14 results on '"Folliculogenèse"'
Search Results
2. The mode of ovulation in the Namaqua rock mouse, Micaelamys namaquensis.
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Relton, C.E., Bennett, N.C., and Medger, K.
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MICE reproduction , *OVULATION , *ANIMAL sexual behavior , *OVARIAN follicle , *CORPUS luteum , *ZOOLOGY , *MAMMALS - Abstract
The pattern of ovulation in mammals is generally considered to be either spontaneous or induced by copulation, with environmental and social factors playing a key role in determining the specific mode of ovulation that would maximize the reproductive potential of the species. This study aims to determine whether the Namaqua rock mouse ( Micaelamys namaquensis (A. Smith, 1834)) is a spontaneous or induced ovulator. Females were divided into three treatments differing in the degree of contact with a male. Namely, seven control females had no contact with a male; a further seven separated females had only chemical, auditory, and visual contact with a male; whereas six females had intermittent periods of full contact with a vasectomized male. Ovarian size, follicular development, and plasma progesterone concentrations were compared between the three treatments. Penile morphology was also investigated. Corpora lutea were found in all three treatments and the penis was smooth without any ridges or spikes, indicating that M. namaquensis is a spontaneous ovulator. Interestingly, however, the presence of a male (physical as well as just visual and olfactory) positively affected ovarian size, ovulation, and plasma progesterone concentrations. This signifies that although M. namaquensis ovulates spontaneously, male contact significantly influences ovulation rate and ultimately reproductive success. [ABSTRACT FROM AUTHOR]
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- 2013
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3. Bidirectional communication between oocytes and follicle cells: ensuring oocyte developmental competence.
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Kidder, Gerald M. and Vanderhyden, Barbara C.
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OVUM , *MEIOSIS , *OOGENESIS , *FEMALE infertility , *CELL receptors , *GAP junctions (Cell biology) ,RISK factors in infertility - Abstract
Female fertility is determined to a large extent by the quality (developmental competence) of the oocyte as reflected in its ability to undergo meiosis, be fertilized, and give rise to a healthy embryo. Growth of the mammalian oocyte is coordinated with that of the follicle that encloses it by the actions of signals that pass in both directions between the germline and somatic components. This review summarizes what is known about the roles played by 2 different modes of intrafollicular signalling in oogenesis: paracrine factors activating receptors on the opposite cell type, and direct sharing of small molecules throughout the follicle via gap junction channels. Recent evidence indicates that these 2 modes of signalling interact to regulate oocyte growth and granulosa cell proliferation and that defects in either can contribute to female infertility. La fertilité féminine est déterminée en grande partie par la qualité (compétence au développement) de l’ovocyte, caractérisée par sa capacité d’entrer en méiose, d’être fertilisé et de former un embryon sain. Chez les mammifères, la croissance de l’ovocyte est coordonnée avec celle du follicule qui l’englobe sous l’action des signaux échangés entre les cellules germinales et les composantes somatiques. La présente synthèse résume les connaissances sur les rôles joués par deux modes de signalisation intrafolliculaire durant l’ovogenèse: les facteurs paracrines activant les récepteurs sur le type cellulaire opposé, et le partage direct de petites molécules dans le follicule par l’intermédiaire des jonctions communicantes. Des résultats récents indiquent que ces deux modes de signalisation interagissent pour réguler la croissance des ovocytes et la prolifération des cellules de la granulosa, et que des anomalies de l’un ou l’autre pourraient contribuer à l’infertilité féminine. [ABSTRACT FROM AUTHOR]
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- 2010
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4. Vitellogenesis and yolk proteins, Birds
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Nicolas Guyot, Sophie Réhault-Godbert, Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Penny Swanson, Michael K. Skinner, and Recherches Avicoles (SRA)
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animal structures ,food.ingredient ,media_common.quotation_subject ,[SDV]Life Sciences [q-bio] ,Zoology ,embryo ,vitellogénèse ,Biology ,01 natural sciences ,Internal fertilization ,03 medical and health sciences ,Telolecithal ,food ,folliculogenesis ,Yolk ,Biochemical composition ,oiseau ,030304 developmental biology ,media_common ,Yolk Proteins ,0303 health sciences ,folliculogénèse ,010401 analytical chemistry ,Embryo ,poule ,0104 chemical sciences ,embryon ,embryonic structures ,oeuf ,egg ,Vitellogenesis ,Reproduction - Abstract
Reproduction in Birds is characterized by internal fertilization and external development of an embryo in a telolecithal egg containing all nutrients, biological activities and protective systems required for the proper growth of the embryo, until hatch. This article will give an overview on the chicken ( Gallus gallus ) egg yolk proteins and vitellogenesis, knowing that the formation and structure of the egg (including the yolk) is quite conserved, regardless of the Bird species, although some differences exist in the timing and number of clutches of eggs produced, and in some specific egg traits (egg weight, yolk/white proportion, biochemical composition, etc.).
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- 2017
5. [i]Dmxl2[/i] is involved in follicle formation in the mouse
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El Zaiat, Maëva, Gobe, Clara, Passet, Bruno, Meunier, Nicolas, André, Marjolaine, Jouneau, Luc, Congar, Patrice, Bonnet, Aurelie, Pailhoux, Eric, Pannetier, Maëlle, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Neurobiologie de l'olfaction (NBO), Institut National de la Recherche Agronomique (INRA), ANR, FRM, Université de Haute Bretagne ( Rennes 2 ) (UR 2). Rennes, FRA., Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech
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fertilité ,endocrine system ,folliculogénèse ,allogreffes ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,létalité néonatale ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,dmxl2 - Abstract
We identified DMXL2 from RNA-sequencing data as a gene preferentially expressed in differentiating-ovaries in the goat species. In order to determine its role during ovarian morphogenesis, we investigated functional studies in mice. Dmxl2 invalidation (KO) is lethal within the few hours following birth. The analysis of its expression in several tissues let us show that the heart, olfactory mucosa and brain expressed Dmxl2 during development. Our results further suggest that the lethality may be due to an alteration of the synaptic transmission.Besides, both male and female gonads express Dmxl2. Notably in the ovary, its expression increases few days before follicle formation. We show that DMXL2 protein is dynamically expressed in germ cells and granulosa cells; DMXL2 is strongly detected in the oocytes whereas its expression in the granulosa cells fluctuates depending on the stage of folliculogenesis. To study Dmxl2 implication during follicle formation, and to bypass the issue of lethality at birth, we performed allograft of KO ovaries on nude recipient mice. KO grafts show an alteration in follicle formation, followed by ovarian dysgenesis.To conclude, our work shows that Dmxl2 is involved in follicle formation in mice, and makes it a new candidate to explain some cases of premature ovarian failure. The conditional knock-out of Dmxl2 in germ cells or in granulosa cells is in progress in the laboratory (please refer to Clara Gobé’s poster) and will permit to precise its specific role in each cell types.
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- 2015
6. Specific deletion of AMP-activated protein kinase (α1AMPK) in murine oocytes alters junctional protein expression and mitochondrial physiology
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Marc Foretz, Benoit Viollet, Melanie Faure, Joëlle Dupont, Pascal Froment, Michael J. Bertoldo, Christelle Ramé, Edith Guibert, ProdInra, Archive Ouverte, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), School of Women's and Children's Health, University of New South Wales [Sydney] (UNSW), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Melanie Faure was supported by theRegion Centre and Institut National de la RechercheAgronomique (Ph.D Grant). The nationalFERTiNERGY programme funded by the FrenchNational Research Agency (ANR), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Discipline of Obstetrics and Gynaecology, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] ( PRC ), Institut National de la Recherche Agronomique ( INRA ) -Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique ( CNRS ), University of New South Wales [Sydney] ( UNSW ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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Male ,Cell signaling ,[SDV]Life Sciences [q-bio] ,amp protéine kinase ,lcsh:Medicine ,Mice, Transgenic ,Cell Communication ,Biology ,souris ,AMP-Activated Protein Kinases ,immunohistochimie ,Oogenesis ,Connexins ,Paracrine signalling ,Mice ,femelle ,Ovarian Follicle ,medicine ,Animals ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Phosphorylation ,Histone H3 acetylation ,Protein kinase A ,lcsh:Science ,[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Multidisciplinary ,folliculogénèse ,fécondation in vitro ,Granulosa Cells ,ovogénèse ,lcsh:R ,Gap Junctions ,Oocyte ,Cadherins ,Cell biology ,Mitochondria ,Mice, Inbred C57BL ,[SDV] Life Sciences [q-bio] ,medicine.anatomical_structure ,Oocytes ,lcsh:Q ,Female ,Histone deacetylase activity ,Folliculogenesis ,Energy Metabolism ,Research Article ,Signal Transduction - Abstract
Oogenesis and folliculogenesis are dynamic processes that are regulated by endocrine, paracrine and autocrine signals. These signals are exchanged between the oocyte and the somatic cells of the follicle. Here we analyzed the role of AMP-activated protein kinase (AMPK), an important regulator of cellular energy homeostasis, by using transgenic mice deficient in α1AMPK specifically in the oocyte. We found a decrease of 27% in litter size was observed in ZP3-α1AMPK-/- (ZP3-KO) female mice. Following in vitro fertilization, where conditions are stressful for the oocyte and embryo, ZP3-KO oocytes were 68% less likely to pass the 2-cell stage. In vivo and in cumulus-oocyte complexes, several proteins involved in junctional communication, such as connexin37 and N-cadherin were down-regulated in the absence of α1AMPK. While the two signalling pathways (PKA and MAPK) involved in the junctional communication between the cumulus/granulosa cells and the oocyte were stimulated in control oocytes, ZP3-KO oocytes exhibited only low phosphorylation of MAPK or CREB proteins. In addition, MII oocytes deficient in α1AMPK had a 3-fold lower ATP concentration, an increase in abnormal mitochondria, and a decrease in cytochrome C and PGC1α levels, suggesting perturbed energy production by mitochondria. The absence of α1AMPK also induced a reduction in histone deacetylase activity, which was associated with an increase in histone H3 acetylation (K9/K14 residues). Together, the results of the present study suggest that absence of AMPK, modifies oocyte quality through energy processes and oocyte/somatic cell communication. The limited effect observed in vivo could be partly due to a favourable follicle microenvironment where nutrients, growth factors, and adequate cell interaction were present. Whereas in a challenging environment such as that of in vitro culture following IVF, the phenotype is revealed.
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- 2015
7. Molecular and mechanical regulators of lymphatic biology
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Rutkowski, Joseph Michael and Swartz, Melody
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lymphoedème ,lymphatiques ,folliculogénèse ,lymphangiogénèse ,extracellular matrix ,flux interstitiel ,matrice extracellulaire ,VEGF-C ,lymphedema ,interstitial flow ,VEGFR-3 ,lymphangiogenesis ,lymphatic ,folliculogenesis - Abstract
Lymphatic vessels exist in nearly all tissues, yet, despite their omnipresence, there remains a large knowledge gap between the described fundamental roles of lymphatic capillaries and our understanding of their functional biology, adaptive ability, and pathological response. This thesis addressed these shortcomings by utilizing an integrative biomedical engineering approach to examine molecular and mechanical regulators of lymphatic capillaries using in vivo models of lymphatic capillary biology, function, and adaptation. Using a model of skin regeneration in the mouse tail, we demonstrated that slow interstitial flow created by lymphatic drainage was necessary for lymphatic capillary organization. This novel model permitted the identification of spatial, temporal and chemical factors governing lymphangiogenesis. In contrast to the sprouting mechanism of blood angiogenesis, lymphatic endothelial cells (LECs) were demonstrated to organize in a vasculogenesis-like manner, migrating in the direction of interstitial flow and then organizing into functional lymphatic capillaries. Lymphangiogenesis was inhibited by blocking vascular endothelial growth factor (VEGF)-C signaling from day 0, but initiation of receptor blockade once LECs had already migrated did not prevent vessel organization. This uniquely demonstrated the need for a biochemical mediator (VEGF-C) to initiate lymphangiogenesis, but that an important biomechanical force, interstitial flow, was necessary for functional capillary organization. Further insight into the necessity of interstitial flow in LEC biology was found in the response of lymphatic capillary to induced lymphedema, wherein lymphatic drainage is significantly reduced. In a mouse tail model of secondary lymphedema, we demonstrated that the edematous environment – characterized by extracellular matrix breakdown, lipid accumulation, and reduced interstitial flow – resulted in hyperplasia of LECs but concurrent poor function due to the lack of interstitial flow as an organizational guiding cue. Similar dermal matrix adaptations to dysfunctional lymphatic drainage were also noted in two mouse models of congenital lymphedema, the Chy and VEGFR-3-Ig mice, further demonstrating the intimate connection of lymphatic capillary function with tissue maintenance and remodeling. To quantitatively demonstrate the changes in lymphatic capillary uptake and tissue hydraulic conductivity found in these and other transgenic mouse models, we developed a poroelastic model of interstitial transport. Tissue hydraulic conductivity was also calculated in tissues lacking lymphatics using an unsteady-state solution, demonstrating that lymphedema causes a significant increase in tissue conductivity. This model was then utilized to assess the effects of a high fat diet, metabolic disorders, and lymphatic dysfunction on the tissue and on lymphatic capillary function. We discovered that lymphatic capillary uptake function was significantly reduced with dyslipidemia, suggesting a novel interplay between lymphatic function and lipid metabolism. Additionally, we uncovered a new and critical role for lymphangiogenesis and lymphatic transport in reproduction. We demonstrated that lymphangiogenesis is a regular, non-pathological event during folliculogenesis in the ovary. These new lymphatic capillaries are seemingly necessary for hormone transport from the ovary – an essential feedback mechanism during pregnancy. Blockade of lymphangiogenesis resulted in decreased systemic progesterone and estradiol levels and resulted in failed fetal development. In conclusion, this work highlights the critical roles of the lymphatic circulation and demonstrates the interplay between lymphatic biology and the biochemical and biophysical environment in which lymphatic capillaries reside. Interstitial flow and the interstitium modulate lymphatic behavior, and lymphatic function, in turn, controls the tissue microenvironment.
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- 2008
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8. Intrafollicular steroids and anti-Müllerian hormone during normal and cystic ovarian follicular development in the cow
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di Clemente, Nathalie, Touze, Jean-Luc, Belville, Corinne, Rico, Charlène, Bontoux, Martine, Picard, Jean-Yves, Fabre, Stéphane, and Monniaux, Danielle
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endocrine system ,Reproductive Biology ,bovin ,folliculogénèse ,ovaire ,reproduction animale ,hormone antimullérienne ,steroidogénèse ,parasitic diseases ,anti mullerian hormone ,follicle ,follicular development ,ovary ,steroid hormone ,vache ,Biologie de la reproduction ,steroïde ,kyste ovarien ,développement folliculaire ,follicule - Abstract
Development of follicular cysts is a frequent ovarian dysfunction in cattle. Functional changes that precede cyst formation are unknown, but a role for anti-Mullerian hormone (AMH) in the development of follicular cysts has been suggested in humans. This study aimed to characterize intrafollicular steroids and AMH during follicular growth in a strain of beef cows exhibiting a high incidence of occurrence of follicular cysts. Normal follicular growth and cyst development were assessed by ovarian ultrasonography scanning during the 8 days before slaughtering. Experimental regression of cysts was followed by rapid growth of follicles that reached the size of cysts within 3-5 days. These young cysts exhibited higher intrafollicular concentrations of testosterone, estradiol-17beta, and progesterone than large early dominant follicles did in normal ovaries, but they exhibited similar concentrations of AMH. Later-stage cysts were characterized by hypertrophy of theca interna cells, high intrafollicular progesterone concentration, and high steroidogenic acute regulatory protein mRNA expression in granulosa cells. Progesterone and AMH concentrations in the largest follicles (>= 10 mm) and cysts were negatively correlated (r = -0.45, P < 0.01). Smaller follicles (< 10 mm) exhibited higher intrafollicular testosterone and estradiol-17beta concentrations in ovaries with cysts compared to normal ovaries. During follicular growth, AMH concentration dropped in follicles larger than 5 mm in diameter and in a similar way in ovaries with and without cysts. In conclusion, enhanced growth and steroidogenesis in antral follicles < 10 mm preceded cyst formation in cow ovaries. Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization.
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- 2008
9. Identification of transcripts involved in meiosis and follicle formation during ovine ovary development
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Adrienne Baillet, Eric Pailhoux, Corinne Cotinot, Elodie Poumerol, Beatrice Mandon-Pepin, Cédric Cabau, INRA - Mathématiques et Informatique Appliquées (Unité MIAJ), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Unité de Recherches Avicoles (URA), and Centre National de la Recherche Scientifique (CNRS)
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Male ,[SDV]Life Sciences [q-bio] ,reproduction animale ,FOLLICULE OVARIEN ,Oogenesis ,0302 clinical medicine ,ovin ,Ovarian Follicle ,TRANSCRIPTS ,MEIOSIS ,FOLLICLE ,OVINE ,DEVELOPMENT ,ComputingMilieux_MISCELLANEOUS ,Genetics ,Expressed Sequence Tags ,0303 health sciences ,Expressed sequence tag ,folliculogénèse ,030219 obstetrics & reproductive medicine ,medicine.anatomical_structure ,FOLLICULOGENESIS ,Female ,Folliculogenesis ,Germ cell ,Biotechnology ,Research Article ,méiose ,lcsh:QH426-470 ,expression génique ,lcsh:Biotechnology ,Granulosa cell ,Biology ,03 medical and health sciences ,lcsh:TP248.13-248.65 ,Homologous chromosome ,medicine ,BIOLOGIE DU DEVELOPPEMENT ,Animals ,[INFO]Computer Science [cs] ,030304 developmental biology ,Sheep ,cDNA library ,Gene Expression Profiling ,ovaire ,Ovary ,Gene expression profiling ,lcsh:Genetics - Abstract
BackgroundThe key steps in germ cell survival during ovarian development are the entry into meiosis of oogonies and the formation of primordial follicles, which then determine the reproductive lifespan of the ovary. In sheep, these steps occur during fetal life, between 55 and 80 days of gestation, respectively. The aim of this study was to identify differentially expressed ovarian genes during prophase I meiosis and early folliculogenesis in sheep.ResultsIn order to elucidate the molecular events associated with early ovarian differentiation, we generated two ovary stage-specific subtracted cDNA libraries using SSH. Large-scale sequencing of these SSH libraries identified 6,080 ESTs representing 2,535 contigs. Clustering and assembly of these ESTs resulted in a total of 2,101 unique sequences depicted in 1,305 singleton (62.11%) and 796 contigs (37.9%) ESTs (clusters). BLASTX evaluation indicated that 99% of the ESTs were homologous to various known genes/proteins in a broad range of organisms, especially ovine, bovine and human species. The remaining 1% which exhibited any homology to known gene sequences was considered as novel. Detailed study of the expression patterns of some of these genes using RT-PCR revealed new promising candidates for ovary differentiation genes in sheep.ConclusionWe showed that the SSH approach was relevant to determining new mammalian genes which might be involved in oogenesis and early follicle development, and enabled the discovery of new potential oocyte and granulosa cell markers for future studies. These genes may have significant implications regarding our understanding of ovarian function in molecular terms, and for the development of innovative strategies to both promote and control fertility.
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- 2008
10. Opportunities and challenges in applying genomics to the study of oogenesis and follicullogenesis in farm animals
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Marc-André Sirard, R Dalbiès-Tran, Agnès Bonnet, Laboratoire de Génétique Cellulaire (LGC), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université Laval [Québec] (ULaval), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
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Embryology ,expression génique ,Population ,reproduction animale ,Genomics ,Context (language use) ,Computational biology ,Biology ,mammifère domestique ,Mice ,03 medical and health sciences ,Oogenesis ,0302 clinical medicine ,Endocrinology ,Ovarian Follicle ,RNA interference ,Biologie de la reproduction ,Animals ,education ,Oligonucleotide Array Sequence Analysis ,030304 developmental biology ,Reproductive Biology ,0303 health sciences ,education.field_of_study ,folliculogénèse ,030219 obstetrics & reproductive medicine ,ovogénèse ,Gene Expression Profiling ,ovaire ,Gene Expression Regulation, Developmental ,mammifère ,Obstetrics and Gynecology ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Cell Biology ,Genome project ,GENOMIQUE ,Gene expression profiling ,Reproductive Medicine ,développement embryonnaire ,Animals, Domestic ,Oocytes ,Cattle ,Female ,Folliculogenesis ,Functional genomics - Abstract
Ovarian oogenesis and folliculogenesis are complex and coordinated biological processes which require a series of events that induce morphological and functional changes within the follicle, leading to cell differentiation and oocyte development. In this context, the challenge of the researchers is to describe the dynamics of gene expression in the different compartments and their interactions during the follicular programme. In recent years, high-throughput arrays have become a powerful tool with which to compare the whole population of transcripts in a single experiment. Here, we review the challenges of applying genomics to this model in farm animal species. The first limitation lies in limited the availability of biological material, which makes the study of the follicle compartments (oocyte, granulosa cells and thecal cells) or early embryo much more difficult. The concept of observing all transcripts at once is very attractive but despite progress in sequencing, the genome annotation remains very incomplete in non-model species. Particularly, oogenesis and early embryo development relate to the high proportion of unknown expressed sequence tags. Then, it is important to consider post-transcriptional and translational regulation to understand the role of these genes. Ultimately, these new inferred insights will still have to be validated by functional approaches. In addition toin vitroorex vivofunctional approaches, both ‘natural mutant’ ewe models and RNA interference represent, at the moment, the best hope for functional genomics. Advances in our understanding of reproductive physiology should be facilitated by gene expression data exchange and translation into a better understanding of the underlying biological phenomena.
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- 2007
11. Follicular dynamics in Serrana goats
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Jorge Manuel Teixeira de Azevedo, Paulo J. Fontes, João Simões, Gérard Baril, José Carlos Almeida, Ramiro Mascarenhas, Ramiro Valentim, Universidade de Trás-os-Montes e Alto Douro, Instituto Politecnico de Braganca, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Instituto Nacional de Investigação Agrária (EVN), ProdInra, Migration, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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Male ,medicine.medical_specialty ,media_common.quotation_subject ,Ovary ,Estrous Cycle ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Animal science ,Follicular dynamics ,Food Animals ,Ovarian Follicle ,Internal medicine ,Luteolysis ,Follicular phase ,medicine ,Seasonal breeder ,Animals ,[SDV.SA.SPA] Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,Ovulation ,Progesterone ,ComputingMilieux_MISCELLANEOUS ,media_common ,Ultrasonography ,2. Zero hunger ,Estrous cycle ,CYCLE OESTRIEN ,030219 obstetrics & reproductive medicine ,business.industry ,Goats ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,Luteinizing Hormone ,040201 dairy & animal science ,Dominant follicle ,medicine.anatomical_structure ,[SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,Goat ,Animal Science and Zoology ,Female ,FOLLICULOGENESE ,business - Abstract
Twenty-two Serrana goats were studied through two successive estrous cycles in order to characterize their follicular dynamics during the breeding season. The ovaries of the goats were scanned daily by real-time ultrasonography and all folliclesor=3mm were measured and classified. The data were classified by the number of follicular waves per goat to test the hypothesis that temporal and morphological differences between the last follicular wave of an ovary, irrespective of ovulation, will affect the selection of the next ovulatory wave. The mean interovulatory interval was 20.7+/-1.0 days (mean+/-S.D.). Three to five waves per estrous cycle were observed and 61.3% (19/31) of cycles had four waves. In estrous cycles with four waves, the day of onset of the first, second, third and fourth wave was 1.4+/-1.0, 6.9+/-1.4, 11.6+/-1.8 and 16.8+/-1.6, respectively. No differences (P0.05) were found between the day of onset of the first and second waves for estrous cycles with three, four or five waves. However, the day of onset of the third and fourth waves occurred later when the number of waves per estrous cycle increased (P0.001). The duration of the interwave interval (time between the day of onset of two consecutive waves) was longer when the second wave was ovulatory. The length of the growth phase (2.4+/-0.9 days) and size (5.9+/-0.7 mm) of the dominant follicle in the second wave were lower (P0.01) than for the first wave (3.3+/-1.2 days and 6.6+/-0.9 mm, respectively) and the fifth wave (4.1+/-1.2 days and 7.5+/-1.0mm, respectively). Within pairs of ovaries, the onset of the last wave occurred later (P0.05) and was less variable in ovulatory ovaries (day 16.8+/-1.4, n=20) than in anovulatory ovaries (day 15.1+/-3.7, n=20). The length of the growing phase was longer (P0.001) in the last waves of ovulatory ovaries (3.1+/-0.9 days) than in the last waves of anovulatory ovaries (1.7+/-0.8 days). These results support the hypothesis that the day of onset of the ovulatory wave is related to or, at least, conditioned by the luteolysis and the decrease in plasma progesterone. In summary, the estrous cycle of Serrana goats is characterized by sequential follicular wave growth with a great variability in their onset and duration, with the exception of the ovulatory wave. The temporal and morphological differences observed in the last wave of estrous cycle provide strong evidence for the role of progesterone in their regulation.
- Published
- 2006
12. MATER protein expression and intracellular localization throughout folliculogenesis and preimplantion embryo development in the bovine
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Pennetier, Sophie, Perreau, Christine, Uzbekova, Svetlana, Thelie, Aurore, Delaleu, Bernadette, Mermillod, Pascal, Dalbies-Tran, Rozenn, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
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bovin ,folliculogénèse ,gène mater ,femelle ,expression génique ,développement embryonnaire ,embryon ,Biologie du développement ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Development Biology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2006
13. Development of an in vitro microenvironment for maturing oocytes
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Ihm, Jong Eun and Hubbell, Jeffrey A.
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folliculogénèse ,ligand c-Kit ,extracellular matrix ,matrice extracellulaire ,PEG-hydrogel ,c-Kit ligand ,follicle ,lymphangiogenesis ,VEGFR-3 ,FactorXIIIa ,FactorXIIIa substrate ,folliculogenesis ,c-Kit ,primordial follicle ,follicule ,follicule primordial ,Lymphangiogénèse - Abstract
The development of in vitro culture systems, comparable to the in vivo microenvironment in terms of effect on the oocyte growth and development could provide a valuable experimental tool for studying the mechanisms governing folliculogenesis. This tool might serve as well for practical clinical, agricultural, zoological, or biotechnological applications. This thesis reports on the importance of the microenvironment for the ovarian folliculogenesis process. The complexity of such a microenvironment was approached with a strategy based on functionalized PEG-hydrogels. The PEG matrix not only served as a scaffold, but it was also used a "reservoir" of immobilized cues. Tethered integrin-binding peptides in combination with other signaling factors aimed at better understanding the interactions of the oocyte and its surrounding granulosa cells that, most probably, determine the efficiency of the actual and the future in vitro mature oocyte production strategies. This work led also to investigating the lymph angiogenesis and the lymphatic transport in the context of oocyte maturation and their impact on mice fertility. In a first step, the mechanical properties of PEG-hydrogel were optimized for culturing secondary follicles. This report shows that the growth of the follicles was highly dependent on the mechanical properties of the surrounding environment. The optimal elastic modulus was found to be close to 900 Pa. In a second step, the effect of Arg-Gly-Asp (RGD) peptide, the minimal integrin-binding sequence, was studied. RGD presence did not influence the follicle growth rate but it significantly improved the quality of the produced oocytes. These findings demonstrated that approaching, biochemically and mechanically, the complexity of the ovarian extracellular matrix could be a winning strategy. The effect of key soluble factors was also investigated in order 1- to confirm their compatibility with the established 3-D culture system and 2- to further improve qualitatively and quantitatively the produced mature oocytes. Various combinations of gonadotropins such as the follicle-stimulating hormone (FSH) or the luteinizing hormone (LH) were tested. Interestingly, the effects of the gonadotropins in the 3-D PEG system were close to their known effects in vivo. Here the aberrant effects of these hormones in the used 2-D systems appeared clearly. c-Kit ligand (KL) is suspected to be one of the most important factors for the activation of primordial follicles and thus for controlling the exit from the resting pool. Previously, studying the effects of the two forms of KL (soluble and membrane-attached) had to cope with the lack of biologically-relevant immobilization strategies. For overcoming this problem, KL constructs were designed to include a substrate sequence for Factor XIIIa (NQEQVSPL or NQEQVSPLRCG). Thus the produced recombinant KL proteins could be enzymatically crosslinked to the PEG matrix. The different constructs of KL, including a wild type extracellular domain, were successfully cloned and produced from mammalian HEK-293 cells. The identity and the activity of the produced proteins were confirmed. Ovarian tissues from four days-old mice were cultured in PEG-hydrogels functionalized with KL. The results showed that the primordial follicles grew and were activated in the PEG-hydrogels where KL was immobilized but not when the soluble form of KL was preset in the medium. This experiment showed that the membrane-attached and the soluble form of KL play a different role in the rodent folliculogenesis. In parallel it was observed that blocking vascular endothelial growth factor receptor-3 (VEGFR-3) signaling had a critical but still unsuspected role in reproduction. This study demonstrates that variation in lymphangiogenesis is a regular, non-pathological event during folliculogenesis in the ovary; blocking lymphangiogenesis, might have an effect on hormone transport and thus on pregnancy. The reported results demonstrates that the blockade of lymphangiogenesis decreases the progesterone and estradioal levels during pregnancy and in fine results in failed fetal development. In conclusion, this study demonstrates the efficiency and the flexibility of a novel 3-D culture system. Circumventing problems inherent to the "on-plastic" standard culture, such as the loss of the granulosa-oocyte interactions, allowed the emergence of a culture system tailored for investigating fundamental folliculogenesis-related questions. Furthermore, the reported culture system might serve as a platform for developing clinical and biotechnology applications.
14. An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog
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Sylvain Foissac, Florent Woloszyn, Nathalie Marsaud, Olivier Bouchez, Agnès Bonnet, Philippe Mulsant, Beatrice Mandon-Pepin, Cédric Cabau, Julien Sarry, Laboratoire de Génétique Cellulaire (LGC), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), GeT PlaGe, Genotoul, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Biologie du Développement et Reproduction (BDR), This work was supported by an INRA 'Bioressources 2010' grant., Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Unité de Recherches Avicoles (URA), Génome et Transcriptome - Plateforme Génomique (GeT-PlaGe), Institut National de la Recherche Agronomique (INRA)-Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
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Granulosa cells ,Cell Communication ,Transcriptome ,0302 clinical medicine ,Ovarian Follicle ,microdissection ,rna-seq ,early folliculogenesis ,molecular dialog ,transcriptome ,sheep ,oocyte ,granulosa cell ,ovin ,Gene expression ,Biologie de la reproduction ,Cluster Analysis ,fonction biologique ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Laser capture microdissection ,Regulation of gene expression ,Genetics ,0303 health sciences ,Reproductive Biology ,030219 obstetrics & reproductive medicine ,folliculogénèse ,Wnt signaling pathway ,Biologie du développement ,Development Biology ,Cell biology ,medicine.anatomical_structure ,Organ Specificity ,interaction cellulaire ,Female ,Folliculogenesis ,Research Article ,Signal Transduction ,Biotechnology ,Cell signaling ,expression génique ,Biology ,03 medical and health sciences ,medicine ,Animals ,Humans ,Ovarian follicle ,régulateur de transcription ,030304 developmental biology ,ovaire ,Computational Biology ,Reproducibility of Results ,Molecular Sequence Annotation ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Gene Expression Regulation ,Oocytes ,Transcription Factors - Abstract
Chantier qualité GA; Background: Successful early folliculogenesis is crucial for female reproductive function. It requires appropriate gene specific expression of the different types of ovarian cells at different developmental stages. To date, most gene expression studies on the ovary were conducted in rodents and did not distinguish the type of cell. In mono-ovulating species, few studies have addressed gene expression profiles and mainly concerned human oocytes. Results: We used a laser capture microdissection method combined with RNA-seq technology to explore the transcriptome in oocytes and granulosa cells (GCs) during development of the sheep ovarian follicle. We first documented the expression profile of 15 349 genes, then focused on the 5 129 genes showing differential expression between oocytes and GCs. Enriched functional categories such as oocyte meiotic arrest and GC steroid synthesis reflect two distinct cell fates. We identified the implication of GC signal transduction pathways such as SHH, WNT and RHO GTPase. In addition, signaling pathways (VEGF, NOTCH, IGF1, etc.) and GC transzonal projections suggest the existence of complex cell-cell interactions. Finally, we highlighted several transcription regulators and specifically expressed genes that likely play an important role in early folliculogenesis. Conclusions: To our knowledge, this is the first comprehensive exploration of transcriptomes derived from in vivo oocytes and GCs at key stages in early follicular development in sheep. Collectively, our data advance our understanding of early folliculogenesis in mono-ovulating species and will be a valuable resource for unraveling human ovarian dysfunction such as premature ovarian failure (POF).
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