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Temporomandibular joint osteoarthritis (TMJOA) is characterized by progressive cartilage degeneration, subchondral bone remodeling, chronic inflammation in the synovial tissue and osteophyte formation. Chondrocyte apoptosis is one of the main factors of cartilage degeneration. Prevention and treatment of TMJOA can be achieved by inhibiting premature or excessive chondrocyte apoptosis. Primary cilia protruding from the surface of chondrocytes can sense mechanical stimulation and induce chondrocyte differentiation. Studying the role of primary cilia in TMJOA will help us understand the pathogenesis of TMJOA and construct a reasonable and effective treatment strategy.

[Objective] To improve the detection accuracy of early apple spoilage zone. [Methods] An apple spoilage detection method was proposed based on generative adversarial network and convolutional neural network. The Pix2PixHD model was used to generate near-infrared imaging data of stored apples in the early postharvest metamorphic area. The Mask R-CNN model was used to segment the generated near Infrared image to detect the deterioration zone in the apple. Based on generative adversarial network and convolutional neural network technology, the early deterioration region segmentation and prediction of postharvest apples were implemented by using the generated near-infrared imaging data on a low-cost embedded system with artificial intelligence function. [Results] The average accuracy of this method was 1.825%～10.435% higher than that of the other nine methods. The Pix2PixHD generated a visible NIR image from an RGB image at 17 frames per second, and the Mask R-CNN was able to segment spoilage areas in an apple image at 4.2 frames per second. [Conclusion] The proposed method is expected to facilitate the development of low-cost food quality controllers.

This paper briefly describes the theoretical basis of the DRY-1B capacitive component drilling strain gauge (drilling strain gauge). It discusses critical technologies such as micro-displacement sensing, noise reduction, temperature control, performance testing, and calibration. The strain gauge achieved high resolution (≥5×10−11ε), wideband (10–100 Hz optional), extensive dynamic range (≥1×10−3ε), 24-bit AD recording, low power consumption (< 3W), and other technical indicators. Its performance is better than the United States PBO and Japan borehole strain gauge of the same period, and it is an international leading long-term observation instrument for crustal movement, which can basically meet the observation requirements of creep movement with slow accumulation of long-term strain and seismic and volcanic activity with a rapid change of short-term strain. Since 2008, through the application of more than 20 geostress stations, the borehole strain gauge has recorded a large amount of strain information, such as crustal deformation, fault activity, co-seismic strain wave, strain step, and ore pressure activity. Based on the results of the self-consistency test of strain monitoring data of the geostress station in the Beichangshan Mountain and the analysis of seismic mapping capacity of the Turkey earthquake, it is found that the strain curves of the 1#+3# and 2#+4# capacitance sensors at the Beichangshan Mountain stress station are generally stable, and the correlation coefficient R2 is 0.95. The annual variation rate of the differential strain of 1#–3# and 2#–4# elements is 10−8 magnitude, which reflects that the shear stress is dominant in the Long Island area and the stress environment of seismic activity is relatively high. The strain gauge was used to observe the apparent co-seismic strain response of Turkey’s M 7.8 and M 7.5 earthquakes on February 6, 2023. In particular, it obtained the M 7.8 main seismic surface wave period of 50–60 s, presenting an out-facing wave anomaly. Theoretically, the strain wave generated by the M 0.74 earthquakes within 100 km can be distinguished, and the application demonstration effect has been achieved. The borehole strain gauge has good popularization value and application prospects in geodynamics research and internal dynamic geological disaster monitoring.

We carried out hydraulic fracturing in-situ stress measurements in an 1881-m deep borehole at the Shuiwangzhuang gold deposit and obtained the variation law of deep in-situ stress with depth in the mining area. The measurement results show that the maximum principal stress has an increasing linear trend with depth. The horizontal stress dominates the in-situ stress state within 800 m, and the vertical stress gradually transitions to the maximum principal stress with increasing depth. The maximum horizontal principal stress ranges from 11.22 to 45.69 MPa, the minimum horizontal principal stress from 7.28 to 36.17 MPa, and the vertical principal stress from 8.44 to 48.27 MPa; The direction of the maximum horizontal principal stress is NWW-trending. We analyzed the characteristics of the deep orebody’s in-situ stress according to the stress value and the direction of the maximum horizontal principal stress, which reveals that the deep in-situ stress of the Shuiwangzhuang mining area belongs to the generally low level in the Zhaoyuan–Laizhou area. We discussed the tendency of rockburst in the underground roadway during deep excavation under a high confining pressure environment based on rock mechanics parameters of drill cores, engineering rock grading standards, and elastic strain energy theory of rock bodies. The Shuiwangzhuang gold orebodies generally belong to the rockburst-free strata or strata with weak rockbursts. However, there is a strong rockburst tendency at depths such as 1102.78 m and 1379.40 m. The gold ore body is at a depth of 1680.40~1684.90 m, generally in the rockburst-free area. The above research results can provide an essential scientific basis for deep mine construction and mining design.

The Yalu River fault zone is an essential branch of the Tanlu fault system with intense present-day tectonic activity. Over 60 hot springs are exposed along the fault zone with abundant geothermal resources. In order to find out the present-day crustal stress state and fault activity in the Wulongbei area of the southern section of the fault zone and to study the control and long-term influence of the fault activity on the geothermal water bodies of hot springs, in-situ stress measurements of 12 sections were carried out in the area by hydraulic fracturing method. The results showed that the maximum and minimum horizontal principal stresses (SH and Sh) range from 6.00 to 13.52 MPa and 3.18 to 7.26 MPa, respectively, in the depth range of 36.80–215.50 meters. In general, the three principal stresses showed an increasing trend with depth; in the middle section of the fracture zone (198.60–207.80 m), the three principal stress values meet the relation of SH>Sv>Sh, which is favorable to the strike-slip activity and has some potential for water-rich hydraulic conductivity, while the three principal stress values of the upper section (36.80–196.63 m) and lower section (215.50 m) accord with SH>Sh>Sv, which is favorable to the reverse fault activity, with poor longitudinal continuity of heat flow channels and poor hydraulic conductivity. Based on the present-day relationship between stress field characteristics and fault activity, it is deduced that the decline of the hot spring level in the Wulongbei area may be due to the fault activity under compressive stress, which gradually reduced the space of the hydraulic conductivity system by extrusion, causing the increase of discharge of runoff in other directions and the reduction of hot spring water supply. Based on the Coulomb frictional instability theory, the in-situ stress value in the depth range of 36.80–113.20 m in the fault zone reaches the lower limit of the critical stress value required for its activity. There is a possibility of dislocation in the future, and the fault activity may change the hot spring geothermal water supply channel. The research results have theoretical significance in studying the role of faults in controlling hot spring geothermal water and applying in-situ stress measurements near fault zones in geothermal research.

The article reviews the development of the volumetric borehole strain gauge. In response to the current problems of insufficient stability and bandwidth and low calibration accuracy of the volumetric strain gauge, a TY-2B-type small volumetric borehole strain gauge was developed with innovative improvements in the hydraulic sensor, control circuit, and calibration method. The improved hydraulic sensor improves the accuracy and reduces the instrument’s volume; the improved control circuit increases the sampling rate, bandwidth, and the instrument’s stability; the innovative piezoelectric ceramic calibration technology raises the reliability of the monitoring data. The test results show that the improved TY-2B volumetric strain gauge has a low power consumption of less than 3 W, good long-term stability, high sensitivity with a resolution of 10-11 ε, and suitable high-frequency and low-frequency. It has a sampling rate of 100 Hz and can acquire complete seismic strain waveforms with precise and stable solid tide waveforms. It is small and light, with a reduced outer diameter of Φ89 mm for Φ100 mm drilling, a length of 1300 mm, and a weight of 45 kg for easy transport and installation. After 15 years of laboratory and field station testing, it obtained good monitoring data and demonstrated its highly sensitive seismic reflection capability. The observed response of the volumetric strain station in the northern section of Longmen Mountain to the 2010 Yushu earthquake and the 2023 Turkey earthquake shows that the TY-series high-precision volumetric strain gauge is not only a static strain gauge but also a broad-frequency strain seismograph with dynamic-static calibration capability. It has a unique advantage over pendulum seismometers in that it can observe both the long-term slow deformation and accumulation of deformation in the earth’s crust and the transient subtle features of crustal rupture and deformation. The long-term trends of the monitoring curves obtained from the Qingchuan–Hanzhong volumetric strain stations since the Wenchuan earthquake and the Guangzhou station since 2021 are consistent with the regional geological characteristics reflected by the seismic and tectonic geological data, indicating that the TY-2B volumetric strain gauge can meet the needs of geological scientific research and geological hazard observation.

In order to obtain the in-situ stress field characteristics and analyze tectonic stability in the Motuo key area of the eastern Himalayan syntaxis, the in-situ stress measurement of one in-situ stress hole and 11 test sections of the Xirang section of the Motuo fault zone were carried out by the hydraulic fracturing method. The results show that the maximum and minimum horizontal principal stress values (SH, Sh) in the test section of 61.43−121.34 m are 3.05−14.50 MPa and 2.16−9.87 MPa, respectively, and the vertical principal stress values (Sv) are 1.63−3.31 MPa, namely, SH>Sh>Sv. The in-situ stress field at the measuring point is dominated by horizontal compression, and all of them belong to the in-situ stress state of reverse fault. The principal stress values gradually increase with the increase of depth, and the dominant direction of the maximum principal stress is NEE. In the whole range of in-situ stress depth, the lateral pressure coefficients (Kav) are 1.39−4.38, the maximum horizontal stress coefficients (KHv) are greater than 1, and the ratio increases with the increase of depth. The regional stress field of this key area is dominated by horizontal stress and it is highly directional. The horizontal stress coefficients (KHh) of all test sections are 1.23−1.66, which are similar to the calculation results of in-situ stress characteristic parameters in Linzhi−Tongmai section. The horizontal tectonic stress of the shallow level at 98 m is relatively small, and the stress accumulation level is low. The friction coefficient required to maintain fault stability is smaller than the critical friction coefficient of actual fault, and the tectonic environment is relatively stable. When the depth exceeds 98 m, the friction coefficient required to maintain fault stability is close to the critical friction coefficient value of the actual fault due to the dominant role of horizontal tectonic stress, and there is a small risk of fault instability slip. The superposition of the Coulomb stress change in the sinistral strike-slip direction and the thrust direction caused by the strong regional earthquakes on the fault plane of the Motuo fault zone in the study area are all negative numbers, which inhibits fault slip and does not increase the risk of fault activity in the study area.

This paper summarized the concept and characteristics of "Internet +", analyzed the application and difficulties of "Internet +" in drug management for chronic diseases, summarized the influencing factors of drug compliance of patients with chronic diseases, and put forward suggestions and prospects for the development of Internet + in drug management for chronic diseases. (本文综述了“互联网+”的概念、特点, 分析“互联网+”在慢性病药物管理中的应用情况和难点, 概述慢性病患者服药依从性的影响因素, 并对“互联网+”在慢性病药物管理中的发展提出建议和展望。)

A new method using gradient boosting regression tree(GBRT) which is optimized by logistic regression(LR) to predict working resistance of hydraulic support is proposed, avoiding shortcomings of current determining methods. Add learning rate to GBRT to limit the learning rate of sub-models and prevent over-fitting; using LR to optimize sample parameters to establish LR-GBRT regression prediction model; the model is applied to predict resistance of hydraulic support, and the prediction result is compared with linear regression(LR), support vector model(SVM), decision tree regression(DTR), elastic net regression(EN). The results show that the model has better generalization performance and higher prediction accuracy. It can effectively predict resistance of hydraulic support .

The influence of laser cutting parameters on cutting quality of tussah by CO laser was studied, and the optimal combination of technical parameters was obtained. Orthogonal test method was used to study the influence of light intensity, feed speed and lens on seam width and seam depth, and to analyze the variation rule of main effect and interaction test data. The results showed that the main factor affecting laser cutting was lens height, and the interaction between feed speed and light intensity was significant. In actual production, the optimal combination of technical parameters can be determined according to different cutting effects required, such as laser cutting of tussah wood with water content of 6.5%, in order to obtain seam depth of about 3 000 μ m, seam width of about 200 μ m, 5 mm lens height, 40 mm/s feed speed and 40% laser intensity. [ABSTRACT FROM AUTHOR]

In the construction project of Shuidishan Reservoir in Shenshan Cooperation District, the UAV oblique photogrammetry technology is used to obtain the real 3D model of the project area, combined with manual measurement, diversified geographic information data are obtained. Based on this, BIM forward design is used to complete project formulation. The real 3D model, digital elevation model of the survey area and reservoir BIM model are used as the foundation of spatial data. The function of three-dimensional visual analysis and calculation is realized and the UAV oblique photogrammetry is successfully applied to the BIM design of the project, by serving the design and construction management of reservoir construction projects better, it provides a technical reference for the fusion application of UAV oblique photogrammetry technology and BIM Technology in reservoir construction projects. [ABSTRACT FROM AUTHOR]

The influence of surface treatment with compound silicates and silicate/silane composite system on durability of concrete with different strength was studied. SEM and FTIR were used to explore the mechanism of surface treatment. The enhancement effects were compared by anti-chloride ion, carbonization resistance and freeze-thaw resistance tests. The results show that the enhancement effects of silicate/ silane composite treatment are better than that of compound silicates alone, and the improvements effect is more obvious with decrease of concrete grade. Compared with untreated concrete,the 6 h electricity flux of C50 concrete has decreased by 10. 0%, C40 concrete by 14. 4%, and C30 concrete by 29. 4%. Mean-while,the carbonization depth has also decreased from 12. 1% of C50 to 23. 8% of C40 and 33. 0% of C30. At the same time,using silicate/silane composite for concrete surface treatment can effectively improve the number of freeze-thaw cycle,reduce the mass loss rate,and endow the concrete with better durability. [ABSTRACT FROM AUTHOR]

Aiming at the problem of permanent magnet synchronous motor(PMSM) sensorless starting at low speed, the method of open-loop speed and closed current loop was researched, which drives motor by given current vector. After the analysis of the transition from single current loop to speed closed loop control based on the startup of single current loop control, a method that d-axis current control was brought to increase " torque-power angle balance" area was presented to optimize startup and transition. The current and power angle changes of transition in the proposed method were tested by digital AC control system using DSP. The results indicate that the proposed method is feasible to start and switch, the motor can start smoothly from zero speed. The current and power angle changes of transition are decreased rapidly, and the transition from single current loop to speed closed loop is smooth. Finally, the motor can be accelerated to normal operating mode. [ABSTRACT FROM AUTHOR]

To solve the problem of low image matching accuracy, large amount of calculation and low robustness of the object on existing augmented reality system based on natural features, this paper proposed a new image matching algorithm based on affine-invariant closed region and SURF. First, the input image with histogram equalization for image enhancement to obtain a binary image and extracted the closed region in the image as the image of the affine invariant region. Second, it used SURF detection algorithm to extract the image of the closed region feature description. Finally, it finished the procedure of image matching using bidirectional matching with SURF algorithm. Experiment results indicate that the image matching accuracy largely improved while calculations consumed less time. The proposed method can meet the requirements of augmented reality systems. [ABSTRACT FROM AUTHOR]

The identification of plants of subtribe Swertiinae (Gentianaceae) holds importance in that many plants o fSwertiinae are used as material for Tibetan medicine "Dida" (Zangyinchen). In order to provide accurate identification of these plants, we evaluate the performance of 4 candidate barcodes (nuclear ITS, chloroplast rbcL.matK and trnH-psbA) in 50 individuals of 25 species of Swertiinae. The result shows that ITS not only perform best(80%)among 4 individual barcode; any barcode used in combination with ITS receive 100% species identification rate. Take the high discrimination power of matK+ITS in seed plants into consideration, we recommend matK+ITS combination as the standard barcode combination for plants of Swertiinae. [ABSTRACT FROM AUTHOR]

Landscape pattern indices are the commonly used tools for the quantitative analysis of landscape pattern. However, the traditional 2D landscape pattern indices neglect the effects of terrain on landscape, existing definite limitations in quantitatively describing the landscape patterns in mountains areas. Taking the Qixia City, a typical mountainous and hilly region in Shandong Province of East China, as a case, this paper compared the differences between 2D and 3D landscape pattern indices in quantitatively describing the landscape patterns and their dynamic changes in mountainous areas. On the basis of terrain structure analysis, a set of landscape pattern indices were selected, including area and density (class area and mean patch size), edge and shape (edge density, landscape shape index, and fractal dimension of mean patch), diversity (Shannon's diversity index and evenness index), and gathering and spread (contagion index). There existed obvious differences between the 3D class area, mean patch area, and edge density and the corresponding 2D indices, but no significant differences between the 3D landscape shape index, fractal dimension of mean patch, and Shannon's diversity index and evenness index and the corresponding 2D indices. The 3D contagion index and 2D contagion index had no difference. Because the 3D landscape pattern indices were calculated by using patch surface area and surface perimeter whereas the 2D landscape pattern indices were calculated by adopting patch projective area and projective perimeter, the 3D landscape pattern indices could be relative accurate and efficient in describing the landscape area, density and borderline, in mountainous areas. However, there were no distinct differences in describing landscape shape, diversity, and gathering and spread between the 3D and 2D landscape pattern indices. Generally, by introducing 3D landscape pattern indices to topographic pattern, the description of landscape pattern and its dynamic change would be relatively accurate. [ABSTRACT FROM AUTHOR]

This paper presents a new real-time path planning algorithm for robot in dynamic uncertainty environment, which has good applicability. The first part of the algorithm is the analysis and interpretation of the environment information by searching the characteristic information (Double-Safe-Edges, DSE), so it can catch dynamic obstacle; estimate the state of the dynamic obstacle and update the characteristic information to the second part of the algorithm at the same time. The second part is the choice of double-safe local goal to generate the motion command by using heuristic algorithm. The result of simulation proves that the DSE algorithm is real-time and has good applicability in dynamic uncertainty environment. [ABSTRACT FROM AUTHOR]

Objective To analyze changes in proteoglycan and its correlation with alveolar bone resorption in periodontitis. Methods Twelve eight-week-old C57BL/6J male mice were selected, and the periodontitis model was established by ligating the right maxillary second molar with 6-0 silk thread. The nonligated part of the left maxilla was used as the control. The mice were killed 14 days after the operation. Micro-CT was used to assess alveolar bone resorption. HE staining was used to observe the alveolar bone profile, and TRAP staining was conducted to examine the positive rate of osteoclasts. The expression of proteoglycan-related genes, such as aggrecan (ACAN), biglycan (BGN), versican (VCAN), decorin (DCN), osteoclast-related genes, such as cathepsin K (CTSK), matrix metalloprotein-9 (MMP-9), and receptor activator of nuclear factor kappa-B ligand (RANKL), and inflammation-related genes, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), was detected by real-time quantitative PCR. Additionally, the correlation of the expression of proteoglycans with osteoclast-related genes and inflammation-related genes was evaluated by Pearson correlation analysis. Results The resorption of alveolar bone on the periodontitis side increased. TRAP staining showed that the number of osteoclasts was substantially increased in the maxilla with periodontitis. Real-time quantitative PCR demonstrated that compared with the control side, the expression of proteoglycan-related genes, such as ACAN, BGN, and DCN, was decreased, whereas the expression of the VCAN gene was significantly increased in the periodontitis side. Meanwhile, the expression of osteoclast-related genes, such as CTSK, MMP-9, and RANKL, and inflammation-related genes, such as IL-1β, IL-6, and TNF-α, was markedly increased in the periodontitis side (P

Objective: To compare the trueness of incisal guidance of implant-supported single crowns designed by patient-specific motion (PSM) with that designed by average-value virtual articulator (AVA)., Methods: The study had recruited 12 participants with complete dentition and stable incisal guidance. An intraoral scanner was used to scan digital casts and record two types of patient-specific motion (data only including protrusive movement, and data including protrusive movement and lateral protrusive movement). The lingual surfaces of the maxillary incisors which guided the protrusive movement was selected and elevated to create a reference cast. A maxillary central incisor of original casts was vir-tually extracted and implanted to generate a working cast. The Dental system software program was used to design implant-supported single crowns with the anatomical coping design method. The incisal guidance was designed by different methods. The incisal guidance in control group was designed by the average-value virtual articulator. The incisal guidance in experiment groups was designed by the patient-specific motion only including protrusive movement (PSM1) and with the patient-specific motion including protrusive movement and lateral protrusive movement (PSM2). The incisal guidance of prosthesis designed by these 3 methods were compared with the original incisal guidance in Geomagic Control 2015 (3DSystem, America). The measurements included: Average of positive values, ratio of positive area and maximum value reflecting supra-occlusion; average of negative values, ratio of negative area and minimum value reflecting over-correction; and root mean square reflecting overall deviation., Results: Statistical data were collected using the median (interquartile range) method. The average of positive values, ratio of positive area and average of negative values of the PSM2 group were smaller than those of the control group [8.0 (18.8) μm vs . 37.5 (47.5) μm; 0 vs . 7.2% (38.1%); -109.0 (63.8) μm vs .-66.5 (64.5) μm], and the ratio of negative area of PSM2 group was larger than those of the control group [52.9% (47.8%) vs . 17.3% (45.3%)], with significant differences ( P all < 0.05). The ratio of positive area [0.1% (7.0%)] and average of negative values [-97.0 (61.5) μm] of PSM1 group, were smaller than those of the control group, and the ratio of negative area [40.7% (39.2%)] of the PSM1 group was larger than that of the control group, with significant differences ( P < 0.05). The average of positive values [20.0 (42.0) μm] and ratio of positive area of PSM1 group was larger than that of the PSM2 group with significant differences ( P < 0.05)., Conclusion: To establish the incisor guidance of implant-supported single crowns, compared with the average-value virtual articulator and the patient-specific motion only including protrusive movement, the patient-specific motion including protrusive movement and lateral protrusive movement is more conducive to reducing the protrusive interference of prosthesis and improving the occlusal fit.

Objective: To evaluate the efficacy and safety of allogeneic hematopoietic stem cell transplantation (allo-HSCT) with decitabine (Dec)-conditioning regimen in the treatment of myelodysplastic syndrome (MDS) and MDS transformed acute myeloid leukemia (MDS-AML)., Methods: The characteristics and efficacy data of 93 patients with MDS and MDS-AML who received allo-HSCT in our center from April 2013 to November 2021 were retrospectively analyzed. All patients were administered by myeloablative conditioning regimen containing Dec (25 mg/m 2 /d×3 d)., Results: Among the 93 patients, 63 males and 30 females, were diagnosed as MDS（ n ＝77）, MDS-AML（ n ＝16）. The incidence of I/II grade regimen-related toxicity (RRT) was 39.8%, and III grade RRT was only found in 1 patient (1%). Neutrophil engraftment was successful in 91 (97.8%) patients after a median neutrophil engraftment time of 14 (9-27) days; Successful platelet engraftment was achieved in 87 (93.5%) patients, with a median engraftment time of 18 (9-290) days. The incidence of acute graft versus host disease（aGVHD） and grade III-IV aGVHD was 44.2% and 16.2%, respectively. The incidence of chronic graft versus host disease（cGVHD） and moderate-to-severe cGVHD was 59.5% and 37.1%, respectively. Of the 93 patients, 54 (58%) developed posttransplant infections, among which lung infection (32.3%) and bloodstream infection (12.9%) were the most common. The median follow-up after transplantation was 45 (0.1-108) months. The 5-year overall survival (OS) rate, disease-free survival (DFS) rate, treatment-related mortality, and cumulative incidence of relapse were 72.7%, 68.4%, 25.1%, and 6.5%, respectively. And the 1-year graft-versus-host disease/relapse-free survival rate was 49.3%. The patients in different group of relative high-risk prognostic scoring or low-risk prognostic scoring, with or without poor-risk mutation(s), with mutations number ≥3 or <3 had similar 5-year OS rate (more than 70%). Multivariate analysis showed that the incidence of grade III-IV aGVHD was the independent risk factor affecting OS（ P =0.008）and DFS ( P =0.019)., Conclusion: Allo-HSCT with Dec-conditioning regimen is feasible and effective in the treatment of patients with MDS and MDS-AML, especially those in high prognostic risk and with poor-risk mutations.

In order to ensure the safety of animal food and regulate the application of veterinary drugs, it is necessary to strictly monitor their content, and to constantly improve the methods used to detect non-specific, illegally added substances in veterinary drugs. A study about the screening, analysis, and confirmation of illegal additives in enrofloxacin powder (used for aquaculture) using non-targeted analysis technology was introduced. First, an enrofloxacin powder test solution under acidic conditions was prepared by adding formic acid, and an enrofloxacin powder test solution under alkaline conditions was prepared by adding sodium carbonate. An ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was used to assay the test solutions for the presence of unknown additives. Results revealed two high-response unknown peaks in the acidified test solution, with retention times of 1.870 min and 5.122 min respectively. In the alkalized test solution, only one high-response unknown peak was found, with a retention time of 5.122 min. The ultraviolet spectrum characteristic peaks at 5.122 min in acidified and alkalized test solutions were similar, but the peak area in the alkalized test solution was almost ten times that in the acidified solution. Two potential unknown substances were detected. Unknown substance 1 (1.870 min) and unknown substance 2 (5.122 min) may transform under acidic or alkaline conditions. Ultra-performance liquid chromatography-time of flight high resolution mass spectrometry (UPLC-TOF-HRMS) was used to analyze the unknown compounds in more detail. The acidified and alkalized test solutions were detected in the positive and negative ion modes of mass spectrometry, respectively. Accurate mass of the precursor ion, characteristics of secondary ion fragments, and isotopic intensity ratio of the two unknown substances were collected. This information was imported into SCIEX OS software. The molecular formula of the parent ion of unknown substance 2 was found to fit to C 11 H 8 O 2 , and its secondary fragment structure may contain a benzene ring and two carbonyl groups, with a propylene structure connected to them through ring formation. From this, unknown substance 2 was presumed to be a menadione. The molecular ion peak of unknown substance 1 was found to fit to C 11 H 9 O 5 S - , only HSO 3 - was collected in the secondary fragments, and the missing part was consistent with unknown substance 2. Considering the most common derivatives of menadione, unknown substance 1 can be proposed to be menadione sodium bisulfite. Finally, we used menadione and menadione sodium bisulfite as reference substances in a comparative study. The same treatment method was used to prepare menadione, menadione sodium bisulfite reference solution, and enrofloxacin powder test solution. After UPLC-PDA detection, unknown substance 1 and menadione sodium bisulfite, unknown substance 2 and menadione, were found to have similar retention times and UV spectra. When the reference solution was added to the enrofloxacin powder test solution, the peak purity of the unknown substance did not change, and were all single peaks. UPLC-TOF-HRMS analysis revealed that the retention time of unknown substance 1 was consistent with that of sodium menadione bisulfite: compared to its accurate mass number in theory, the mass accuracy error was 1.0×10 -6 , and the matching degree of fragmentation information in the library was 100%. The retention time of unknown substance 2 was same as the menadione: compared to its accurate mass number in theory, the mass accuracy error was 0.6×10 -6 , and the matching degree of fragmentation information in the library was 99.7%. The structures of unknown substances 1 and 2 were confirmed. Menadione sodium bisulfite is known to participate in the synthesis of thrombin in the liver, and also promotes the formation of prothrombin, and accelerates coagulation. The indication of enrofloxacin powder (used for aquaculture) is the treatment of hemorrhage and sepsis in aquaculture animals such as fish and eel. The pharmacological effects of the two drugs correspond to each other, which can cause producers to take risks and add them illegally. With the strict supervision and severe restrictions on the addition of veterinary drugs, illegal additives are becoming more and more subtle. Conventional targeted analysis does not always meet the monitoring requirements. In this paper, the non-targeted analysis of unknown substances using UPLC-PDA combined with UPLC-TOF-HRMS is described in detail. The results may provide a technical reference for screening and identifying illegal additives in drugs, food, health care products, cosmetics, and pesticides.

Objective: To establish a micellar electrokinetic capillary chromatography-based method for identification and quantitative detection of interleukin-12 (IL-12) and analysis of its unfolding process., Methods: An uncoated fused-silica capillary (inner diameter 50 μm) with a total length of 48.5 cm (40 cm to the detector) was used for the experiment. The factors influencing the separation efficiency of IL-12 were analyzed, and a standard curve of IL-12 concentration was established. The mixture of IL-12 and anti-IL-12 antibody was incubated in a water bath at 38 ℃ for 40 min, and capillary electrophoresis was then performed under the same conditions. The results were compared with those of IL-12 and anti-IL-12 antibody to identify IL-12. IL-12 and dithiothreitol (DTT) were incubated at 60 ℃ in water bath for different lengths of times, and the unfolding process of IL-12 was analyzed based on electrophoresis results of IL-12 in different states., Results: A micellar capillary electrophoresis on-line sweep method was established with 80 mmol/L borate (pH=9.3) containing 30 mmol/L sodium dodecyl sulfate (SDS) as the buffer solution. This system showed a good linear relationship between the peak area and the mass concentration of IL-12 with a linear correlation coefficient of 0.9991 within the linear range of 2 to 120 ng/L. As the incubation time of IL-12 and DTT prolonged, the disulfide bond of IL-12 gradually opened and resulted in distinct changes in the protein peak., Conclusions: This capillary electrophoresis-based method is simple and sensitive for IL-2 analysis and allows rapid detection of changes in IL-12 content in the setting of tumors and analysis of the possible causes.

Objective: To analyze the whole genome sequences of Staphylococcus aureus strains from the sperm of infertile males and identify the gene which may induce the inhibition of sperm motility (ISM)., Methods: Twenty-two Staphylococcus aureus strains were isolated from the sperm of infertile males in the First Hospital Affiliated to Wenzhou Medical University and, according to the ability of ISM, divided into an ISM and a non-ISM group. Two strains most representative of the biological function of each group were selected, namely MJ015 from the ISM and MJ163 from the non-ISM group, and DNA extracted from them for whole genome sequencing. The data obtained were subjected to whole-genome sequence assembly and submitted to NCBI for annotation, with the accession number of CP038183 for MJ015 and CP038229 for MJ163. The whole genome sequences of MJ015 and MJ163 were compared in full detail using BRIG and Artemis software suite to identify the target gene., Results: The whole genome sequence of MJ015 was 2 784 836 bp in length, containing 2 plasmids, and that of MJ163 was 2 746 673, containing 1 plasmid, each with a 32.13%, 32.08% content of guanine-cytosine (GC), and annotated with 2 921 and 2 844 genes respectively. Comparison between the whole genome sequences of MJ015 and MJ163 revealed an almost 130 kb gap, in which a gene named sak was found to express a potential serum inhibition factor, whose transcription product was proved to be a differentially expressed protein in the two strains., Conclusions: The gene sak in MJ015 may play a key role in the inhibition of sperm mobility, but the inhibition intensity of its transcription product staphylococcus kinase has to be further studied.

To study and compare the medication regularities of traditional Chinese medicine formulas(TCMFs) for the six kinds of pains,namely abdominal pain,headache,epigastric pain,hypochondriac pain,heartache and backache,using a data-mining approach,in order to provide reference for relevant studies for the compatibility mechanism and new compound development of related TCMFs. A total of 2 443 TCMFs for pains were collected from the Dictionary of Chinese Medicine Prescriptions,and analyzed using the Apriori algorithm based on three indicators,namely confidence,lift and support,so as to study pivotal traditional Chinese medicines(TCMs) for each pain and their compatibility regularities. The results showed that deficiency tonifying medicines(such as Glycyrrhizae Radix et Rhizoma and Angelicae Sinensis Radix),Qi-regulating medicines(like Aucklandiae Radix) and blood circulation promoting medicines(like Chuanxiong Rhizoma) were commonly used TCMs for pains. However,there were many differences between drugs for various kinds of pains. For example,Magnoliae Officinalis Cortex was used frequently for abdominal pain and epigastric pain,while Saposhnikoviae Radix was used frequently for headache. The latent association rules with significant lift included Carthami Flos → Angelicae Sinensis Radix for abdominal pain,Astragali Radix → Glycyrrhizae Radix et Rhizoma for headache,Hordei Fructus Germinatus → Citri Reticulatae Pericarpium for epigastric pain,Gentianae Radix et Rhizoma → Bupleuri Radix for hypochondriac pain,and Caryophylli Flos → Moschus for backache. This study showed that based on the TCMFs from the Dictionary of Chinese Medicine Prescriptions,the data-mining approach can reveal the differences and similarities in the use of TCMs for the six kinds of pains,and discover the latent composition regularities of relevant TCMs.

Titanium dental implants have wide clinical application due to their many advantages, including comfort, aesthetics, lack of damage to adjacent teeth, and significant clinical effects. However, the failure of osseointegration, bone resorption, and peri-implantitis limits their application. Physical-chemical and bioactive coatings on the surface of titanium implants could improve the successful rate of dental implants and meet the clinical application requirements. This paper reviews the characteristics of surface modification of titanium implants from the aspects of physics, chemistry, and biology. Results provide information for research and clinical application of dental implant materials.

Most organisms contain glutamate dehydrogenase (E.C. 1.4.1.2-1.4.1.4). In eukaryotes, the enzyme is mainly present in mitochondria. This enzyme plays a vital role in the metabolism of nitrogen and carbon and the signaling pathway. Studies have found that glutamate dehydrogenase has a certain relationship with the occurrence and development of tumors, which is significant for tumor research, but reviews on its relationship with human tumors are rare. This review summarized the relationship between glutamate dehydrogenase and breast cancer, glioma, colorectal cancer and ovarian cancer, etc, thus providing assistance for related research.

With the comprehensive application and development of implant dentistry in recent years, multi-institutional data have supported a large number of clinical research findings. A consensus was gradually reached on the evaluation of the state and effect of implants and types of indicators that were selected after restoration. This study aims to examine the frequently used criteria to define treatment success in implant dentistry.

Dental implants represent the majority of treatment strategies used to replace missing teeth. However, peri-implant diseases caused by disturbance in peri-implant microbiological balance are among the reasons for implant failure. Since the 1980s, peri-implant microorganisms have been a hot research topic in dental microbiology. The bacterial ecology between the disease and health largely differs, which directly or indirectly increases the risk of peri-implant diseases. Accordingly, the determination of the 'core microbiome' of peri-implantitis and peri-implant mucositis is a key point of recent research.

Objective To investigate a protocol for the efficient differentiation of human induced pluripotent stem cells (hiPSCs) into insulin-producing cells (IPCs) in vitro. Methods Lysine-specific demethylase 1 (LSD1) gene was knocked down in hiPSCs by RNAi. A four-step method was performed to induce the differentiation of hiPSCs into IPCs. The differentiation efficiency of IPCs was analyzed by flow cytometry. Real-time quantitative PCR was used to detect the mRNA levels of LSD1, OCT4, SOX17, FOXA2, PDX1, PAX4, PAX6, HNF6, TCF1, NKX6.1, GLUT2, GK, insulin and MAFA. The expression and localization of PDX1 and insulin were determined by immunofluorescence technique. DTZ staining and transmission electron microscopy were used to observe the secretion and distribution of intracellular insulin-containing granules in IPCs. In addition, the yield of insulin and C-peptide of IPCs were tested by ELISA. Results Compared with the control, the LSD1 knockdown group showed a higher differentiation efficiency of IPCs and the mRNA expression of pancreatic islet β-cell development-related genes SOX17, PDX1, PAX4 and insulin were significantly up-regulated. IPCs from the LSD1 knockdown group co-expressed mature β-cell specific markers PDX1 and insulin. In the LSD1 knockdown group, IPCs released insulin as secretory vesicles in response to glucose stimuli, and the yield of insulin or C-peptide reached 1/6 of adult human islets (only 1/8 in the control group). Conclusion Knockdown of LSD1 can promote the efficient differentiation of hiPSCs into IPCs in vitro.

Etching treatment was performed on glass surface by dissociated nitrogen using anode layer linear ion source. The changes of glass surface after surface modification was analyzed and the effect of ionization voltage on surface roughness, refractive index and optical thickness was investigated. Accordingly, the relationship between Δ spectral characteristics of fixed wavelength changed angle of incidence and refractive index, Brewster angle, surface roughness and optical thickness was discussed through the comparison of Δ spectrum under different surface conditions based on spectroscopic ellipsometry. The results show that the shape of Δ spectrum near Brewster angle changes, the abrupt change of Δ spectrum shifts to larger angle, and the slope of Δ spectrum increase at the same time. Modeling and fitting analysis reveals that an optically denser layer was produced, the refraction index and Brewster angle increase and surface roughness decrease of glass caused by nitrogen ion beam etching treatment. However, the refraction remains consistence, and the depth of denser layer increase with increasing ionization voltage. The surface morphology analysis by AFM verified the leveling effect of glass surface by nitrogen ion beam. XPS measurement indicates the optional sputtering of glass surface by nitrogen ion beam, inferring the generation of denser layer arises from the compaction of ion beam. Furthermore, the general relationship between the characteristic of Δ spectrum and surface conditions of materials was theoretical derived and verified, and an evaluation of material surface variations was proposed. Such as the increase of abrupt change angle illustrates the increase of refractive index and Brewster angle, the increase of slope demonstrates the decrease of surface roughness, the increase of sharp corner of both sides evident the increase of optical thickness, vice-versa.

Objective: To explore material bases and neurobiological mechanisms of "Shen storing will" by observing learning and memory capacities and N-methyl-D-aspartic acid (NMDA) receptor expressions in Shen deficiency constitution (SDC) rats., Methods: Totally 40 SD rats were randomly divided into the model group, the Zuogui Pill (ZP) group, the Yougui Pill (YP) group, the blank control group (consisting of normal pregnant rats), 10 in each group. SDC young rat model (inherent deficiency and postnatal malnutrition) was prepared by the classic way of "cat scaring rat". Medication started when they were scared by cat. Rats in the ZP group and the YP group were administered by gastrogavage with ZP suspension 0.1875 g/mL and YP suspension 0.0938 g/mL respectively. Equal volume of normal saline was administered to rats in the blank control group and the model group by gastrogavage. All medication was given once per day, 5 days in a week for 2 consecutive months. Learning and memory capacities were detected by Morris water maze test. Expressions of NMDA receptor subunits NR2A and NR2B in hippocamus were detected by immunohistochemical method., Results: Compared with the blank control group, the latency period, total distance in Morris water maze test were longer in the model group (P < 0.05). All the aforesaid indices all decreased in the ZP group and the YP group, with statistical difference when compared with the model group (P < 0.05). The protein expressions of NR2A and NR2B in hippocamus were lower in the model group than in the blank control group (P < 0.05). But when compared with the model group, they were obviously higher in the ZP group and the YP group (P < 0.05)., Conclusions: SDC rats had degenerated learning and memory capacities and lowered NMDA receptor expressions. ZP and YP could up-regulate learning and memory capacities and NMDA receptor expressions, thereby improving deterioration of brain functions in SDC rats.

Objective: To test and estimate the forensic application of Goldeneye™ DNA ID 25A Kit., Methods: The kit was validated by a series of tests for accuracy, sensitivity, consistency, peak height balance, stability, and mixed samples through measured blood samples and other samples in routine casework., Results: The peak height balance of the different loci was ≥ 42%. The genotyping results of the positive control DNA was accurate. The complete STR genotyping result could be obtained from 0.125 ng positive control DNA., Conclusion: Goldeneye™ DNA ID 25A Kit is suitable for criminal cases and DNA database in forensic practice.

Objective: To investigate the effects of Mycobacterium tuberculosis heat-resistant antigen (MTB-HAg) and anti-TCR γδ monoclonal antibody (mAb) on the induction and differentiation of human IL-17-producing γδT cells under polarization culture conditions., Methods: From human peripheral blood mononuclear cells, the γδT cells were purified by magnetic-activated cell sorting (MACS) and then stimulated with MTB-HAg or anti-TCRγδ mAb and with or without anti-CD28 mAb, and cultured in the presence or absence of the cytokine cocktails (CK) (IL-1β, IL-6, TGF-β and IL-23) for 10 to 12 days. The polarized cultured γδT cells were collected and re-stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin for 6 hours, and the number of IL-17-producing cells was detected by ELISPOT., Results: The number of IL-17-producing cells (per 10⁵ γδT cells) in anti-TCRγδ combined with CK group was significantly higher than that in the anti-TCRγδ group. Meanwhile, the IL-17-producing γδT cell number in anti-TCRγδ combined with anti-CD28 group was lower than that in the anti-TCRγδ combined with anti-CD28 and CK group. The IL-17-producing γδT cell number of anti-TCRγδ combined with anti-CD28 and CK group was significantly higher than that in anti-TCRγδ combined with CK group, and that in MTB-HAg combined with anti-CD28 and CK group was significantly lower than that in anti-TCRγδ combined with anti-CD28 and CK group, but higher than that in MTB-HAg combined with anti-CD28 group., Conclusion: The CK (IL-1β, IL-6, TGF-β and IL-23) required for the differentiation of Th17 cells also induced the differentiation of IL-17⁺ γδT cells after pre-activated by MTB-HAg or anti-TCRγδ antibody, while anti-TCRγδ mAb showed the stronger stimulatory effect than MTB-HAg. In addition, CD28 co-stimulation enhanced the differentiation of IL-17 from activated γδT cells.

Objective: To study the antibiotic- and disinfectant-resistance features of and disinfectant-resistant gene distribution in Staphylococcus aureus (Sa) isolated from the urogenital tract of male patients with urogenital tract infection (UTI). total of 152 Sa isolates were collected from the urethral discharge specimens from male UTI patients. The minimum inhibition concentration (MIC) of antimicrobial agents and disinfectants commonly used against Sa were tested by standard ager dilution; the methicillin-resistant Sa (MRSA) isolates detected by cefoxitin disk diffusion and mecA gene amplification; Staphylococcal cassette chromosome mec (SCCmec) genotyping performed by multiplex PCR; the disinfectants gene qac (quaternary ammonium compound) amplified by PCR; and the clonal relatedness of qacA/B-positive MRSA isolates investigated by pulsed-field gel electrophoresis (PFGE)., Results: Out of the 152 Sa isolates, 91 (59.9%) were found to be MRSA. SCCmec genotyping showed SCCmec V to be the main type, accounting for 63.7% (58/91), with 8 (8.8%) isolates of SCCmec I, 2 (2.2%) isolates of SCCmec II, 19 (20.9%) isolates of SCCmec III, and 4 (4. 4%) isolates of SCCmec IV. The Sa isolates exhibited high rates of non-susceptibility to penicillin (95.4%) , erythromycin (72.4% ) , ciprofloxacin (42. 8%), and levofloxacin (44.7%), and a fairly high sensitivity to nitrofurantoin, teicoplanin, linezolid, and vancomycin. The MIC in the Sa isolates was 0. 25 -16 microg/ml for chlorhexidine; MIC50 and MIC90 were 2.0 and 4.0 microg/ml respectively for MRSA strains and both 1.0 microg/ml for MSSA strains. Out of the 152 Sa isolates, 72 (47.4%) harbored the qacA/B gene, 6 (3.9%) the smar (qacC + qacD) gene, 9 (5.9%) the qacE delta 1 gene, and 2 (1.3%) the qacH gene, but no qacG and qacJ genes were detected. PFGE analysis showed that the qacA/B-positive MRSA isolates were distributed, Conclusion: Clinical Sa isolates exhibited varied degrees of resistance to commonly used antibiotics, and in a polyclonal manner. some showed a robust tolerance to chlorhexidine. The main disinfectant-resistant gene is qacA/B. Antimicrobial agents and disinfectants should be used rationally according to clinicians.

Objective: To explore the reasonable radiotherapy range by analyzing the characteristics of supraclavicular lymph node metastasis in limited-stage small cell lung cancer (LS-SCLC)., Methods: From January 2005 to December 2011, patients of LS-SCLC were reviewed. Supraclavicular zone was further divided into five subgroups including para-recurrent laryngeal nerve (region I and region II ), para-internal jugular vein (region III ), supraclavicular region (region IV), as well as the other regions except for the mentioned above (region V). The characteristics of the lymph nodes in each region were analyzed., Results: The supraclavicular lymph node metastasis was found in 60 patients, with a positive rate of 34.5%. In multivariate Logistic regression analysis,intra-thoracic lymph node metastasis in the lymph node stations of level 2 and 3 were found to be the risk factors of supraclavicular lymph node metastasis (P = 0.006,P = 0.000). Our data suggests that the frequencies of metastasis in region I and III were much higher than those in the other areas.Among the sixty patients with supraclavicular lymph node metastasis, 95.0% were found at region I or III while the incidence of skip metastasis was only 5.0%., Conclusions: It is advisable to contain the bilateral supraclavicular nodes in patients with mediastinal lymph nodes metastasis to the level 2 or 3 for elective radiation target volume.The clinical target volume (CTV) exterior margin containing the outer margin of internal jugular vein may be suitable.

A new capillary zone electrophoresis method (CZE) has been established for the interaction analysis of protein C (PC) with a protein C activator (PCA) from Agkistrodon acutus venom. The analysis was performed on an uncoated fused-silica capillary with 75 microm i.d. and a total length of 60.2 cm (50 cm to the detector) with a buffer solution of 50 mmol/L Tris-HCl (pH 7.4) and 198 nm of wavelength. The factors which influence the separation of the PCA, such as buffer solution and ion concentration, and the interaction between the PCA and PC incubated for different times at 37.5 degrees C were studied. The linear range was from 10 to 300 mg/L. The limit of detection was 3 mg/L (S/N = 3). The relative standard deviation (RSD) for the migration time of the PCA was 0.56%. The RSD for the peak area was 3.8% (n = 6). The equal volumes of the PCA (200 mg/L) and PC (60 mg/L) were incubated for five minutes, at which their binding rate reached the maximum. And no hydrolyzed peptide chain from PC was found in the electropherogram. The PCA from Agkistrodon acutus venom could activate PC directly through changing the space conformation of PC. The method is simple, and highly sensitive with high resolution, and will provide important theoretical basis for the rapid detection of venom proteins and their activities in the future.

During the preparation of amino phosphoric chelating fiber, polypropylene grafted styrene, acetyl, amine series and amino phosphonic acid chelating fibers were certified by infrared spectrum, and the functionalization degree of raw fibers was studied. By the semi-qualitative method of infrared spectrum, the adsorption performance of indium and copper on amino phosphonic chelating fiber was also discussed. The results showed that (1) The peak at 1 116 cm(-1) was assigned to--P(ONa)2 in amino phosphonic acid chelating fiber. So the success of phosphorylation was verified. (2) During preparation, the phosphorylation effect of amino phosphonic acid chelating fiber could be reflected by the change of the peaks at 1 056 and 1 110 cm(-)1. (3) After adsorption of In3+ on amino phosphonic acid chelating fiber, the new forming N-In coordination key was absorbed strongly at the bands of 1 000-1 200 cm(-1) and at 1 107, 699 and 617 cm(-1). After adsorption Cu2+ on amino phosphonic acid chelating fiber two new strong and wide peaks were found at 1 110 and 618 cm(-1), respectively. (4) Through the area change of the bands at 1 200-900 and 600 cm(-1), the adsorption performance of indium and copper on amino phosphonic acid chelating fiber was compared.

Landscape pattern indices are the commonly used tools for the quantitative analysis of landscape pattern. However, the traditional 2D landscape pattern indices neglect the effects of terrain on landscape, existing definite limitations in quantitatively describing the landscape patterns in mountains areas. Taking the Qixia City, a typical mountainous and hilly region in Shandong Province of East China, as a case, this paper compared the differences between 2D and 3D landscape pattern indices in quantitatively describing the landscape patterns and their dynamic changes in mountainous areas. On the basis of terrain structure analysis, a set of landscape pattern indices were selected, including area and density (class area and mean patch size), edge and shape (edge density, landscape shape index, and fractal dimension of mean patch), diversity (Shannon's diversity index and evenness index) , and gathering and spread (contagion index). There existed obvious differences between the 3D class area, mean patch area, and edge density and the corresponding 2D indices, but no significant differences between the 3D landscape shape index, fractal dimension of mean patch, and Shannon' s diversity index and evenness index and the corresponding 2D indices. The 3D contagion index and 2D contagion index had no difference. Because the 3D landscape pattern indices were calculated by using patch surface area and surface perimeter whereas the 2D landscape pattern indices were calculated by adopting patch projective area and projective perimeter, the 3D landscape pattern indices could be relative accurate and efficient in describing the landscape area, density and borderline, in mountainous areas. However, there were no distinct differences in describing landscape shape, diversity, and gathering and spread between the 3D and 2D landscape pattern indices. Generally, by introducing 3D landscape pattern indices to topographic pattern, the description of landscape pattern and its dynamic change would be relatively accurate.

Objective: To determine the effect of exogenous GM3 on proliferation, apoptosis and VEGF expression in human lung adenocarcinoma cell line A549 cells., Methods: A549 cells were treated with GM3 at different concentrations for 48 hours. MTT assay was used to detect the cell proliferation and flow cytometry was applied to analyze cell apoptosis. RT-PCR was used to detect the expression level of VEGF mRNA and confocal laser scanning microscopy was applied to observe the localization and fluorescence intensity of VEGF., Results: Comparing with the control, being treated with higher than 10 µmol/L GM3 significantly inhibited A549 cell proliferation (P < 0.05), and the suppressive effect could be enhanced following increasing doses. The IC(50) was 412 µmol/L. Comparing with the control, being treated with higher than 40 µmol/L GM3 significantly promoted the apoptotic rate of A549 cells (P < 0.05). Comparing with the control, being treated with higher than 40 µmol/L GM3 significantly decreased the VEGF mRNA level of A549 cells (P < 0.05), and the fluorescence intensity of VEGF distinctly weakened., Conclusions: Exogenous ganglioside GM3 can inhibit the proliferation, promote apoptosis, and down-regulate the VEGF expression level in A549 cells. This may be considered as two mechanisms of GM3 for its anti-tumor effect by modulating cell apoptosis and angiogenesis.

Objective: To investigate the preparation method of polystyrene core-poly (acrylamide-acrylic acid) shell fluorescent microspheres., Methods: The polystyrene core-poly (acrylamide-acrylic acid) shell (P-(St-co-AAM)) fluorescent microspheres were prepared using fluorescent microspheres as the core and acrylamide/acrylic as polymerization monomer. Reaction conditions affecting the morphology of core-shell structure including feeding mode, initiator, cross linker, pH, concentration and swelling were studied., Result: Fluorescent microscopy showed that the relatively uniform particle sizes were distributed in a range of 7-8 μm. Fourier transform infra-red spectroscopy (FT-IR) proved the existence of poly (acrylamide-acrylic acid) shell and amide group on the surface. The optimal conditions for seeding polymerization: azobisisobutyronitrile was used as the initiator in the absence of cross linker, after a 40 h swelling treatment by using alcohol with the appropriate reaction temperature (70 degree), reaction time (3 h) and pH(6-7). The average dispersion and stability were 25.14 % and 90.21%, respectively. The fluorescein release percentage was kept stable at approximately 30% after 40 h., Conclusion: The fluorescent microspheres prepared by this method have core-shell structure and satisfactory fluorescence properties with good dispersion and stability.