98 results on '"Ribeiro, Jose M. C."'
Search Results
2. Pf CDPK1 is critical for malaria parasite gametogenesis and mosquito infection
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Bansal, Abhisheka, Molina-Cruz, Alvaro, Brzostowski, Joseph, Liu, Poching, Luo, Yan, Gunalan, Karthigayan, Li, Yuesheng, Ribeiro, José M. C., and Miller, Louis H.
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- 2018
3. Tick innate immune responses to hematophagy and Ehrlichia infection at single-cell resolution.
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Adegoke, Abdulsalam, Ribeiro, Jose M. C., Smith, Ryan C., and Karim, Shahid
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EHRLICHIOSIS ,MOLECULAR biology ,RNA interference ,IMMUNE response ,SMALL interfering RNA - Abstract
Introduction: Ticks rely on robust cellular and humoral responses to control microbial infection. However, several aspects of the tick's innate immune system remain uncharacterized, most notably that of the immune cells (called hemocytes), which are known to play a significant role in cellular and humoral responses. Despite the importance of hemocytes in regulating microbial infection, our understanding of their basic biology and molecular mechanisms remains limited. Therefore, we believe that a more detailed understanding of the role of hemocytes in the interactions between ticks and tick-borne microbes is crucial to illuminating their function in vector competence and to help identify novel targets for developing new strategies to block tick-borne pathogen transmission. Methods: This study examined hemocytes from the lone star tick (Amblyomma americanum) at the transcriptomic level using the 10X genomics single-cell RNA sequencing platform to analyze hemocyte populations from unfed, partially blood-fed, and Ehrlichia chaffeensis-infected ticks. The functional role of differentially expressed hemocyte markers in hemocyte proliferation and Ehrlichia dissemination was determined using an RNA interference approach. Results and discussion: Our data exhibit the identification of fourteen distinct hemocyte populations. Our results uncover seven distinct lineages present in uninfected and Ehrlichia-infected hemocyte clusters. The functional characterization of hemocytin, cystatin, fibronectin, and lipocalin demonstrate their role in hemocyte population changes, proliferation, and Ehrlichia dissemination. Conclusion: Our results uncover the tick immune responses to Ehrlichia infection and hematophagy at a single-cell resolution. This work opens a new field of tick innate immunobiology to understand the role of hemocytes, particularly in response to prolonged blood-feeding (hematophagy), and tickmicrobial interactions. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
- View/download PDF
4. Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection
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Mesquita, Rafael D., Vionette-Amaral, Raquel J., Lowenberger, Carl, Rivera-Pomar, Rolando, Monteiro, Fernando A., Minx, Patrick, Spieth, John, Carvalho, A. Bernardo, Panzera, Francisco, Lawson, Daniel, Torres, André Q., Ribeiro, Jose M. C., Sorgine, Marcos H. F., Waterhouse, Robert M., Montague, Michael J., Abad-Franch, Fernando, Alves-Bezerra, Michele, Amaral, Laurence R., Araujo, Helena M., Araujo, Ricardo N., Aravind, L., Atella, Georgia C., Azambuja, Patricia, Berni, Mateus, Bittencourt-Cunha, Paula R., Braz, Gloria R. C., Calderón-Fernández, Gustavo, Carareto, Claudia M. A., Christensen, Mikkel B., Costa, Igor R., Costa, Samara G., Dansa, Marilvia, Daumas-Filho, Carlos R. O., De-Paula, Iron F., Dias, Felipe A., Dimopoulos, George, Emrich, Scott J., Esponda-Behrens, Natalia, Fampa, Patricia, Fernandez-Medina, Rita D., da Fonseca, Rodrigo N., Fontenele, Marcio, Fronick, Catrina, Fulton, Lucinda A., Gandara, Ana Caroline, Garcia, Eloi S., Genta, Fernando A., Giraldo-Calderón, Gloria I., Gomes, Bruno, Gondim, Katia C., Granzotto, Adriana, Guarneri, Alessandra A., Guigó, Roderic, Harry, Myriam, Hughes, Daniel S. T., Jablonka, Willy, Jacquin-Joly, Emmanuelle, Juárez, M. Patricia, Koerich, Leonardo B., Lange, Angela B., Latorre-Estivalis, José Manuel, Lavore, Andrés, Lawrence, Gena G., Lazoski, Cristiano, Lazzari, Claudio R., Lopes, Raphael R., Lorenzo, Marcelo G., Lugon, Magda D., Majerowicz, David, Marcet, Paula L., Mariotti, Marco, Masuda, Hatisaburo, Megy, Karine, Melo, Ana C. A., Missirlis, Fanis, Mota, Theo, Noriega, Fernando G., Nouzova, Marcela, Nunes, Rodrigo D., Oliveira, Raquel L. L., Oliveira-Silveira, Gilbert, Ons, Sheila, Orchard, Ian, Pagola, Lucia, Paiva-Silva, Gabriela O., Pascual, Agustina, Pavan, Marcio G., Pedrini, Nicolás, Peixoto, Alexandre A., Pereira, Marcos H., Pike, Andrew, Polycarpo, Carla, Prosdocimi, Francisco, Ribeiro-Rodrigues, Rodrigo, Robertson, Hugh M., Salerno, Ana Paula, Salmon, Didier, Santesmasses, Didac, Schama, Renata, Seabra-Junior, Eloy S., Silva-Cardoso, Livia, Silva-Neto, Mario A. C., Souza-Gomes, Matheus, Sterkel, Marcos, Taracena, Mabel L., Tojo, Marta, Tu, Zhijian Jake, Tubio, Jose M. C., Ursic-Bedoya, Raul, Venancio, Thiago M., Walter-Nuno, Ana Beatriz, Wilson, Derek, Warren, Wesley C., Wilson, Richard K., Huebner, Erwin, Dotson, Ellen M., and Oliveira, Pedro L.
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- 2015
5. Functional and structural similarities of D7 proteins in the independently-evolved salivary secretions of sand flies and mosquitoes
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Jablonka, Willy, Kim, Il Hwan, Alvarenga, Patricia H., Valenzuela, Jesus G., Ribeiro, Jose´ M. C., and Andersen, John F.
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- 2019
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6. Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti
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Chagas, Andrezza Campos, Ramirez, José Luis, Jasinskiene, Nijole, James, Anthony A., Ribeiro, José M. C., Marinotti, Osvaldo, and Calvo, Eric
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- 2014
7. The king cobra genome reveals dynamic gene evolution and adaptation in the snake venom system
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Vonk, Freek J., Casewell, Nicholas R., Henkel, Christiaan V., Heimberg, Alysha M., Jansen, Hans J., McCleary, Ryan J. R., Kerkkamp, Harald M. E., Vos, Rutger A., Guerreiro, Isabel, Calvete, Juan J., Wüster, Wolfgang, Woods, Anthony E., Logan, Jessica M., Harrison, Robert A., Castoe, Todd A., de Koning, A. P. Jason, Pollock, David D., Yandell, Mark, Calderon, Diego, Renjifo, Camila, Currier, Rachel B., Salgado, David, Pla, Davinia, Sanz, Libia, Hyder, Asad S., Ribeiro, José M. C., Arntzen, Jan W., van den Thillart, Guido E. E. J. M., Boetzer, Marten, Pirovano, Walter, Dirks, Ron P., Spaink, Herman P., Duboule, Denis, McGlinn, Edwina, Kini, Manjunatha, and Richardson, Michael K.
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- 2013
8. Rickettsia parkeri hijacks tick hemocytes to manipulate cellular and humoral transcriptional responses.
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Adegoke, Abdulsalam, Ribeiro, Jose M. C., Brown, Sidney, Smith, Ryan C., and Karim, Shahid
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BLOOD cells ,MOLECULAR biology ,PHAGOCYTOSIS ,RICKETTSIA ,HUMORAL immunity ,TICKS ,BIOMARKERS - Abstract
Introduction: Blood-feeding arthropods rely on robust cellular and humoral immunity to control pathogen invasion and replication. Tick hemocytes produce factors that can facilitate or suppress microbial infection and pathogenesis. Despite the importance of hemocytes in regulating microbial infection, understanding of their basic biology and molecular mechanisms remains limited. Methods: Here we combined histomorphology and functional analysis to identify five distinct phagocytic and non-phagocytic hemocyte populations circulating within the Gulf Coast tick Amblyomma maculatum. Results and discussion: Depletion of phagocytic hemocytes using clodronate liposomes revealed their function in eliminating bacterial infection. We provide the first direct evidence that an intracellular tick-borne pathogen, Rickettsia parkeri, infects phagocytic hemocytes in Am. maculatum to modify tick cellular immune responses. A hemocyte-specific RNA-seq dataset generated from hemocytes isolated from uninfected and R. parkeri-infected partially blood-fed ticks generated ~40,000 differentially regulated transcripts, >11,000 of which were immune genes. Silencing two differentially regulated phagocytic immune marker genes (nimrod B2 and eater-two Drosophila homologs), significantly reduced hemocyte phagocytosis. Conclusion: Together, these findings represent a significant step forward in understanding how hemocytes regulate microbial homeostasis and vector competence. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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9. Stage-specific proteomic expression patterns of the human filarial parasite Brugia malayi and its endosymbiont Wolbachia
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Bennuru, Sasisekhar, Meng, Zhaojing, Ribeiro, José M. C., Semnani, Roshanak Tolouei, Ghedin, Elodie, Chan, King, Lucas, David A., Veenstra, Timothy D., and Nutman, Thomas B.
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- 2011
10. Sequencing of Culex quinquefasciatus Establishes a Platform for Mosquito Comparative Genomics
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Arensburger, Peter, Megy, Karine, Waterhouse, Robert M., Abrudan, Jenica, Amedeo, Paolo, Antelo, Beatriz, Bartholomay, Lyric, Bidwell, Shelby, Caler, Elisabet, Camara, Francisco, Campbell, Corey L., Campbell, Kathryn S., Casola, Claudio, Castro, Marta T., Chandramouliswaran, Ishwar, Chapman, Sinéad B., Christley, Scott, Costas, Javier, Eisenstadt, Eric, Feschotte, Cedric, Fraser-Liggett, Claire, Guigo, Roderic, Haas, Brian, Hammond, Martin, Hansson, Bill S., Hemingway, Janet, Hill, Sharon R., Howarth, Clint, Ignell, Rickard, Kennedy, Ryan C., Kodira, Chinnappa D., Lobo, Neil F., Mao, Chunhong, Mayhew, George, Michel, Kristin, Mori, Akio, Liu, Nannan, Naveira, Horado, Nene, Vishvanath, Nguyen, Nam, Pearson, Matthew D., Pritham, Ellen J., Puiu, Daniela, Qi, Yumin, Ranson, Hilary, Ribeiro, Jose M. C., Roberston, Hugh M., Severson, David W., Shumway, Martin, Stanke, Mario, Strausberg, Robert L., Sun, Cheng, Sutton, Granger, Tubio, Jose Manuel C., Unger, Maria F., Vanlandingham, Dana L., Vilella, Albert J., White, Owen, White, Jared R., Wondji, Charles S., Wortman, Jennifer, Zdobnov, Evgeny M., Birren, Bruce, Christensen, Bruce M., Collins, Frank H., Cornel, Anthony, Dimopoulos, George, Hannick, Linda I., Higgs, Stephen, Lanzaro, Gregory C., Lawson, Daniel, Lee, Norman H., Muskavitch, Marc A. T., Raikhel, Alexander S., and Atkinson, Peter W.
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- 2010
11. Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle
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Kirkness, Ewen F., Haas, Brian J., Sun, Weilin, Braig, Henk R., Perotti, M. Alejandra, Clark, John M., Lee, Si Hyeock, Robertson, Hugh M., Kennedy, Ryan C., Elhaik, Eran, Gerlach, Daniel, Kriventseva, Evgenia V., Elsik, Christine G., Graur, Dan, Hill, Catherine A., Veenstra, Jan A., Walenz, Brian, Tubío, José Manuel C., Ribeiro, José M. C., Rozas, Julio, Johnston, J. Spencer, Reese, Justin T., Popadic, Aleksandar, Tojo, Marta, Raoult, Didier, Reed, David L., Tomoyasu, Yoshinori, Krause, Emily, Mittapalli, Omprakash, Margam, Venu M., Li, Hong-Mei, Meyer, Jason M., Johnson, Reed M., Romero-Severson, Jeanne, VanZee, Janice Pagel, Alvarez-Ponce, David, Vieira, Filipe G., Aguadé, Montserrat, Guirao-Rico, Sara, Anzola, Juan M., Yoon, Kyong S., Strycharz, Joseph P., Unger, Maria F., Christley, Scott, Lobo, Neil F., Seufferheld, Manfredo J., Wang, NaiKuan, Dasch, Gregory A., Struchiner, Claudio J., Madey, Greg, Hannick, Linda I., Bidwell, Shelby, Joardar, Vinita, Caler, Elisabet, Shao, Renfu, Barker, Stephen C., Cameron, Stephen, Bruggner, Robert V., Regier, Allison, Johnson, Justin, Viswanathan, Lakshmi, Utterback, Terry R., Sutton, Granger G., Lawson, Daniel, Waterhouse, Robert M., Venter, J. Craig, Strausberg, Robert L., Berenbaum, May R., Collins, Frank H., Zdobnov, Evgeny M., and Pittendrigh, Barry R.
- Published
- 2010
12. The Tempo and Mode of Evolution of Transposable Elements as Revealed by Molecular Phylogenies Reconstructed from Mosquito Genomes
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Struchiner, Claudio J., Massad, Eduardo, Tu, Zhijian, and Ribeiro, José M. C.
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- 2009
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13. Multifunctionality and Mechanism of Ligand Binding in a Mosquito Antiinflammatory Protein
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Calvo, Eric, Mans, Ben J., Ribeiro, José M. C., Andersen, John F., and James, Anthony A.
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- 2009
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14. Rickettsia parkeri infection modulates the sialome and ovariome of the Gulf coast tick, Amblyomma maculatum.
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Garcia Guizzo, Melina, Budachetri, Khemraj, Adegoke, Abdulsalam, Ribeiro, Jose M. C., and Karim, Shahid
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AMBLYOMMA ,RICKETTSIA ,TICKS ,SALIVARY glands ,CANDIDATUS - Abstract
The Gulf Coast tick, Amblyomma maculatum, is a vector of several tick-borne pathogens, including Rickettsia parkeri. The ability of R. parkeri to persist within the tick population through transovarial and transstadial transmission, without apparently harming the ticks, contributes to the pathogen's perpetuation in the tick population. Previous studies have shown that the R. parkeri load in A. maculatum is regulated by the tick tissues' oxidant/antioxidant balance and the non-pathogenic tick microbiome. To obtain further insights into the interaction between tick and pathogen, we performed a bulk RNA-Seq for differential transcriptomic analysis of ovaries and salivary glands from R. parkeri-infected and uninfected ticks over the feeding course on a host. The most differentially expressed functional category was of bacterial origin, exhibiting a massive overexpression of bacterial transcripts in response to the R. parkeri infection. Candidatus Midichloria mitochondrii and bacteria from the genus Rickettsia were mainly responsible for the overexpression of bacterial transcripts. Host genes were also modulated in R. parkeri-infected tick organs. A similar number of host transcripts from all analyzed functional categories was negatively and positively modulated, revealing a global alteration of the A. maculatum transcriptome in response to pathogen infection. R. parkeri infection led to an increase in salivary transcripts involved in blood feeding success as well as a decrease in ovarian immune transcripts. We hypothesize that these transcriptional alterations facilitate pathogen persistence and transmission within tick population. [ABSTRACT FROM AUTHOR]
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- 2022
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15. dCAS: a desktop application for cDNA sequence annotation
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Guo, Yongjian, Ribeiro, Jose M. C., Anderson, Jennifer M., and Bour, Stephan
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- 2009
16. Proteomics Pipeline for Identifying Variant Proteins in Plasmodium falciparum Parasites Isolated from Children Presenting with Malaria.
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Gonzales Hurtado, Patricia A., Morrison, Robert, Ribeiro, Jose M. C., Magale, Hussein, Attaher, Oumar, Diarra, Bacary S., Mahamar, Almahamoudou, Barry, Amadou, Dicko, Alassane, Duffy, Patrick E., and Fried, Michal
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- 2019
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17. Scaling of Feeding and Reproductive Structures in the Mosquito Aedes aegypti L. (Diptera: Culicidae)
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Wheeler, Diana, Wong, Audric, and Ribeiro, José M. C.
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- 1993
18. Erratum to :'Genome of Rhodnius prolixus, an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection' (vol 112, pg 14936, 2015)
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Lowenberger, Carl, Rivera Pomar, Rolando, Monteiro, Fernando A., Minx, Patrick, Spieth, John, Bernardo Carvalho, A., Panzera, Francisco, Lawson, Daniel, Torres, Andre Q., Ribeiro, Jose M. C., Sorgine, Marcos H. F., Waterhouse, Robert M., Montague, Michael J., Abad Franch, Fernando, Alves Bezerra, Michele, Amaral, Laurence R., Araujo, Helena M., Araujo, Ricardo N., Aravind, L., Atella, Georgia C., Azambuja, Patricia, Berni, Mateus, Bittencourt Cunha, Paula R., Braz, Gloria R. C., Calderon Fernandez, Gustavo, Carareto, Claudia M. A., Christensen, Mikkel B., Costa, Igor R., Costa, Samara G., Dansa, Marilvia, Daumas Filho, Carlos R. O., De Paula, Iron F., Dias, Felipe A., Dimopoulos, George, Emrich, Scott J., Esponda Behrens, Natalia, Fampa, Patricia, Fernandez Medina, Rita D., da Fonseca, Rodrigo N., Fontenele, Marcio, Fronick, Catrina, Fulton, Lucinda A., Gandara, Ana Caroline, Garcia, Eloi S., Genta, Fernando A., Giraldo Calderón, Gloria I., Gomes, Bruno, Gondim, Katia C., Granzotto, Adriana, Guarneri, Alessandra A., Guigó, Roderic, Harry, Myriam, Hughes, Daniel S. T., Jablonka, Willy, Jacquin Joly, Emmanuelle, Patricia Juarez, M., Koerich, Leonardo B., Latorre Estivalis, Jose Manuel, Lavore, Andrés, Lawrence, Gena G., Lazoski, Cristiano, Lazzari, Claudio R., Lopes, Raphael R., Lorenzo, Marcelo G., Lugon, Magda D., Majerowicz, David, Marcet, Paula L., Mariotti, Marco, Masuda, Hatisaburo, Megy, Karine, Melo, Ana C.A., Missirlis, Fanis, Mota, Theo, Noriega, Fernando G., Nouzova, Marcela, Nunes, Rodrigo D., Oliveira, Raquel L.L., Oliveira Silveira, Gilbert, Ons, Sheila, Pagola, Lucia, Paiva Silva, Gabriela O., Pascual, Agustina, Pavan, Marcio G., Pedrini, Nicolas, Peixoto, Alexandre A., Pereira, Marcos H., Pike, Andrew, Polycarpo, Carla, Prosdocimi, Francisco, Ribeiro Rodrigues, Rodrigo, Robertson, Hugh M., Salerno, Ana Paula, Salmon, Didier, Santesmasses, Didac, Schama, Renata, Seabra Junior, Eloy S., Silva Cardoso, Livia, Mesquita, Rafael D., and Vionette Amaral, Raquel J.
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rhodnius prolixus ,Rhodnius prolixus ,genome ,hematophagy ,immunity ,Chagas disease ,maladie de chagas - Abstract
Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (similar to 702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.
- Published
- 2016
19. Immunity to LuloHya and Lundep, the salivary spreading factors from Lutzomyia longipalpis, protects against Leishmania major infection.
- Author
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Martin-Martin, Ines, Chagas, Andrezza Campos, Guimaraes-Costa, Anderson B., Amo, Laura, Oliveira, Fabiano, Moore, Ian N., DeSouza-Vieira, Thiago S., Sanchez, Elda E., Suntravat, Montamas, Valenzuela, Jesus G., Ribeiro, Jose M. C., and Calvo, Eric
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LUTZOMYIA ,LEISHMANIA ,SALIVARY glands ,ARTHROPOD vectors ,BLOOD meal as feed ,ENDONUCLEASES - Abstract
Salivary components from disease vectors help arthropods to acquire blood and have been shown to enhance pathogen transmission in different model systems. Here we show that two salivary enzymes from Lutzomyia longipalpis have a synergist effect that facilitates a more efficient blood meal intake and diffusion of other sialome components. We have previously shown that Lundep, a highly active endonuclease, enhances parasite infection and prevent blood clotting by inhibiting the intrinsic pathway of coagulation. To investigate the physiological role of a salivary hyaluronidase in blood feeding we cloned and expressed a recombinant hyaluronidase from Lu. longipalpis. Recombinant hyaluronidase (LuloHya) was expressed in mammalian cells and biochemically characterized in vitro. Our study showed that expression of neutrophil CXC chemokines and colony stimulating factors were upregulated in HMVEC cells after incubation with LuloHya and Lundep. These results were confirmed by the acute hemorrhage, edema and inflammation in a dermal necrosis (dermonecrotic) assay involving a massive infiltration of leukocytes, especially neutrophils, in mice co-injected with hemorrhagic factor and these two salivary proteins. Moreover, flow cytometry results showed that LuloHya and Lundep promote neutrophil recruitment to the bite site that may serve as a vehicle for establishment of Leishmania infection. A vaccination experiment demonstrated that LuloHya and Lundep confer protective immunity against cutaneous leishmaniasis using the Lu. longipalpis—Leishmania major combination as a model. Animals (C57BL/6) immunized with LuloHya or Lundep showed minimal skin damage while lesions in control animals remained ulcerated. This protective immunity was abrogated when B-cell-deficient mice were used indicating that antibodies against both proteins play a significant role for disease protection. Rabbit-raised anti-LuloHya antibodies completely abrogated hyaluronidase activity in vitro. Moreover, in vivo experiments demonstrated that blocking LuloHya with specific antibodies interferes with sand fly blood feeding. This work highlights the relevance of vector salivary components in blood feeding and parasite transmission and further suggests the inclusion of these salivary proteins as components for an anti-Leishmania vaccine. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
20. Comparative analysis of the global transcriptome of Anopheles funestus from Mali, West Africa
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Serazin, Aandrew C., Dana, Ali N., Hillenmeyer, Maureen E., Lobo, Neil F., Coulibaly, Mamadou B., Willard, Michael B., Harker, Brent W., Sharakhov, Igor V., Collins, Frank H., Ribeiro, Jose M. C., Besansky, Nora J., and Entomology
- Subjects
Expressed Sequence Tags ,DNA, Complementary ,Transcription, Genetic ,Gene Expression Profiling ,Computational Biology ,Apoptosis ,Genomics ,Mali ,Salivary Glands ,Malaria ,Mitochondria ,parasitic diseases ,Anopheles ,Animals ,Female ,Gene Library - Abstract
BACKGROUND: Anopheles funestus is a principal vector of malaria across much of tropical Africa and is considered one of the most efficient of its kind, yet studies of this species have lagged behind those of its broadly sympatric congener, An. gambiae. In aid of future genomic sequencing of An. funestus, we explored the whole body transcriptome, derived from mixed stage progeny of wild-caught females from Mali, West Africa. PRINCIPAL FINDINGS: Here we report the functional annotation and comparative genomics of 2,005 expressed sequence tags (ESTs) from An. funestus, which were assembled with a previous EST set from adult female salivary glands from the same mosquito. The assembled ESTs provided for a nonredundant catalog of 1,035 transcripts excluding mitochondrial sequences. CONCLUSIONS/SIGNIFICANCE: Comparison of the An. funestus and An. gambiae transcriptomes using computational and macroarray approaches revealed a high degree of sequence identity despite an estimated 20-80 MY divergence time between lineages. A phylogenetically broader comparative genomic analysis indicated that the most rapidly evolving proteins--those involved in immunity, hematophagy, formation of extracellular structures, and hypothetical conserved proteins--are those that probably play important roles in how mosquitoes adapt to their nutritional and external environments, and therefore could be of greatest interest in disease control. Published version
- Published
- 2009
21. Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica.
- Author
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Abdeladhim, Maha, V. Coutinho-Abreu, Iliano, Townsend, Shannon, Pasos-Pinto, Silvia, Sanchez, Laura, Rasouli, Manoochehr, B. Guimaraes-Costa, Anderson, Aslan, Hamide, Francischetti, Ivo M. B., Oliveira, Fabiano, Becker, Ingeborg, Kamhawi, Shaden, Ribeiro, Jose M. C., Jochim, Ryan C., and Valenzuela, Jesus G.
- Subjects
BIODIVERSITY ,SALIVARY proteins ,SAND flies ,LEISHMANIA mexicana ,BIOMARKERS - Abstract
Background: Sand fly saliva has been shown to have proteins with potent biological activities, salivary proteins that can be used as biomarkers of vector exposure, and salivary proteins that are candidate vaccines against different forms of leishmaniasis. Sand fly salivary gland transcriptomic approach has contributed significantly to the identification and characterization of many of these salivary proteins from important Leishmania vectors; however, sand fly vectors in some regions of the world are still neglected, as Bichromomyia olmeca (formerly known as Lutzomyia olmeca olmeca), a proven vector of Leishmania mexicana in Mexico and Central America. Despite the importance of this vector in transmitting Leishmania parasite in Mesoamerica there is no information on the repertoire of B. olmeca salivary proteins and their relationship to salivary proteins from other sand fly species. Methods and Findings: A cDNA library of the salivary glands of wild-caught B. olmeca was constructed, sequenced, and analyzed. We identified transcripts encoding for novel salivary proteins from this sand fly species and performed a comparative analysis between B. olmeca salivary proteins and those from other sand fly species. With this new information we present an updated catalog of the salivary proteins specific to New World sand flies and salivary proteins common to all sand fly species. We also report in this work the anti-Factor Xa activity of Lofaxin, a salivary anticoagulant protein present in this sand fly species. Conclusions: This study provides information on the first transcriptome of a sand fly from Mesoamerica and adds information to the limited repertoire of salivary transcriptomes from the Americas. This comparative analysis also shows a fast degree of evolution in salivary proteins from New World sand flies as compared with Old World sand flies. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
22. The structure of hookworm platelet inhibitor (HPI), a CAP superfamily member from Ancylostoma caninum.
- Author
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Ma, Dongying, Francischetti, Ivo M. B., Ribeiro, Jose M. C., and Andersen, John F.
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ANCYLOSTOMA caninum ,HOOKWORMS ,PLATELET aggregation inhibitors ,CYSTEINE ,ANTIGENS - Abstract
Secreted protein components of hookworm species include a number of representatives of the cysteine-rich/antigen 5/pathogenesis-related 1 (CAP) protein family known as Ancylostoma-secreted proteins (ASPs). Some of these have been considered as candidate antigens for the development of vaccines against hookworms. The functions of most CAP superfamily members are poorly understood, but one form, the hookworm platelet inhibitor (HPI), has been isolated as a putative antagonist of the platelet integrins α
IIb β3 and α2 β1 . Here, the crystal structure of HPI is described and its structural features are examined in relation to its possible function. The HPI structure is similar to those of other ASPs and shows incomplete conservation of the sequence motifs CAP1 and CAP2 that are considered to be diagnostic of CAP superfamily members. The asymmetric unit of the HPI crystal contains a dimer with an extensive interaction interface, but chromatographic measurements indicate that it is primarily monomeric in solution. In the dimeric structure, the putative active-site cleft areas from both monomers are united into a single negatively charged depression. A potential Lys-Gly-Asp disintegrin-like motif was identified in the sequence of HPI, but is not positioned at the apex of a tight turn, making it unlikely that it interacts with the integrin. Recombinant HPI produced in Escherichia coli was found not to inhibit the adhesion of human platelets to collagen or fibrinogen, despite having a native structure as shown by X-ray diffraction. This result corroborates previous analyses of recombinant HPI and suggests that it might require post-translational modification or have a different biological function. [ABSTRACT FROM AUTHOR]- Published
- 2015
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- View/download PDF
23. Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis.
- Author
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Vlkova, Michaela, Sima, Michal, Rohousova, Iva, Kostalova, Tatiana, Sumova, Petra, Volfova, Vera, Jaske, Erin L., Barbian, Kent D., Gebre-Michael, Teshome, Hailu, Asrat, Warburg, Alon, Ribeiro, Jose M. C., Valenzuela, Jesus G., Jochim, Ryan C., and Volf, Petr
- Subjects
SAND flies ,VISCERAL leishmaniasis ,PHLEBOTOMUS ,SALIVARY glands ,SALIVARY proteins - Abstract
Background: In East Africa, Phlebotomus orientalis serves as the main vector of Leishmania donovani, the causative agent of visceral leishmaniasis (VL). Phlebotomus orientalis is present at two distant localities in Ethiopia; Addis Zemen where VL is endemic and Melka Werer where transmission of VL does not occur. To find out whether the difference in epidemiology of VL is due to distant compositions of P. orientalis saliva we established colonies from Addis Zemen and Melka Werer, analyzed and compared the transcriptomes, proteomes and enzymatic activity of the salivary glands. Methodology/Principal Findings: Two cDNA libraries were constructed from the female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Clones of each P. orientalis library were randomly selected, sequenced and analyzed. In P. orientalis transcriptomes, we identified members of 13 main protein families. Phylogenetic analysis and multiple sequence alignments were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. To further compare both colonies, we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Conclusions: This is the first report of the salivary components of P. orientalis, an important vector sand fly. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. Based on the phylogenetic analysis, we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We also demonstrated that there is no significant difference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen (endemic area) and Melka Werer (non-endemic area) P. orientalis colonies. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland composition. Author Summary: Phlebotomus orientalis is the vector of visceral leishmaniasis (VL) caused by Leishmania donovani in Northeast Africa. Immunization with sand fly saliva or with individual salivary proteins has been shown to protect against leishmaniasis in different hosts, warranting the intensive study of salivary proteins of sand fly vectors. In our study, we characterize the salivary compounds of P. orientalis, thereby broadening the repertoire of salivary proteins of sand fly species belonging to the subgenus Larroussius. In order to find out whether there is any connection between the composition of P. orientalis saliva and the epidemiology of VL in two distinct Ethiopian foci, Addis Zemen and Melka Werer, we studied the transcriptomes, proteomes, enzymatic activities, and the main salivary antigens in two P. orientalis colonies originating from these areas. We did not detect any significant difference between the saliva of female sand flies originating in Addis Zemen (endemic area) and Melka Werer (non-endemic area). Therefore, the different epidemiology of VL in these Ethiopian foci cannot be related to the distant salivary gland protein composition. Identifying the sand fly salivary gland compounds will be useful for future research focused on characterizing suitable salivary proteins as potential anti-Leishmania vaccine candidates. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
24. Comparative Analysis of Salivary Gland Transcriptomes of Phlebotomus orientalis Sand Flies from Endemic and Non-endemic Foci of Visceral Leishmaniasis.
- Author
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Vlkova, Michaela, Sima, Michal, Rohousova, Iva, Kostalova, Tatiana, Sumova, Petra, Volfova, Vera, Jaske, Erin L., Barbian, Kent D., Gebre-Michael, Teshome, Hailu, Asrat, Warburg, Alon, Ribeiro, Jose M. C., Valenzuela, Jesus G., Jochim, Ryan C., and Volf, Petr
- Subjects
SALIVARY glands ,PHLEBOTOMUS ,PSYCHODIDAE ,EXOCRINE glands ,INFECTIOUS disease transmission - Abstract
Background: In East Africa, Phlebotomus orientalis serves as the main vector of Leishmania donovani, the causative agent of visceral leishmaniasis (VL). Phlebotomus orientalis is present at two distant localities in Ethiopia; Addis Zemen where VL is endemic and Melka Werer where transmission of VL does not occur. To find out whether the difference in epidemiology of VL is due to distant compositions of P. orientalis saliva we established colonies from Addis Zemen and Melka Werer, analyzed and compared the transcriptomes, proteomes and enzymatic activity of the salivary glands. Methodology/Principal Findings: Two cDNA libraries were constructed from the female salivary glands of P. orientalis from Addis Zemen and Melka Werer. Clones of each P. orientalis library were randomly selected, sequenced and analyzed. In P. orientalis transcriptomes, we identified members of 13 main protein families. Phylogenetic analysis and multiple sequence alignments were performed to evaluate differences between the P. orientalis colonies and to show the relationship with other sand fly species from the subgenus Larroussius. To further compare both colonies, we investigated the humoral antigenicity and cross-reactivity of the salivary proteins and the activity of salivary apyrase and hyaluronidase. Conclusions: This is the first report of the salivary components of P. orientalis, an important vector sand fly. Our study expanded the knowledge of salivary gland compounds of sand fly species in the subgenus Larroussius. Based on the phylogenetic analysis, we showed that P. orientalis is closely related to Phlebotomus tobbi and Phlebotomus perniciosus, whereas Phlebotomus ariasi is evolutionarily more distinct species. We also demonstrated that there is no significant difference between the transcriptomes, proteomes or enzymatic properties of the salivary components of Addis Zemen (endemic area) and Melka Werer (non-endemic area) P. orientalis colonies. Thus, the different epidemiology of VL in these Ethiopian foci cannot be attributed to the salivary gland composition. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
25. Structure and ligand-binding properties of the biogenic amine-binding protein from the saliva of a blood-feeding insect vector of Trypanosoma cruzi.
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Xu, Xueqing, Chang, Bianca W., Mans, Ben J., Ribeiro, Jose M. C., and Andersen, John F.
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LIGAND binding (Biochemistry) ,HEMOSTASIS ,LIPOCALIN-1 ,TRYPANOSOMA cruzi ,CARRIER proteins ,BLOOD platelet activation ,BIOGENIC amines - Abstract
Proteins that bind small-molecule mediators of inflammation and hemostasis are essential for blood-feeding by arthropod vectors of infectious disease. In ticks and triatomine insects, the lipocalin protein family is greatly expanded and members have been shown to bind biogenic amines, eicosanoids and ADP. These compounds are potent mediators of platelet activation, inflammation and vascular tone. In this paper, the structure of the amine-binding protein (ABP) from Rhodnius prolixus, a vector of the trypanosome that causes Chagas disease, is described. ABP binds the biogenic amines serotonin and norepinephrine with high affinity. A complex with tryptamine shows the presence of a binding site for a single ligand molecule in the central cavity of the β-barrel structure. The cavity contains significant additional volume, suggesting that this protein may have evolved from the related nitrophorin proteins, which bind a much larger heme ligand in the central cavity. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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26. Allelic Gene Structure Variations in Anopheles gambiae Mosquitoes.
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Li, Jun, Ribeiro, Jose M. C., and Guiyun Yan
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- *
BIOLOGICAL variation , *ANOPHELES gambiae , *GENETIC transcription , *GENE expression , *GENETIC regulation , *GENOMES , *GENOMICS - Abstract
Background: Allelic gene structure variations and alternative splicing are responsible for transcript structure variations. More than 75% of human genes have structural isoforms of transcripts, but to date few studies have been conducted to verify the alternative splicing systematically. Methodology/Principal Findings: The present study used expressed sequence tags (ESTs) and EST tagged SNP patterns to examine the transcript structure variations resulting from allelic gene structure variations in the major human malaria vector, Anopheles gambiae. About 80% of 236,004 available A. gambiae ESTs were successfully aligned to A. gambiae reference genomes. More than 2,340 transcript structure variation events were detected. Because the current A. gambiae annotation is incomplete, we re-annotated the A. gambiae genome with an A. gambiae-specific gene model so that the effect of variations on gene coding could be better evaluated. A total of 15,962 genes were predicted. Among them, 3,873 were novel genes and 12,089 were previously identified genes. The gene completion rate improved from 60% to 84%. Based on EST support, 82.5% of gene structures were predicted correctly. In light of the new annotation, we found that ∼78% of transcript structure variations were located within the coding sequence (CDS) regions, and >65% of variations in the CDS regions have the same open-reading-frame. The association between transcript structure isoforms and SNPs indicated that more than 28% of transcript structure variation events were contributed by different gene alleles in A. gambiae. Conclusions/Significance: We successfully expanded the A. gambiae genome annotation. We predicted and analyzed transcript structure variations in A. gambiae and found that allelic gene structure variation plays a major role in transcript diversity in this important human malaria vector. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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27. aeGEPUCI: a database of gene expression in the dengue vector mosquito, Aedes aegypti.
- Author
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Dissanayake, Sumudu N., Ribeiro, Jose M. C., Mei-Hui Wang, Dunn, William A., Guiyun Yan, James, Anthony A., and Marinotti, Osvaldo
- Subjects
- *
AEDES aegypti , *DENGUE , *YELLOW fever , *VIRUSES , *GENOMES , *GENE expression , *MOSQUITOES , *DATA mining - Abstract
Background: Aedes aegypti is the principal vector of dengue and yellow fever viruses. The availability of the sequenced and annotated genome enables genome-wide analyses of gene expression in this mosquito. The large amount of data resulting from these analyses requires efficient cataloguing before it becomes useful as the basis for new insights into gene expression patterns and studies of the underlying molecular mechanisms for generating these patterns. Findings: We provide a publicly-accessible database and data-mining tool, aeGEPUCI, that integrates 1) microarray analyses of sex- and stage-specific gene expression in Ae. aegypti, 2) functional gene annotation, 3) genomic sequence data, and 4) computational sequence analysis tools. The database can be used to identify genes expressed in particular stages and patterns of interest, and to analyze putative cis-regulatory elements (CREs) that may play a role in coordinating these patterns. The database is accessible from the address http://www.aegep.bio. uci.edu. Conclusions: The combination of gene expression, function and sequence data coupled with integrated sequence analysis tools allows for identification of expression patterns and streamlines the development of CRE predictions and experiments to assess how patterns of expression are coordinated at the molecular level. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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28. Cutinase-like proteins of Mycobacterium tuberculosis: characterization of their variable enzymatic functions and active site identification.
- Author
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West, Nicholas P., Chow, Frances M. E., Randall, Elizabeth J., Jing Wu, Jian Chen, Ribeiro, Jose M. C., and Britton, Warwick J.
- Subjects
PROTEINS ,MYCOBACTERIUM tuberculosis ,BINDING sites ,ENZYMES ,LIPASES - Abstract
Discovery and characterization of novel secreted enzymes of Mycobacterium tuberculosis are important for understanding the pathogenesis of one of the most important human bacterial pathogens. The proteome of M. tuberculosis contains over 400 potentially secreted proteins, the majority of which are uncharacterized. A family of seven cutinase-like proteins (CULPs) was identified by bioinformatic analysis, expressed and purified from Escherichia coli, and characterized in terms of their enzymatic activities. These studies revealed a functional diversity of enzyme classes based on differential preferences for substrate chain length. One member, Culp1, exhibited strong esterase activity, 40-fold higher than that of Culp6, which had strong activity as a lipase. Another, Culp4, performed moderately as an esterase and weakly as a lipase. Culp6 lipase activity was optimal above pH 7.0, and fully maintained to pH 8.5. None of the CULP members exhibited cutinase activity. Site-directed mutagenesis of each residue of the putative catalytic triad in Culp6 confirmed that each was essential for activity toward all fatty acid chain lengths of nitrophenyl esters and lipolytic function. Culp1 and Culp2 were present only in culture supernatants of M. tuberculosis, while Culp6, which is putatively essential for mycobacterial growth, was retained in the cell wall, suggesting the proteins play distinct roles in mycobacterial biology. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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29. Brugia malayi Excreted/Secreted Proteins at the Host/Parasite Interface: Stage- and Gender-Specific Proteomic Profiling.
- Author
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Bennuru, Sasisekhar, Semnani, Roshanak, Meng, Zhaojing, Ribeiro, Jose M. C., Veenstra, Timothy D., and Nutman, Thomas B.
- Subjects
PROTEOMICS ,GLUTATHIONE peroxidase ,PROTEINS ,CLONORCHIS sinensis ,DATABASES ,FILARIASIS ,FISH parasites - Abstract
Relatively little is known about the filarial proteins that interact with the human host. Although the filarial genome has recently been completed, protein profiles have been limited to only a few recombinants or purified proteins of interest. Here, we describe a large-scale proteomic analysis using microcapillary reverse-phase liquid chromatography-tandem-mass spectrometry to identify the excretory-secretory (ES) products of the L3, L3 to L4 molting ES, adult male, adult female, and microfilarial stages of the filarial parasite Brugia malayi. The analysis of the ES products from adult male, adult female, microfilariae (Mf), L3, and molting L3 larvae identified 852 proteins. Annotation suggests that the functional and component distribution was very similar across each of the stages studied; however, the Mf contributed a higher proportion to the total number of identified proteins than the other stages. Of the 852 proteins identified in the ES, only 229 had previous confirmatory expressed sequence tags (ESTs) in the available databases. Moreover, this analysis was able to confirm the presence of 274 "hypothetical" proteins inferred from gene prediction algorithms applied to the B. malayi (Bm) genome. Not surprisingly, the majority (160/274) of these "hypothetical" proteins were predicted to be secreted by Signal IP and/or SecretomeP 2.0 analysis. Of major interest is the abundance of previously characterized immunomodulatory proteins such as ES-62 (leucyl aminopeptidase), MIF-1, SERPIN, glutathione peroxidase, and galectin in the ES of microfilariae (and Mf-containing adult females) compared to the adult males. In addition, searching the ES protein spectra against the Wolbachia database resulted in the identification of 90 Wolbachia-specific proteins, most of which were metabolic enzymes that have not been shown to be immunogenic. This proteomic analysis extends our knowledge of the ES and provides insight into the host–parasite interaction. Author Summary: Human lymphatic filariasis caused by the nematode parasites Brugia malayi and Wuchereria bancrofti are a major cause of concern in tropical countries. Studies over several decades have identified various proteins of these parasites that have highlighted their role in host–parasite interactions and possible chemotherapeutic and prophylactic interventions. The availability of the parasite genome facilitates the identification of all of the proteins of the parasite that could interact with the host. In this study, we have attempted to identify the excretory-secretory proteins of the various stages of the parasite that could be maintained in vitro for a limited period utilizing a high-throughput proteomics approach. We observe and report that the parasites expend resources to secrete out various molecules that they utilize to evade the host immune system and modulate its responses. Further, this study also provides information on the predicted hypothetical proteins to be bonafide proteins and thus a catalogue of the excretory-secretory proteins towards a better understanding of the host–parasite interactions. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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- View/download PDF
30. Molecular Evolution of Immune Genes in the Malaria Mosquito Anopheles gambiae.
- Author
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Lehmann, Tovi, Hume, Jen C. C., Licht, Monica, Burns, Christopher S., Wollenberg, Kurt, Simard, Fred, and Ribeiro, Jose M. C.
- Subjects
ANOPHELES gambiae ,HOST-parasite relationships ,MOLECULAR evolution ,IMMUNE system ,PATHOGENIC microorganisms ,MALARIA ,PUBLIC health - Abstract
Background: As pathogens that circumvent the host immune response are favoured by selection, so are host alleles that reduce parasite load. Such evolutionary processes leave their signature on the genes involved. Deciphering modes of selection operating on immune genes might reveal the nature of host-pathogen interactions and factors that govern susceptibility in host populations. Such understanding would have important public health implications. Methodology/Findings: We analyzed polymorphisms in four mosquito immune genes (SP14D1, GNP, defensin, and gambicin) to decipher selection effects, presumably mediated by pathogens. Using samples of Anopheles arabiensis, An. quadriannulatus and four An. gambiae populations, as well as published sequences from other Culicidae, we contrasted patterns of polymorphisms between different functional units of the same gene within and between populations. Our results revealed selection signatures operating on different time scales. At the most recent time scale, within-population diversity revealed purifying selection. Between populations and between species variation revealed reduced differentiation (GNBP and gambicin) at coding vs. noncoding- regions, consistent with balancing selection. McDonald-Kreitman tests between An. quadriannulatus and both sibling species revealed higher fixation rate of synonymous than nonsynonymous substitutions (GNBP) in accordance with frequency dependent balancing selection. At the longest time scale (.100 my), PAML analysis using distant Culicid taxa revealed positive selection at one codon in gambicin. Patterns of genetic variation were independent of exposure to human pathogens. Significance and Conclusions: Purifying selection is the most common form of selection operating on immune genes as it was detected on a contemporary time scale on all genes. Selection for ''hypervariability'' was not detected, but negative balancing selection, detected at a recent evolutionary time scale between sibling species may be rather common. Detection of positive selection at the deepest evolutionary time scale suggests that it occurs infrequently, possibly in association with speciation events. Our results provided no evidence to support the hypothesis that selection was mediated by pathogens that are transmitted to humans. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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31. ROLE OF ARTHROPOD SALIVA IN BLOOD FEEDING: Sialome and Post-Sialome Perspectives.
- Author
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Ribeiro, Jose M. C. and Francischetti, Ivo M. B.
- Subjects
- *
HEMOSTASIS , *INVERTEBRATES , *SALIVA , *NOCICEPTORS , *ENTOMOLOGY - Abstract
This review addresses the problems insects and ticks face to feed on blood and the solutions these invertebrates engender to overcome these obstacles, including a sophisticated salivary cocktail of potent pharmacologic compounds. Recent advances in transcriptome and proteome research allow an unprecedented insight into the complexity of these compounds, indicating that their molecular diversity as well as the diversity of their targets is still larger than previously thought. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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32. Salivary Glands of the Sand Fly Phlebotomus Papatasi Contain Pharmacologically Active Amounts of...
- Author
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Ribeiro, Jose M. C. and Katz, Oren
- Subjects
- *
SAND flies , *PHLEBOTOMUS , *ADENOSINE monophosphate - Abstract
Presents information on a study which demonstrated that the salivary glands of the sand fly Phlebotomus Papatasi contain adenosine monophosphate and 5-AMP. Materials and methods; Results; Discussion.
- Published
- 1999
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33. Purification and Cloning of the Salivary Peroxidase/Catechol Oxidase of the Mosquito Anopheles...
- Author
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Ribeiro, Jose M. C. and Valenzuela, Jesus G.
- Subjects
- *
ANOPHELES , *SALIVARY glands , *PEROXIDASE - Abstract
Focuses on a study which purified the salivary peroxidase of the mosquito Anopheles albimanus. Review of related studies; Materials and methods; Results and discussion.
- Published
- 1999
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34. ANTI-TICK ANTIBODIES: AN EPIDEMIOLOGIC TOOL IN LYME DISEASE RESEARCH.
- Author
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SCHWARTZ, BRIAN S., RIBEIRO, JOSE M. C., and GOLDSTEIN, MICHAEL D.
- Published
- 1990
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35. COMPARING THE RELATIVE POTENTIAL OF RODENTS AS RESERVOIRS OF THE LYME DISEASE SPIROCHETE (BORRELIABURGDORFERI).
- Author
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MATHER, THOMAS N., WILSON, MARK L., MOORE, SEAN I., RIBEIRO, JOSE M. C, and SPIELMAN, ANDREW
- Published
- 1989
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- View/download PDF
36. Effect of ATP analogues on the gorging response of Aedes aegypti.
- Author
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GALUN, RACHEL, KOONTZ, LOUIS C., GWADZ, ROBERT W., and RIBEIRO, JOSE M. C.
- Published
- 1985
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37. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis.
- Author
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Ferreira, Viviana P., Fazito Vale, Vladimir, Pangburn, Michael K., Abdeladhim, Maha, Ferreira Mendes-Sousa, Antonio, Coutinho-Abreu, Iliano V., Rasouli, Manoochehr, Brandt, Elizabeth A., Meneses, Claudio, Lima, Kolyvan Ferreira, Nascimento Araújo, Ricardo, Horácio Pereira, Marcos, Kotsyfakis, Michalis, Oliveira, Fabiano, Kamhawi, Shaden, Ribeiro, Jose M. C., Gontijo, Nelder F., Collin, Nicolas, and Valenzuela, Jesus G.
- Published
- 2016
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38. Reducing Transmission of Lyme Disease Spirochetes in a Suburban Setting.
- Author
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MATHER, THOMAS N., RIBEIRO, JOSE M. C., MOORE, SEAN I., and SPIELMAN, ANDREW
- Published
- 1988
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39. Aspects of the release of superoxide by leukocytes, and a means by which this is switched off
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Ribeiro, Jose M. C., Colepicolo, Pio, Camerero, Valeria C. P. C., Bishop, Amy, Karnovsky, Manfred L., Gallop, Paul M., and Paz, Mercedes A.
- Published
- 1994
40. Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed Aedes aegypti Female and Male Mosquitoes.
- Author
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Alvarenga PH, Alves E Silva TL, Suzuki M, Nardone G, Cecilio P, Vega-Rodriguez J, Ribeiro JMC, and Andersen JF
- Subjects
- Humans, Animals, Male, Female, Sugars metabolism, Hemolymph metabolism, Proteomics, Carbohydrates, Aedes metabolism
- Abstract
In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male Aedes aegypti mosquitoes. A total of 1403 proteins were identified, out of which 447 of them were predicted to be extracellular. In both sexes, almost half of these extracellular proteins were predicted to be involved in defense/immune response, and their relative abundances (based on their intensity-based absolute quantification, iBAQ) were 37.9 and 33.2%, respectively. Interestingly, among them, 102 serine proteases/serine protease-homologues were identified, with almost half of them containing CLIP regulatory domains. Moreover, proteins belonging to families classically described as chemoreceptors, such as odorant-binding proteins (OBPs) and chemosensory proteins (CSPs), were also highly abundant in the hemolymph of both sexes. Our data provide a comprehensive catalogue of A. aegypti hemolymph basal protein content, revealing numerous unexplored targets for future research on mosquito physiology and disease transmission. It also provides a reference for future studies on the effect of blood meal and infection on hemolymph composition.
- Published
- 2024
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- View/download PDF
41. A comparison of Illumina and PacBio methods to build tick salivary gland transcriptomes confirms large expression of lipocalins and other salivary protein families that are not represented in available tick genomes.
- Author
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Guizzo MG, Mans B, Pienaar R, and Ribeiro JMC
- Subjects
- Animals, Transcriptome, Proteome metabolism, Lipocalins genetics, Lipocalins metabolism, Salivary Glands, Salivary Proteins and Peptides genetics, Rhipicephalus genetics, Ixodes genetics
- Abstract
Tick saliva helps blood feeding by its antihemostatic and immunomodulatory activities. Tick salivary gland transcriptomes (sialotranscriptomes) revealed thousands of transcripts coding for putative secreted polypeptides. Hundreds of these transcripts code for groups of similar proteins, constituting protein families, such as the lipocalins and metalloproteases. However, while many of these transcriptome-derived protein sequences matches sequences predicted by tick genome assemblies, the majority are not represented in these proteomes. The diversity of these transcriptome-derived transcripts could derive from artifacts generated during assembly of short Illumina reads or derive from polymorphisms of the genes coding for these proteins. To investigate this discrepancy, we collected salivary glands from blood-feeding ticks and, from the same homogenate, made and sequenced libraries following Illumina and PacBio protocols, with the assumption that the longer PacBio reads would reveal the sequences generated by the assembly of Illumina reads. Using both Rhipicephalus zambeziensis and Ixodes scapularis ticks, we have obtained more lipocalin transcripts from the Illumina library than the PacBio library. To verify whether these unique Illumina transcripts were real, we selected 9 uniquely Illumina-derived lipocalin transcripts from I. scapularis and attempted to obtain PCR products. These were obtained and their sequences confirmed the presence of these transcripts in the I. scapularis salivary homogenate. We further compared the predicted salivary lipocalins and metalloproteases from I. scapularis sialotranscriptomes with those found in the predicted proteomes of 3 publicly available genomes of I. scapularis. Results indicate that the discrepancy between the genome and transcriptome sequences for these salivary protein families is due to a high degree of polymorphism within these genes., (Copyright © 2023. Published by Elsevier GmbH.)
- Published
- 2023
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42. A draft of the genome of the Gulf Coast tick, Amblyomma maculatum.
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Ribeiro JMC, Bayona-Vásquez NJ, Budachetri K, Kumar D, Frederick JC, Tahir F, Faircloth BC, Glenn TC, and Karim S
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- Animals, Male, Amblyomma genetics, Genomics, RNA, Ixodidae genetics, Ixodidae microbiology, Rickettsia genetics, Ticks genetics
- Abstract
The Gulf Coast tick, Amblyomma maculatum, inhabits the Southeastern states of the USA bordering the Gulf of Mexico, Mexico, and other Central and South American countries. More recently, its U.S. range has extended West to Arizona and Northeast to New York state and Connecticut. It is a vector of Rickettsia parkeri and Hepatozoon americanum. This tick species has become a model to study tick/Rickettsia interactions. To increase our knowledge of the basic biology of A. maculatum we report here a draft genome of this tick and an extensive functional classification of its proteome. The DNA from a single male tick was used as a genomic source, and a 10X genomics protocol determined 28,460 scaffolds having equal or more than 10 Kb, totaling 1.98 Gb. The N50 scaffold size was 19,849 Kb. The BRAKER pipeline was used to find the protein-coding gene boundaries on the assembled A. maculatum genome, discovering 237,921 CDS. After trimming and classifying the transposable elements, bacterial contaminants, and truncated genes, a set of 25,702 were annotated and classified as the core gene products. A BUSCO analysis revealed 83.4% complete BUSCOs. A hyperlinked spreadsheet is provided, allowing browsing of the individual gene products and their matches to several databases., (Copyright © 2022. Published by Elsevier GmbH.)
- Published
- 2023
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- View/download PDF
43. An insight into the female and male Sabethes cyaneus mosquito salivary glands transcriptome.
- Author
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Smith LB, Chagas AC, Martin-Martin I, Ribeiro JMC, and Calvo E
- Subjects
- Humans, Male, Female, Animals, Transcriptome, Mosquito Vectors, Salivary Glands metabolism, Anopheles genetics, Anopheles metabolism, Aedes genetics
- Abstract
Mosquitoes are responsible for the death and debilitation of millions of people every year due to the pathogens they can transmit while blood feeding. While a handful of mosquitoes, namely those in the Aedes, Anopheles, and Culex genus, are the dominant vectors, many other species belonging to different genus are also involved in various pathogen cycles. Sabethes cyaneus is one of the many poorly understood mosquito species involved in the sylvatic cycle of Yellow Fever Virus. Here, we report the expression profile differences between male and female of Sa.cyaneus salivary glands (SGs). We find that female Sa.cyaneus SGs have 165 up-regulated and 18 down-regulated genes compared to male SGs. Most of the up-regulated genes have unknown functions, however, odorant binding proteins, such as those in the D7 protein family, and mucins were among the top 30 genes. We also performed various in vitro activity assays of female SGs. In the activity analysis we found that female SG extracts inhibit coagulation by blocking factor Xa and has endonuclease activity. Knowledge about mosquitoes and their physiology are important for understanding how different species differ in their ability to feed on and transmits pathogens to humans. These results provide us with an insight into the Sabethes SG activity and gene expression that expands our understanding of mosquito salivary glands., (Copyright © 2022. Published by Elsevier Ltd.)
- Published
- 2023
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- View/download PDF
44. Rickettsia parkeri infection modulates the sialome and ovariome of the Gulf coast tick, Amblyomma maculatum .
- Author
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Guizzo MG, Budachetri K, Adegoke A, Ribeiro JMC, and Karim S
- Abstract
The Gulf Coast tick, Amblyomma maculatum , is a vector of several tick-borne pathogens, including Rickettsia parkeri . The ability of R . parkeri to persist within the tick population through transovarial and transstadial transmission, without apparently harming the ticks, contributes to the pathogen's perpetuation in the tick population. Previous studies have shown that the R . parkeri load in A . maculatum is regulated by the tick tissues' oxidant/antioxidant balance and the non-pathogenic tick microbiome. To obtain further insights into the interaction between tick and pathogen, we performed a bulk RNA-Seq for differential transcriptomic analysis of ovaries and salivary glands from R . parkeri -infected and uninfected ticks over the feeding course on a host. The most differentially expressed functional category was of bacterial origin, exhibiting a massive overexpression of bacterial transcripts in response to the R . parkeri infection. Candidatus Midichloria mitochondrii and bacteria from the genus Rickettsia were mainly responsible for the overexpression of bacterial transcripts. Host genes were also modulated in R . parkeri -infected tick organs. A similar number of host transcripts from all analyzed functional categories was negatively and positively modulated, revealing a global alteration of the A . maculatum transcriptome in response to pathogen infection. R . parkeri infection led to an increase in salivary transcripts involved in blood feeding success as well as a decrease in ovarian immune transcripts. We hypothesize that these transcriptional alterations facilitate pathogen persistence and transmission within tick population., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Guizzo, Budachetri, Adegoke, Ribeiro and Karim.)
- Published
- 2022
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45. The structures of two salivary proteins from the West Nile vector Culex quinquefasciatus reveal a beta-trefoil fold with putative sugar binding properties.
- Author
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Kern O, Valenzuela Leon PC, Gittis AG, Bonilla B, Cruz P, Chagas AC, Ganesan S, Ribeiro JMC, Garboczi DN, Martin-Martin I, and Calvo E
- Abstract
Female mosquitoes require blood meals for egg development. The saliva of blood feeding arthropods contains biochemically active molecules, whose anti-hemostatic and anti-inflammatory properties facilitate blood feeding on vertebrate hosts. While transcriptomics has presented new opportunities to investigate the diversity of salivary proteins from hematophagous arthropods, many of these proteins remain functionally undescribed. Previous transcriptomic analysis of female salivary glands from Culex quinquefasciatus, an important vector of parasitic and viral infections, uncovered a 12-member family of putatively secreted proteins of unknown function, named the Cysteine and Tryptophan-Rich (CWRC) proteins. Here, we present advances in the characterization of two C. quinquefasciatus CWRC family members, CqDVP-2 and CqDVP-4, including their enrichment in female salivary glands, their specific localization within salivary gland tissues, evidence that these proteins are secreted into the saliva, and their native crystal structures, at 2.3 Å and 1.87 Å, respectively. The β-trefoil fold common to CqDVP-2 and CqDVP-4 is similar to carbohydrate-binding proteins, including the B subunit of the AB toxin, ricin, from the castor bean Ricinus communis . Further, we used a glycan array approach, which identifies carbohydrate ligands associated with inflammatory processes and signal transduction. Glycan array 300 testing identified 100 carbohydrate moieties with positive binding to CqDVP-2, and 77 glycans with positive binding to CqDVP-4. The glycan with the highest relative fluorescence intensities, which exhibited binding to both CqDVP-2 and CqDVP-4, was used for molecular docking experiments. We hypothesize that these proteins bind to carbohydrates on the surface of cells important to host immunology. Given that saliva is deposited into the skin during a mosquito bite, and acts as the vehicle for arbovirus inoculation, understanding the role of these proteins in pathogen transmission is of critical importance. This work presents the first solved crystal structures of C. quinquefasciatus salivary proteins with unknown function. These two molecules are the second and third structures reported from salivary proteins from C. quinquefasciatus , an important, yet understudied disease vector., Competing Interests: There is no conflict of interest.
- Published
- 2021
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46. Molecular mechanisms underlying milk production and viviparity in the cockroach, Diploptera punctata.
- Author
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Jennings EC, Korthauer MW, Hendershot JM, Bailey ST, Weirauch MT, Ribeiro JMC, and Benoit JB
- Subjects
- Animals, Carboxylic Ester Hydrolases metabolism, Cockroaches enzymology, Insect Proteins metabolism, Methyltransferases metabolism, Milk metabolism, RNA Interference, RNA-Seq, Reproduction, Carboxylic Ester Hydrolases genetics, Cockroaches physiology, Gene Expression Regulation, Insect Proteins genetics, Methyltransferases genetics, Transcriptome, Viviparity, Nonmammalian genetics
- Abstract
Viviparous reproduction is characterized by maternal retention of developing offspring within the reproductive tract during gestation, culminating in live birth. In some cases, a mother will provide nutrition beyond that present in the yolk; this is known as matrotrophic viviparity. While this phenomenon is best associated with mammals, it is observed in insects such as the viviparous cockroach, Diploptera punctata. Female D. punctata carry developing embryos in the brood sac, a reproductive organ that acts as both a uterus and a placenta by protecting and providing a nutritive secretion to the intrauterine developing progeny. While the basic physiology of D. punctata pregnancy has been characterized, little is known about the molecular mechanisms underlying this phenomenon. This study combined RNA-seq analysis, RNA interference, and other assays to characterize molecular and physiological changes associated with D. punctata reproduction. A comparison of four stages of the female reproductive cycle and males revealed unique gene expression profiles corresponding to each stage and between sexes. Differentially regulated transcripts of interest include the previously identified family of milk proteins and transcripts associated with juvenile hormone metabolism. RNA interference and methoprene application experiments established the potential impacts of bothbreakdown and synthesis reduction of juvenile hormone in maintaining pregnancy in D. punctata. These studies provide the comprehensive molecular mechanisms associated with cockroach viviparity, which will be a critical resource for comparative purposes among viviparity in insect systems., (Published by Elsevier Ltd.)
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- 2020
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47. Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont.
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Bennuru S, Cotton JA, Ribeiro JM, Grote A, Harsha B, Holroyd N, Mhashilkar A, Molina DM, Randall AZ, Shandling AD, Unnasch TR, Ghedin E, Berriman M, Lustigman S, and Nutman TB
- Subjects
- Animals, Onchocerca volvulus chemistry, Wolbachia chemistry, Onchocerca volvulus genetics, Onchocerca volvulus growth & development, Proteome, Symbiosis, Transcriptome, Wolbachia genetics, Wolbachia growth & development
- Abstract
Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite's adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs., Importance: The global onchocerciasis (river blindness) elimination program will have to rely on the development of new tools (drugs, vaccines, biomarkers) to achieve its goals by 2025. As an adjunct to the completed genomic sequencing of O. volvulus, we used a comprehensive proteomic and transcriptomic profiling strategy to gain a comprehensive understanding of both the vector-derived and human host-derived parasite stages. In so doing, we have identified proteins and pathways that enable novel drug targeting studies and the discovery of novel vaccine candidates, as well as useful biomarkers of active infection., (Copyright © 2016 Bennuru et al.)
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- 2016
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48. The genome of Onchocerca volvulus, agent of river blindness.
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Cotton JA, Bennuru S, Grote A, Harsha B, Tracey A, Beech R, Doyle SR, Dunn M, Hotopp JC, Holroyd N, Kikuchi T, Lambert O, Mhashilkar A, Mutowo P, Nursimulu N, Ribeiro JM, Rogers MB, Stanley E, Swapna LS, Tsai IJ, Unnasch TR, Voronin D, Parkinson J, Nutman TB, Ghedin E, Berriman M, and Lustigman S
- Subjects
- Animals, Genome, Bacterial, Wolbachia genetics, Genome, Helminth, Onchocerca volvulus genetics, Onchocerciasis, Ocular parasitology
- Abstract
Human onchocerciasis is a serious neglected tropical disease caused by the filarial nematode Onchocerca volvulus that can lead to blindness and chronic disability. Control of the disease relies largely on mass administration of a single drug, and the development of new drugs and vaccines depends on a better knowledge of parasite biology. Here, we describe the chromosomes of O. volvulus and its Wolbachia endosymbiont. We provide the highest-quality sequence assembly for any parasitic nematode to date, giving a glimpse into the evolution of filarial parasite chromosomes and proteomes. This resource was used to investigate gene families with key functions that could be potentially exploited as targets for future drugs. Using metabolic reconstruction of the nematode and its endosymbiont, we identified enzymes that are likely to be essential for O. volvulus viability. In addition, we have generated a list of proteins that could be targeted by Federal-Drug-Agency-approved but repurposed drugs, providing starting points for anti-onchocerciasis drug development.
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- 2016
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49. Unique features of a global human ectoparasite identified through sequencing of the bed bug genome.
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Benoit JB, Adelman ZN, Reinhardt K, Dolan A, Poelchau M, Jennings EC, Szuter EM, Hagan RW, Gujar H, Shukla JN, Zhu F, Mohan M, Nelson DR, Rosendale AJ, Derst C, Resnik V, Wernig S, Menegazzi P, Wegener C, Peschel N, Hendershot JM, Blenau W, Predel R, Johnston PR, Ioannidis P, Waterhouse RM, Nauen R, Schorn C, Ott MC, Maiwald F, Johnston JS, Gondhalekar AD, Scharf ME, Peterson BF, Raje KR, Hottel BA, Armisén D, Crumière AJJ, Refki PN, Santos ME, Sghaier E, Viala S, Khila A, Ahn SJ, Childers C, Lee CY, Lin H, Hughes DST, Duncan EJ, Murali SC, Qu J, Dugan S, Lee SL, Chao H, Dinh H, Han Y, Doddapaneni H, Worley KC, Muzny DM, Wheeler D, Panfilio KA, Vargas Jentzsch IM, Vargo EL, Booth W, Friedrich M, Weirauch MT, Anderson MAE, Jones JW, Mittapalli O, Zhao C, Zhou JJ, Evans JD, Attardo GM, Robertson HM, Zdobnov EM, Ribeiro JMC, Gibbs RA, Werren JH, Palli SR, Schal C, and Richards S
- Subjects
- Animals, Genome, Humans, Sequence Analysis, DNA, Bedbugs genetics, Ectoparasitic Infestations, Feeding Behavior, Gene Transfer, Horizontal genetics, Host-Parasite Interactions genetics, Insecticide Resistance genetics, Insecticides
- Abstract
The bed bug, Cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. This global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. Analyses of the C. lectularius sequenced genome (650 Mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory repertoire of genes related to obligate hematophagy, host-symbiont interactions, and several mechanisms of insecticide resistance. In addition, we document the presence of multiple putative lateral gene transfer events. Genome sequencing and annotation establish a solid foundation for future research on mechanisms of insecticide resistance, human-bed bug and symbiont-bed bug associations, and unique features of bed bug biology that contribute to the unprecedented success of C. lectularius as a human ectoparasite.
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- 2016
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50. Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi.
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Jiang X, Peery A, Hall AB, Sharma A, Chen XG, Waterhouse RM, Komissarov A, Riehle MM, Shouche Y, Sharakhova MV, Lawson D, Pakpour N, Arensburger P, Davidson VL, Eiglmeier K, Emrich S, George P, Kennedy RC, Mane SP, Maslen G, Oringanje C, Qi Y, Settlage R, Tojo M, Tubio JM, Unger MF, Wang B, Vernick KD, Ribeiro JM, James AA, Michel K, Riehle MA, Luckhart S, Sharakhov IV, and Tu Z
- Subjects
- Animals, Anopheles metabolism, Chromosome Mapping, Chromosomes, Insect genetics, Cluster Analysis, Evolution, Molecular, Genome, Insect, Humans, Insect Proteins genetics, Insect Proteins metabolism, Malaria transmission, Phylogeny, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Synteny, Transcriptome, Urban Population, Anopheles genetics, Insect Vectors genetics
- Abstract
Background: Anopheles stephensi is the key vector of malaria throughout the Indian subcontinent and Middle East and an emerging model for molecular and genetic studies of mosquito-parasite interactions. The type form of the species is responsible for the majority of urban malaria transmission across its range., Results: Here, we report the genome sequence and annotation of the Indian strain of the type form of An. stephensi. The 221 Mb genome assembly represents more than 92% of the entire genome and was produced using a combination of 454, Illumina, and PacBio sequencing. Physical mapping assigned 62% of the genome onto chromosomes, enabling chromosome-based analysis. Comparisons between An. stephensi and An. gambiae reveal that the rate of gene order reshuffling on the X chromosome was three times higher than that on the autosomes. An. stephensi has more heterochromatin in pericentric regions but less repetitive DNA in chromosome arms than An. gambiae. We also identify a number of Y-chromosome contigs and BACs. Interspersed repeats constitute 7.1% of the assembled genome while LTR retrotransposons alone comprise more than 49% of the Y contigs. RNA-seq analyses provide new insights into mosquito innate immunity, development, and sexual dimorphism., Conclusions: The genome analysis described in this manuscript provides a resource and platform for fundamental and translational research into a major urban malaria vector. Chromosome-based investigations provide unique perspectives on Anopheles chromosome evolution. RNA-seq analysis and studies of immunity genes offer new insights into mosquito biology and mosquito-parasite interactions.
- Published
- 2014
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