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Objective: To discuss the effect of cold exposure on nociception in the rats and its regulatory mechanism on transient receptor potential (TRP) ion channels in the sensory neurons, and to provide the basis for clarifying the biological mechanism of cold-sensitive pain. Methods: Sixteen female SD rats were divided into control group (n=8) and cold group (n=8). The rats in control group were exposed to the environment of (24±2) ℃, and the rats in cold group were exposed to low temperature (4 ℃±1 ℃) in an artificial intelligence climate chamber for 4 h daily, for one week. Von Frey filaments were used to detect the mechanical withdrawal threshold (MWT) of the rats in two groups； immunofluorescence staining was used to observe the expression levels of TRPA1, TRPM8, TRPV1, and TRPV4 in dorsal root ganglion (DRG) tissue of the rats in two groups, the expression levels of calcitonin gene-related peptide (CGRP) and substance P (SP) in DRG tissue of the rats in two groups, and the expression levels of TRPA1, TRPM8, TRPV1, and TRPV4 in synovial tissue of the rats in two groups. Results: Compared with control group, the MWT of the rats in cold group was significantly decreased (P< 0. 05), the expression levels of TRPA1 and TRPM8 in DRG tissue were significantly increased (P< 0. 05), the expression level of TRPV1 was significantly decreased (P<0. 05), there was no significant difference in the expression level of TRPV4 (P>0. 05), and the expression levels of CGRP and SP were significantly increased (P<0. 05). Compared with control group, the expression level of TRPA1 in synovial tissue of the rats in cold group was significantly increased (P<0. 05), while the expression levels of TRPM8, TRPV1, and TRPV4 were significantly decreased (P<0. 05). Conclusion: Short-term cold exposure can induce the hyperalgesia of the rats, and its mechanism may be associated with the changes in the expression of TRP ion channels in DRG and synovial tissues. TRPA1 sensory neurons play an important role in local joint cold pain. [ABSTRACT FROM AUTHOR]

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Objective To investigate the effect of TRPC5 on intermittent hypoxia-induced myocardial pyroptosis. Methods TRPC5 knock out (TRPC5-/-) and SD rats were each randomly divided into chronic intermittent hypoxia group (CIH group) and normoxia group (Control group) (n = 6 for each group). The myocardial fibrosis in rats was visualized by Masson staining, the levels of serum inflammatory factors were measured by ELISA and the relative expression levels of TRPC 5 and pyroptosis-related proteins were determined by Western blotting. Results Masson staining showed that the collagen volume fraction in the TRPC5-/--CIH group was higher than that in the TRPC5-/--Control group and lower than that in the WT-CIH group. The ELISA results showed that the serum levels of IL-1, IL-6, and TGF- β in the TRPC5-/--CIH group were higher than those in the TRPC5-/--Control group and lower in the WT-CIH group. Western blotting results showed that the relative expression levels of pyroptosis related proteins caspase-1, NLRP3, GSDMD, and GSDMD-N in the TRPC5-/--CIH group were higher than those in the TRPC5-/--Control group and lower than those in the WT-CIH group. Conclusion TRPC5 deficiency alleviates myocardial pyroptosis and myocardial fibrosis induced by hypoxia. [ABSTRACT FROM AUTHOR]

AIM: To examine the effects of borneol on inflammation and myocardial remodeling after myocardial infarction (MI) in mice, and to investigate the underlying mechanisms. METHODS: Eight-week-old wild-type (WT) C57BL/6 mice and transient receptor potential cation channel subfamily M member 8 (TRPM8) gene knockout ( TRPM8-/-) mice were randomly divided into sham and MI groups, and were subsequently treated with normal saline (control group) or borneol (borneol group) via gavage. Survival curves were plotted for WT and TRPM8mice with MI treated with or without borneol. After 28 d, cardiac function of the mice was assessed through echocardiography, and haemodynamic indexes were evaluated using a multi-channel physiological instrument. Infarct size, myocardial hypertrophy and interstitial fibrosis were assessed via pathological staining. In addition, inflammatory response in the peri-infarct region was detected. RESULTS: The TRPM8 expression was up-regulated in the peri-infarct region of the mice with MI (P<0. 05), and borneol had no effect on TRPM8 expression (P>0. 05). Borneol increased the survival rate, reduced the infarct size, inhibited cardiac remodeling and improved cardiac function in WT mice with MI (P<0. 05 or P<0. 01). However, it did not affect the survival rate, infarct size, myocardial hypertrophy, myocardial fibrosis or cardiac function in TRPM8mice (P>0. 05). Furthermore, borneol reduced inflammatory cell infiltration and the expression of inflammatory cytokines, tumor necrosis factor-α (TNF-α ), interleukin-1β (IL-1β), IL-6 and monocyte chemotactic protein-1 (MCP-1), in WT mice (P<0. 05 or P<0. 01) but not in TRPM8-/- mice (P>0. 05). CONCLUSION: Borneol attenuates inflammation, inhibits cardiac remodeling and improves cardiac function in mice with MI via TRPM8. [ABSTRACT FROM AUTHOR]

BACKGROUND: Changes in skeletal muscle mass have been indicated in studies addressing the effects of low-frequency pulsed magnetic fields on the structure and morphology of the skeletal muscle, but no relevant studies have been conducted on the morphologic changes that occur after chronic exposure to the lowfrequency pulsed magnetic field. OBJECTIVE: To observe the effects of chronic exposure to low-frequency pulsed magnetic fields on the maximal voluntary contraction and morphologic indicators of the quadriceps muscle of the leg, thereby providing a reference of muscle morphologic changes for the use of this technique as a strategy for muscle function improvement. METHODS: Seventy healthy subjects were recruited and randomly divided into a test group that received magnetic field stimulation and a control group that underwent sham treatment, with 35 subjects in each group, and the total duration of the trial was 4 weeks. The test group underwent low-frequency pulsed magnetic stimulation for 15 minutes every 48 hours, while the control group underwent sham treatment, with the same intervention interval and duration as the test group. After 4 weeks of intervention, changes in the maximum voluntary contraction value of the quadriceps muscle in different groups were observed, and B-mode ultrasonography was utilized as a means of assessment to observe changes in muscle thickness, muscle cross-sectional area, and pinnation angle indexes. RESULTS AND CONCLUSION: After 4 weeks of chronic exposure to low-frequency pulsed magnetic fields, 68 subjects completed the test. The maximum voluntary contraction value of the quadriceps muscle in the test group increased significantly (P=0.000), and the increment was significantly higher than that of the control group (P=0.008). Three indexes related to muscle morphology in the test group were significantly higher than the pre-test values (P=0.000), while in the control group, muscle thickness showed a significant reduction (P=0.020), there was no significant change in the pinnation angle, but a significant increase in the cross-sectional area (P=0.000). Intergroup comparisons revealed that the three indicators related to muscle morphology, including muscle thickness (P=0.012), pinnation angle (P=0.003), and cross-sectional area (P=0.049), were significantly higher in the test group than in the control group. The above data confirmed that the maximum voluntary contraction of the quadriceps muscle was significantly increased in healthy adults after 4 weeks of chronic exposure to the low-frequency pulsed magnetic field, and significant increases in the three muscle morphometric indices of muscle thickness, cross-sectional area, and pinnation angle were observed in the test group, providing a basis of muscle tissue morphology for the use of this technique as an exercise alternative and medical treatment strategy for muscle improvement. [ABSTRACT FROM AUTHOR]

BACKGROUND: Exposure to transient low-frequency pulsed magnetic field can induce and activate classical transient receptor potential channel 1, and enhance the maximal voluntary contraction force and strength endurance of local muscles, such as biceps brachii. OBJECTIVE: To use a specific low-frequency pulsed magnetic field that can activate classical transient receptor potential channel 1 as a means of improving muscle strength and to observe the attenuation process of the maximal voluntary contraction force and strength endurance of human biceps brachii after short-term stimulation. METHODS: A total of 27 normal adult healthy subjects were selected and randomly divided into training group, exposure group, and training+exposure group. The training+exposure group received resistance training immediately after 10-minute exposure to low-frequency pulsed magnetic field; the exposure group was only exposed to low-frequency pulsed magnetic field for 10 minutes; and the training group only received resistance training. The trial duration was 8 weeks. At 1-12 days of the trial, short-term muscle strength enhancement scheme was performed and post-test muscle strength was measured. The attenuation process of maximum voluntary contraction force and strength endurance was then observed in the following 6 weeks. RESULTS AND CONCLUSION: (1) The maximum voluntary contraction force value of all subjects changed significantly over time (P < 0.01), with obvious timedependent interaction effect but no interaction effect between groups, and there was no significant difference between time- and grouping-dependent interaction effects. (2) In the 1st, 2nd, 3rd, and 4th weeks, the maximum voluntary contraction force values for strength attenuation in the exposure group were significantly higher than the initial value. In the 1st and 4th weeks, the maximum voluntary contraction force values for strength attenuation in the training group were significantly higher than the initial value. In the 5th and 6th weeks, the maximum voluntary contraction force values for strength attenuation in the exposure group and training group were significantly lower than the post-test value. In the 1st, 5th, and 6th weeks, the maximum voluntary contraction force values for strength attenuation in the training+exposure group were significantly lower than the post-test value. (3) The correlation analysis of the maximal voluntary contraction force curves in the three groups indicated that the overall changes in the maximal voluntary contraction force in the exposure group were highly positively correlated with the variation trend of maximum voluntary contraction force in the training group and training+exposure group. Their variation trend was highly consistent. Compared with the exposure group, the training group showed stronger positive correlation with the training+exposure group in terms of the maximum voluntary contraction force. (4) The median frequency value of the subjects in each group changed significantly with time (P < 0.01), and there was significant time-dependent interaction effect. Time- and grouping-dependent interactions led to significant changes in the median frequency values (P < 0.01). (5) In the 2nd week, the median frequency values of muscle strength attenuation in the exposure group and training+exposure group was significantly higher than the initial value. However, compared with the post-test value, the median frequency values of muscle strength attenuation showed no significant changes in the exposure group. In the 1st and 4nd weeks, the median frequency values of muscle strength attenuation in the training group were significantly lower than the post-test value. In the 1st and 2th weeks, the median frequency values of muscle strength attenuation in the training+exposure group were significantly lower than the post-test value. (6) The correlation analysis of the median frequency change curves in the three groups indicated that the training+exposure group showed a lower positive correlation with the training group and exposure group. However, compared with the training group, the exposure group had a higher correlation with the training+exposure group. (7) After exposure to the short-term pulsed magnetic field with the intensity of 1.5 mT and the frequency of 3 300 Hz, the maximum voluntary contraction force value of muscle strength attenuates to the initial value within 6 weeks, consistent with the attenuation period of resistance training and attenuation speed after enhancement of muscle strength. During the attenuation process, there is less fluctuation in the two resistance training groups, without fatigue accumulation, in which muscle strength can be better maintained. Improved strength endurance can be maintained for at least 6 weeks. Compared with the resistance training, low-frequency pulsed magnetic field stimulation can avoid fatigue accumulation, and the anti-fatigue ability continues to increase and maintain at a better level. Resistance training combined with pulsed magnetic field has a certain benefit for endurance level. To reduce the fluctuation during the attenuation process is beneficial to the maintenance of the endurance level. [ABSTRACT FROM AUTHOR]

Objective: To investigate the correlation between serum neutrophil lymphocyte ratio (NLR), growth differentiation factor-15 (GDF-15) and transient receptor potential channel 1 (TRPC1) in elderly patients with heart failure. Methods: 100 elderly patients with chronic heart failure admitted to our hospital from January 2020 to October 2022 were selected as the research objects, and they were divided into heart failure group. In addition, 101 healthy elderly people who came to our hospital for physical examination at the same time were selected as the control group, and 98 elderly patients with arrhythmia were selected as the arrhythmia group. The serum NLR, GDF-15 and TRPC1 levels of the three groups were compared, and the serum NLR Diagnostic value of GDF-15 and TRPC1 in elderly patients with chronic heart failure. 100 patients with mental failure were evaluated according to NYHA cardiac function grading standard, including 23 patients with grade II, 38 patients with grade III, and 39 patients with grade IV. The expression levels of serum NLR, GDF-15, and TRPC1 in patients with different concentric function grading were compared. The correlation between serum NLR, GDF-15, and TRPC1 and heart function in elderly patients with heart failure was analyzed by Spearman correlation analysis. Results: The levels of serum NLR, GDF-15 and TRPC1 in the three groups were higher than those in the arrhythmia group and the control group (P<0. 05); By drawing ROC curve and analyzing the indicators with differences between groups in Table 1, the diagnostic efficacy of ROC in elderly heart failure was determined. The results showed that the area under the curve (AUC) was NLR (0.688), GDF-15 (0.667), TRPC1 (0.656), and the combination of the three (0.671). The diagnostic sensitivity of NLR was 67.61 %, the specificity was 66.85 %, the diagnostic sensitivity of GDF-15 was 60.03 %, the specificity was 67.53 %, the diagnostic sensitivity of TRPC1 was 61.24%, the specificity was 66.53 %, the combined diagnostic sensitivity of the three was 74.58 %, the specificity was 86.32 %; The levels of serum NLR, GDF-15 and TRPC1 in patients with heart failure at different levels were different, and the level of IV cardiac function was significantly higher than that of III, II(P<0.05); Spearman correlation analysis showed that the levels of NLR, GDF-15 and TRPC1 were positively correlated with cardiac function in elderly patients with heart failure (P<0.05). Conclusion: The combination of serum NLR, GDF-15 and TRPC1 has a high sensitivity and specificity for the diagnosis of senile heart failure, and has a significant correlation with the cardiac function of patients. The clinical application of NLR, GDF-15 and TRPC1 can be considered as supplementary diagnostic means of echocardiography, providing reference for clinical diagnosis. [ABSTRACT FROM AUTHOR]

To investigate the correlation between the transient receptor potential cation channel subfamily M member 3 (TRPM3) gene and duck egg laying trait, in this study, the TRPM3 gene of 90 Jingding ducks were sequenced by Illumina highthroughput sequencing to analyze the genotype frequencies of 6 SNP loci in TRPM3 and association of egg laying number and egg weight among different genotype ducks. The correlation between the mutation sites after forming haplotypes and the difference of egg laying number and egg weight was analyzed. The expression difference of TRPM3 mRNA in duck ovaries and oviducts with high and low egg laying number was detected by fluorescence quantitative PCR. The results showed that the mutation of SNPs of Z-36500134 T > C, Z-36503668 A > C, Z-36534782 G >A, Z-36684262C> T, Z-36710928 A > G, and Z-367324876 G > A of TRPM3 gene significantly reduced egg laying number and increased egg weight. There were four haplotypes of TRPM3 gene. The TAGCAG haplotype was the dominant haplotype, which was extremely positively correlated with egg laying number and extremely negatively correlated with egg weight. There was no significant difference in the expression of TRPM3 in the oviducts of the groups with high egg laying number and low egg laying number, and the expression of TRPM3 mRNA in the ovary in low egg laying number group was significantly lower than that in high egg laying number group. The results suggested that TRPM3 may play an important role in the egg laying performance of ducks, and affect the egg laying performance by affecting the ovarian function. [ABSTRACT FROM AUTHOR]

BACKGROUND: Strength quality is an essential element for human physical activity. Short-term low-frequency pulsed electrical magnetic field (PEMF) stimulation can activate classical transient receptor potential channel 1 (TRPC1) and trigger skeletal muscle growth and remodeling in mice, thereby producing a series of physiological support effects on muscle tissue. However, there is no report on whether this mechanism will alter the physiological structure and working ability of human skeletal muscle and act as a new way to improve muscle strength. OBJECTIVE: To select a specific low-frequency PEMF that can activate TRPC1 as an exogenous stimulus to observe and verify the effect of short-term stimulation on the maximum voluntary contraction force and strength endurance of human biceps brachii. METHODS: A total of 27 normal adult healthy subjects were selected and randomly divided into exercise group, PEMF group, and exercise+PEMF group, with 9 cases in each group. The training group was subjected to resistance training. The exercise+PEMF group received 10-min low-frequency PEMF stimulation (intensity 1.5 mT, frequency 3300 Hz) immediately after resistance training. The irradiation group only received 10-minute low-frequency PEMF stimulation. Training or irradiation was performed every 48 hours for 9 days. Electromyography information on the maximum voluntary contraction force was collected before and after five training sessions in the exercise group and the exercise+PEMF group, to observe whether the combination of low-frequency PEMF and resistance training will produce a gain effect. In the PEMF group, the maximum voluntary contraction force was tested only at the 1st, 3rd, and 5th sessions to track the changes in muscle strength. The maximum voluntary contraction force, one-repetition maximum force, endurance duration and median frequency were observed in each group. RESULTS AND CONCLUSION: (1) During the trial, there were significant interaction effects between the changes in maximum voluntary contraction force and time in all the subjects (P < 0.01) as well as in each group as time goes by (P < 0.05). However, there was no interaction effect between groups. (2) There was a significant improvement in the maximum voluntary contraction force, one-repetition maximum force, endurance duration, and median frequency in each group after completion of the trial. These indicators were successively improved by 19%, 23%, 28%, and 18% in the exercise group, 11%, 10%, 53%, and 18% in the exercise+PEMF group, and 28%, 18%, 27%, and 6% in the PEMF group. (3) The median frequency of the exercise+PEMF group was significantly higher than that of the PEMF group (P < 0.05), but there was no significant difference between the exercise+PEMF group and exercise group. (4) Compared with the exercise+PEMF group, the exercise group showed a significant decrease in the root mean square of maximum voluntary contraction after the first two exercise sessions (P < 0.05). (5) All these findings indicate that the short-term PEMF stimulation with intensity of 1.5 mT and frequency of 3 300 Hz can significantly improve the maximum voluntary contraction force and strength endurance of human biceps brachii. That is, low-frequency PEMF can induces TRPC1 to promote the working ability of muscle tissue, which has been effectively verified in human body. Low-frequency PEMF stimulation shares similar effects with conventional resistance training in terms of enhancing the maximum strength. Resistance training combined with PEMF stimulation shows better anti-fatigue ability and stabilizes the increase of muscle strength. This combination can stimulate more motor units of local muscle groups under the same load in the early stage of training and improve the overall training efficiency. In terms of strength endurance improvement, low-frequency PEMF stimulation can prolong isometric muscle contraction time and improve anti-fatigue ability. Compared with simple low-frequency PEMF stimulation, low-frequency PEMF stimulation combined with resistance training is more effective to develop strength endurance. [ABSTRACT FROM AUTHOR]

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Copyright of China Surfactant Detergent & Cosmetics (1001-1803) is the property of China Surfactant Detergent & Cosmetics Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Meniscus degeneration is one of the manifestations of knee osteoarthritis, but its specific molecular mechanism is still not very clear. OBJECTIVE: To explore the possible molecular biological mechanism of meniscus degeneration in knee osteoarthritis. METHODS: A meniscus degeneration model was prepared in Wuzhishan pigs through resection of the anterior cruciate ligament. Gene chip technology was used to detect differentially expressed genes in degenerated meniscus tissue. Gene oncology analysis, pathway analysis, and core gene network analysis were performed to discover relevant regulatory networks. RESULTS AND CONCLUSION: A total of 893 differentially expressed genes were detected in the study, of which cyp2c33, gcnt7, ncdn, exd3, MUC13, ppp1r3d, nphp3, upb1, CD81 and prph were differentially expressed most significantly. The regulation of nitric oxide biosynthetic process in gene oncology analysis, TRP channel in pathway analysis, and MDFI gene in core gene network analysis may play key roles to induce meniscus degeneration. In short, we have established a reliable animal model of meniscus degeneration, and more completely revealed the differentially expressed genes, biological processes and signal pathways related to meniscus degeneration after knee osteoarthritis. These will provide molecular targets for the diagnosis and treatment of the disease in the future. [ABSTRACT FROM AUTHOR]

[Objectives] This study aimed to investigate the effect of phenylalanine(Phe) on cholecystokinin(CCK) secretion in swine duodenal perfusion in vitro. In addition,the potential roles of the calcium-sensing receptor(CaSR),umami receptor(type 1 taste receptor 1/3,T1R1/T1R3) and downstream phospholipase C(PLC) and transient receptor potential subfamily M member 5(TRPM5) were also demonstrated. [Methods] Western blot was used to detect whether CaSR expressed in swine duodenum. Based on the above result,the effect of Phe on CCK secretion was explored by using in vitro perfusion system and the roles of the CaSR,T1R1/T1R3 and downstream PLC,TRPM5 on CCK secretion were investigated by the addition of inhibitors or agonists. [Results] CaSR protein was expressed in pig duodenum,and 50 mmol·L-1 L-Phe instead of D-Phe stimulated CCK secretion(P<0.05). CaSR inhibitors NPS 2143(25 μmol·L-1) or calhex 231(20 μmol·L-1) suppressed CCK secretion(P<0.05),however,T1R1/T1R3 agonist inosine monophosphate(IMP,2.5 mmol·L-1) or inhibitor lactisole(5 mmol·L-1) had no effect on CCK secretion(P>0.05). The addition of PLC antagonist U-73122(10 μmol·L-1) or TRPM5 inhibitor triphenylphophine oxide(TPPO,100 μmol·L-1) inhibited CCK secretion(P<0.05). [Conclusions] CaSR and downstream PLC/TRPM5 played an important role in L-Phe induced CCK secretion from swine duodenum in vitro. [ABSTRACT FROM AUTHOR]

Objective: To explore the diagnostic value of combined detection of serum cancer antigen 19-9 (CA19-9), carbohydrate antigen 125 (CA125), multi-slice spiral CT and nuclear magnetic resonance (MRI) in cholangiocarcinoma, and to analyse the correlation between tumor markers and invasive molecules. Methods: 62 patients with cholangiocarcinoma who were admitted to affiliated Hospital of Chifeng University from January 2017 to August 2018 were selected as cholangiocarcinoma group. Another 55 patients with benign bile duct lesions admitted to our hospital during the same period were selected as the benign bile duct lesion group. The levels of serum CA19-9, CA125 and tissue invasive molecules content were compared between the two groups. The manifestations of multi-slice spiral CT and MRI in patients with cholangiocarcinoma and benign lesions of bile duct were observed. The diagnostic value of serum CA19-9, CA125, multi-slice spiral CT and MRI in cholangiocarcinoma were analyzed. The correlation between serum CA19-9, CA125 levels and tissue invasive molecules content were analyzed. Results: The serum levels of CA19-9 and CA125 in cholangiocarcinoma group were higher than those in benign lesions of common bile duct group. The contents of lysinoyl oxidase-like protein-2 (LOXL2) and transient receptor potential cation channel 7 (TRPM7) in cholangiocarcinoma group were higher than those in benign lesions of common bile duct group. The content of E-cadherin in tissues was lower than that in benign lesions of common bile duct group (P<0.05). Multi-slice spiral CT imaging signs: cholangiocarcinoma can be seen in the common bile duct, hepatic duct round or quasi-round high-density shadow with wall infiltration, irregular nodules in the bile duct, blurred boundaries between the mass and surrounding tissues, cystic duct and neck infiltration of the gallbladder, liver lobe atrophy, lymph node enlargement, etc. Benign bile duct lesions were mostly round or quasi-circular high-density shadow, wall infiltration and lymph node enlargement were rare. MR imaging signs: cholangiocarcinoma intrahepatic bile duct and liver tissue demarcation was not clear, the mass was irregular or lobulated, gallbladder enlargement, intrahepatic and extrahepatic bile duct dilatation in varying degrees, pancreatic duct dilatation, liver lobe atrophy, lymph node enlargement. Benign bile duct lesions were usually "cup-shaped" low-signal filling defect, and "bird's mouth" changed appear above bile duct obstruction. The sensitivity, specificity and accuracy of combined detection of serum CA19-9, CA125, multi-slice spiral CT and MRI in the diagnosis of cholangiocarcinoma were higher than those of CA19-9, CA125, multi-slice spiral CT and MRI alone. The serum levels of CA19-9 and CA125 in patients with cholangiocarcinoma were positively correlated with tissue LOXL2 and TRPM7 content, but negatively correlated with tissue E-cadherin content (P<0.05). Conclusion: The combined detection of serum CA19-9, CA125, multi-slice spiral CT and MRI has a good value in the diagnosis of cholangiocarcinoma. The levels of serum CA19-9 and CA125 in patients are correlated with tissue invasive molecules, which can provide a basis for the evaluation of malignant degree of cholangiocarcinoma. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The Transient receptor potential (TRP) channel is a non selective cation channel, and is widely distributed in neuronal and non neuronal cells. As a subtype of TRP channel, TRPA1 is a temperature sensitive TRP channel which temperature activation threshold is related to species. TRPA1 in mouse is activated by cold temperature (<17 °C), however TRPA1 in the green lizard is sensitive to warmtemperature (35.8 °C). In addition to the response to the temperature, TRPA1 can also be activated by chemical stimuli, mechanical stimulation and other physical and chemical means. A recent study shows that a gain-of-function mutation in TRPA1 can lead to familial paroxysmal pain syndrome. Due to TRPA1 has been proposed to function in many physiological and pathological processes such as temperature perception, pain producing, asthma and itching, TRPA1 has become one of the most intensively studied ion channels and attracted wide attention. In this review, we summarize the biophysical characteristics of TRPA1 channels, and discuss recent advances in our understanding of TRPA1 physiology, pharmacology, and molecular function in order to provide theoretical basis for clinical treatment of related diseases. [ABSTRACT FROM AUTHOR]

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Transient receptor potential (TRP) channels superfamily of ion channels were first discovered in a mutant of Drosophila melanogaster which were visual defected, it was named by the transient electrophysiologieal signals when we used the light to stimuli this mutant. Thirteen genes were identified in Drosophila based on the sequence homology, and it was divided into seven subfamilies, TRPC ,TRPA ,TRPM ,TRPV,TRPN, TRPP and TRPML. TRP channels also found in other animals, yeast, alage and so on. TRP superfamily involved in all physiological and sensory modalities in insects, such as vision, olfaction, hearing, thermosensation, mecha-nosensation, etc. Latest research shows that the protein complex of nanchung and inactive which belongs to TRPV subfamily of insect was the target of pymetrozine. In this paper, the nomenclature, classification, structure, evolution and activation mechanism of TRP channel family are introduced respectively, the TRP channels functions of physiological and behavioral in insects are reviewed in detail based on the latest literature. [ABSTRACT FROM AUTHOR]

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PURPOSE: To investigate the expression of TRPVI and IL-1α in Sprague-Dawley rats'periodontal ligament (PLD) during experimental tooth movement of different orthodontic force and duration, and explore the role of TRPV1 and IL-1α in orthodontic pain. METHODS: 66 Sprague-Dawley rats were divided into control group (n=6),sham operation group (n=6) and experimental group (n=54) randomly. Orthodontic force was applied on right first maxillary molar in rats and the changes of TRPV1 and IL-α expression were detected by real-time PCR at 4 h, 8 h, 1 d (three subgroups were added according to different forces: 1 d-30 g, 1 d-50 g, 1 d-80 g ), 3 d, 5 d, 7 d, 14 d after tooth movement. The data was analyzed using SPSS 13.0 software pakage. RESULTS: Following the experimental tooth movement, the expression of TRPVI and IL-1α in periodontal tissues were significantly up-regulated from 4 h to 7 d, with a peak at 1 d and returned to normal level at 1 week. The greater force applied during experimental tooth movement at 1 d, the higher expression of TRPVI and IL-1α were detected in periodontal tissues. CONCLUSIONS: Experimental tooth movement leads to regular change of TRPV1 and IL-1α expression in periodontal tissues, which indicates that TRPV1 and IL-1α may play an important role in orthodontic pain. [ABSTRACT FROM AUTHOR]