Tributyltin in crustacean tissues: analytical performance and validation of method

Made available in DSpace on 2013-08-12T18:39:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T19:08:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:59:51Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:59:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Apoio à Ciência Tecnologia e Educação (FACTE) The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas chromatography with pulsed flame photometric detector (GC-PFPD) after extraction with an apolar solvent (toluene) and Grignard derivatization. The limits of detection of the method (LOD) were 2.0 and 2.8 ng g(-1) for TBT and DBT (dibutyltin), respectively, and its limits of quantification (LOQ) were 6.6 and 8.9 ng g(-1) for TBT and DBT, respectively. The method was applied to samples from Santos Estuary, São Paulo State, Brazil. TBT and DBT concentrations ranged from 26.7 to 175.0 ng g(-1) and from 46.2 to 156.0 ng g(-1), respectively. These concentrations are worrisome since toxic effects (such as endocrine disruption) have been reported for other organisms even under lower levels of registred at this study. São Paulo State Univ UNESP, Dept Analyt Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil Univ São Paulo, Dept Chem & Geol Oceanog, Oceanog Inst, BR-05508900 São Paulo, Brazil São Paulo State Univ UNESP, Dept Zool, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil Univ São Paulo, Dept Biol Oceanog, Oceanog Inst, BR-05508120 São Paulo, Brazil São Paulo State Univ UNESP, Dept Analyt Chem, Inst Chem, BR-14800900 Araraquara, SP, Brazil São Paulo State Univ UNESP, Dept Zool, Inst Biosci, BR-13506900 Rio Claro, SP, Brazil FAPESP: 06/57007-3 FAPESP: 06/61589-8 CNPq: 301240/2006-0