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This experiment was conducted to assess the effects of replacing fish meal with fermented corn gluten meal (FCGM) in diets on the growth performance, immune response and intestinal digestive function in Penaeus monodon. In this experiment, grade levels of FCGM (4.4%, 8.8%, 13.2%, 17.6%, 22.0%) were added in the basic diet to instead of 14.3%, 28.6%, 42.9%, 57.1% and 71.4% fish meal and six kinds of isonitrogenous and isoenergetic experimental diets were prepared to feed [initial weight (12.00±0.25) g] Penaeus monodon for 8 weeks. The experimental results showed as follows: ① compared with the control group, when fermented corn protein meal replaced 42.8% and less of fish meal, it did not significantly affect the growth performance of Penaeus monodon (P>0.05); when fermented corn protein meal replaced 57.1% of fish meal, it significantly reduced the weight gain rate and specific growth rate of Penaeus monodon (P<0.05), and the feed coefficient was significantly higher when the replacement level reached 71.4% (P<0.05). ② Compared with the control group, fermented corn protein meal did not significantly (P>0.05) affect serum immunity indexes of Penaeus monodon when 42.8% and less of fish meal was replaced by fermented corn protein meal; 57.1% of fish meal was replaced by fermented corn protein meal significantly (P< 0.05) reduced serum peroxidase (POD) and acid phosphatase (ACP) activities of Penaeus monodon, while when the replacement ratio reached 71.4%, ACP, POD, superoxide dismutase (SOD) and lysozyme (LZM) activities were significantly lower (P<0.05) than those of the control group; alkaline phosphatase (AKP) activity did not differ significantly (P>0.05) among the treatment groups. ③ Substitution of 42.8% and less of fishmeal by fermented corn protein powder did not affect the intestinal digestive enzyme activities of Spotted Penaeus monodon compared to the control group (P>0.05), whereas substitution levels of 42.8% and more significantly decreased intestinal protease activity, amylase activity and lipase activity (P<0.05). In conclusion, replacing fish meal with FCGM controlled 42.9% in the diet does not have negative effects on growth performance, immune function and intestinal digestive capacity of Penaeus monodon, but higher substitute levels showed serious adverse effects on Penaeus monodon. [ABSTRACT FROM AUTHOR]

The purpose of this study was to explore the composition of muscle nutrients in different culture models of P. monodon, and to provide a theoretical basis for subsequent industrial finishing and select the appropriate model of P. monodon according to the needs. Farming in low-salt soil ponds (salinity: 8.3‰, referred to as low-salt soil pond group), low-salt intensive pond culture (salinity: 7.8‰, referred to as low-salt intensive pond group) and high-salt intensive pond (salinity: 23.4‰, referred to as high-salt group) ,the P. monodon under three culture modes were tested, and the nutritional differences of the muscles of P. monodon under different cultivation modes were analyzed and compared through the analysis and comparison of muscle nutrients. The results showed that in terms of conventional nutrients, the muscle water content of P. monodon in the high-salt group was significantly lower than that of the low-salt intensive pond group and the low-salt soil pond group (P

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Penaeus monodon was one of the three main cultured prawns in the world, which had the characteristics of fast growth, large individual, strong disease resistance, a wide range of temperature and salt, delicious meat, and high market price. Guangdong, Fujian, Hainan, and other places were the main areas for rearing and breeding of Penaeus monodon in China, and the traditional extensive production method was its main production method. As the model’s shortcomings continue to emerge, this water and soil resource-dependent and ecologically destructive production method had become one of the major problems that restricted the sustainable development of Penaeus monodon. Circulating the aquaculture system was the current development direction of advanced aquaculture productivity, but so far there were still few reports on pond aquaculture and related research. In order to explore the feasibility and application development value of Penaeus monodon recirculating aquaculture, two sets of recirculating aquaculture systems composed of a pond, a protein separation unit, an inner circulating fluidized bed, and a circulating pump were designed and constructed in Rudong Base of Institute of Fishery Machinery and Instrument, Chinese Academy of Fishery Sciences in 2019, and the aquaculture experiments were carried out. In the whole breeding process, the aeration aerator assembly was opened, and the water truck aerator was opened in the later breeding stage. The water circulation treatment system operated in the middle and late stages of aquaculture, and the circulation capacity was 26.50 m3/h (middle stage) and 33.00 m3/h (late stage). The amount of sewage discharged was 4%-10% (middle stage) and 10%-12% (later stage) of the aquaculture water body. In this paper, the key equipment was designed in detail and the circulating capacity was calculated, and the water quality condition, the growth of Penaeus monodon culture, the water purification function of protein separation combined device, internal circulating fluidized bed, and other equipment under the corresponding control mode in different culture stages were studied. Finally, the economy of culture operation was comprehensively analyzed. The results showed that the system operated smoothly for 120 days. During the culturing period, dissolved oxygen concentration was 5.30-7.14 mg/L, the pH value was 7.23-8.44, ammonia nitrogen concentration was 0.43-1.38 mg/L (after stable operation), nitrite nitrogen concentration was 0.15-0.56 mg/L(after stable operation). The results indicated that the removal load of suspended solids was (43.19±7.25) g/m3·h, the removal rate of ammonia nitrogen was (9.95±1.90)%, the dissolved oxygen was (9.45±0.87)%, the removal load of ammonia nitrogen was (122.34±22.56)g/m3·d, and the nitrification ability was better in the later stage than in the middle stage. The Penaeus monodon grew normally in the aquatic ecological environment of recirculating aquaculture mode and went through a rapid growth period, a stable growth period, and a slow growth period successively. The final culture density was 3.02 kg/m2, and the high-efficiency culture result was achieved. The final feed coefficient was 1.67, and the income per square meter of a single crop was 211.40 yuan according to the sales price of 70 yuan/kg. After deducting the cost, the profit per square meter per stubble was 34.78 yuan, the annual profit per square meter was 69.56 yuan based on two crops per year, which had achieved good economic benefits. The results show that the Penaeus monodon circulating aquaculture system offers a water quality control method for its growth characteristics and water environment requirements at different stages, realizes scientific feeding, and provides technical support for the application of the Penaeus monodon circulating aquaculture model. [ABSTRACT FROM AUTHOR]

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Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Crustins are one of the vital antimicrobial peptides in shrimp. CarcininPm3, as one of the members of the crustin family, contained 92 amino acids in its mature peptide. It is a novel antimicrobial peptide in shrimp because of only 45% homology to other species. In this paper, firstly, sequence of carcininPm3 was redesigned according to its mature peptide sequence and codon bias of Escherichia coli (E. coli), and then inserted into pSmartI-SUMO vector to finally obtain pSmartI-SUMO-carcininPm3 reeombinant plasmid. This plasmid was introduced into E. coli BL21 (DE3) and the transformants were capable to express SUMO-carcininPm3 in fusion form when induced by IPTG. Further studies were proceeded to optimize the expression of SUMO-carcininPm3, acquiring reeombinant protein with large quantity and high solubility. Finally, this reeombinant protein was purified by affinity chromatography and the content was up to 195 µg/mL. After treated with SUMO protease, the SUMO tag was removed and tag-free carcininPm.3 peptide was obtained. The above results provid a foundation to the functional analysis and application of carcininPm3. [ABSTRACT FROM AUTHOR]

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)