1. Lambertellin from Pycnoporus sanguineus MUCL 51321 and its anti-inflammatory effect via modulation of MAPK and NF-κB signaling pathways.
- Author
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Jouda JB, Mfotie Njoya E, Mbazoa CD, Zhou Z, Meli Lannang A, Wandji J, Shiono Y, and Wang F
- Subjects
- Animals, Anti-Inflammatory Agents isolation & purification, Anti-Inflammatory Agents pharmacology, Cyclooxygenase 2 genetics, Cyclooxygenase 2 metabolism, Down-Regulation drug effects, Lipopolysaccharides pharmacology, Macrophages cytology, Macrophages drug effects, Macrophages metabolism, Mice, Naphthalenes isolation & purification, Naphthalenes pharmacology, Nitric Oxide metabolism, Nitric Oxide Synthase Type II genetics, Nitric Oxide Synthase Type II metabolism, Pycnoporus metabolism, RAW 264.7 Cells, Signal Transduction drug effects, Spiro Compounds isolation & purification, Spiro Compounds pharmacology, Anti-Inflammatory Agents chemistry, Mitogen-Activated Protein Kinases metabolism, NF-kappa B metabolism, Naphthalenes chemistry, Pycnoporus chemistry, Spiro Compounds chemistry
- Abstract
Lambertellin (1) and ergosta-5,7,22-trien-3-ol (2) were isolated from the solid rice fermentation of the plant pathogenic fungus Pycnoporus sanguineus MUCL 51321. Their structures were elucidated using comprehensive spectroscopic methods. The isolated compounds were tested on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Lambertellin (1) exhibited promising inhibitory activity against nitric oxide (NO) production with IC
50 value of 3.19 µM, and it significantly inhibited the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). Lambertellin (1) also decreased the expression of pro-inflammatory cytokines IL-6 and IL-1β. The study of the mechanistic pathways revealed that lambertellin (1) exerts its anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophage cells by modulating the activation of the mitogen activated protein kinase (MAPK) and nuclear factor κB (NF-κB) signaling pathways. Therefore, lambertellin (1) could be a promising lead compound for the development of new anti-inflammatory drugs., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2018
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