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A dual-signal fluorescent aptasensor based on gold nanoflower (AuFL) was constructed for the simultaneous detection of ochratoxin A (OTA) and zearalenone (ZEN). Single-stranded DNA containing OTA and ZEN aptamers were covalently cross-linked on the surface of AuFL as a nanocarrier and hybridized with complementary DNA labeled with two fluorescent dyes (FAM and Cy3) to prepare a DNA functionalized nanoprobe. Because of fluorescence resonance energy transfer (FRET) between the fluorescent dyes and AuFL, the nanoprobe itself had no fluorescence. When OTA and ZEN were added, FAM and Cy3 were shed from the probe’s surface, disturbing the FRET process and consequently restoring the fluorescence of both fluorescent dyes. Under optimized conditions, the linear range was from 0.05 to 500 ng/mL, and the detection limit was 0.017 ng/mL for OTA; the linear range was from 0.1 to 500 ng/mL, and the detection limit was 0.033 ng/mL for ZEN. The dual-signal fluorescent aptasensor had good selectivity and reproducibility and was successfully applied to the detection of OTA and ZEN in real wine samples.

Objective To analyze the research status of group psychological training in the field of psychological training using bibliometric methods, predict future research hotspots and development trends, and summarize the shortcomings in group psychological training. Methods Chinese literature related to group psychological training from 2003 to 2022 was retrieved from the China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform (Wanfang), and the VIP Chinese Science and Technology Periodical Database. Qualified literature was selected using NoteExpress V3.9, and the publication volume and journals of the qualified literature were statistically described using Excel. VOSviewer 1.6.18 was used to draw a co-authorship density view of core authors, and CiteSpace 6.1.R6 was used to draw co-occurrence maps, keyword clustering, timeline, and burst analysis for visualization analysis. Results In the 3 major Chinese databases, a total of 4 757 qualified articles were included. The overall publication volume showed a slow growth trend, with medical and educational journals being the main publication outlets. Journal of Chinese Health Psychology got the top in highest number of publications, reaching 171 articles. A total of 173 core authors and representative research teams such as Yang Guoyu, Li Na, Wang Ping and Zhang Yan were identified. Co-occurrence and clustering analysis of keywords showed a focus on empirical intervention research. Keyword burst analysis indicated that themes such as quality of life, psychological state, social functioning, negative emotions, and psychological resilience were the research directions of group psychological training. Group intervention research emerged as a research hotspot, representing the future development trend of psychological training. Conclusion The research hotspots of group psychological training mainly include group psychological counseling, group psychological therapy, group psychological consultation, and group psychological training. They are reflected in areas such as college students, mental health education, positive psychology, anxiety, depression, interpersonal relationships, and psychological qualities, and have obtained certain stage achievemets. However, further exploration and improvement are still needed in theoretical research, research methods, long-term tracking and evaluation, integration of theory and practice, and interdisciplinary cooperation.

Objective To develop a training scheme of psychological training for the developmental group of army sergeants and explore the intervention efficacy of the scheme on their psychological health. Methods Delphi method was used to construct a psychological training program for the developmental group of army sergeants. Eighty army sergeants were recruited as the research subjects, and randomly divided into an experimental group and a control group, with 40 individuals in each group. The experimental group received 10 sessions (90~120 min per session) of group psychological training based on our training program, while the control group received regular psychological health education and political education. The training effects were evaluated using the Self-Rating Depression Scale, Generalized Anxiety Disorder Scale, Psychological Resilience Scale, Perceived Social Support Scale, Brief Coping Style Questionnaire, Perceived Stress Scale, Big Five Personality Inventory, and Self-Evaluation Questionnaire for Group Psychological Training before and in 1 week after the training. Results ① Our training program consisted of 3 units and 8 themes. The first unit focused on self-development, including self-awareness, self-acceptance, and self-growth. The second unit focused on emotion and stress management, including emotion regulation and stress management. The third unit focused on social development, including interpersonal communication, relationship adjustment, and career planning. ② After the training, the experimental group had significantly decreased scores of PHQ and GAD (P < 0.05), and the PHQ score was obviously lower than that of the control group (P < 0.05). ③ The total score of psychological resilience, as well as the sub-scales of toughness, strength, and optimism, significantly increased in the experimental group (P < 0.05), and were significantly higher than those of the control group (P < 0.05). ④ After the training, the dimension of friend support in social support was significantly increased in the experimental group (P < 0.05) and it was notably higher than that of the control group (P < 0.05). ⑤ The positive coping score was significantly increased in the experimental group after the training (P < 0.05). ⑥ The extraversion dimension of the Big Five Personality Inventory was significantly increased in the experimental group after the training (P < 0.05). Conclusion Our training scheme for army sergeants can effectively alleviate anxiety and depression, increase positive coping styles, enhance psychological resilience, friend support in social support, and extraversion in the Big Five Personality.

Objective To investigate the association between retinal nerve fiber layer (RNFL) thickness and cognitive dysfunction in patients with mild ischemic stroke. Methods Total 146 patients with mild ischemic stroke that diagnosed and treated in Chengde Central Hospital in Hebei from January 2020 to December 2021 were included. Optical coherence tomography (OCT) was used to measure the RNFL thickness in each quadrant of both eyes, and Mini⁃Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) were used to evaluate cognitive function. Results In patients with mild ischemic stroke, the RNFL thickness at the superior side of left eye was greater than right eye (t = ⁃ 4.589, P = 0.000), and the RNFL thickness at the temporal side of right eye was greater than left eye (t = 3.639, P = 0.000). Correlation analysis showed that the correlation between the RNFL thickness at the superior side of the left eye and the MMSE score (r = 0.385, P = 0.000), the RNFL thickness at the temporal side of the left eye and the National Institutes of Health Stroke Scale (NIHSS) score at admission (r = 0.170, P = 0.020) were positively correlated. The correlation between the RNFL thickness at the inferior side of the left eye and the history of drinking (r = ⁃ 0.216, P = 0.011), the RNFL thickness at the temporal side of the right eye and the history of hypertension (r = ⁃ 0.194, P = 0.023) were negativley correlated. Conclusions The thinning of RNFL in patients with mild ischemic stroke may have a certain degree of association with cognitive dysfunction.

Predicting cracking radius is a prerequisite for determining the holes spacing of gas extraction technology by liquid CO2 phase transition cracking and permeability improvement, which directly affects the gas extraction effect. Most existing prediction methods are based on single factor analysis. In order to grasp the influence of multiple factors on the radius of liquid CO2 phase transition cracking and effectively predict the spacing between holes, ANSYS/LS-DYNA numerical simulation software is used to carry out the research on predicting the radius of coal seam liquid CO2 phase transition cracking combing with orthogonal experiments. The numerical simulation results indicate that the order of factors affecting the radius of liquid CO2 phase transition cracking is ground stress>gas pressure>coal solidity coefficient. The cracking radius decreases with the increase of stress, and increases with the increase of gas pressure and coal solidity coefficient with a linear relationship. A multiple regression analysis is conducted on the numerical simulation results. A prediction model for the radius of liquid CO2 phase transition cracking is established based on three different coupling conditions of ground stress, gas pressure, and coal solidity coefficient. Industrial experiments are conducted on the coal mine site. Extraction boreholes are set up based on the predicted model calculation results. The pressure index method is used to test and analyze the gas extraction effect. The results show the following points. The gas pressure in the observation holes on both sides of the liquid CO2 phase transition cracking hole shows a decreasing trend with time. The farther away from the cracking hole in the initial stage of extraction, the greater the gas pressure. It is consistent with theoretical analysis and numerical simulation results. The effective cracking range of liquid CO2 phase transition is basically consistent with the predicted results. The gas volume fraction in the observation hole is 73.4% higher than that in the natural extraction hole, and the gas extraction efficiency is significantly improved.

Objective To summarize the clinical manifestations, pathological features and gene mutation diversity of Blau syndrome/early-onset sarcoidosis. Methods We collected general data, clinical manifestations, and auxiliary examination results from 8 patients who were diagnosed of Blau syndrome/early-onset sarcoidosis and treated in our hospital from January 2011 to December 2022, and then summarized and analyzed their characteristics and diversity. Results Among the 8 patients, 4 were males and 4 were females. The onset age was 3 to 8 months old. Rash was the first symptom in 7 patients(87.5%). 6 patients(75.0%) had papules and erythema.3 cases(37.5%) had arthritis. 2 cases(25.0%) had uveitis and other eye inflammation. 4 cases (50.0%) also showed intermittent fever. 3 cases (37.5%) showed symptoms in nerve and respiratory system, and hypertension respectively. The skin histopathology of 8 patients showed non-caseous granuloma formation. In laboratory detection, CRP and TNF-α were significantly increased before treatment, while IL-6, IL-8, TNF-α and IL-2 receptor(IL-2R) were significantly decreased in 5 patients after glucocorticoid therapy. The results of genetic testing showed that 4 of the 7 patients had p.R334W(c.1000C > T) mutation, 1 had p.H313R(c.938A > G) and p.R471C(c.1411C > T)double mutation, and 1 had p.476_477del (c.1427_1429delcct). Conclusions Blau syndrome/early-onset sarcoidosis has significant features in clinical manifestations, histopathology and gene mutation, but it also has diversity.

The cable-driven parallel robot is a special flexible mechanism in parallel mechanism, the end-effector of which is driven by cables instead of rigid rods. It integrates the advantages of flexible robots and parallel robots. A three-degree-of-freedom (3-DOF) under-constrained parallel robot with variable cable mast heights is investigated in this study. The inverse kinematics model of the robot is established by using the vector closed-loop principle. The forward kinematics model of the robot is derived according to cable lengths. The dynamics model of the robot is established based on the Lagrange formula. The static equilibrium workspace (SEW) of the robot is studied using the Monte-Carlo method. Given the motion trajectory of the end-effector, the expected three cable lengths are obtained through the inverse kinematics model of the robot. Taking the expected three cable lengths as the experimental input, the experimental curves of cable lengths and cable tensions as well as the forward kinematics trajectory of the robot are obtained. The experimental results validate the correctness of the forward and inverse kinematics models and the dynamics model of the robot, as well as the effectiveness of the SEW.

Background Thrombotic events are a major complication of hypertrophic cardiomyopathy (HCM). There are only two available risk prediction models for thrombotic events, HCM Risk-CVA score and French HCM score developed by foreign scholars, yet the former one has been found to have limited predictive value in Chinese HCM patients. Objective To develop a risk prediction model for thrombotic events in Chinese patients with HCM. Methods A retrospective cohort study design was used. Five hundred and thirty-seven HCM patients who admitted to West China Hospital of Sichuan University from 2010 to 2018 were recruited. Post-discharge health status was collected by use of telephone follow-up or checking the treatment status recorded in the electronic medical record system once every 6 to 12 months until a composite thrombotic event (defined as the endpoint event) or death occurred or the determined thrombotic risk assessment day of this study (2019-12-31). Univariate and multvariate Cox regression analyses were applied to build a thrombotic risk prediction model, and its internal validation was tested in a resample using the bootstrapping technique. Results Due to data missing, 24 cases were excluded, and the other 513 cases were finally included. During a median follow-up of 4.2 years (IQR: 1.3-6.2 years), thrombotic events occurred in 42 cases (8.18%), with an annual morbidity rate of 2.10%〔95%CI (1.47%, 2.73%) 〕. By multivariate Cox regression analysis, age, prior thrombotic event and left ventricular ejection fraction (LVEF) were identified (P

Aerosol microorganisms are important constituents of aerosols. They participate in physical and chemical reactions in the air and are also closely related to disease transmission and human health. With the SARS-CoV-2 pandemic， aerosol microorganisms have become a public health topic of great concern. Studying the composition and influencing factors of the air microbiome therefore has significant public health implications. Due to the limitations of traditional technologies for sampling and determination， the aerosol microbiome has not been fully understood. However， with the development and maturity of high-throughput sequencing technology， the aerosol microbiome has shown promising research prospects. This article reviews the composition， characteristics， detection methods and influencing factors of aerosol microorganisms， providing basic knowledge for further research on the air microbiome. In-depth research on microbial aerosols has significant implications on urban air quality control， national security and public health.

In this paper, the intergenerational development, component characteristics, and strengthening mechanism of carburized gear materials for aerospace power transmission systems are reviewed. The first-generation carburized gear steel is low-carbon medium/low-alloy steel, and the surface hardening of the carburized layer structure is realized by Fe3C carbides. Due to the low content of alloying elements, the tempering resistance of the first-generation carburized gear steel is poor, and the general service temperature range is within 200 ℃. Among the first-generation carburized gear steels, the content of carbide-formation elements of 16Cr3NiWMoVNbE material is relatively high. This material can be developed for the second-generation carburized gear steel with a wide-range service temperature through the critical saturation carburization process. The second-generation carburized gear steel is a low-carbon medium/high-alloy steel. Some M2C strengthening phases with high tempering resistance can be precipitated during the tempering process by further improving the alloying degree and appropriately increasing the content of Mo element with strong tempering resistance. The overall service temperature range is raised to 350 ℃. The third-generation carburized gear steel is low-carbon ultra-high alloy steel, and the “secondary hardening” effect works thoroughly via computational methods. Therefore, the material can be used in a temperature range below 500 ℃ for a long time. The existing alloy structural steel system can not avoid the problem of rapid strength decay after long-term service at high temperatures above 500 ℃. Therefore, the next-generation carburized gear materials will be developed based on iron-based alloys with excellent oxidation resistance.

As an important radioactive tracer, 14C has been widely used in many fields, such as biomedicine, agriculture, geology, environment etc, due to its ability to reveal the distribution, metabolic mechanism and migration path of the substances. In recent years, the demand for 14C-labeled compounds has continued to grow, and its application field has been expanded. This paper summarizes the sources, preparation, separation and purification methods of 14C isotopes. Meanwhile, this paper reviews the preparation, structural identification and application in related research fields of 14C labeled compounds, and emphatically discussed the development status and trend of 14C isotope separation and purification technology. It is hoped that recyclable utilization of 14C resources can be realized with solving the pollution of 14C emissions, which will further promote the development of 14C application.

The cable-driven parallel robot is a special type of parallel robots whose end-effector is driven by cables instead of rigid rods. A three-degree-of-freedom (3-DOF) under-constrained parallel robot with variable cable mast angle is studied. The inverse kinematics model of the robot is established by using the vector closed-loop principle. According to the Lagrange equation, the dynamics model of the robot is established. Based on the robot PD control theory of model compensation, a PD controller of the robot is designed. Given a motion trajectory of the end-effector of the robot, the pose change of the end-effector is transformed into cable length change through the inverse kinematics of the robot. The cable length is used as the input of the control system. The experimental results verify the correctness of the inverse kinematics and dynamics model of the robot and the effectiveness of the control method.

【Objective】 Yilong Lake is located in Yunnan province. Like many other regions in China, its land usage has undergone change over the past decades. The purpose of this paper is to quantify this change using satellite imageries. 【Method】 The analysis is based on satellite imageries acquired from 1999 to 2018 from the basin. Land use transfer matrix and the CA-Markov model were used to analyze the spatial distribution and change in land usage during this period. 【Result】 ①The land use was dominated by croplands from 1999 to 2005 and forest from 2010 to 2018. ②The areas of cropland, pasture, natural land and surface water bodies had been in decline from 1999 to 2018, with their overall areas reduced by 50.60, 27.17, 24.72 and 6.66 km2, respectively. In contrast, forest and urban areas had both increased significantly. The increase in forest areas is mainly due to the conversion of cropland, pasture and natural lands, while the increase in urban areas is mainly due to the use of cropland and natural land. ③It is expected that by 2050, urban and forest areas in the region will increase to 98.62 and 161.03 km2, respectively, while the area of surface water will decrease. 【Conclusion】 The important goal in land resource management in the Yilong lake watershed in the future should be to control the increase in urban area and the decrease in surface water bodies.

In order to meet the needs of cross-border railway transportation in Europe and improve the efficiency of cross-border railway transportation, cross-border transportation trains must not only be compatible with the railway signaling systems of various countries, but also support the changing of different traction power supply systems. In this regard, the European Train Control System (ETCS) has designed the changing process of traction systems, but there is no research on the backup operation of automatic changing. Through in-depth study on the changing information of traction system of the relevant interfaces, this paper improves the communication protocol between ETCS and TCMS (train control and management system) and the display characteristic of driver-machine interface (DMI), and optimizes the control logic of ETCS changing traction system. A scheme of ETCS changing traction system with manual changing as the backup mode is proposed. This scheme can effectively improve changing efficiency and safety of multi-system locomotive traction system equipped with ETCS system, and the ETCS system using this scheme has been successfully applied to many on-board signal systems in Czech, Hungary and other countries. At the same time, this scheme has reference significance for the research and development of regional railway signalling system in China.

Objective To summarize the clinical, imaging and genetic characteristics of spinocerebellar ataxia 2 (SCA2) families presenting as Parkinsonism. Methods and Results Two families with autosomal dominant SCA2 with Parkinson's syndrome diagnosed by genetic test in Peking University First Hospital from May 2019 to December 2020 were included. The probands in 2 families manifested as lead⁃pipe like rigidity and cerebellum ataxia. Electronystagmography (ENG) in proband of family 1 showed gaze nystagmus, understable saccade, decreased smooth tracking ability and disappeared optokinetic nystagmus, proband of family 2 showed slow saccade, decreased smooth tracking ability and decreased optokinetic nystagmus. Head MRI in both probands of 2 families showed slightly enlarged third ventricle, while the proband of family 2 showed atrophy in brain stem and cerebellar. Whole exome sequencing (WES) in 2 probands showed no gene mutations, Sanger sequencing showed dynamic mutation of ATXN2 gene and the copy number of cytosine⁃adenine⁃guanine (CAG) with 37 repeats. Two probands were finally diagnosed as SCA2, and two pedigrees were diagnosed as SCA2 pedigrees. Conclusions SCA2 can present as Parkinsonism, which is easily to be misdiagnosed as Parkinson's disease due to the effective treatment of levodopa. ENG is helpful in detecting subclinical cerebellar lesions. For Parkinsonism patients with family history, especially when cerebellar lesions are involved, ATXN2 gene testing is recommended.

Objective To investigate the clinical utility of ultrasound parameters in diagnosing neonatal annular pancreas. Methods A total of 56 neonates with descending duodenal obstruction underwent saline-contrast ultrasound examination. Ultrasonic parameters, including dilated duodenum diameter (DDD), dilated duodenum thickness (DDT), and stenosis duodenum diameter (SDD), were measured. The cases were categorized into three groups: annular pancreas (group A, n = 28), descending duodenal atresia (group B, n = 3), and descending duodenal septum (group C, n = 25). One-way ANOVA was employed to compare statistical differences among the three ultrasonic parameters across the groups, followed by LSD-t test for pairwise comparisons between two groups showing significant differences. Receiver operating characteristics analysis was conducted to calculate the area under the curve and determine the cut-off value if there were statistically significant differences observed. Results In group A, group B, group C, the diameters of DDD were measured as(23.2 ± 2.4)mm, (25 ± 1.0)mm, (19.4 ± 2.6) mm, respectively. When comparing these three groups, there was a statistically significant difference between group A or group B and group C in terms of DDD diameter measurements (t = 5.7, P < 0.05; t = 3.793, P < 0.05). However, no statistically significant difference was observed between group A and group B for DDD diameter measurements (t = 1.232, P > 0.05). The corresponding values for DDT were measured as (3.0 ± 0.6)mm, (3.2 ± 0.4) mm, and (2.4 ± 0.3) mm, respectively. When comparing these three groups, the results showed a statistically significant difference between either Group A or Group B compared to Group C in terms of DDT measurement (t = 4.695, P < 0.05; t = 2.778, P < 0.05). However, no statistically significant difference was found between Group A and Group B regard- ing the measurement of DDT (t = 0.666, P > 0.05). The SDD measurements in the three groups were (1.9 ± 0.3) mm, (3.6 ± 0.8)mm, (5.5 ± 0.7)mm, respectively. Statistical analysis using LSD-t test revealed significant differences between the three groups (t = 5.521, P < 0.05; t = 6.142, P < 0.05; t = 25.828, P < 0.05).The sensitivity, specificity, positive predictive value, and negative predictive value for detecting annular pancreas at a cutoff of 2.3 mm were determined to be 96.4%, 100%, 100%, and 96.6%, respectively, with an area under the receiver operating characteristic curve of 0.999. Conclusion SDD could be employed for the diagnosis of neonatal annular pancreas. [ABSTRACT FROM AUTHOR]

Common unsupervised feature selection methods only consider the selection of discriminative features,while ignoring the redundancy of features and failing to consider the problem of small classes,which affect the classification performance.Based on this background,a robust uncorrelated regression algorithm is proposed.First,research on uncorrelated regression,use uncorrelated orthogonal constraints to find irrelevant but discriminative features.Uncorrelated constraints keep the data structure in the Stiefel manifold,making the model have a closed solution,avoiding the possible trivial solutions caused by the traditional ridge regression model.Secondly,the loss function and the regularization term use the L2,1 norm to ensure the robustness of the model and obtain a sparse projection matrix.At the same time,the small class problem is taken into account,so that the number of projection matrices is not limited by the number of classes,and the result is enough projection matrices to improve the classification performance of the model.Theoretical analysis and experimental results on multiple data sets show that the proposed method has better performance than other feature selection methods.

Objective To investigate the effects of X-ray radiation on H9c2 cells and histone acetylation patterns, and to analyze the correlation between them. Methods H9c2 cells were randomly divided into control group and different model groups. The cells of model groups were irradiated with different doses of 2,4,6,8 Gy of X-ray radiation respectively. The activity of LDH in supernatants, the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in cells were detected by chromatometry. Cell apoptotic ratio was measured by annexin V-FITC/PI staining and flow cytometry analysis. Apoptosis-related protein Bcl-2,caspase-3 and histone acetylation related protein H3K9ac,HDAC3 were detected by Western blot. Results Compared with control group, the relative activity of LDH in supernatants and relative content of malondialdehyde (MDA) in H9c2 cells were increased (P＜0.05) in model groups, the relative activity of intracellular superoxide dismutase (SOD) was decreased(P＜0.05) in model groups. The apoptotic rate of H9c2 cells was increased in model groups compared with control group(P＜0.05). The protein expression of caspase-3 increased (P＜0.05), the expression of Bcl-2 decreased in model groups(P＜0.05), and also found that the protein expression of H3K9ac significantly decreased and the expression of HDAC3 significantly increased in model groups(P＜0.05). Conclusions X-ray radiation can induce H9c2 cells' injury and apoptosis,accompanied by the decrease of intracellular histone acetylation level, this change may be related to the increase of HDAC3 expression.

Five monolayer plasmid DNA films were irradiated by low-energy electrons (LEEs) (1~20 eV) in ultra-high vacuum. Crosslinks, single-strand breaks, double-strand breaks and the loss of the supercoiled configuration were analyzed via agarose gel electrophoresis. Base damages were revealed by Escherichia coli base excision and repair endonucleases (Nth and Fpg). The electron energy dependence for the effective yields of various DNA damages showed that no clustered damage within 20 base pairs could be caused by electrons

BackgroundSynchrotron radiation confocal micro X-ray fluorescence (confocal μ-SRXRF) micro-probe is a three dimensional analytical method for detecting chemical elements with depth resolution. It can be used to obtain the chemical element information at different depths of samples in situ, which is suitable in the fields of life and environmental sciences.PurposeThis study aims to apply the confocal μ-SRXRF device and in-situ cryogenic device, developed at the hard X-ray micro-focusing beamline (BL15U1) of Shanghai Synchrotron Radiation Facility (SSRF), to analyze the in vivo chemical elemental imaging of biological samples.MethodsConfocal μ-SRXRF was based on K-B mirrors and polycapillary X-ray optics. The foci of the polycapillary X-ray optics and the incident X-ray beam were coincident by continuous scanning and position modification. The confocal μ-SRXRF was used for in-situ XRF imaging analysis of Arabidopsis thaliana seeds and Daphnia magna, and compared with that of conventional μ-SRXRF. The confocal volume was submerged at different depths within the samples to obtain the depth-dependent information. In addition, an in-situ cryogenic device was used to keep Daphnia magna under the cryogenic environment.ResultsThe spatial distributions of chemical elements for in vivo biological samples were directly obtained by confocal μ-SRXRF, without slicing or drying. The specific distribution of chemical elements in plant tissues or organs is more accurately obtained while the absorption and distribution characteristics of chemical elements in organisms are observed more carefully.ConclusionsConfocal μ-SRXRF can be used to directly and effectively analyze the spatial distribution of chemical elements within biological samples. Meanwhile, it can be expanded to analyze the heat-sensitive biological samples and chemical elements by equipping with in-situ cryogenic device, providing a powerful in-situ characterization technique for the life and environment research.

Design tensile tests, fatigue tests, and reinforcement fatigue tests for EPS insulation boards, and study the effects of bonding rate and bonding method on the tensile bearing capacity and fatigue performance of EPS boards and polyurethane adhesive reinforced specimens. The results show that under the same conditions, except for full adhesion, the average tensile bearing capacity of EPS board is as followsrstrip adhesion>point adhesion>cross adhesion>point frame adhesion, from high to low. The fatigue number of EPS boards is positively correlated with the bonding rate, and the strip bonding method has the best fatigue performance. The average tensile bearing capacity of the specimens reinforced with polyurethane adhesive meets the standard requirement and reaches at least 90% of the full adhesive tensile bearing capacity. The maximum impact of initial fatigue on the reinforcement effect is only 7%, and the fatigue times of point bonding, strip bonding, cross bonding, and point frame bonding after reinforcement reach at least 87%, 89%, 87%, and 75% of full bonding, respectively. The cumulative fatigue times after polyurethane adhesive reinforcement are more, and the overall service life of the EPS board is extended, the reinforcement method is relatively reliable. [ABSTRACT FROM AUTHOR]

A new type of anti-car-running barrier is designed, it adopts the driving mechanism of taking off and rotating at the same time. The whole operation does not need to break the track, and realizes the ground taking off and landing and is suitable for the compound roadway. The change of interception stress and wire rope reaction force is analyzed by theoretical calculation and numerical simulation, and the design weaknesses is adjusted and improved from the stress analysis. Moreover, the field impact test is carried out for the designed products. The results show that the strength of the joint of two railings directly affects the interception effect. The product design is reasonable and has passed the field collision test of national mine product safety mark center.

Reflection in the image not only significantly reduces the image quality, but also seriously affects the subsequent computer vision tasks. So proposed a single image reflection removal cascaded algorithm using non local correlation and contrast constraint. This algorithm utilizes a dual-branch approach for LSTM-based information propagation across cascades. It employs reflection and background features to complement each other and iteratively refine prediction accuracy, ensuring mutual enhancement of the two branches' prediction results. To facilitate training for multiple cascade steps, a positivenegative contrastive regularization loss is introduced. This loss treats background images and original images' features as positive and negative samples, respectively. This ensures that the target image is brought closer to the background image while moving away from the original image in the representation space, narrowing the prediction range and effectively alleviating the ill-posed problem. Additionally, an efficient, low-computational-cost non-local correlation prediction module is proposed, capable of capturing contextual information for all pixels along cross paths. Through further cascade operations, each pixel captures long-distance dependencies across the entire image, enabling the use of surrounding point information to predict background information obscured by strong reflections. Experimental results demonstrate that, compared to current algorithms, the proposed algorithm achieves superior results and exhibits robust performance. [ABSTRACT FROM AUTHOR]

Objective To study the change of STAT1 expression in the anterior cingulate cortex on rats under orth⁃ odontic force, and to further explore the roles of STAT1 and related JAK⁃STAT1 signaling pathway in the mediation and regulation of pain during tooth movement. Methods 112 male Sprague⁃Dawley (SD) rats (225±25 g) were used in this study. They were randomly divided into experimental groups (96 rats) and control groups (16 rats). All rats were in⁃ stalled bilateral maxillary device for tooth movement models. Rats in the experimental groups were applied 80g orth⁃ odontic force on both sides and were divided into six subgroup 4 h, 12 h, 24 h, 2 d, 3 d, 7 d, with 16 rabbits in each subgroup. The control groups were only installed the same orthodontic devices, without the application of orthodontic force. Brain tissue of the anterior cingulate cortex was isolated after 4 h, 12 h, 24 h, 2 d, 3 d, 7 d since experiment, and the expression level of STAT1 and p⁃STAT1 was analyzed with the method of immunofluorescence and PCR. Results For the immunofluorescence result, there was significant difference in STAT1 expression between control groups and dif⁃ ferent experimental groups at different time points in total (P < 0.05). The STAT1 expression amount in the 4 h group decreased significantly when compared with the control group (P < 0.05); to the 2 d group, the difference is still statisti⁃ cally significant (P < 0.01). 3 d group, 7 d group and control group had no statistically significant difference. The STAT1 expression amount in 4 h group, 12 h group, 24 h group was significantly lower than that in 3 d and 7 d groups, differences were statistically significant (P < 0.05). The STAT1 expression in the 2 d group was significantly lower than that of 7 d (42.35 ± 5.77) group, the difference was statistically significant (P < 0.05). There was significant difference in p⁃STAT1 expression between control groups and different experimental groups at different time points in total (F = 623.518, P < 0.05). The p⁃STAT1 expression amount in experimental groups were higher than that in the control group (P < 0.05). The p⁃STAT1 expression in 4 h group was lower than that in 12 h and 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). The p⁃STAT1 expression in 12 h group was lower than that in 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). For the PCR result, the expression of mRNA in STAT1 of experimental groups of 4 h, 12 h, 24 h, 2 d, 3 d, 7 d and the control groups were not statistically significant (P > 0.05). Conclusions After apply⁃ ing orthodontic force, the expression of STAT1 decreased transiently and the expression of p⁃STAT1 increased transient⁃ ly. The reduction of STAT1 was probably caused by the phosphorylation of STAT1 and decrease in the translation level of STAT1, rather than changes in the transcriptional levels. The orthodontic pain might be related with the activation of STAT1 into phosphorylated STAT1.

The quick color change system can complete color change in a short time while saving paint and solvent. The equipment configuration and workflow of quick color change system were introduced, and some issues needing attention during its application were summarized. The future of quick color change application was prospected. It will become mainstream in paint shop to meet the demand of color personalization for automobiles. [ABSTRACT FROM AUTHOR]

The X-ray Energy Dispersive Spectrometry EDS-Mapping technique can be used to directly observe distribution characteristic of elements in rock sections. The distribution characteristics and elemental assemblage of tellurium(Te) and some other elements(Au and Ag) from the Guilaizhuang gold field in Shandong Province of China were investigated by high resolution Scanning Electron Microscope(SEM) and X-ray Energy Dispersive Spectroscope(EDS) Mapping techniques and are presented in this paper. The morphological characteristics of possible Te minerals, as well as the spatial relationship between Te minerals and other minerals, were also qualitatively determined. The results show that the Te-bearing minerals in the Zhuojiazhuang gold deposit include angular sylvanite and altait, which were disseminated in the space between anhedral quartz and sheet muscovite. The Te-bearing minerals have particle sizes ranging from a few to hundreds of micrometers. Microanalysis of the Te-bearing minerals indicates that the chemical formulae of sylvanite and altaite are Ag0.85Au2.55Te6 and Pb0.52Te0.48, respectively. Sylvanite has an abnormal chemical composition. Through the study, an advanced and practical technique for analyzing the composition and the morphological characteristics of Te minerals and their relationship with wallrock was developed. This study also provides technique support for comprehensive utilization of Te-bearing gold minerals.

Building a comprehensive project-based curriculum based on the PBL teaching philosophy to meet the requirements of cultivating practical and comprehensive innovative talents in high-level, challenging, and innovative first-class courses in universities. Guided by STEAM and taking the course of "practice and innovation training based on ukulele" as an example, this paper designs the teaching objectives and content of PBL teaching, analyzes the corresponding teaching organization and teacher team construction according to the teaching characteristics, and conducts teaching research and practice. [ABSTRACT FROM AUTHOR]

[Objectives] To study the effects of different slow-release carriers on the release rate of plant volatiles, in order to provide technical support for the selection of appropriate release carriers for the research of related insect attractants, so as to achieve the best trapping effect and longer duration. [Methods] The release rate and duration of α-pinene and cis-3-hexenol in LDPE bottles and LDPE bags with different specifications were measured by weighing method under outdoor conditions. [Results] The release rate test of LDPE bottles showed that there were significant differences in the release rates of plant volatiles between different sizes of LDPE bottles and different doses. The average daily release rate of 15 mL LDPE bottle +15 mL α-pinene within 16 days was the lowest, which was (268.3±70.3) mg⋅d-1, significantly lower than (580.0±119.5) mg⋅d-1 of 100 mL LDPE bottle +15 mL α-pinene and (913.3±133.8) mg⋅d-1 of 100 mL LDPE bottle +50 mL α-pinene. The duration of 100 mL LDPE bottle +15 mL α-pinene combination is about 16 days, while the duration of 15 mL LDPE bottle +15 mL α-pinene combination and 100 mL LDPE bottle+50 mL α-pinene combination can reach more than 50 days. The release rate test of LDPE bag showed that, The average daily release rate of 2 mL cis-3-hexenol in 0.02 mm LDPE bag within 12 days was the highest, which was (110.7±28.0) mg⋅d-1, significantly higher than that of other thickness LDPE bags; The daily average release rate of 2 mL cis-3-hexenol in 0.06, 0.08, 0.10 and 0.16 mm LDPE bags were (48.3±14.4), (42.0±11.5), (36.3±14.4) and (31.0±7.9) mg⋅d-1, with no significant difference; The duration of 2 mL cis-3-hexenol in 0.02 mm LDPE bag is about 16 days, while the release rate on 0.06-0.16 mm LDPE bags is relatively stable, and the duration can reach 50-60 days. [Conclusion] The test results show that the release rate can be accurately controlled by using different volumes, different thicknesses of slow-release carriers and different doses of plant volatiles to meet the needs of different insect attractants. The conclusion of this study can provide some reference for the selection of slow-release carriers of insect attractants in production, and provide technical support for insect pest monitoring and pest control. [ABSTRACT FROM AUTHOR]

To reduce the cost of TD-SCDMA network construction,a solution of base station location planning problem based on immune computing was proposed.The difficulties of TD-SCDMA base station construction and the principle of location planning were expound,clonal multiplication operator,clonal mutation and clonal selection operator with real-number encoding were designed,the framework of immune memory clonal algorithm for base station location plan-ning problem was given,and simulation experiments were done to validate the proposed algorithm.Experimental result shows that the proposed solution can obtain higher network coverage rate with lower cost of network construction rela-tively,and has the advantage of good application value.

The spectrum sensing process was described based on WRAN.By analyzing physical connection of cognitive wireless network,the mathematical model of spectrum allocation was given,and then it was converted into a constrained optimization problem,whose goal was to maximum the profit of network.An immune clone selection algorithm was proposed to solve the problem,and the algorithm convergent with probability 1 was proved.Finally,the simulation experiments were done to test the algorithm.The results showed that compared with CSGC algorithm（color-sensitive graph coloring） and GA-SA（spectrum allocation based on genetic algorithm）,this algorithm could achieve maximization of network profits.Meanwhile,the system simulation results based on WRAN confirmed its effectiveness.

New cognitive radio channel model is the combination of the interweave channel and the interference channel.By introducing the switch concept,the spectrum hole sensing was combined the channel model.So a new cognitive radio two-user channel model with partial knowledge of the primary messages at the cognitive transmitter was proposed.First,the channel between the primary user and the second user was modeled as a compression channel and introduced the compression rate.Then,the channel capacity was analyzed and calculated.Finally,numerical results illustrate the validity of the new channel model and the advantage of its capacity.