11 results on '"Falkard, Brie"'
Search Results
2. Inhibition of HIV-1 Replication by elF3f
- Author
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Valente, Susana T., Gilmartin, Greg M., Mott, Christina, Falkard, Brie, and Goff, Stephen P.
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- 2009
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3. Validation of Candidate Causal Genes for Abdominal Obesity Which Affect Shared Metabolic Pathways and Networks
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Yang, Xia, Deignan, Joshua L., Qi, Hongxiu, Zhu, Jun, Qian, Su, Zhong, Judy, Torosyan, Gevork, Majid, Sana, Falkard, Brie, Kleinhanz, Robert R., Karlsson, Jenny, Castellani, Lawrence W., Mumick, Sheena, Wang, Kai, Xie, Tao, Coon, Michael, Zhang, Chunsheng, Estrada-Smith, Daria, Farber, Charles R., Wang, Susanna S., Van Nas, Atila, Ghazalpour, Anatole, Zhang, Bin, MacNeil, Douglas J., Lamb, John R., Dipple, Katrina M., Reitman, Marc L., Mehrabian, Margarete, Lum, Pek Y., Schadt, Eric E., Lusis, Aldons J., and Drake, Thomas A.
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Male ,Mice, Knockout ,Glutathione Peroxidase ,Transcription, Genetic ,Gene Expression Profiling ,Vesicular Transport Proteins ,Genetic Variation ,Reproducibility of Results ,Mice, Transgenic ,Nerve Tissue Proteins ,Article ,Disease Models, Animal ,Mice ,Phenotype ,Adipose Tissue ,Liver ,Abdomen ,Animals ,Humans ,Female ,Obesity ,Carrier Proteins ,Muscle, Skeletal ,Glycoproteins - Abstract
A principal task in dissecting the genetics of complex traits is to identify causal genes for disease phenotypes. We previously developed a method to infer causal relationships among genes through the integration of DNA variation, gene transcription and phenotypic information. Here we have validated our method through the characterization of transgenic and knockout mouse models of genes predicted to be causal for abdominal obesity. Perturbation of eight out of the nine genes, with Gas7, Me1 and Gpx3 being newly confirmed, resulted in significant changes in obesity-related traits. Liver expression signatures revealed alterations in common metabolic pathways and networks contributing to abdominal obesity and overlapped with a macrophage-enriched metabolic network module that is highly associated with metabolic traits in mice and humans. Integration of gene expression in the design and analysis of traditional F(2) intercross studies allows high-confidence prediction of causal genes and identification of pathways and networks involved.
- Published
- 2009
4. Bivalent oral cholera vaccination induces a memory B cell response to the V. cholerae O1-polysacchide in Haitian adults.
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Falkard, Brie, Charles, Richelle C., Matias, Wilfredo R., Mayo-Smith, Leslie M., Jerome, J. Gregory, Offord, Evan S., Xu, Peng, Kováč, Pavol, Ryan, Edward T., Qadri, Firdausi, Franke, Molly F., Ivers, Louise C., and Harris, Jason B.
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B cells , *ORAL vaccines , *ANTIBODY formation , *MEMORY , *CHOLERA vaccines , *IMMUNOGLOBULIN producing cells - Abstract
The bivalent killed whole-cell oral cholera vaccine (BivWC) is being increasingly used to prevent cholera. The presence of O-antigen-specific memory B cells (MBC) has been associated with protective immunity against cholera, yet MBC responses have not been evaluated after BivWC vaccination. To address this knowledge gap, we measured V. cholerae O1-antigen MBC responses following BivWC vaccination. Adults in St. Marc, Haiti, received 2 doses of the BivWC vaccine, Shanchol, two weeks apart. Participants were invited to return at days 7, 21, 44, 90, 180 and 360 after the initial vaccination. Serum antibody and MBC responses were assessed at each time-point before and following vaccination. We observed that vaccination with BivWC resulted in significant O-antigen specific MBC responses to both Ogawa and Inaba serotypes that were detected by day 21 and remained significantly elevated over baseline for up to 12 months following vaccination. The BivWC oral cholera vaccine induces durable MBC responses to the V. cholerae O1-antigen. This suggests that long-term protection observed following vaccination with BivWC could be mediated or maintained by MBC responses. [ABSTRACT FROM AUTHOR]
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- 2019
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5. Antibody Secreting Cell Responses following Vaccination with Bivalent Oral Cholera Vaccine among Haitian Adults.
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Matias, Wilfredo R., Falkard, Brie, Charles, Richelle C., Mayo-Smith, Leslie M., Teng, Jessica E., Xu, Peng, Kováč, Pavol, Ryan, Edward T., Qadri, Firdausi, Franke, Molly F., Ivers, Louise C., and Harris, Jason B.
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PREVENTION of cholera , *IMMUNE response , *VACCINATION , *ANTIGENS , *VIBRIO infections - Abstract
Background: The bivalent whole-cell (BivWC) oral cholera vaccine (Shanchol) is effective in preventing cholera. However, evaluations of immune responses following vaccination with BivWC have been limited. To determine whether BivWC induces significant mucosal immune responses, we measured V. cholerae O1 antigen-specific antibody secreting cell (ASC) responses following vaccination. Methodology/Principal Findings: We enrolled 24 Haitian adults in this study, and administered doses of oral BivWC vaccine 14 days apart (day 0 and day 14). We drew blood at baseline, and 7 days following each vaccine dose (day 7 and 21). Peripheral blood mononuclear cells (PBMCs) were isolated, and ASCs were enumerated using an ELISPOT assay. Significant increases in Ogawa (6.9 cells per million PBMCs) and Inaba (9.5 cells per million PBMCs) OSP-specific IgA ASCs were detected 7 days following the first dose (P < 0.001), but not the second dose. The magnitude of V. cholerae-specific ASC responses did not appear to be associated with recent exposure to cholera. ASC responses measured against the whole lipolysaccharide (LPS) antigen and the OSP moiety of LPS were equivalent, suggesting that all or nearly all of the LPS response targets the OSP moiety. Conclusions/Significance: Immunization with the BivWC oral cholera vaccine induced ASC responses among a cohort of healthy adults in Haiti after a single dose. The second dose of vaccine resulted in minimal ASC responses over baseline, suggesting that the current dosing schedule may not be optimal for boosting mucosal immune responses to V. cholerae antigens for adults in a cholera-endemic area. [ABSTRACT FROM AUTHOR]
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- 2016
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6. Parasite-induced ER stress response in hepatocytes facilitates Plasmodium liver stage infection.
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Inácio, Patricia, Zuzarte‐Luís, Vanessa, Ruivo, Margarida TG, Falkard, Brie, Nagaraj, Nagarjuna, Rooijers, Koos, Mann, Matthias, Mair, Gunnar, Fidock, David A, and Mota, Maria M
- Abstract
Upon infection of a mammalian host, Plasmodium parasites first replicate inside hepatocytes, generating thousands of new parasites. Although Plasmodium intra-hepatic development represents a substantial metabolic challenge to the host hepatocyte, how infected cells respond to and integrate this stress remains poorly understood. Here, we present proteomic and transcriptomic analyses, revealing that the endoplasmic reticulum ( ER)-resident unfolded protein response ( UPR) is activated in host hepatocytes upon Plasmodium berghei infection. The expression of XBP1s-the active form of the UPR mediator XBP1-and the liver-specific UPR mediator CREBH is induced by P. berghei infection in vivo. Furthermore, this UPR induction increases parasite liver burden. Altogether, our data suggest that ER stress is a central feature of P. berghei intra-hepatic development, contributing to the success of infection. [ABSTRACT FROM AUTHOR]
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- 2015
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7. Plasma Leptin Levels in Children Hospitalized with Cholera in Bangladesh.
- Author
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Falkard, Brie, Uddin, Taher, Rahman, M. Arifur, Franke, Molly F., Aktar, Amena, Uddin, Muhammad Ikhtear, Bhuiyan, Taufiqur Rahman, Leung, Daniel T., Charles, Richelle C., Larocque, Regina C., Harris, Jason B., Calderwood, Stephen B., Qadri, Firdausi, and Ryan, Edward T.
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- 2015
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8. A key role for lipoic acid synthesis during Plasmodium liver stage development.
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Falkard, Brie, Kumar, T. R. Santha, Hecht, Leonie‐Sophie, Matthews, Krista A., Henrich, Philipp P., Gulati, Sonia, Lewis, Rebecca E., Manary, Micah J., Winzeler, Elizabeth A., Sinnis, Photini, Prigge, Sean T., Heussler, Volker, Deschermeier, Christina, and Fidock, David
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LIPOIC acid , *MOSQUITO vectors , *METABOLITE synthesis , *PLASMODIUM berghei , *SHORT-chain fatty acids , *SPOROZOITES ,LIVER parasites - Abstract
The successful navigation of malaria parasites through their life cycle, which alternates between vertebrate hosts and mosquito vectors, requires a complex interplay of metabolite synthesis and salvage pathways. Using the rodent parasite Plasmodium berghei, we have explored the synthesis and scavenging pathways for lipoic acid, a short-chain fatty acid derivative that regulates the activity of α-ketoacid dehydrogenases including pyruvate dehydrogenase. In Plasmodium, lipoic acid is either synthesized de novo in the apicoplast or is scavenged from the host into the mitochondrion. Our data show that sporozoites lacking the apicoplast lipoic acid protein ligase LipB are markedly attenuated in their infectivity for mice, and in vitro studies document a very late liver stage arrest shortly before the final phase of intra-hepaticparasite maturation. LipB-deficient asexual blood stage parasites show unimpaired rates of growth in normal in vitro or in vivo conditions. However, these parasites showed reduced growth in lipid-restricted conditions induced by treatment with the lipoic acid analogue 8-bromo-octanoate or with the lipid-reducing agent clofibrate. This finding has implications for understanding Plasmodium pathogenesis in malnourished children that bear the brunt of malarial disease. This study also highlights the potential of exploiting lipid metabolism pathways for the design of genetically attenuated sporozoite vaccines. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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9. Oleic Acid Biosynthesis in Plasmodium falciparum: Characterization of the Stearoyl-CoA Desaturase and Investigation as a Potential Therapeutic Target.
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Gratraud, Paul, Huws, Enlli, Falkard, Brie, Adjalley, Sophie, Fidock, David A., Berry, Laurence, Jacobs Jr., William R., Baird, Mark S., Vial, Henri, and Kremer, Laurent
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OLEIC acid ,BIOSYNTHESIS ,PLASMODIUM falciparum ,ERYTHROCYTES ,PARASITES ,FATTY acids - Abstract
Background: Plasmodium falciparum parasitization of erythrocytes causes a substantial increase in the levels of intracellular fatty acids, notably oleic acid. How parasites acquire this monounsaturated fatty acid has remained enigmatic. Here, we report on the biochemical and enzymatic characterization of stearoyl-CoA desaturase (SCD) in P. falciparum. Methodology/Principal Findings: Metabolic labeling experiments allowed us to demonstrate the production of oleic acid from stearic acid both in lysates of parasites incubated with [
14 C]-stearoyl-CoA and in parasite-infected erythrocytes labeled with [14 C]-stearic acid. Optimal SCD activity was detected in schizonts, the stage of maximal membrane synthesis. This activity correlated with a late trophozoite stage-specific induction of PFE0555w transcripts. PFE0555w harbors a typical SCD signature. Similar to mammalian SCDs, this protein was found to be associated with the endoplasmic reticulum, as determined with PFE0555w-GFP tagged transgenic P. falciparum. Importantly, these parasites exhibited increased rates of stearic to oleic acid conversion, providing additional evidence that PFE0555w encodes the plasmodial SCD (PfSCD). These findings prompted us to assess the activity of sterculic acid analogues, known to be specific Δ9-desaturase inhibitors. Methyl sterculate inhibited the synthesis of oleic acid both with parasite lysates and infected erythrocytes, most likely by targeting PfSCD. This compound exhibited significant, rapid and irreversible antimalarial activity against asexual blood stages. This parasiticidal effect was antagonized by oleic acid. Conclusion/Significance: Our study provides evidence that parasite-mediated fatty acid modification is important for blood-stage survival and provides a new strategy to develop a novel antimalarial therapeutic based on the inhibition of PfSCD. [ABSTRACT FROM AUTHOR]- Published
- 2009
- Full Text
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10. Inhibition of HIV-1 replication by eIF3f.
- Author
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VaIente, Susana T., Gilmartin, Greg M., Mott, Christina, Falkard, Brie, and Goff, Stephen P.
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GENETIC testing ,VIRAL replication ,GENETIC translation ,MESSENGER RNA ,GENES - Abstract
Viruses often use host machinery in unusual ways to execute different steps during their replication. To identify host factors critical for virus replication, we screened cDNA expression libraries for genes or gene fragments that could interfere with HIV-1 vector transduction. The DNA clone that most potently inhibited HIV-1 expression encoded the N-terminal 91 aa of the eukaryotic initiation factor 3 subunit f (N91-elF3f). Overexpression of N91-elF3f or full-length elF3f drastically restricted HIV-1 replication by reducing nuclear and cytoplasmic viral mRNA levels. N91-eIF3f and eIF3f specifically targeted the 3' long terminal repeat (3'LTR) region in the viral mRNA. We show that the 3' end cleavage of HIV-1 mRNA precursors is specifically reduced in N91-elF3f expressing cells. Our results suggest a role of eIF3f in mRNA maturation and that it can specifically interfere with the 3' end processing of HIV-1 mRNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2009
11. The Fatty Acid Biosynthesis Enzyme FabI Plays a Key Role in the Development of Liver-Stage Malarial Parasites.
- Author
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Yu, Min, Kumar, T.R. Santha, Nkrumah, Louis J., Coppi, Alida, Retzlaff, Silke, Li, Celeste D., Kelly, Brendan J., Moura, Pedro A., Lakshmanan, Viswanathan, Freundlich, Joel S., Valderramos, Juan-Carlos, Vilcheze, Catherine, Siedner, Mark, Tsai, Jennifer H.-C., Falkard, Brie, Sidhu, Amar bir Singh, Purcell, Lisa A., Gratraud, Paul, Kremer, Laurent, and Waters, Andrew P.
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PLASMODIUM falciparum ,FATTY acid synthesis ,BIOSYNTHESIS ,ANTIMALARIALS ,VIRAL genetics ,LABORATORY rodents - Abstract
Summary: The fatty acid synthesis type II pathway has received considerable interest as a candidate therapeutic target in Plasmodium falciparum asexual blood-stage infections. This apicoplast-resident pathway, distinct from the mammalian type I process, includes FabI. Here, we report synthetic chemistry and transfection studies concluding that Plasmodium FabI is not the target of the antimalarial activity of triclosan, an inhibitor of bacterial FabI. Disruption of fabI in P. falciparum or the rodent parasite P. berghei does not impede blood-stage growth. In contrast, mosquito-derived, FabI-deficient P. berghei sporozoites are markedly less infective for mice and typically fail to complete liver-stage development in vitro. This defect is characterized by an inability to form intrahepatic merosomes that normally initiate blood-stage infections. These data illuminate key differences between liver- and blood-stage parasites in their requirements for host versus de novo synthesized fatty acids, and create new prospects for stage-specific antimalarial interventions. [Copyright &y& Elsevier]
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- 2008
- Full Text
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