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A new high density alloy NiW750 was developed, which was based on the faced-center cubic solid solution structure and aging strengthening mechanism. Microstructure of the alloy was observed by SEM and TEM. The material characteristics under dynamic compressing loading were investigated by using the split Hopkinson pressure bar test. The comparison among the NiW750, ultra high strength steel G50 and tungsten 93WNiFe was also conducted.The results show that the NiW750 high density alloy has the best comprehensive properties among three materials. After aging at 750℃/5h,the tensile strength of NiW750 can achieve up to 1746MPa,while the impact toughness (akU) can achieve 113J/cm2. Under the condition of dynamic loading, the material shows strain rate hardening effect obviously as its dynamic flow stress can reach about 2250MPa. The adiabatic shear bands are formed within specimens in the direction of 45° to the central axis with a bandwidth of 80-150μm at the strain rate of about 5500s-1 and a wide transition zone, so as to avoid the premature emergence of the shear fracture.

Objective This study aims to analyze the clinical, pathological and dermoscopic of lichen planus (LP) patients from Jiangsu Provincial Hospital of Traditional Chinese Medicine. Methods A comprehensive analysis of clinical pathology data of 82 LP patients and dermoscopic images of 103 LP patients from January 2011 to February 2024 was conducted. Results Among the 185 LP patients, 70 were male and 115 were female, the ratio of male to female was 1-1.64. Ages ranged from 7 to 82 years, with a mean age was 45.34 ± 16.46 years. The clinical manifestations were diverse, presenting as generalized or localized eruptions, the primary affected areas on the limbs in 93 cases (50. 21%). The lesions primarily manifested as papules or plaques in 142 cases (76.76%). Histopathological examination mainly showed liquefaction degeneration of the basal layer and band-like infiltration of lymphocytes in the superficial dermis, present in 82 cases (100.00%) and 76 cases (92.68%), respectively. The concordance rate between clinical and pathological diagnoses was 62. 20%. Reflectance confocal microscopy images showed liquefaction degeneration of the basal layer in 65 cases (100. 00%) and pigment-laden macrophages and inflammatory cell infiltration in the dermal papillae in 60 cases (92. 31%). Dermoscopy examination observed Wickham striae in 32 cases (84.21%). Conclusion Site involvement, coloration of lesions, Wickham's striae, and lesion arrangement are pivotal diagnostic indicators for LP. Dermoscopy aids in detecting Wickham's striae, while reflectance confocal microscopy emerges as a crucial screening modality. Histopathological examination remains integral for definitive diagnosis. [ABSTRACT FROM AUTHOR]

The concentration, complexity, and significant anisotropy of in-situ stress make it a pressing and challenging issue in engineering geology safety in strong tectonic activity areas. This paper firstly analyzes the application and existing problems of in-situ stress measurement in deep-buried underground engineering in strong tectonic activity areas. Then, it focuses on the application method, technology, and roles of real-time in-situ stress monitoring in deep underground engineering within tectonically active regions. Finally, it discusses the problems that need to be considered in the application of in-situ stress measurement and real-time monitoring. The results show that in the strong tectonic activity area, relying solely on limited deep hole in-situ stress measurements to determine overall stress design parameters for deep underground engineering is inadequate. A comprehensive study of the three-dimensional in-situ stress field is necessary to reveal its spatial distribution characteristics. Different in-situ stress design parameters should be used for different positions of the deep-buried underground project to avoid engineering waste or engineering damages caused by large or small in-situ stress design parameters. In the strong tectonic activity area, the disk core density is inversely proportional to the measured magnitude of the in-situ stress, and the depth range that has yet to form in the cake-shaped core often has the highest in-situ stress and the most concentrated stress, and the deep underground engineering should avoid this depth range. While a major earthquake or major engineering geological problem occurs, real-time monitoring of in-situ stress can dynamically reveal the relative change trend and evolution process of the in-situ stress magnitude of a specific structural site. It can calculate the absolute value of the in-situ stress state in different time domains during the real-time monitoring period without carrying out new absolute in-situ stress measurements. Regional crust stability and deep-buried engineering geological safety risks can be quickly evaluated, and quantitative in-situ stress design parameters and the stress-strain reserved threshold for preventing deformation and breaking can be provided for the damage repair of deep-buried engineering and the risk of fault activity can also be assessed. The research results will offer geological security for the planning, construction, and safe operation and maintenance of major projects in strong tectonic activity areas.

The interlaced underwater distributary channel sand bodies are developed in Chang 4+5 reservoir group in Jiyuan area， and their internal contact relationships are complex， which seriously affects the reservoir distribution law and water flooding development effect in this area. It is beneficial to the sustained and stable production and efficient development of Chang 4+5 reservoir in Jiyuan area by the fine study of identification and division of single sand body interface， analyzing the contact modes of sand bodes and their influence on water flooding effect in Chang 4+5 reservoir. Based on the identification and division of the single sand body interface， the core， logging， and dynamic production data are comprehensively applied to delineate the internal contact relationships of sand bodies， and then clarify the influence of transverse cross and vertical superposition relationships of sand bodies on water flooding effect. The results show that there are three vertical superposition relationships of sand bodies in Chang 4+5 reservoir in Jiyuan area， namely cut-stacked， superimposed， and separated. Among them， the cut-stacked sand bodies are more homogeneous， with high oil content and percentage of producing reserves under water flooding， followed by the superimposed sand body. The lateral contact modes of single sand bodies can be divided into three types： isolated mode， docking mode， and side-cut mode. Different contact modes reflect the difference in interwell connectivity of channel sand bodies， which in turn affects the effective time of water injection in oil wells. The effective time of water injection in oil wells corresponding to the side cut mode is short， followed by the docking mode， and the effect of water injection in oil wells corresponding to the isolated sand body is not obvious.

The Shunyi fault in the Beijing Plain region is a significant late Pleistocene active fault. The Beijing Capital International Airport (BCIA) is situated within the central segment of the Shunyi Fault. Since 2010, ground fissures on the airport's runway have progressively worsened, with a maximum vertical displacement difference of up to 20 cm, severely affecting airport safety. Presently, the impact of Shunyi fault activity on the ground fissures at the BCIA is primarily described qualitatively. This paper, based on research regarding the geometric structure of the Shunyi Fault, Quaternary activity, and investigations of the ground fissures at the Capital Airport, employs the fault dislocation model to quantitatively analyze the influence of the Shunyi Fault's creep-sliding activity as well as the 1996 Shunyi ML 4.5 earthquake on the formation of airport ground fissures. The study also discusses the increasing risk of ground fissure hazards in the event of a potential strong earthquake along the Shunyi fault in the future. The preliminary results suggest that with a vertical activity rate of 0.6 mm/year, 46 years of Shunyi Fault's creep-sliding activity results in differential subsidence of no more than 2.5 cm on both sides of the airport ground fissures, contributing to approximately 20% of the formation and development of these fissures. The impact of the Shunyi ML 4.5 earthquake on the formation of airport ground fissures is minimal. However, if a future M 7.0 earthquake occurs on the Shunyi fault, the estimated maximum land differential subsidence between two sides of the fault could reach 104 cm, increasing the risk of airport ground fissure disasters by a factor of five. Ground differential settlement due to groundwater extraction from the upper and deeper layers of the Shunyi fault's hanging wall contributes about 70% to the formation and development of airport ground fissures, remaining the primary factor. The study's results provide essential scientific references for precise prevention and control of ground fissure disasters at BCIA. Furthermore, to further reveal the causes and mechanisms of delayed geological disasters along the Shunyi fault, such as ground fissures and land differential subsidence, it is recommended to implement dynamic monitoring of cross-fault crustal displacement or deformation at crucial segments.

The Namjag Barwa syntaxis is in the eastern Himalayan syntaxis area with the most intensive neotectonic activity. There are many late Quaternary active fault belts and strong seismicities. The tectonic stability of these active fault belts, such as the Jiali, Dongjiu-Milin, and Motuo fault belts, may influence the project's construction. In-situ stress is a critical parameter for estimating regional tectonic stability. Currently, there is a lack of abundant in-situ stress results about the Namjag Barwa syntaxis. It is challenging to assess geological safety risks for major projects. Based on focal mechanism solutions, the paper reveals the orientation of the maximum principal stress surrounding the Namjag Barwa syntaxis using the stress tensor inversion method. According to the critical condition of fault instability, the magnitudes of principal stresses around the Namjag Barwa syntaxis are also estimated by combining the inversion of the stress shape ratio and the frictional coefficient. The results indicate that the maximum principal stress direction in the Namjag Barwa syntaxis area is NE-NNE. The maximum and minimum horizontal principal stresses increase linearly with depth at a gradient of 0.032~0.0355 MPa/m and 0.0227~0.0236 MPa/m, respectively. Heterogeneous features of the in-situ stress field still exist. Generally, the results estimated in this study are in good concordance with the in-situ stress measurements. They can provide reliable in-situ stress parameters for evaluating the tectonic stability in the Namcha Barwa region.

The eastern Himalayan syntaxis is one of the regions having the most intense tectonic activities, the most complex geological conditions, and the most frequent geohazards in the world. The planning and construction of engineering projects are faced with four types of catastrophic geo-safety risks, including tectonic faulting in the plate tectonic belt, disaster occurrence of the deep-buried tunnels, instability of loose mountains, and regional geological disaster chain. It is a critical topic in the field of engineering geology how to select relatively stable and safe sites in active tectonic zones to minimize the geo-safety risks of planning, construction, and operation of engineering projects. This paper summarized the major geo-safety problems in the eastern Himalayan syntaxis. Accordingly, it revealed that traditional site selection theories hardly satisfy the requirements of the engineering projects in the eastern Himalayan syntaxis area. The site selection encounters geo-safety challenges caused by unclear geological evolution process and construction, prominent disaster risk of tectonic activity and strong earthquake, weak research on the deep tectonic stress field and disaster evaluation, and severe ultra-high-elevation and ultra-long-runout geological disaster chain. Thus, this paper suggested the main research directions of the site selection from five aspects: (1) regional geological evolution and engineering geological problems, (2) active fault and engineering safety risk, (3) complex in-situ stress field and engineering disaster risk, (4) engineering risk of regional geological disaster chain, and (5) theory and method of site selection in eastern Himalayan syntaxis. This paper provides ideas for improving the risk assessment and prevention methods of site selection of engineering projects.

Objective To study the association of the polymorphisms of the serum amyloid A1 (SAA1) gene at rs4638289 and rs7131332 loci with Kawasaki disease (KD) and its complication coronary artery lesion (CAL) in children. Methods A total of 105 Han children with KD who were hospitalized and treated from 2013 to 2017 were enrolled as the KD group. A total of 100 Han children who underwent physical examination were enrolled as the control group. According to the presence or absence of CAL, the KD group was further divided into a CAL group with 23 children and a non-CAL (NCAL) group with 82 children. Polymerase chain reaction-restriction fragment length polymorphism was used to investigate the polymorphisms of the SAA1 gene at rs4638289 and rs7131332 loci. Results For the locus rs4638289 of the SAA1 gene, there were no significant differences between the KD and control groups in the genotype frequencies of AA, AT, and TT and the allele frequencies of A and T (P>0.05). But there were significant differences between the CAL and NCAL groups in the genotype frequencies of AA, AT, and TT (P=0.016), while there were no significant differences in the allele frequencies of A and T (P>0.05). AT genotype was a protective factor against CAL (OR=0.276, 95%CI: 0.099-0.772, P=0.011). For the locus rs7131332 of the SAA1 gene, there were no significant differences between the KD and control groups in the genotype frequencies of AA, AG, and GG and the allele frequencies of A and G (P>0.05). There were also no significant differences between the CAL and NCAL groups in the genotype frequencies of AA, AG, and GG and the allele frequencies of A and G (P>0.05). Conclusions Polymorphisms of the SAA1 gene at loci rs4638289 and rs7131332 are not associated with the onset of KD, while the polymorphism at the locus rs4638289 is associated with CAL in KD patients. KD patients with genotype AT may have a reduced risk of CAL. [ABSTRACT FROM AUTHOR]

Objective To screen out the differential expression circRNA in peripheral blood of patients with aortic regurgitation and left ventricular enlargement. Methods Four patients with aortic regurgitation complicated with left ventricular enlargement (observation group) and four patients with aortic valve regurgitation without left ventricular enlargement (control group) were selected. Their fasting venous blood was taken to detect the circRNAs by high throughput circRNA chip technique, and differentially expressed circRNAs between the two groups were screened out. Target Scan ant miRanda software were used to predict the circRNAs which were associated with miRNA-233 related to myocardial remodeling. Results Compared with the control group of fluorescence signal intensity, 158 circRNAs were differentially expressed, with fold change values> 2 ( P < 0. 05) including 21 circRNAs' fold change values > 5, meanwhile, 105 were down-regulated and 53 were up-regulated. There were binding sites between hsa-circRNA-102898, hsa-circRNA-101661, hsa-circRNA-103740 and miRNA-233 of 158 differentially expressed circRNAs. Conclusions Compared with patients with aortic regurgitation without left ventricular enlargement, there are 158 differentially expressed circRNAs in peripheral blood of patients with aortic regurgitation and left ventricular enlargement. Twenty-one circRNAs, such as hsa-circRNA-103245, hsa-circRNA-104014, hs-circRNA-102617, and hsa-circRNA-103421 are significantly different. Totally 158 differential expressed circRNAs may be associated with the occurrence and development of left ventricular enlargement with aortic regurgitation. The hsa-circRNA-102898, hsa-circRNA-101661, and hsa-circRNA-103740 may regulate left ventricular enlargement by binding to miRNA-233 site. [ABSTRACT FROM AUTHOR]

Based on the mode of C/S, using VB language and data management, we developed a management system of antenna testing on the VB. Net technical platform. The system changes the way of mainly manual testing in most factories, into automatic testing, and uploads the measured data to the server. Simultaneously, manager can regularize all departments assignments according to the manufacture requirement, know the testing result and performance of the production line The system strengthens the management and supervise of industrial production. The method prementioned has improved the testing efficiency, solved the product traceability problem. [ABSTRACT FROM AUTHOR]

In order to solve the traditional A* algorithm is not taken into account when planning path obstacle distribution on the influence of the path selection,this paper puts forward an improved A* algorithm,the artificial potential field of thought and the traditional A* algorithm,the combination of the obstacles in grid map gives repulsive force function and the repulsive force around the grid size calculation,in order to improve the searching ability of A* algorithm,the repulsive force of the grid is introduced into the evaluation function of A* algorithm.The results of MATLAB simulation and Turtlebot experiments show that,compared with the traditional A* algorithm,the new improved algorithm combined with artificial potential field algorithm can plan a better path,improve the efficiency of path planning,and increase the search speed by 13.40%~29.68%.The path length is shortened by 10.56%~24.38%,and the number of path nodes is reduced by 6.89%~27.27%.The experimental results show that the improved A* algorithm has obvious optimization effect and is effective and feasible.

Objective To construct human TNC expression plasmid for the investigation of the role of TNC in pancreatic cancer metastasis by using pancreatic cancer cell strain with stable over-expression of TNC. Methods TNC expression plasmid was constructed by unique primer design method, and then pancreatic cancer cell strain PANC1 with stable TNC expression were obtained by transfection and G418 screening. RT-qPCR and Western blot examined the expression of TNC. Transwell and Western blot were used to detecte the effects of TNC on cell migration, invasion and EMT-related molecules. Results TNC plasmid was successfully constructed. TNC promoted cell migration and invasion(P＜0.05), decreased epithelial marker E-cadherin expression and upregulated mesenchymal marker N-cadherin expression (P＜0.05). Conclusions The TNC expression plasmid and TNC stable expression pancreatic cancer cell strain may be used to explore the biological function of TNC and the pathogenesis of pancreatic cancer.

To explore the methods of cultivating the clinical thinking ability of acupuncture and moxibustion in the standardized training of resident physicians, so as to improve the medical record writing ability of the regular training physicians. The clinical diagnosis and treatment of acupuncture and moxibustion has its own characteristics and can't copy the syndrome differentiation and treatment mode of TCM internal medicine. In the treatment section, Acupuncture and Moxibustion , a standardized training textbook for national TCM resident physicians, takes clinical cases as the breakthrough point and uses the problem as the guide, guides the training physicians to cultivate acupuncture and moxibustion clinical diagnosis and treatment from three aspects: disease diagnosis, syndrome diagnosis, and treatment ideas, forms a complete understanding of the disease, and improves the standardization, logicality and systematicness of medical record writing through repeated practical training.

Based on the clinical needs and examination requirements of standardized training students, the China national standardized training textbook Acupuncture and Moxibustion for residents of traditional Chinese medicine has made innovations in the textbook content and form. In the part of meridians and acupoints, the classic original text is introduced and the main indications and operation methods of 200 commonly-used acupoints are summarized in the form of tables. In the part of acupuncture and moxibustion technique, the operating procedures are standardized and the core technical points of 20 commonly-used acupuncture and moxibustion techniques are summarized in the form of flow chart. In the part of acupuncture and moxibustion treatment, 48 typical diseases are introduced in the form of case discussion, highlighting the problem orientation and demonstrating the diagnosis and treatment procedures.

Objective: To explore the effect of electroacupuncture (EA) combined with Schwann cell (SC) transplantation (SCT) on remyelination of axons and neuregulin (Nrg1) in rats with compressed spinal cord injury(CSCI),so as to explore the mechanism of EA and SCT underlying improvement of CSCI., Methods: SD female rats were randomly divided into normal, mo-del, EA, SCT, and EA+ SCT groups ( n =40 per group). A self-developed model of spinal compressed injury was adopted in this study. Rats of the model group were administrated laminectomy without treatment. Rats in the EA group were administrated EA stimulation at "Dazhui"(GV14), "Mingmen"(GV7), bilateral "Zusanli" (ST36) and "Taixi" (KI3) on the second day post-surgery for 10 min. Rats in the SC group were administrated SCT at 1 week post-surgery, and in the EA+SC group were given EA stimulation combined with SCT. The injured spinal cord tissue was obtained 0, 2, 4 and 8 weeks after compressed spinal injury. The functional recovery was assessed by Basso-Beattie-Bresnahan (BBB) score. The survivals and migration of SC after transplantation, myelination were observed by immunofluorescence. The ultrastructure of myelin in injured site was observed by transmission electron microscope，and the expression levels of glial fibrillary acidic protein (GFAP), protein zero(P0), and Nrg1 and Nrg1-ntf (cleavage protein of Nrg1) proteins of the spinal cord were detected by Western blot., Results: Compared with the normal group, BBB scores in the model group was significantly decreased（ P <0.05）,nervous fibers were demyelinated, numbers of normal and newborn myelination were decreased( P <0.05)，expression of P0 was significantly increased ( P <0.05)，expression of GFAP was significantly increased( P <0.05)，and the expression levels of Nrg1 and Nrg1-ntf proteins were decreased( P <0.05). In comparison with the model group, the BBB scores in the EA, SCT and EA+SCT groups were significantly increased（ P <0.05, P <0.01）, demyelination was improved, numbers of normal and newborn myelinations were increased( P <0.05, P <0.01)，expressions of P0 were significantly increased ( P <0.05, P <0.01)，expressions of GFAP were significantly decreased( P <0.05)，and the expression levels of Nrg1 and Nrg1-ntf proteins were increased ( P <0.05, P <0.01).The differences were most significant in the EA+SCT group among the three groups., Conclusion: EA can improve the locomotor function in CSCI rats, which may be rela-ted to its functions in promoting the survival and migration of transplanted SC and remyelination, and increasing the expressions of Nrg1 and its cleavage protein after SC transplantation.

Objective: To study the association of the polymorphisms of the serum amyloid A1 (SAA1) gene at rs4638289 and rs7131332 loci with Kawasaki disease (KD) and its complication coronary artery lesion (CAL) in children., Methods: A total of 105 Han children with KD who were hospitalized and treated from 2013 to 2017 were enrolled as the KD group. A total of 100 Han children who underwent physical examination were enrolled as the control group. According to the presence or absence of CAL, the KD group was further divided into a CAL group with 23 children and a non-CAL (NCAL) group with 82 children. Polymerase chain reaction-restriction fragment length polymorphism was used to investigate the polymorphisms of the SAA1 gene at rs4638289 and rs7131332 loci., Resukts: For the locus rs4638289 of the SAA1 gene, there were no significant differences between the KD and control groups in the genotype frequencies of AA, AT, and TT and the allele frequencies of A and T (P>0.05). But there were significant differences between the CAL and NCAL groups in the genotype frequencies of AA, AT, and TT (P=0.016), while there were no significant differences in the allele frequencies of A and T (P>0.05). AT genotype was a protective factor against CAL (OR=0.276, 95%CI: 0.099-0.772, P=0.011). For the locus rs7131332 of the SAA1 gene, there were no significant differences between the KD and control groups in the genotype frequencies of AA, AG, and GG and the allele frequencies of A and G (P>0.05). There were also no significant differences between the CAL and NCAL groups in the genotype frequencies of AA, AG, and GG and the allele frequencies of A and G (P>0.05)., Conclusions: Polymorphisms of the SAA1 gene at loci rs4638289 and rs7131332 are not associated with the onset of KD, while the polymorphism at the locus rs4638289 is associated with CAL in KD patients. KD patients with genotype AT may have a reduced risk of CAL.

Objective: To study the association of T-wave amplitude on electrocardiogram (ECG) with left ventricular ejection fraction (LVEF) in children with dilated cardiomyopathy., Methods: A retrospective analysis was performed for the clinical data of 44 children who were diagnosed with dilated cardiomyopathy from May 2009 to June 2018. According to LVEF, they were divided into two groups: LVEF ≥50% group (n=26) and LVEF <50% group (n=18). After treatment, 25 children were followed up for 3-42 months (mean 14±9 months). The Guangdong Zhongshan SR-1000A ECG Automatic Analyzer was used to obtain the 12-lead body surface ECG results in the supine position. T-wave amplitude on ECG was evaluated by software and manual measurement., Results: Compared with the LVEF ≥50% group, the LVEF <50% group had a significant reduction in the T-wave amplitude in leads II, V4, V5 and V6 (P<0.05). The increased-LVEF group (an increase in LVEF > 5% after treatment) had a significant increase in the T-wave amplitude in leads aVR, V5, and V6 after treatment (P<0.05), while the unchanged-LVEF group (an increase in LVEF ≤ 5% after treatment) had a significant reduction in the T-wave amplitude in lead aVR after treatment (P<0.05). The receiver operating characteristic curve analysis showed that the T-wave amplitude in leads II, V4, V5 and V6 had a certain value in predicting LVEF <50% in children with dilated cardiomyopathy (P<0.05). A combination of T-wave amplitude of ≤0.20 mV in lead II, ≤0.40 mV in lead V4, and ≤0.30 mV in leads V5 and V6 had a sensitivity of 88.2% and specificity of 76.0% in the predication of LVEF <50% in children with dilated cardiomyopathy., Conclusions: T-wave amplitude on ECG can be used as the indexes for the evaluation of the left ventricular systolic function in children with dilated cardiomyopathy.

Evidence - based Clinical Practice Guideline of Acupuncture and Moxibustion for Primary Dysmenorrhea ( Guideline ) is clarified from three aspects: the development method, contents and clinical application, which could provide clinical practical strategies suitable for most patients for clinicians. Based on comprehensive literature search and summary of acupuncture practice, the best clinical research evidence is obtained according to the evidence-based method, the evidence-quality evaluation and recommendation grading are introduced. With ancient literature, famous experts' experience and expert consensus, the recommendations of acupuncture-moxibustion for primary dysmenorrhea are developed, which are safe, effective and applicable for clinical treatment. The Guideline could standardize the acupuncture treatment plan for primary dysmenorrhea.

Objective: To observe the effect of electroacupuncture (EA) on the expression of apolipoprotein E (ApoE) and related proteins of inflammation and anti-oxidative stress in spinal cord in mice with spinal cord injury (SCI), so as to explore its mechanisms underlying function repair., Methods: Thirty-six female C57BL/6 mice were equally randomized into 3 groups: sham operation, model and EA. The SCI model was established by clamping the spinal cord for 25 s with a serrefine after laminectomy of the 1 st lumbar vertebra (L1). EA (1.5 Hz/7.5 Hz, 1.0 mA) was applied to bila-teral "Zusanli" (ST36) and "Sanyinjiao" (SP6) for 10 min, once a day for 7 days. The hindlimb locomotor function was assessed according to the state of the range of motion, coordination, claw gesture of the hind leg ankle-joint, trunk stabi-lity and the tail posture by using Basso Mouse Scale(BMS). The histopathological changes of the injured area of the spinal cord were determined by H.E. staining. The expression levels of ApoE, phosphorylated nuclear transcription factor-κB(p-NF-κB), interleukin 1 beta(IL-1β), phosphorylated extracellular regulatory protein kinase(p-ERK1/2), extracellular regulatory protein kinase(ERK1/2), nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxidase-1(HO-1) in the spinal cord were detected by Western blot, and the glial fibrillary acidic protein (GFAP)-positive astrocytes were displayed by immunofluorescence staining., Results: After modeling, the BMS scores were significantly decreased in the model group compared with the sham operation group ( P <0.05). Following EA, the BMS scores were markedly increased in the EA group relevant to the model group ( P <0.05), suggesting an improvement of the hindlimb locomotor function. H.E. stain showed structural disorder with lots of cavities, severe inflammatory infiltration with large quantity of inflammatory cells, and apparent reduction of normal neurons in the injured spinal cord tissue of model group, which was milder in the EA group. The expression levels of ApoE, p-NF-κB, IL-1β, p-ERK1/2 (not ERK1/2), Nrf2 and HO-1 were significantly increased in the model group than those in the sham operation group ( P <0.05). Compared with the model group, the expression levels of ApoE, p-ERK1/2, Nrf2 and HO-1 were further notably up-regulated ( P <0.05), and those of p-NF-κB and IL-1β proteins obviously down-regulated in the EA group ( P <0.05). Immunoflorescence staining showed that the number of GFAP-positive cells was apparently increased in the model group compared with the sham operation group and observably decreased in the EA group relevant to the model group ( P <0.05)., Conclusion: EA can significantly improve locomotor function in SCI mice, which is associated with its effects in reducing inflammation, oxi-dative stress reactions and reactive astrocyte proliferation via up-regulating expression of ApoE, p-ERK1/2, and Nrf2/HO-1 (antioxidant pathway) and inhibiting IL-1β and NF-κB expression.

Objective: To observe the effect of electroacupuncture (EA) combined with transplantation of Schwann cells (SCs) on limb locomotor, myelin sheath repair and expression of CD4 and CD8 in compressed spinal cord injury (CSCI) rats, so as to explore its mechanisms underlying improvement of CSCI., Methods: A total of 45 female SD rats were randomly divided into normal control, model, EA, Schwann cell (SC) transplantation, and EA＋SC transplantation groups ( n ＝9 rats in each group). The CSCI model was established by laminectomy at T12-L2 and clip compression. Rats of the SC transplantation group accepted injection of the cultured SC suspension (2×10 6 /6 µL) into the central, upper and lower sites of the injured spinal cord (5 mm in depth) 7-8 days after CSCI modeling. EA (2 Hz) was applied to bilateral "Zusanli" (ST36) and "Sanyinjiao" (SP6) for 10 min, once daily and 6 days a week for 3 weeks. The Basso, Beattie and Bresnahan locomotor rating scale (BBB scale) was used to evaluate the function state of CSCI. Morphological changes of the regional injured tissue were observed under light microscope after H．E. staining. The myelin sheath repair state and survival of SCs were detected by Luxol fast blue (LFB) staining and immunofluorescence histochemistry, and the expression of CD4, CD8 and P0 of the injured spinal cord was detected by Western blot., Results: Compared with the normal control group, the BBB scores at the time-points of 0 d, and 1, 2, and 3 weeks were significantly decreased in the model group ( P <0.001), and those of the EA＋SC transplantation group at the 2 nd and 3 rd week were significantly higher than those of the model group ( P <0.05). No significant changes of BBB scores were found after EA and SC transplantation relevant to the model group ( P >0.05). LFB staining showed a disordered arrangement of the nerve fibers in the white matter, myelinociasis and obvious decrease of the medullated fibers in the model group, and these situations were relatively milder in both EA and SC transplantation groups and obviously milder in the EA＋SC transplantation group. H．E. staining displayed that the structure of the injured region of the spinal cord was incomplete, accompanied with a large number of defect cavities and neuronal karyopyknosis in the model group, while the structure was relatively clear, with an increase of the normal neurons and fewer neuronal karyopyknosis in the EA＋SC transplantation group. Compared with the normal control group, MBP in the model group was significantly decreased ( P <0.001)，and P0 was significantly increased ( P <0.001). Compared with the model group, the expressions of MBP and P0 were significantly increased in the EA, SC transplantation, and EA＋SC transplantation groups ( P <0.01, P <0.001), and was significantly higher in the EA＋SC transplantation group than in both EA and SC transplantation groups ( P <0.001). The average immunofluorescence intensity of Hoechst33342-labeled SCs was significantly higher in the EA＋SC transplantation group than in the SC transplantation group ( P <0.05). After CSCI, the expression levels of spinal CD4, CD8 and P0 proteins had no significant changes in comparison with the normal control group ( P >0.05), while after the intervention and in comparison with the model group, the expression levels of P0 protein were significantly increased in the EA, SC transplantation and EA＋SC transplantation groups ( P <0.05), and was significantly higher in the EA＋SC transplantation group than in both EA and SC transplantation groups ( P <0.05). The expression levels of CD4 and CD8 proteins were significantly lower in the EA＋SC transplantation group than in the SC transplantation group ( P <0.05)．., Conclusion: EA＋SCs transplantation can improve the locomotor function in CSCI rats, which may be related to its effects in increasing the survival of transplanted SCs to promote the remyelination and in reducing the immune rejecting reaction.

Objective: To observe the therapeutic effect of electroacupuncture (EA) of "Zusanli" (ST36) and " Ashi "-point on the healthy side (opposing needling) on muscular injury and expression of myogenin (myoG) and fast myosin skeletal heavy chain (Fast MyHC) proteins in the gastrocnemius muscle (GM) tissues in skeletal muscle contusion rats，so as to explore its mechanism underlying improvement of skeletal muscle injury., Methods: A total of 54 male SD rats were divided into normal control (n ＝ 6)，model ( n ＝24) and opposing needling (EA, n ＝24) groups. The latter two groups were further randomized into 3, 5, 7 and 14 d subgroups ( n ＝6 per subgroup). The skeletal muscle contusion model of the hind-limb was established by using a self-made striking device. EA (1 Hz/3 Hz，1－2 mA) was applied to ST36 and " Ashi "-point on the uninjured side of the hind-limb for 15 min every time, once a day for 3, 5, 7 and 14 days, respectively. The injured GM was harvested on the 3 rd, 5 th, 7 th and 14 th day after muscular contusion. The morphological changes of the injured GM and the mean cross-sectional areas (CSAs) of the neonatal muscle cells were observed by microscope after H．E. staining. The immunoactivity of desmin protein (myogenic marker protein of myoblast cell) of GM was detected by immunofluorescence stain on the 7 th day after injury, and the expression levels of myoG (on the 3 rd and 5 th day after injury) and fast MyHC protein of GM tissues (on the 7 th and 14 th day after injury) were detected by Western blot., Results: H．E. staining of GS tissue showed fewer neuronal myocytes with disordered arrangement at different sizes, and appearance of some collagenous fibers among the mesenchyme on day 7 and 14 after muscular contusion, which was relatively milder in the EA group. In the EA group, the CSA values of the neonatal muscle cells were significantly larger than those in the model group on the day 7 th ( P <0.05), 14 th ( P <0.001) after injury. On day 7 after muscular contusion, the desmin was found to express on the cellular membrane of GM in the normal control group, while in the model group, the desmin expressed mainly in the cellular plasma in the model group, and on the cellular membrane of neonatal myocytes in the EA group, respectively. The desmin positive myocytes showed disordered arrangement and different sizes after muscular contusion, whereas the situations of the EA group were close to those of the normal control group. Desmin expression was up-regulated in the EA group compared with the model group which was not significant difference ( P >0.05). On the 3 rd and 5 th day after muscular contusion, the expression level of myoG protein was significantly up-regulated in the model group compared with the normal control group ( P <0.001), and significantly up-regulated in the EA group than that in the model group ( P <0.001). On the 7 th and14 th day after contusion, the expression level of fast MyHC protein was significantly down-regulated in the model group relevant to the normal control group ( P <0.001), and markedly up-regulated in the EA group relevant to the model group ( P <0.01)．., Conclusion: EA of ST36 and " Ashi "-point on the contralateral limb can up-regulate the expression of myoG and fast MyHC proteins of GM in acute skeletal muscle contusion rats, which may contribute to its effect in promoting the repair of skeletal muscle injury.

Objective: To observe the effect of electroacupuncture (EA) on morphological changes of denervated gastrocnemius(GS) and the expression of fork-head protein(FOXO3A), muscle atrophy F-box(MAFbx)and myogenic differentiation antigen (Myod1) in sciatic nerve injury rats, so as to reveal its mechanism underlying improvement of myoatrophy., Methods: Eighteen male Sprague-Dawley rats were randomly divided into sham operation, model and EA groups ( n ＝6 per group). The model of gastrocnemius atrophy was established by crushing the right sciatic nerve. Then, EA (2 Hz) was applied to the right "Zusanli" (ST36) and "Huantiao" (GB30) for 10 min, once a day for 14 successive days. The wet weight of the GS on both sides was weighted to calculate the wet weight ratio (the injured side /the healthy side), and the cross-sectional area (CSA) and diameter of GS fibers were measured after H．E. staining. The expressions of FOXO3A, MAFbx and Myod1 protein and mRNA in the GS tissue were tested using Western blot and fluorescence quantitative PCR, separately., Results: Following modeling, the GS wet weight ratio, CSA and fiber diameter were smaller in the model group than those in the sham group ( P <0.01), and were significantly higher in the EA group than in the model group ( P <0.01). H．E. staining showed that the GS fibers became smaller and the myocyte got round in the model group, while the GS fibers were bigger and the myocyte was relatively regular in morphology in the EA group. After modeling, the expression levels of FOXO3A, MAFbx and Myod1 mRNA and protein were evidently higher in the model group ( P <0.01); Moreover, after EA treatment, modeling-induced increasing of expression levels of FOXO3A and MAFbx mRNA and protein were revised ( P <0.01), while the increased expression level of Myod1 was further up-regulated relavant to that in the model group ( P <0.01)．., Conclusion: EA of ST36 and GB30 can suppress the up-regulated expression of FOXO3A and MAFbx mRNA and protein and further promote the expression of Myod1 mRNA and protein in the GS tissue in rats with denervated GS atrophy, which may contribute to its function in relieving the myoatrophy, promoting the skeletal muscle protein hydrolysis and differentiation of satellite cells.

Objective: To observe the effect of "Neiguan" (PC6)-electroacupuncture (EA) preconditioning on serum metabolites in myocardial ischemia-reperfusion injury (MIRI) rats, so as to reveal its mechanism underlying improvement of ischemic myocardium from metabonomics., Methods: A total of 48 male SD rats were randomly divided into control, model, EA "Neiguan"(PC6) and EA "Hegu"(LI4) groups ( n ＝12 rats/ group). Rats of the control group were just banded on animal boards for 30 min, once daily for 7 days. The MIRI model was established by occlusion of the left anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 1 h, and rats of the model group were also banded as those in the control group. Before modeling, EA (10 Hz/50 Hz, 1 mA) was applied to bilateral "Neiguan"(PC6) and "Hegu"(LI4) for 30 min, once daily for 7 successive days. After the treatment, serum samples were collected to be analyzed by proton nuclear magnetic resonance ( 1 H NMR) spectroscopy. The orthogonal partial least squares discriminate analysis (PLS-DA) was employed to distinguish the serum differential metabolic profile of rats in different groups and identify potential biomarkers., Results: After modeling, the ECG of model group and electroacupuncture groups showed T wave towering, and there was no obvious ST segment between R wave and T wave. The T wave decreased more than 0.2 mV after reperfusion, and there was no obvious ST segment. Compared with the control group, MIRI induced significant changes of metabolites in the serum including increase of acetoacetate acid, lectic acid, creatine, glycerol and glucose, and decrease of alanine, glutamine, glycerophosphoryl choline and phosphorylcholine. In comparison with the model group, PC6-EA preconditioning induced significant changes, including an increase of glucose, and a decrease of leucine，isoleucine, valine，3-hydroxybutyric acid，lactate，acetate，acetone，acetoacetate acid，pyruvic acid，glutamine，creatine and glycerol. There is no significant difference in metabolic patterns between "Hegu" group and model group. Metabolic pathway enrichment analysis indicated that the protective effect of PC6-EA pretreatment was realized mainly by regulating pathways of glycolysis, gluconeogenesis, citric acid metabolism, pyruvate metabolism, ketone body metabolism, etc., Conclusion: PC6-EA pretreatment has a role in regulating gluconeogenesis, pyruvate metabolism, amino metabolism, ketone body metabolism and energy metabolism in rats with MIRI, which maybe contribute to its protective effect on ischemic myocardium, but the specific metabolic pathways and mechanisms need being studied further.

Objective: To observe the effect of "Neiguan" (PC6)-electroacupuncture (EA) or moxibustion (Moxi) pretreatment on myocardial apoptosis and expression of autophagy related proteins light chain (LC) 3-Ⅰ and LC3-Ⅱ in rats with myocardial ischemia/reperfusion injury (MIRI), so as to explore their mechanisms underlying improvement of MIRI., Methods: Forty SD rats (half male and half female) were randomly divided into sham operation, model, ischemic preconditioning (IP), EA and Moxi groups ( n ＝8 in each group). EA (10 Hz/50 Hz, 1 mA) or Moxi (ignited moxa stick) was respectively applied to bilateral "Neiguan" (PC6) for 20 min, once daily for 7 days. The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 60 min. The ultrastructural changes and autophagy of myocardial cells were observed by electron microscopy (EM), and the myocardial cellular apoptosis [apoptotic index ＝ (number of apoptotic cells/total number of cardiomyocytes)×100%] was detected by the terminal deoxyribonucleotidyl transferase mediated dUTP nick end labelling (TUNEL) method. The expressions of LC3-Ⅰ and LC3-Ⅱ proteins (markers for autophagy) in myocardial tissue were detected by Western blot., Results: Following MI, EM observation revealed a vague structure of cardiomyocytes and muscular horizontal grain, dissolution of myofibers, mitochondrial swelling, some autophagic vacuoles and autophagic lysosomes at different degrees and surrounded by a double membrane in the model group, these situations were apparently milder in the EA and Moxi groups. The apoptosis index, myocardial LC3-Ⅰ and LC3-Ⅱ protein expression levels, and the ratio of LC3-Ⅱ/Ⅰ were significantly increased in the model group relevant to the sham operation group ( P <0.05). After the treatment, the apoptosis index, the expression level of myocardial LC3-Ⅱ protein and the ratio of LC3-Ⅱ/Ⅰ were considerably down-regulated in the IP, EA and Moxi groups in comparison with those in the model group ( P <0.05). The effect of EA was obviously superior to those of IP and Moxi in down-regulating the apoptosis index ( P <0.05), but obviously inferior to those of IP and Moxi in down-regulating the levels of LC3-Ⅱ and LC3-Ⅱ/Ⅰ ( P <0.05). No significant changes were found in the expression of LC3-Ⅰ after IP, EA and Moxi interventions in comparison with the model group ( P >0.05), and no significant differences were observed in the apoptosis index and levels of LC3-Ⅱ and LC3-Ⅱ/Ⅰ between the IP and Moxi groups ( P >0.05)．., Conclusion: Both EA and moxibustion pretreatments, similar to IP, have a positive role in reducing myocardiocyte apoptosis and regulating autophagy-related protein expression in MIRI rats, which maybe contribute to their protective effects on ischemic myocardium.

Objective: To explore the effect of electroacupuncture(EA) on amyotrophia and expression of paired box7(Pax7), myogenic differentiation antigen-1 (Myod1), myogenin (Myog), myosin heavy chain- Ⅱa (Myh2), myosin heavy chain-Ⅱx (Myh1) and myosin heavy chain-Ⅰ (Myh7) of denervated gastrocnemius in rats with chronic constriction injury (CCI) of the right sciatic nerve, so as to explore its mechanisms underlying postponing development of amyotrophia., Methods: Sixty-six SD adult male rats were randomly divided into sham operation (sham) group ( n ＝24), model group ( n ＝24) and EA group ( n ＝18). The denervated muscle (gastrocnemius) atrophy model was established by CCI of the right sciatic nerve. EA (2 Hz，1.0 mA) was applied to the right "Zusanli"(ST36) and "Huantiao"(GB30) for 10 min, once a day, six times a week and for 1, 2 and 4 weeks, respectively. After complete dissection of bilateral gastrocnemius muscles, their wet weight levels were measured and the ratio of wet weight (＝that of the operation side/that of the non-operation side) was calculated, and the cross-sectional area (CSA) and diameter of the gastronemius were detected after fixation in 4% paraformaldehyde, sectioning, and H．E. staining. The expression levels of Pax7, Myod1, Myog, Myh2, Myh1 and Myh7 mRNAs in the gastrocnemius tissue after 3 weeks of modeling were detected with quantitative real time-PCR (qPCR)., Results: After 1 week of modeling, the ratios of wet weight of gastrocnemius and the CSA and fiber diameter at the 2 nd , 3 rd and 5 th week were significantly smaller in the model group than in the sham group ( P <0.05). The expression levels of Myod1 and Myog mRNAs were significantly up-regulated ( P <0.01), and those of Myh2, Myh1 and Myh7 considerably down-regulated in the model group compared with the sham group ( P <0.05, P <0.01). No significant changes were found in the expression levels of Pax7 mRNA after modeling and EA intervention ( P >0.05)．Following EA intervention, the CSA and diameterof the gastronemius were obviously increased ( P <0.05), and the expression levels of Myod1, Myog and Myh7 further markedly or remarkably up-regulated in comparison with the model group ( P <0.05, P <0.01). No significant changes were found in the ratio of wet weight of gastrocnemius at the 3 time-points, and the expression levels of Myh2 mRNA and Myh1 mRNA in the EA group relevant to the model group after 3 weeks of modeling ( P >0.05)., Conclusion: EA treatment may delay gastrocnemius atrophy in CCI rats, which is possibly associated with its effects in up-regulating the expression of Myod1, Myog and Myh7 mRNAs to control the differentiation of the satellite cells and the muscle fiber type transformation.

Objective: To explore the effect of electroacupuncture (EA) on the expression of synovial AMP-activated protein kinase (AMPK) protein α， arginase-1 mRNA, nitric oxide synthase 2 (NOS 2) mRNA, NOD-like receptor protein 3 (NLRP 3) mRNA, and interleukin-1 β (IL-1 β) mRNA in acute gouty arthritis (AGA) rats, so as to explore its mechanisms underlying improvement of AGA via M 1/M 2 macrophage polarization., Methods: Male Wistar rats were randomly divided into normal control, model, medication (colchicine) and EA groups ( n ＝15 rats in each group). The AGA model was established by injection of sodium urate crystal (MSU) suspension (0.2 mL) into the articular cavity of the left knee. The rats of the normal control group received articular injection of normal saline (0.2 mL) of the left knee, and those of the medication group were treated by gavage of the colchicine (0.3 mg•kg －1 •d －1 ) once daily for 7 days. EA (2 Hz/10 Hz, 1.0 mA) was applied to "Zusanli"(ST 36) and "Sanyinjiao" (SP 6) of the left hind limb for 10 min, once daily for 7 days. The inflammatory conditions of the synovial membrane tissue of the left knee joint were observed by H．E. staining. The expression levels of phosphorylated AMPKα (p-AMPKα) protein, and arginase-1 (a maker of M 2 macrophages) mRNA, NOS 2 (a maker of M 1 macrophages) mRNA, NLRP 3 mRNA, and IL-1 β mRNA in the knee joint synovial tissue were detected by Western blot and quantitative real-time PCR, respectively., Results: Compared with the normal group, the inflammatory cell infiltration of the synovial tissue was more severe, the expression of p-AMPKα protein was significantly decreased ( P <0.01), and the expression levels of arginase-1, NOS 2, IL-1 β and NLRP 3 mRNAs were considerably increased in the model group ( P <0.01). The expression levels of p-AMPKα protein and arginase-1 mRNA were significantly up-regulated, and those of NOS 2, IL-1 β and NLRP 3 mRNAs obviously down-regulated in both EA and medication groups relevant to the model group ( P <0.01, P <0.05), suggesting an increase of M 2 macrophage and a decrease of M 1 macrophage activity after EA. No significant differences were found between the EA and medication groups in up-regulating p-AMPKα expression and in down-regulating NOS 2, IL-1 β and NLRP 3 mRNA expression ( P >0.05), except higher up-regulation of arginase-1 mRNA in the medication group ( P <0.05)．., Conclusion: EA intervention can up-regulate the expression of arginase-1 mRNA and p-AMPKα protein, and down-regulate the expression of NOS 2, IL-1 β and NLRP 3 mRNAs in synovial tissues in AGA rats, which may contribute to its anti-inflammatory effect by promoting conversion of macrophages from M 1 pro-inflammatory phenotype to M 2 anti-inflammatory phenotype.

Objective: We have repeatedly demonstrated that electroacupuncture (EA) of "Neiguan"(PC 6) can improve myocardial ischemia in rats. The present study was designed to investigate the metabolomic profile of peripheral blood se-rum and myocardium involving EA-induced improvement of myocardial ischemia-reperfusion injury (MIRI) in rats by using nuclear magnetic resonance spectroscopy., Methods: Thirty male SD rats were equally randomized into blank control, model and EA groups. Rats of the control group were only banded for 20 min, once a day for 7 days. The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 60 min, and rats of the model group were banded as those in the control group. EA (10 Hz/50 Hz, 1 mA) was applied to bilateral PC 6 for 20 min, once daily for 7 days. The blood samples and left ventricular myocardial tissues were collected for assaying the profiles of differential metabolites using 1 H nuclear magnetic resonance ( 1 H NMR) spectroscopy and multivariate statistical analysis such as the principal components analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) with SIMCA-P software 12.0., Results: A total of 19 differential metabolites (17 down-regulated, 2 up-regulated) in the serum and 14 differential metabolites (13 down-regulated and 1 up-regulated) in the ischemic left myocardium were identified after MIRI. Of the 19 serum differential metabolites, amino acids (leucine, isoleucine, valine,alanine, lysine, glycine, glutamine), 3-hydroxy butyric acid (3-HB), lactic acid, acetate, N-acetyl glycoprotein (NAc), acetone, acetoacetate, succinate, polyunsaturated fatty acids (PUFA), creatine, glycerophosphocholine (GPC) were down-regulated; while low density lipoprotein (LDL), LDL/very low density lipoprotein(LDL/VLDL)and glucose obviously up-regulated. Of the 14 myocardial differential metabolites, amino acids (alanine, lysine, glutamate, glutamine, aspartate, taurine, glycine, threonine), GPC, creatine, lactic acid, adenosine monophosphate (AMP), nicotinamide adenine dinucleotide (NAD＋) were significantly decreased, and glucose was up-regulated. Following EA treatment, most of the decreased serum differential metabolites except acetone, acetoacetate and PUFA, and the increased serum LDL, LDL/VLDL and glucose recovered, basically close to the control level; and the decreased myocardial creatine, GPC and NAD＋ were also apparently up-regulated and the increased myocardial glucose was down-regulated. But, myocardial threonine and AMP still presented a decreasing state. Although the pattern of myocardial differential metabolites of the EA group had a trend to be close to the control group, the significant difference still existed, while the metabolic pattern of serum metabolites in the EA group was close to that of the control group., Conclusion: EA stimulation of PC 6 can regulate serum or/and myocardial metabolites as amino acids, carbohydrates, lipids, etc. in MIRI rats, of which both serum and myocardial creatine, GPC and glucose may be jointly confer a favorable potential for EA-induced improvement of MIRI.

Objective: To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) pretreatment on myocardial pathological and structural changes and expression of autophagy related protein LC 3 Ⅰ/Ⅱ and Beclin 1 in rats with myocardial ischemia-reperfusion injury (MI/RI), so as to explore their mechanisms underlying improving MI/RI., Methods: Forty SD rats were randomly divided into sham operation, model, ischemic preconditioning (IP), EA and Moxi groups ( n ＝8 in each group). EA (10 Hz/50 Hz，1 mA) or Moxi (ignited moxa stick) was respectively applied to bilateral "Neiguan"(PC 6) for 20 min, once daily for 7 days. The MI/RI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 60 min. The left ventricular (LV) tissue samples were collected and analyzed for pathological (H.E. staining) and ultrastructural changes, for myocardial apoptosis (apoptotic index＝ number of apoptotic cells/total number of cardiomyocytes×100%) with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method, and for the expression of LC 3 and Beclin 1 in myocardial cells with Western blot., Results: Following MI/RI, H．E. staining revealed a disorder of arrangement of cardiomyocytes with vague border, inflammatory cell infiltration, intracellular swelling with bleeding, necrosis and dissolution of partial striated muscles of the left ventricle under light microscope, and dual staining of Uranyl acetate and leadnitrate showed atrophy, arrangement disorder, dissolution, necrosis, and interstitial edema of partial myocardial fibers, mitochondrial structural disorder, vacolation, and large body of autophagosomes with bilayers, etc. in ultrastructure, which was relatively lighter in both EA and Moxi groups. The apoptosis index, expression levels of myocardial LC 3 Ⅱ and Beclin 1 and the ratio of LC 3 Ⅱ/LC 3 Ⅰ were significantly higher in the model group than those in the sham operation group ( P <0.01), but the expression level of LC 3 Ⅰ was considerably down-regulated in the model group relevant to the sham operation group ( P <0.01). Following the intervention and MI preconditioning, the increased apoptosis index and expression levels of LC 3Ⅱ and Beclin 1 proteins and the ratio of LC 3Ⅱ/LC 3 Ⅰ were obviously down-regulated in the IP, EA and Moxi groups relevant to the model group ( P <0.01), and the decreased expression of LC 3 Ⅰ protein was up-regulated obviously in the 3 treatment groups ( P <0.05， P <0.01). The effects of EA were significantly superior to those of IP and Moxi groups in down-regulating apoptosis index and expression of LC 3 Ⅱ and Beclin 1 and the ratio of LC 3 Ⅱ/LC 3 Ⅰ and in up-regulating expression of LC 3 Ⅰ ( P <0.05, P <0.01)., Conclusion: Both EA and Moxi preconditioning of PC 6 have a protective effect on ischemic myocardium in MI/RI rats, which is probably related to their effects in regulating expression of myocardial autophagy proteins as LC 3 Ⅰ/Ⅱ and Beclin 1.

To explore the relativity between heart-brain and post-stroke anxiety disorder by analyzing the pathogenesis of post-stroke anxiety disorder combined with the physiology and pathology of organs. The acupoint selection thought was explored by its selection rule according to literature. Post-stroke anxiety belongs to the brain organic anxiety disorder, whose basic pathogenesis is mind injury. The crucial cause is heart and brain disorder at the same time. Thus, Baihui (GV 20), Fengfu (GV 16) for brain and Xinshu (BL 15), Shenmen (HT 7) for heart can improve anxiety disorder according to the theories such as five organ, qijie ( qi thoroughfare), sihai (four seas), meridian and collateral.

Objective: To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) pretreatment on expression of myocardial heat shock protein (HSP) in acute myocardial ischemia-reperfusion injury (MIRI) rabbits., Methods: A total of 72 New Zealand rabbits were randomly divided into 4 groups:sham operation, MIRI model, EA pretreatment and Moxi pretreatment ( n =18 rabbits in each group) which were further divided into 0, 24 and 48 h (time-point) subgroups ( n =6 in each). The MIRI model was established by occlusion of the anterior descending branch (ADB) of the left coronary artery for 40 min and reperfusion for 60 min. EA and Moxi stimulation was respectively applied to bilateral "Neiguan"(PC 6) for 20 min, once daily for 5 days before ADB occlusion. The expressions of myocardial HSP 27, HSP 70 and HSP 90 were detected by immunohistochemistry. The pathological and ultrastructural changes of left ventricular ischemia tissue were observed under light and transmission electronic microscope (TEM), respectively., Results: Outcomes of H.E. staining and ultrastructure showed that MIRI-induced changes of disordered arrangement of cardiomyocytes, vague myocardial transverse striation, inflammatory infiltration, cardiac myofibre necrosis and fibrolysis (light microscope), and myofiber atrophy, vague and disorder in the arrangement of myofiber, myofilament necrosis, interstitial edema, mitochondrial swelling, microvessel expansion, etc. (TEM) were relatively milder in both EA and Moxi pretreatment groups (48 h). In comparison with the sham group, the expression levels of myocardial HSP 27, HSP 70 and HSP 90 had no significant changes after MIRI at the 3 time-points ( P >0.05). In the pretreatment groups, the expression levels of HSP 27 at 24 and 48 h in both EA and Moxi groups, HSP 70 at 48 h in both groups, HSP 70 at 0 and 24 h in the Moxi group were significantly up-regulated compared with the model group ( P <0.05, P <0.01). No significant changes were found in the expression of HSP 90 at the 3 time-points in the EA and Moxi pretreatment groups ( P >0.05). No significant differences were found between EA and Moxi in up-regulating expressions of myocardial HSP 27, HSP 70 and HSP 90 proteins at the 3 time-points ( P >0.05) except HSP 70 at 24 h (Moxi being stronger relative to EA, P <0.05)., Conclusions: EA and Moxi pretreatment has a protective effect on ischemic myocardium in MIRI rabbits, which Feb be associated with their actions in up-regulating myocardial HSP 27 and HSP 70 expression.

Based on the MODIS-Aqua aerosol optical depth (AOD) products from 2003 to 2014, Nighttime Lights Time data from DMSP satellites and basic meteorological data, the AOD spatial distributions of interannual and seasonal variations over three northeastern provinces of China(Liaoning, Jilin, Heilongjiang) were analyzed. It was found that there was a northeast-southwest area of high annual average AOD composed of Dalian, Shenyang, Changchun, Harbin and other cities, the 12-year average AOD value was 0.4-0.8. The low AOD occurred in the eastern and northern areas of the three northeastern provinces of China, where the forest-covering rate was high, and the 12-year average AOD value was less than 0.3. The seasonal variations of annual average AOD showed an increasing trend from spring to summer, then decreased in autumn and increased again in winter. The interannual variations of AOD over three northeastern provinces of China showed a decreasing trend in most areas, but the increasing trend occurred in the northeast-southwest region with the axis formed by Shenyang, Changchun and Harbin, revealing the polarization in recent 10 years over three northeastern provinces of China. In addition, spatial distribution of annual average AOD over three northeastern provinces of China in the years of strong and weak Western North Pacific Summer Monsoon was studied. Affected by the surface wind field, annual average AOD in weak monsoon years was higher than that in strong monsoon years.

Objective: To compare the difference in the clinical efficacy on infantile food accumulation treated with centripetal tuina therapy at spleen meridian site of different location., Methods: Sixty cases of infantile food accumulation were randomized into a palmar thumb surface group and a radial margin group, 30 cases in each one. In the palmar thumb surface group, the spleen meridian was stimulated with the pushing technique centripetally on the palmar side of the thumb. In the radial margin group, the spleen meridian was stimulated with the pushing, technique centripetally along the radial margin of the thumb. The traditional tuina techniques, such as tuisanguan, yun neibagua were combined in the two groups. The treatment was given once a day, 6 times a week, 12 treatments as one session. After the 1 session of treatment, the main symptom scores before and after treatment were compared in the patients of the two groups, the improvements in the accompanied symptoms were observed, such as appetite, defecation and tongue coating; and the efficacy was evaluated in the two groups., Results: The total effective rates were 93.3% (28/30) and 90.0% (27/30) in the palmar thumb surface group and the radial margin group respectively and the difference was not significant statistically in comparison of the two groups (P > 0.05). After treatment, the main symptom score was improved significantly in the two groups, indicating the significant difference as compared with that before treatment (both P < 0.05). The improvement in foul breathing in the palmar thumb surface group was better than that in the radial margin group (P < 0.05)., Conclusion: Between the two kinds of pushing techniques at spleen meridian site, pushing on the palmar surface of the thumb and pushing along the radial margin, there is no difference in the total efficacy on infantile food accumulation. Regarding the improvement in foul breathing, the effect in the palmar thumb surface group is better than that in the radial margin group.

For the treatment of primary dysmenorrhea, professor ZHAO Jiping focuses on meridian diagnosis and inspection, and uses pressing methods to locate the response points along the meridian, including acupoints and aishi points. During the stage of attack, it is essential to press along the spleen meridian, mainly Sanyinjiao (SP 6), Diji (SP 8) and Yinlingquan (SP 9); during the stage of remission, it is essential to press along the bladder meridian and stomach meridian, mainly Ganshu (BL 18), Pishu (BL 20), Weishu (BL 21), Shenshu (BL 23) and Zusanli (ST 36). The differences between deficiency syndrome and excess syndrome lead to the different feelings of doctors and patients. Combined with the results of meridian diagnosis and inspection, the aim of treatment can be achieved by different acupuncture methods. Professor ZHAO pays attention to the treatment of accompanied symptoms and timing of treatment, since the relief of accompanied syndromes and selection of timing are keys to relieving patient's pain.

Objective: To observe the clinical efficacy and safety on moderate and severe persistent allergic rhinitis treated with acupuncture., Methods: Sixty-six patients of moderate and severe persistent allergic rhinitis were randomized into an acupuncture group (34 cases) and a western medication group (32 cases). In the acupuncture, group, acupuncture was applied to Dazhui (GV 14), Feishu (BL 13), Pishu (BL 20), Ganshu (BL 18) and Shenshu (BL 23) in the prone, retained for 20 min; then in the supine, at Baihui (GV 20), Yintang (GV 29), yingxiang (LI20) Taichong (LR 3) and Hegu (LI 4), retained for 20 min. Acupuncture was given once every two days, three times a week, continuously for 8 weeks. In the western medication group, cetirizine hydrochloride was taken orally, 10 mg each time, once every day, continuously for 8 weeks. Separately, before treatment, after the treatment of 1 and 2 months and in 1 month after treatment, the total nasal symptom score (TNSS), the scores in the emotion rating scale for Ganzangxiang of TCM (ERSG) and the rhinoconjunctivitis quality of life questionnaire (RQLQ) were observed in the patients of the two groups. The clinical efficacy was compared between the two groups., Results: (1) For TNSS, the results after 1 and 2 months treatment and in 1 month after treatment were all, reduced as compare with that before treatment separately in the two groups (P < 0.05, P < 0.01) The result after 2 months treatment was lower than that after 1 month treatment in the acupuncture group (P < 0.05). In 1 month after treatment, the result in the acupuncture group was lower than that in the western medication group (P < 0.05). (2) For ERSG, the score after 2 months treatment was lower than that before treatment in the two groups (both P < 0.05). The score after 2 months treatment in the acupuncture group was lower than that in the western medication group (P < 0.05). (3) For RQLQ, the score after 1 month treatment was lower than that before treatment and the score after 2 months treatment was lower than that after 1 month treatment in the two groups (all P < 0.05). The score after 1 and 2 months treatment and in 1 month after treatment in the acupuncture group was lower than that in the western medication group separately (all P < 0.05). (4) The total effective rate was 91.2% (31/34) in the acupuncture group and was 90.6% (29/32) in the western medication group, without significant difference between the two groups (P > 0.05)., Conclusion: Acupuncture is the safe and effective intervention on moderate and severe persistent allergic rhinitis. Compared with the western medicine group, the efficacy in the acupuncture group presents much more advantageous at its durability.

Objective: To identify pre-operative demographic and urodynamic (UD) parameters related to overactive bladder (OAB) symptoms of pre-operation and persistence of after pelvic organ prolapse (POP) repair., Methods: From Jan 1, 2010 to Oct 31, 2012, this perspective study examined demographic and UD data of 175 patients undergoing POP surgery. Pre- and post- operative urinary distress inventory 6 (UDI- 6) scores for frequency and urge urinary incontinence (UUI) were analyzed, and correlations between scores and pre-operative UD data were also analyzed., Results: (1)Surgery resulted in a improvement of frequency (71.0% , 71/100) and UUI (69.2% , 63/91) at 12-24 months follow-up. (2)Pre-operative UD parameters: first desire of bladder <100 ml, 6.3% (11/175); detrusor overactivity, 14.9% (26/175); post-void residual ≥50 ml, 11.4% (20/175); maximal flow rate (Qmax), (16±7) ml/s. (3)Comparison of pre- operative UD datas between patients with OAB symptoms and without, with OAB symptoms group had more patients of type III stress urinary incontinence [5% (5/91) versus 0 (0/84), P = 0.022]. (4)Qmax was higher in improvement in UUI group than that in persistent UUI after POP repair [(17±7) ml/s versus (13±5) ml/s, P < 0.01]., Conclusions: POP repair significantly reduces OAB symptoms; however, there had not exact predictor for symptoms of persistent frequency, UUI after POP repair. Persistent UUI symptom may be related to Qmax of pre-operative UD.

Objective: Decolorization of crude chitin from Catharsius molossus was studied in order to prepare chitosan with white color and high viscosity., Methods: Some factors affecting decolorization such as the kind of decolorant reagent, H2O2 concentration, pH, solid-liquid ratio, temperature and other factors were studied by single factor test and orthogonal design., Results: The best decolorization process of chitin from Catharsius molossus was as follows:treated with 15 times of 15% H2O2 at 45 degrees C., Conclusion: The process is stable and feasible. The chitosan from Catharsius molossus might be used as a new carrier of colon-specific drug delivery system.

Objective: To explore the independent risk factors and predictive factors of cognitive deterioration in patients of vascular cognitive impairment no dementia with subcortical ischemic vascular disease (VCIND-SIVD)., Methods: The clinical and neuropsychological characteristics of 96 VCIND-SIVD patients were collected and their cognitive states re-assessed after one year. The distribution of continuous and categorical variables was examined with univariate analysis. And Logistic regression in multivariate modeling was employed to identify the risk and predictive factors of cognitive deterioration., Results: After 1-year follow-up, 8 patients dropped out, 2 died and the remainder remained. There were 48 males and 38 females with an average age of 64.3 ± 5.4 years. Among them, 17 (19.8%) deteriorated into dementia, 24 (27.9%) upgraded to normal cognition and 45 (52.3%) maintained their cognitive status. They were divided into dementia group (cognition deteriorating into dementia) and control group (elevated/sustained cognition). After univariate analysis of general information and vascular risk factors, Logistic regression demonstrated that education level (P < 0.05, OR = 0.7) was a preventive factor of dementia while age (P < 0.05, OR = 1.2), hypertension (P < 0.05, OR = 5.6) and high homocysteine (P < 0.05, OR = 4.2) were independent risk factors of dementia. After univariate analysis of neuropsychological scores, Logistic regression demonstrated that visual reproduction-immediate recall (P < 0.05, OR = 3.4), digit span forward (P < 0.05, OR = 1.2), clock drawing test (P < 0.05, OR = 6.8) and completion time of London tower (P < 0.05, OR = 5.4) were predictive factors of dementia., Conclusion: VCIND-SIVD patients with low score of visual reproduction-immediate recall, digit span forward, clock drawing test and completion time of London tower should receive vigorous controls of hypertension and high homocysteine.

Objective: To explore correlation between lung and large intestine and the two meridians under pathological condition in the view of meridian theory., Methods: Ninety-six cases of bronchial asthma were applied palpation at the running course of 12 regular meridians under the elbow and knees and back-shu points. And abnormal reactions were recorded, the affected meridians and back-shu points were discovered., Results: The abnormal reactions most frequently appeared on the Lung Meridian, followed by the Large Intestine Meridian, the Spleen Meridian, the Liver Meridian, the Stomach Meridian and the Triple Energizer Meridian. And the unusual reaction of the back-shu points most frequently appeared on Feishu (BL 13), and Dachangshu (BL 25) and Pishu (BL 21) followed as the next two., Conclusion: The existence of correlation between the Lung Meridian and the Large Intestine Meridians under pathological condition can be proved through meridian and acupoint palpation on bronchial asthma patients.

Objective: To observe the improvement of the quality of life (QOL) of patients with bronchial asthma treated with acupuncture based on the interior-exterior relationship of the lung and large intestine, in terms of meridian-collateral theory and through the lung and large intestine meridians., Methods: According to the random number table, 52 cases of bronchial asthma at chronic persistent stage of the middle degree in accordance with the inclusive criteria were divided into a lung meridian acupoint group (group A), a large intestine meridian acupoint group (group B), a lung and large intestine meridian acupoint group (group C) and a control group at the ratio of 1:1:1 = 1, 13 cases in each one. In the control group, according to the requirement, Ventolin aerosol was used, one spray/time, < or = 4 times/day. The treatment of 12 weeks was required. In the first 3 groups, on the basis of the treatment as the control group, acupuncture was supplemented. In group A, Chize (LU 5), Kongzui (LU 6), Lieque (LU 7), etc. were selected. In group B, Quchi (LI 11), Hegu (LI 4), Tianshu (ST 25), etc. were selected. In group C, Chize (LU 5), Kongzui (LU 6), Lieque (LU 7), Quchi (LI 11), Hegu (LI4), etc. were selected. Acupuncture was given once every two days, 3 times a week. Totally, 12 treatments made one session and 3 sessions (12 weeks) were required. The Asthma Quality of Life Questionnaire (AQLQ) was adopted to assess the total score and four subscales, named activity limitation, environment stimulation, emotion and symptom, and determine the efficacy., Results: In comparison before and after treatment in the same group, AQLQ total score, emotion score and symptom score were all increased significantly in group A and group C separately (P < 0.05, P < 0.01). The environment stimulation score was increased in group C (P < 0.05). In group B, AQLQ total score and the activity score were increased remarkably (both P < 0.05). In comparison among groups: compared with the control group, AQLQ total score and symptom score in group A and group C were improved obviously (P < 0.05, P < 0.01); the emotion score was increased obviously in group B and group C (both P < 0.01) and the environment stimulation score was increased obviously in group C (P < 0.05). Compared with group B, the symptom score was improved much obviously in group C (P < 0.05)., Conclusion: Acupuncture improves the quality of life much more obviously for the patients with bronchial asthma in terms of the lung and large intestine theory.

Objective: To study preparation and characterization of chitosan from Catharsius Molossus processing discards and explore the feasibility of utilization of insects residue., Methods: Preparation technique of chitosan was studied by orthogonal design,and properties were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), etc., Results: Preparation techniques were as follows: demineralizing: soaked for 30 min at 80 degrees C with 1.3 mol/L HCl, then kept for 12 h under room temperature. Deproteinization and delipidation: treated for 6 h at 90 degrees C with 4 mol/L NaOH. Decolorizing: soaked at room temperature with 3% KM-nO4, then treated with 2% oxalic acid at 70 degrees C. Deacetylating:treated for 5 h at 110 degrees C with 14 mol/L NaOH., Conclusion: The technique is stable and feasible. The result also preliminarily showes that chitosan from Catharsius Molossus is better than shrimp's. It will be widely applied in biomedical and other industrial areas with such exciting properties.

In the present paper, the authors review the development of experimental and clinical studies on acupuncture treatment of bronchial asthma in recent 10 years. Regarding clinical studies, results showed that acupuncture could (1) regulate cardiac-pulmonary function; and (2) adjust immune state and relieve inflammatory reactions in bronchial asthma patients. Animal experiments showed that acupuncture could function in (1) improving pulmonary function; (2) reducing accumulation of the peripheral eosinophile granulocytes (EOS), relieving the infiltration of inflammatory cells in the air-passage mucosa and promoting the apoptosis of EOS in the lung and air-passages; (3) down-regulating the expression of air-passage remodeling-related protein insulin growth factor-1; (4) suppressing the secretion of tumor necrosis factor and endothelin; (5) attenuating allergic reaction; (6) regulating neuroendocrine activity; and (7) modulating intracellular second messenger activities. However, rigorous clinical study design is not enough, so that the reliability of the results is limited. In spite of many indicators of animal experiments have been selected, but their correlations are not in close association, resulting in poor complementation and mutual identification of the acquired findings. For this reason, its clinical efficacies need to be researched further according to principles of evidence-based medicine.

Objective: To assess the clinical significance of three different noninvasive airway inflammatory indices in induced sputum and exhaled breath condensate (EBC) from persistent asthmatic patients., Methods: Moderate and severe asthmatic patients were prescribed inhaled corticosteroids combined with long-acting beta(2) agonists for a month. The symptom scores and percentage of predicted value of forced expiratory volume in one second (FEV(1)) (FEV(1)%pred) were measured while the concentrations of H(2)O(2), NO(3)(-)/NO(2)(-), and cysteinyl-leukotriene E(4) (LTE(4)) in induced sputum and EBC were detected before and after therapy., Results: A total of twenty-five subjects with moderate and severe asthma were enrolled. By combined therapy for one month the asthma symptoms relieved and FEV(1)%pred improved significantly (P < 0.01). The concentrations of H(2)O(2), NO(3)(-)/NO(2)(-) and LTE(4) in induced sputum and EBC declined significantly (P < 0.01) although the concentrations were still higher than those at normal baseline. More marked reduction of H(2)O(2) and NO(3)(-)/NO(2)(-) compared to LTE(4) was observed. It was revealed that the concentrations of H(2)O(2)and NO(3)(-)/NO(2)(-) but not of LTE(4) in EBC were negatively correlated with FEV(1)%pred (P < 0.01) and positively with symptom scores. Such correlations were also found in H(2)O(2) in induced sputum with FEV(1)%pred and symptom scores as well as NO(3)(-)/NO(2)(-) in induced sputum with FEV(1)%pred. The improvement of FEV(1)%pred after treatment was positively correlated with the reduction of H(2)O(2) and NO(3)(-)/NO(2)(-) both in induced sputum and EBC. Correlation analysis also demonstrated three inflammatory indices were equivalent in induced sputum and EBC (correlation coefficient of H(2)O(2), NO(3)(-)/NO(2)(-) and LTE(4), 0.759, 0.826 and 0.653, respectively. P < 0.01)., Conclusions: (1) Combined therapy with inhaled corticosteroid plus long-acting beta(2) agonist significantly improves the clinical symptoms and lung function of patients with moderate and severe asthma companies with marked suppression of airway inflammation. (2) Both of EBC and induced sputum sampling are valuable noninvasive procedures for detecting asthma airway inflammation, however, EBC technique is superior in safety and reproducibility. (3) H(2)O(2) and NO(3)(-)/NO(2)(-) seem to be more sensitive indices in diagnosis and monitoring asthma compared to LTE(4).

Objective: To study the incidence rate, pattern and affective factors of mental disorder due to brain damage., Methods: According to CCMD-2-R, 388 subjects with traumatic brain damage in the Psychiatric Hospital of Huainan city within last 5 years were assessed by three psychiatrists 6 months to 1 year after brain injury., Results: (1) 74.2% of the mental disordered due to brain injury have intellectual impairment, most of them is mild; (2) The intracranial hematoma, brain stem injury, brain injury extent, GCS, complicated mental disorder, and education have great effect on intellectual impairment; (3) There is an intimate relationship between the intellectual impairment and the brain stem injury, intracranial hematoma, GCS, brain injury extent, and unconsciousness time. There is an intimate relationship between the mental symptom and the brain injury extent, contusion and laceration of brain, frontal lobe injury, and intracranial hematoma. There is also an intimate relationship between the personality change and the frontal lobe injury, unfolding brain case treatment, and intracranial hematoma., Conclusion: To assess overall mental disorder should rely on the characteristics of craniocerebral injuries.

Objective: To monitor the changes of symptom scores, airway hyperresponsiveness (AHR, represented by PC(35) sGaw), FEV(1)% and PEF% in patients with mild and moderate persistent asthma who received combined therapy of inhaled corticosteroids (ICS) and long-acting beta(2) agonists (LABA) and to evaluate the clinical significance of PC(35) sGaw and other parameters in guiding the adjustment of asthma stepwise therapy., Methods: Patients with asthma were allocated randomly to group A (22 subjects), B (22 subjects), and C (21 subjects). The initial regimens for all patients in the first three months included ICS (fluticasone) plus LABA (salmeterol). For patients in group A, a fixed dosage was maintained for 18 months, while those in group B received tailored dosage or withdrawal of therapy according to the clinical control level (well or total control). The regimens for patients in group C included step-down or withdrawal according to PC(35) sGaw besides the clinical control. All subjects were followed-up for 18 months and the symptom scores, PC(35) sGaw, FEV(1)% and PEF% were measured and analyzed. The asthma clinical control levels of the three groups at end point were compared., Results: A total of 65 subjects were enrolled and 46 completed the study. From the first to the third month after treatment, the symptom scores, FEV(1)% and PEF% improved significantly (t = 9.54, 13.17, 14.27, 12.4, 6.72, 6.59, 8.31, 5.22, and 5.96, respectively, all P < 0.01), and then maintained at relatively normal levels in a narrow range without significant progressive improvement during the later phases of the study. Meanwhile AHR declined abruptly in the first three months (t = 9.71, 12.04, and 14.31 in group A, B, C, respectively, all P < 0.01), followed by a slow but continuous improvement from the third to ninth month, and then maintained at a very low level. AHR disappeared in 4 cases but relapsed in 1 case after therapy withdrawal. The asthma clinical control level at the end point of group A, group B and group C were 93.3%, 53.3% and 93.8%, respectively (group A and group C versus group B, P < 0.01, respectively; group A versus group C, P > 0.05). There were fewer patients who underwent step-down therapy or withdrawal in group C compared to group B. However, patients in group C gained better asthma control and experienced less exacerbations compared to those in group B., Conclusions: (1) Combined therapy with ICS plus LABA significantly improves symptoms, lung function and AHR of asthmatic patients. (2) Adjustment of therapy based only on clinical parameters may lead to early step-down or withdrawal and therefore asthma exacerbations. (3) PC(35) sGaw, an index of AHR, may be valuable in assessing asthma severity, evaluating the efficacy of treatment and guiding medication adjustment.

Objective: To analyze the correlations between NO(3)(-)/NO(2)(-), eosinophil counts in induced sputum and airway hyperresponsiveness (AHR) and therefore to explore the clinical significance of these parameters in severity assessment and medication adjustment in patients with mild to moderate asthma., Methods: From February 2003 to June 2004, 35 outpatients with mild to moderate persistent asthma (mild: 9, moderate: 26) from Huaxi Hospital asthma clinic were treated with combined medications of inhaled corticosteroids (ICS) plus long-acting beta(2) agonist (LABA) for one year. The symptom scores were recorded, and AHR (represented by PC(35)sGaw), eosinophil counts and NO(3)(-)/NO(2)(-) concentrations in induced sputum were measured at regular intervals. Fifteen healthy volunteers served as control and eosinophil counts and NO(3)(-)/NO(2)(-) concentrations in induced sputum were measured., Results: A total of 35 subjects were enrolled, of whom 26 completed one-year or longer follow-up. PC(35)sGaw of 26 subjects before treatment was 0.08 g/L, which became 1.40 g/L at the third months, and then maintained at a very low level (2.64 g/L) after the seventh month. NO(3)(-)/NO(2)(-) decreased from [(734 +/- 72) x 10(-3) g/L] to [(230 +/- 41) x 10(-3) g/L] by the third month (q = 6.26, P < 0.05), and [(137 +/- 27) x 10(-3) g/L] by the seventh month, which showed no significant difference with normal control (136 +/- 20) x 10(-3) g/L, q = 3.77, P > 0.05). Eosinophil counts decreased from (0.016 +/- 0.008) to (0.014 +/- 0.007) by the third month, which was not significantly different from normal control (q = 2.94, P > 0.05). In the first fifth months the concentration of NO(3)(-)/NO(2)(-) in induced sputum exhibited a significant negative correlation with PC(35)sGaw (r(1) = -0.872, r(2) = -0.653, r(3) = -0.639, r(4) = -0.656, all P < 0.05). But eosinophil counts had no correlation with PC(35)sGaw in the first three months (r(1) = 0.237, r(2) = 0.536, r(3) = 0.675, all P > 0.05)., Conclusion: The parameters related to airway inflammation including PC(35)sGaw and sputum NO(3)(-)/NO(2)(-) may be useful in assessing asthma severity, evaluating the efficacy of treatment and adjusting medication regimens.

Background: Bisphosphonates have been used to treat many bone diseases in clinic. Bisphosphonates have also been proven useful in the management of bone metastasis in patients with breast and prostate carcinoma as demonstrated in a number of trials in vitro and in vivo, but, it is little known that the effect of bisphosphonates on lung cancer, one of the most common bone metastatic malignant tumors. This study is to investigate the effect of several bisphosphonates on inhibiting proliferation of different lung cancer cell lines in vitro, and to validate whether this inhibitive effect is comprehensive or selective., Methods: The cytotoxic effect of bisphosphonates on lung cancer cells and human normal liver cells was determined by sulforhodamine B (SRB) assay., Results: After incubation of lung cancer cells with bisphosphonates for 72h, the proliferation was inhibited in different degrees. The inhibiting activity of medronate (MDP) was the lowest, while the activity of ibandronate and incadronate (YM175) was between MDP and alendronate. The effects of bisphosphonates on human normal liver cells were different. The toxicity of MDP, ibandronate and YM175 was low, while alendronate had high toxicity. The sensitivity of lung cancer cells to bisphosphonates was also different. The sensitivity of H446 and SPC-A1 was comparatively lower, while H460 and A549 were more sensitive., Conclusions: Bisphosphonates can inhibit the proliferation of lung cancer cells and human normal liver cells in different degrees. The inhibiting effect is associated with the kind and concentration of bisphosphonates, and also the kind of lung cancer cells.

Objective: To evaluate the therapeutic efficacy of Yinxing Damo (YXDM) combined with Betahistine Hydrochloride Injection (BHI) on vertebra basilar artery ischemic vertigo (VBIV)., Methods: Ninety patients with VBIV were randomly divided into two groups; 45 patients (the treated group) were treated with YXDM and BHI intravenous dripping, once a day for 14 days. Another 45 patients (control group) were treated with Xueshuantong and BHI intravenous dripping, once daily for 14 days. The clinical syndromes and the index of the transcranial Doppler (TCD) and hemorheology were observed., Results: The total effective rate was 100% in the treated group, which was better than that in the control group 90.5%, (P < 0.05). The indexes of TCD and hemorheology in the treated group were obviously improved after treatment, (P < 0.01)., Conclusion: YXDM combined with BHT injection had better effect in treating patients with VBIV is an ideal drug for VBIV.

Objective: To study the difference between the dynamic responses of human body and human surrogate under 30 degrees supine position, and to discuss impact probability of substituting human body with human surrogate in impact tests., Method: Five volunteers experienced half-sine impact pulses, averaged 4.76, 8.96, 11.33 G, lasting for 40-60 ms on an impact tower. The human surrogate was exposed to half-sine impact pulses, averaged 6.77, 10.39, 16.93, 21.11, 24.98, 31.11 G, lasting for 40-60 ms, two times for each G level. ECG changes of the volunteers were continuously monitored before, during and after each impact., Result: Output responses at forehead and chest of human body and human surrogate increased with input increments. But there was obvious difference of the dynamic responses between human body and the surrogate to impact of low G levels. Heart rate of each volunteer had temporary increase during the process of impact, and returned to normal level soon after the impact., Conclusion: There is difference in a certain extend between the dynamic responses of human body and human surrogate. The ECG changes are induced mainly by mental stress during process of impact.

Objective: To study the chemical constituents from the herb of Gentianopsis paludosa., Method: Column chromatogrophy and spectral analysis were used to isolate and identify the constituents., Result: Six compounds were isolated and identified as 1,7-dihydroxy-3,8-dimethoxyxanthone (I), 1-hydroxy-3, 7, 8-trimethoxyxanthone (II), 1, 8-dihydroxy-3, 7-dimethoxyxanthone (III), 1-hydroxy-3, 7-dimethoxyxanthone (IV), beta-sitosterol (V), daucosterol (VI)., Conclusion: Compounds III-VI were isolated from the plant for the first time.