1. Whole Genome Sequence of Two Wild-Derived Mus musculus domesticus Inbred Strains, LEWES/EiJ and ZALENDE/EiJ, with Different Diploid Numbers.
- Author
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Morgan AP, Didion JP, Doran AG, Holt JM, McMillan L, Keane TM, and de Villena FP
- Subjects
- Animals, Animals, Wild classification, Female, Genetic Variation, Genomics methods, High-Throughput Nucleotide Sequencing, Male, Mice, Mice, Inbred Strains classification, Phylogeny, Animals, Wild genetics, Diploidy, Genome, Mice, Inbred Strains genetics
- Abstract
Wild-derived mouse inbred strains are becoming increasingly popular for complex traits analysis, evolutionary studies, and systems genetics. Here, we report the whole-genome sequencing of two wild-derived mouse inbred strains, LEWES/EiJ and ZALENDE/EiJ, of Mus musculus domesticus origin. These two inbred strains were selected based on their geographic origin, karyotype, and use in ongoing research. We generated 14× and 18× coverage sequence, respectively, and discovered over 1.1 million novel variants, most of which are private to one of these strains. This report expands the number of wild-derived inbred genomes in the Mus genus from six to eight. The sequence variation can be accessed via an online query tool; variant calls (VCF format) and alignments (BAM format) are available for download from a dedicated ftp site. Finally, the sequencing data have also been stored in a lossless, compressed, and indexed format using the multi-string Burrows-Wheeler transform. All data can be used without restriction., (Copyright © 2016 Morgan et al.)
- Published
- 2016
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