (SCR) aftertreatment system in standard Diesel engines associated with biodiesel/diesel mixtures is an alternative in use to control the legislated pollutants emission. Nevertheless, there is an absence of knowledge about the synergic behaviour of these devices and biodiesel blends regarding the emissions of unregulated substances as the Polycyclic Aromatic Hydrocarbons (PAHs) and Nitro-PAHs, both recognized for their carcinogenic and mutagenic effects on humans. Therefore, the goal of this study is the quantification of PAHs and Nitro-PAHs present to total particulate matter (PM) emitted from the Euro V engine fuelled with ultra-low sulphur diesel and soybean biodiesel in different percentages, B5 and B20. PM sampling was performed using a Euro V – SCR engine operating in European Stationary Cycle (ESC). The PAHs and Nitro-PAHs were extracted from PM using an Accelerated Solvent Extractor and quantified by GC–MS. The results indicated that the use of SCR and the largest fraction of biodiesel studied may suppress the emission of total PAHs. The Toxic Equivalent (TEQ) was lower when using 20% biodiesel, in comparison with 5% biodiesel on the SCR system, reaffirming the low toxicity emission using higher percentage biodiesel. The data also reveal that use of SCR, on its own, suppress the Nitro-PAHs compounds. In general, the use of larger fractions of biodiesel (B20) coupled with the SCR aftertreatment showed the lowest PAHs and Nitro-PAHs emissions, meaning lower toxicity and, consequently, a potential lower risk to human health. From the emission point of view, the results of this work also demonstrated the viability of the Biodiesel programs, in combination with the SCR systems, which does not require any engine adaptation and is an economical alternative for the countries (Brazil, China, Russia, India) that have not adopted Euro VI emission standards. Environmental Engineering Department Federal University of Paraná Mathematics Department Federal University of Technology Paraná Federal University of São Paulo Institute of Technology for Development Lactec Chemical Engineering Department Federal University of Paraná Analytical Chemistry Department Institute of Chemistry São Paulo State University - UNESP Division of Chemistry and Environmental Science School of Science and the Environment Manchester Metropolitan University Molecular Science Institute School of Chemistry University of the Witwatersrand Analytical Chemistry Department Institute of Chemistry São Paulo State University - UNESP CNPq: 402391/2009-8 CNPq: 558697/2012-0

Made available in DSpace on 2018-12-11T17:15:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Indoor dust is considered an important human exposure route to flame retardants (FRs), which has arised concern due the toxic properties of some of these substances. In this study, ten organophosphorus flame retardants (OPFRs), eight polybrominated diphenyl ethers (PBDEs) and four new brominated flame retardants (NBFRs) were determined in indoor dust from different places in Araraquara-SP (Brazil). The sampled places included houses, apartments, offices, primary schools and cars. The analysis of the sample extracts was performed by gas chromatography coupled to mass spectrometry and two ionization techniques were used (electron ionization – EI; electron capture negative ionization – ECNI). OPFRs were the most abundant compounds and tris(2-butoxyethyl) phosphate (TBOEP), tris(phenyl) phosphate (TPHP), tris(1,3-dichloroisopropyl) phosphate (TDCIPP) and tris(2-chloroisopropyl) phosphate (TCIPP) were present at the highest concentrations. Among the brominated FRs, the most ubiquitous compounds were BDE-209, bis(2-ethylhexyl) tetrabromophthalate (BEH-TEBP) and decabromodiphenyl ethane (DBDPE). Statistical analysis revealed that there were differences among dust typologies for TBOEP, TDCIPP, ethylhexyl diphenyl phosphate (EHDPHP), BDE-209, 2-ethylhexyl 2,3,4,5-tetrabromobenzoate (EH-TBB), BEH-TEBP and DBDPE, which were attributed to different construction materials in each particular environment and to the age of the buildings. The highest levels of brominated FRs were observed in offices, TBOEP was at high concentration in primary schools, and TDCIPP was at high concentration in cars. A preliminary risk assessment revealed that toddlers were exposed to TBOEP levels higher than the reference dose when considering the worst case scenario. The results obtained in this study showed for the first time that although Brazil does not regulate the use of FRs, these substances are present in indoor dust at levels similar to the observed in countries that have strict fire safety standards, and that humans are exposed to complex mixtures of these contaminants via indoor dust. PBDEs, NBFRs and OPFRs were detected in indoor dust from houses, apartments, schools, offices and cars, and a risk associated to TBOEP exposure was estimated for toddlers. Department of Analytical Chemistry Institute of Chemistry UNESP - Univ Estadual Paulista, Professor Francisco Degni 55 Department of Environmental Chemistry IDAEA-CSIC, Jordi Girona 18-26 Department of Analytical Chemistry Institute of Chemistry UNESP - Univ Estadual Paulista, Professor Francisco Degni 55 CNPq: 401756/2013-0

Made available in DSpace on 2018-12-11T16:52:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Submarine sewage outfalls (SSOs) are considered the main input source of contaminants of emerging concern continuously released in coastal areas, with the potential to cause adverse effects for aquatic organisms. This work presents the investigation of nine endocrine disrupting chemicals (EDCs) and 26 pharmaceutically active chemicals (PhACs) in marine sediments within the vicinities of 7 SSOs along the São Paulo State Coast (Brazil). Method optimization for the multi-residue determination by GC–MS/MS and LC-MS/MS using QuEChERS extraction/clean-up are discussed. Results demonstrate the occurrence of EDCs in concentrations ranging from less than method quantification limits (MQL) to 72.5 ng g−1 in sediments. All PhACs were

Made available in DSpace on 2018-12-11T17:12:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 The concomitant exposure to low doses of various pesticides is one of the most relevant issues in human toxicology today. An experimental toxicology study was developed to evaluate the effects of this type of exposure on the reproductive capacity of females of three species of rats that were exposed to mixtures of dicofol, dieldrin, endosulfan and permethrin at low doses (LOAEL and NOAEL). In this context, we have developed a method for determining pesticides in adipose tissue (0.5 g, 49% lipid) associated with the ovaries, based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) strategy. The method quantification limit (LOQ) was 0.5 mg/kg for dicofol and permethrin, 0.05 mg/kg for endosulfan and dieldrin and 0.2 mg/kg for diclorobenzophenone. Mean recoveries ranged from 75% to 93% with a relative standard deviation

Made available in DSpace on 2018-11-26T15:28:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2021-07-15T15:01:00Z : No. of bitstreams: 1 S0103-50532016000100109.pdf: 646578 bytes, checksum: 7262b38086fc440223b7630250af4215 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Instituto Nacional de Ciencia e Tecnologia de Bioanalitica (INCTBio) Fundacao Araucaria - Parana Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) It is well known that selectivity of molecularly imprinted polymers (MIPs) depends on adequate choice of functional monomer before the experimental synthesis. Computational simulation seems to be an ideal way to produce selective MIPs. In this work, we have proposed the use of semi-empirical simulation to obtain the best monomer able to strongly interact with ciprofloxacin. Twenty functional monomers were evaluated through semi-empirical quantum chemistry method and three MIPs were synthesized using the monomers acrylamide (M5), acrylic acid (M4) and 1-vinylimidazole (M16), yielding the maximum adsorption capacities of 282.0, 223.8 and 202.5 mu mol g(-1), respectively, as predicted by the computational simulation. From competitive adsorption studies in the presence of structurally similar compounds, the MIP synthesized with acrylamide was found to possess higher specific selectivity factors (S) if compared to non-imprinted polymer (NIP), thus indicating good recognition selectivity for the ciprofloxacin. Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil Inst Fed Parana, BR-84269090 Telemaco Borba, PR, Brazil Univ Estadual Londrina, Dept Quim, BR-86050482 Londrina, PR, Brazil Univ Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil

Butyltin (BTs) quantification in environmental matrices can be affected by interfering species found primarily in complex matrices, such as sediment and biota tissues. This study investigated matrix effects in analytical procedures for butyltin (TBT, DBT and MBT) quantification and speciation in sediments and in two fish tissues (gill and liver) by gas chromatography with pulsed flame photometric detection (GC-PFPD) analysis. Unlike sediment samples, tissues exhibited a significant matrix effect, thus, the quantification should be made by curve over matrix to avoid quantification errors and loss of analytical accuracy. A quantificação de compostos butílicos de estanho (BTs) em matrizes ambientais pode ser afetada pela ocorrência de interferentes encontrados em matrizes complexas tais como sedimentos e tecidos biológicos. O presente estudo investigou o efeito matriz em procedimentos analíticos para quantificação e especiação de BTs (TBT, DBT e MBT) em sedimentos e em dois tecidos (brânquias e fígado) de peixe, utilizando a técnica de cromatografia a gás com detecção fotométrica de chama pulsante (GC-PFPD). Diferentemente dos sedimentos avaliados, um efeito matriz significativo foi observado para os tecidos de peixe investigados, indicando que a quantificação de BTs deve ser realizada através da curva construída na matriz para evitar, dessa forma, erros de quantificação e redução da precisão analítica.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:43:24Z No. of bitstreams: 1 S1677-04202012000300009.pdf: 322083 bytes, checksum: f5c6acb1bbcc9325f1c64bef32a8d97e (MD5) Made available in DSpace on 2013-08-22T18:43:24Z (GMT). No. of bitstreams: 1 S1677-04202012000300009.pdf: 322083 bytes, checksum: f5c6acb1bbcc9325f1c64bef32a8d97e (MD5) Previous issue date: 2012-09-01 Made available in DSpace on 2013-09-30T19:31:03Z (GMT). No. of bitstreams: 2 S1677-04202012000300009.pdf: 322083 bytes, checksum: f5c6acb1bbcc9325f1c64bef32a8d97e (MD5) S1677-04202012000300009.pdf.txt: 39386 bytes, checksum: c4fd5fa51ed1a8b8de3a529fb6cbfcc1 (MD5) Previous issue date: 2012-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:08:31Z No. of bitstreams: 2 S1677-04202012000300009.pdf: 322083 bytes, checksum: f5c6acb1bbcc9325f1c64bef32a8d97e (MD5) S1677-04202012000300009.pdf.txt: 39386 bytes, checksum: c4fd5fa51ed1a8b8de3a529fb6cbfcc1 (MD5) Made available in DSpace on 2014-05-20T15:08:31Z (GMT). No. of bitstreams: 2 S1677-04202012000300009.pdf: 322083 bytes, checksum: f5c6acb1bbcc9325f1c64bef32a8d97e (MD5) S1677-04202012000300009.pdf.txt: 39386 bytes, checksum: c4fd5fa51ed1a8b8de3a529fb6cbfcc1 (MD5) Previous issue date: 2012-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Phytohormone analysis using liquid chromatography combined with tandem mass spectrometry is a very important tool for studies involving plant metabolism; however, this analysis can be affected by matrix composition. Although it is necessary to understand this effect to produce reliable results, it has been widely neglected in analyses of plant hormones. Leaf extracts from Bauhinia variegata were obtained by solvent extraction followed by solid-phase cleanup. The extract was analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) in negative ionization mode, using the multiple reaction monitoring mode with two or three transitions to enhance analytical quality control. Although deuterated standards were used as surrogates, pronounced matrix effects were detected for phaseic acid (PA), abscisic acid (ABA) -glycosyl ester (ABA-GE) and dihydrophaseic acid (DPA), whereas ABA, neoPA and 7'-hydroxy-ABA (7'OHABA) showed negligible matrix effects. The method was validated using spiked samples and was applied to monitor ABA, PA, DPA, neoPA, 7´OHABA and ABA-GE on a daily basis. Analyte recoveries from spiked samples ranged from 67% to 87%. The highest leaf concentration of phytohormones was found at 2:00 pm and 5:00 pm, which represent typical day times related to the decrease of stomatal conductance. Despite the use of deuterated phytohormones as internal standards, we showed that the use of a calibration curve constructed with a matrix extract is mandatory for the reliable quantification of ABA and its metabolites, especially PA, ABA-GE and DPA. Daily variations in endogenous ABA leaf concentration were discussed. Universidade Estadual Paulista IB Departamento de Botânica Universidade Estadual Paulista IQ Departamento de Química Analítica Universidade Estadual Paulista IB Departamento de Botânica Universidade Estadual Paulista IQ Departamento de Química Analítica

Made available in DSpace on 2013-08-12T18:39:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T19:08:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:59:51Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:59:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Apoio à Ciência Tecnologia e Educação (FACTE) The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas chromatography with pulsed flame photometric detector (GC-PFPD) after extraction with an apolar solvent (toluene) and Grignard derivatization. The limits of detection of the method (LOD) were 2.0 and 2.8 ng g(-1) for TBT and DBT (dibutyltin), respectively, and its limits of quantification (LOQ) were 6.6 and 8.9 ng g(-1) for TBT and DBT, respectively. The method was applied to samples from Santos Estuary, São Paulo State, Brazil. TBT and DBT concentrations ranged from 26.7 to 175.0 ng g(-1) and from 46.2 to 156.0 ng g(-1), respectively. These concentrations are worrisome since toxic effects (such as endocrine disruption) have been reported for other organisms even under lower levels of registred at this study. São Paulo State Univ UNESP, Dept Analyt Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil Univ São Paulo, Dept Chem & Geol Oceanog, Oceanog Inst, BR-05508900 São Paulo, Brazil São Paulo State Univ UNESP, Dept Zool, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil Univ São Paulo, Dept Biol Oceanog, Oceanog Inst, BR-05508120 São Paulo, Brazil São Paulo State Univ UNESP, Dept Analyt Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil São Paulo State Univ UNESP, Dept Zool, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil FAPESP: 06/57007-3 FAPESP: 06/61589-8 CNPq: 301240/2006-0

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:22Z No. of bitstreams: 1 S0100-46702010000100003.pdf: 587765 bytes, checksum: 2907cdaf2ee7ed4a014694af1bb3a848 (MD5) Made available in DSpace on 2013-08-22T18:49:22Z (GMT). No. of bitstreams: 1 S0100-46702010000100003.pdf: 587765 bytes, checksum: 2907cdaf2ee7ed4a014694af1bb3a848 (MD5) Previous issue date: 2010-01-01 Made available in DSpace on 2013-09-30T19:39:46Z (GMT). No. of bitstreams: 2 S0100-46702010000100003.pdf: 587765 bytes, checksum: 2907cdaf2ee7ed4a014694af1bb3a848 (MD5) S0100-46702010000100003.pdf.txt: 38613 bytes, checksum: c760928a5da69a037aaf860bc803473a (MD5) Previous issue date: 2010-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:10:35Z No. of bitstreams: 2 S0100-46702010000100003.pdf: 587765 bytes, checksum: 2907cdaf2ee7ed4a014694af1bb3a848 (MD5) S0100-46702010000100003.pdf.txt: 38613 bytes, checksum: c760928a5da69a037aaf860bc803473a (MD5) Made available in DSpace on 2014-05-20T15:10:35Z (GMT). No. of bitstreams: 2 S0100-46702010000100003.pdf: 587765 bytes, checksum: 2907cdaf2ee7ed4a014694af1bb3a848 (MD5) S0100-46702010000100003.pdf.txt: 38613 bytes, checksum: c760928a5da69a037aaf860bc803473a (MD5) Previous issue date: 2010-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Laboratórios e centros de pesquisa consomem uma grande variedade de substâncias químicas perigosas e, consequentemente, geram resíduos contendo-as, como por exemplo: resíduo de formaldeído. O formol é bastante empregado na conservação de peças anatômicas, gerando muitos litros desse resíduo por ano. O presente trabalho sugere o uso do processo foto-Fenton com irradiação solar para tratar resíduo contendo formaldeído. Alguns aspectos foram estudados como a concentração da fonte de ferro, da amostra e do peróxido de hidrogênio, além da aplicação de agitação magnética. O uso de ferrioxilato (0,5 mol L-1) mostrou ser mais eficiente na degradação da matéria orgânica quando comparado ao nitrato férrico. Já a concentração de peróxido necessária para tratar 500 mg C L-1 foi de 1 mol L-1. Sob essas condições todo formaldeído detectável foi degradado e foi possível remover 89% da matéria orgânica dissolvida na amostra em duas horas. Tais resultados são considerados satisfatórios para descartar na rede de esgoto, segundo a agência paulista de controle ambiental. Laboratories consume great amounts of hazardous chemicals substances and consequently generate wastewater containing them, for example formaldehyde. This substance is widely utilized to preserve biological samples generating many liters of this residue every year. The present work proposes the use of the photo-Fenton process to treat formaldehyde wastewater using sunlight irradiation. Some aspects were investigated such as the iron source, sample and hydrogen peroxide concentration and also the use of stirred systems. The use of ferrioxalate (0.5 mmol L-1) improved the efficiency of the process in relation to the use of iron nitrate, while at least 1.0 mol L-1 H2O2 is necessary to treat the sample of the 500 mg C L-1. Under these conditions, every formaldehyde detectable was degradeted and 89% of the dissolved organic carbon was removed in two hours of exposure to sunlight. These results are satisfaction considerate for São Paulo State Environmental Agency. UNESP Waste Management Program UNESP Institute of Chemistry UNESP Waste Management Program UNESP Institute of Chemistry

Ethanolic extract of Casearia sylvestris is thought to be antimutagenic. In this study, we attempted to determine whether this extract and casearin X (a clerodane diterpene from C. sylvestris) are protective against the harmful effects of airborne pollutants from sugarcane burning. To that end, we used the Tradescantia micronucleus test in meiotic pollen cells of Tradescantia pallida, the micronucleus test in mouse bone marrow cells, and the comet assay in mouse blood cells. The mutagenic compound was total suspended particulate (TSP) from air. For the Tradescantia micronucleus test, T. pallida cuttings were treated with the extract at 0.13, 0.25, or 0.50mg/ml. Subsequently, TSP was added at 0.3mg/ml, and tetrads from the inflorescences were examined for micronuclei. For the micronucleus test in mouse bone marrow cells and the comet assay in mouse blood cells, Balb/c mice were treated for 15days with the extract—3.9, 7.5, or 15.0mg/kg body weight (BW)—or with casearin X—0.3, 0.25, or 1.2mg/kg BW—after which they received TSP (3.75mg/kg BW). In T. pallida and mouse bone marrow cells, the extract was antimutagenic at all concentrations tested. In mouse blood cells, the extract was antigenotoxic at all concentrations, whereas casearin X was not antimutagenic but was antigenotoxic at all concentrations. We conclude that C. sylvestris ethanolic extract and casearin X protect DNA from damage induced by airborne pollutants from sugarcane burning.