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Inhibition of HIV-1 replication by eIF3f.
- Source :
- Proceedings of the National Academy of Sciences of the United States of America; 3/17/2009, Vol. 106 Issue 11, p4071-4078, 8p, 4 Graphs
- Publication Year :
- 2009
-
Abstract
- Viruses often use host machinery in unusual ways to execute different steps during their replication. To identify host factors critical for virus replication, we screened cDNA expression libraries for genes or gene fragments that could interfere with HIV-1 vector transduction. The DNA clone that most potently inhibited HIV-1 expression encoded the N-terminal 91 aa of the eukaryotic initiation factor 3 subunit f (N91-elF3f). Overexpression of N91-elF3f or full-length elF3f drastically restricted HIV-1 replication by reducing nuclear and cytoplasmic viral mRNA levels. N91-eIF3f and eIF3f specifically targeted the 3' long terminal repeat (3'LTR) region in the viral mRNA. We show that the 3' end cleavage of HIV-1 mRNA precursors is specifically reduced in N91-elF3f expressing cells. Our results suggest a role of eIF3f in mRNA maturation and that it can specifically interfere with the 3' end processing of HIV-1 mRNAs. [ABSTRACT FROM AUTHOR]
- Subjects :
- GENETIC testing
VIRAL replication
GENETIC translation
MESSENGER RNA
GENES
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 106
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 37343488