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Hemerocallis citrina is an edible and medicinal homologous plant, which is rich in many nutrients and bioactive components. Terpenoids are a kind of important bioactive component in H. citrina. To understand the dominant terpenoid metabolites in H. citrina roots, stems, leaves, and flowers, respectively, this study analyzed the terpenoid metabolites in these 4 organs based on the ultra performance liquid chromatography-tandem mass spectrometry technology of widely targeted metabolomics. A total of 43 terpenoids were identified, including 7 kinds of monoterpenoids (16.2%), 18 kinds of ditepenoids (41.9%), 16 kinds of triterpene (37.2% ), and 2 kinds of triterpene saponin (4.7%). Principal component analysis and clustering analysis indicated that the mass spectrometry data were reliable, and the accumulation of terpenoids in different organs was significantly different. The dominant terpenoid metabolites in roots included isopimaradieneone, pimaradienone, abietic acid, scutebarbolide C, and isopimaric acid. The dominant terpenoid metabolites in stems included 3, 9-dihydroxy-13(14)-labden-16,15-olide and 9,19-cyclolanost-24-en-3-ol (cycloartenol). The dominant terpenoid metabolites in leaves included viteagnusin A and ent-3β-acetoxyisopimar-15-8β-ol. The dominant terpenoid metabolites in flowers included vitexilactone and 7-Ocoumaroyl- loganic acid. This study listed the relative contents of all terpenoids in roots, stems, leaves, and flowers of H. citrina in detail, which provided the foundation for further investigation of the different usages of various plant organs of H. citrina, and the information is important for the food industry and medical treatment. [ABSTRACT FROM AUTHOR]

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Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this study, Chrysanthemum indicum var. aromaticum was used as material. We examined the changes of biosynthesis of terpenoid and expression of related genes in C. indicum var. aromaticum following the treatment of different durations (0, 0.5, 1, 2, 4 h) of 400 μw·cm-2 UV-B radiation. The results were as follows: (1) The short time of UV-B radiation could significantly promote the expression of related genes in C. indicum var. aromaticum. Compared with the control, 0.5, 1, 2, 4 h treatment of UV-B radiation increased the expression of related genes. Meanwhile, relative expressions of HMGR, DXR, TPS, GPS reached the peak after two hours' treatment of UV-B radiation. As well as the relative expressions of FPS and DXS reached the maximum at four hours' treatment. A significant increase in FPS gene expression was found when plant exposed to four hours of UV-B duration (69 times compared with control). (2) In mevalonate pathway (MVA), the contents of calamenene, cadinene increased following the change of FPS gene expression during four hours of UV-B radiation. The change of the content of 1-caryophyllene was consistent with the change of HMGR gene expression, which increased first and then decreased. (3) In the pathway of 2-methyl-D-erythritol-4-phosphate (MEP), the contents of α-thujone, thujone and β-thujone showed the same trend as the expressions of DXR, TPS and GPS. The content of cajuputole generally increased during four hours' treatment responding to the change of DXS gene expression. In summary, the data indicate that UV-B radiation can change the regulation of terpenoid biosynthesis in C. indicum var. aromaticum by affecting some key genes' expressions. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Pinus massoniana is one of the most important tree species for afforestation in South China, and it is of great economic value and ecological benefit. This study aimed to reveal the cytological basis in relation to induced resistance during the biosynthesis of terpenoids in P. massoniana, and provide the guide for the further research on molecular mechanism of induced insect resistance in P. massoniana. In the illumination incubator with strictly controlled conditions of light and temperature, variations of microscopic structure, terpenoid synthetase activity and its cytochemical localization of needles were investigated in P. massoniana seedlings treated by exogenous jasmonates. The results showed that there were increases in the relative contents of terpenoids, especially, in those of monoterpenoids and diterpenoids, and the antifeedant of Dendrolimus punctatus against needles in Pinus massoniana seedlings was increased following the treatment of 0.2 mmol·L-1 MeJA, which indicated that the induced resistance of P. massoniana were strengthened. For the variations of anatomical structure, secretions of resin canal were enhanced, the number of chloroplast was reduced, the volume of it was increased, and the thylakoid of it was enhanced in the needles of P. massoniana seedlings. The activity of terpenoid synthetase was increased after four weeks of MeJA treatment. The localization of terpenoid synthetase activity was remarkably increased from membrane system, especially, from chloroplast membrane in the needles of P. massoniana seedlings using the method for electron microscopic cytochemistry of enzyme. This implies that the induced resistance is closely related to the changed chloroplast structure and terpenoid synthetase activity in green plastids in P. massaniana exposed to MeJA treatment. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Fifteen compounds were isolated from the 70% EtOH extract of leaves of Chinese hawthorn(Crataegus pinnatifida var. major) by various purification steps, and their structures were determined as 2α,3α,12β,19α,-tetrahydroxyursan-13β,28-olide(1),euscaphic acid(2), tormentic acid(3), ursolic acid(4), pomolic acid(5), corosolic acid(6), maslinic acid(7), linalyl rutinoside(8),(Z)-3-hexenyl β-D-glucoside(9),(3S, 6S)-cis-linalool-3,7-oxide-β-D-glucopyranoside(10), pisumionoside(11), icariside B6(12), byzantionoside B(13),(6R,7E,9R)-9-Hydroxy-4,7-megastigmadien-3-one 9-O-β-D-glucopyranoside(14) and(6S,7E,9R)-6,9-dihydroxy-4,7-megastigmadien-3-one 9-O-β-D-glucopyranoside(15) mainly based on the mass spectrum(MS) and nuclear magnetic resonance(NMR) spectroscopic techniques, of which compound 1 was a new pentacyclic triterpene, and compounds 2, 5, 6, 8, 10, 13 and 15 were isolated form this plant for the first time.