15 results on '"Leite, Gleice"'
Search Results
2. Comparison of methods for the detection of in vitro synergy in multidrug-resistant gram-negative bacteria
- Author
-
Gaudereto, Juliana Januario, Neto, Lauro Vieira Perdigão, Leite, Gleice Cristina, Espinoza, Evelyn Patricia Sanchez, Martins, Roberta Cristina Ruedas, Villas Boa Prado, Gladys, Rossi, Flavia, Guimarães, Thais, Levin, Anna Sara, and Costa, Silvia Figueiredo
- Published
- 2020
- Full Text
- View/download PDF
3. Alternative drugs against multiresistant Gram-negative bacteria
- Author
-
Perdigão Neto, Lauro Vieira, Oliveira, Maura Salaroli, Orsi, Tatiana D’Annibale, Prado, Gladys Villas Boas do, Martins, Roberta Cristina Ruedas, Leite, Gleice Cristina, Marchi, Ana Paula, Lira, Esther Sant’Ana de, Côrtes, Marina Farrel, Espinoza, Evelyn Patricia Sanchez, Carrilho, Cláudia Maria Dantas de Maio, Boszczowski, Ícaro, Guimarães, Thais, Costa, Silvia Figueiredo, and Levin, Anna S.
- Published
- 2020
- Full Text
- View/download PDF
4. Genetic and virulence characterization of colistin-resistant and colistin-sensitive A. baumannii clinical isolates
- Author
-
Leite, Gleice Cristina, Stabler, Richard A., Neves, Patrícia, Perdigão Neto, Lauro V., Ruedas Martins, Roberta C., Rizek, Camila, Rossi, Flavia, Levin, Anna S., and Costa, Silvia Figueiredo
- Published
- 2019
- Full Text
- View/download PDF
5. In vitro synergy of β-lactam combinations against KPC-producing Klebsiella pneumoniae strains.
- Author
-
Lawandi, Alexander, Leite, Gleice, Cheng, Matthew P, Lefebvre, Brigitte, Longtin, Jean, and Lee, Todd C
- Subjects
- *
MEROPENEM , *KLEBSIELLA pneumoniae , *CEFTAZIDIME , *CARBAPENEMS , *IMIPENEM , *ENTEROBACTERIACEAE - Abstract
Background: Double carbapenem therapy has been promoted as an alternative treatment for infections due to carbapenemase-producing Enterobacteriaceae where carbapenemase inhibitors are unavailable or when other agents have demonstrated toxicity with equally limited evidence. The capacity of other β-lactams and β-lactamase inhibitors to provide synergistic activity with carbapenems is unclear.Objectives: This study sought to investigate the in vitro synergistic potential of other β-lactam/β-lactamase combinations with meropenem against KPC producers.Methods: Time-kill assays were performed on 24 unique strains of KPC-producing Klebsiella pneumoniae. Combinations evaluated included meropenem or imipenem with one of the following: ertapenem, piperacillin/tazobactam or ceftolozane/tazobactam. Concentrations used for each drug were those considered physiologically attainable in patients with a time above the concentration exceeding 40%-50% of the dose interval. Combinations were considered to be synergistic when they reduced bacterial cfu/mL by ≥2 log10 at 24 h as compared with the single most active agent.Results: The combination of piperacillin/tazobactam with meropenem was found to be synergistic against 70.8% of the isolates, followed by ertapenem with meropenem (58.3%) and ceftolozane/tazobactam with meropenem (41.7%). The piperacillin/tazobactam combination was found to be more bactericidal than the other combinations, with 58.3% of isolates demonstrating a ≥4 log10 cfu/mL reduction at 24 h, as compared with 37.5% for ertapenem and 20.8% for ceftolozane/tazobactam combinations.Conclusions: The combination of piperacillin/tazobactam with meropenem may be a potential therapy against KPC-producing K. pneumoniae when other therapies are unavailable or prohibitively toxic. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
6. Antimicrobial Combinations against Pan-Resistant Acinetobacter baumannii Isolates with Different Resistance Mechanisms.
- Author
-
Leite, Gleice Cristina, Oliveira, Maura Salaroli, Perdigão-Neto, Lauro Vieira, Rocha, Cristiana Kamia Dias, Guimarães, Thais, Rizek, Camila, Levin, Anna Sara, and Costa, Silvia Figueiredo
- Subjects
- *
ACINETOBACTER baumannii , *ANTI-infective agents , *VANCOMYCIN resistance , *MEMBRANE proteins , *COLISTIN - Abstract
The study investigated the effect of antibiotic combinations against 20 clinical isolates of A. baumannii (seven colistin-resistant and 13 colistin-susceptible) with different resistance mechanisms. Clinical data, treatment, and patient mortality were evaluated. The following methods were used: MIC, PCRs, and outer membrane protein (OMP) analysis. Synergy was investigated using the checkerboard and time-kill methods. Clonality was evaluated by PFGE. Based on clonality, the whole genome sequence of six A. baumannii isolates was analyzed. All isolates were resistant to meropenem, rifampicin, and fosfomycin. OXA-23 and OXA-143 were the most frequent carbapenemases found. Four isolates showed loss of a 43kDa OMP. The colistin-susceptible isolates belonged to different clones and showed the highest synergistic effect with fosfomycin-amikacin. Among colistin-resistant isolates, the highest synergistic effect was observed with the combinations of colistin-rifampicin followed by colistin-vancomycin. All colistin-resistant isolates harbored blaOXA-23-like and belonged to CC113. Clinical and demographic data were available for 18 of 20 patients. Fourteen received treatment and eight patients died during treatment. The most frequent site of infection was the blood in 13 of 14 patients. Seven patients received vancomycin plus an active drug against A. baumannii; however, mortality did not differ in this group. The synergistic effect was similar for colistin-susceptible isolates of distinct clonal origin presenting with the same resistance mechanism. Overall mortality and death during treatment was high, and despite the high synergism in vitro with vancomycin, death did not differ comparing the use or not of vancomycin plus an active drug against A. baumannii. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
7. Characterization of carbapenem-resistant Pseudomonas aeruginosa clinical isolates, carrying multiple genes coding for this antibiotic resistance.
- Author
-
Rizek, Camila, Liang Fu, dos Santos, Leticia Cavalcanti, Leite, Gleice, Ramos, Jessica, Rossi, Flavia, Guimaraes, Thais, Levin, Anna S., and Costa, Silvia Figueiredo
- Subjects
PSEUDOMONAS aeruginosa ,GENES ,CARBAPENEMS ,DRUG resistance ,ANTIBIOTICS - Abstract
Background Carbapenemase genes are one of the most frequent mechanisms reported in carbapenemresistant P. aeruginosa; however, description of P. aeruginosa co-harbouring two or more carbapenemases is unusual. Methods In this study we evaluated the presence of carbapenemase genes and the clonality of P. aeruginosa isolates obtained from a hospital over a 12-year period. A total of 127 isolates of carbapenem-resistant P. aeruginosa recovered from 109 patients feces (four samples), rectal swab (three samples), nasal swab (one sample) and anal abscess (one sample), were evaluated. Minimum inhibitory concentrations of the following antibiotics imipenem, meropenem and polymyxin E were determined by broth microdilution. The molecular profile of isolates was evaluated by pulsed field gel electrophoresis (PFGE). PCR for the following carbapenemase genes bla
IMP ; blaSPM ; blaVIM ; blaSIM ; blaNDM ; blaKPC ; blaGES and nucleotide sequencing to confirm the enzyme gene types were performed and compared with the database available on the Internet (BLAST-http://www.ncbi.nlm.nhi.gov/blast/). Results All isolates were carbapenem-resistant, their MIC50 and MIC90 were respectively 64 μg/mL and 256 μg/mL to imipenem and 32 μg/mL and 256 μg/mL to meropenem, all isolates except one (MIC = 8 mg/L) were susceptible to polymyxin E. The most frequent carbapenemase genes identified were blaSPM identified in 41 isolates (32%), followed by 10 with blaKpc and 5 with blaVIM (3.9%). All belonged to the class SPM-1 and VIM-2. In 2011, one isolate harbouring three carbapenemase genes (SPM-1, VIM-2 and KPC-2) that belonged to a new clone was identified in a hematopoietic stem cell transplanted patient. Then, 19 carbapenemresistant P. aeruginosa were identified in an outbreak that occurred in the bone marrow transplant unit, all positive for SPM-1 gene, and 9 (47.3%) harbored both SPM-1 and KPC. Conclusion Our findings showed that PCR for KPC gene should be performed to evaluate carbapenem resistance in P. aeruginosa and that this agent can harbor more than one carbapenemase gene. Attention should be focused on the possible rapid spread of KPC in P. aeruginosa isolates and for the fact that P. aeruginosa may become a reservoir of this transmissible resistance mechanism. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
8. Methicillin-resistant Staphylococcus aureus DNA electrophoretic pattern: temporal changes in an endemic hospital environment.
- Author
-
Leite, Gleice Cristina, Padoveze, Maria Clara, and Moretti, Maria Luiza
- Subjects
- *
DNA , *PULSED-field gel electrophoresis , *METHICILLIN-resistant staphylococcus aureus , *HOSPITAL patients - Abstract
Objective. To describe the analysis of geographical and temporal distribution of DNA profiles determined by pulsed-field gel electrophoresis (PFGE) of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients in a tertiary care university hospital in Brazil. Methods. Ninety-nine samples of MRSA obtained from 89 patients in the period 1999-2004 were studied. MRSA strains were isolated from central venous catheters (33 isolates) and bloodstream infections (66 strains). PFGE with 20 units of SmaI restriction endonuclease was used for genomic typing. Results. Analysis of DNA PFGE of 99 strains of MRSA revealed 26 profiles and their respective related profiles. The mean time interval for detecting MRSA infection was 26 days from hospital admission. Forty-nine patients (57.6%) had a recent hospitalization. The DNA PFGE MRSA profiles were distributed in three clonal groups--I, II, and III--according to the period of time when the MRSA strains were isolated. DNA PFGE MRSA profiles were spread homogeneously through all hospital wards. Conclusions. Changes in the distribution of DNA PFGE MRSA profiles were largely temporal, with clonal groups being replaced over time, without predominance in any hospital ward or any specific area of the hospital. [ABSTRACT FROM AUTHOR]
- Published
- 2011
9. Genetic factors involved in fosfomycin resistance of multidrug-resistant Acinetobacter baumannii.
- Author
-
Leite, Gleice C., Perdigão-Neto, Lauro V., Ruedas Martins, Roberta C., Rizek, Camila, Levin, Anna Sara, and Costa, Silvia F.
- Subjects
- *
ACINETOBACTER baumannii , *FOSFOMYCIN , *ADENYLATE cyclase - Abstract
The treatment of infections caused by A. baumannii is a challenge and fosfomycin has been used as a combination therapy. Moreover, data regarding the fosfomycin resistance mechanism is scarce. The goals of this study were to evaluate fosfomycin susceptibility in polyclonal multi-resistant A. baumannii isolates and characterize the fosfomycin resistance. We analyzed 32 A. baumannii isolates from a Brazilian bacterial collection, followed by their minimum inhibitory concentration (MIC), and whole-genome sequence to detect fosfomycin resistance genes. The isolates showed a fosfomycin MIC ranging from 32 to ≥256 mg/L. All isolates were negative for fosA and fosB genes, and four isolates carried the fosX gene. Two different metabolic pathways that form peptidoglycan precursors were identified. Mutations were observed in the adenylate cyclase gene. All A. baumannii isolates studied showed Val132Ala substitutions in MurA. The analysis showed different ways that may lead to the intrinsic fosfomycin-resistance of A. baumannii, such as alterations on the glycerol-3-phosphate transporter system caused by adenylate cyclase mutations; and a possible connection of cell wall recycling by different metabolic pathways. • This study characterizes the genetic basis of fosfomycin resistance mechanisms in polyclonal multi-resistant clinical Acinetobacter baumannii isolates. • The analysis showed different ways that lead to the intrinsic fosfomycin-resistance of A. baumannii as alterations on the GlpT and a possible connection of cell wall recycling by different metabolic pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
10. Stability of Biological Activity of Frozen β-lactams over Time as Assessed by Time-Lapsed Broth Microdilutions.
- Author
-
Leite, Gleice, Lawandi, Alexander, Cheng, Matthew P., and Lee, Todd
- Subjects
ANTI-infective agents ,CEFTAZIDIME ,CEFTRIAXONE ,IMIPENEM - Abstract
To evaluate the antimicrobial agent's stability stored at −80 °C, six β-lactams (meropenem, ertapenem, imipenem, ceftriaxone, ceftazidime, and piperacillin-tazobactam) were studied using the broth microdilution (BMD). The minimum inhibitory concentration (MIC) remained accurate with the same amount of frozen drug for at least six months. Thereafter, there was a diminishing drug concentration due to instability. At this temperature, most β-lactams can be frozen as a stock concentration for up to six months without a significant loss in antibiotic activity. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
11. Assessment of Piperacillin-Tazobactam-Meropenem Synergy against Serine Carbapenemase-Producing Enterobacterales Using Time-Kill Assays.
- Author
-
Lawandi A, Leite GC, Cheng MP, Lefebvre B, Longtin J, and Lee TC
- Subjects
- Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Humans, Klebsiella pneumoniae, Meropenem pharmacology, Microbial Sensitivity Tests, Piperacillin, Tazobactam Drug Combination, Serine, beta-Lactamases genetics
- Abstract
Synergy between piperacillin-tazobactam and meropenem against KPC-producing Klebsiella pneumoniae was recently demonstrated. We sought to test the combination against a broader range of serine carbapenemase producers. We tested the combination against 10 KPC-producing Escherichia coli and 10 OXA-48 family-producing K. pneumoniae isolates. Antibiotic concentrations used are achievable in critically ill patients. The combination was synergistic against 7 of 10 KPC producers and 9 of 10 OXA-48 producers. There was no synergy detected in control isolates producing NDM-1., (Copyright © 2021 American Society for Microbiology.)
- Published
- 2021
- Full Text
- View/download PDF
12. Synergistic Effect of Ceftazidime-Avibactam with Meropenem against Panresistant, Carbapenemase-Harboring Acinetobacter baumannii and Serratia marcescens Investigated Using Time-Kill and Disk Approximation Assays.
- Author
-
Gaudereto JJ, Perdigão Neto LV, Leite GC, Ruedas Martins R, Boas do Prado GV, Rossi F, Guimarães T, Levin AS, and Costa SF
- Subjects
- Acinetobacter baumannii drug effects, Bacterial Proteins genetics, Drug Combinations, Microbial Sensitivity Tests, Serratia marcescens drug effects, beta-Lactamases genetics, Anti-Bacterial Agents pharmacology, Azabicyclo Compounds pharmacology, Bacterial Proteins metabolism, Ceftazidime pharmacology, Meropenem pharmacology, beta-Lactamases metabolism
- Abstract
Susceptibility of ceftazidime-avibactam and in vitro synergy with meropenem were investigated using disk approximation and time-kill assays against 11 multiresistant Acinetobacter baumannii isolates harboring oxacillinases and 5 Serratia marcescens isolates carrying bla
KPC-2 Ceftazidime-avibactam was very active and synergistic with meropenem against multiresistant S. marcescens isolates. On the other hand, only the A. baumannii isolates coharboring blaOXA-23 and blaOXA-117 displayed synergy. The disk approximation technique presented good sensitivity for synergism in S. marcescens infection., (Copyright © 2019 American Society for Microbiology.)- Published
- 2019
- Full Text
- View/download PDF
13. Carbapenem-resistant Pseudomonas aeruginosa carrying bla VIM-36 assigned to ST308: Indicated non-virulence in a Galleria mellonella model.
- Author
-
Neves PR, Perdigão Neto LV, Ruedas Martins RC, Ramos JF, Leite G, Rossi F, Sanabani SS, Rocha V, Batista MV, Guimaraes T, Levin AS, and Costa SF
- Subjects
- Animals, Bacterial Typing Techniques, DNA, Bacterial genetics, Disease Models, Animal, Larva microbiology, Microbial Sensitivity Tests, Multilocus Sequence Typing, Pseudomonas Infections, Pseudomonas aeruginosa classification, Virulence, Anti-Bacterial Agents pharmacology, Carbapenems pharmacology, Moths microbiology, Pseudomonas aeruginosa drug effects, Pseudomonas aeruginosa genetics, beta-Lactamases genetics
- Abstract
Objectives: Based on pulsed-field gel electrophoresis (PFGE) profile, whole-genome sequencing (WGS) of eight carbapenem-resistant Pseudomonas aeruginosa isolates from a bone marrow transplant unit in São Paulo, Brazil, was performed to investigate the presence of resistance and virulence genes as well as to determine the sequence type (ST) by multilocus sequence typing (MLST)., Methods: The initial phenotypic susceptibility pattern of the isolates was determined by VITEK
® 2. Minimum inhibitory concentrations (MICs) were determined by the broth microdilution method for amikacin, meropenem and colistin. WGS was performed using an Illumina MiSeq system. A Galleria mellonella infection model was used to evaluate the virulence of the strains., Results: WGS demonstrated that mutations in genes encoding outer membrane proteins and efflux pumps in an isolate harbouring blaVIM-36 (ST308) differed from those in isolates harbouring blaSPM (ST277). The mexT gene harboured a mutation resulting in a frameshift in all isolates; in addition, the oprD gene of the blaVIM-36 -carrying isolate had an insertion leading to a frameshift. Virulence genes did not differ between ST277 and ST308 strains. Moreover, only two isolates harbouring blaSPM showed virulence in the G. mellonella model, killing 100% of larvae after 18-24h., Conclusions: P. aeruginosa carrying blaVIM-36 belonging to ST308 was identified for the first time in our hospital. Although the virulence gene profiles were similar in isolates carrying blaSPM and the isolate carrying blaVIM-36 , only two isolates harbouring blaSPM showed virulence in the G. mellonella model., (Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.)- Published
- 2019
- Full Text
- View/download PDF
14. High mortality of bloodstream infection outbreak caused by carbapenem-resistant P. aeruginosa producing SPM-1 in a bone marrow transplant unit.
- Author
-
Chaves L, Tomich LM, Salomão M, Leite GC, Ramos J, Martins RR, Rizek C, Neves P, Batista MV, Amigo U, Guimaraes T, Levin AS, and Costa SF
- Subjects
- Adult, Anti-Bacterial Agents pharmacology, Bacterial Adhesion drug effects, Bacterial Proteins genetics, Bacterial Proteins metabolism, Biofilms drug effects, Brazil epidemiology, Carbapenems pharmacology, Case-Control Studies, Female, Genome-Wide Association Study, Humans, Length of Stay, Male, Middle Aged, Pseudomonas Infections blood, Pseudomonas aeruginosa drug effects, Risk Factors, beta-Lactamases genetics, beta-Lactamases metabolism, Bacteremia mortality, Bone Marrow Transplantation mortality, Disease Outbreaks, Drug Resistance, Multiple, Bacterial, Pseudomonas Infections mortality, Pseudomonas aeruginosa genetics
- Abstract
Purpose: Carbapenem resistance in P. aeruginosa is increasing worldwide. In Brazil, SPM-1 is the main P. aeruginosa carbapenemase identified. Little is known about the virulence factor in SPM-1 clones.Methodolgy. We describe a carbapenem-resistant P. aeruginosa bloodstream infection (CRPa-BSI) outbreak in a bone marrow transplant Unit (BMT). Twenty-nine CRPa-BSI cases were compared to 58 controls. Microbiological characteristics of isolates, such as sensitivity, carbapenemase gene PCR for P. aeruginosa, and PFGE are described, as well as the whole-genome sequence (WGS) of three strains.Results/Key findings. The cultures from environmental and healthcare workers were negative. Some isolates harboured KPC and SPM. The WGS showed that the 03 strains belonged to ST277, presented the same mutations in outer membrane protein, efflux pump, and virulence genes such as those involved in adhesion, biofilm, quorum-sensing and the type III secretion system, but differ regarding the carbapenemase profile. A predominant clone-producing SPM harbouring Tn 4371 was identified and showed cross-transmission; no common source was found. Overall mortality rate among cases was 79 %. The first multivariate analysis model showed that neutropenia (P=0.018), GVHD prophylaxis (P=0.016) and prior use of carbapenems (P=0.0089) were associated with CRPa-BSI. However, when MASCC>21 points and platelets were added in the final multivariate analysis, only prior use of carbapenems remained as an independent risk factor for CRPa-BSI (P=0.043)., Conclusions: The predominant clone belonging to ST277 showed high mortality. Carbapenem use was the only risk factor associated with CRPa-BSI. This finding is a wake-up call for the need to improve management in BMT units.
- Published
- 2017
- Full Text
- View/download PDF
15. Methicillin-resistant Staphylococcus aureus DNA electrophoretic pattern: temporal changes in an endemic hospital environment.
- Author
-
Leite GC, Padoveze MC, and Moretti ML
- Subjects
- Adult, Bacteremia epidemiology, Bacteremia microbiology, Brazil epidemiology, Catheter-Related Infections epidemiology, Catheter-Related Infections microbiology, Cross Infection epidemiology, Female, Hospital Units statistics & numerical data, Hospitals, University, Humans, Male, Methicillin-Resistant Staphylococcus aureus classification, Methicillin-Resistant Staphylococcus aureus drug effects, Middle Aged, Phylogeny, Postoperative Complications epidemiology, Postoperative Complications microbiology, Staphylococcal Infections epidemiology, Time Factors, Young Adult, Cross Infection microbiology, DNA, Bacterial analysis, Electrophoresis, Gel, Pulsed-Field, Methicillin-Resistant Staphylococcus aureus genetics, Staphylococcal Infections microbiology
- Abstract
Objective: To describe the analysis of geographical and temporal distribution of DNA profiles determined by pulsed-field gel electrophoresis (PFGE) of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients in a tertiary care university hospital in Brazil., Methods: Ninetynine samples of MRSA obtained from 89 patients in the period 1999-2004 were studied. MRSA strains were isolated from central venous catheters (33 isolates) and bloodstream infections (66 strains). PFGE with 20 units of SmaI restriction endonuclease was used for genomic typing., Results: Analysis of DNA PFGE of 99 strains of MRSA revealed 26 profiles and their respective related profiles. The mean time interval for detecting MRSA infection was 26 days from hospital admission. Forty-nine patients (57.6%) had a recent hospitalization. The DNA PFGE MRSA profiles were distributed in three clonal groups-I, II, and III-according to the period of time when the MRSA strains were isolated. DNA PFGE MRSA profiles were spread homogeneously through all hospital wards., Conclusions: Changes in the distribution of DNA PFGE MRSA profiles were largely temporal, with clonal groups being replaced over time, without predominance in any hospital ward or any specific area of the hospital.
- Published
- 2011
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.