47 results on '"Ferrante, Patrizia"'
Search Results
2. Severe COVID-19 pneumonia in Piacenza, Italy — A cohort study of the first pandemic wave
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Guglielmetti, Lorenzo, Kontsevaya, Irina, Leoni, Maria C., Ferrante, Patrizia, Fronti, Elisa, Gerna, Laura, Valdatta, Caterina, Donisi, Alessandra, Faggi, Alberto, Paolillo, Franco, Ratti, Giovanna, Ruggieri, Alessandro, Scotti, Marta, Sacchini, Daria, Taliani, Gloria, and Codeluppi, Mauro
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- 2021
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3. Treatment for COVID-19—a cohort study from Northern Italy
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Guglielmetti, Lorenzo, Aschieri, Daniela, Kontsevaya, Irina, Calabrese, Francesco, Donisi, Alessandra, Faggi, Alberto, Ferrante, Patrizia, Fronti, Elisa, Gerna, Laura, Leoni, Maria Cristina, Paolillo, Franco, Ratti, Giovanna, Ruggieri, Alessandro, Sacchini, Daria, Scotti, Marta, Valdatta, Caterina, Stabile, Marco, Taliani, Gloria, and Codeluppi, Mauro
- Published
- 2021
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4. Old or new occupants of energy rehabilitated buildings. Two different approaches for hierarchizing group of buildings
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Ferrante, Patrizia, Peri, Giorgia, Rizzo, Gianfranco, Scaccianoce, Gianluca, and Vaccaro, Valentina
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- 2017
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5. Vegetation growth parameters and leaf temperature: Experimental results from a six plots green roofs' system
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Ferrante, Patrizia, La Gennusa, Maria, Peri, Giorgia, Rizzo, Gianfranco, and Scaccianoce, Gianluca
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- 2016
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6. Visually low-impacting methods for the measurement of parameters related to IAQ risk indicators in exhibition halls
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Cirrincione Laura, Ferrante Patrizia, La Gennusa Maria, Peri Giorgia, Rizzo Gianfranco, and Scaccianoce Gianluca
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museums ,indoor air quality ,off-line sensors ,low visual impact ,corrosion risk ,artefact preservation ,visitors ‘comfort ,Environmental sciences ,GE1-350 - Abstract
Microclimate for cultural heritage plays a fundamental role in the degradation process of artefacts exhibited in museums. Generally, the Indoor Air Quality (IAQ) monitoring inside museums is based on conventional measurements of thermo-hygrometry parameters and longtime averaged measurements of atmospheric pollutants’ concentration. Unfortunately, all these approaches require electric supply and are usually characterized by a noticeable visual impact on the exhibition space, which clearly negatively affects the visitors’ experience. This paper intends to revisit and discuss a methodology (and its test application on field, to estimate the risk of damage of three artefacts displayed in an old museum of Palermo – Italy) perhaps prematurely underestimated that could usefully be re-proposed in particular contexts in which it is necessary to be careful to be as less invasive as possible, as in the case of museums housed in buildings of particular historical-cultural prestige. The discussed approach uses a non-destructive (or minimally invasive, at least) technique consisting in using two types of coupons, bi-metallic and marble ones, that allows an off-line monitoring of the indoor air aggressiveness inside museums, while avoiding consuming electricity and undesirable visual impacts on the site. Specifically, the estimated coupon damage is regarded as gauge to air aggressiveness, in order to provide an early warning of the risk of damage for the artefacts made of materials similar to those which the coupons are made of. Based on the considerations reported in the paper, the indirect evaluation of the air chemical aggressiveness through the assessment of the bi-metallic and marble coupons’ erosion seems to still candidates itself as a feasible method to be applied in complex exhibition scenes.
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- 2021
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7. Comparison Between Conventional and Vegetated Roof by Means of a Dynamic Simulation
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Ferrante, Patrizia, La Gennusa, Maria, Peri, Giorgia, Scaccianoce, Gianluca, and Sorrentino, Giancarlo
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- 2015
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8. Xanthomonas arboricola pv. fragariae: a confirmation of the pathogenicity of the pathotype strain
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Ferrante, Patrizia and Scortichini, Marco
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- 2017
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9. Toward a holistic environmental impact assessment of marble quarrying and processing: proposal of a novel easy-to-use IPAT-based method
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Capitano, Cinzia, Peri, Giorgia, Rizzo, Gianfranco, and Ferrante, Patrizia
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- 2017
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10. High-Dose Chemotherapy for Breast Cancer in Europe : From Fashion to Randomization
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Rosti, Giovanni, Ferrante, Patrizia, Marangolo, Maurizio, Crown, John, Roche, Henry, Viens, Patrice, Gratwohl, Alois, Abraham, Nader G., editor, Tabilio, Antonio, editor, Martelli, Massimo, editor, Asano, Shigetaka, editor, and Donfrancesco, Alberto, editor
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- 1999
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11. Hypoxic response in cancer progression: modeling HIF activation
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Ferrante, Patrizia, Toral, Raúl, and Flandoli, Franco
- Abstract
Trabajo Fin de Máster., The reprogramming of energy metabolism represents an important hallmark of cancer. The main cause of this alteration is represented by the presence of hypoxic areas. The decreased availability of oxygen activates the Hypoxia Inducible Factor (HIF) pathway which regulates the switch from an aerobic to an anaerobic metabolism, as well as an increased uptake of glucose. This thesis has two main objectives. In the first part we study and we nu- merically simulate a mathematical model consisting of four ordinary differential equations (ODEs): one for cancer cells with inactivated HIF signaling, one for cancer cells with activated HIF signaling and two for the metabolites cancer cells can consume in order to produce ATP. We model the dynamics of the transi- tion between the two different metabolic behaviors, of cancer growth under normoxic and hypoxic conditions and of cancer progression in a hostile environ- ment; furthermore we compare the behaviour of cancer cells in highlanders and lowlanders. In the second part we introduce a simple mathematical model to analyse the link between angiogenesis and the Warburg effect in cancer cells. We show that the increased expression of glucose receptors is an important factor in the transition from initiation to cancer progression. We also show that the genetic polymorphism considered in highlanders does not provide useful in- formation on cancer growth in the long term behaviour. The second model, despite only being introduced, will let us formulate new questions concerning the role of angiogenesis in cancer progression and links among different aspect of cancer metabolism.
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- 2020
12. Prediction of Strawberries' Quality Parameters Using Artificial Neural Networks.
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Amoriello, Tiziana, Ciccoritti, Roberto, and Ferrante, Patrizia
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ARTIFICIAL neural networks ,STRAWBERRIES ,1-Methylcyclopropene ,FRUIT industry ,HARVESTING time ,REGRESSION analysis ,FRUIT quality ,ANTHOCYANINS - Abstract
Strawberry is a very popular fruit, appreciated for its unique flavor and many beneficial traits such as antioxidants and useful amino acids, which strongly contribute to the overall quality of the product. Indeed, the quality of fresh fruit is a fundamental aspect for consumers, and it is crucial for the success of breeding activities as well as for enhancing the competitiveness and profitability of the fruit industry. Nowadays, the entire supply chain requires simple and fast systems for quality evaluation. In this context, the pomological and chemical traits (i.e., soluble solids, firmness, titratable acidity, dry matter) as well as nutritional ones such as total phenols, total anthocyanins and antioxidant potential were evaluated and compared for seven strawberry cultivars and three harvest times. The prediction of the qualitative traits was carried out using color space coordinates (L*, a* and b*) and two statistical techniques, i.e., the multiple linear regression models (MLR) and artificial neural networks (ANNs). Unsatisfactory prediction performances were obtained for all parameters when MLR was applied. On the contrary, the good prediction of the internal quality attributes, using ANN, was observed, especially for both antioxidant activity and the total monomeric anthocyanin (R
2 = 0.906, and R2 = 0.943, respectively). This study highlighted that color coordinates coupled with ANN can be successfully used to evaluate the quality of strawberry. [ABSTRACT FROM AUTHOR]- Published
- 2022
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13. Salvage high-dose chemotherapy in patients with germ cell tumor
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Rosti, Giovanni, De Giorgi, Ugo, Salvioni, Roberto, Papiani, Giorgio, Sebastiani, Loretta, Argnani, Marzia, Monti, Giuseppe, Ferrante, Patrizia, Pizzocaro, Giorgio, and Marangolo, Maurizio
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- 2002
14. The role of the transportation phase for a green management of municipal solid waste
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Ferrante, Patrizia, La Gennusa, Maria, Peri, Giorgia, Pianello, Claudia, Rizzo, Gianfranco, Ferrante, Patrizia, La Gennusa, Maria, Peri, Giorgia, Pianello, Claudia, and Rizzo, Gianfranco
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Municipal solid waste, environmental sustainability, transportation vehicles, ecological footprint, landfill - Abstract
Municipal solid waste management (MSWM) constitutes a high challenging issue to cope with to move towards sustainability of the urban policies. Despite new standards call for recycling and reusing materials contained in the city waste, several municipalities still use extensive landfills. Other than the environmental pressure exerted by these plants, the waste transportation from the recovery points of towns to landfills needs consideration to correctly assess the whole burden of the waste management. In this paper, the Ecological Footprint indicator is applied to the MSWM of the town of Palermo (Sicily). Results show that the effects produced by the involved transportation vehicles, both in the status-quo and in the hypothesized enhanced scenario (characterized by a reduced amount of urban waste landfilled), are relevant compared to those caused by the other segments of the waste management system. The concept of “saved footprint” is further introduced, to suitably compare the two scenarios.
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- 2017
15. Case Report: B Lymphocyte Disorders Under COVID-19 Inflammatory Pressure.
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Taliani, Gloria, Follini, Elena, Guglielmetti, Lorenzo, Bernuzzi, Patrizia, Faggi, Alberto, Ferrante, Patrizia, Fronti, Elisa, Gerna, Laura, Leoni, Maria Cristina, Paolillo, Franco, Ratti, Giovanna, Ruggieri, Alessandro, Valdatta, Caterina, Donisi, Alessandra, Zangrandi, Adriano, Pochintesta, Lara, Moroni, Carlo, Sacchini, Daria, Vallisa, Daniele, and Codeluppi, Mauro
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SARS-CoV-2 ,B cells ,COVID-19 ,AUTOIMMUNE hemolytic anemia - Abstract
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infects humans through the angiotensin converting enzyme-2 (ACE-2) receptor expressed on many cells, including lymphocytes. In Covid-19 patients IL-6 is overexpressed, and hyperactivated plasmacytoid lymphocytes are detected in peripheral blood film. We hypothesize that, due to the unpredictable interaction between the new virus and the B cell lineage of infected patients, a cascade of out of control events can ensue, capable of determining unexpected pathologic disorders involving such lineage. Here we report two cases of autoimmune hemolytic anemia (AIHA) and two cases of B-cell hematological malignancies developed or reactivated during acute SARS-CoV-2 infection. The temporal relationship of the events may suggest a potential causal relationship between SARS-CoV-2 infection and the hematopoietic disorders. We suggest that special attention should be paid to COVID-19 patients with underlining B cell lineage disorders. [ABSTRACT FROM AUTHOR]
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- 2021
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16. Phylogenetic, genetic, and phenotypic diversity of Pseudomonas syringae pv. syringae strains isolated from citrus blast and black pit in Tunisia.
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Abdellatif, Emna, Kałużna, Monika, Ferrante, Patrizia, Scortichini, Marco, Bahri, Bochra, Janse, Jaap D., Vaerenberg, Johan, Baeyen, Steve, Sobiczewski, Piotr, and Rhouma, Ali
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PSEUDOMONAS syringae ,CITRUS ,COPPER sulfate ,MOLECULAR phylogeny - Abstract
Citrus blast and black pit caused by Pseudomonas syringae pv. syringae (Pss) is the only bacterial disease reported in Tunisian Citrus orchards. The phylogenetic relationship between Pss strains was studied based on multilocus sequence analysis (MLSA), using partial sequences of housekeeping genes rpoD, rpoB, gyrB, cts, and pfk for 14 representative Pss Citrus strains, including the reference strain LMG5496. The MLSA revealed that the studied Tunisian Citrus strains are closely related to LMG5496 and cluster in phylogroup 02. Based on the cts gene, the majority of Citrus strains clustered in clades "a" and "b". However, five strains were placed in a newly defined clade "g". We describe the presence of six different type III secreted effectors (T3SEs). These were found with frequencies of 100% for the effector hopAN1 and the helper hrpK1, 65% for hopT1‐2, and 14% for hopN1, hopR1, and hopQ1‐2. Investigation of copper resistance showed that 67% of our Pss Citrus strains from Tunisia are resistant to copper sulphate in vitro, and the copper resistance genes copABCDR were detected in 23% of the strains. Our results present new data concerning the genetic diversity and phylogeny, presence of T3SEs, and copper resistance within the Pss populations that affect Citrus in Tunisia. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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17. Clostridium bifermentans and C. subterminale are associated with kiwifruit vine decline, known as moria, in Italy.
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Spigaglia, Patrizia, Barbanti, Fabrizio, Marocchi, Fabio, Mastroleo, Marco, Baretta, Marco, Ferrante, Patrizia, Caboni, Emilia, Lucioli, Simona, and Scortichini, Marco
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KIWIFRUIT ,WATERLOGGING (Soils) ,CLOSTRIDIUM ,ANAEROBIC bacteria ,IRRIGATION ,CLIMBING plants - Abstract
Since 2012, a new pathogenic syndrome has frequently been observed in many areas of kiwifruit cultivation in Italy. The main symptoms include an initial withering of the leaves followed by a total and sudden collapse of plants, mainly occurring during summer. The withered leaves fall and the main and secondary feeder roots appear rotten, sometimes showing a reddish‐brown discoloration. The disease, that affects both the green and yellow‐fleshed cultivars, has been called kiwifruit vine decline and is locally known as moria. The syndrome has been found consistently associated with soil waterlogging, which frequently occurs either after the traditional agronomical practice of irrigating orchards through surface irrigation or after very heavy rainfall. So far, the role played by bacteria in this syndrome has not been investigated. In the present study, Clostridium spp. were isolated from both rotten roots and soils obtained from Italian kiwifruit orchards affected by the syndrome, indicating for the first time that anaerobic bacteria are able to cause damage to woody crops. C. bifermentans and C. subterminale incited symptoms in kiwifruit in both in vivo and in vitro pathogenicity tests. Soil waterlogging seems to potentially favour colonization of kiwifruit roots by anaerobic bacteria, probably because saturation of the soil can facilitate proliferation and persistence of these bacteria during long periods of the vegetative growth of the crop. The occurrence of anaerobic bacteria does not exclude the possibility that other microorganisms can play additional/synergic role(s) in causing the kiwifruit vine decline. [ABSTRACT FROM AUTHOR]
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- 2020
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18. Transcriptome reprogramming of resistant and susceptible peach genotypes during Xanthomonas arboricola pv. pruni early leaf infection.
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Gervasi, Fabio, Ferrante, Patrizia, Dettori, Maria Teresa, Scortichini, Marco, and Verde, Ignazio
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XANTHOMONAS , *LEAF diseases & pests , *GENOTYPES , *RNA sequencing , *NUCLEOTIDE sequence - Abstract
Bacterial spot caused by Xanthomonas arboricola pv. pruni (Xap) is a major threat to Prunus species worldwide. The molecular mechanisms of peach resistance to Xap during early leaf infection were investigated by RNA-Seq analysis of two Prunus persica cultivars, ‘Redkist’ (resistant), and ‘JH Hale’ (susceptible) at 30 minutes, 1 and 3 hours-post-infection (hpi). Both cultivars exhibited extensive modulation of gene expression at 30 mpi, which reduced significantly at 1 hpi, increasing again at 3 hpi. Overall, 714 differentially expressed genes (DEGs) were detected in ‘Redkist’ (12% at 30 mpi and 1 hpi and 88% at 3 hpi). In ‘JH Hale’, 821 DEGs were identified (47% at 30 mpi and 1 hpi and 53% at 3 hpi). Highly up-regulated genes (fold change > 100) at 3 hpi exhibited higher fold change values in ‘Redkist’ than in ‘JH Hale’. RNA-Seq bioinformatics analyses were validated by RT-qPCR. In both cultivars, DEGs included genes with putative roles in perception, signal transduction, secondary metabolism, and transcription regulation, and there were defense responses in both cultivars, with enrichment for the gene ontology terms, ‘immune system process’, ‘defense response’, and ‘cell death’. There were particular differences between the cultivars in the intensity and kinetics of modulation of expression of genes with putative roles in transcriptional activity, secondary metabolism, photosynthesis, and receptor and signaling processes. Analysis of differential exon usage (DEU) revealed that both cultivars initiated remodeling their transcriptomes at 30 mpi; however, ‘Redkist’ exhibited alternative exon usage for a greater number of genes at every time point compared with ‘JH Hale’. Candidate resistance genes (WRKY-like, CRK-like, Copper amine oxidase-like, and TIR-NBS-LRR-like) are of interest for further functional characterization with the aim of elucidating their role in Prunus spp. resistance to Xap. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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19. Genomic Structural Variations Affecting Virulence During Clonal Expansion of Pseudomonas syringae pv. actinidiae Biovar 3 in Europe.
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Firrao, Giuseppe, Torelli, Emanuela, Polano, Cesare, Ferrante, Patrizia, Ferrini, Francesca, Martini, Marta, Marcelletti, Simone, Scortichini, Marco, and Ermacora, Paolo
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PSEUDOMONAS syringae ,VIRULENCE of bacteria ,BACTERIAL genomes - Abstract
Pseudomonas syringae pv. actinidiae (Psa) biovar 3 caused pandemic bacterial canker of Actinidia chinensis and Actinidia deliciosa since 2008. In Europe, the disease spread rapidly in the kiwifruit cultivation areas from a single introduction. In this study, we investigated the genomic diversity of Psa biovar 3 strains during the primary clonal expansion in Europe using single molecule real-time (SMRT), Illumina and Sanger sequencing technologies. We recorded evidences of frequent mobilization and loss of transposon Tn6212, large chromosome inversions, and ectopic integration of IS sequences (remarkably ISPsy31, ISPsy36, and ISPsy37). While no phenotype change associated with Tn6212 mobilization could be detected, strains CRAFRU 12.29 and CRAFRU 12.50 did not elicit the hypersensitivity response (HR) on tobacco and eggplant leaves and were limited in their growth in kiwifruit leaves due to insertion of ISPsy31 and ISPsy36 in the hrpS and hrpR genes, respectively, interrupting the hrp cluster. Both strains had been isolated from symptomatic plants, suggesting coexistence of variant strains with reduced virulence together with virulent strains in mixed populations. The structural differences caused by rearrangements of self-genetic elements within European and New Zealand strains were comparable in number and type to those occurring among the European strains, in contrast with the significant difference in terms of nucleotide polymorphisms. We hypothesize a relaxation, during clonal expansion, of the selection limiting the accumulation of deleterious mutations associated with genome structural variation due to transposition of mobile elements. This consideration may be relevant when evaluating strategies to be adopted for epidemics management. [ABSTRACT FROM AUTHOR]
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- 2018
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20. Xanthomonas arboricola pv. fragariae: a confirmation of the pathogenicity of the pathotype strain.
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Ferrante, Patrizia and Scortichini, Marco
- Abstract
Xanthomonas arboricola pv. fragariae is the causal agent of strawberry bacterial leaf blight, and, so far, has been reported in Italy and Turkey. Some years later the description of this new pathovar, and its pathogenicity, was questioned based upon artificial inoculations performed with some strains of the pathogen, including the pathotype strain; it was concluded that it is a non-pathogenic xanthomonad. This notion has also been accepted in other studies aiming at elucidating the molecular characteristics of X. arboricola strains. In the present investigation, we performed again pathogenicity tests using the pathotype strain of X. a. pv. fragariae, namely PD 2780 = LMG 19145 = CFBP 6771, by a spray leaf inoculation technique on strawberry cultivars Candonga, Sabrina and Murano. Similarly, it was also inoculated onto pot-cultivated walnut, hazelnut, peach, apple and kiwifruit plants. All typical symptoms of bacterial leaf blight of strawberry were observed within 25 days after the artificial inoculation on all tested strawberry cultivars. None of the other plant species showed symptoms of disease. We conclude again that the pathotype strain of X. a. pv. fragariae is a pathogenic bacterium. This technique also allowed us to observe typical symptoms of strawberry bacterial blight in a shorter time than that obtained previously by puncturing the major vein of the three leaflets of the leaf. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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21. Pseudomonas syringae pv. actinidiae: a re‐emerging, multi‐faceted, pandemic pathogen
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SCORTICHINI, MARCO, MARCELLETTI, SIMONE, FERRANTE, PATRIZIA, PETRICCIONE, MILENA, and FIRRAO, GIUSEPPE
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Virulence ,fungi ,food and beverages ,Pseudomonas syringae ,Pathogen Profile ,Biological Evolution ,Plant Diseases - Abstract
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker of green‐fleshed kiwifruit (Actinidia deliciosa) and yellow‐fleshed kiwifruit (A. chinensis). A recent, sudden, re‐emerging wave of this disease has occurred, almost contemporaneously, in all of the main areas of kiwifruit production in the world, suggesting that it can be considered as a pandemic disease. Recent in‐depth genetic studies performed on P. syringae pv. actinidiae strains have revealed that this pathovar is composed of four genetically different populations which, to different extents, can infect crops of the genus Actinidia worldwide. Genome comparisons of these strains have revealed that this pathovar can gain and lose the phaseolotoxin gene cluster, as well as mobile genetic elements, such as plasmids and putative prophages, and that it can modify the repertoire of the effector gene arrays. In addition, the strains currently causing worldwide severe economic losses display an extensive set of genes related to the ecological fitness of the bacterium in planta, such as copper and antibiotic resistance genes, multiple siderophore genes and genes involved in the degradation of lignin derivatives and other phenolics. This pathogen can therefore easily colonize hosts throughout the year. Taxonomy: Bacteria; Proteobacteria, gamma subdivision; Order Pseudomonadales; Family Pseudomonadaceae; Genus Pseudomonas; Pseudomonas syringae species complex, genomospecies 8; Pathovar actinidiae. Microbiological properties: Gram‐negative, aerobic, motile, rod‐shaped, polar flagella, oxidase‐negative, arginine dihydrolase‐negative, DNA 58.5–58.8 mol.% GC, elicits the hypersensitive response on tobacco leaves. Host range: Primarily studied as the causal agent of bacterial canker of green‐fleshed kiwifruit (Actinidia deliciosa), it has also been isolated from yellow‐fleshed kiwifruit (A. chinensis). In both species, it causes severe economic losses worldwide. It has also been isolated from wild A. arguta and A. kolomikta. Disease symptoms: In green‐fleshed and yellow‐fleshed kiwifruits, the symptoms include brown–black leaf spots often surrounded by a chlorotic margin, blossom necrosis, extensive twig die‐back, reddening of the lenticels, extensive cankers along the main trunk and leader, and bleeding cankers on the trunk and the leader with a whitish to orange ooze. Epidemiology: Pseudomonas syringae pv. actinidiae can effectively colonize its host plants throughout the year. Bacterial exudates can disperse a large amount of inoculum within and between orchards. In the spring, temperatures ranging from 12 to 18 °C, together with humid conditions, can greatly favour the multiplication of the bacterium, allowing it to systemically move from the leaf to the young shoots. During the summer, very high temperatures can reduce the multiplication and dispersal of the bacterium. Some agronomical techniques, as well as frost, wind, rain and hail storms, can contribute to further spreading. Disease control: An integrated approach that takes into consideration precise scheduled spray treatments with effective and environmentally friendly bactericides and equilibrated plant nutrition, coupled with preventive measures aimed at drastically reducing the bacterial inoculum, currently seems to be the possible best solution for coexistence with the disease. The development of resistant cultivars and pollinators, effective biocontrol agents, including bacteriophages, and compounds that induce the systemic activation of plant defence mechanisms is in progress. Useful websites: Up‐to‐date information on bacterial canker research progress and on the spread of the disease in New Zealand can be found at: http://www.kvh.org.nz. Daily information on the spread of the disease and on the research being performed worldwide can be found at: http://www.freshplaza.it.
- Published
- 2012
22. Off-line methods for determining air quality in museums
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Franzitta, Vincenzo, Ferrante, Patrizia, La Gennusa, Maria, Rizzo, Gianfranco, Scaccianoce, Gianluca, Franzitta, V, Ferrante, P, La Gennusa, M, Rizzo, G, and Scaccianoce, G
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air quality ,monitoring air quality ,off-line sensors of air quality ,air conditioned museum environments ,Settore ING-IND/11 - Fisica Tecnica Ambientale ,lcsh:NX1-820 ,air quality, monitoring air quality, off-line sensors, air-conditioned museum environments ,lcsh:Arts in general - Abstract
This paper will examine three methodologies deployed in off-line research relating to air quality in museums. These methodologies made use of sensors based on the different chemical-physicalproperties of various materials (corrosiveness, hardness and absorption). Various field applications were applied to case studies, which were performed in two museums in Palermo, Italy: the Regional Gallery of Palazzo Abatellis and the Diocesan Museum. This review of methodologies and sensors examines which protocol should be put into practice in museums in monitoring the air quality of display spaces and the conservation of cultural artefacts.
- Published
- 2010
23. The use of energy in the Mediterranean fishing catching sector. A Sicilian perspective
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LA GENNUSA, Maria, FERRANTE, Patrizia, PERNICE, Riccardo, Barone,A, La Gennusa,M, Ferrante,P, Barone,A, and Pernice,R
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catching fishing sector, energy efficiency, sustainability, indicators ,Settore ING-IND/11 - Fisica Tecnica Ambientale - Abstract
This article address an issue of paramount importance in the Mediterranean area, that is the efficient use of energy and sustainability of fishing sector. A recent study concerning the situation of Fishery and Aquaculture in Sicily highlighted that the Sicilian fishing system requires a bigger amount of energy than the typical Italian or European situation, in order of getting the product available to the market. On the other hand this involves an higher level of environmental pollution, for a given quantity of catch. One of the main findings is that there is a need for a clear, scientifically-founded panorama of the situation and that further studies should be undertaken to provide fishing operators with adequate guidance in order to choose fishing practises and energy technologies that are more efficient in terms of energy expenditure by unit of revenue from catch. At this aim, this work carries out a concise analysis of catching segment of the fishing sector in Europe, in Italy and Sicily, starting from literature data. Some productivity, economic, capacity and efficiency indicators will be analyze and suggest in order to make a comparative comparison among the three geographic ambits.
- Published
- 2010
24. Characterization of Pseudomonas savastanoi pv. savastanoi strains collected from olive trees in different Countries
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Moretti, Chiaraluce, Ferrante, Patrizia, Hosni, T., D'Onghia, A. M., Fatmi, M., and Buonaurio, Roberto
- Published
- 2008
25. Comparison of methodologies for Test Reference Year (TRY) generation for Mediterranean sites
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FERRANTE, Patrizia, LA GENNUSA, Maria, SCACCIANOCE, Gianluca, SORRENTINO, Giancarlo, NICOLOSI S, FERRANTE P, LA GENNUSA M, NICOLOSI S, SCACCIANOCE G, and SORRENTINO G
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TRY, solar radiation, climatic data ,Settore ING-IND/11 - Fisica Tecnica Ambientale - Abstract
The improvement of the energy efficiency of buildings calls for the availability of tools for evaluating and simulating the thermal behaviour of buildings. These simulation tools generally need a complete input data set and, in particular, information referring to the climatic conditions of the site where buildings are built-up. Among climatic issues, particularly important for the thermal energy balance is the solar radiation. However, this huge mass of data is not very useful in this form, so simple aggregation methods have been developed in order to run these simulation computer programs: the test reference year (TRY) is one of the most used methods. In this paper the comparison of two methodologies for compiling TRY of the solar radiation is presented. The two methodologies, elaborated respectively by Dogniaux and Finkelstein-Schafer, have both a statistical approach to the problem. The first one is based on the normal distribution of the monthly average of each climatic parameter and his variance, while the second one is a more powerful version of the Kolmogorov-Smirnov test, based on the cumulative probability function. The two methods give quite different results, suggesting a particular caution in the choice of the method for generating such reduced sets of data.
- Published
- 2008
26. Phylogenetic characterization of Pseudomonas syringae pv. tabaci and the role played by the effector protein hopQ1-1 in determining its virulence and host specificity
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Ferrante, Patrizia, Buonaurio, Roberto, and Vinatzer, B. A.
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- 2007
27. Evaluation of some chemicals as inducers of resistance in tomato plants against Pseudomonas syringae pv. tomato
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Silvestri, Simona, Moretti, Chiaraluce, Ferrante, Patrizia, and Buonaurio, Roberto
- Published
- 2007
28. Characterization of Pseudomonas syringae pv. tabaci strains collected in Central Italy
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Ferrante, Patrizia, Moretti, Chiaraluce, and Buonaurio, Roberto
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tabtoxin ,Pseudomonas syringae pv. tabaci ,Nicotiana tabacum - Published
- 2006
29. Identification of Loci of Pseudomonas syringae pv. actinidiae Involved in Lipolytic Activity and Their Role in Colonization of Kiwifruit Leaves.
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Patel, Hitendra Kumar, Ferrante, Patrizia, Meng Xianfa, Javvadi, Sree Gowrinadh, Subramoni, Sujatha, Scortichini, Marco, and Venturi, Vittorio
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PSEUDOMONAS syringae , *KIWIFRUIT , *THERAPEUTICS ,PSEUDOMONAS physiology - Abstract
Bacterial canker disease caused by Pseudomonas syringae pv. actinidiae, an emerging pathogen of kiwifruit plants, has recently brought about major economic losses worldwide. Genetic studies on virulence functions of P. syringae pv. actinidiae have not yet been reported and there is little experimental data regarding bacterial genes involved in pathogenesis. In this study, we performed a genetic screen in order to identify transposon mutants altered in the lipolytic activity because it is known that mechanisms of regulation, production, and secretion of enzymes often play crucial roles in virulence of plant pathogens. We aimed to identify the set of secretion and global regulatory loci that control lipolytic activity and also play important roles in in planta fitness. Our screen for altered lipolytic activity phenotype identified a total of 58 TnJ transposon mutants. Mapping all these Tn5 mutants revealed that the transposons were inserted in genes that play roles in cell division, chemotaxis, metabolism, movement, recombination, regulation, signal transduction, and transport as well as a few unknown functions. Several of these identified P. syringae pv. actinidiae Tn5 mutants, notably the functions affected in phosphomannomutase AlgC, lipid A biosynthesis acyltransfera.se, glutamate-cysteine ligase, and the type IV pilus protein Pill, were also found affected in in planta survival and/or growth in kiwifruit plants. The results of the genetic screen and identification of novel loci involved in in planta fitness of P. syringae pv. actinidiae are presented and discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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30. Composite Building Materials: Thermal and Mechanical Performances of Samples Realized with Hay and Natural Resins.
- Author
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La Gennusa, Maria, Llorach-Massana, Pere, Montero, Juan Ignacio, Peña, Francisco Javier, Rieradevall, Joan, Ferrante, Patrizia, Scaccianoce, Gianluca, and Sorrentino, Giancarlo
- Abstract
Recent years have seen an increasing public interest in issues related to energy saving and environmental pollution reduction in the building sector. As a result, many directives have been issued, the most important being the Directive 2010/31/EU (EPBD Recast) on the energy performance of buildings, which requires that "Member States shall ensure that by 31 December 2020 all new buildings are nearly zero-energy buildings". This goal can be obtained not only by reducing energy demand for heating and cooling, but also, for example, by improving building envelope performances. In this work, a first analysis of the thermal and structural behaviour of a biocomposite material, constituted by a natural resin (rosin) and vegetal fibres (hay), has been performed, with particular attention to the share of fibres and the granulometry in the mixture. The biocomposite has shown both good insulation properties and mechanical resistance. However, the results show that further analyses should be performed on the optimisation of the samples' preparation process. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
31. Genome Plasticity and Dynamic Evolution of Phytopathogenic Pseudomonads and Related Bacteria.
- Author
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Scortichini, Marco, Marcelletti, Simone, Ferrante, Patrizia, Petriccione, Milena, Torelli, Emanuela, and Firrao, Giuseppe
- Published
- 2014
- Full Text
- View/download PDF
32. An Integrated Environmental Indicator for Urban Transportation Systems: Description and Application.
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La Gennusa, Maria, Ferrante, Patrizia, Casto, Barbara Lo, and Rizzo, Gianfranco
- Subjects
- *
ENVIRONMENTAL indicators , *URBAN transportation , *ECOLOGICAL impact , *EMISSIONS (Air pollution) , *POLLUTANTS , *TRANSPORTATION & the environment - Abstract
A simplified version of the ecological footprint method is proposed for assessing the environmental performances of urban transportation systems. The method, starting from the knowledge of the composition of the running vehicular fleet, is here applied to a southern Italian province. It represents a synthetic indicator of the environmental pressure exerted by the system also matching the pollutant emissions with the carrying capacity of the site. Particularly, the forested area needed to absorb the CO2 emissions of the system is compared with the total forested area of the province. The results of the case-study indicates the yearly maximum distance that each vehicle of the fleet can cover in order for their emissions to be absorbed by the surrounding forested area. Specifically, if all cars of the fleet would travel for 10,000 km/year, 97% of the forested area would be involved. Thanks to its features, this indicator can be usefully adopted for ranking different transportation options. Therefore, it could allow local administrations to environmentally hierarchize alternative plans concerning urban transportation choices. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
33. Isolation and partial characterization of bacteriophages infecting Pseudomonas syringae pv. actinidiae, causal agent of kiwifruit bacterial canker.
- Author
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Di Lallo, Gustavo, Evangelisti, Matteo, Mancuso, Francesco, Ferrante, Patrizia, Marcelletti, Simone, Tinari, Antonella, Superti, Fabiana, Migliore, Luciana, D'Addabbo, Pietro, Frezza, Domenico, Scortichini, Marco, and Thaller, Maria Cristina
- Subjects
BACTERIOPHAGES ,PSEUDOMONAS syringae ,ACTINIDIA ,BACTERIAL diseases of plants ,KIWIFRUIT ,MICROBIOLOGY - Abstract
The phytopathogen Pseudomonas syringae pv. actinidiae ( Psa) is the causal agent of bacterial canker of kiwifruit. In the last years, it has caused severe economic losses to Actinidia spp. cultivations, mainly in Italy and New Zealand. Conventional strategies adopted did not provide adequate control of infection. Phage therapy may be a realistic and safe answer to the urgent need for novel antibacterial agents aiming to control this bacterial pathogen. In this study, we described the isolation and characterization of two bacteriophages able to specifically infect Psa. φPSA1, a member of the Siphoviridae family, is a temperate phage with a narrow host range, a long latency, and a burst size of 178; φPSA2 is a lytic phage of Podoviridae family with a broader host range, a short latency, a burst size of 92 and a higher bactericidal activity as determined by the TOD value. The genomic sequence of φPSA1 has a length of 51,090 bp and a low sequence homology with the other siphophages, whereas φPSA2 has a length of 40 472 bp with a 98% homology with Pseudomonas putida bacteriophage gh-1. Of the two phages examined, φPSA2 may be considered as a candidate for phage therapy of kiwifruit disease, while φPSA1 seems specific toward the recent outbreak's isolates and could be useful for Psa typing. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
34. The Kiwifruit Emerging Pathogen Pseudomonas syringae pv. actinidiae Does Not Produce AHLs but Possesses Three LuxR Solos.
- Author
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Patel, Hitendra Kumar, Ferrante, Patrizia, Covaceuszach, Sonia, Lamba, Doriano, Scortichini, Marco, and Venturi, Vittorio
- Subjects
- *
PHYTOPATHOGENIC microorganisms , *KIWIFRUIT , *PSEUDOMONAS syringae , *QUORUM sensing , *N-acyl-L-homoserine lactone , *MICROBIOLOGY , *PLANTS - Abstract
Pseudomonas syringae pv. actinidiae (Psa) is an emerging phytopathogen causing bacterial canker disease in kiwifruit plants worldwide. Quorum sensing (QS) gene regulation plays important roles in many different bacterial plant pathogens. In this study we analyzed the presence and possible role of N-acyl homoserine lactone (AHL) quorum sensing in Psa. It was established that Psa does not produce AHLs and that a typical complete LuxI/R QS system is absent in Psa strains. Psa however possesses three putative luxR solos designated here as PsaR1, PsaR2 and PsaR3. PsaR2 belongs to the sub-family of LuxR solos present in many plant associated bacteria (PAB) that binds and responds to yet unknown plant signal molecules. PsaR1 and PsaR3 are highly similar to LuxRs which bind AHLs and are part of the canonical LuxI/R AHL QS systems. Mutation in all the three luxR solos of Psa showed reduction of in planta survival and also showed additive effect if more than one solo was inactivated in double mutants. Gene promoter analysis revealed that the three solos are not auto-regulated and investigated their possible role in several bacterial phenotypes. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
35. Pseudomonas syringae pv. actinidiae Draft Genomes Comparison Reveal Strain-Specific Features Involved in Adaptation and Virulence to Actinidia Species.
- Author
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Marcelletti, Simone, Ferrante, Patrizia, Petriccione, Milena, Firrao, Giuseppe, and Scortichini, Marco
- Subjects
- *
PSEUDOMONAS syringae , *GENOMES , *BIOLOGICAL adaptation , *MICROBIAL virulence , *ACTINIDIA - Abstract
A recent re-emerging bacterial canker disease incited by Pseudomonas syringae pv. actinidiae (Psa) is causing severe economic losses to Actinidia chinensis and A. deliciosa cultivations in southern Europe, New Zealand, Chile and South Korea. Little is known about the genetic features of this pathovar. We generated genome-wide Illumina sequence data from two Psa strains causing outbreaks of bacterial canker on the A. deliciosa cv. Hayward in Japan (J-Psa, type-strain of the pathovar) and in Italy (I-Psa) in 1984 and 1992, respectively as well as from a Psa strain (I2-Psa) isolated at the beginning of the recent epidemic on A. chinensis cv. Hort16A in Italy. All strains were isolated from typical leaf spot symptoms. The phylogenetic relationships revealed that Psa is more closely related to P. s. pv. theae than to P. avellanae within genomospecies 8. Comparative genomic analyses revealed both relevant intrapathovar variations and putative pathovar-specific genomic regions in Psa. The genomic sequences of J-Psa and I-Psa were very similar. Conversely, the I2-Psa genome encodes four additional effector protein genes, lacks a 50 kb plasmid and the phaseolotoxin gene cluster, argK-tox but has acquired a 160 kb plasmid and putative prophage sequences. Several lines of evidence from the analysis of the genome sequences support the hypothesis that this strain did not evolve from the Psa population that caused the epidemics in 1984-1992 in Japan and Italy but rather is the product of a recent independent evolution of the pathovar actinidiae for infecting Actinidia spp. All Psa strains share the genetic potential for copper resistance, antibiotic detoxification, high affinity iron acquisition and detoxification of nitric oxide of plant origin. Similar to other sequenced phytopathogenic pseudomonads associated with woody plant species, the Psa strains isolated from leaves also display a set of genes involved in the catabolism of plantderived aromatic compounds. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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- View/download PDF
36. Multilocus Sequence Typing Reveals Relevant Genetic Variation and Different Evolutionary Dynamics among Strains of Xanthomonas arboricola pv. juglandis.
- Author
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Marcelletti, Simone, Ferrante, Patrizia, and Scortichini, Marco
- Subjects
- *
PLANT molecular genetics , *PLANT genetics , *PLANT population genetics , *PLANT phylogeny , *PLANT populations , *PLANT evolution , *POPULATION dynamics , *BIOLOGICAL adaptation , *GENETIC recombination - Abstract
Forty-five Xanthomonas arboricola pv. juglandis (Xaj) strains originating from Juglans regia cultivation in different countries were molecularly typed by means of MultiLocus Sequence Typing (MLST), using acnB, gapA, gyrB and rpoD gene fragments. A total of 2.5 kilobases was used to infer the phylogenetic relationship among the strains and possible recombination events. Haplotype diversity, linkage disequilibrium analysis, selection tests, gene flow estimates and codon adaptation index were also assessed. The dendrograms built by maximum likelihood with concatenated nucleotide and amino acid sequences revealed two major and two minor phylotypes. The same haplotype was found in strains originating from different continents, and different haplotypes were found in strains isolated in the same year from the same location. A recombination breakpoint was detected within the rpoD gene fragment. At the pathovar level, the Xaj populations studied here are clonal and under neutral selection. However, four Xaj strains isolated from walnut fruits with apical necrosis are under diversifying selection, suggesting a possible new adaptation. Gene flow estimates do not support the hypothesis of geographic isolation of the strains, even though the genetic diversity between the strains increases as the geographic distance between them increases. A triplet deletion, causing the absence of valine, was found in the rpoD fragment of all 45 Xaj strains when compared with X. axonopodis pv. citri strain 306. The codon adaptation index was high in all four genes studied, indicating a relevant metabolic activity. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
37. Contributions of the effector gene hopQ1-1 to differences in host range between Pseudomonas syringae pv. phaseolicola and P. syringae pv. tabaci.
- Author
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Ferrante, Patrizia, Clarke, Christopher R., Cavanaugh, Keri A., Michelmore, Richard W., Buonaurio, Roberto, and Vinatzer, Boris A.
- Subjects
- *
PSEUDOMONAS syringae , *INSECT host plants , *BEAN genetics , *PLANT disease research , *PHYLOGENY , *GENOMES , *PLANT immunology , *MICROBIAL virulence - Abstract
To study the role of type III-secreted effectors in the host adaptation of the tobacco ( Nicotiana sp.) pathogen Pseudomonas syringae pv. tabaci, a selection of seven strains was first characterized by multilocus sequence typing (MLST) to determine their phylogenetic affinity. MLST revealed that all strains represented a tight phylogenetic group and that the most closely related strain with a completely sequenced genome was the bean ( Phaseolus vulgaris) pathogen P. syringae pv. phaseolicola 1448A. Using primers designed to 21 P. syringae pv. phaseolicola 1448A effector genes, it was determined that P. syringae pv. phaseolicola 1448A shared at least 10 effectors with all tested P. syringae pv. tabaci strains. Six of the 11 effectors that failed to amplify from P. syringae pv. tabaci strains were individually expressed in one P. syringae pv. tabaci strain. Although five effectors had no effect on phenotype, growth in planta and disease severity of the transgenic P. syringae pv. tabaci expressing hopQ1-1Pph1448A were significantly increased in bean, but reduced in tobacco. We conclude that hopQ1-1 has been retained in P. syringae pv. phaseolicola 1448A, as this effector suppresses immunity in bean, whereas hopQ1-1 is missing from P. syringae pv. tabaci strains because it triggers defences in Nicotiana spp. This provides evidence that fine-tuning effector repertoires during host adaptation lead to a concomitant reduction in virulence in non-host species. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
38. <atl>High-dose chemotherapy for solid tumors: results of the EBMT
- Author
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Rosti, Giovanni, Ferrante, Patrizia, Ledermann, Jonathan, Leyvraz, Serge, Ladenstein, Ruth, Koscileniak, Ewa, Crown, John, Dazzi, Claudio, Cariello, Anna, and Marangolo, Maurizio
- Subjects
- *
TUMOR treatment , *BONE marrow transplantation - Abstract
The European Group for Blood and Marrow Transplantation (EBMT), formerly known as European Group for Bone Marrow Transplantation, was established in 1974 in the Netherlands to share experiences, to promote research and clinical studies and to set up registries in the field of hematopoietic tissue transplantation. At the present time more 400 European and non-European centers are members of the EBMT group. In 1984 a new Working Party was created (Solid Tumors) with the aim to investigate the role of high-dose chemotherapy and stem cell support in the fields of adult and pediatric solid tumors. By January 2000 more than 14 000 patients were registered, and at the present time this Registry is the world largest database on this subject. Several phase III randomized clinical trials have recently started on behalf of the Group in different diseases (breast carcinoma, small cell lung cancer, ovarian carcinoma, germ cell tumors and Ewing''s family sarcoma). Hundreds of randomized patients will finally produce clearer information on this still experimental therapeutic modality. This paper will describe the EBMT Solid Tumors Working Party Registry updated results as well as the main ongoing studies. [Copyright &y& Elsevier]
- Published
- 2002
- Full Text
- View/download PDF
39. High-Dose Chemotherapy and Autologous Stem Cell Transplantation in Patients with Breast Cancer and Other Solid Turnors. A Review of The EBMT Data.
- Author
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ROSTI, Giovanni, FERRANTE, Patrizia, CROWN, John, PICO, Jose Luis, LEYVRAZ, Serge, LEDERMANN, Jonathan, DEMİRER, Taner, CARIELLO, Anna, and GIOVANIS, Petros
- Published
- 1999
40. Omics, epidemiology and integrated approach for the coexistence with bacterial canker of kiwifruit, caused by Pseudomonas syringae pv. actinidiae.
- Author
-
Scortichini, Marco, Ferrante, Patrizia, Marcelletti, Simone, and Petriccione, Milena
- Subjects
KIWIFRUIT ,PLANT epidemiology ,BACTERIAL diseases of plants ,PSEUDOMONAS syringae ,MOLECULAR microbiology - Abstract
Bacterial canker of kiwifruit, caused by Pseudomonas syringae pv. actinidiae, is a destructive disease found in all major areas of production of green-fleshed (Actinidia deliciosa) and yellow-fleshed (A. chinensis) kiwifruit of the world (i.e, Europe, China, New Zealand and Chile). A series of studies and field trials concerning epidemiology, agronomical techniques, new bactericides effectiveness as well as molecular typing analysis, genomic and proteomic, allowed us to elucidate the cycle of disease of the pathogen, to dissect its main genomic features, to point out the plant proteins involved in resistance/tolerance to the bacterium, to modify some basic agronomical techniques and to propose new compounds that currently, at least in the province of Latina and Rome, Italy, allow the farmers to coexist with the pathogen by reaching the full yield and quality of the crop as before the appearance of the disease. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
41. Identification of Pseudomonas syringae pv . actinidiae as Causal Agent of Bacterial Canker of Yellow Kiwifruit ( Actinidia chinensis Planchon) in Central Italy.
- Author
-
Ferrante, Patrizia and Scortichini, Marco
- Subjects
- *
PSEUDOMONAS syringae , *KIWIFRUIT , *LEAF spots , *ORCHARDS - Abstract
Angular, necrotic leaf spot, longitudinal cracks along the petiole, oozing and wilting of branches were observed during summer 2008 on Actinidia chinensis (yellow kiwifruit) cultivar Hort16A, cultivated in different orchards located the province of Latina (central Italy). Symptoms closely resembled those incited by Pseudomonas syringae pv. actinidiae on kiwifruit A. deliciosa. Isolates obtained from typical lesions were assessed by means of biochemical, pathogenicity and host range tests and compared with some Pseudomonas syringae pathovars by enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis. The isolates belong to Pseudomonas LOPAT group Ia, incited the death of pot-cultivated A. chinensis cv. Hort 16A and A. deliciosa cv. Hayward plants in few days, but did not cause any symptoms to the other inoculated plant species. Upon ERIC-PCR analysis, all the isolates showed similarity with P. syringae pv. actinidiae NCPPB 3739, type-strain of the pathovar. This is the first report of this pathogen on A. chinensis in Italy and, as far as we currently know, in the world. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
42. A Genomic Redefinition of Pseudomonas avellanae species.
- Author
-
Scortichini, Marco, Marcelletti, Simone, Ferrante, Patrizia, and Firrao, Giuseppe
- Subjects
PSEUDOMONAS ,BACTERIAL genomes ,DNA ,SPECIES ,NUCLEOTIDE sequence ,PHYLOGENY ,GENETIC code - Abstract
The circumscription of bacterial species is a complex task. So far, DNA-DNA hybridization (DDH), 16S rRNA gene sequencing, and multiocus sequence typing analysis (MLSA) are currently the preferred techniques for their genetic determination. However, the average nucleotide identity (ANI) analysis of conserved and shared genes between two bacterial strains based on the pair-wise genome comparisons, with support of the tetranucleotide frequency correlation coefficients (TETRA) value, has recently been proposed as a reliable substitute for DDH. The species demarcation boundary has been set to a value of 95-96% of the ANI identity, with further confirmation through the assessment of the corresponding TETRA value. In this study, we performed a genome-wide MLSA of 14 phytopathogenic pseudomonads genomes, and assessed the ANI and TETRA values of 27 genomes, representing seven out of the nine genomospecies of Pseudomonas spp. sensu Gardan et alii, and their phylogenetic relationships using maximum likelihood and Bayesian approaches. The results demonstrate the existence of a well demarcated genomic cluster that includes strains classified as P. avellanae, P. syringae pv. theae, P. s. pv. actinidiae and one P. s. pv. morsprunorum strain all belonging to the single species P. avellanae. In addition, when compared with P. avellanae, five strains of P. s. pv. tomato, including the model strain DC3000, and one P. s. pv. lachrymans strain, appear as very closely related to P. avellanae, with ANI values of nearly 96% as confirmed by the TETRA analysis. Conversely, one representative strain, previously classified as P. avellanae and isolated in central Italy, is a genuine member of the P. syringae species complex and can be defined as P. s. pv. avellanae. Currently. The core and pan genomes of P. avellanae species consist of 3,995 and 5,410 putative protein-coding genes, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
43. The kiwifruit emerging pathogen Pseudomonas syringae pv. actinidiae does not produce AHLs but possesses three luxR solos.
- Author
-
Patel HK, Ferrante P, Covaceuszach S, Lamba D, Scortichini M, and Venturi V
- Subjects
- Pseudomonas syringae genetics, Repressor Proteins genetics, Trans-Activators genetics, Actinidia microbiology, Acyl-Butyrolactones, Plant Diseases microbiology, Pseudomonas syringae metabolism, Repressor Proteins metabolism, Trans-Activators metabolism
- Abstract
Pseudomonas syringae pv. actinidiae (Psa) is an emerging phytopathogen causing bacterial canker disease in kiwifruit plants worldwide. Quorum sensing (QS) gene regulation plays important roles in many different bacterial plant pathogens. In this study we analyzed the presence and possible role of N-acyl homoserine lactone (AHL) quorum sensing in Psa. It was established that Psa does not produce AHLs and that a typical complete LuxI/R QS system is absent in Psa strains. Psa however possesses three putative luxR solos designated here as PsaR1, PsaR2 and PsaR3. PsaR2 belongs to the sub-family of LuxR solos present in many plant associated bacteria (PAB) that binds and responds to yet unknown plant signal molecules. PsaR1 and PsaR3 are highly similar to LuxRs which bind AHLs and are part of the canonical LuxI/R AHL QS systems. Mutation in all the three luxR solos of Psa showed reduction of in planta survival and also showed additive effect if more than one solo was inactivated in double mutants. Gene promoter analysis revealed that the three solos are not auto-regulated and investigated their possible role in several bacterial phenotypes.
- Published
- 2014
- Full Text
- View/download PDF
44. A Genomic redefinition of Pseudomonas avellanae species.
- Author
-
Scortichini M, Marcelletti S, Ferrante P, and Firrao G
- Subjects
- Bayes Theorem, DNA, Bacterial genetics, Genomics methods, Nucleic Acid Hybridization genetics, Nucleotides genetics, Phylogeny, Sequence Analysis, DNA methods, Genome, Bacterial genetics, Pseudomonas classification, Pseudomonas genetics
- Abstract
The circumscription of bacterial species is a complex task. So far, DNA-DNA hybridization (DDH), 16S rRNA gene sequencing, and multiocus sequence typing analysis (MLSA) are currently the preferred techniques for their genetic determination. However, the average nucleotide identity (ANI) analysis of conserved and shared genes between two bacterial strains based on the pair-wise genome comparisons, with support of the tetranucleotide frequency correlation coefficients (TETRA) value, has recently been proposed as a reliable substitute for DDH. The species demarcation boundary has been set to a value of 95-96% of the ANI identity, with further confirmation through the assessment of the corresponding TETRA value. In this study, we performed a genome-wide MLSA of 14 phytopathogenic pseudomonads genomes, and assessed the ANI and TETRA values of 27 genomes, representing seven out of the nine genomospecies of Pseudomonas spp. sensu Gardan et alii, and their phylogenetic relationships using maximum likelihood and Bayesian approaches. The results demonstrate the existence of a well demarcated genomic cluster that includes strains classified as P. avellanae, P. syringae pv. theae, P. s. pv. actinidiae and one P. s. pv. morsprunorum strain all belonging to the single species P. avellanae. In addition, when compared with P. avellanae, five strains of P. s. pv. tomato, including the model strain DC3000, and one P. s. pv. lachrymans strain, appear as very closely related to P. avellanae, with ANI values of nearly 96% as confirmed by the TETRA analysis. Conversely, one representative strain, previously classified as P. avellanae and isolated in central Italy, is a genuine member of the P. syringae species complex and can be defined as P. s. pv. avellanae. Currently. The core and pan genomes of P. avellanae species consist of 3,995 and 5,410 putative protein-coding genes, respectively.
- Published
- 2013
- Full Text
- View/download PDF
45. Pseudomonas syringae pv. actinidiae: a re-emerging, multi-faceted, pandemic pathogen.
- Author
-
Scortichini M, Marcelletti S, Ferrante P, Petriccione M, and Firrao G
- Subjects
- Biological Evolution, Plant Diseases microbiology, Plant Diseases statistics & numerical data, Pseudomonas syringae classification, Pseudomonas syringae genetics, Pseudomonas syringae isolation & purification, Virulence, Pseudomonas syringae physiology
- Abstract
Pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker of green-fleshed kiwifruit (Actinidia deliciosa) and yellow-fleshed kiwifruit (A. chinensis). A recent, sudden, re-emerging wave of this disease has occurred, almost contemporaneously, in all of the main areas of kiwifruit production in the world, suggesting that it can be considered as a pandemic disease. Recent in-depth genetic studies performed on P. syringae pv. actinidiae strains have revealed that this pathovar is composed of four genetically different populations which, to different extents, can infect crops of the genus Actinidia worldwide. Genome comparisons of these strains have revealed that this pathovar can gain and lose the phaseolotoxin gene cluster, as well as mobile genetic elements, such as plasmids and putative prophages, and that it can modify the repertoire of the effector gene arrays. In addition, the strains currently causing worldwide severe economic losses display an extensive set of genes related to the ecological fitness of the bacterium in planta, such as copper and antibiotic resistance genes, multiple siderophore genes and genes involved in the degradation of lignin derivatives and other phenolics. This pathogen can therefore easily colonize hosts throughout the year., Taxonomy: Bacteria; Proteobacteria, gamma subdivision; Order Pseudomonadales; Family Pseudomonadaceae; Genus Pseudomonas; Pseudomonas syringae species complex, genomospecies 8; Pathovar actinidiae., Microbiological Properties: Gram-negative, aerobic, motile, rod-shaped, polar flagella, oxidase-negative, arginine dihydrolase-negative, DNA 58.5-58.8 mol.% GC, elicits the hypersensitive response on tobacco leaves., Host Range: Primarily studied as the causal agent of bacterial canker of green-fleshed kiwifruit (Actinidia deliciosa), it has also been isolated from yellow-fleshed kiwifruit (A. chinensis). In both species, it causes severe economic losses worldwide. It has also been isolated from wild A. arguta and A. kolomikta., Disease Symptoms: In green-fleshed and yellow-fleshed kiwifruits, the symptoms include brown-black leaf spots often surrounded by a chlorotic margin, blossom necrosis, extensive twig die-back, reddening of the lenticels, extensive cankers along the main trunk and leader, and bleeding cankers on the trunk and the leader with a whitish to orange ooze., Epidemiology: Pseudomonas syringae pv. actinidiae can effectively colonize its host plants throughout the year. Bacterial exudates can disperse a large amount of inoculum within and between orchards. In the spring, temperatures ranging from 12 to 18 °C, together with humid conditions, can greatly favour the multiplication of the bacterium, allowing it to systemically move from the leaf to the young shoots. During the summer, very high temperatures can reduce the multiplication and dispersal of the bacterium. Some agronomical techniques, as well as frost, wind, rain and hail storms, can contribute to further spreading., Disease Control: An integrated approach that takes into consideration precise scheduled spray treatments with effective and environmentally friendly bactericides and equilibrated plant nutrition, coupled with preventive measures aimed at drastically reducing the bacterial inoculum, currently seems to be the possible best solution for coexistence with the disease. The development of resistant cultivars and pollinators, effective biocontrol agents, including bacteriophages, and compounds that induce the systemic activation of plant defence mechanisms is in progress., Useful Websites: Up-to-date information on bacterial canker research progress and on the spread of the disease in New Zealand can be found at: http://www.kvh.org.nz. Daily information on the spread of the disease and on the research being performed worldwide can be found at: http://www.freshplaza.it., (© 2012 The Authors. Molecular Plant Pathology © 2012 BSPP and Blackwell Publishing Ltd.)
- Published
- 2012
- Full Text
- View/download PDF
46. Salvage high-dose chemotherapy in patients with germ cell tumors: an Italian experience with 84 patients.
- Author
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Rosti G, De Giorgi U, Salvioni R, Papiani G, Sebastiani L, Argnani M, Monti G, Ferrante P, Pizzocaro G, and Marangolo M
- Subjects
- Adolescent, Adult, Antineoplastic Combined Chemotherapy Protocols adverse effects, Combined Modality Therapy, Female, Hematopoietic Stem Cell Transplantation, Humans, Male, Middle Aged, Neoplasms, Germ Cell and Embryonal mortality, Survival Rate, Antineoplastic Combined Chemotherapy Protocols administration & dosage, Neoplasms, Germ Cell and Embryonal drug therapy, Salvage Therapy
- Abstract
Background: High-dose chemotherapy (HDCT) followed by hematopoietic stem cell support (HSCS) potentially may be curative in patients with germ cell tumor (GCT) who develop recurrent tumors or who have an inadequate response after receiving standard-dose chemotherapy. The authors report their experience with HDCT as salvage therapy for patients with GCT., Methods: Between 1986 and 2000, 84 patients with GCT, with a median age 29 years (range, 15-50 years), were treated with 105 courses of HDCT with HSCS. Patients were stratified into good, intermediate, and poor risk categories according to a validated prognostic index., Results: Overall, 28 patients (33%) have been continuously disease free. In the good risk group, 24 patients (69%) have been continuously disease free compared with 4 patients (13%) in the intermediate risk group (P < 0.001) and 0 patients in the poor risk group (P < 0.001). Treatment-related mortality occurred only among patients in the poor risk (n = 6 patients) and the intermediate risk groups (n = 4 patients)., Conclusions: In the authors' experience, HDCT induced impressive long-term remissions as salvage treatment among patients in the good risk group. Moreover, the use of validated prognostic classifications may contribute to a better definition of the role of HDCT other than improving the outcome of patients with GCT. The definitive statement on the possible role of HDCT in patients with GCT will derive from the ongoing Phase III randomized studies., (Copyright 2002 American Cancer Society.DOI 10.1002/cncr.10672)
- Published
- 2002
- Full Text
- View/download PDF
47. High-dose chemotherapy for solid tumors: results of the EBMT.
- Author
-
Rosti G, Ferrante P, Ledermann J, Leyvraz S, Ladenstein R, Koscileniak E, Crown J, Dazzi C, Cariello A, and Marangolo M
- Subjects
- Female, Humans, Male, Randomized Controlled Trials as Topic, Antineoplastic Agents administration & dosage, Neoplasms drug therapy
- Abstract
The European Group for Blood and Marrow Transplantation (EBMT), formerly known as European Group for Bone Marrow Transplantation, was established in 1974 in the Netherlands to share experiences, to promote research and clinical studies and to set up registries in the field of hematopoietic tissue transplantation. At the present time more 400 European and non-European centers are members of the EBMT group. In 1984 a new Working Party was created (Solid Tumors) with the aim to investigate the role of high-dose chemotherapy and stem cell support in the fields of adult and pediatric solid tumors. By January 2000 more than 14000 patients were registered, and at the present time this Registry is the world largest database on this subject. Several phase III randomized clinical trials have recently started on behalf of the Group in different diseases (breast carcinoma, small cell lung cancer, ovarian carcinoma, germ cell tumors and Ewing's family sarcoma). Hundreds of randomized patients will finally produce clearer information on this still experimental therapeutic modality. This paper will describe the EBMT Solid Tumors Working Party Registry updated results as well as the main ongoing studies.
- Published
- 2002
- Full Text
- View/download PDF
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