发酵玉米蛋白粉替代鱼粉对斑节对虾(Penaeus monodon)生长性能、免疫应答和肠道消化酶的影响.

This experiment was conducted to assess the effects of replacing fish meal with fermented corn gluten meal (FCGM) in diets on the growth performance, immune response and intestinal digestive function in Penaeus monodon. In this experiment, grade levels of FCGM (4.4%, 8.8%, 13.2%, 17.6%, 22.0%) were added in the basic diet to instead of 14.3%, 28.6%, 42.9%, 57.1% and 71.4% fish meal and six kinds of isonitrogenous and isoenergetic experimental diets were prepared to feed [initial weight (12.00±0.25) g] Penaeus monodon for 8 weeks. The experimental results showed as follows: ① compared with the control group, when fermented corn protein meal replaced 42.8% and less of fish meal, it did not significantly affect the growth performance of Penaeus monodon (P>0.05); when fermented corn protein meal replaced 57.1% of fish meal, it significantly reduced the weight gain rate and specific growth rate of Penaeus monodon (P<0.05), and the feed coefficient was significantly higher when the replacement level reached 71.4% (P<0.05). ② Compared with the control group, fermented corn protein meal did not significantly (P>0.05) affect serum immunity indexes of Penaeus monodon when 42.8% and less of fish meal was replaced by fermented corn protein meal; 57.1% of fish meal was replaced by fermented corn protein meal significantly (P< 0.05) reduced serum peroxidase (POD) and acid phosphatase (ACP) activities of Penaeus monodon, while when the replacement ratio reached 71.4%, ACP, POD, superoxide dismutase (SOD) and lysozyme (LZM) activities were significantly lower (P<0.05) than those of the control group; alkaline phosphatase (AKP) activity did not differ significantly (P>0.05) among the treatment groups. ③ Substitution of 42.8% and less of fishmeal by fermented corn protein powder did not affect the intestinal digestive enzyme activities of Spotted Penaeus monodon compared to the control group (P>0.05), whereas substitution levels of 42.8% and more significantly decreased intestinal protease activity, amylase activity and lipase activity (P<0.05). In conclusion, replacing fish meal with FCGM controlled 42.9% in the diet does not have negative effects on growth performance, immune function and intestinal digestive capacity of Penaeus monodon, but higher substitute levels showed serious adverse effects on Penaeus monodon. [ABSTRACT FROM AUTHOR]