45 results on '"Folkow L"'
Search Results
2. Size and distribution of oxygen stores in harp and hooded seals from birth to maturity
- Author
-
Burns, J. M., Lestyk, K. C., Folkow, L. P., Hammill, M. O., and Blix, A. S.
- Published
- 2007
- Full Text
- View/download PDF
3. Apparent Seroprevalence of Salmonella spp. in Harp Seals in the Greenland Sea as Determined by Enzyme-linked Immunosorbent Assay
- Author
-
Aschfalk, A., Folkow, L., Rud, H., and Denzin, N.
- Published
- 2002
- Full Text
- View/download PDF
4. Simulations of the Effect of Currently Used Grenade Harpoons for the Killing of Whales Using a Pig-Model
- Author
-
Blix, A. S., Folkow, L. P., and Sørlie, D. G.
- Published
- 2000
- Full Text
- View/download PDF
5. On the direction and velocity of blood flow in the extradural intravertebral vein of harp seals (Phoca groenlandica) during simulated diving
- Author
-
NORDGARDEN, U., FOLKOW, L. P., WALLØE, L., and BLIX, A. S.
- Published
- 2000
6. Brain cooling in diving seals
- Author
-
ODDEN, Å, FOLKOW, L. P., CAPUTA, M., HOTVEDT, R., and BLIX, A. S.
- Published
- 1999
7. Blubber and flipper heat transfer in harp seals
- Author
-
KVADSHEIM, P. H. and FOLKOW, L. P.
- Published
- 1997
8. Influence of Snow Properties on Speed and Gait Choice in the Svalbard Rock Ptarmigan (Lagopus muta hyperborea).
- Author
-
Mármol-Guijarro, A, Nudds, R, Folkow, L, Sellers, W, Falkingham, P, and Codd, J
- Published
- 2021
- Full Text
- View/download PDF
9. The eyes of the deep diving hooded seal (Cystophora cristata) enhance sensitivity to ultraviolet light
- Author
-
Hogg, C., Neveu, M., Folkow, L., Stokkan, K-A., Kam, J. H., Douglas, R. H., and Jeffery, G.
- Subjects
genetic structures ,QH ,RE ,sense organs - Abstract
The mammalian visual range is approximately 400-700nm, although recent evidence suggests varying ultraviolet (UV) extensions in diverse terrestrial species. UV sensitivity may have advantages in the dim, blue light shifted environment experienced by submerged marine mammals. It may also be advantageous when seals are hauled out as UV is reflected by snow and ice but absorbed by fur, enhancing visual contrast. Here we show that the pelagic hooded seal (Cystophora cristata) has a highly UV permissive cornea and lens. Seals like other carnivores have a tapetum lucidum (TL) reflecting light back through the retina increasing sensitivity. The TL in this seal is unusual being white and covering almost the entire retina unlike that in other carnivores. Spectral reflectance from its surface selectively increases the relative UV/blue components >10 times than other wavelengths. Retinal architecture is consistent with a high degree of convergence. Enhanced UV from a large TL surface with a high degree of retinal convergence will increase sensitivity at a cost to acuity. UV electrophysiological retina responses were only obtained to dim, rod mediated stimuli, with no evidence of cone input. As physiological measurements of threshold sensitivity are log units higher than those for psychophysical detection, these seals are likely to be more UV sensitive than our results imply. Hence, UV reflections from the TL will afford increased sensitivity in dim oceanic environments.
- Published
- 2015
10. Estimating migration proportions from discretely observed continuous diffusion processes
- Author
-
Calian, V., Stefansson, G., Folkow, L. P., and Blix, A. S.
- Subjects
Methodology (stat.ME) ,FOS: Computer and information sciences ,FOS: Mathematics ,Mathematics - Statistics Theory ,Statistics Theory (math.ST) ,Statistics - Methodology - Abstract
We model two time and space scales discrete observations by using a unique continuous diffusion process with time dependent coefficient. We define new parameters for the large scale model as functions of the small scale distribution cumulants. We use the non - uniform distribution of the observation time intervals to obtain consistent and unbiased estimators for these parameters. Closed form expressions for migration proportions between spatial domains are derived as functions of these parameters. The models are applied to estimate migration patterns from satellite tag data., 25 pages, 5 figures
- Published
- 2009
11. Evidence for energy savings from aerial running in the Svalbard rock ptarmigan (Lagopus muta hyperborea).
- Author
-
Nudds, R. L., Folkow, L. P., Lees, J. J., Tickle, P. G., Stokkan, K.-A., and Codd, J. R.
- Subjects
- *
ROCK ptarmigan , *BIOMECHANICS , *CALORIC expenditure , *TREADMILLS , *ENERGY metabolism , *BIRD locomotion - Abstract
Svalbard rock ptarmigans were walked and run upon a treadmill and their energy expenditure measured using respirometry. The ptarmigan used three different gaits: a walking gait at slow speeds (less than or equal to 0.75 ms-1), grounded running at intermediate speeds (0.75 ms-1, U, 1.67 ms-1) and aerial running at high speeds (greater than or equal to 1.67 ms-1). Changes of gait were associated with reductions in the gross cost of transport (COT; J kg21 m21), providing the first evidence for energy savings with gait change in a small crouched-postured vertebrate. In addition, for the first time (excluding humans) a decrease in absolute metabolic energy expenditure (rate of O² consumption) in aerial running when compared with grounded running was identified. The COT versus U curve varies between species and the COT was cheaper during aerial running than grounded running, posing the question of why grounded running should be used at all. Existing explanations (e.g. stability during running over rocky terrain) amount to just so stories with no current evidence to support them. It may be that grounded running is just an artefact of treadmill studies. Research investigating the speeds used by animals in the field is sorely needed. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
12. The role of glycogen, glucose and lactate in neuronal activity during hypoxia in the hooded seal (Cystophora cristata) brain.
- Author
-
Czech-Damal, N. U., Geiseler, S. J., Hoff, M. L. M., Schliep, R., Ramirez, J.-M., Folkow, L. P., and Burmester, T.
- Subjects
- *
GLYCOGEN , *GLUCOSE , *HYPOXEMIA , *HOODED seal , *CEREBROSPINAL fluid , *CARBOHYDRATES - Abstract
The brains of diving mammals are repeatedly exposed to hypoxic conditions during diving. Brain neurons of the hooded seal (Cystophora cristata) have been shown to be more hypoxia tolerant than those of mice, but the underlying mechanisms are not clear. Here we investigated the roles of different metabolic substrates for maintenance of neuronal activity and integrity, by comparing the in vitro spontaneous neuronal activity of brain slices from layer V of the visual cortex of hooded seals with those in mice (Mus musculus). Studies were conducted by manipulating the composition of the artificial cerebrospinal fluid (aCSF), containing either 10 mM glucose, or 20 mM lactate, or no external carbohydrate supply (aglycemia). Normoxic, hypoxic and ischemic conditions were applied. The lack of glucose or the application of lactate in the aCSF containing no glucose had little effect on the neuronal activity of seal neurons in either normoxia or hypoxia, while neurons from mice survived in hypoxia only few minutes regardless of the composition of the aCSF. We propose that seal neurons have higher intrinsic energy stores. Indeed, we found about three times higher glycogen stores in the seal brain (~4.1 ng per µg total protein in the seal cerebrum) than in the mouse brain. Notably, in aCSF containing no glucose, seal neurons can tolerate 20 mM lactate while in mouse neuronal activity vanished after few minutes even in normoxia. This can be considered as an adaptation to long dives, during which lactate accumulates in the blood. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
13. The Influence of Snow Properties on Speed and Gait Choice in the Svalbard Rock Ptarmigan ( Lagopus muta hyperborea ).
- Author
-
Mármol-Guijarro A, Nudds R, Folkow L, Sellers W, Falkingham P, and Codd J
- Abstract
Substrate supportiveness is linked to the metabolic cost of locomotion, as it influences the depth to which the foot of a moving animal will sink. As track depth increases, animals typically reduce their speed to minimize any potential energetic imbalance. Here, we examine how self-selected speed in the Svalbard rock ptarmigan is affected by snow supportiveness and subsequent footprint depth measured using thin-blade penetrometry and 3D photogrammetry, respectively. Our findings indicate that snow supportiveness and footprint depth are poor predictors of speed ( r
2 = 0.149) and stride length ( r2 = 0.106). The ptarmigan in our study rarely sunk to depths beyond the intertarsal joint, regardless of the speed, suggesting that at this relatively shallow depth any increased cost is manageable. 3D reconstructions also indicate that the ptarmigan may exploit the compressive nature of snow to generate thrust during stance, as a trend toward greater foot rotations in deeper footprints was found. It remains unclear whether the Svalbard ptarmigan are deliberately avoiding unsupportive snowy substrates. However, if they do, these results would be consistent with the idea that animals should choose routes that minimize energy costs of locomotion., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology.)- Published
- 2021
- Full Text
- View/download PDF
14. Does posture explain the kinematic differences in a grounded running gait between male and female Svalbard rock ptarmigan ( Lagopus muta hyperborea ) moving on snow?
- Author
-
Marmol-Guijarro A, Nudds R, Folkow L, Lees J, and Codd J
- Abstract
The majority of locomotor research is conducted on treadmills and few studies attempt to understand the differences between this and animals moving in the wild. For example, animals may adjust their gait kinematics or limb posture, to a more compliant limb, to increase stability of locomotion to prevent limb failure or falling on different substrates. Here, using video recordings, we compared locomotor parameters (speed range, stride length, stride frequency, stance duration, swing duration and duty factor) of female Svalbard rock ptarmigan ( Lagopus muta hyperborea ) moving in the wild over snow to previous treadmill-based research. We also compared the absolute and body size (body mass and limb length)-corrected values of kinematic parameters to published data from males to look for any sex differences across walking and grounded running gaits. Our findings indicate that the kinematics of locomotion are largely conserved between the field and laboratory in that none of the female gaits were drastically affected by moving over snow, except for a prolonged swing phase at very slow walking speeds, likely due to toe dragging. Comparisons between the sexes indicate that the differences observed during a walking gait are likely due to body size. However, sexual dimorphism in body size could not explain the disparate grounded running kinematics of the female and male ptarmigan, which might be linked to a more crouched posture in females. Our findings provide insight into how males and females moving in situ may use different strategies to alleviate the effects of a variable substrate., Supplementary Information: The online version contains supplementary material available at 10.1007/s00300-021-02872-x., Competing Interests: Conflict of interestThe authors declare no competing interests., (© The Author(s) 2021.)
- Published
- 2021
- Full Text
- View/download PDF
15. Examining the accuracy of trackways for predicting gait selection and speed of locomotion.
- Author
-
Marmol-Guijarro A, Nudds R, Folkow L, and Codd J
- Abstract
Background: Using Froude numbers ( Fr ) and relative stride length (stride length: hip height), trackways have been widely used to determine the speed and gait of an animal. This approach, however, is limited by the ability to estimate hip height accurately and by the lack of information related to the substrate properties when the tracks were made, in particular for extinct fauna. By studying the Svalbard ptarmigan moving on snow, we assessed the accuracy of trackway predictions from a species-specific model and two additional Fr based models by ground truthing data extracted from videos as the tracks were being made., Results: The species-specific model accounted for more than 60% of the variability in speed for walking and aerial running, but only accounted for 19% when grounded running, likely due to its stabilizing role while moving faster over a changing substrate. The error in speed estimated was 0-35% for all gaits when using the species-specific model, whereas Fr based estimates produced errors up to 55%. The highest errors were associated with the walking gait. The transition between pendular to bouncing gaits fell close to the estimates using relative stride length described for other extant vertebrates. Conversely, the transition from grounded to aerial running appears to be species specific and highly dependent on posture and substrate., Conclusion: Altogether, this study highlights that using trackways to derive predictions on the locomotor speed and gait, using stride length as the only predictor, are problematic as accurate predictions require information from the animal in question., Competing Interests: Competing interestsThe authors declare that we have no competing interests., (© The Author(s) 2020.)
- Published
- 2020
- Full Text
- View/download PDF
16. A Comparative Morphometric Analysis of Three Cranial Nerves in Two Phocids: The Hooded Seal (Cystophora cristata) and the Harbor Seal (Phoca vitulina).
- Author
-
Wohlert D, Kröger J, Witt M, Schmitt O, Wree A, Czech-Damal N, Siebert U, Folkow L, and Hanke FD
- Subjects
- Animals, Female, Male, Cranial Nerves anatomy & histology, Phoca anatomy & histology
- Abstract
While our knowledge about the senses of pinnipeds has increased over the last decades almost nothing is known about the organization of the neuroanatomical pathways. In a first approach to this field of research, we assessed the total number of myelinated axons of three cranial nerves (CNs) in the harbor (Phoca vitulina, Pv) and hooded seal (Cystophora cristata, Cc). Axons were counted in semithin sections of the nerves embedded in Epon and stained with toluidine blue. In both species, the highest axon number was found within the optic nerve (Pv 187,000 ± 8,000 axons, Cc 481,600 ± 1,300 axons). Generally, considering absolute axon numbers, far more axons were counted within the optic and trigmenial nerve (Pv 136,700 ± 2,500 axons, Cc 179,300 ± 6,900 axons) in hooded in comparison to harbor seals. The axon counts of the vestibulocochlear nerve are nearly identical for both species (Pv 87,100 ± 8,100 axons, Cc 86,600 ± 2,700 axons). However, when comparing cell density, the cell density is almost equal for all nerves for both species except for the optic nerve in which cell density was particularly higher than in the other nerves and higher in hooded in comparison to harbor seals. We here present the first comparative analysis of three CNs in two phocid seals. While the CNs of these closely related species share some general characteristics, pronounced differences in axon numbers/densities are apparent. These differences seem to reflect differences in e.g. size, habitat, and/or functional significance of the innervated sensory systems., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
- Full Text
- View/download PDF
17. The eyes of the deep diving hooded seal (Cystophora cristata) enhance sensitivity to ultraviolet light.
- Author
-
Hogg C, Neveu M, Folkow L, Stokkan KA, Hoh Kam J, Douglas RH, and Jeffery G
- Abstract
The mammalian visual range is approximately 400-700 nm, although recent evidence suggests varying ultraviolet (UV) extensions in diverse terrestrial species. UV sensitivity may have advantages in the dim, blue light shifted environment experienced by submerged marine mammals. It may also be advantageous when seals are on land as UV is reflected by snow and ice but absorbed by fur, enhancing visual contrast. Here we show that the pelagic hooded seal (Cystophora cristata) has a highly UV permissive cornea and lens. Seals like other carnivores have a tapetum lucidum (TL) reflecting light back through the retina increasing sensitivity. The TL in this seal is unusual being white and covering almost the entire retina unlike that in other carnivores. Spectral reflectance from its surface selectively increases the relative UV/blue components >10 times than other wavelengths. Retinal architecture is consistent with a high degree of convergence. Enhanced UV from a large TL surface with a high degree of retinal convergence will increase sensitivity at a cost to acuity. UV electrophysiological retina responses were only obtained to dim, rod mediated stimuli, with no evidence of cone input. As physiological measurements of threshold sensitivity are much higher than those for psychophysical detection, these seals are likely to be more UV sensitive than our results imply. Hence, UV reflections from the TL will afford increased sensitivity in dim oceanic environments., (© 2015. Published by The Company of Biologists Ltd.)
- Published
- 2015
- Full Text
- View/download PDF
18. KATP-channels play a minor role in the protective hypoxic shut-down of cerebellar activity in eider ducks (Somateria mollissima).
- Author
-
Geiseler SJ, Ludvigsen S, and Folkow LP
- Subjects
- Animals, Cerebellum drug effects, Diazoxide pharmacology, Female, Hypoxia, Brain drug therapy, Male, Membrane Transport Modulators pharmacology, Microelectrodes, Neurons drug effects, Neuroprotective Agents pharmacology, Tissue Culture Techniques, Tolbutamide pharmacology, Avian Proteins metabolism, Cerebellum physiopathology, Ducks physiology, Hypoxia, Brain physiopathology, KATP Channels metabolism, Neurons physiology
- Abstract
Eider duck (Somateria mollissima) cerebellar neurons are highly tolerant toward hypoxia in vitro, which in part is due to a hypoxia-induced depression of their spontaneous activity. We have studied whether this response involves ATP-sensitive potassium (KATP) channels, which are known to be involved in the hypoxic/ischemic defense of mammalian neural and muscular tissues, by causing hyperpolarization and reduced ATP demand. Extracellular recordings in the Purkinje layer of isolated normoxic eider duck cerebellar slices showed that their spontaneous neuronal activity decreased significantly compared to in control slices when the KATP channel opener diazoxide (600 μM) was added (F1,70=92.781, p<0.001). Adding the KATP channel blocker tolbutamide (400 μM) 5 min prior to diazoxide completely abolished its effect (F1,55=39.639, p<0.001), strongly suggesting that these drugs have a similar mode of action in this avian species as in mammals. The spontaneous activity of slices treated with tolbutamide in combined hypoxia/chemical anoxia (95% N2-5% CO2 and 2 mM NaCN) was not significantly different from that of control slices (F1,203=0.071, p=0.791). Recovery from hypoxia/anoxia was, however, slightly but significantly weaker in tolbutamide-treated slices than in control slices (F1,137=15.539, p<0.001). We conclude that KATP channels are present in eider duck cerebellar neurons and are activated in hypoxia/anoxia, but that they do not play a key role in the protective shut-down response to hypoxia/anoxia., (Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
19. Shifting mirrors: adaptive changes in retinal reflections to winter darkness in Arctic reindeer.
- Author
-
Stokkan KA, Folkow L, Dukes J, Neveu M, Hogg C, Siefken S, Dakin SC, and Jeffery G
- Subjects
- Animals, Arctic Regions, Intraocular Pressure, Seasons, Adaptation, Physiological, Darkness, Reindeer physiology, Retina physiology, Vision, Ocular physiology
- Abstract
Arctic reindeer experience extreme changes in environmental light from continuous summer daylight to continuous winter darkness. Here, we show that they may have a unique mechanism to cope with winter darkness by changing the wavelength reflection from their tapetum lucidum (TL). In summer, it is golden with most light reflected back directly through the retina, whereas in winter it is deep blue with less light reflected out of the eye. The blue reflection in winter is associated with significantly increased retinal sensitivity compared with summer animals. The wavelength of reflection depends on TL collagen spacing, with reduced spacing resulting in shorter wavelengths, which we confirmed in summer and winter animals. Winter animals have significantly increased intra-ocular pressure, probably produced by permanent pupil dilation blocking ocular drainage. This may explain the collagen compression. The resulting shift to a blue reflection may scatter light through photoreceptors rather than directly reflecting it, resulting in elevated retinal sensitivity via increased photon capture. This is, to our knowledge, the first description of a retinal structural adaptation to seasonal changes in environmental light. Increased sensitivity occurs at the cost of reduced acuity, but may be an important adaptation in reindeer to detect moving predators in the dark Arctic winter.
- Published
- 2013
- Full Text
- View/download PDF
20. The metabolic cost of incline locomotion in the Svalbard rock ptarmigan (Lagopus muta hyperborea): the effects of incline grade and seasonal fluctuations in body mass.
- Author
-
Lees J, Folkow L, Stokkan KA, and Codd J
- Subjects
- Animals, Biomechanical Phenomena, Body Mass Index, Energy Metabolism, Male, Seasons, Galliformes physiology, Locomotion
- Abstract
In a terrestrial environment animals must locomote over varying terrain; despite this, the majority of studies of animal locomotion focus on level locomotion. The influence moving up an inclined surface has on the metabolic cost of locomotion and the efficiency with which animals perform positive work against gravity is still not well understood. Generally speaking, existing data sets lack consistency in the use of grades, further compounded by differences between species in terms of morphology and locomotor gait. Here we investigated the metabolic cost of locomotion using respirometry in the Svalbard ptarmigan (Lagopus muta hyperborea). The Svalbard ptarmigan provides a unique opportunity to investigate the cost of incline locomotion as it undergoes a seasonal fluctuation in body mass, which doubles in winter, meaning the requirement for positive mechanical work also fluctuates with season. We demonstrate that at the same degree of incline, the cost of lifting 1 kg by 1 vertical metre remains relatively constant between seasons despite the large differences in body mass from summer to winter. These findings are consistent with the notion that positive mechanical work alone dictates the cost of lifting above a certain body mass. However, our data indicate that this cost may vary according to the degree of incline and gait.
- Published
- 2013
- Full Text
- View/download PDF
21. Neuroglobin of seals and whales: evidence for a divergent role in the diving brain.
- Author
-
Schneuer M, Flachsbarth S, Czech-Damal NU, Folkow LP, Siebert U, and Burmester T
- Subjects
- Animals, Astrocytes metabolism, Brain anatomy & histology, Cattle, Cloning, Molecular, Dogs, Ferrets, Glial Fibrillary Acidic Protein metabolism, Globins genetics, Nerve Tissue Proteins genetics, Neuroglobin, RNA, Messenger metabolism, Seals, Earless metabolism, Sequence Analysis, Protein, Swine, Whales metabolism, Brain metabolism, Globins metabolism, Nerve Tissue Proteins metabolism, Seals, Earless anatomy & histology, Seals, Earless genetics, Whales anatomy & histology, Whales genetics
- Abstract
Although many physiological adaptations of diving mammals have been reported, little is known about how their brains sustain the high demands for metabolic energy and thus O(2) when submerged. A recent study revealed in the deep-diving hooded seal (Cystophora cristata) a unique shift of the oxidative energy metabolism and neuroglobin, a respiratory protein that is involved in neuronal hypoxia tolerance, from neurons to astrocytes. Here we have investigated neuroglobin in another pinniped species, the harp seal (Pagophilus groenlandicus), and in two cetaceans, the harbor porpoise (Phocoena phocoena) and the minke whale (Balaenoptera acutorostrata). Neuroglobin sequences, expression levels and patterns were compared with those of terrestrial relatives, the ferret (Mustela putorius furo) and the cattle (Bos taurus), respectively. Neuroglobin sequences of whales and seals only differ in two or three amino acids from those of cattle and ferret, and are unlikely to confer functional differences, e.g. in O(2) affinity. Neuroglobin is expressed in the astrocytes also of P. groenlandicus, suggesting that the shift of neuroglobin and oxidative metabolism is a common adaptation in the brains of deep-diving phocid seals. In the cetacean brain neuroglobin resides in neurons, like in terrestrial mammals. However, neuroglobin mRNA expression levels were 4-15 times higher in the brains of harbor porpoises and minke whales than in terrestrial mammals or in seals. Thus neuroglobin appears to play a specific role in diving mammals, but seals and whales have evolved divergent strategies to cope with cerebral hypoxia. The specific function of neuroglobin that conveys hypoxia tolerance may either relate to oxygen supply or protection from reactive oxygen species. The different strategies in seals and whales resulted from a divergent evolution and an independent adaptation to diving., (Copyright © 2012 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
22. Arctic reindeer extend their visual range into the ultraviolet.
- Author
-
Hogg C, Neveu M, Stokkan KA, Folkow L, Cottrill P, Douglas R, Hunt DM, and Jeffery G
- Subjects
- Amino Acid Sequence, Animals, Arctic Regions, Cornea physiology, Electroretinography veterinary, Lens, Crystalline anatomy & histology, Lens, Crystalline physiology, Male, Molecular Sequence Data, Norway, Opsins genetics, Retinal Cone Photoreceptor Cells physiology, Retinal Rod Photoreceptor Cells physiology, Seasons, Color Perception physiology, Cornea anatomy & histology, Reindeer physiology, Ultraviolet Rays
- Abstract
The Arctic has extreme seasonal changes in light levels and is proportionally UV-rich because of scattering of the shorter wavelengths and their reflection from snow and ice. Here we show that the cornea and lens in Arctic reindeer do not block all UV and that the retina responds electrophysiologically to these wavelengths. Both rod and cone photoreceptors respond to UV at low-intensity stimulation. Retinal RNA extraction and in vitro opsin expression show that the response to UV is not mediated by a specific UV photoreceptor mechanism. Reindeer thus extend their visual range into the short wavelengths characteristic of the winter environment and periods of extended twilight present in spring and autumn. A specific advantage of this short-wavelength vision is the use of potential information caused by differential UV reflections known to occur in both Arctic vegetation and different types of snow. UV is normally highly damaging to the retina, resulting in photoreceptor degeneration. Because such damage appears not to occur in these animals, they may have evolved retinal mechanisms protecting against extreme UV exposure present in the daylight found in the snow-covered late winter environment.
- Published
- 2011
- Full Text
- View/download PDF
23. Slow intrinsic oscillations in thick neocortical slices of hypoxia tolerant deep diving seals.
- Author
-
Ramirez JM, Folkow LP, Ludvigsen S, Ramirez PN, and Blix AS
- Subjects
- Animals, Diving adverse effects, Female, Hypoxia, Brain prevention & control, Organ Culture Techniques, Time Factors, Biological Clocks physiology, Brain Waves physiology, Diving physiology, Hypoxia, Brain physiopathology, Neocortex physiology, Seals, Earless physiology
- Abstract
Direct evidence that the mammalian neocortex is an important generator of intrinsic activity comes from isolated neocortical slices that spontaneously generate multiple rhythms including those in the beta, delta and gamma range. These oscillations are also seen in intact animals where they interact with other areas including the hippocampus, thalamus and basal ganglia. Here we show that thick isolated neocortical slices from hooded seals intrinsically generate persistent spontaneous activities, both repetitive non-rhythmic activity with activity states lasting for several minutes, and oscillating activity with rhythms that are much slower (<0.1 Hz) than the rhythms previously described in vitro. These intrinsic activities were very robust and persisted for up to 1 h even in severely hypoxic conditions. We hypothesize that the remarkable hypoxia tolerance of the hooded seal nervous system made it possible to maintain functional integrity in slices thick enough to preserve intact neuronal networks capable of generating these slow oscillations. The observed activities in seal neocortical slices support the notion that mammalian cortical networks intrinsically generate multiple states of activity that include oscillatory activity all the way down to <0.1 Hz. This intrinsic neocortical excitability is an important contributor not only to sleep but also to the default awake state of the neocortex., (Copyright © 2011 IBRO. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
24. Reduced metabolic cost of locomotion in Svalbard rock ptarmigan (Lagopus muta hyperborea) during winter.
- Author
-
Lees J, Nudds R, Stokkan KA, Folkow L, and Codd J
- Subjects
- Adaptation, Physiological, Analysis of Variance, Animal Migration, Animals, Arctic Regions, Biomechanical Phenomena, Body Weight, Carbon Dioxide metabolism, Energy Metabolism physiology, Fats metabolism, Male, Oxygen Consumption, Svalbard, Galliformes metabolism, Galliformes physiology, Locomotion physiology, Seasons
- Abstract
The Svalbard rock ptarmigan, Lagopus muta hyperborea experiences extreme photoperiodic and climatic conditions on the Arctic archipelago of Svalbard. This species, however, is highly adapted to live in this harsh environment. One of the most striking adaptations found in these birds is the deposition, prior to onset of winter, of fat stores which may comprise up to 32% of body mass and are located primarily around the sternum and abdominal region. This fat, while crucial to the birds' survival, also presents a challenge in that the bird must maintain normal physiological function with this additional mass. In particular these stores are likely to constrain the respiratory system, as the sternum and pelvic region must be moved during ventilation and carrying this extra load may also impact upon the energetic cost of locomotion. Here we demonstrate that winter birds have a reduced cost of locomotion when compared to summer birds. A remarkable finding given that during winter these birds have almost twice the body mass of those in summer. These results suggest that Svalbard ptarmigan are able to carry the additional winter fat without incurring any energetic cost. As energy conservation is paramount to these birds, minimising the costs of moving around when resources are limited would appear to be a key adaptation crucial for their survival in the barren Arctic environment.
- Published
- 2010
- Full Text
- View/download PDF
25. When the brain goes diving: glial oxidative metabolism may confer hypoxia tolerance to the seal brain.
- Author
-
Mitz SA, Reuss S, Folkow LP, Blix AS, Ramirez JM, Hankeln T, and Burmester T
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Animals, Astrocytes metabolism, Cerebellum metabolism, Female, Globins genetics, Hypoxia, Brain metabolism, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Molecular Sequence Data, Nerve Tissue Proteins genetics, Neuroglobin, Rats, Rats, Sprague-Dawley, Seals, Earless genetics, Sequence Homology, Amino Acid, Species Specificity, Brain metabolism, Cytochromes c metabolism, Globins metabolism, Nerve Tissue Proteins metabolism, Neuroglia metabolism, Neurons metabolism, Seals, Earless metabolism
- Abstract
Deep diving mammals have developed strategies to cope with limited oxygen availability when submerged. These adaptations are associated with an increased neuronal hypoxia tolerance. Brain neurons of the hooded seal Cystophora cristata remain much longer active in hypoxic conditions than those of mice. To understand the cellular basis of neuronal hypoxia tolerance, we studied neuroglobin and cytochrome c in C. cristata brain. Neuroglobin, a respiratory protein typically found in vertebrate neurons, displays three unique amino acid substitutions in hooded seal. However, these substitutions unlikely contribute to a modulation of O(2) affinity. Moreover, there is no significant difference in total neuroglobin protein levels in mouse, rat and seal brains. However, in terrestrial mammals neuroglobin resided exclusively in neurons, whereas in seals neuroglobin is mainly located in astrocytes. This unusual localization of neuroglobin is accompanied by a shift in the distribution of cytochrome c. In seals, this marker for oxidative metabolism is mainly localized in astrocytes, whereas in terrestrial mammals it is essentially found in neurons. Our results indicate that in seals aerobic ATP production depends significantly on astrocytes, while neurons rely less on aerobic energy metabolism. This adaptation may imbue seal neurons with an increased tolerance to hypoxia and potentially also to reactive oxygen species, and may explain in part the ability of deep diving mammals to sustain neuronal activity during prolonged dives.
- Published
- 2009
- Full Text
- View/download PDF
26. Where clocks are redundant: weak circadian mechanisms in reindeer living under polar photic conditions.
- Author
-
van Oort BE, Tyler NJ, Gerkema MP, Folkow L, and Stokkan KA
- Subjects
- Animals, Cold Climate, Darkness, Light, Norway, Animals, Wild, Circadian Rhythm, Periodicity, Reindeer physiology
- Abstract
Biological rhythms are a result of interplay between endogenous clocks and the ambient light-dark (LD) cycle. Biological timing in resident polar organisms presents a conundrum because these experience distinct daily LD cycles for only a few weeks each year. We measured locomotor activity in reindeer, Rangifer tarandus platyrhynchus (SR, n = 5 and 6) and R. tarandus tarandus (NR, n = 6), ranging freely at 78 and 70 degrees N, respectively, continuously throughout 1 year using data loggers. NR, but not SR, are gregarious which enabled us to examine the integrated effects of differences in social organisation and the photic environment at two different latitudes on the organisation of activity. In both sub-species, ultradian bouts of activity and inactivity alternated across the 24-h day throughout the year. This pattern was modified by the LD cycle in NR but barely at all in SR. Periodogram analysis revealed significant ultradian rhythmicity in both sub-species; the frequency of daily cycles of activity increased from three per day in winter to nearly five in summer. We conclude that this increase, and a concomitant increase in the level of daily activity, reflected the seasonal increase in the animals' appetite and the quality of their forage. Secondly, the combination, most evident in SR, of a weak photic response, weak circadian mechanisms and a weak social synchronization reduces the constraints of biological timing in an environment which is effectively non-rhythmic most of the year and permits expression of the basic ultradian pattern of ruminant activity. Third, the weaker 24-h rhythmicity in SR compared to NR indicates a latitudinal decrease in circadian organization and photic responsiveness in Rangifer.
- Published
- 2007
- Full Text
- View/download PDF
27. Circadian organization in reindeer.
- Author
-
van Oort BE, Tyler NJ, Gerkema MP, Folkow L, Blix AS, and Stokkan KA
- Subjects
- Animals, Arctic Regions, Behavior, Animal physiology, Behavior, Animal radiation effects, Biological Clocks physiology, Environment, Reindeer classification, Time Factors, Circadian Rhythm physiology, Circadian Rhythm radiation effects, Darkness, Reindeer physiology, Seasons, Sunlight
- Abstract
The light/dark cycle of day and night synchronizes an internal 'biological clock' that governs daily rhythms in behaviour, but this form of regulation is denied to polar animals for most of the year. Here we demonstrate that the continuous lighting conditions of summer and of winter at high latitudes cause a loss in daily rhythmic activity in reindeer living far above the Arctic Circle. This seasonal absence of circadian rhythmicity may be a ubiquitous trait among resident polar vertebrates.
- Published
- 2005
- Full Text
- View/download PDF
28. Endogenous glycogen prevents Ca2+ overload and hypercontracture in harp seal myocardial cells during simulated ischemia.
- Author
-
Henden T, Aasum E, Folkow L, Mjøs OD, Lathrop DA, and Larsen TS
- Subjects
- Adenosine Triphosphate metabolism, Animals, Cells, Cultured, Glucose metabolism, Glycolysis, Lactic Acid metabolism, Microscopy, Electron, Myocytes, Cardiac metabolism, Myocytes, Cardiac pathology, Oxygen metabolism, Rats, Rats, Sprague-Dawley, Seals, Earless, Species Specificity, Calcium metabolism, Glycogen metabolism, Ischemia
- Abstract
The purpose of this study was to determine if elevated myocardial glycogen content could obviate Ca(2+) overload and subsequent myocardial injury in the setting of low oxygen and diminished exogenous substrate supplies. Isolated harp seal cardiomyocytes, recognized as having large glycogen stores, were incubated under conditions simulating ischemia (oxygen and substrate deprivation) for 1 h. Rat cardiomyocytes were used for comparison. Freshly isolated seal cardiomyocytes contained approximately 10 times more glycogen than those from rats (479 +/- 39 vs. 48 +/- 5 nmol glucose/mg dry weight (dry wt), mean +/- S.E., n = 6), and during ischemia lactate production was significantly greater in seal compared to rat cardiomyocytes (660 +/- 99 vs. 97 +/- 14 nmol/mg dry wt), while glycogen content decreased both in seal (from 479 +/- 39 to 315 +/- 58 nmol glucose/mg dry wt) and rat cardiomyocytes (from 48 +/- 5 to 18 +/- 5 nmol glucose/mg dry wt). Cellular ATP was well maintained in ischemic seal cardiomyocytes, whereas it showed a 65% decline (from 31 +/- 3 to 11 +/- 1 nmol ATP/mg dry wt) in rat cardiomyocytes. Similarly, total seal cardiomyocyte Ca(2+) content was not affected by ischemia, while Ca(2+) increased from 8.5 +/- 2.0 to 13.3 +/- 2.0 nmol/mg dry wt in ischemic rat myocytes. Rat cardiomyocytes also showed a notable decline in the percentage of rod-shaped cells in response to ischemia (from 66 +/- 4% to 30 +/- 3%), and cell morphology was unaffected in seal incubations. Addition of iodoacetate (IAA, an inhibitor of glycolysis) to seal cardiomyocytes, on top of substrate and oxygen deprivation, reduced the cellular content of ATP by 52.9 +/- 4.4% (from 25 +/- 4 to 11 +/- 2 nmol ATP/mg dry wt) and the percentage of rod-shaped myocytes from 51 +/- 3% to 28 +/- 4%, while total Ca(2+) content was unchanged by these conditions. Seal cardiomyocytes thus tolerate low oxygen conditions better than rat cardiomyocytes. This finding is most likely due to a higher glycolysis rate in seals, fueled by larger myocardial glycogen stores.
- Published
- 2004
- Full Text
- View/download PDF
29. Cutaneous heat flux models do not reliably predict metabolic rates of marine mammals.
- Author
-
Boily P, Kvadsheim PH, and Folkow LP
- Subjects
- Animals, Seals, Earless metabolism, Skin Temperature physiology, Temperature, Body Temperature Regulation physiology, Models, Biological, Seals, Earless physiology, Skin Physiological Phenomena
- Abstract
Heat flux models have been used to predict metabolic rates of marine mammals, generally by estimating conductive heat transfer through their blubber layer. Recently, Kvadsheim et al. (1997) found that such models tend to overestimate metabolic rates, and that such errors probably result from the asymmetrical distribution of blubber. This problem may be avoided if reliable estimates of heat flux through the skin of the animals are obtained by using models that combine calculations of conductive heat flux through the skin and fur, and convective heat flux from the surface of the animal to the environment. We evaluated this approach based on simultaneous measurements of metabolic rates and of input parameters necessary for heat flux calculations, as obtained from four harp seals (Phoca groenlandica) resting in cold water. Heat flux estimates were made using two free convection models (double-flat-plate and cylindrical geometry) and one forced convection model (single-flat-plate geometry). We found that heat flux estimates generally underestimated metabolic rates, on average by 26-58%, and that small variations in input parameters caused large variations in these estimates. We conclude that cutaneous heat flux models are too inaccurate and sensitive to small errors in input parameters to provide reliable estimates of metabolic rates of marine mammals., (Copyright 2000 Academic Press.)
- Published
- 2000
- Full Text
- View/download PDF
30. Exostoses and cavernous venous formation in the external auditory canal of the hooded seal as a functional physiological organ.
- Author
-
Stenfors LE, Sade J, Hellström S, Anniko M, and Folkow L
- Subjects
- Animals, Animals, Newborn, Ear Neoplasms blood supply, Ear Neoplasms pathology, Female, Male, Osteochondroma blood supply, Sex Characteristics, Ear Canal blood supply, Ear Canal pathology, Ear Neoplasms veterinary, Osteochondroma veterinary, Seals, Earless
- Abstract
Exostoses of the external auditory canal (EAC) develop after protracted mechanical, chemical or thermal irritation in particular. This is a common disorder among aquatic sportsmen and has been considered unique to Man. We dissected and photodocumented the EACs of 5 newborn and 3 adult Hooded Seals (Cystophora cristata). Serial sections of the EACs were prepared for light microscopic evaluation after staining with haematoxylin-eosin or toluidine blue. All EACs exhibited a firm, broad-based. mountain peak-shaped exostosis on the floor of the meatus, lateral to the eardrum. In addition, the meatal skin of the bony EAC harboured large venous sinuses. The exostosis and venous sinuses of the seal EAC participate in the protection of the sensitive hearing apparatus, particularly the pars tensa portion of the drum, during divine.
- Published
- 2000
- Full Text
- View/download PDF
31. Panting in reindeer (Rangifer tarandus).
- Author
-
Aas-Hansen O, Folkow LP, and Blix AS
- Subjects
- Animals, Brain physiology, Cold Climate, Female, Models, Biological, Mouth, Norway, Nose, Seasons, Temperature, Tidal Volume, Body Temperature Regulation physiology, Reindeer physiology, Respiratory Mechanics physiology
- Abstract
Two winter-insulated Norwegian reindeer (Rangifer tarandus tarandus) were exposed to air temperatures of 10, 20, 30, and 38 degrees C while standing at rest in a climatic chamber. The direction of airflow through nose and mouth, and the total and the nasal minute volumes, respectively, were determined during both closed- and open-mouth panting. The animals alternated between closed- and open-mouth panting, but the proportion of open-mouth panting increased with increasing heat load. The shifts from closed- to open-mouth panting were abrupt and always associated with a rise in respiratory frequency and respiratory minute volume. During open-mouth panting, the direction of airflow was bidirectional in both nose and mouth, but only 2.4 +/- (SD) 1.1% of the air was routed through the nose. Estimates suggest that the potential for selective brain cooling is markedly reduced during open-mouth panting in reindeer as a consequence of this airflow pattern.
- Published
- 2000
- Full Text
- View/download PDF
32. Rapid brain cooling in diving ducks.
- Author
-
Caputa M, Folkow L, and Blix AS
- Subjects
- Animals, Asphyxia, Body Temperature, Cloaca, Female, Heart Rate, Male, Time Factors, Body Temperature Regulation, Brain physiology, Diving physiology, Ducks physiology, Hypothalamus physiology
- Abstract
Hypothermia may limit asphyxic damages to the brain, and many small homeotherms have been shown to use anapyrexic strategies when exposed to asphyxic conditions. Larger homeotherms do not seem to use the same strategy, but could save oxygen and prevent hypoxic brain damage by employing selective brain cooling (SBC) in connection with asphyxia. To test the hypothesis that selective brain cooling may take place in connection with asphyxia, we have recorded brain [hypothalamic (THyp)] and body [colonic (TC)] temperatures and heart rates in four Pekin ducks during 5-min simulated (head submersion) diving in cold water (10 degrees C). Diving resulted in a drop in THyp (3.1 +/- 1.4 degrees C) that continued into the recovery period (P < 0.001). Restricting heat loss from the buccal cavity and eyes during diving compromised brain cooling in an additive manner. TC was not influenced by diving. Control cooling of the head with crushed ice during a 5-min period of undisturbed breathing had no effect on THyp. Warm water (35 degrees C) markedly reduced brain cooling, and dive capacity was reduced by approximately 14% (P < 0.05) compared with diving in water at 10 degrees C. The data suggest that SBC is used in ducks during diving, and we propose that this mechanism may enable the bird to save oxygen for prolonged aerobic submergence and to protect the brain from asphyxic damages.
- Published
- 1998
- Full Text
- View/download PDF
33. Changes in fibrinolytic activity in diving grey seals.
- Author
-
Lohman S, Folkow LP, Osterud B, and Sager G
- Subjects
- Animals, Blood Coagulation physiology, Epinephrine blood, Female, Heart Rate physiology, Hydrocortisone blood, Norepinephrine blood, Time Factors, Diving physiology, Fibrinolysis physiology, Seals, Earless blood
- Abstract
In order to test the hypothesis that enhanced fibrinolytic activity is a factor which prevents the blood of diving seals from clotting, we instrumented two female grey seals (Halichoerus grypus) with subcutaneous electrodes for measurements of heart rate (HR) and an extradural intravertebral venous catheter for collection of blood samples before, during and after simulated dives of 10 min duration. Blood samples were used for in vitro determination of clot lysis time (CLT), which is a measure of the level of fibrinolytic activity, and for analyses of plasma levels of cortisol, noradrenaline and adrenaline (A). The seals displayed profound diving bradycardia indicative of a substantial reduction in blood flow rates (pre-dive HR: 78 (63-98) bpm; dive HR: 8 (7-10) bpm; (median (range); n = 2)) and elevated catecholamine levels (pre-dive A: 121 (98-184) pg.ml-1; peak dive/post-dive A: 3510 (447-6181) pg.ml-1), both of which are factors which promote blood coagulation. Nevertheless, we found that CLT always increased in connection with diving (pre-dive CLT: 436 (356-568) min; peak CLT during diving: 1380 (640-1800) min), which implies a reduced, rather than enhanced, fibrinolytic activity in this situation. These results show that enhanced fibrinolytic activity is not part of the defence system which prevents fatal clotting from occurring in diving grey seals.
- Published
- 1998
- Full Text
- View/download PDF
34. Effects of adrenergic and cholinergic drugs on splenic arteries and veins from hooded seals (Cystophora cristata).
- Author
-
Cabanac A, Folkow LP, and Blix AS
- Subjects
- Animals, Female, Male, Vascular Resistance drug effects, Adrenergic Agents pharmacology, Cholinergic Agents pharmacology, Seals, Earless physiology, Splenic Artery drug effects, Splenic Vein drug effects
- Abstract
Isolated ring preparations of arteries and veins from hooded seal spleens were subjected in vitro to adrenaline (A), noradrenaline (NA), isoprenaline (Iso), and acetylcholine (ACh), alone or in combination with the blockers phentolamine (Phe), propranolol (Pro), and atropine (Atr). Both arteries and veins constricted in response to A (the estimated effective dose required for half-maximal response (ED50) was 3.3 and 0.2 microM, for arteries and veins, respectively) and NA (estimated ED50 was 1.5 and 0.6 microM, for arteries and veins, respectively), but these effects were abolished when the drugs were given in combination with the alpha-adrenoceptor blocker Phe. The responses of arteries and veins to ACh and the beta-adrenoceptor agonist Iso were minor and inconsistent, and were completely abolished when combined with their respective blockers (Atr and Pro, respectively). The ED50 for both A and NA are quite high in relation to normal plasma levels of A and NA in seals. This implies that these vessels (and, hence, the supply of blood to the spleen) primarily are subjected to neurogenic, rather than humoral physiological control.
- Published
- 1998
- Full Text
- View/download PDF
35. Volume capacity and contraction control of the seal spleen.
- Author
-
Cabanac A, Folkow LP, and Blix AS
- Subjects
- Animals, Arteries, Diving physiology, Epinephrine pharmacology, Female, Hematocrit, In Vitro Techniques, Male, Receptors, Adrenergic, alpha drug effects, Receptors, Adrenergic, alpha physiology, Regional Blood Flow, Seals, Earless blood, Spleen blood supply, Spleen drug effects, Vasoconstriction, Veins physiology, Blood Volume, Seals, Earless physiology, Spleen physiology
- Abstract
Volume changes in the spleens of hooded seals (Cystophora cristata) and harp seals (Phoca groenlandica) were measured plethysmographically in vitro in response to epinephrine, norepinephrine, isoprenaline, phentolamine, and acetylcholine. Dilated spleens contracted forcefully within 1-3 min of alpha-adrenoceptor activation with 1.0-5.0 micrograms epinephrine/kg body mass, whereas stimulation of beta-adrenoceptors and cholinergic receptors had little effect. The mass of dilated hooded seal spleens corresponded to 2-4% (n = 7) of body mass, with volume (V; ml) relating to body mass (M; kg) as follows: V = 12.0M + 910 (r2 = 0.96, n = 4). Thus the spleen of a 250-kg hooded seal maximally expels 3.9 liters, or 13%, of its estimated total blood volume. Average hematocrit in splenic venous outflow from dilated spleens was 90 +/- 3% (n = 3) in hooded seals and 85% (n = 2) in harp seals. From these data we have estimated that the aerobic diving limit of a 250-kg hooded seal increases only 105 s, at the most, if complete emptying of the spleen occurs during diving, while the corresponding estimate for a 112-kg harp seal is 80 s.
- Published
- 1997
- Full Text
- View/download PDF
36. Pineal and thyroid functions in newborn seals.
- Author
-
Stokkan KA, Vaughan MK, Reiter RJ, Folkow LP, Mårtensson PE, Sager G, Lydersen C, and Blix AS
- Subjects
- Aging, Animals, Female, Male, Melatonin blood, Melatonin metabolism, Thyroxine blood, Triiodothyronine blood, Animals, Newborn physiology, Pineal Gland physiology, Seals, Earless physiology, Thyroid Gland physiology
- Abstract
Daily variations of pineal and plasma melatonin and plasma thyroid hormones were measured in harp seals (Phoca groenlandica), grey seals (Halichoerus grypus), and hooded seals (Cystophora cristata), ranging in age from newborn to 14 days. In newborn harp seals the mean mass of the pineal gland was 273 mg (+/- 45 SEM, n = 11), containing 49 ng (median) melatonin. In newborn, 4- and 10-day-old grey seals, the pineal mass was similar, weighing on average 337 mg (+/- 74, n = 6) and containing 90 ng melatonin. Two newborn hooded seal pups had pineals weighing 520 and 1289 mg, with 254 and 7600 ng melatonin, respectively. There were no day-night differences in the pineal contents of melatonin or in the number of pineal beta-adrenergic receptors measured in newborn harp seals, and, in newborn, 4- and 10-day-old grey seals, there were no day-night or age differences in pineal melatonin content. Plasma melatonin levels were 10 times higher in newborn seals than in two 10-day-old grey seals and one 14-day-old harp seal pup. In all seal pups, the levels exhibited a 24-hr rhythmicity, with increasing night- and decreasing daytime concentrations. Plasma levels of thyroxine (T4) and triiodothyronine (T3) were generally higher in newborn seals than in 10- and 14-day-old seals or in adult females. There was no apparent 24-hr rhythmicity, but the thyroid hormone levels generally declined throughout each sampling sequence. High pineal and thyroid activities may play a thermoregulatory role in newborn seals, but the results do not indicate a stimulatory action of melatonin in the peripheral conversion of T4 to T3. It is speculated that the large and active pineal gland, particularly in newborn seals, may be related to aspects of their diving habit.
- Published
- 1995
- Full Text
- View/download PDF
37. Daily energy expenditure in free living minke whales.
- Author
-
Blix AS and Folkow LP
- Subjects
- Animals, Eating, Kinetics, Norway, Sleep, Time Factors, Energy Metabolism physiology, Respiration physiology, Whales physiology
- Abstract
Six minke whales (Balaenoptera acutorostrata) were instrumented with VHF-radio transmitters and four with sonic speed-depth transmitters off the west coast of northern Norway and Svalbard and followed within view for up to 24 h. During such periods their respiratory rate was continuously recorded and their energy expenditure estimated according to Folkow & Blix (1992) at different swimming speeds and types of activity. We found that cost of swimming is remarkably low in these large animals and that their estimated daily energy expenditure on average only amounts to 80 kJ kg-1 day-1.
- Published
- 1995
- Full Text
- View/download PDF
38. Nasal heat and water exchange is not an effector mechanism for water balance regulation in grey seals.
- Author
-
Skog EB and Folkow LP
- Subjects
- Administration, Oral, Animals, Arginine Vasopressin blood, Body Water metabolism, Body Weight, Diuresis, Female, Food Deprivation, Injections, Intravenous, Mannitol pharmacology, Osmolar Concentration, Water administration & dosage, Water Deprivation, Water-Electrolyte Balance drug effects, Body Temperature Regulation physiology, Respiration physiology, Seals, Earless physiology, Water-Electrolyte Balance physiology
- Abstract
Phocid seals may effectively restrict respiratory heat and water loss by nasal heat and water exchange (NHE), and respiratory heat loss is, in fact, subject to thermoregulatory control. We have investigated whether phocid seals also control NHE and respiratory water loss to regulate water balance. Three resting juvenile female grey seals (Halichoerus grypus) were subjected to: (i) 5 days of food and water deprivation, (ii) intravenous infusion of 1000 ml of a hypersomotic (930 mM) solution of the diuretic mannitol, and (iii) oral injection of 1500 ml distilled water. During these experiments in air of 0 degree C, expired air temperature (T(ex)) and respiratory frequency (f) were recorded, and urine and blood samples collected. The results were compared with results from control experiments. Five days of food and water deprivation caused an average 10.5% and 20.8% increase in plasma (PO) and urine (UO) osmolality, respectively. Mannitol infusion induced excessive diuresis and caused an average 2.45% reduction of the estimated body water pool. Water loading caused an average 4.5% and 60% reduction in PO and UO, respectively, while urine production increased by 365%, on average. However, in no case did either T(ex) or f change significantly from mean control levels of 22.4 (range: 20.7-25.2) degrees C and 7.3 (range: 6.6-8.4) breaths min-1, respectively. Thus, water balance disturbances that initiate renal compensatory mechanisms fail to affect NHE in grey seals. This suggests that control of NHE is not an effector mechanism for regulation of water balance in grey seals.
- Published
- 1994
- Full Text
- View/download PDF
39. Adrenergic vasomotor responses in nasal mucosa of hooded seals.
- Author
-
Folkow LP
- Subjects
- Animals, Blood Vessels drug effects, Epinephrine pharmacology, Nasal Mucosa blood supply, Nasal Mucosa innervation, Norepinephrine pharmacology, Sympathomimetics pharmacology, Vascular Resistance drug effects, Nasal Mucosa physiology, Seals, Earless physiology, Sympathetic Nervous System physiology, Vasomotor System physiology
- Abstract
In seals respiratory heat and water losses are restricted through nasal heat exchange. The heat exchange efficiency is apparently controlled through adjustments in the nasal mucosal blood flow rate and/or pattern. In this study the adrenergic mechanisms involved in regulation of mucosal blood flow were investigated. The nasal mucosal vasculature of 14 newly killed hooded seal (Cystophora cristata) pups was perfused by a constant-flow peristaltic pump with 37 degrees C oxygenated modified Krebs solution via the sphenopalatine arteries. The effects of single-dose injections of various drugs on resistance to flow were monitored with a pressure transducer. Epinephrine, norepinephrine, alpha 1-adrenoceptor agonist phenylephrine, alpha 2-agonist clonidine, beta 1-agonist dobutamine, and beta 2-agonist terbutaline caused transient pressure increases that were blocked by alpha-adrenoceptor antagonists. Papaverine and vasoactive intestinal polypeptide induced vasodilatation, showing that some basal vascular tone was present. Nevertheless, the beta 1- and beta 2-agonist isoproterenol had no effect on resistance, and none of the beta-agonists attenuated the pressor responses to alpha-agonists. In conclusion, adrenergic control of nasal mucosal blood flow in seals is essentially exerted through alpha-adrenoceptor-mediated arteriolar constriction, whereas beta-adrenoceptor-mediated dilatation seems to be of little importance. It is suggested that such sympathoadrenergic vascular mechanisms contribute to control nasal heat exchange efficiency in seals.
- Published
- 1992
- Full Text
- View/download PDF
40. Metabolic rates of minke whales (Balaenoptera acutorostrata) in cold water.
- Author
-
Folkow LP and Blix AS
- Subjects
- Adipose Tissue anatomy & histology, Adipose Tissue physiology, Animals, Body Surface Area, Body Temperature Regulation physiology, Body Weight, Electric Conductivity, Energy Metabolism, Lung Volume Measurements, Temperature, Tidal Volume, Cold Temperature, Whales metabolism
- Abstract
Body temperature, blubber thickness and lung capacity (Vc) were recorded in newly killed minke whales, while respiratory frequency (f) was determined in free-swimming animals. Mean deep (thoracic) body temperature was 34.7 +/- 0.8 (SD) degrees C (n = 14). Weighted mean core/blubber interface temperature in animals caught in 2.5-5.5 degrees C water was 28.8 +/- 1.7 degrees C (n = 8). The minimum average rate of sensible heat loss (HLs) was 3.81 +/- 0.53 (SD) W kgw-0.75 (n = 8) in animals with body masses (w) in the range of 1840 to 5740 kg, HLs being inversely proportional to w (HLs = -2.98 10(-4) w +4.89 W kgw-0.75 (n = 8, r2 = 0.73, P less than 0.01)). The average rate of respiratory heat loss (HLr) was 0.26 +/- 0.04 (SD) W kgw-0.75, regardless of w, in the same 8 animals. Total rates of heat loss (HL = HLr+HLs) in 2.5-5.5 degrees C water ranged between 3.40 and 4.87 W kgw-0.75, with an average of 4.06 +/- 0.52 (SD) W kgw-0.75 (n = 8). Estimates of oxygen consumption based on records of f and Ve, and data on oxygen extraction from other cetaceans, yielded a range of metabolic rates which compared nicely with the calculated HL values.
- Published
- 1992
- Full Text
- View/download PDF
41. Distribution and vasomotor effects of neuropeptides in angular oculi and facial veins of reindeer.
- Author
-
Johnson HK, Helle KB, Lundberg JM, Hökfelt T, and Folkow LP
- Subjects
- Animals, Fluorescent Antibody Technique, In Vitro Techniques, Temperature, Veins physiology, Eye blood supply, Face blood supply, Neuropeptides metabolism, Reindeer physiology, Vasomotor System physiology
- Abstract
1. The vasomotor responses to neuropeptides of the angular oculi and facial veins of reindeer were examined in vitro and correlated with the neuropeptide distribution in the perivascular nerves, as demonstrated by immunohistochemistry. 2. Nerves displaying calcitonin gene related peptide (CGRP)- or neuropeptide Y (NPY)-like immunoreactivity (-LI) were observed in the media of both veins, while very few fibers were immunoreactive to vasoactive intestinal polypeptide (VIP) or substance P (SP) in either vein. 3. The staining pattern for NPY-LI was largely identical to that of dopamine-beta-hydroxylase, a marker for noradrenaline (NA) producing fibers, indicating coexistence of NPY and NA. 4. Administration of NPY in vitro elicited contractions in both veins in the presence of propranolol, though more conspicuously in the angular oculi vein. 5. The peptide was without any modulating effect on NA-stimulated contractions in the angular oculi vein, whereas a small enhancement of the NA-induced tone was seen in the facial vein. 6. CGRP caused partial relaxation of both veins, whereas atrial natriuretic polypeptide caused relaxation only in the facial vein. VIP and SP had no effect on either vein. 7. The results suggest that in reindeer the sympathetic nerve fibres to both facial and angular oculi veins contain the vasoconstrictor neuropeptide NPY besides NA, even though these fibres exert a vasodilator action on the myogenically active facial vein. 8. The vasodilator neuropeptide CGRP, which is present in other more sparse perivascular nerve fibres mainly in angular oculi vein, is perhaps of afferent nature in which case CGRP might subserve axon reflex functions. 9. If, however, also the CGRP fibres are truly efferent in nature, chances for a central reciprocal control of flow through angular oculi vein might be at hand.
- Published
- 1992
- Full Text
- View/download PDF
42. Partition of heat loss in resting and exercising winter- and summer-insulated reindeer.
- Author
-
Folkow LP and Mercer JB
- Subjects
- Animals, Basal Metabolism, Mathematics, Body Temperature Regulation, Physical Exertion, Reindeer physiology, Seasons
- Abstract
Partition of heat loss was performed in resting (standing) and exercising (running; 9.2 km . h-1 for 40 min) winter- (W) and summer- (S) insulated conscious reindeer (Rangifer tarandus tarandus) in a climatic chamber set to an ambient temperature (Ta) of -30, 0, or 25 degrees C. Heat loss and production were determined using indirect calorimetry. The relative importance of radiant heat loss was greatest during rest at low Ta's, both during winter and summer (44-58% at Ta's -30 and 0 degrees C). At high Ta's, especially during exercise, a large proportion of total heat loss occurred through evaporation [45% at Ta 25 degrees C during rest and 47% at Ta 0 degrees C during exercise (W); 38% at Ta 25 degrees C during exercise (S)]. The proportion of heat lost through convection during rest and exercise was 38-52 and 46-72%, respectively, depending on Ta and season. The legs were found to represent an important avenue for radiant heat loss, particularly in heat stress situations. Their importance for convective heat loss was even greater, especially during exercise.
- Published
- 1986
- Full Text
- View/download PDF
43. Vascular control of brain cooling in reindeer.
- Author
-
Johnson HK and Folkow LP
- Subjects
- Animals, Electric Stimulation, Face blood supply, Norepinephrine pharmacology, Nose blood supply, Receptors, Adrenergic, alpha physiology, Reference Values, Stress, Physiological physiopathology, Sympathetic Nervous System physiology, Vasoconstriction drug effects, Vasodilation, Body Temperature Regulation, Cerebrovascular Circulation, Reindeer physiology
- Abstract
The cold venous effluent returning from the nose of the heat-stressed reindeer may be distributed through angular oculi veins for selective cooling of the brain and through facial veins for general body cooling. In vitro experiments indicate that adrenergic receptors of the angular oculi vein are exclusively of the alpha-adrenergic type, whereas facial veins contain mainly beta-adrenergic receptors. We suggest that the antagonistic adrenergic neuroeffector organization of these veins plays a major role in control of brain cooling. Thus simultaneous sympathetic stimulation results in constriction of angular oculi veins and release of a preexisting stretch-induced tone in facial veins. In this situation cold venous blood is directed via facial veins and used for general body cooling. Reduction of sympathetic activity, on the other hand, results in dilatation of angular oculi veins and constriction of facial veins, due to development of myogenic tone in the latter vessels. In this situation cold venous blood is distributed via angular oculi veins and used for selective cooling of the brain.
- Published
- 1988
- Full Text
- View/download PDF
44. Nasal heat and water exchange in gray seals.
- Author
-
Folkow LP and Blix AS
- Subjects
- Algorithms, Animals, Basal Metabolism, Female, Male, Oxygen Consumption, Reference Values, Body Temperature Regulation, Body Water metabolism, Caniformia physiology, Nose, Seals, Earless physiology
- Abstract
Metabolic rate (MR), expired air temperature (Tex), respiratory frequency (f), respiratory minute volume (V), and skin (Ts) and body (Tb) temperatures were recorded in three gray seals (Halichoerus grypus) at ambient air temperatures (Ta) between -40 and +20 degrees C. At Ta within the thermoneutral zone, MR averaged 3.7 W.kg-0.75, while mean V was 0.26 1.min-1.kg-0.75. At Ta below -11 degrees C [apparent lower critical temperature (Tlc)], both MR and V increased linearly with decreasing Ta. Average maximum MR (9.6 W.kg-0.75) and V (0.57 1.min-1.kg-0.75) were both recorded at Ta of -40 degrees C. Tex decreased with decreasing Ta to an average minimum value of 8 degrees C at Ta of -30 degrees C. The highest Tex recorded was 32 degrees C at Ta of +20 degrees C. At Ta of -20 degrees C, both total respiratory heat loss, with one exception, and respiratory evaporative water loss reached their lowest values. At this Ta, 66% of the heat and 80% of the water added to the inspired air were regained on expiration. We suggest that nasal heat exchange may be of considerable importance for thermal and water balance in many pinnipeds.
- Published
- 1987
- Full Text
- View/download PDF
45. Thermoregulatory control of expired air temperature in diving harp seals.
- Author
-
Folkow LP and Blix AS
- Subjects
- Animals, Diving, Energy Metabolism, Female, Male, Temperature, Body Temperature Regulation, Caniformia physiology, Respiration, Seals, Earless physiology
- Abstract
Expired air temperature (Tex), metabolic rate (MR), and skin (Ts) and body (Tb; rectal) temperatures were recorded in four or five young (1-2 yr) harp seals (Phoca groenlandica) in air [mean air temperature (Ta) = -30, -10, or 10 degrees C] and in water [mean water temperature (Tw) = 2.3 or 24.8 degrees C, with Ta = -30, -10, 0, or 10 degrees C]. Apparent lower critical temperature was below -10 degrees C in air. Above this Ta, mean MR was 5.85 W.kg-0.75 (2.23 W.kg-1), while mean MR was 12.56 W.kg-0.75 (4.69 W.kg-1) at Ta -30 degrees C. When seals were immersed in water of 2.3 degrees C, mean MR was 6.13 W.kg-0.75 (2.31 W.kg-1), regardless of Ta. At Ta -30, -10, and 10 degrees C, mean Tex in air were 9.5, 13.0, and 25.0 degrees C, respectively. The corresponding values for seals in water (Tw = 2.3 degrees C) were 8.0, 9.5, and 15.5 degrees C, respectively. The low Tex recorded at Ta -30 and -10 degrees C in air and at all Ta in water (Tw = 2.3 degrees C) suggests that heat was conserved by nasal heat exchange. At Ta 10 degrees C, mean Tex of seals in air was approximately 10 degrees C higher than mean Tex of seals in water (Tw = 2.3 degrees C). Furthermore, seals subjected to a Tw of 24.8 degrees C at Ta 0 degrees C had a mean Tex 10 degrees C higher than when subjected to Tw 2.3 degrees C at the same Ta. These observations suggest that Tex in seals is under thermoregulatory control. In a series of forced dives of up to 5-min duration Tex was found to be the same before and after the dive regardless of dive duration and Ta.
- Published
- 1989
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.