UV-B辐射对神农香菊萜类物质合成及其相关基因表达的影响.

In this study, Chrysanthemum indicum var. aromaticum was used as material. We examined the changes of biosynthesis of terpenoid and expression of related genes in C. indicum var. aromaticum following the treatment of different durations (0, 0.5, 1, 2, 4 h) of 400 μw·cm-2 UV-B radiation. The results were as follows: (1) The short time of UV-B radiation could significantly promote the expression of related genes in C. indicum var. aromaticum. Compared with the control, 0.5, 1, 2, 4 h treatment of UV-B radiation increased the expression of related genes. Meanwhile, relative expressions of HMGR, DXR, TPS, GPS reached the peak after two hours' treatment of UV-B radiation. As well as the relative expressions of FPS and DXS reached the maximum at four hours' treatment. A significant increase in FPS gene expression was found when plant exposed to four hours of UV-B duration (69 times compared with control). (2) In mevalonate pathway (MVA), the contents of calamenene, cadinene increased following the change of FPS gene expression during four hours of UV-B radiation. The change of the content of 1-caryophyllene was consistent with the change of HMGR gene expression, which increased first and then decreased. (3) In the pathway of 2-methyl-D-erythritol-4-phosphate (MEP), the contents of α-thujone, thujone and β-thujone showed the same trend as the expressions of DXR, TPS and GPS. The content of cajuputole generally increased during four hours' treatment responding to the change of DXS gene expression. In summary, the data indicate that UV-B radiation can change the regulation of terpenoid biosynthesis in C. indicum var. aromaticum by affecting some key genes' expressions. [ABSTRACT FROM AUTHOR]