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[Effects of Mycobacterium tuberculosis antigen and anti-TCRγδ antibody on IL-17 production of human γδT cells in polarization culture].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2015 Feb; Vol. 31 (2), pp. 226-30. - Publication Year :
- 2015
-
Abstract
- Objective: To investigate the effects of Mycobacterium tuberculosis heat-resistant antigen (MTB-HAg) and anti-TCR γδ monoclonal antibody (mAb) on the induction and differentiation of human IL-17-producing γδT cells under polarization culture conditions.<br />Methods: From human peripheral blood mononuclear cells, the γδT cells were purified by magnetic-activated cell sorting (MACS) and then stimulated with MTB-HAg or anti-TCRγδ mAb and with or without anti-CD28 mAb, and cultured in the presence or absence of the cytokine cocktails (CK) (IL-1β, IL-6, TGF-β and IL-23) for 10 to 12 days. The polarized cultured γδT cells were collected and re-stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin for 6 hours, and the number of IL-17-producing cells was detected by ELISPOT.<br />Results: The number of IL-17-producing cells (per 10⁵ γδT cells) in anti-TCRγδ combined with CK group was significantly higher than that in the anti-TCRγδ group. Meanwhile, the IL-17-producing γδT cell number in anti-TCRγδ combined with anti-CD28 group was lower than that in the anti-TCRγδ combined with anti-CD28 and CK group. The IL-17-producing γδT cell number of anti-TCRγδ combined with anti-CD28 and CK group was significantly higher than that in anti-TCRγδ combined with CK group, and that in MTB-HAg combined with anti-CD28 and CK group was significantly lower than that in anti-TCRγδ combined with anti-CD28 and CK group, but higher than that in MTB-HAg combined with anti-CD28 group.<br />Conclusion: The CK (IL-1β, IL-6, TGF-β and IL-23) required for the differentiation of Th17 cells also induced the differentiation of IL-17⁺ γδT cells after pre-activated by MTB-HAg or anti-TCRγδ antibody, while anti-TCRγδ mAb showed the stronger stimulatory effect than MTB-HAg. In addition, CD28 co-stimulation enhanced the differentiation of IL-17 from activated γδT cells.
- Subjects :
- Adolescent
Adult
Cell Differentiation
Female
Humans
Interleukin-23 immunology
Interleukin-6 immunology
Male
T-Lymphocyte Subsets cytology
Th17 Cells cytology
Transforming Growth Factor beta immunology
Tuberculosis microbiology
Young Adult
Antibodies, Monoclonal immunology
Antigens, Bacterial immunology
Interleukin-17 immunology
Mycobacterium tuberculosis immunology
Receptors, Antigen, T-Cell, gamma-delta immunology
T-Lymphocyte Subsets immunology
Th17 Cells immunology
Tuberculosis immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 31
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 25652865