1. N 6 -Methyladenosine Modification-Related Genes Express Differentially in Sterile Male Cattle-Yaks.
- Author
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Liu, Yuxin, Chen, Lili, Jiang, Hui, Wang, Hongzhuang, Zhang, Yujiao, Yuan, Zhengrong, and Ma, Yi
- Subjects
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RNA-binding proteins , *MALE sterility in plants , *RNA modification & restriction , *SEMINIFEROUS tubules , *POLYMERASE chain reaction , *SPERMATOGENESIS - Abstract
N6-methyladenosine (m6A), an RNA post-transcriptional modification, plays a crucial role in spermatogenesis. Cattle-yaks are interspecific hybrid offsprings of yak and cattle, and male cattle-yaks are sterile. This study aims to investigate the role of m6A modification in male cattle-yak infertility. Herein, testicular tissues were analyzed via histological observations, immunohistochemical assays, reverse-transcription quantitative polymerase chain reaction, Western blotting, and immunofluorescence assays. The results revealed that male cattle-yaks presented smaller testes (5.933 ± 0.4885 cm vs. 7.150 ± 0.3937 cm), with only single cell layers in seminiferous tubules, and weakened signals of m6A regulators such as METTL14 (methyltransferase-like 14), ALKBH5 (alpha-ketoglutarate-dependent hydroxylase homolog 5), FTO (fat mass and obesity-associated protein), and YTHDF2 (YTH N6-methyladenosine RNA binding protein F2), both at the RNA and protein levels, compared with those of yaks. Altogether, these findings suggest that m6A modification may play a crucial role in male cattle-yak sterility, providing a basis for future studies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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