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21. Exploring the Diversity of Red Microalgae for Exopolysaccharide Production

Author

Borjas Esqueda, Aldo Daniel, Gardarin, Christine, Laroche, Céline, Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), and Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA)

Subjects
Sulfates, Drug Discovery, Polysaccharides, Bacterial, Rhodophyta, Microalgae, exopolysaccharide, Pharmaceutical Science, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, microalgae, rhodophyta, Porphyridium, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Culture Media
Abstract

International audience; Microalgae constitute a remarkable biological diversity but a limited number of them have been the object of study for their ability to produce exoplysaccharides (EPS). Among them, the red marine microalgae Porphyridium or Rhodella produce sulphated EPS, exhibiting some biological activities with potential interest in the pharmaceutical and cosmetic industries. EPS from Porphyridium and Rhodella being relatively similar in their composition, it has long been considered that all the red microalgae produced similar EPS and no attention was paid to other red microalgae. The objective of our work was then to explore the diversity of red microalgae for the production of EPS, focusing in this first step on the screening of the strains for their ability to produce EPS and preliminary structural characterization. The study was conducted with 11 microalgae strains belonging to the proteorhodophytina subphylum. All microalgae were able to produce EPS, released in the culture medium (strains belonging to Porphyridiophyceae and Rhodellophyceae classes) or remaining bound to the cells (strains from Stylonematophyceae class). The analysis of monosaccharides composition was found significantly different, with for instance high levels of glucuronic acids in the EPS from C. japonica and N. cyanea, but also strong differences in the sulphation degrees of polymers (between 1.2 and 28.7% eq. SO4).

Published
2022
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22. Elicitor Activity of Low-Molecular-Weight Alginates Obtained by Oxidative Degradation of Alginates Extracted from Sargassum muticum and Cystoseira myriophylloides.

Author

Aitouguinane, Meriem, El Alaoui-Talibi, Zainab, Rchid, Halima, Fendri, Imen, Abdelkafi, Slim, El-Hadj, Mohamed Didi Ould, Boual, Zakaria, Le Cerf, Didier, Rihouey, Christophe, Gardarin, Christine, Dubessay, Pascal, Michaud, Philippe, Pierre, Guillaume, Delattre, Cédric, and El Modafar, Cherkaoui

Abstract

Alginates extracted from two Moroccan brown seaweeds and their derivatives were investigated for their ability to induce phenolic metabolism in the roots and leaves of tomato seedlings. Sodium alginates (ALSM and ALCM) were extracted from the brown seaweeds Sargassum muticum and Cystoseira myriophylloides, respectively. Low-molecular-weight alginates (OASM and OACM) were obtained after radical hydrolysis of the native alginates. Elicitation was carried out by foliar spraying 20 mL of aqueous solutions (1 g/L) on 45-day-old tomato seedlings. Elicitor capacities were evaluated by monitoring phenylalanine ammonia-lyase (PAL) activity, polyphenols, and lignin production in the roots and leaves after 0, 12, 24, 48, and 72 h of treatment. The molecular weights (Mw) of the different fractions were 202 kDa for ALSM, 76 kDa for ALCM, 19 kDa for OACM, and 3 kDa for OASM. FTIR analysis revealed that the structures of OACM and OASM did not change after oxidative degradation of the native alginates. These molecules showed their differential capacity to induce natural defenses in tomato seedlings by increasing PAL activity and through the accumulation of polyphenol and lignin content in the leaves and roots. The oxidative alginates (OASM and OACM) exhibited an effective induction of the key enzyme of phenolic metabolism (PAL) compared to the alginate polymers (ALSM and ALCM). These results suggest that low-molecular-weight alginates may be good candidates for stimulating the natural defenses of plants. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Description of the Wild Strain Rhizobium rosettiformans DSM26376, Reclassified under Peteryoungia rosettiformans comb.nov., for Producing Glucuronan.

Author

Christophe, Gwendoline, Hou, Xiaoyang, Petit, Emmanuel, Traikia, Mounir, Le Cerf, Didier, Rihouey, Christophe, Gardarin, Christine, Delattre, Cédric, Michaud, Philippe, Pierre, Guillaume, and Dubessay, Pascal

Subjects
RHIZOBIUM, GEL permeation chromatography, NUCLEAR magnetic resonance, MOLECULAR weights, INFRARED spectroscopy, POLYSACCHARIDES, LASER ablation inductively coupled plasma mass spectrometry
Abstract

Glucuronan is a polysaccharide composed of β-(1,4)-linked d-glucuronic acids having intrinsic properties and biological activities recoverable in many fields of application. Currently, the description of Sinorhyzobium meliloti M5N1CS mutant bacterial strain as the sole source of glucuronan makes it relevant to the exploration of new microorganisms producing glucuronan. In this study, the Peteryoungia rosettifformans strain (Rhizobia), was identified as a wild producer of an exopolysaccharide (RhrBR46) related to glucuronan. Structural and biochemical features, using colorimetric assays, Fourier infrared spectroscopy, nuclear magnetic resonance, high pressure size exclusion chromatography coupled to multi-angle light laser scattering, and enzymatic assays allowed the characterization of a polyglucuronic acid, having a molecular mass ( M w ¯ ) of 1.85 × 105 Da, and being partially O-acetylated at C-2 and/or C-3 positions. The concentration of Mg2+ ions in the cultivation medium has been shown to impact the structure of RhrBR46, by reducing drastically its M w ¯ (73%) and increasing its DA (10%). Comparative structural analyses between RhrBR46 and the glucuronan from Sinorhyzobium meliloti M5N1CS strain revealed differences in terms of molecular weight, degree of acetylation (DA), and the distribution of acetylation pattern. These structural divergences of RhrBR46 might contribute to singular properties or biological activities of RhrBR46, offering new perspectives of application. [ABSTRACT FROM AUTHOR]

Published
2023
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24. The Effect of Ultrasound Pretreatment on Pectin Extraction from Watermelon Rind Using Microwave-Assisted Extraction.

Author

Forouhar, Ali, Hamdami, Nasser, Djelveh, Gholamreza, Gardarin, Christine, Pierre, Guillaume, Ursu, Alina Violeta, and Michaud, Philippe

Subjects
PECTINS, INTRINSIC viscosity, GALACTURONIC acid, WATERMELONS, ULTRASONIC effects, SURFACE tension
Abstract

Ultrasound pretreatment and microwave-assisted extraction (MAE) techniques were used to extract pectin from watermelon rind. This work investigated the effect of the power and time of pretreatment on the yield and characteristics of the extracted pectin. Imaging studies showed that ultrasonic pretreatment changed the morphology of the raw powder, leading to a greater porosity, and facilitated the extraction process. The highest extraction yield of pectin was 19.08% (w/w), for 5 min of extraction at 500 W ultrasonic pretreatment. Ultrasonic pretreatment affected the degree of esterification and the galacturonic acid content of pectin. The galacturonic acid content of all samples was higher than 69.0%. Extraction pretreatment reduced the surface tension value of the aqueous solution of pectin and affected the foam capacity of pectin and its monosaccharidic composition. The ultrasonic pretreatment caused significant changes in pectin characteristics, such as intrinsic viscosity, viscosity average, molecular weight, ratio of homogalacturonan to rhamnogalacturonan and the water holding capacity. In addition, kinetic studies showed that a kinetic empirical model adequately describes the effect of ultrasonic pretreatment on foam stability and its half-time. [ABSTRACT FROM AUTHOR]

Published
2023
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25. Biochemical Characterization of a Bifunctional Enzyme Constructed by the Fusion of a Glucuronan Lyase and a Chitinase from Trichoderma sp

Author

Baklouti, Zeineb, Delattre, Cédric, Pierre, Guillaume, Gardarin, Christine, Abdelkafi, Slim, Michaud, Philippe, Dubessay, Pascal, Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Département Génie Biologique, Université de Sfax, Unité de Biotechnologie des Algues, Ecole National d’Ingénieurs de Sfax, 3018 Sfax, Institut Universitaire de France (IUF), and Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)

Subjects
bifunctional enzyme, chitinase, polysaccharide, glucuronan lyase, lcsh:Q, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, enzymatic efficiency, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], lcsh:Science
Abstract

International audience; Bifunctional enzymes created by the fusion of a glucuronan lyase (TrGL) and a chitinase (ThCHIT42) from Trichoderma sp. have been constructed with the aim to validate a proof of concept regarding the potential of the chimera lyase/hydrolase by analyzing the functionality and the efficiency of the chimeric constructions compared to parental enzymes. All the chimeric enzymes, including or nor linker (GGGGS), were shown functional with activities equivalent or higher to native enzymes. The velocity of glucuronan lyase was considerably increased for chimeras, and may involved structural modifications at the active site. The fusion has induced a slightly decrease of the thermostability of glucuronan lyase, without modifying its catalytic activity regarding pH variations ranging from 5 to 8. The biochemical properties of chitinase seemed to be more disparate between the different fusion constructions suggesting an impact of the linkers or structural interactions with the linked glucuronan lyase. The chimeric enzymes displayed a decreased stability to temperature and pH variations, compared to parental one. Overall, TrGL-ThCHIT42 offered the better compromise in terms of biochemical stability and enhanced activity, and could be a promising candidate for further experiments in the field of fungi Cell Wall-Degrading Enzymes (CWDEs)

Published
2020
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26. Research on the origin and the side effects of chitosan stabilizing properties in wine

Author

Dols, Marguerite, Paulin, Margot, Miot-Sertier, Cecile, Claisse, Olivier, Ballestra, Patricia, Albertin, Warren, Marchal, Axel, Masneuf, Isabelle, Delattre, Cédric, Pierre, Guillaume, Dubessay, Pascal, Gardarin, Christine, Michaud, Philippe, Doco, Thierry, Coulon, Joana, Massot, Arnaud, Vallet-Courbin, Amélie, Maupeu, Julie, Unité de Recherche Oenologie [Villenave d'Ornon], Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV), Institut Pascal - Clermont Auvergne (IP), Sigma CLERMONT (Sigma CLERMONT)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Bio-Laffort, Microflora, Institut des Sciences de la Vigne et du Vin, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Unité de Recherche Oenologie [Villenave d'Ornon] (OENO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), and Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Vegetal Biology, vin, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, chitosan, wine, chitosane, Biologie végétale, Sciences agricoles, Agricultural sciences
Abstract

Research on the origin and the side effects of chitosan stabilizing properties in wine. Applied Natural Products : From Discoveries To Innovations

Published
2018

27. Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains.

Author

Deamici, Kricelle M., de Morais, Michele G., Santos, Lucielen O., Muylaert, Koenraad, Gardarin, Christine, Costa, Jorge Alberto V., and Laroche, Céline

Subjects
MAGNETIC field effects, POLYSACCHARIDES, MICROBIAL exopolysaccharides, MICROALGAE, MONOSACCHARIDES, MICROBIAL metabolism, BIOMOLECULES, URONIC acids
Abstract

Microalgae are able to produce many valuable biomolecules, such as polysaccharides, that presents a large diversity of biochemical structures and functions as antioxidant, antifungal, anticancer, among others. Static magnetic fields (SMF) influence the metabolism of microorganisms and has been shown as an alternative to increase microalgae biomass, yield and compounds production. Especially, some studies have highlighted that SMF application could enhance carbohydrate content. This study aimed to evaluate different conditions of SMF on Spirulina and Chlorella in indoor and outdoor conditions, in order to confirm the influence of SMF on polysaccharides production, evaluating which polysaccharidic fraction could be enhanced by SMF and highlighting a possible modification in EPS composition. Starch from Chlorella and exopolysaccharides (EPS) from Spirulina were quantified and characterized. SMF increased the starch content in Chorella fusca biomass. EPS productions from A. platensis and Spirulina sp. were not significantly increased, and global composition appeared similar to the controls (constituted basically of 80–86% neutral sugars and 13–19% uronic acids). However, the monosaccharide composition analysis revealed a significant modification of composition, i.e., the amount of fucose, arabinose, rhamnose, galactose and glucuronic acid was increased, while the glucose content was decreased. SMF application led to significant modification of polysaccharides production and this study demonstrate that combining the outdoor conditions with SMF, the starch content and EPS composition was positively affected. [ABSTRACT FROM AUTHOR]

Published
2021
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28. Beneficial Health Potential of Algerian Polysaccharides Extracted from Plantago ciliata Desf. (Septentrional Sahara) Leaves and Seeds.

Author

Addoun, Noura, Boual, Zakaria, Delattre, Cédric, Chouana, Toufik, Gardarin, Christine, Dubessay, Pascal, Benaoun, Fatima, Addaoud, Seddik, Ould El Hadj, Mohamed Didi, Michaud, Philippe, Pierre, Guillaume, and Seca, Ana M. L.

Subjects
PLANTAGO, CILIATA, POLYSACCHARIDES, INDIGENOUS plants, FOURIER transform infrared spectroscopy, PECTINS, HEALERS
Abstract

Featured Application: This study substantially contributes to raising the understanding, safety, and efficiency of ethnobotanical remedies from Plantago ciliata employed by traditional healers in various Algerian health disorders. Today, an ethnobotanical approach makes sense for identifying new active bioactive chemicals from uses of indigenous plants. Two water-soluble enriched polysaccharide fractions (L-PSPN and S-PSPN) were extracted by hot water extraction from the leaves and seeds of Plantago ciliata Desf. (1798), a Mzab indigenous herb currently used in Algeria by traditional healers. Primary investigation was performed for describing the main structural features of these polysaccharides (pectin- and heteroxylan-like compositions) by using colorimetric assays, FTIR spectroscopy, HPAEC/PAD and GC/MS-EI analyses. Some biological activities were also monitored, such as anticomplement, anti-inflammatory (phagocytic ability, NOX2 and MPO inhibitions) and anti-diabetic (α-amylase and α-glucosidase inhibitions). L-PSPC seems able to moderately modulate innate immune system (IC50 around 100 µg/mL) and contribute to wound-healing processes (IC50 close to 217 vs. 443 µg/mL for sodium heparin). S-PSPC shows some potential as an anti-hyperglycemic (IC50 around 4.7 mg/mL) and anti-inflammatory (IC50 ranging from 111 to 203 µg/mL) agent, as well as other (fiber) psyllium-like polysaccharides extracted from Plantago species. [ABSTRACT FROM AUTHOR]

Published
2021
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29. Oligosaccharides biologiquement actifs obtenus par dégradation enzymatique d’un arabinogalactane extrait de cladodes de Cereus triangularis

Author

Petera, Benjamin, Delattre, Cédric, Pierre, Guillaume, Gardarin, Christine, Fenoradosoa, Taratra-Andrée, Michaud, Philippe, DUGAT, Pascale, Institut Pascal (IP), and SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

30. Researh on the origin and the side effects of chitosan stabilizing properties in wine

Author

Dols-Lafargue, M., Paulin, M., Miot Sertier, C., Ballestra, P., Albertin, W., Marchal, A., Masneuf Pomarede, I., Delattre, Cédric, Pierre, Guillaume, Dubessay, Pascal, Gardarin, Christine, Michaud, Philippe, Doco, T., Coulon, J., MASSOT, A., Vallet-Courbin, A., Maupeu, J., inconnu, Inconnu, Centre National d'Études Spatiales [Toulouse] (CNES), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut E3M [CHU Pitié-Salpêtrière], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])

Subjects
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

31. Caractérisation structurale et étude des propriétés physico-chimiques et biologiques de deux polysaccharides extraits des plantes africaines, Plantago notata et Astragalus armatus

Author

Pierre, Guillaume, Delattre, Cédric, Benaoun, Fatima, Boual, Zakaria, Gardarin, Christine, Vial, Christophe, Wadouachi, Anne, Le Cerf, Didier, Ould El-Hadj, Mohamed Didi, Michaud, Philippe, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Protection des Ecosystèmes en Zones arides et semi-arides, Université Kasdi Merbah Ouargla, Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Pierre, Guillaume, SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

32. De l’histoire de l’ethnobotanisme en Afrique vers la découverte de nouvelles sources de polysaccharides actifs

Author

Delattre, Cédric, Pierre, Guillaume, Petera, Benjamin, Chouana Toufik, Boual, Zakaria, Gardarin, Christine, Vial, Christophe, Wadouachi, Anne, Cerf, Didier Le, El-Hadj, Mohamed Didi Ould, Michaud, Philippe, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Faculté des Sciences, Université d'Antsiranana, B.P. 0, Antsiranana 201, Université d'Antsiranana (UNA), Laboratoire de Protection des Ecosystèmes en Zones arides et semi-arides, Université Kasdi Merbah Ouargla, Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Pierre, Guillaume, and Institut de Chimie du CNRS (INC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

33. Caractérisations structurale, physico-chimique et biologique des polysaccharides extraits d’une algue brune tunisienne, Cystoseira compressa

Author

Hentati, Faiez, Pierre, Guillaume, Delattre, Cédric, Desbrieres, Jacques, Le Cerf, Didier, Gardarin, Christine, Attia, H., Abdelkafi, Slim, Michaud, Philippe, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), École Nationale d'Ingénieurs de Sfax | National School of Engineers of Sfax (ENIS), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

34. Bioactive polysaccharides from African terrestrial plants: the importance of ethnobotany

Author

Pierre, Guillaume, Delattre, Cédric, Benaoun, Fatima, Chouana, Toufik, Petera, Benjamin, Gardarin, Christine, Vial, Christophe, Wadouachi, Anne, Le Cerf, Didier, Boual, Zakaria, Fenoradosoa, Andrée, Ould El-Hadj, Mohamed Didi, Michaud, Philippe, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire de Protection des Ecosystèmes en Zones arides et semi-arides, Université Kasdi Merbah Ouargla, Faculté des Sciences de l’Université d’Antsiranana, Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Pierre, Guillaume, SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut de Chimie du CNRS (INC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

35. Structural characterization and physico-chemical potential of a heteroxylan extracted from Plantago notata Lagasca (Plantaginaceae) seeds

Author

Benaoun, Fatima, Pierre, Guillaume, Delattre, Cédric, Chouana, Toufik, Petera, Benjamin, Gardarin, Christine, Vial, Christophe, Wadouachi, Anne, Le Cerf, Didier, Boual, Zakaria, Fenoradosoa, Taratra Andrée, Ould El-Hadj, Mohamed Didi, Dussap, C-Gilles, Michaud, Philippe, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire de Protection des Ecosystèmes en Zones arides et semi-arides, Université Kasdi Merbah Ouargla, Faculté des Sciences de l’Université d’Antsiranana, Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Pierre, Guillaume

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

36. Prebiotic Activity of Poly- and Oligosaccharides Obtained from Plantago major L. Leaves.

Author

Lukova, Paolina, Nikolova, Mariana, Petit, Emmanuel, Elboutachfaiti, Redouan, Vasileva, Tonka, Katsarov, Plamen, Manev, Hristo, Gardarin, Christine, Pierre, Guillaume, Michaud, Philippe, Iliev, Ilia, and Delattre, Cédric

Subjects
MONOSACCHARIDES, OLIGOSACCHARIDES, GEL permeation chromatography, PLANTAGO, GALACTURONIC acid, FRUCTOOLIGOSACCHARIDES, PHOTODETECTORS
Abstract

The aim of the present study was to evaluate the prebiotic potential of Plantago major L. leaves water-extractable polysaccharide (PWPs) and its lower molecular fractions. The structure of PWPs was investigated by high pressure anion exchange chromatography (HPAEC), size exclusion chromatography coupled with multi-angle laser light scattering detector (SEC-MALLS) and Fourier-transform infrared (FTIR) spectroscopy. The chemical composition and monosaccharide analyses showed that galacturonic acid was the main monosaccharide of PWPs followed by glucose, arabinose, galactose, rhamnose and xylose. FTIR study indicated a strong characteristic absorption peak at 1550 cm−1 corresponding to the vibration of COO− group of galacturonic acid. The PWPs was subjected to hydrolysis using commercial enzymes to obtain P. major low molecular fraction (PLM) which was successively separated by size exclusion chromatography on Biogel P2. PWPs and PLM were examined for in vitro prebiotic activity using various assays. Results gave evidence for changes in optical density of the bacteria cells and pH of the growth medium. A heterofermentative process with a lactate/acetate ratio ranged from 1:1 to 1:5 was observed. The ability of PLM to stimulate the production of certain probiotic bacteria glycohydrolases and to be fermented by Lactobacillus sp. strains was successfully proved. [ABSTRACT FROM AUTHOR]

Published
2020
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37. Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens.

Author

Hentati, Faiez, Delattre, Cédric, Gardarin, Christine, Desbrières, Jacques, Le Cerf, Didier, Rihouey, Christophe, Michaud, Philippe, Abdelkafi, Slim, and Pierre, Guillaume

Subjects
RED algae, NUCLEAR magnetic resonance spectroscopy, GEL permeation chromatography, INTRINSIC viscosity, INFRARED spectroscopy, CERAMIALES
Abstract

A novel sulfated xylogalactan-rich fraction (JSP for J. adhaerens Sulfated Polysaccharide) was extracted from the red Tunisian seaweed Jania adhaerens. JSP was purified using an alcoholic precipitation process and characterized by Attenuated Total Reflectance-Fourier-transform infrared spectroscopy (ATR-FTIR), high-pressure size exclusion chromatography (HPSEC) with a multi-angle laser light scattering (MALLS), gas chromatography coupled to mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR, 1D and 2D). JSP was then evaluated regarding its physicochemical and rheological properties. Results showed that JSP was mainly composed of an agar-like xylogalactan sharing the general characteristics of corallinans. The structure of JSP was mainly composed of agaran disaccharidic repeating units (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→)n and (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→)n, mainly substituted on O-6 of (1,3)-β-d-Galp residues by β-xylosyl side chains, and less with sulfate or methoxy groups. (1,4)-α-l-Galp residues were also substituted by methoxy and/or sulfate groups in the O-2 and O-3 positions. Mass-average and number-average molecular masses (Mw) and (Mn), intrinsic viscosity ([η]) and hydrodynamic radius (Rh) for JSP were, respectively, 8.0 × 105 g/mol, 1.0 × 105 g/mol, 76 mL/g and 16.8 nm, showing a flexible random coil conformation in solution. The critical overlap concentration C* of JSP was evaluated at 7.5 g/L using the Williamson model. In the semi-diluted regime, JSP solutions displayed a shear-thinning behavior with a great viscoelasticity character influenced by temperature and monovalent salts. The flow characteristics of JSP were described by the Ostwald model. [ABSTRACT FROM AUTHOR]

Published
2020
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39. Epoxy CIM® Disk comme nouveaux supports d’immobilisation de dextranases pour la production continue d’isomaltooligosaccharides

Author

Pierre, Guillaume, Bertrand, Emmanuel, Delattre, Cédric, Gardarin, Christine, Chalane, Seltanna, Bridiau, Nicolas, Maugard, Thierry, Štrancar, Aleš, Michaud, Philippe, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), BIA Separations d.o.o, LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), and Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

41. EXTRACTION OF BIOMOLECULES FROM A BROWN SEAWEED FROM THE ROMANIAN BLACK SEA SHORE (CYSTOSEIRA BARBATA).

Author

TRICĂ, Bogdan, DELATTRE, Cédric, PIERRE, Guillaume, URSU, Alina-Violeta, GARDARIN, Christine, MICHAUD, Philippe, DJELVEH, Gholamreza, and DOBRE, Tănase

Subjects
CYSTOSEIRA, MARINE algae, SPECIES distribution, PHENOLS, BIOMOLECULES
Abstract

Cystoseira barbata, the main brown seaweed species at the Romanian Black Sea shore, contains a number of valuable biomolecules. We focus on the extraction of alginate, fucans and phlorotannins. Alginate is already a natural biomolecule with a well-established market,which is supposed to grow in the near future, as a thickening and gelling agent. Furthermore, fucans have recently been shown to be biologically active. Phlorotannins are also worth investigating as they are polymers of phloroglucinol, a phenolic compound which has a good antioxidant activity. In this paper, alginate, fucans and phlorotannins are extracted in a sequential manner from Cystoseira barbata. Identification and quantification of the biomolecules is carried out by specific methods. The purpose of this paper is to prove the concept of biorefinery applied for a brown seaweed species at the Romanian Black Sea shore. [ABSTRACT FROM AUTHOR]

Published
2017

42. Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer.

Author

Gargouch, Nesrine, Elleuch, Fatma, Karkouch, Ines, Tabbene, Olfa, Pichon, Chantal, Gardarin, Christine, Rihouey, Christophe, Picton, Luc, Abdelkafi, Slim, Fendri, Imen, Laroche, Céline, Yermak, Irina M., and Davydova, Viktoria

Abstract

Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers. [ABSTRACT FROM AUTHOR]

Published
2021
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43. Optimization of Exopolysaccharides Production by Porphyridium sordidum and Their Potential to Induce Defense Responses in Arabidopsis thaliana against Fusarium oxysporum.

Author

Drira, Marwa, Elleuch, Jihen, Ben Hlima, Hajer, Hentati, Faiez, Gardarin, Christine, Rihouey, Christophe, Le Cerf, Didier, Michaud, Philippe, Abdelkafi, Slim, and Fendri, Imen

Subjects
FUSARIUM oxysporum, ARABIDOPSIS thaliana, DEFENSINS, CATALASE, SALICYLIC acid, CYTOCHROME P-450, FUNGAL growth, PLANT defenses
Abstract

Polysaccharides from marine algae are one novel source of plant defense elicitors for alternative and eco-friendly plant protection against phytopathogens. The effect of exopolysaccharides (EPS) produced by Porphyridium sordidum on elicitation of Arabidopsis thaliana defense responses against Fusarium oxysporum was evaluated. Firstly, in order to enhance EPS production, a Box–Behnken experimental design was carried out to optimize NaCl, NaNO3 and MgSO4 concentrations in the culture medium of microalgae. A maximum EPS production (2.45 g/L) higher than that of the control (0.7 g/L) was observed for 41.62 g/L NaCl, 0.63 g/L NaNO3 and 7.2 g/L MgSO4 concentrations. Structurally, the EPS contained mainly galactose, xylose and glucose. Secondly, the elicitor effect of EPS was evaluated by investigating the plant defense-related signaling pathways that include activation of Salicylic or Jasmonic Acid-dependent pathway genes. A solution of 2 mg/mL of EPS has led to the control of fungal growth by the plant. Results showed that EPS foliar application induced phenylalaline ammonia lyase and H2O2 accumulation. Expression profile analysis of the defense-related genes using qRT-PCR revealed the up-regulation of Superoxide dismutases (SOD), Peroxidase (POD), Pathogenesis-related protein 1 (PR-1) and Cytochrome P450 monooxyge-nase (CYP), while Catalase (CAT) and Plant defensin 1.2 (PDF1.2) were not induced. Results suggest that EPS may induce the elicitation of A. thaliana's defense response against F. oxysporum, activating the Salicylic Acid pathway. [ABSTRACT FROM AUTHOR]

Published
2021
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44. Induction of Natural Defenses in Tomato Seedlings by Using Alginate and Oligoalginates Derivatives Extracted from Moroccan Brown Algae.

Author

Aitouguinane, Meriem, Bouissil, Soukaina, Mouhoub, Anouar, Rchid, Halima, Fendri, Imen, Abdelkafi, Slim, Ould El-Hadj, Mohamed Didi, Boual, Zakaria, Dubessay, Pascal, Gardarin, Christine, Michaud, Philippe, El Alaoui-Talibi, Zainab, El Modafar, Cherkaoui, Pierre, Guillaume, and Delattre, Cédric

Abstract

Polysaccharides extracted from marine algae have attracted much attention due to their biotechnological applications, including therapeutics, cosmetics, and mainly in agriculture and horticulture as biostimulants, biofertilizers, and stimulators of the natural defenses of plants. This study aimed to evaluate the ability of alginate isolated from Bifurcaria bifurcata from the Moroccan coast and oligoalginates derivatives to stimulate the natural defenses of tomato seedlings. Elicitation was carried out by the internodal injection of bioelicitor solutions. The elicitor capacities were evaluated by monitoring the activity of phenylalanine ammonia-lyase (PAL) as well as polyphenols content in the leaves located above the elicitation site for 5 days. Alginate and oligoalginates treatments triggered plant defense responses, which showed their capacity to significantly induce the PAL activity and phenolic compounds accumulation in the leaves of tomato seedlings. Elicitation by alginates and oligoalginates showed an intensive induction of PAL activity, increasing from 12 h of treatment and remaining at high levels throughout the period of treatment. The amount of polyphenols in the leaves was increased rapidly and strongly from 12 h of elicitation by both saccharide solutions, representing peaks value after 24 h of application. Oligoalginates exhibited an effective elicitor capacity in polyphenols accumulation compared to alginate polymers. The alginate and oligosaccharides derivatives revealed a similar elicitor capacity in PAL activity whereas the accumulation of phenolic compounds showed a differential effect. Polysaccharides extracted from the brown seaweed Bifurcaria bifurcate and oligosaccharides derivatives induced significantly the phenylpropanoid metabolism in tomato seedlings. These results contribute to the valorization of marine biomass as a potential bioresource for plant protection against phytopathogens in the context of eco-sustainable green technology. [ABSTRACT FROM AUTHOR]

Published
2020
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46. Characterization of a new exopolysaccharide produced by Halorubrum sp. TBZ112 and evaluation of its anti-proliferative effect on gastric cancer cells.

Author

Hamidi, Masoud, Mirzaei, Rasool, Delattre, Cédric, Khanaki, Korosh, Pierre, Guillaume, Gardarin, Christine, Petit, Emmanuel, Karimitabar, Fatemeh, and Faezi, Sobhan

Subjects
STOMACH cancer, MICROBIAL exopolysaccharides, CANCER cells, BIOCOMPATIBILITY, FIBROBLASTS
Abstract

In the present study, we aimed to extract, purify, analyze monosaccharide composition of  exopolysaccharide (EPS) produced by Halorubrum sp. TBZ112 (KCTC 4203 and IBRC-M 10773) and also to evaluate its possible antiproliferative activity against human gastric cancer (MKN-45) cell line and its biocompatibility effect on normal cells using human dermal fibroblast (HDF) cell line. Average molecular weight and monosaccharide composition were determined by high-pressure size exclusion chromatography (HPSEC) with multi-angle laser light scattering (MALLS) and high-pressure anion exchange chromatography (HPAEC), respectively. Fourier transform infrared (FTIR) spectroscopy was used for the partial characterization of the EPS. The EPS effect on the cell proliferation and viability of MKN-45 and HDF cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and trypan blue dye exclusion, respectively. Strain TBZ112 excreted 480 mg.l−1 of the EPS under optimal growth conditions. The EPS had a molecular weight of 5.052 kDa and was a heteropolysaccharide containing ten moieties mainly composed of mannose (19.95%), glucosamine (15.55%), galacturonic acid (15.43%), arabinose (12.24%), and glucuronic acid (12.05%). No significant difference of the EPS treatments on the proliferation activity of MKN-45 and HDF cells were observed (P > 0.05). For the first time, the EPS from Halorubrum sp. TBZ112, an extremely halophilic archaeon related to Halorubrum genus, was isolated and chemically characterized. The EPS from Halorubrum sp. TBZ112 possesses a relatively low molecular weight and might be applied as a biocompatible compound. More investigations are needed to determine other biological activities of the EPS along with further details of its chemical structure. [ABSTRACT FROM AUTHOR]

Published
2018
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