The experiment was to evaluate the nutritional value of hullessbarley straw by white-rot fungi fermentation using Cornell Net Carbohydrate-Protein System (CNCPS). Three white rot fungi (Pleurotus sajor-caju, Pleurotus ostreatus, Pleurotus rhodophyllus Bres) were selected to ferment highland barley straw, and CK group was not inoculated with strains on highland barley straw. After 21 d of solid-state fermentation, various nutritional indicators in fermentation substrate were measured to evaluate the fermentation effect. The results showed that the content of CP, EE, SP and NPN of P. sajor-caju group were extremely higher than other groups (P<0.01), the content of NDF, ADF, ADL, NDFIP and ADFIP were extremely lower than other groups (P<0.01). The content of PA, PB2 and PB3 of P. sajor-caju group and P. ostreatus group were extremely higher than CK group and P. rhodophyllus Bres group (P<0.01), the content of PB1 of P. sajor-caju group and P. rhodophyllus Bres group was extremely higher than CK group and P. ostreatus group (P<0.01), and the content of PC of P. sajor-caju group was extremely lower than other groups (P<0.01). The content of CHO of P. rhodophyllus Bres group was extremely higher than other groups (P<0.01), the content of CA, CB1, CB2 and CNSC of P. sajor-caju were extremely higher than other groups (P<0.01), and content of CC was extremely lower than other groups (P<0.01). The experiment indicates that the white rot fungi fermentation can effectively improve the nutrition value of hullessbarley straw, and the best fermented impact is achieved in the Pleurotus sajor-caju treatment. [ABSTRACT FROM AUTHOR]