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Objective: To optimize the enzymatic hydrolysis process of Pueraria protein and study its antioxidant properties. Methods: DPPH radical scavenging ability and DH were used as the index to screen the best hydrolytic protease. Base on the single factor experiments, the Box-Behnken response surface design was used to optimize the enzymatic hydrolysis process of Pueraria protein, and the antioxidant properties of the optimum hydrolysate in vitro was analyzed. Results: The optimal hydrolysis conditions for alkaline protease were as follows: Temperature 55 ℃, pH9 and enzyme substrate ratio 2%. Under these conditions, the IC50 of DPPH·, ABTS+·, and ·OH radicals were 0.15, 0.38, 1.41 mg/mL and a reduction capacity of 0.553. Conclusion: Under these conditions, Pueraria protein hydrolysate had remarkable antioxidant properties.

Background and objective Fas/FasL is a member of Tumor Necrosis Factor (TNF) super family and related to tumor cell apoptosis. It is hypothesis by forward study that activated lymphocytes is more sensitive with Fas/FasL due to up-regulation of FasL expression, so it can be inverse killedly and cleared by tumor cell. The aim of this investigate is to study the fuction of FasL gene and gene therapy of lung cancer by to down-regulationg the FasL gene expression with a siRNA expression plasmid in lung cancer cell A549 as well as its inverse killing effect between activated T lymphocytes and lung cancer cell A549. Methods Potential RNAi oligonucleotides of FasL was designed and synthesized according to appropriate web site. Then a FasL siRNA plasmid was constructed using a pGCsi-U6 vector.The plasmid was sequenced to confirm the inserted sequence. Western blot analysis was used to assess the levels of FasL proteins after the constructed plasmids have been transfected into A549 cells. Results It was confirmed by sequencing that the plasmid was constructed successfully. The result of Western blot clearly showed that FasL siRNA plasmid inhibited FasL expression in A549 cells. Conclusion The construct of FasL siRNA plasmid is successful. FasL protein expression of A549 cell is effectively inhibited by RNAi .

In order to prevent the occurrence of water inrush and sand burst during coal mining in the upper group of Maiduoshan Coal Mine, drainage tests were carried out on the aquifer of Yan’an Formation between 1 and 2 coal seams through underground water drainage holes and hydro-geological observation holes, and the hydro-geological parameters were obtained, and the underground water flow field was simulated by software. The results show that the aquifer of Yan’an Formation receives direct recharge from the aquifer of lower Zhiluo Formation, that is, the two groups of the aquifer have close hydraulic connection. There is no abnormal area of high water level that cannot be lowered in the aquifer area, so the method of long-time drainage can be adopted to prevent water inrush and sand bursting from roof.