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ObjectiveTo investigate the characteristics and diversity of paracancerous tissue microbiota among esophageal squamous cell carcinoma (ESCC) patients from different regions of Fujian province for providing evidences to ESCC prevention. Methods Totally 120 ESCC patients were recruited after radical surgery at two municipal hospitals in two cities of Fujian province from February 2013 through October 2017. All the participants were pathologically diagnosed primary ESCC cases with local residential history of more than 10 years and with intact para-cancerous tissue/exact tumor stage/complete medical records and but without infectious disease/other primary malignant, without antimicrobial therapy/radiotherapy/ chemotherapy during two months before the surgery and without specific medicine use during one month before the surgery. A face-to-face questionnaire interview was conducted in the participants. Double-ended sequencing with 16S rRNA V3-V4 variable region was performed for para-cancerous tissue specimens. The paracancerous tissue microbiota diversity and composition were analyzed with QIIME2019.4. Multivariate unconditional logistic regression was used to assess the association of residential region with patients' paracancerous tissue microbiota. Results Of the 120 participants, 105 were from 4 prefectures (Zhangzhou,Fuzhou, Putian and Quanzhou prefecture) and only 15 participants from other 5 prefectures of the province. There were no significantly differences in gender, age, smoking, alcohol and tea consumption, sampling season and tumor site among the participants from various regions (all P > 0.05); while the alpha- and beta-diversity in paracancerous tissue microbiota were significantly different among the participants from various regions (both P < 0.05). The abundance of para-cancerous esophageal microbiota of 15 phyla, 22 classes, 28 orders, 48 families, 71 genera, and 85 species were calculated with QIIME 2019.4. After adjusting for confounding factors including gender, age, smoking, alcohol consumption, tea consumption, sampling seasons and tumor location, the multivariate unconditional logistic regression analysis of dominant microbiota showed that the abundance of order Rickettsiales and family mitochondria detected in the participants from Fuzhou were higher than those in the participants from Zhangzhou; while the abundance of order Sphingomonadales, family Ruminococcaceae and Sphingomonadaceae, genus Faecalibacterium and Sphingomonas detected in the participants from Fuzhou were lower than those in the participants from Zhangzhou; the abundance of class Actinobacteria, order Caulobacterales and Sphingomonadales, family Ruminococcaceae, Caulobacteraceae and Sphingomonadaceae, and genus Sphingomonas in detected in the participants from Putian were lower than those in the participants from Zhangzhou; the abundance of phylum OD1, order Rickettsiales and family mitochondria in the participants from Quanzhou were higher than those in the participants from Zhangzhou; the abundance of order Coriobacteriales, Caulobacterales and Sphingomonadales, family Ruminococcaceae, Caulobacteraceae, Sphingomonadaceae, and Coriobacteriaceae, and genus Faecalibacterium in the participants from Quanzhou were lower than those in the participants from Zhangzhou; the abundance of class Coriobacteriia, order Coriobacteriales and Caulobacterales, family Ruminococcaceae, Caulobacteraceae and Coriobacteriaceae, and genus Faecalibacterium in the participants from other regions were lower than those in the participants from Zhangzhou. After adjusting for gender, age, smoking, alcohol consumption, tea consumption, sampling season and tumor site, the multivariate unconditional logistic regression analysis on rare microbiota showed that the abundance of phylum Verrucomicrobia, class Verrucomicrobiae, order Verrucomicrobiales, family Verrucomicrobiaceae, genus Roseburia, Mycoplana and Akkermansia, and species muciniphila were more likely to be lower in the participants from Fuzhou in comparison with the participants from Zhangzhou; the abundance of phylum Verrucomicrobia, class Verrucomicrobiae, order Verrucomicrobiales, family Verrucomicrobiaceae, genus Blautia, Roseburia, Mycoplana and Akkermansia, and species Longicatena and Muciniphila were more likely to be lower in the participants from Putian, Quzhou and other regions compared to the participants from Zhangzhou. Conclusion Residential region is an influencing factor for paracancerous tissue microbiota among ESCC patients.

The experiment was to study the effect of adding different levels of compound acidifiers at different storage temperatures on diversity and composition of eel feed microflora. The commercial feed of eel with compound acidifiers of 0, 3.0 and 6.0 g/kg was stored at room temperature (20 ℃ ) and high temperature (40 ℃ ) for 60 d, respectively. The 16S rDNA method was used for high-throughput sequencing analysis of the feed microbial community α diversity and differential bacteria, five replicates were set for each group of feed. The results showed that under normal temperature, the microbial population of eel food with different levels of compound acidifiers α diversity index had no significant difference (P>0.05). Under high temperature, adding 6.0 g/kg compound acidifier to eel food bacteria α diversity and relative abundance of potential probiotics were significantly higher than other groups (P<0.05). There was no significant difference in eel food microflora at phyla level under different temperatures (P>0.05). The study indicates that adding compound acidifier has beneficial regulation on eel food bacteria, and the effect of adding higher level compound acidifier at high temperature is better. [ABSTRACT FROM AUTHOR]

Objective: To analyze the correlation between nasal mucosal microbiota diversity and the pathogenesis and prognosis of chronic sinusitis. Methods: A total of 80 patients with chronic sinusitis（CRS） admitted to Second Affiliated Hospital of Suzhou University were selected as the research group, and 80 patients with chronic dacryocystitis and nasal septum deviation without sinus inflammation admitted to our hospital during the same period were selected as the control group, nasal secretory specimens were collected under nasal endoscopic guidance by nasal swab, and matrix-assisted laser desorption ionization classification time mass spectrometry, anaerobic bacterial culture and common bacterial culture were performed to compare the differences in nasal mucosal flora between groups. Patients with chronic sinusitis were followed up for 6 months, and nasal secretions were collected again to detect microflora, and the patients were divided into relapse group（21 cases） and non-recurrence group（59 cases） according to whether the patients had relapse. Compare the diversity of nasal microbiota between groups. Results: There were no significant differences in mean relative abundance（MRA） between the two groups of preoperative phylum Microbacterium verrucobacterium, Cyanobacterium phylum, Phylum Laubia mlaus, Porphyromonas species, Enterococcus species, Fusobacterium species, Enterobacter species, Enterobacter species, Erythrobacterium species, Ralstonia species, Bacteroides and Streptococcus species（ P >0.05）. The MRA of Acidobacterium, Proteobacteria, Actinomycetes MRA, Moraxia, Cyanobacterium, Corynebacterium and Staphylococcus were significantly lower than those of the control group（ P <0.05）, and the MRA of Escher-Shigella species, Fusobacterium, Bacteroides, Firmicutes, Neisseria, Pseudomonas, Haemophilus and Lactobacillus was significantly higher than that of the control group（ P <0.05）, and there was no significant difference in MRA at the level of nasal flora and genus before and after surgery in the relapsed group （P >0.05）. The MRA of Bacteroides after surgery was significantly lower in the non-recurrent group than that before surgery（ P <0.05）, the MRA of Corynebacterium and Actinomycetes was higher significantly than that of preoperative（ P <0.05）, and there was no significant difference in MRA of other species and phylum（ P >0.05）. Conclusion: The onset of CRS is related to nasal mucosal dysbacteria, and whether the dysbacteriosis improves after surgery is correlated with the prognosis of patients., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)