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Nitric-oxide-induced inhibition of mitochondrial complexes following aglycaemic hypoxia in neonatal cortical rat brain slices.
- Source :
-
Developmental neuroscience [Dev Neurosci] 2000 Sep-Dec; Vol. 22 (5-6), pp. 359-65. - Publication Year :
- 2000
-
Abstract
- The effect of aglycaemic hypoxia (AH) on the activity of the mitochondrial respiratory chain complexes was measured in superfused neonatal cortical brain slices. After 30 min AH, there were no significant changes in the activities of complex I, II-III and IV or citrate synthase compared to controls. Following 30 min AH and a 30-min reperfusion period (with oxygen and glucose), the activities of complex II-III and complex IV were significantly reduced (by 25 and 17%, respectively). These reductions in enzyme activity were not abrogated by removing external calcium prior to and throughout AH, but could be reversed by the presence of the nitric oxide (NO) synthase inhibitor N omega-nitro-L-arginine during these periods. These data suggest that NO or an NO-derived species is involved in the decreases in mitochondrial enzyme activities observed after AH<br /> (Copyright 2000 S. Karger AG, Basel)
- Subjects :
- Animals
Animals, Newborn
Cerebral Cortex cytology
Cerebral Cortex drug effects
Citrate (si)-Synthase metabolism
Electron Transport Complex I
Electron Transport Complex II
Electron Transport Complex III metabolism
Electron Transport Complex IV metabolism
Female
Glucose deficiency
In Vitro Techniques
Male
Mitochondria drug effects
Multienzyme Complexes metabolism
NADH, NADPH Oxidoreductases metabolism
Nitric Oxide pharmacology
Oxidoreductases metabolism
Rats
Rats, Wistar
Succinate Dehydrogenase metabolism
Cerebral Cortex metabolism
Glucose metabolism
Hypoxia, Brain metabolism
Mitochondria enzymology
Nitric Oxide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0378-5866
- Volume :
- 22
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Developmental neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 11111151
- Full Text :
- https://doi.org/10.1159/000017461