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Objective: This study aimed to investigate the effect of Antarctic krill oil on zebrafish model of depression. Methods: The zebrafish (Danio rerio) model of depression was induced by 40 mg/L reserpine, and five groups, i.e., blank control group, model group, fluoxetine group, low-dose krill oil group and high-dose krill oil group were investigated and compared, buy using new tank test (NTT) and light dark box test (LDB). The central nervous system serotonin (5-HT), dopamine (DA), monoamine oxidase A (MAO-A) Interleukin-6 (IL-6), tumor necrosis factor α (TNF-α) and systemic cortisol (COR) levels of the five groups were analyzed. Results: Reserpine 40 mg/L caused depressive behavior in zebrafish, while the addition of prawn oil reduced the this behavior, by increasing the levels of monoamine neurotransmitters including 5-HT and DA in the brain and decreasing the levels of MAO-A, IL-6 and TNF-α proinflammatory factors and systemic COR levels. Conclusion: Antarctic krill oil can improve the depression of zebrafish by reducing neuroinflammation and regulating neuroendocrine.

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Super enhancers (SEs) are composed of clusters of enhancers in close genomic proximity. They constitute a large family of regulatory elements that specify gene expression patterns and cell identity. SE regions consist of unusually strong enrichment of binding sites for transcriptional factors, cofactors, and enhancers associated with epigenetic modifications. SEs play important roles in regulating the aberrant gene expression in tumor cells. Via SEs, cancer cells activate the expression of various oncogenes, and promote cell proliferation, invasion and migration properties. Hence suppression of SEs activities could inhibit the growth and survival of cancer cells. In this review, we summarize the fundamental principles, functions and regulation of super enhancers and therapeutic potential in targeting SEs in cancer cells, thereby introducing and providing new conceptions for development of antineoplastic drugs.

Effects of river (Yangtze River)-lake (Poyang Lake) relation on the distribution and potential ecological risk of Cu, Pb, Zn, Cr and Cd were investigated in surface sediment of Poyang Lake under different waterregimens. The results indicated: (1) the sediments of Poyang Lake were polluted by various concentrations of heavy metals mainly originated from particulates of Yangtze River, and Cu and Pb were the main pollution factors. The pollution level of determined heavy metals followed the order of Cu > Pb > Zn > Cr >Cd. The concentration ranges of Cu, Pb, Zn, Cr and Cd in the sediment of Poyang Lake were 13.1-108.1 mg.kg-1 , 37-119.1 mg.kg-1, 29.9-129.9 mgkg-1, 13.3-98.6 mgkg-1 and 0. 19-2.77 mg.kg-1 during the wet period, and 3.05-69.7 mg.kg-1, 27.5-105 mgkg-1, 18.8-95.4 mg.kg-1, 7.34-70 mg.kg- and 0.033-0.406 mg.kg-1 during the dry period respectively. The region with the highest heavy metal concentrations was located in water input area of the " Five River" and water output area of " Hukou" ; (2)The regions with high risk of heavy metals in sediment were mainly located in the area of the trail of "Five Rivers" during the wet period; while during the dry period, the area with high risk of heavy metals in sediment enlarged, which was not only limited in the area of the trail of "Five Rivers", but also enlarged northwards. The potential ecological risk of "Hukou" was relatively high, however, the potential ecological risk of the sediment in the whole lake during the dry period was lower than that during the wet period. (3) With the change of river-lake relation, water level rose, the maintaining time was shortened during the wet period, the dry season appeared ahead of schedule, the transformation course of Poyang Lake from "Lake morphology" to " River morphology" increased, and the typical river properties were enhanced, which resulted in the decreased potential ecological risk of heavy metals in sediment of the whole lake, however, the area of high risk-region was enlarged northwards.

Adenovirus remains a significant threat to public health. Recent studies showed that bats can harbor diverse adenoviruses. To further investigate the distribution and genetic diversity of bat adenoviruses in China, we collected throat and anal swab samples of 11 bat species from 6 provinces of China, including Beijing, Hunan, Jiangxi, Yunnan, Guizhou and Hainan. Nested PCR was used to identify potential bat adenoviruses from the samples, and positive results were cloned and sequenced for genetic diversity study. In addition, nucleotide sequence alignments based on corresponding amino acid sequence similarities were used for phylogenetic analyses. Our results showed that about 20% of bat species in China are positive to adenoviruses, and Myotis ricketti is likely to be the most important host of bat adenoviruses in all locations. Moreover, we identified two diverse sequences of bat adenoviruses from the same sample of Ia io in Guizhou province of China. In general, the average nucleotide and amino acid sequence similarities of the conserved region of DNA polymerases of bat adenoviruses are 66.6% and 74.7%, respectively. The differences between bat species and their residences environments may have driven the adaptive evolution of the viruses, leading to the genetic diversity of the bat adenoviruses.

An outbreak of hand foot and mouth disease occurred in Shang dong, China in 2009. Almost 20% of patient's swabs was positive for Coxsackie virus B5 (CVB5) identified by RT-PCR and sequencing. It was suggested that CVB5 may be another important pathogen for HFMD. Fifteen pairs of overlapping primers were designed and the genome sequence was sequenced. The genome of CVB5 was 7 399 nt in length, coding for 2 185aa. The genome displayed 80.6%-85.3% nucleotide sequence identity and 96.1%-96.9% amino acid sequence identity with another three CVB5 respectively. Phylogenetic analysis showed that different segment of genome underwent a distinct evolutionary and selective pressure. Simplot analysis displayed no evident recombination between genome of CVB5 and other HEV B viruses. The complete and characterized genome of CVB5/09 provides further insight into the genetics of CVB5 and other HEV B viruses, aiding in the surveillance and control of HFMD.

Objective: To discuss the clinical value of percutaneous transhepatic cholangial drainage (PTCD) combined with nasojejunal tube for bile reinfusion and enteral nutrition for patients with malignant obstructive jaundice., Methods: Forty patients with malignant obstructive jaundice were randomly divided into bile reinfusion group (n=20) and exclusive external drainage group (control group, n=20), and the clinical data concerning the hepatic function, visceral protein and postoperative complications of the patients were collected., Results: In both of the two groups, the levels of ALT, AST, and TB-2 reduced significantly after the operation as compared with the preperative levels (P<0.05), and no significant difference was found in the postoperative hepatic function between the two groups (P>0.05). The postoperative levels of the visceral proteins such as ALB, TRF and PRE increased significantly after the operation (P<0.05), and the changes in ALB and PRE were comparable between the two groups (P>0.05). TRF was significantly higher in bile reinfusion group than in the control group., Conclusion: PTCD combined with bile reinfusion and early enteral nutrition via the nasojejunal tube may facilitate the recovery of hepatic function and visceral proteins in patients with malignant obstructive jaundice.

Objective: To investigate the expression of haptoglobin in the lesions of condyloma acuminatum (CA) at the mRNA and protein level, and to explore its role in the pathogenesis of CA., Methods: The expressions of haptoglobin protein and mRNA in the skin tissues of 30 patients with CA and 20 normal controls were detected by immunohistochemistry(IHC), Western blot, and hybridization in situ., Results: The in situ hybridization study showed that haptoglobin mRNA was expressed in the epidermal cells in the lesions of CA. The distribution of haptoglobin mRNA expression in the lesions of CA was similar to that of the normal controls, and the expression of haptoglobin mRNA in CA was higher than that of the normal controls. There was a significant difference in the positive expression of haptoglobin mRNA between the CA group and the control group (P<0.05). The immunohistochemical study showed that haptoglobin protein was expressed in the whole layers of epidermal keratinocytes in the lesions of CA at a high level and stronger staining was seen in the stratum basale and stratum spinosum. Haptoglobin protein was expressed predominantly in the stratum basale in normal skin tissues, while weak staining was seen below the stratum spinosum.There was a significant difference in the mean gray value between the CA group and control group (P<0.05). Western blot showed that the haptoglobin expression in CA lesions significantly increased compared with the normal skins (P<0.05)., Conclusion: The expression of haptoglobin mRNA in the CA lesions obviously increases and the epidermal cells in the CA lesions are able to synthesize haptoglobin protein. Haptoglobin in the CA lesions may involve in the local immunity escape by preventing Langerhans cell functional maturation and inhibiting the immunocompetence of keratinocyte.

Somatic mutation in the tyrosine kinase (TK) domain of the epidermal growth factor receptor (EGFR) gene is associated with the sensitivity of non-smal cell lung cancer (NSCLC) to TK inhibitor Gefitinib. Mutational analysis for EGFR exons 19 and 21 was performed in 75 NSCLC and 10 breast cancer patients. All patients had not received treatment of Gefitinib. Somatic mutations in TK domain of EGFR were identified in 13 of the 75(13/75, 17.33%) patients, including 7 cases of in-frame deletion in exon 19 (7/75, 9.33%) and 6 cases of amino acid substitution (2573T>G, L858R) in exon 21 (6/75, 8%) . No other mutations were found in 10 breast cancer patients who stained positive for HER2 immunhistochemistry. Adenocarcinoma has a higher rate of mutations than several other types of NSCLC, the mutations occurring more fre-quently in female patients. EGFR mutation rate in Chinese NSCLC patients was higher than that in Caucasians. Our data indicated that Chinese adenocarcinoma patients could benefit from TK inhibitor Gefitinib.

According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.

Purpose: To evaluate the effect of using tissue engineered oral mucosa lamina proprias to repair buccal mucosal full-thickness defects in rats., Methods: Cultured oral mucosal fibroblasts(OMF) of Wistar rat were incorporated into chitosan-collagen to construct tissue engineered oral mucosa lamina proprias, then the OMFs were labeled with 5-BrdU. Full-thickness round mucosal defects were made with round knife (diameter of 0.8 cm) on the buccal mucosas of 21 Wistar rats(21-25 weeks old) which were divided in fibroblast-populated chitosan collagen lattices(FPCCL) group with FPCCLs transplantation and control group covered with gauges alone. All wounds were observed by macrograph or under optical microscopy and measured on 4th, 7th, 14th and/or 21st day postoperatively. The data were analyzed by SPSS11.5 software package for Student's t test., Results: There were no infection through the wound healing process. On the 7th day after grafting, the wounds of two groups were covered by yellow membrane, on the 14th, 21st day, the wounds of two groups were covered by new epidermis thoroughly, the color of new epidermis was similar to the normal one. On the 7th day, the wound diameter of control group was significantly smaller than that of FPCCL group (P<0.05). Under optical microscopy each mucosal wound in two groups was only partly keratinocyted with new granular tissue rich in inflammatory cells, fibroblasts, new vessels on the 7th day postoperatively. The wounds in two groups were keratinocyted thoroughly with rete ridges and horny layer on 14th and 21st day. OMFs with 5-BrdU appeared in the granular tissue and new lamina propria on 7th, 14th and 21st day postoperatively by immunohistochemical staining, OMFs together with granular tissue were joined in the repair of buccal mucosal defects without allergic reaction through the whole wound healing process., Conclusions: It is suggested that OMFs as the seed cells can join in the repair of buccal mucosa defects effectively; chitosan-collagen lattices have good histocompatibility; Limitation of contraction of recipient wounds will be closely related to FPCCLs; therefore, FPCCLs can repair buccal mucosal defects effectively.

Objective: To investigate the development method of hydrophobic coating porous plates for water sublimator and carry out an experimental study on hydrophobic coating porous plates., Method: Porous plates were coated with Teflon coating solution and a kind of special hydrophobic coating solution. Physical characteristic tests for hydrophobic coating porous plates were conducted. An upgrading breadboard of sublimator was redesigned. Effects of hydrophobic coating porous plates on heat rejection of water sublimator were studied., Result: Hydrophobic coating porous plate could evidently increase the water retention pressure, but also increase pressure drop of gas permeability in varying degrees. Heat rejection performance of two kinds of coating plates was different. Performance of coating plate with the special hydrophobic coating solution was better., Conclusion: Hydrophobic coating porous plate could improve performance of water sublimator by optimizing coating parameters and technics of Teflon coating solution and the special hydrophobic coating solution.