85 results on '"Bates TE"'
Search Results
2. Nitric oxide synthase is present in the cerebrospinal fluid of patients with active multiple sclerosis and is associated with increases in cerebrospinal fluid protein nitrotyrosine and S-nitrosothiols and with changes in glutathione levels
- Author
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Calabrese, V, Scapagnini, Giovanni, Ravagna, A, Bella, R, Foresti, R, Bates, Te, Giuffrida, Stella, Am, and Pennisi, G.
- Published
- 2002
3. Effects of acetyl-l-carnitine on the formation of fatty acid ethyl esters in brain and peripheral organs after short-term ethanol administration in rat
- Author
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Calabrese, V, Scapagnini, Giovanni, Catalano, C, Dinotta, F, Bates, Te, Calvani, M, and Stella, Am
- Published
- 2001
4. Regulation of heat shock protein synthesis in human skin fibroblasts in response to oxidative stress: role of vitamin E
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Calabrese, Vittorio, Scapagnini, G, Catalano, C, Bates, Te, Geraci, Diego, Pennisi, Giovanni, GIUFFRIDA STELLA AM, and TISSUE REACT, INT J.
- Published
- 2001
5. Survival of glioma and colorectal cancer patients using tricyclic antidepressants post-diagnosis.
- Author
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Walker AJ, Grainge M, Bates TE, Card TR, Walker, Alex J, Grainge, Matthew, Bates, Timothy E, and Card, Tim R
- Abstract
Background: Tricyclic antidepressants have been demonstrated in the laboratory to have anticancer properties. A recent study by our group also suggested a protective effect against development of colorectal cancer and glioma. This study aims to determine whether the anticancer action of tricyclics translates to improved survival in these cancers post-diagnosis.Methods: A study using the General Practice Research Database examined whether tricyclic antidepressant exposure in the months following diagnosis of glioma or colorectal cancer would affect longer term all-cause mortality. Cox proportional hazards modelling adjusted for age, gender, smoking, body mass index, comorbidity, and diagnosed depression.Results: A cohort of 1,364 glioma and 16,519 colorectal cancer patients were identified. There was a non-significant reduction in the hazard for glioma patients treated with tricyclics (HR = 0.83, CI = 0.53-1.30). This was mainly found in patients who were not previously exposed to tricyclics (HR = 0.56, CI = 0.26-1.18). In contrast, a significant increase in hazard was found for colorectal cancer (HR = 1.37, CI = 1.21-1.54). This was mostly in patients prescribed low-dose tricyclics (HR = 1.57, CI = 1.33-1.86).Conclusions: We have shown no significant mortality reduction in colorectal cancer or glioma patients treated with tricyclics. An apparent detrimental effect observed in colorectal cancer may be related to prescription of low-dose tricyclics in the management of pain related to disseminated cancer. We cannot rule out small effects or an effect that occurs exclusively at higher doses. Blinded clinical studies may therefore be the only method of determining efficacy in glioma patients. [ABSTRACT FROM AUTHOR]- Published
- 2012
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6. Altered phosphorylation status, phospholipid metabolism and gluconeogenesis in the host liver of rats with prostate cancer: a 31P magnetic resonance spectroscopy study.
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Dagnelie, PC, Bell, JD, Williams, SCR, Bates, TE, Abel, PD, and Foster, CS
- Published
- 1993
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7. Co-inhibition of BCL-XL and MCL-1 with selective BCL-2 family inhibitors enhances cytotoxicity of cervical cancer cell lines.
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Abdul Rahman SF, Muniandy K, Soo YK, Tiew EYH, Tan KX, Bates TE, and Mohana-Kumaran N
- Abstract
Development of resistance to chemo- and radiotherapy in patients suffering from advanced cervical cancer narrows the therapeutic window for conventional therapies. Previously we reported that a combination of the selective BCL-2 family inhibitors ABT-263 and A-1210477 decreased cell proliferation in C33A, SiHa and CaSki human cervical cancer cell lines. As ABT-263 binds to both BCL-2 and BCL-XL with high affinity, it was unclear whether the synergism of the drug combination was driven either by singly inhibiting BCL-2 or BCL-XL, or inhibition of both. In this present study, we used the BCL-2 selective inhibitor ABT-199 and the BCL-XL selective inhibitor A1331852 to resolve the individual antitumor activities of ABT-263 into BCL-2 and BCL-XL dependent mechanisms. A-1210477 was substituted for the orally bioavailable S63845. Four cervical cancer cell lines were treated with the selective BCL-2 family inhibitors ABT-199, A1331852 and S63845 alone and in combination using 2-dimensional (2D) and 3-dimensional (3D) cell culture models. The SiHa, C33A and CaSki cell lines were resistant to single agent treatment of all three drugs, suggesting that none of the BCL-2 family of proteins mediate survival of the cells in isolation. HeLa cells were resistant to single agent treatment of ABT-199 and A1331852 but were sensitive to S63845 indicating that they depend on MCL-1 for survival. Co-inhibition of BCL-2 and MCL-1 with ABT-199 and S63845, inhibited cell proliferation of all cancer cell lines, except SiHa. However, the effect of the combination was not as pronounced as combination of A1331852 and S63845. Co-inhibition of BCL-XL and MCL-1 with A1331852 and S63845 significantly inhibited cell proliferation of all four cell lines. Similar data were obtained with 3-dimensional spheroid cell culture models generated from two cervical cancer cell lines in vitro . Treatment with a combination of A1331852 and S63845 resulted in inhibition of growth and invasion of the 3D spheroids. Collectively, our data demonstrate that the combination of MCL-1-selective inhibitors with either selective inhibitors of either BCL-XL or BCL-2 may be potentially useful as treatment strategies for the management of cervical cancer., Competing Interests: The authors declare no conflicts of interest., (© 2020 The Authors.)
- Published
- 2020
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8. Anti-angiogenic drugs: direct anti-cancer agents with mitochondrial mechanisms of action.
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Quayle LA, Pereira MG, Scheper G, Wiltshire T, Peake RE, Hussain I, Rea CA, and Bates TE
- Abstract
Components of the mitochondrial electron transport chain have recently gained much interest as potential therapeutic targets. Since mitochondria are essential for the supply of energy that is required for both angiogenic and tumourigenic activity, targeting the mitochondria represents a promising potential therapeutic approach for treating cancer. Here we investigate the established anti-angiogenesis drugs combretastatin A4, thalidomide, OGT 2115 and tranilast that we hypothesise are able to exert a direct anti-cancer effect in the absence of vasculature by targeting the mitochondria. Drug cytotoxicity was measured using the MTT assay. Mitochondrial function was measured in intact isolated mitochondria using polarography, fluorimetry and enzymatic assays to measure mitochondrial oxygen consumption, membrane potential and complex I-IV activities respectively. Combretastatin A4, OGT 2115 and tranilast were both shown to decrease mitochondrial oxygen consumption. OGT 2115 and tranilast decreased mitochondrial membrane potential and reduced complex I activity while combretastatin A4 and thalidomide did not. OGT 2115 inhibited mitochondrial complex II-III activity while combretastatin A4, thalidomide and tranilast did not. Combretastatin A4, thalidomide and OGT 2115 induced bi-phasic concentration-dependent increases and decreases in mitochondrial complex IV activity while tranilast had no evident effect. These data demonstrate that combretastatin A4, thalidomide, OGT 2115 and tranilast are all mitochondrial modulators. OGT 2115 and tranilast are both mitochondrial inhibitors capable of eliciting concentration-dependent reductions in cell viability by decreasing mitochondrial membrane potential and oxygen consumption., Competing Interests: CONFLICTS OF INTEREST The authors declare that there are no conflicts of interest.
- Published
- 2017
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9. The effects of antidepressants on mitochondrial function in a model cell system and isolated mitochondria.
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Abdel-Razaq W, Kendall DA, and Bates TE
- Subjects
- Animals, CHO Cells, Clomipramine pharmacology, Cricetinae, Cricetulus, Desipramine pharmacology, Electron Transport Chain Complex Proteins metabolism, Fluoxetine analogs & derivatives, Fluoxetine pharmacology, Male, Membrane Potential, Mitochondrial drug effects, Membrane Potential, Mitochondrial physiology, Mitochondria, Heart metabolism, Oxygen Consumption, Rats, Rats, Wistar, Thiazepines pharmacology, Antidepressive Agents pharmacology, Mitochondria, Heart drug effects
- Abstract
The in vitro effects of antidepressant drugs on mitochondrial function were investigated in a CHOβ(2)SPAP cell line used previously to determine the effects of antidepressants on gene transcription (Abdel-Razaq et al., Biochem Pharmacol 73:1995-2003, 2007) and in rat heart isolated mitochondria. Apoptotic effects of clomipramine (CLOM), desipramine (DMI) and of norfluoxetine (NORF, the active metabolite of fluoxetine), on cellular viability were indicated by morphological changes and concentration-dependent increases in caspase-3 activity in CHO cells after 18 h exposure to CLOM, DMI and NORF. However, tianeptine (TIAN) was without effect. CLOM and NORF both reduced integrated mitochondrial function as shown by marked reductions in membrane potential (MMP) in mitochondria isolated from rat hearts. DMI also showed a similar but smaller effect, whereas, TIAN did not elicit any significant change in MMP. Moreover, micromolar concentrations of CLOM, DMI and NORF caused significant inhibitions of the activities of mitochondrial complexes (I, II/III and IV). The inhibitory effects on complex IV activity were most marked. TIAN inhibited only complex I activity at concentrations in excess of 20 μM. The observed inhibitory effects of antidepressants on the mitochondrial complexes were accompanied by a significant decrease in the mitochondrial state-3 respiration at concentrations above 10 μM. The results demonstrate that the apoptotic cell death observed in antidepressant-treated cells could be due to disruption of mitochondrial function resulting from multiple inhibition of mitochondrial enzyme complexes. The possibility that antimitochondrial actions of antidepressants could provide a potentially protective pre-conditioning effect is discussed.
- Published
- 2011
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10. Tricyclic antidepressants and the incidence of certain cancers: a study using the GPRD.
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Walker AJ, Card T, Bates TE, and Muir K
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- Aged, Case-Control Studies, Databases, Factual, Female, Follow-Up Studies, Humans, Incidence, Male, Middle Aged, Neoplasms pathology, Prospective Studies, Risk Factors, United Kingdom epidemiology, Antidepressive Agents, Tricyclic adverse effects, Depression chemically induced, Neoplasms drug therapy, Neoplasms epidemiology
- Abstract
Background: Several studies suggest links between cancer and tricyclic antidepressant use., Methods: A case-control study using the General Practice Research Database examined whether previous tricyclic usage was associated with reduced incidence of brain (with glioma as a sub-category), breast, colorectal, lung and prostate cancers. Conditional logistic regression adjusted for age, gender, general practice, depression, smoking, body mass index, alcohol use and non-steroidal anti-inflammatory drug use., Results: A total of 31 953 cancers were identified, each matched with up to two controls. We found a statistically significant reduction in tricyclic prescriptions compared with controls in glioma (odds ratio (OR) =0.59, 95% confidence interval (CI)=0.42-0.81) and colorectal cancer patients (OR=0.84, CI=0.75-0.94). These effects were dose-dependent (P-values for trend, glioma=0.0005, colorectal=0.001) and time-dependant (P-values for trend glioma=0.0005, colorectal=0.0086). The effects were cancer-type specific, with lung, breast and prostate cancers largely unaffected by antidepressant use., Conclusion: The biologically plausible, specific and dose- and time-dependant inverse association that we have found suggests that tricyclics may have potential for prevention of both colorectal cancer and glioma.
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- 2011
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11. Predictive value of tumor proliferative indices in periampullary cancers: Ki-67, mitotic activity index (MI) and volume corrected mitotic index (M/V) using tissue microarrays.
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Aloysius MM, Hewavisenthi SJ, Bates TE, Rowlands BJ, Lobo DN, and Zaitoun AM
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- Bile Duct Neoplasms mortality, Bile Duct Neoplasms pathology, Carcinoma, Pancreatic Ductal mortality, Carcinoma, Pancreatic Ductal pathology, Cholangiocarcinoma mortality, Cholangiocarcinoma pathology, Common Bile Duct Neoplasms mortality, Common Bile Duct Neoplasms pathology, Duodenal Neoplasms mortality, Duodenal Neoplasms pathology, Humans, Immunohistochemistry, Lymphatic Metastasis, Microarray Analysis, Neoplasm Invasiveness, Pancreatic Neoplasms mortality, Pancreatic Neoplasms pathology, Ampulla of Vater, Bile Duct Neoplasms metabolism, Bile Ducts, Intrahepatic, Carcinoma, Pancreatic Ductal metabolism, Cholangiocarcinoma metabolism, Common Bile Duct Neoplasms metabolism, Duodenal Neoplasms metabolism, Ki-67 Antigen analysis, Mitotic Index, Pancreatic Neoplasms metabolism
- Abstract
Background: Morphometry [nuclear Ki-67 labelling, mitotic activity index (MI), and volume-corrected mitotic index (M/V)] for periampullary cancers using tissue microarrays has not been performed previously. The purpose of the study was to assess these indices on tissue microarray (TMA) sections constructed from patients with periampullary cancers and study their association with clinicopathological variables., Methods: Immunohistochemical staining for Ki-67 was performed on formalin-fixed pancreatic TMA sections. Expression of Ki-67 was assessed as the percentage of cancer cell nuclei expressing MIB1, MI as the mean percentage of Ki-67 from 10 random high-power fields, and M/V was calculated after standardizing MI for connective tissue volume and microscope parameters in the tumor using established protocols., Results: Patients > or =70 years with periampullary cancers had higher Ki-67 expression (>15) compared with patients <70 years of age (chi(2) = 3.9, P = 0.047). Ki-67 expression was higher in tumors > or =2 cm (chi(2) = 4.9, P = 0.028) compared with smaller tumors. Higher MI (>15) was clearly associated with worsening histological grade (chi(2) = 9.2, P = 0.010). The median survival for tumors of the pancreaticobiliary subtype (pancreatic ductal adenocarcinoma and cholangiocarcinoma) was 43 months in the group with an M/V score of <20, compared with 18 months for the group with a score > or =20 (P = 0.001). There was no statistically significant difference in survival, based on M/V score, for tumors of the intestinal subtype (ampullary and duodenal adenocarcinoma)., Conclusions: In periampullary cancers, Ki-67 and MI are proliferative indices predictive of tumor behavior. M/V was predictive of survival in tumors of the pancreaticobiliary subtype.
- Published
- 2010
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12. Immunohistochemical expression of mitochondrial membrane complexes (MMCs) I, III, IV and V in malignant and benign periampullary epithelium: a potential target for drug therapy of periampullary cancer?
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Aloysius MM, Zaitoun AM, Bates TE, Ilyas M, Constantin-Teodosiu D, Rowlands BJ, and Lobo DN
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- Adult, Aged, Cell Proliferation, Epithelium drug effects, Female, Humans, Lymphatic Metastasis, Male, Middle Aged, Neoplasm Metastasis, Oxidative Stress, Pancreas pathology, Pancreatic Neoplasms therapy, Pancreatitis pathology, Phosphorylation, Retrospective Studies, Antineoplastic Agents pharmacology, Immunohistochemistry methods, Mitochondria metabolism, Pancreatic Neoplasms metabolism
- Abstract
Background: Mitochondrial membrane complexes (MMCs) are key mediators of cellular oxidative phosphorylation, and inhibiting them could lead to cell death. No published data are available on the relative abundance of MMCs in different periampullary cancers. Therefore, we studied the expression profile of MMCs I, III, IV and V in periampullary cancers, reactive pancreatitis, normal pancreas and chronic pancreatitis., Methods: This was a retrospective study on tissue microarrays constructed from formalin-fixed paraffin-embedded tissue from 126 consecutive patients (cancer = 104, chronic pancreatitis = 22) undergoing pancreatic resections between June 2001 and June 2006. 78 specimens of chronic pancreatitis tissue were obtained adjacent to areas of cancer. Normal pancreatic tissue was obtained from the resection specimens in a total of 30 patients. Metastatic tumours in 61 regional lymph nodes from 61 patients were also studied., Results: MMCs I, III, IV and V were highly expressed (p < 0.05) in all primary periampullary cancers compared with metastatic lymph nodes and adjacent benign pancreas. MMCs III, IV and V were highly expressed in all cancers regardless of type compared with chronic pancreatitis (p < 0.05). Higher expression of MMCs I and V was associated with better survival and may, in part, relate to lower expression of these MMCs in poorly differentiated tumours compared with well and moderately differentiated tumours., Conclusions: Differential expression of MMCs III, IV and V in primary periampullary cancers compared with adjacent benign periampullary tissue and chronic pancreatitis is a novel finding, which may render them attractive anticancer targets.
- Published
- 2010
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13. Complete absence of M2-pyruvate kinase expression in benign pancreatic ductal epithelium and pancreaticobiliary and duodenal neoplasia.
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Aloysius MM, Zaitoun AM, Bates TE, Albasri A, Ilyas M, Rowlands BJ, and Lobo DN
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- Aged, Carcinoma, Pancreatic Ductal genetics, Carcinoma, Pancreatic Ductal pathology, Duodenal Neoplasms genetics, Duodenal Neoplasms pathology, Epithelium enzymology, Epithelium pathology, Female, Humans, Male, Middle Aged, Pancreas cytology, Pancreas enzymology, Pancreas pathology, Pancreatic Neoplasms genetics, Pancreatic Neoplasms pathology, Pyruvate Kinase metabolism, Carcinoma, Pancreatic Ductal enzymology, Duodenal Neoplasms enzymology, Gene Expression Regulation, Neoplastic, Pancreatic Neoplasms enzymology, Pyruvate Kinase genetics
- Abstract
Background: Elevated serum concentrations of M2-pyruvate kinase (M2-PK) correlate with poor prognosis in patients with pancreaticobiliary and duodenal cancer, but the expression of M2-PK in formalin-fixed pancreatic tissue is unknown. We aimed to characterise the immunohistochemical expression of M2-PK in archived specimens of pancreaticobiliary and duodenal cancers, premalignant lesions, chronic pancreatitis, and normal pancreas., Methods: Immunohistochemical staining was performed with mouse anti-M2-PK monoclonal antibody (clone DF-4) at an optimal dilution of 1:25 on tissue microarrays constructed from formalin-fixed paraffin-embedded pancreatic tissue of 126 consecutive patients undergoing pancreatic resections between June 2001 and June 2006. 104 underwent resection for cancer and 22 for chronic pancreatitis. 78 specimens of chronic pancreatitis tissue were obtained adjacent to areas of cancer. Normal pancreatic tissue was obtained from the resection specimens in a total of 30 patients. Metastatic tumours in 61 regional lymph nodes from 61 patients were also studied. A further 11 premalignant pancreaticobiliary and duodenal lesions were studied. M2-PK expression was quantified with the immunohistochemical score (IHS; Range 0-12)., Results: Benign non-ductal tissue in chronic pancreatitis and normal pancreas showed variable expression of M2-PK (IHS = 1 in 25%, IHS = 2-3 in 40%, IHS>3 in 40%). Benign pancreatic ductal epithelium, all primary pancreaticobiliary and duodenal premalignant lesions and cancers (and lymph node metastasis) showed complete lack of expression (IHS = 0)., Conclusion: Complete lack of M2-PK expression was observed in benign pancreatic ducts, premalignant lesions and cancer. M2-PK is present only in benign non-ductal epithelium in normal pancreas and peri-tumoural tissue.
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- 2009
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14. Repeated novel cage exposure-induced improvement of early Alzheimer's-like cognitive and amyloid changes in TASTPM mice is unrelated to changes in brain endocannabinoids levels.
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Pardon MC, Sarmad S, Rattray I, Bates TE, Scullion GA, Marsden CA, Barrett DA, Lowe J, and Kendall DA
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- Alzheimer Disease physiopathology, Amyloid beta-Peptides analysis, Amyloid beta-Protein Precursor genetics, Amyloid beta-Protein Precursor metabolism, Animals, Brain pathology, Brain physiopathology, Cannabinoid Receptor Modulators analysis, Cognition Disorders metabolism, Cognition Disorders physiopathology, Cognition Disorders therapy, Disease Models, Animal, Environment, Controlled, Exploratory Behavior physiology, Housing, Animal, Memory, Short-Term physiology, Mice, Mice, Transgenic, Motor Activity physiology, Presenilin-1 genetics, Presenilin-1 metabolism, Stress, Psychological complications, Stress, Psychological metabolism, Stress, Psychological physiopathology, Alzheimer Disease metabolism, Alzheimer Disease therapy, Amyloid beta-Peptides metabolism, Brain metabolism, Cannabinoid Receptor Modulators metabolism, Endocannabinoids
- Abstract
Environmental factors (e.g. stress, exercise, enrichment) are thought to play a role in the development of Alzheimer's disease later in life. We investigated the influence of repeated novel cage exposure on the development of early Alzheimer's-like pathology in adult (4 months old) double transgenic mice over-expressing the amyloid precursor protein and presenilin-1 genes (TASTPM mouse line). The procedure involves the repeated placement of the animal into a novel clean cage, a manipulation which induces a stress response and exploratory activity and, as such, can also be seen as a mild form of enrichment. Before and after exposure to the novel cage procedure, separate groups of mice were evaluated for locomotor performance and short-term contextual memory in the fear-conditioning test. Repeated novel cage exposure prevented the onset of a short-term memory deficit that was apparent in 5.5- but not 4-month-old TASTPM mice, without reversing the deficit in extinction already evident at 4 months of age. Brain regional levels of soluble and insoluble amyloid and of endocannabinoids were quantified. Novel cage exposure attenuated soluble and insoluble amyloid accumulation in the hippocampus and frontal cortex, without affecting the age-related increases in regional brain endocannabinoids levels. These beneficial effects are likely to be the consequence of the increase in physical and exploratory activity induced by novel cage exposure and suggest that the impact of environmental factors on Alzheimer's-like changes may be dependent on the degree of activation of stress pathways.
- Published
- 2009
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15. Cellular stress response: a novel target for chemoprevention and nutritional neuroprotection in aging, neurodegenerative disorders and longevity.
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Calabrese V, Cornelius C, Mancuso C, Pennisi G, Calafato S, Bellia F, Bates TE, Giuffrida Stella AM, Schapira T, Dinkova Kostova AT, and Rizzarelli E
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- Animals, Homeostasis, Humans, Reactive Oxygen Species metabolism, Aging, Diet, Longevity, Neurodegenerative Diseases metabolism, Oxidative Stress
- Abstract
The predominant molecular symptom of aging is the accumulation of altered gene products. Moreover, several conditions including protein, lipid or glucose oxidation disrupt redox homeostasis and lead to accumulation of unfolded or misfolded proteins in the aging brain. Alzheimer's and Parkinson's diseases or Friedreich ataxia are neurological diseases sharing, as a common denominator, production of abnormal proteins, mitochondrial dysfunction and oxidative stress, which contribute to the pathogenesis of these so called "protein conformational diseases". The central nervous system has evolved the conserved mechanism of unfolded protein response to cope with the accumulation of misfolded proteins. As one of the main intracellular redox systems involved in neuroprotection, the vitagene system is emerging as a neurohormetic potential target for novel cytoprotective interventions. Vitagenes encode for cytoprotective heat shock proteins (Hsp) Hsp70 and heme oxygenase-1, as well as thioredoxin reductase and sirtuins. Nutritional studies show that ageing in animals can be significantly influenced by dietary restriction. Thus, the impact of dietary factors on health and longevity is an increasingly appreciated area of research. Reducing energy intake by controlled caloric restriction or intermittent fasting increases lifespan and protects various tissues against disease. Genetics has revealed that ageing may be controlled by changes in intracellular NAD/NADH ratio regulating sirtuin, a group of proteins linked to aging, metabolism and stress tolerance in several organisms. Recent findings suggest that several phytochemicals exhibit biphasic dose responses on cells with low doses activating signaling pathways that result in increased expression of vitagenes encoding survival proteins, as in the case of the Keap1/Nrf2/ARE pathway activated by curcumin and NAD/NADH-sirtuin-1 activated by resveratrol. Consistently, the neuroprotective roles of dietary antioxidants including curcumin, acetyl-L-carnitine and carnosine have been demonstrated through the activation of these redox-sensitive intracellular pathways. Although the notion that stress proteins are neuroprotective is broadly accepted, still much work needs to be done in order to associate neuroprotection with specific pattern of stress responses. In this review the importance of vitagenes in the cellular stress response and the potential use of dietary antioxidants in the prevention and treatment of neurodegenerative disorders is discussed.
- Published
- 2008
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16. Protection of respiratory chain enzymes from ischaemic damage in adult rat brain slices.
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Brooks KJ, Hargreaves IP, and Bates TE
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- Animals, Brain Ischemia metabolism, Citrate (si)-Synthase metabolism, Electron Transport physiology, Female, Male, Mitochondria metabolism, Rats, Brain Ischemia pathology, Electron Transport Complex I metabolism, Electron Transport Complex II metabolism, Electron Transport Complex III metabolism, Electron Transport Complex IV metabolism
- Abstract
The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused adult cortical brain slices. After 15 min of AH the activity of complex II-III was significantly reduced (by 45%) with no change in complex I or IV. Following 30 min of reperfusion the activities of complex II-III and IV were significantly reduced (by 45% and 20% respectively). These reductions in enzyme activity were abolished by removing the external calcium or by the addition of N omega-nitro-L-arginine (LNNA) or an analogue of superoxide dismutase (SOD) manganese [III] tetrakis 4-benzoic acid porphyrin (Mn-TBAP). These data suggest that a reactive oxygen species (ROS) such as peroxynitrite is involved in the reduction of mitochondrial complex activities following AH.
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- 2008
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17. Curcumin and the cellular stress response in free radical-related diseases.
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Calabrese V, Bates TE, Mancuso C, Cornelius C, Ventimiglia B, Cambria MT, Di Renzo L, De Lorenzo A, and Dinkova-Kostova AT
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- Animals, Antineoplastic Agents therapeutic use, Diabetes Mellitus drug therapy, Disease Models, Animal, Food Coloring Agents, Humans, Hypoglycemic Agents therapeutic use, India, Mice, Mice, Knockout, NF-E2-Related Factor 2 deficiency, NF-E2-Related Factor 2 drug effects, NF-E2-Related Factor 2 genetics, Neoplasms drug therapy, Neurodegenerative Diseases drug therapy, Taste, Curcumin therapeutic use, Free Radicals metabolism, Stress, Physiological drug effects
- Abstract
Free radicals play a main pathogenic role in several human diseases such as neurodegenerative disorders, diabetes, and cancer. Although there has been progress in treatment of these diseases, the development of important side effects may complicate the therapeutic course. Curcumin, a well known spice commonly used in India to make foods colored and flavored, is also used in traditional medicine to treat mild or moderate human diseases. In the recent years, a growing body of literature has unraveled the antioxidant, anticarcinogenic, and antinfectious activity of curcumin based on the ability of this compound to regulate a number of cellular signal transduction pathways. These promising data obtained in vitro are now being translated to the clinic and more than ten clinical trials are currently ongoing worldwide. This review outlines the biological activities of curcumin and discusses its potential use in the prevention and treatment of human diseases.
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- 2008
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18. Cannabinoid receptor agonists are mitochondrial inhibitors: a unified hypothesis of how cannabinoids modulate mitochondrial function and induce cell death.
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Athanasiou A, Clarke AB, Turner AE, Kumaran NM, Vakilpour S, Smith PA, Bagiokou D, Bradshaw TD, Westwell AD, Fang L, Lobo DN, Constantinescu CS, Calabrese V, Loesch A, Alexander SP, Clothier RH, Kendall DA, and Bates TE
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- Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, Electron Transport Complex I drug effects, Electron Transport Complex II drug effects, Electron Transport Complex III drug effects, Endocannabinoids, Humans, Hydrogen Peroxide metabolism, Lung Neoplasms, Membrane Potential, Mitochondrial drug effects, Models, Biological, Oxygen Consumption drug effects, Apoptosis drug effects, Arachidonic Acids pharmacology, Cannabinoid Receptor Agonists, Cannabinoids pharmacology, Dronabinol analogs & derivatives, Dronabinol pharmacology, Mitochondria drug effects, Mitochondria physiology, Polyunsaturated Alkamides pharmacology
- Abstract
Time-lapse microscopy of human lung cancer (H460) cells showed that the endogenous cannabinoid anandamide (AEA), the phyto-cannabinoid Delta-9-tetrahydrocannabinol (THC) and a synthetic cannabinoid HU 210 all caused morphological changes characteristic of apoptosis. Janus green assays of H460 cell viability showed that AEA and THC caused significant increases in OD 595 nm at lower concentrations (10-50 microM) and significant decreases at 100 microM, whilst HU 210 caused significant decreases at all concentrations. In rat heart mitochondria, all three ligands caused significant decreases in oxygen consumption and mitochondrial membrane potential. THC and HU 210 caused significant increases in mitochondrial hydrogen peroxide production, whereas AEA was without significant effect. All three ligands induced biphasic changes in either mitochondrial complex I activity and/or mitochondrial complex II-III activity. These data demonstrate that AEA, THC, and HU 210 are all able to cause changes in integrated mitochondrial function, directly, in the absence of cannabinoid receptors.
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- 2007
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19. Natural antioxidants in Alzheimer's disease.
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Mancuso C, Bates TE, Butterfield DA, Calafato S, Cornelius C, De Lorenzo A, Dinkova Kostova AT, and Calabrese V
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- Acetylcarnitine chemistry, Acetylcarnitine pharmacology, Acetylcarnitine therapeutic use, Alzheimer Disease metabolism, Alzheimer Disease pathology, Animals, Antioxidants chemistry, Antioxidants pharmacology, Cholinesterase Inhibitors chemistry, Cholinesterase Inhibitors pharmacology, Cholinesterase Inhibitors therapeutic use, Humans, Neuroprotective Agents chemistry, Neuroprotective Agents pharmacology, Neuroprotective Agents therapeutic use, Oxidative Stress drug effects, Oxidative Stress physiology, Resveratrol, Stilbenes chemistry, Stilbenes pharmacology, Stilbenes therapeutic use, Alzheimer Disease drug therapy, Antioxidants therapeutic use
- Abstract
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterised by severe cognitive impairment that ultimately leads to death. Current drugs used in AD are acetylcholinesterase inhibitors and antagonists to the NMDA receptors. These drugs may only slightly improve cognitive functions but have only very limited impact on the clinical course of the disease. In the past several years, based on in vitro and in vivo studies in laboratory animals, natural antioxidants, such as resveratrol, curcumin and acetyl-L-carnitine have been proposed as alternative therapeutic agents for AD. An increasing number of studies demonstrated the efficacy of primary antioxidants, such as polyphenols, or secondary antioxidants, such as acetylcarnitine, to reduce or to block neuronal death occurring in the pathophysiology of this disorder. These studies revealed that other mechanisms than the antioxidant activities could be involved in the neuroprotective effect of these compounds. This paper discusses the evidence for the role of acetylcarnitine in modulating redox-dependent mechanisms leading to the upregulation of vitagenes. Furthermore, future development of novel antioxidant drugs targeted to the mitochondria should result in effectively slowing disease progression. The association with new drug delivery systems may be desirable and useful for the therapeutic use of antioxidants in human neurodegenerative diseases.
- Published
- 2007
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20. The effects of antidepressants on cyclic AMP-response element-driven gene transcription in a model cell system.
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Abdel-Razaq W, Bates TE, and Kendall DA
- Subjects
- Alkaline Phosphatase biosynthesis, Alkaline Phosphatase genetics, Animals, Blotting, Western, CHO Cells, Clomipramine pharmacology, Cricetinae, Cricetulus, Desipramine pharmacology, Dose-Response Relationship, Drug, Fluoxetine analogs & derivatives, Fluoxetine pharmacology, Genes, Reporter, Humans, Receptors, Adrenergic, beta-2 genetics, Receptors, Adrenergic, beta-2 metabolism, Thiazepines pharmacology, Time Factors, Antidepressive Agents, Tricyclic pharmacology, Cyclic AMP Response Element-Binding Protein metabolism, Transcription, Genetic drug effects
- Abstract
The effects of the antidepressant drugs clomipramine (CLOM), desipramine (DMI), tianeptine (TIAN) and of norfluoxetine (NORF, the active metabolite of fluoxetine), were investigated in CHO cells expressing human beta2 adrenoceptors and a secreted placental alkaline phosphatase (SPAP) reporter gene to determine their actions on cyclic AMP-driven gene transcription. After 18 h of exposure, CLOM, DMI and NORF, but not TIAN, had biphasic effects on 1 microM isoprenaline-stimulated SPAP fsproduction with concentrations between 10 nM and 1 microM enhancing the maximal (E(max)) SPAP response, without changing EC50 values, but higher concentrations produced marked inhibitory effects. At nanomolar concentrations, CLOM and DMI increased expression of phospho-CREB (cyclic AMP response element binding protein). NORF was less effective but did significantly increase phospho-CREB at a concentration of 200 nM. TIAN had no effect. None of the antidepressants had any effect on CREB expression, nor on the accumulation of cyclic AMP. After prolonged exposure (7-21 days) to a low concentration (200 nM) of the antidepressants, the enhanced E(max) values for SPAP production evident after 18 h were not maintained but CLOM and DMI induced a significant leftward shift in the isoprenaline EC50 after a 7-day period of treatment and this was sustained at the 21 day time point. TIAN did not produce any significant changes. The results demonstrate that, in vitro, some but not all antidepressants can modify gene transcription via monoamine and cyclic AMP-independent mechanisms. The in vivo adaptive responses to TIAN probably involve alterations in different gene sets to those affected by other antidepressants.
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- 2007
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21. Vanilloid receptor agonists and antagonists are mitochondrial inhibitors: how vanilloids cause non-vanilloid receptor mediated cell death.
- Author
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Athanasiou A, Smith PA, Vakilpour S, Kumaran NM, Turner AE, Bagiokou D, Layfield R, Ray DE, Westwell AD, Alexander SP, Kendall DA, Lobo DN, Watson SA, Lophatanon A, Muir KA, Guo DA, and Bates TE
- Subjects
- Anilides administration & dosage, Animals, Capsaicin analogs & derivatives, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung metabolism, Carcinoma, Non-Small-Cell Lung pathology, Cells, Cultured, Cinnamates administration & dosage, Diterpenes administration & dosage, Dose-Response Relationship, Drug, Humans, Hydrogen Peroxide metabolism, Lung Neoplasms drug therapy, Lung Neoplasms metabolism, Lung Neoplasms pathology, Membrane Potentials drug effects, Mitochondria, Heart metabolism, Mitochondria, Heart pathology, Oxygen Consumption drug effects, Rats, TRPV Cation Channels drug effects, TRPV Cation Channels metabolism, Anilides pharmacology, Apoptosis drug effects, Capsaicin pharmacology, Cinnamates pharmacology, Diterpenes pharmacology, Mitochondria, Heart drug effects, TRPV Cation Channels antagonists & inhibitors
- Abstract
Time-lapse photomicroscopy of human H460 lung cancer cells demonstrated of the transient receptor potential V1 (TRPV1) channel agonists, (E)-capsaicin and resiniferatoxin, and the TRPV1 antagonists, capsazepine, and SB366791, were able to bring about morphological changes characteristic of apoptosis and/or necrosis. Immunoblot analysis identified immunoreactivity for the transient receptor potential V1 (TRPV1) channel in rat brain samples, but not in rat heart mitochondria or in H460 cells. In isolated rat heart mitochondria, all four ligands caused concentration-dependent decreases in oxygen consumption and mitochondrial membrane potential. (E)-Capsaicin and capsazepine evoked concentration-dependent increases and decreases, respectively, in mitochondrial hydrogen peroxide production, whilst resiniferatoxin and SB366791 were without significant effect. These data support the hypothesis that (E)-capsaicin, resiniferatoxin, capsazepine, and SB366791 are all mitochondrial inhibitors, able to activate apoptosis and/or necrosis via non-receptor mediated mechanisms, and also support the use of TRPV1 ligands as anti-cancer agents.
- Published
- 2007
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22. Dopamine D2 and D3 receptor agonists limit oligodendrocyte injury caused by glutamate oxidative stress and oxygen/glucose deprivation.
- Author
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Rosin C, Colombo S, Calver AA, Bates TE, and Skaper SD
- Subjects
- Animals, Cell Death drug effects, Cell Death physiology, Cells, Cultured, Dopamine Antagonists pharmacology, Glucose metabolism, Hypoxia-Ischemia, Brain physiopathology, Hypoxia-Ischemia, Brain prevention & control, Oligodendroglia drug effects, Oxidative Stress drug effects, Oxygen metabolism, RNA, Messenger metabolism, Rats, Rats, Sprague-Dawley, Receptors, Dopamine metabolism, Receptors, Dopamine D2 genetics, Receptors, Dopamine D2 metabolism, Receptors, Dopamine D3 genetics, Receptors, Dopamine D3 metabolism, Dopamine Agonists pharmacology, Glutamic Acid metabolism, Hypoxia-Ischemia, Brain metabolism, Oligodendroglia metabolism, Oxidative Stress physiology, Receptors, Dopamine genetics
- Abstract
Dopamine receptor activation is thought to contribute adversely to several neuropathological disorders, including Parkinson's disease and schizophrenia. In addition, dopamine may have a neuroprotective role: dopamine receptor agonists are reported to protect nerve cells by virtue of their antioxidant properties as well as by receptor-mediated mechanisms. White matter injury can also be a significant factor in neurological disorders. Using real-time RT-PCR, we show that differentiated rat cortical oligodendrocytes express dopamine D2 receptor and D3 receptor mRNA. Oligodendrocytes were vulnerable to oxidative glutamate toxicity and to oxygen/glucose deprivation injury. Agonists for dopamine D2 and D3 receptors provided significant protection of oligodendrocytes against these two forms of injury, and the protective effect was diminished by D2 and D3 antagonists. Levels of oligodendrocyte D2 receptor and D3 receptor protein, as measured by Western blotting, appeared to increase following combined oxygen and glucose deprivation. Our results suggest that dopamine D2 and D3 receptor activation may play an important role in oligodendrocyte protection against oxidative glutamate toxicity and oxygen-glucose deprivation injury., (Copyright 2005 Wiley-Liss, Inc.)
- Published
- 2005
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23. The effects of desmethylimipramine on cyclic AMP-stimulated gene transcription in a model cell system.
- Author
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Richards JK, Abdel-Razaq W, Bates TE, and Kendall DA
- Subjects
- Alkaline Phosphatase, Animals, CHO Cells, Calcium metabolism, Cricetinae, Cyclic AMP Response Element-Binding Protein metabolism, Dose-Response Relationship, Drug, Fluoxetine analogs & derivatives, Fluoxetine pharmacology, GPI-Linked Proteins, Isoenzymes biosynthesis, Phosphorylation, Antidepressive Agents, Tricyclic pharmacology, Cyclic AMP physiology, Desipramine pharmacology, Transcription, Genetic drug effects
- Abstract
The present study utilised an in vitro cell model of the cAMP signalling pathway to investigate the actions of desipramine (DMI) and other psychoactive agents on cAMP-driven gene transcription. The model comprised CHObeta2 SPAP cells; Chinese hamster ovary cells expressing human beta2 adrenoceptors and a secreted placental alkaline phosphatase (SPAP) reporter gene with multiple cAMP response elements (CREs) in its promoter region. SPAP assays showed DMI to inhibit isoprenaline or forskolin-enhanced gene transcription in a time and concentration-dependent manner (IC50=16.6+/-2.0 microM after 18 h). This effect of DMI was not dependent upon activity at the levels of the beta2 receptor, cAMP accumulation or phosphorylation of the transcription factor, cAMP response element binding protein (CREB). The inhibitory effects were maintained in the presence of DMI for at least 3 weeks and were mimicked by exposure to norfluoxetine (the major metabolite of fluoxetine; IC50=7.2+/-1.8 microM) and the neuroleptics, chlorpromazine and clozapine, all at a concentration of 10 microM. Amphetamine (10 microM, 18 h) enhanced SPAP gene transcription. Ca2+ imaging experiments ruled out an inhibitory effect of DMI on Ca2+ influx as concluded by previous studies. The results suggest a molecular target for DMI that lies downstream of CREB phosphorylation. Whether the inhibitory action of DMI is common to naturally expressed CRE-driven genes involved in adaptive responses to antidepressants in vivo remains to be determined.
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- 2005
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24. Chlorimipramine: a novel anticancer agent with a mitochondrial target.
- Author
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Daley E, Wilkie D, Loesch A, Hargreaves IP, Kendall DA, Pilkington GJ, and Bates TE
- Subjects
- Animals, Brain Neoplasms drug therapy, Brain Neoplasms metabolism, Brain Neoplasms pathology, Cell Respiration drug effects, Cell Size drug effects, Cell Survival drug effects, Dose-Response Relationship, Drug, Electron Transport Complex III antagonists & inhibitors, Electron Transport Complex III metabolism, Glioma drug therapy, Humans, Male, Membrane Potentials drug effects, Oxygen Consumption drug effects, Rats, Rats, Wistar, Tumor Cells, Cultured, Antineoplastic Agents administration & dosage, Clomipramine administration & dosage, Glioma metabolism, Glioma pathology, Mitochondria drug effects, Mitochondria pathology
- Abstract
Mitochondria have been suggested to be a potential intracellular target for cancer chemotherapy. In this report, we demonstrate the ability of the tricyclic antidepressant chlorimipramine to kill human glioma cells in vitro by a molecular mechanism resulting in an increase in caspase 3 activity following inhibition of glioma oxygen consumption. Studies with isolated rat mitochondria showed that chlorimipramine specifically inhibited mitochondrial complex III activity, which causes decreased mitochondrial membrane potential as well as mitochondrial swelling and vacuolation. The use of chlorimipramine in human as an effective, non-toxic cancer therapeutic having a strong selectivity between cancer cells and normal cells on the basis of their mitochondrial function is discussed.
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- 2005
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25. Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms.
- Author
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Rosin C, Bates TE, and Skaper SD
- Subjects
- 2',3'-Cyclic-Nucleotide Phosphodiesterases metabolism, Animals, Animals, Newborn, Annexin A5 metabolism, Blotting, Western methods, Cell Survival drug effects, Cells, Cultured, Chromatin Assembly and Disassembly drug effects, Cystine pharmacology, Enzyme Inhibitors pharmacology, Excitatory Amino Acid Antagonists pharmacology, Fluoresceins, G(M1) Ganglioside metabolism, Glial Fibrillary Acidic Protein metabolism, Glucose deficiency, Glutamic Acid analogs & derivatives, Glutamic Acid pharmacology, Hypoxia, Immunohistochemistry methods, Indoles, Kainic Acid pharmacology, MAP Kinase Kinase 4, Mitogen-Activated Protein Kinase Kinases pharmacology, Mitogen-Activated Protein Kinases metabolism, Myelin Basic Protein metabolism, Myelin Proteins metabolism, Myelin-Associated Glycoprotein metabolism, Nogo Proteins, Oligodendroglia cytology, Oligopeptides metabolism, Peroxides metabolism, Phosphorylation drug effects, Quinoxalines pharmacology, Rats, Rats, Sprague-Dawley, Reactive Oxygen Species metabolism, Receptors, Cell Surface physiology, Time Factors, Cell Death, Cerebral Cortex cytology, Excitatory Amino Acids pharmacology, JNK Mitogen-Activated Protein Kinases, Oligodendroglia drug effects, Receptors, Cell Surface metabolism, Signal Transduction drug effects
- Abstract
Oligodendroglia play an important role in axonal conduction in the CNS and are sensitive to oxidative toxicity induced by glutamate in the absence of ionotropic glutamate receptors. In this study, oligodendrocyte signalling cascades were examined, in response to glutamate-induced oxidative injury and to excitotoxicity. Rat cortical oligodendrocytes, differentiated in culture, were highly vulnerable to glutamate-induced cell death. Competitive inhibition of cystine uptake and increased oxidative stress appeared responsible for this death, and caused an accumulation of intracellular peroxides as well as chromatin fragmentation and condensation. Glutamate receptor subtype agonists (quisqualate, ibotenate) known to inhibit cystine uptake were cytotoxic, but not NMDA itself; moreover, glutamate receptor antagonists were not protective. Oligodendrocytes were also vulnerable to overactivation of glutamate receptors, as kainic acid and AMPA proved to be toxic. AMPA toxicity required the presence of cyclothiazide, suggesting rapid desensitization of AMPA receptors. Glutamate-induced oxidative stress and kainate/AMPA receptor stimulation activated the mitogen-activated protein kinase (MAP kinase) pathway, as well as the transcription factor ELK. However, MAP kinase kinase inhibitors only protected against injury from glutamate-induced oxidative stress. Oligodendrocytes were sensitive to oxygen-glucose deprivation injury as well, in a MAP kinase dependent fashion. Glutamate toxicity may conceivably be operative in neuropathological conditions that disrupt neuronal/oligodendrocyte interactions in axons, e.g. multiple sclerosis and ischaemia-reperfusion injury., (Copyright 2004 International Society for Neurochemistry)
- Published
- 2004
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26. Nitric oxide and peroxynitrite cause irreversible increases in the K(m) for oxygen of mitochondrial cytochrome oxidase: in vitro and in vivo studies.
- Author
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Cooper CE, Davies NA, Psychoulis M, Canevari L, Bates TE, Dobbie MS, Casley CS, and Sharpe MA
- Subjects
- Animals, Brain drug effects, Brain enzymology, Brain metabolism, Cattle, Cells, Cultured, Electron Transport Complex IV antagonists & inhibitors, Electron Transport Complex IV chemistry, Enzyme Activation, Enzyme Inhibitors, Gerbillinae, Mitochondria chemistry, Mitochondria drug effects, Mitochondria metabolism, Nitric Oxide chemistry, Oxygen chemistry, Peroxynitrous Acid chemistry, Electron Transport Complex IV metabolism, Mitochondria enzymology, Models, Biological, Nitric Oxide pharmacology, Oxygen metabolism, Peroxynitrous Acid pharmacology
- Abstract
Mitochondrial cytochrome oxidase is competitively and reversibly inhibited by inhibitors that bind to ferrous heme, such as carbon monoxide and nitric oxide. In the case of nitric oxide, nanomolar levels inhibit cytochrome oxidase by competing with oxygen at the enzyme's heme-copper active site. This raises the K(m) for cellular respiration into the physiological range. This effect is readily reversible and may be a physiological control mechanism. Here we show that a number of in vitro and in vivo conditions result in an irreversible increase in the oxygen K(m). These include: treatment of the purified enzyme with peroxynitrite or high (microM) levels of nitric oxide; treatment of the endothelial-derived cell line, b.End5, with NO; activation of astrocytes by cytokines; reperfusion injury in the gerbil brain. Studies of cell respiration that fail to vary the oxygen concentration systematically are therefore likely to significantly underestimate the degree of irreversible damage to cytochrome oxidase.
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- 2003
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27. Selective suppression of cathepsin L by antisense cDNA impairs human brain tumor cell invasion in vitro and promotes apoptosis.
- Author
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Levicar N, Dewey RA, Daley E, Bates TE, Davies D, Kos J, Pilkington GJ, and Lah TT
- Subjects
- Apoptosis genetics, Brain Neoplasms genetics, Brain Neoplasms pathology, Caspase 3, Caspases metabolism, Cathepsin L, Cell Adhesion drug effects, Cell Adhesion genetics, Cell Division drug effects, Cell Division genetics, Cell Movement drug effects, Cell Movement genetics, Cysteine Endopeptidases, DNA, Antisense genetics, DNA, Complementary pharmacology, Genetic Therapy methods, Glioblastoma genetics, Glioblastoma pathology, Humans, Neoplasm Invasiveness, Proto-Oncogene Proteins c-bcl-2 metabolism, Staurosporine pharmacology, Tumor Cells, Cultured, Apoptosis drug effects, Brain Neoplasms therapy, Cathepsins antagonists & inhibitors, Cathepsins genetics, DNA, Antisense pharmacology, Glioblastoma therapy
- Abstract
Invasion and metastasis of certain tumors are accompanied by increased mRNA protein levels and enzymatic activity of cathepsin L. Cathepsin L has also been suggested to play a role in the proteolytic cascades associated with apoptosis. To investigate the role of cathepsin L in brain tumor invasion and apoptosis, the human glioma cell line, IPTP, was stably transfected with full-length antisense and sense cDNA of cathepsin L. Down-regulation of cathepsin L by antisense cDNA significantly impaired (up to 70%) glioma cell invasion in vitro and markedly increased glioma cell apoptosis induced by staurosporine. Compared to control and parental cell lines, antisense down-regulation of cathepsin L was associated with an earlier induction of caspase-3 activity. Up-regulation of cathepsin L activity by sense cDNA was associated with reduced apoptosis and later induction of caspase-3 activity. Moreover, down-regulation of cathepsin L lowered the expression of antiapoptotic protein Bcl-2, whereas up-regulation increased the expression of Bcl-2, indicating that cathepsin L acts upstream of caspase-3. These data show that cathepsin L is an important protein mediating the malignancy of gliomas and its inhibition may diminish their invasion and lead to increased tumor cell apoptosis by reducing apoptotic threshold.
- Published
- 2003
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28. Delayed hypothermia prevents decreases in N-acetylaspartate and reduced glutathione in the cerebral cortex of the neonatal pig following transient hypoxia-ischaemia.
- Author
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Brooks KJ, Hargreaves I, Bhakoo K, Sellwood M, O'Brien F, Noone M, Sakata Y, Cady E, Wylezinska M, Thornton J, Ordidge R, Nguyen Q, Clemence M, Wyatt J, and Bates TE
- Subjects
- Animals, Cerebral Cortex enzymology, Citrate (si)-Synthase metabolism, Hypoxia-Ischemia, Brain enzymology, Swine, Animals, Newborn, Aspartic Acid analogs & derivatives, Aspartic Acid metabolism, Cerebral Cortex metabolism, Glutathione metabolism, Hypothermia metabolism, Hypoxia-Ischemia, Brain metabolism
- Abstract
The effects of normothermia and delayed hypothermia on the levels of N-acetylaspartate (NAA), reduced glutathione (GSH) and the activities of mitochondrial complex I, II-III, IV and citrate synthase were measured in brain homogenates obtained from anaesthetized neonatal pigs following transient in vivo hypoxia-ischaemia. In the normothermic animals there was a significant decrease in complex I activity and in the levels of GSH and NAA when compared to the controls. Delayed hypothermia preserved NAA and GSH at control levels and enhanced the rate of complex II-III activity. There was correlation (R = 0.79) between GSH and NAA levels when data from all three experimental groups were analyzed. Citrate synthase activity was not significantly different in the three groups, indicating maintenance of mitochondrial integrity. These data suggest that delayed hypothermia affords protection of integrated mitochondrial function in the neonatal brain following transient hypoxia-ischaemia.
- Published
- 2002
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29. Heterogeneity of the calcium-induced permeability transition in isolated non-synaptic brain mitochondria.
- Author
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Kristián T, Weatherby TM, Bates TE, and Fiskum G
- Subjects
- Adenosine Diphosphate chemistry, Adenosine Triphosphate chemistry, Animals, Calcium pharmacokinetics, Calcium pharmacology, Cyclosporine pharmacology, Electron Probe Microanalysis, Light, Male, Mitochondria drug effects, Mitochondria ultrastructure, Osmolar Concentration, Permeability drug effects, Potassium chemistry, Rats, Rats, Wistar, Scattering, Radiation, Succinic Acid chemistry, Brain Chemistry, Calcium chemistry, Mitochondria chemistry
- Abstract
Calcium overload of neural cell mitochondria plays a key role in excitotoxic and ischemic brain injury. This study tested the hypothesis that brain mitochondria consist of subpopulations with differential sensitivity to calcium-induced inner membrane permeability transition, and that this sensitivity is greatly reduced by physiological levels of adenine nucleotides. Isolated non-synaptosomal rat brain mitochondria were incubated in a potassium-based medium in the absence or presence of ATP or ADP. Measurements were made of medium and intramitochondrial free calcium, light scattering, mitochondrial ultrastructure, and the elemental composition of electron-opaque deposits within mitochondria treated with calcium. In the absence of adenine nucleotides, calcium induced a partial decrease in light scattering, accompanied by three distinct ultrastructural morphologies, including large-amplitude swelling, matrix vacuolization and a normal appearance. In the presence of ATP or ADP the mitochondrial calcium uptake capacity was greatly enhanced and calcium induced an increase rather than a decrease in mitochondrial light scattering. Approximately 10% of the mitochondria appeared damaged and the rest contained electron-dense precipitates that contained calcium, as determined by electron-energy loss spectroscopy. These results indicate that brain mitochondria are heterogeneous in their response to calcium. In the absence of adenine nucleotides, approximately 20% of the mitochondrial population exhibit morphological alterations consistent with activation of the permeability transition, but less than 10% exhibit evidence of osmotic swelling and membrane disruption in the presence of ATP or ADP.
- Published
- 2002
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30. Nitric oxide synthase is present in the cerebrospinal fluid of patients with active multiple sclerosis and is associated with increases in cerebrospinal fluid protein nitrotyrosine and S-nitrosothiols and with changes in glutathione levels.
- Author
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Calabrese V, Scapagnini G, Ravagna A, Bella R, Foresti R, Bates TE, Giuffrida Stella AM, and Pennisi G
- Subjects
- Adult, Blotting, Western, Catalase cerebrospinal fluid, Female, Glutathione blood, Glutathione cerebrospinal fluid, Glutathione Disulfide blood, Glutathione Disulfide cerebrospinal fluid, Humans, Male, Middle Aged, Multiple Sclerosis blood, Multiple Sclerosis enzymology, Nitrates cerebrospinal fluid, Nitric Oxide Synthase Type II, Nitrites cerebrospinal fluid, Peroxynitrous Acid cerebrospinal fluid, Recurrence, Reference Values, Cerebrospinal Fluid Proteins chemistry, Multiple Sclerosis cerebrospinal fluid, Nitric Oxide Synthase cerebrospinal fluid, S-Nitrosothiols cerebrospinal fluid, Tyrosine analogs & derivatives, Tyrosine analysis
- Abstract
Nitric oxide (NO) is hypothesized to play a role in the immunopathogenesis of multiple sclerosis (MS). Increased levels of NO metabolites have been found in patients with MS. Peroxynitrite, generated by the reaction of NO with superoxide at sites of inflammation, is a strong oxidant capable of damaging tissues and cells. Inducible NO synthase (iNOS) is up-regulated in the CNS of animals with experimental allergic encephalomyelitis (EAE) and in patients with MS. In this study, Western blots of cerebrospinal fluid (CSF) from patients with MS demonstrated the presence of iNOS, which was absent in CSF from control subjects. There was also NOS activity present in both MS and control CSF. Total NOS activity was increased (by 24%) in the CSF from MS patients compared with matched controls. The addition of 0.1 mM ITU (a specific iNOS inhibitor) to the samples did not change the activity of the control samples but decreased the NOS activity in the MS samples to almost control levels. The addition of 1 mM L-NMMA (a nonisoform specific NOS inhibitor), completely inhibited NOS activity in CSF from control and MS subjects. Nitrotyrosine immunostaining of CSF proteins was detectable in controls but was greatly increased in MS samples. There were also significant increases in CSF nitrate + nitrite and oxidant-enhanced luminescence in MS samples compared with controls. Additionally, a significant decrease in reduced glutathione and significant increases in oxidized glutathione and S-nitrosothiols were found in MS samples compared with controls. Parallel changes in NO metabolites were observed in the plasma of MS patients, compared with controls, and accompanied a significant increase of reduced glutathione. These data strongly support a role for nitrosative stress in the pathogenesis of MS and indicate that therapeutic strategies focussed on decreasing production of NO by iNOS and/or scavenging peroxynitrite may be useful in alleviating the neurological impairments that occur during MS relapse., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
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31. Mitochondrial involvement in brain function and dysfunction: relevance to aging, neurodegenerative disorders and longevity.
- Author
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Calabrese V, Scapagnini G, Giuffrida Stella AM, Bates TE, and Clark JB
- Subjects
- Animals, Humans, Aging physiology, Brain physiology, Brain physiopathology, Longevity, Mitochondria physiology, Neurodegenerative Diseases physiopathology
- Abstract
It is becoming increasingly evident that the mitochondrial genome may play a key role in neurodegenerative diseases. Mitochondrial dysfunction is characteristic of several neurodegenerative disorders, and evidence for mitochondria being a site of damage in neurodegenerative disorders is partially based on decreases in respiratory chain complex activities in Parkinson's disease, Alzheimer's disease, and Huntington's disease. Such defects in respiratory complex activities, possibly associated with oxidant/antioxidant balance perturbation, are thought to underlie defects in energy metabolism and induce cellular degeneration. Efficient functioning of maintenance and repair process seems to be crucial for both survival and physical quality of life. This is accomplished by a complex network of the so-called longevity assurance processes, which are composed of genes termed vitagenes. A promising approach for the identification of critical gerontogenic processes is represented by the hormesis-like positive effect of stress. In the present review, we discuss the role of energy thresholds in brain mitochondria and their implications in neurodegeneration. We then review the evidence for the role of oxidative stress in modulating the effects of mitochondrial DNA mutations on brain age-related disorders and also discuss new approaches for investigating the mechanisms of lifetime survival and longevity.
- Published
- 2001
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32. Heat stress contributes to the enhancement of cardiac mitochondrial complex activity.
- Author
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Sammut IA, Jayakumar J, Latif N, Rothery S, Severs NJ, Smolenski RT, Bates TE, and Yacoub MH
- Subjects
- Animals, Chaperonin 60 metabolism, Citrate (si)-Synthase metabolism, Electron Transport physiology, In Vitro Techniques, Male, Microscopy, Electron, Mitochondria, Heart metabolism, Mitochondria, Heart ultrastructure, Myocardial Ischemia physiopathology, Myocardium enzymology, Myocardium metabolism, Myocardium ultrastructure, Rats, Rats, Sprague-Dawley, Fever physiopathology, Mitochondria, Heart physiology
- Abstract
Hyperthermic stress is known to protect against myocardial dysfunction after ischemia-reperfusion injury. It is unclear however, what energetic mechanisms are affected by the molecular adaptation to heat stress. We hypothesized that mild hyperthermic stress can increase mitochondrial respiratory enzyme activity, affording protection to mitochondrial energetics during prolonged cardiac preservation for transplantation. Rat hearts were excised after heat-stress or sham treatment and subjected to cold cardioplegic arrest and ischemia followed by reperfusion in an ex vivo perfusion system. Cardiac function, mitochondrial respiratory, and complex activities were assessed before and after ischemia. Heat shock protein (Hsp 32, 60, and 72) expression was increased in heat-stressed hearts. This was associated with increased mitochondrial complex activities in heat-stress versus sham-treated groups for complex I-V. During reperfusion, higher complex activities and respiratory control ratios were observed in heat-stressed versus sham-treated groups. Recovery of ventricular function was improved in heat-stressed hearts. Furthermore, mitochondria in reperfused heat-stressed myocardium exhibited intact membranes with packed, parallel, lamellar cristae, whereas in sham-treated myocardium, mitochondria were severely disrupted. This study provides the first evidence of heat-stress-mediated enhancement of mitochondrial energetic capacity. This is associated with increased tolerance to ischemia-reperfusion injury. Protection by heat stress against myocardial dysfunction may be partially due to enhancement of mitochondrial energetics.
- Published
- 2001
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33. Effects of acetyl-L-carnitine on the formation of fatty acid ethyl esters in brain and peripheral organs after short-term ethanol administration in rat.
- Author
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Calabrese V, Scapagnini G, Catalano C, Dinotta F, Bates TE, Calvani M, and Stella AM
- Subjects
- Acyltransferases metabolism, Animals, Glutathione Transferase metabolism, Lipid Peroxides metabolism, Male, Rats, Rats, Wistar, Time Factors, Tissue Distribution, Acetylcarnitine pharmacology, Brain drug effects, Brain metabolism, Ethanol pharmacology, Fatty Acids biosynthesis
- Abstract
Increasing evidence suggests that Fatty acid ethyl esters (FAEE) play a central role in ethanol induced organ damage. In the current study we measured FAEE formation in rats after short-term oral administration of ethanol, in the presence and absence of pre-treatment with acetyl-L-carnitine. Ethanol treatment caused a significant increase in the levels of FAEE, particularly in the brain and heart, but also in the kidney and liver. Increases in FAEE were associated with a significant increase in FAEE synthase activity, GSH transferase activity, and lipid hydroperoxide levels. Pretreatment with acetyl-L-carnitine resulted in a significant reduction of FAEE accumulation, decrease in FAEE synthase and GSH transferase activities, and lipid hydroperoxide levels. Administration of acetyl-L-carnitine greatly reduced the metabolic abnormalities due to non-oxidative ethanol metabolism, through an increment in lipid metabolism/turnover and by the modulation of the activities of enzymes associated with FAEE synthesis. These results suggest a potentially important pharmacological role for acetyl-L-carnitine in the prevention of alcohol-induced cellular damage.
- Published
- 2001
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34. Activation of murine microglial cell lines by lipopolysaccharide and interferon-gamma causes NO-mediated decreases in mitochondrial and cellular function.
- Author
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Moss DW and Bates TE
- Subjects
- Adenosine Triphosphate metabolism, Animals, Cell Line, Chemokine CCL4, Energy Metabolism drug effects, Energy Metabolism physiology, Enzyme Activation drug effects, Enzyme Inhibitors pharmacology, Glutathione metabolism, Macrophage Inflammatory Proteins metabolism, Macrophages cytology, Macrophages enzymology, Mice, Microglia cytology, Nerve Degeneration metabolism, Nitric Oxide Synthase metabolism, Nitric Oxide Synthase Type II, Oxidative Stress drug effects, Oxidative Stress physiology, omega-N-Methylarginine pharmacology, Antineoplastic Agents pharmacology, Interferon-gamma pharmacology, Lipopolysaccharides pharmacology, Microglia enzymology, Mitochondria metabolism, Nitric Oxide metabolism
- Abstract
Activation of murine microglial and macrophage cell lines with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) resulted in the induction of the inducible form of nitric oxide synthase (NOS) and the release of micromolar amounts of NO into the surrounding medium. The synthesis of NO was associated with increased cellular membrane damage as assessed by trypan blue dye exclusion and the leakage of lactate dehydrogenase into the cell culture medium. However, the synthesis and release of cytokines was largely unaffected. NO-mediated cell damage was also accompanied by a marked decrease in the intracellular levels of reduced glutathione and ATP. In addition, significant inhibition of mitochondrial respiratory chain enzyme activities was seen following cellular activation. However, citrate synthase activity (a mitochondrial matrix enzyme) was not detectable in the extracellular supernatants, suggesting preservation of the integrity of the mitochondrial inner membrane following activation. These effects were largely prevented by the addition of the NOS inhibitor, N-guanidino monomethyl L-arginine during the activation period. Our observations demonstrate that induction of NOS activity in microglia results in damage to the plasma membrane leading to a loss of glutathione, complex-specific inhibition of the mitochondrial electron transport chain and depletion of cellular ATP. Our data suggest that pharmacological modulation of NOS activity in activated microglia in vivo may prevent cellular damage to bystander cells such as neurons, astrocytes and oligodendrocytes, as well as to microglia themselves.
- Published
- 2001
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35. Regulation of heat shock protein synthesis in human skin fibroblasts in response to oxidative stress: role of vitamin E.
- Author
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Calabrese V, Scapagnini G, Catalano C, Bates TE, Geraci D, Pennisi G, and Giuffrida Stella AM
- Subjects
- Cells, Cultured, Fibroblasts drug effects, Free Radicals metabolism, HSP70 Heat-Shock Proteins biosynthesis, Humans, Hydrogen Peroxide pharmacology, Oxidants pharmacology, Sulfhydryl Compounds metabolism, Vitamin E pharmacology, Fibroblasts metabolism, Heat-Shock Proteins biosynthesis, Oxidative Stress, Skin cytology, Vitamin E physiology
- Abstract
Skin plays an important role in protection against oxidative stressors such as ultraviolet radiation, ozone and chemicals. Chronic sun exposure causes degenerative changes in the skin that are recognized as photoaging. Oxidative stress has been shown to alter the expression of mammalian antioxidant enzymes as well as to enhance numerous transcription factors, including nuclear factor kappaB, stress-activated protein kinase and heat shock factor This latter is the transcription factor for the synthesis of heat shock proteins, which have been known to protect against a wide variety of toxic conditions, including extreme temperatures, oxidative stress and cytotoxic drugs. In this study we investigated the role of oxidative stress in the induction of heat shock protein (HSP) 70 in human skin fibroblasts and the effect of vitamin E. We found that significant HSP70 induction occurred after exposure to HOOH and that this was associated with a significant perturbation in protein and nonprotein sulfhydryl groups, and with a significant increase in protein carbonyl levels. Treatment with vitamin E conferred significant protection against stress-induced modifications of cellular sulfhydryl and carbonyl content, while maintaining functional levels of cytoprotective HSP70. Our results point to the possible involvement of redox mechanisms in the heat shock signal transduction pathway, which may play an important regulatory role in the genetic mechanisms of tolerance to oxidative stress. Exogenous antioxidant supplementation with vitamin E could have cosmetic benefits and may be an efficient tool to mitigate the consequences of free radical-induced skin damage.
- Published
- 2001
36. Induction of heat shock protein synthesis in human skin fibroblasts in response to oxidative stress: regulation by a natural antioxidant from rosemary extract.
- Author
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Calabrese V, Scapagnini G, Catalano C, Bates TE, Dinotta F, Micali G, and Giuffrida Stella AM
- Subjects
- Blotting, Western, Cells, Cultured, Fibroblasts metabolism, Hot Temperature adverse effects, Humans, Oxidation-Reduction, Rosmarinus, Signal Transduction, Time Factors, Antioxidants pharmacology, Fibroblasts drug effects, HSP70 Heat-Shock Proteins biosynthesis, Hydrogen Peroxide pharmacology, Oxidative Stress physiology, Plant Extracts pharmacology, Skin metabolism, Sulfhydryl Compounds metabolism
- Abstract
Reactive oxygen species have been implicated in the pathogenesis of the severe connective tissue damage present in several photodermatologic disorders, including drug-induced phototoxicity, porphyrias and photoaging. Oxidative stress has been shown to alter the expression of mammalian antioxidant enzymes and to enhance numerous transcription factors, including nuclear factor-kappa B, stress-activated protein kinase and heat shock factor. The latter represents the transcription factor for the synthesis of cytoprotective proteins called heat shock proteins. In this study, we investigated the role of oxidative stress and sulfdryl (SH) groups in the induction of HSP70 in human skin fibroblasts and the effect of antioxidants. We found that significant HSP70 induction occurred after exposure to HOOH and this was associated with marked perturbation in protein and nonprotein SH groups and with a considerable increase in protein carbonyl levels. Treatment with a natural antioxidant from rosemary extract provided notable protection against stress-induced modifications of cellular SH and carbonyl content, maintaining functional levels of cytoprotective heat shock protein 70. Our results point to the possible involvement of redox mechanisms in the heat shock signal transduction pathway, which may play an important regulatory role in the genetic mechanisms of tolerance to oxidative stress. Exogenous supplementation of an antioxidant hydrophilic extract from rosemary could have cosmetic benefits and may represent an efficient tool to minimize free radical-induced skin damage.
- Published
- 2001
37. NO synthase and NO-dependent signal pathways in brain aging and neurodegenerative disorders: the role of oxidant/antioxidant balance.
- Author
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Calabrese V, Bates TE, and Stella AM
- Subjects
- Aging, Animals, Brain growth & development, Brain physiopathology, Heat-Shock Proteins physiology, Humans, Neurodegenerative Diseases enzymology, Signal Transduction physiology, Antioxidants metabolism, Brain physiology, Neurodegenerative Diseases physiopathology, Nitric Oxide physiology, Nitric Oxide Synthase metabolism, Oxidants metabolism
- Abstract
Nitric oxide and other reactive nitrogen species appear to play several crucial roles in the brain. These include physiological processes such as neuromodulation, neurotransmission and synaptic plasticity, and pathological processes such as neurodegeneration and neuroinflammation. There is increasing evidence that glial cells in the central nervous system can produce nitric oxide in vivo in response to stimulation by cytokines and that this production is mediated by the inducible isoform of nitric oxide synthase. Although the etiology and pathogenesis of the major neurodegenerative and neuroinflammatory disorders (Alzheimer's disease, amyothrophic lateral sclerosis, Parkinson's disease, Huntington's disease and multiple sclerosis) are unknown, numerous recent studies strongly suggest that reactive nitrogen species play an important role. Furthermore, these species are probably involved in brain damage following ischemia and reperfusion, Down's syndrome and mitochondrial encephalopathies. Recent evidence also indicates the importance of cytoprotective proteins such as heat shock proteins (HSPs) which appear to be critically involved in protection from nitrosative and oxidative stress. In this review, evidence for the involvement of nitrosative stress in the pathogenesis of the major neurodegenerative/ neuroinflammatory diseases and the mechanisms operating in brain as a response to imbalance in the oxidant/antioxidant status are discussed.
- Published
- 2000
- Full Text
- View/download PDF
38. Nitric-oxide-induced inhibition of mitochondrial complexes following aglycaemic hypoxia in neonatal cortical rat brain slices.
- Author
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Brooks KJ, Hargreaves IP, and Bates TE
- Subjects
- Animals, Animals, Newborn, Cerebral Cortex cytology, Cerebral Cortex drug effects, Citrate (si)-Synthase metabolism, Electron Transport Complex I, Electron Transport Complex II, Electron Transport Complex III metabolism, Electron Transport Complex IV metabolism, Female, Glucose deficiency, In Vitro Techniques, Male, Mitochondria drug effects, Multienzyme Complexes metabolism, NADH, NADPH Oxidoreductases metabolism, Nitric Oxide pharmacology, Oxidoreductases metabolism, Rats, Rats, Wistar, Succinate Dehydrogenase metabolism, Cerebral Cortex metabolism, Glucose metabolism, Hypoxia, Brain metabolism, Mitochondria enzymology, Nitric Oxide metabolism
- Abstract
The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused neonatal cortical brain slices. After 30 min AH, there were no significant changes in the activities of complex I, II-III and IV or citrate synthase compared to controls. Following 30 min AH and a 30-min reperfusion period (with oxygen and glucose), the activities of complex II-III and complex IV were significantly reduced (by 25 and 17%, respectively). These reductions in enzyme activity were not abrogated by removing external calcium prior to and throughout AH, but could be reversed by the presence of the nitric oxide (NO) synthase inhibitor N omega-nitro-L-arginine during these periods. These data suggest that NO or an NO-derived species is involved in the decreases in mitochondrial enzyme activities observed after AH, (Copyright 2000 S. Karger AG, Basel)
- Published
- 2000
- Full Text
- View/download PDF
39. Characteristics of the calcium-triggered mitochondrial permeability transition in nonsynaptic brain mitochondria: effect of cyclosporin A and ubiquinone O.
- Author
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Kristián T, Gertsch J, Bates TE, and Siesjö BK
- Subjects
- Animals, Brain ultrastructure, Calcium metabolism, Calcium pharmacology, Cyclosporine pharmacology, Male, Mitochondria metabolism, Mitochondria ultrastructure, Mitochondria, Heart metabolism, Mitochondria, Muscle metabolism, Permeability drug effects, Rats, Rats, Wistar, Ubiquinone pharmacology, Brain metabolism, Calcium physiology
- Abstract
The objective of the present study was to assess the capacity of nonsynaptic brain mitochondria to accumulate Ca2+ when subjected to repeated Ca2+ loads, and to explore under what conditions a mitochondrial permeability transition (MPT) pore is assembled. The effects of cyclosporin A (CsA) on Ca2+ accumulation and MPT pore assembly were compared with those obtained with ubiquinone 0 (Ubo), a quinone that is a stronger MPT blocker than CsA, when tested on muscle and liver mitochondria. When suspended in a solution containing phosphate (2 mM) and Mg2+ (1 mM), but no ATP or ADP, the brain mitochondria had a limited capacity to accumulate Ca2+ (210 nmol/mg of mitochondrial protein). Furthermore, when repeated Ca2+ pulses (40 nmol/mg of protein each) saturated the uptake system, the mitochondria failed to release the Ca2+ accumulated. However, in each instance, the first Ca2+ pulse was accompanied by a moderate release of Ca2+, a release that was not observed during the subsequent pulses. The initial release was accompanied by a relatively marked depolarization, and by swelling, as assessed by light-scattering measurements. However, as the swelling was <50% of that observed following addition of alamethicin, it is concluded that the first Ca2+ pulse gives rise to an MPT in a subfraction of the mitochondrial population. CsA, an avid blocker of the MPT pore, only marginally increased the Ca(2+)-sequestrating capacity of the mitochondria. However, CsA eliminated the Ca2+ release accompanying the first Ca2+ pulse. The effects of CsA were shared by Ubo, but when the concentration of Ubo exceeded 20 microM, it proved toxic. The results thus suggest that brain mitochondria are different from those derived from a variety of other sources. The major difference is that a fraction of the brain mitochondria, studied presently, depolarized and showed signs of an MPT. This fraction, but not the remaining ones, contributed to the chemically and electron microscopically verified mitochondrial swelling.
- Published
- 2000
- Full Text
- View/download PDF
40. Time-dependent impairment of mitochondrial function after storage and transplantation of rabbit kidneys.
- Author
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Sammut IA, Burton K, Balogun E, Sarathchandra P, Brooks KJ, Bates TE, and Green CJ
- Subjects
- Animals, Citrate (si)-Synthase metabolism, Electron Transport physiology, Enzymes metabolism, Kidney ultrastructure, Male, Mitochondria enzymology, Mitochondria metabolism, Oxygen Consumption, Postoperative Period, Rabbits, Time Factors, Cryopreservation, Kidney physiology, Kidney Transplantation, Mitochondria physiology
- Abstract
Background: The mitochondrial respiratory chain is implicated as a major target of kidney damage after ischemia-reperfusion. This study measures changes in integrated mitochondrial function and in the activity of enzymes of the respiratory chain after cold storage and transplantation-reperfusion in vivo., Methods: Mitochondrial oxygen consumption and activities of respiratory chain enzymes and citrate synthase were measured in cortical mitochondria isolated from rabbit kidneys after 1-48 hr of cold ischemia with or without transplantation-reperfusion., Results: State 4 mitochondrial oxygen consumption was significantly increased after 48 hr of ischemia or 24-48 hr of ischemia with transplantation. Prolonged (24 or 48 hr) ischemic storage with and without transplantation caused a significant decrease in state 3 oxygen consumption, as did transplantation after 1, 24, and 48 hr of cold storage. Complex I and complex II-III activity decreased after 24 or 48 hr of ischemia, with transplantation having little additional effect. Complex IV activity was significantly decreased after 48 hr of ischemia, this decrease being exacerbated by transplantation-reperfusion. Complex V activity decreased significantly after 1 hr of ischemia and continued to decrease after 24-48 hr of ischemia. Transplantation after 1-24 hr (but not 48 hr) of ischemia resulted in partial recovery of complex V activity. Citrate synthase activity was decreased significantly only after 48 hr of ischemia and reperfusion, consistent with the loss of mitochondrial membrane integrity seen in electron micrographs of the transplanted 48-hr group., Conclusions: These data suggest that individual rabbit kidney mitochondrial complexes have different susceptibilities to cold ischemic and reperfusion damage.
- Published
- 2000
- Full Text
- View/download PDF
41. HSP70 induction in the brain following ethanol administration in the rat: regulation by glutathione redox state.
- Author
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Calabrese V, Testa G, Ravagna A, Bates TE, and Stella AM
- Subjects
- Animals, Brain metabolism, Male, Oxidation-Reduction, Rats, Rats, Wistar, Brain drug effects, Ethanol pharmacology, Glutathione metabolism, HSP70 Heat-Shock Proteins biosynthesis
- Abstract
Changes in glutathione (GSH) and glutathione disulfide (GSSG) levels and/or redox status have been suggested to mediate the induction of heat shock proteins (HSPs) that follows exposure to oxidizing agents such as ethanol. Here we report the effects of ethanol administration to rats at intracellular levels of GSH, GSSG, HSP70, and protein carbonyls in brain and liver. Following 7 days of ethanol administration, there was a significant decrease in GSH, a significant induction of HSP70, and a significant increase in protein carbonyls in all brain regions studied and in liver. In cortex, striatum, and hippocampus there was a significant correlation between (a) the decrease in GSH, (b) the increase in GSSG, and (c) the decrease in GSH/GSSG ratio and HSP70 levels induced in response to ethanol. These data support the hypothesis that a redox mechanism may be involved in the heat-shock signal pathway responsible for HSP70 induction in the brain., (Copyright 2000 Academic Press.)
- Published
- 2000
- Full Text
- View/download PDF
42. beta-Amyloid fragment 25-35 selectively decreases complex IV activity in isolated mitochondria.
- Author
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Canevari L, Clark JB, and Bates TE
- Subjects
- Animals, Brain metabolism, Citrate (si)-Synthase metabolism, Electron Transport physiology, Electron Transport Complex IV antagonists & inhibitors, Kinetics, Male, Neurodegenerative Diseases metabolism, Rats, Rats, Wistar, Time Factors, Amyloid beta-Peptides metabolism, Amyloid beta-Peptides physiology, Electron Transport Complex IV metabolism, Mitochondria metabolism
- Abstract
Defects in mitochondrial oxidative metabolism, in particular decreased activity of cytochrome c oxidase, have been demonstrated in Alzheimer's disease, and after the expression of the amyloid precursor protein (APP) in cultured cells, suggesting that mitochondria might be involved in beta-amyloid toxicity. Recent evidence suggests that the proteolysis of APP to generate beta-amyloid is at least in part intracellular, preceding the deposition of extracellular fibrils. We have therefore investigated the effect of incubation of isolated rat brain mitochondria with the beta-amyloid fragment 25-35 (100 microM) on the activities of the mitochondrial respiratory chain complexes I, II-III, IV (cytochrome c oxidase) and citrate synthase. The peptide caused a rapid, dose-dependent decrease in the activity of complex IV, white it had no effect on the activities on any of the other enzymes tested. The reverse sequence peptide (35-25) had no effect on any of the activities measured. We conclude that inhibition of mitochondrial complex IV might be a contributing factor to the pathogenesis of Alzheimer's disease.
- Published
- 1999
- Full Text
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43. Effect of hydrazine upon vitamin B12-dependent methionine synthase activity and the sulphur amino acid pathway in isolated rat hepatocytes.
- Author
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Kenyon SH, Waterfield CJ, Asker DS, Kudo M, Moss DW, Bates TE, Nicolaou A, Gibbons WA, and Timbrell JA
- Subjects
- Animals, Carcinogens pharmacology, Cell Survival drug effects, Culture Media metabolism, Cysteine metabolism, Cytosol metabolism, Enzyme Inhibitors pharmacology, Homocysteine metabolism, Liver, Male, Nitroarginine pharmacology, Rats, Rats, Wistar, Taurine metabolism, 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase metabolism, Amino Acids, Sulfur metabolism, Hydrazines pharmacology, Vitamin B 12 metabolism
- Abstract
The effect of the industrial chemical, hydrazine (4-12 mM), on methionine synthase (EC 2.1.1.13) activity and levels of the sulphur amino acids homocysteine, cysteine, and taurine as well as GSH were investigated in vitro in isolated rat hepatocyte suspensions and monolayers in order to explain some of the adverse in vivo effects of hydrazine. None of the concentrations of hydrazine were overtly cytotoxic in hepatocyte suspensions (measured as lactate dehydrogenase [LDH] leakage) after 3 hr. However, after 24 hr in culture cells treated with 12 mM, hydrazine showed a significant increase in LDH leakage. Methionine synthase activity was reduced by hydrazine (8 and 12 mM) in suspensions (by 45 and 55%, after 3 hr) and monolayers (12 mM; 65-80% after 24 hr). This was not due to nitric oxide production and the inhibitor of nitric oxide synthase, Nomega-nitro-L-arginine, failed to protect against the hydrazine-induced loss of ATP and GSH and the reduction in urea synthesis at 24 hr. Homocysteine export was increased by 6 mM hydrazine, and total taurine content of treated cells was increased by 12 mM hydrazine. Thus, hydrazine was found to have several important and possibly deleterious effects on some parts of the sulphur amino acid pathway.
- Published
- 1999
- Full Text
- View/download PDF
44. Heightened resistance of the neonatal brain to ischemia-reperfusion involves a lack of mitochondrial damage in the nerve terminal.
- Author
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Keelan J, Bates TE, and Clark JB
- Subjects
- Analysis of Variance, Animals, Animals, Newborn, Brain enzymology, Female, Male, Mitochondria enzymology, Rats, Rats, Wistar, Reactive Oxygen Species metabolism, Reperfusion Injury enzymology, Synaptosomes enzymology, Brain ultrastructure, Mitochondria ultrastructure, Reperfusion Injury pathology, Synaptosomes ultrastructure
- Abstract
Mitochondria are known targets of ischemia-reperfusion injury in adult brain. Although neonates are more resistant to ischemic episodes, the mechanisms accounting for this are not yet fully understood. The aim of this study therefore was to determine whether a difference in post-ischemic mitochondrial function may play a role in the heightened recovery of the neonatal brain following ischemia-reperfusion. We have therefore compared the effects of an in vitro model of ischemia on the enzymes of the mitochondrial respiratory chain in isolated nerve terminals (synaptosomes) from neonatal and adult rats. Ischemia caused a significant, reversible decrease in mitochondrial Complex I activity in both adult and neonatal preparations. In neonatal preparations alone, ischemia also led to a significant decrease in mitochondrial Complexes II-III activity. Following 30 min of reperfusion mitochondrial Complexes II-III and IV exhibited decreased activity in synaptosomes from adult, but not neonatal rats. These data suggest a difference in the susceptibility of adult as compared to neonatal nerve terminal mitochondria to ischemia-reperfusion. These data show for the first time that nerve terminal mitochondria from immature animals remain undamaged following a period of ischemia and reperfusion, in contrast to nerve terminal mitochondria from the adult brain. This adds to the growing body of evidence that mitochondrial function plays a key role in neuronal death following cerebral ischemia reperfusion., (Copyright 1999 Elsevier Science B.V.)
- Published
- 1999
- Full Text
- View/download PDF
45. Effect of postischaemic hypothermia on the mitochondrial damage induced by ischaemia and reperfusion in the gerbil.
- Author
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Canevari L, Console A, Tendi EA, Clark JB, and Bates TE
- Subjects
- Analysis of Variance, Animals, Brain Ischemia pathology, Gerbillinae, Oxygen Consumption physiology, Synaptosomes pathology, Brain Ischemia therapy, Hypothermia, Induced, Mitochondria pathology, Reperfusion Injury pathology
- Abstract
In order to test the effect of hypothermia on mitochondrial function damage following cerebral ischaemia/reperfusion, Mongolian gerbils were submitted to 30 min bilateral carotid occlusion and 2 h of reperfusion at 37 degreesC or 30 degreesC. After normothermic (37 degreesC) ischaemia/reperfusion, significant decreases in mitochondrial state 3 (+ADP) oxygen consumption (-42.2%), complex II-III activity in synaptosomes (-31.7%) and complex IV were measured, in both free mitochondria and synaptosomes (-30.3% and -27. 8% respectively). However, following hypothermic (30 degreesC) reperfusion, both respiration rates and all enzyme activities remained at levels not significantly different from those in the sham operated controls., (Copyright 1999 Elsevier Science B.V.)
- Published
- 1999
- Full Text
- View/download PDF
46. Ischaemia-induced damage to mitochondrial complexes in the neonatal brain--role of NO.
- Author
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Brooks KJ, Hargreaves IP, Clark JB, and Bates TE
- Subjects
- Animals, Animals, Newborn, Cerebral Cortex drug effects, Cerebral Cortex enzymology, Cerebral Cortex metabolism, Enzyme Inhibitors pharmacology, Glucose metabolism, In Vitro Techniques, NG-Nitroarginine Methyl Ester pharmacology, Nitric Oxide Synthase antagonists & inhibitors, Nitroarginine pharmacology, Oxygen metabolism, Rats, Brain Ischemia metabolism, Mitochondria metabolism, Nitric Oxide metabolism
- Published
- 1998
- Full Text
- View/download PDF
47. 3-Hydroxybutyrate aids the recovery of the energy state from aglycaemic hypoxia of adult but not neonatal rat brain slices.
- Author
-
Brooks KJ, Clark JB, and Bates TE
- Subjects
- 3-Hydroxybutyric Acid, Animals, Female, Hypoxia blood, In Vitro Techniques, Magnetic Resonance Spectroscopy, Male, Phosphorus, Rats, Rats, Wistar, Animals, Newborn metabolism, Blood Glucose metabolism, Brain metabolism, Energy Metabolism drug effects, Hydroxybutyrates pharmacology, Hypoxia metabolism
- Abstract
The level of phosphocreatine (PCr) and the intracellular pH (pHi) of superfused cortical brain slices from adult or 10-day-old rats were monitored using 31P NMR. When the glucose in the superfusing medium was replaced by 3-hydroxybutyrate (3HB), there was a significant reduction in PCr of the adult but not the neonatal slices. The level of PCr of the adult slices was reduced by a greater amount by aglycaemic hypoxia compared with the neonatal brain slices and pHi was decreased by the same amount. After aglycaemic hypoxia, the levels of PCr of the neonatal slices recovered to the same extent when perfused with glucose or 3HB alone or a mixture of glucose and 3HB. The recovery of the PCr was significantly more in the neonatal than the adult brain slices with glucose alone after aglycaemic hypoxia, whereas pHi returned to control levels in both tissue types and with all substrates. The relative recovery of the PCr of the adult slices after aglycaemic hypoxia was the same with either 3HB or glucose. However, if glucose and 3HB were applied together, recovery of PCr was significantly improved compared with glucose alone.
- Published
- 1998
- Full Text
- View/download PDF
48. Impairment of hepatic mitochondrial respiratory function following storage and orthotopic transplantation of rat livers.
- Author
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Sammut IA, Thorniley MS, Simpkin S, Fuller BJ, Bates TE, and Green CJ
- Subjects
- Adenosine Triphosphate metabolism, Animals, Electron Transport, Flavin-Adenine Dinucleotide metabolism, In Vitro Techniques, Liver blood supply, Liver injuries, Male, NAD metabolism, Oxidative Phosphorylation, Oxygen Consumption, Rats, Rats, Inbred Lew, Reperfusion Injury etiology, Reperfusion Injury metabolism, Reperfusion Injury prevention & control, Cryopreservation, Liver Transplantation physiology, Mitochondria, Liver metabolism, Organ Preservation adverse effects
- Abstract
Prolonged storage of organs for transplant results in tissue damage which may be compounded on reperfusion of the graft tissue. The effect of storage times was examined on hepatic mitochondrial oxygen consumption and activities of complexes I, II-III, IV, and V in mitochondria isolated from rat liver isografts stored for 25 min and 24 h pre- and posttransplantation. While Complex I activity was significantly (P < 0.05) inhibited under all the conditions studied, Complex II-III activity was only significantly (P < 0.05) reduced following transplantation of 24-h stored tissue. Complex IV activity remained unchanged under all the conditions studied. Although Complex V activity was significantly damaged within the first 25 min of ischemia, activity values were partially recovered to control levels following 3 h of reperfusion after transplantation. Prolonged (24 h) storage induced decreases in Complex V activity which were irrecoverable. Mitochondria subjected to 25 min ischemia alone also showed a significant (P < 0.01) decrease in NAD(+)-linked respiratory control indices due to a stimulated state 4 rate. The 24-h storage and transplantation brought about a significantly (P < 0.001) greater inhibition of respiratory control and state 3 respiration. FAD-linked respiration parameters were significantly (P < 0.05) affected in livers subjected to prolonged (24 h) storage or transplantation. These data suggest that a loss of membrane integrity coupled with an inhibition of Complexes I and V and an involvement of Complex II-III in 24-h stored hepatic transplants accounts for mitochondrial respiratory dysfunction in hepatic transplantation injury. No indication of Complex IV damage was found in this study. This study shows that damage to specific mitochondrial complexes occurs as a consequence of hypothermic ischemic injury.
- Published
- 1998
- Full Text
- View/download PDF
49. Activity of mitochondrial respiratory chain enzymes after transient focal ischemia in the rat.
- Author
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Canevari L, Kuroda S, Bates TE, Clark JB, and Siesjö BK
- Subjects
- Animals, Cyclic N-Oxides, Male, Nitrogen Oxides pharmacology, Rats, Rats, Wistar, Electron Transport Complex IV metabolism, Enzymes metabolism, Ischemic Attack, Transient metabolism, Mitochondria metabolism, Oxygen Consumption physiology
- Abstract
Previous results demonstrated that after 2-hour middle cerebral artery occlusion (MCAO) in the rat, 1- to 2-hour recirculation temporarily restored the bioenergetic state and mitochondrial function, but secondary deterioration took place after 4 hours. The authors measured the activity of mitochondrial respiratory chain complexes, citrate synthase, and glutamate dehydrogenase as possible targets of secondary damage. Focal and penumbral tissues were sampled in the control condition, after 2 hours of MCAO, and after 1, 2, or 4 hours of postischemic recirculation; two groups were treated with alpha-phenyl-N-tert-butyl-nitrone (PBN). Complex IV activity transiently decreased after MCAO, but after recirculation all measured activities returned to control values.
- Published
- 1997
- Full Text
- View/download PDF
50. [U-13C]glutamate metabolism in rat brain mitochondria reveals malic enzyme activity.
- Author
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Bakken IJ, Sonnewald U, Clark JB, and Bates TE
- Subjects
- Animals, Brain enzymology, Carbon Isotopes, In Vitro Techniques, Magnetic Resonance Spectroscopy, Male, Mitochondria enzymology, Pyruvates metabolism, Rats, Rats, Wistar, Brain metabolism, Glutamic Acid metabolism, Malate Dehydrogenase metabolism, Malates metabolism, Mitochondria metabolism
- Abstract
13C nuclear magnetic resonance spectroscopy was used to study the activity of malic enzyme in isolated brain mitochondria from rat in the presence of unlabelled malate and [U-13C]glutamate. ADP, inorganic phosphate, malate and [U-13C]glutamate were added to a suspension of oxygenated mitochondria. Typical tricarboxylic acid (TCA) cycle constituents (malate, 2-oxoglutarate and succinate) were labelled from [U-13C]glutamate and detected in the superfusion medium. The labelling patterns in the different atom positions of glutamate revealed entry of both unlabelled and labelled acetyl-CoA into the TCA cycle. Unlabelled acetyl-CoA was derived via pyruvate from exogenously applied malate by the action of mitochondrial malic enzyme, while labelled acetyl-CoA was derived from TCA cycle intermediates, most likely by the action of mitochondrial malic enzyme on malate produced from [U-13C]glutamate. The results demonstrate malic enzyme activity and pyruvate recycling in isolated rat brain mitochondria.
- Published
- 1997
- Full Text
- View/download PDF
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