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Carbapenem-resistant Pseudomonas aeruginosa carrying bla VIM-36 assigned to ST308: Indicated non-virulence in a Galleria mellonella model.
- Source :
-
Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2019 Mar; Vol. 16, pp. 92-97. Date of Electronic Publication: 2018 Sep 20. - Publication Year :
- 2019
-
Abstract
- Objectives: Based on pulsed-field gel electrophoresis (PFGE) profile, whole-genome sequencing (WGS) of eight carbapenem-resistant Pseudomonas aeruginosa isolates from a bone marrow transplant unit in São Paulo, Brazil, was performed to investigate the presence of resistance and virulence genes as well as to determine the sequence type (ST) by multilocus sequence typing (MLST).<br />Methods: The initial phenotypic susceptibility pattern of the isolates was determined by VITEK <superscript>®</superscript> 2. Minimum inhibitory concentrations (MICs) were determined by the broth microdilution method for amikacin, meropenem and colistin. WGS was performed using an Illumina MiSeq system. A Galleria mellonella infection model was used to evaluate the virulence of the strains.<br />Results: WGS demonstrated that mutations in genes encoding outer membrane proteins and efflux pumps in an isolate harbouring bla <subscript>VIM-36</subscript> (ST308) differed from those in isolates harbouring bla <subscript>SPM</subscript> (ST277). The mexT gene harboured a mutation resulting in a frameshift in all isolates; in addition, the oprD gene of the bla <subscript>VIM-36</subscript> -carrying isolate had an insertion leading to a frameshift. Virulence genes did not differ between ST277 and ST308 strains. Moreover, only two isolates harbouring bla <subscript>SPM</subscript> showed virulence in the G. mellonella model, killing 100% of larvae after 18-24h.<br />Conclusions: P. aeruginosa carrying bla <subscript>VIM-36</subscript> belonging to ST308 was identified for the first time in our hospital. Although the virulence gene profiles were similar in isolates carrying bla <subscript>SPM</subscript> and the isolate carrying bla <subscript>VIM-36</subscript> , only two isolates harbouring bla <subscript>SPM</subscript> showed virulence in the G. mellonella model.<br /> (Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacterial Typing Techniques
DNA, Bacterial genetics
Disease Models, Animal
Larva microbiology
Microbial Sensitivity Tests
Multilocus Sequence Typing
Pseudomonas Infections
Pseudomonas aeruginosa classification
Virulence
Anti-Bacterial Agents pharmacology
Carbapenems pharmacology
Moths microbiology
Pseudomonas aeruginosa drug effects
Pseudomonas aeruginosa genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2213-7173
- Volume :
- 16
- Database :
- MEDLINE
- Journal :
- Journal of global antimicrobial resistance
- Publication Type :
- Academic Journal
- Accession number :
- 30244038
- Full Text :
- https://doi.org/10.1016/j.jgar.2018.09.007