11 results on '"Chaves, Bárbara Aparecida"'
Search Results
2. Microclimate and the vertical stratification of potential bridge vectors of mosquito‑borne viruses captured by nets and ovitraps in a central Amazonian forest bordering Manaus, Brazil
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Hendy, Adam, Valério, Danielle, Fé, Nelson Ferreira, Hernandez-Acosta, Eduardo, Mendonça, Claudia, Andrade, Eloane, Pedrosa, Igor, Costa, Edson Rodrigues, Júnior, José Tenaçol Andes, Assunção, Flamarion Prado, Chaves, Bárbara Aparecida, Scarpassa, Vera Margarete, Gordo, Marcelo, Buenemann, Michaela, de Lacerda, Marcus Vinícius Guimarães, Hanley, Kathryn A., and Vasilakis, Nikos
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- 2021
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3. Does the Presence or a High Titer of Yellow Fever Virus Antibodies Interfere with Pregnancy Outcomes in Women with Zika Virus Infection?
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Piauilino, Isa Cristina Ribeiro, Souza, Raillon Keven dos Santos, Lima, Maurício Teixeira, Rodrigues, Yanka Karolinna Batista, da Silva, Luís Felipe Alho, Gouveia, Ayrton Sena, Neto, Alexandre Vilhena da Silva, Chaves, Bárbara Aparecida, Alecrim, Maria das Graças Costa, de Menezes, Camila Helena Aguiar Bôtto, Castilho, Márcia da Costa, Baia-da-Silva, Djane Clarys, and Espinosa, Flor Ernestina Martinez
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ZIKA virus infections ,YELLOW fever ,PREGNANCY outcomes ,VIRAL antibodies ,PHYTOPLASMAS ,IMMUNOGLOBULINS ,RECURRENT miscarriage - Abstract
Zika virus (ZIKV) and yellow fever virus (YFV) originated in Africa and expanded to the Americas, where both are co-circulated. It is hypothesized that in areas of high circulation and vaccination coverage against YFV, children of pregnant women have a lower risk of microcephaly. We evaluated the presence and titers of antibodies and outcomes in women who had ZIKV infection during pregnancy. Pregnancy outcomes were classified as severe, moderate, and without any important outcome. An outcome was defined as severe if miscarriage, stillbirth, or microcephaly occurred, and moderate if low birth weight and/or preterm delivery occurred. If none of these events were identified, the pregnancy was defined as having no adverse effects. A sample of 172 pregnant women with an acute ZIKV infection confirmed during pregnancy were collected throughout 2016. About 89% (150 of 169) of them presented immunity against YFV, including 100% (09 of 09) of those who had severe outcomes, 84% (16 of 19) of those who had moderate outcomes, and 89% (125 of 141) of those who had non-outcomes. There was no difference between groups regarding the presence of anti-YFV antibodies (p = 0.65) and YFV titers (p = 0.6). We were unable to demonstrate a protective association between the presence or titers of YFV antibodies and protection against serious adverse outcomes from exposure to ZIKV in utero. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Vector Competence to Zika Virus Changes Depending on the Manaus's Region Origin of the Aedes aegypti: A Study of an Endemic Brazilian Amazonian City
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Paz, Andréia da Costa, Chaves, Bárbara Aparecida, Godoy, Raquel Soares Maia, Coelho, Deilane Ferreira, Júnior, Ademir Bentes Vieira, Alencar, Rodrigo Maciel, Alcântara, João Arthur, Felix, Luiza dos Santos, Oliveira, Cinthia Catharina Azevedo, Monteiro, Wuelton Marcelo, Lacerda, Marcus Vinicius Guimarães, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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virology - Abstract
Zika virus (ZIKV) is transmitted to humans by the infectious bite of mosquitoes like Aedes aegypti. After a viremic blood meal, the virus must infect the midgut, disseminate to tissues, and reach the salivary gland to be transmitted to a vertebrate host. Many factors influence the mosquito’s ability to become infected and transmit viruses, such as the mosquito’s genetic diversity, intrinsic antiviral barriers, and midgut microbiota. This study evaluated the patterns of ZIKV infection in Ae. aegypti field populations of a city. The infection rate, disseminated infection rate, viral transmission rate, and transmission efficiency were measured by quantitative PCR at 14 days post-infection. The results showed that all Ae. aegypti populations had individuals susceptible to ZIKV infection and able to transmit the virus. The infection parameters showed the city’s geographical area of origin of the Ae. aegypti influences their vector competence for ZIKV transmission.
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- 2022
5. Detection of Zika Virus in Aedes aegypti and Aedes albopictus Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon.
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Gomes, Erika Oliveira, Sacchetto, Lívia, Teixeira, Maurício, Chaves, Bárbara Aparecida, Hendy, Adam, Mendonça, Claudia, Guimarães, Izabele, Linhares, Ramon, Brito, Daniela, Valério, Danielle, Cordeiro, Jady Shayenne Mota, Neto, Alexandre Vilhena Silva, Sampaio, Vanderson Souza, Scarpassa, Vera Margarete, Buenemann, Michaela, Vasilakis, Nikos, Baia-da-Silva, Djane Clarys, Nogueira, Maurício Lacerda, Mourão, Maria Paula Gomes, and Lacerda, Marcus Vinícius Guimarães
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AEDES aegypti ,ZIKA virus ,AEDES albopictus ,MOSQUITOES ,ZIKA virus infections ,AEDES ,CELL culture - Abstract
Zika virus (ZIKV) is an RNA flavivirus (Flaviviridae family) endemic in tropical and subtropical regions that is transmitted to humans by Aedes (Stegomyia) species mosquitoes. The two main urban vectors of ZIKV are Aedes aegypti and Aedes albopictus, which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female Ae. aegypti (22 specimens) and Ae. albopictus (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) Ae. aegypti and 5/241 (2%) Ae. albopictus pools screened using RT-qPCR were positive for ZIKV. No supernatants from Ae. aegypti were positive for ZIKV (0%), and 15 out of 241 (6.2%) Ae. albopictus pools were positive. In this study, we provide the first-ever evidence of Ae. albopictus naturally infected with ZIKV in the Amazon region. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
6. Vector Competence for Zika Virus Changes Depending on the Aedes aegypti 's Region of Origin in Manaus: A Study of an Endemic Brazilian Amazonian City.
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da Costa Paz, Andréia, Chaves, Bárbara Aparecida, Godoy, Raquel Soares Maia, Coelho, Deilane Ferreira, Vieira Júnior, Ademir Bentes, Alencar, Rodrigo Maciel, Alcântara, João Arthur, Félix, Luiza dos Santos, Oliveira, Cinthia Catharina Azevedo, Monteiro, Wuelton Marcelo, Lacerda, Marcus Vinicius Guimarães, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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ZIKA virus , *AEDES aegypti , *ZIKA virus infections , *VIRAL transmission , *MOSQUITO control , *SALIVARY glands - Abstract
Zika virus (ZIKV) is transmitted to humans by the infectious bite of mosquitoes such as Aedes aegypti. In a city, the population control of mosquitoes is carried out according to alerts generated by different districts via the analysis of the mosquito index. However, we do not know whether, besides mosquito abundance, the susceptibility of mosquitoes could also diverge among districts and thus impact the dissemination and transmission of arboviruses. After a viremic blood meal, the virus must infect the midgut, disseminate to tissues, and reach the salivary gland to be transmitted to a vertebrate host. This study evaluated the patterns of ZIKV infection in the Ae. aegypti field populations of a city. The disseminated infection rate, viral transmission rate, and transmission efficiency were measured using quantitative PCR at 14 days post-infection. The results showed that all Ae. aegypti populations had individuals susceptible to ZIKV infection and able to transmit the virus. The infection parameters showed that the geographical area of origin of the Ae. aegypti influences its vector competence for ZIKV transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
7. Dengue and Zika virus infection patterns vary among Aedes aegypti field populations from Belo Horizonte, a Brazilian endemic city.
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Godoy, Raquel Soares Maia, Felix, Luiza dos Santos, Orfanó, Alessandra da Silva, Chaves, Bárbara Aparecida, Nogueira, Paula Monalisa, Costa, Breno dos Anjos, Soares, Aline Silva, Oliveira, Cinthia Catharina Azevedo, Nacif-Pimenta, Rafael, Silva, Breno Mello, Duarte, Ana Paula, Lacerda, Marcus Vinicius Guimarães de, Monteiro, Wuelton Marcelo, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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DENGUE hemorrhagic fever ,AEDES aegypti ,ZIKA virus infections ,DENGUE viruses ,GENETIC vectors ,VIRUS diseases - Abstract
Dengue virus (DENV) and Zika virus (ZIKV) belong to the same viral family, the Flaviviridae. They cause recurring threats to the public health systems of tropical countries such as Brazil. The primary Brazilian vector of both viruses is the mosquito Aedes aegypti. After the mosquito ingests a blood meal from an infected person, the viruses infect and replicate in the midgut, disseminate to secondary tissues and reach the salivary gland (SG), where they are ready to be transmitted to a vertebrate host. It is thought that the intrinsic discrepancies among mosquitoes could affect their ability to deal with viral infections. This study confirms that the DENV and ZIKV infection patterns of nine Ae. aegypti field populations found in geographically separate health districts of an endemic Brazilian city vary. We analyzed the infection rate, disseminated infection, vector competence, and viral load through quantitative PCR. Mosquitoes were challenged using the membrane-feeding assay technique and were tested seven and fourteen days post-infection (early and late infection phases, respectively). The infection responses varied among the Ae. aegypti populations for both flaviviruses in the two infection phases. There was no similarity between DENV and ZIKV vector competencies or viral loads. According to the results of our study, the risk of viral transmission overtime after infection either increases or remains unaltered in ZIKV infected vectors. However, the risk may increase, decrease, or remain unaltered in DENV-infected vectors depending on the mosquito population. For both flaviviruses, the viral load persisted in the body even until the late infection phase. In contrast to DENV, the ZIKV accumulated in the SG over time in all the mosquito populations. These findings are novel and may help direct the development of control strategies to fight dengue and Zika outbreaks in endemic regions, and provide a warning about the importance of understanding mosquito responses to arboviral infections. Author summary: Dengue and Zika are neglected diseases caused by viruses transmitted to humans by mosquitoes (vector-borne diseases). The primary vector of both diseases is Aedes aegypti, a highly abundant mosquito in tropical countries and adapted to the urban habitat. The viral cycle in the vector starts when the mosquito bites an infected person and acquires the viruses through the blood meal. When the infected blood reaches the mosquito's midgut, the viruses invade the epithelial cells and disseminate in several organs until they reach the salivary glands, enabling viral transmission to the next person. However, the mosquitoes have developed strategies to combat the viral invasion and dissemination in their body, making this journey a challenge to the viruses. Herein, we show that the mosquito responses against dengue and Zika viruses are distinct. In addition, mosquitoes from separate populations of the same city have different abilities to deal with the viruses in both cases, dengue and Zika infections. Our results show the diversity of responses that the mosquitoes may present to viral infections. These findings may better direct disease control strategies to combat dengue and Zika outbreaks in endemic regions. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
8. Dengue Infection Susceptibility of Five Aedes aegypti Populations from Manaus (Brazil) after Challenge with Virus Serotypes 1–4.
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Chaves, Bárbara Aparecida, Godoy, Raquel Soares Maia, Campolina, Thaís Bonifácio, Júnior, Ademir Bentes Vieira, Paz, Andréia da Costa, Vaz, Evelyn Beatriz da Costa, Silva, Breno Mello, Nascimento, Rêgila Mello, Guerra, Maria das Graças Vale Barbosa, Lacerda, Marcus Vinicius Guimarães, Monteiro, Wuelton Marcelo, Secundino, Nágila Francinete Costa, and Pimenta, Paulo Filemon Paolucci
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AEDES aegypti , *DENGUE viruses , *DENGUE , *SEROTYPES , *VIRAL load , *VIRAL variation , *VIRAL transmission , *ARBOVIRUS diseases - Abstract
The successful spread and maintenance of the dengue virus (DENV) in mosquito vectors depends on their viral infection susceptibility, and parameters related to vector competence are the most valuable for measuring the risk of viral transmission by mosquitoes. These parameters may vary according to the viral serotype in circulation and in accordance with the geographic origin of the mosquito population that is being assessed. In this study, we investigated the effect of DENV serotypes (1–4) with regards to the infection susceptibility of five Brazilian Ae. aegypti populations from Manaus, the capital of the state of Amazonas, Brazil. Mosquitoes were challenged by oral infection with the DENV serotypes and then tested for the presence of the arbovirus using quantitative PCR at 14 days post-infection, which is the time point that corresponds to the extrinsic incubation period of Ae. aegypti when reared at 28 °C. Thus, we were able to determine the infection patterns for DENV-1, -2, -3 and -4 in the mosquito populations. The mosquitoes had both interpopulation and inter-serotype variation in their viral susceptibilities. All DENV serotypes showed a similar tendency to accumulate in the body in a greater amount than in the head/salivary gland (head/SG), which does not occur with other flaviviruses. For DENV-1, DENV-3, and DENV-4, the body viral load varied among populations, but the head/SG viral loads were similar. Differently for DENV-2, both body and head/SG viral loads varied among populations. As the lack of phenotypic homogeneity represents one of the most important reasons for the long-term fight against dengue incidence, we expect that this study will help us to understand the dynamics of the infection patterns that are triggered by the distinct serotypes of DENV in mosquitoes. [ABSTRACT FROM AUTHOR]
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- 2022
- Full Text
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9. Lack of Evidence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spillover in Free-Living Neotropical Non-Human Primates, Brazil.
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Sacchetto, Lívia, Chaves, Bárbara Aparecida, Costa, Edson Rodrigues, de Menezes Medeiros, Aline Souza, Gordo, Marcelo, Araújo, Danielle Bastos, Oliveira, Danielle Bruna Leal, da Silva, Ana Paula Betaressi, Negri, Andréia Francesli, Durigon, Edison Luiz, Hanley, Kathryn A., Vasilakis, Nikos, de Lacerda, Marcus Vinícius Guimarães, and Nogueira, Maurício Lacerda
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COVID-19 , *SARS-CoV-2 , *PRIMATES , *DOMESTIC animals , *SERODIAGNOSIS - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent of coronavirus disease 2019 (COVID-19), is responsible for the worst pandemic of the 21st century. Like all human coronaviruses, SARS-CoV-2 originated in a wildlife reservoir, most likely from bats. As SARS-CoV-2 has spread across the globe in humans, it has spilled over to infect a variety of non-human animal species in domestic, farm, and zoo settings. Additionally, a broad range of species, including one neotropical monkey, have proven to be susceptible to experimental infection with SARS-CoV-2. Together, these findings raise the specter of establishment of novel enzootic cycles of SARS-CoV-2. To assess the potential exposure of free-living non-human primates to SARS-CoV-2, we sampled 60 neotropical monkeys living in proximity to Manaus and São José do Rio Preto, two hotspots for COVID-19 in Brazil. Our molecular and serological tests detected no evidence of SAR-CoV-2 infection among these populations. While this result is reassuring, sustained surveillance efforts of wildlife living in close association with human populations is warranted, given the stochastic nature of spillover events and the enormous implications of SARS-CoV-2 spillover for human health. [ABSTRACT FROM AUTHOR]
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- 2021
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10. Natural vertical cotransmission of Dengue virus and Chikungunya virus from Aedes aegypti in Brumado, Bahia, Brazil.
- Author
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Granger Neto HP, Rocha CVS, Correia TML, Silva NMPD, Chaves BA, Secundino NFC, Pimenta PFP, and Melo FF
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- Animals, Brazil, Humans, Mosquito Vectors, Aedes, Chikungunya Fever, Chikungunya virus genetics, Dengue, Dengue Virus genetics, Zika Virus, Zika Virus Infection
- Abstract
Background: Arthropod-borne viruses have recently emerged and are pathogens of various human diseases, including dengue, zika, and chikungunya viruses., Methods: We collectedAedes aegyptilarvae (N = 20) from Brumado, Bahia, Brazil, and treated and individually preserved the specimens. We analyzed the samples for dengue, zika, and chikungunya viruses using molecular biology methods., Results: We found that 25% (N = 5) and 15% (N = 3) were positive exclusively for dengue and chikungunya viruses, respectively; 15% (N = 3) were coinfected with both., Conclusions: This is the first report of dengue and chikungunya virus coinfection in A. aegypti larvae.
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- 2022
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11. Dengue Infection Susceptibility of Five Aedes aegypti Populations from Manaus (Brazil) after Challenge with Virus Serotypes 1-4.
- Author
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Chaves BA, Godoy RSM, Campolina TB, Júnior ABV, Paz ADC, Vaz EBDC, Silva BM, Nascimento RM, Guerra MDGVB, Lacerda MVG, Monteiro WM, Secundino NFC, and Pimenta PFP
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- Animals, Brazil, Dengue Virus genetics, Female, Serogroup, Viral Load, Aedes virology, Dengue Virus physiology, Mosquito Vectors virology
- Abstract
The successful spread and maintenance of the dengue virus (DENV) in mosquito vectors depends on their viral infection susceptibility, and parameters related to vector competence are the most valuable for measuring the risk of viral transmission by mosquitoes. These parameters may vary according to the viral serotype in circulation and in accordance with the geographic origin of the mosquito population that is being assessed. In this study, we investigated the effect of DENV serotypes (1-4) with regards to the infection susceptibility of five Brazilian Ae. aegypti populations from Manaus, the capital of the state of Amazonas, Brazil. Mosquitoes were challenged by oral infection with the DENV serotypes and then tested for the presence of the arbovirus using quantitative PCR at 14 days post-infection, which is the time point that corresponds to the extrinsic incubation period of Ae. aegypti when reared at 28 °C. Thus, we were able to determine the infection patterns for DENV-1, -2, -3 and -4 in the mosquito populations. The mosquitoes had both interpopulation and inter-serotype variation in their viral susceptibilities. All DENV serotypes showed a similar tendency to accumulate in the body in a greater amount than in the head/salivary gland (head/SG), which does not occur with other flaviviruses. For DENV-1, DENV-3, and DENV-4, the body viral load varied among populations, but the head/SG viral loads were similar. Differently for DENV-2, both body and head/SG viral loads varied among populations. As the lack of phenotypic homogeneity represents one of the most important reasons for the long-term fight against dengue incidence, we expect that this study will help us to understand the dynamics of the infection patterns that are triggered by the distinct serotypes of DENV in mosquitoes.
- Published
- 2021
- Full Text
- View/download PDF
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