[Objective] With profound and complex changes in the human environment, different types of public health emergencies are occurring more frequently. The medical equipment industry has become one of the world's fastest-growing industries, making it particularly important to cultivate "medical--industrial integration" talents with "medical" and "engineering" expertise. Negative pressure isolation chambers, the most efficient means for preventing cross-infection, are mainly used for transporting individuals with highly contagious diseases such as COVID-19 (Corona Virus Disease 2019), SARS (Severe Acute Respiratory Syndrome), avian influenza, and Ebola. However, current negative pressure isolation chambers have limitations, such as a lack of individual manipulation and mobility, low isolation and protection levels, and poor comfort, which seriously compromise patient care efficiency. Therefore, a new type of mobile individual isolation chamber is proposed in this study to improve protection levels and comfort. [Methods] This study developed a simulation analog test system for a mobile individual isolation chamber comprising an aerosol generation chamber, the isolation chamber body, and a remotely operated containment chamber. This system simulated the patient's breathing process and aerosol-contaminated environments, moving at a speed of 10 km/h to mimic patient transfer. The chamber's clinic window was pierced to simulate routine testing and diagnostic activities such as nucleic acid sampling, blood draws, and injections. NaCl aerosol, with a logarithmic particle size distribution in the range of 15-205 nm, was used as the test medium to simulate viral aerosols. Key performance indexes of the innovative mobile individual isolation chamber, such as leakage, permeation, percentage of O2 and CO2, temperature, and relative humidity were tested under different movement states, negative pressure levels, and clinic window treatments. [Results] The chamber could be stabilized to the preset negative pressure value within 1-10 min of operation. Leakage rates for the chamber when at rest and moving were 0.000 3%-0.001 8% and 0.038 8%-0.637 4%, respectively. Permeation rates before and after clinic window piercing were maintained at 0.436 1%-2.565 8% and 0.599 5%-3.56 58%, respectively. Increased negative pressure significantly reduced leakage but significantly increased permeation. O2 and CO2 levels inside the chamber could be maintained at 20%-22% and 0.03%-0.04%, respectively. Under external conditions of 32 °C and 35% relative humidity (RH), the chamber's internal temperature and RH were adjusted to 20 °C-24 °C and 40%-48% within 5 min. The chamber temperature and humidity regulation rate accelerated with increasing negative pressure, and window piercing did not affect O2 and CO2, temperature, and RH levels inside the chamber. [Conclusions] Results show that the new mobile individual isolation chamber offers superior protection and good comfort. This study promotes students' understanding of personal protective equipment, stimulates their creativity and practical ability, provides a reference for practical applications of mobile individual isolation chambers, and supports the development of "medical-industrial integration" talents. [ABSTRACT FROM AUTHOR]