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Optimization of Enzymatic Hydrolysis Process of Pueraria Protein by Response Surface Methodology and Its Antioxidant Properties in Vitro
- Source :
- Shipin gongye ke-ji, Vol 43, Iss 24, Pp 197-204 (2022)
- Publication Year :
- 2022
- Publisher :
- The editorial department of Science and Technology of Food Industry, 2022.
-
Abstract
- Objective: To optimize the enzymatic hydrolysis process of Pueraria protein and study its antioxidant properties. Methods: DPPH radical scavenging ability and DH were used as the index to screen the best hydrolytic protease. Base on the single factor experiments, the Box-Behnken response surface design was used to optimize the enzymatic hydrolysis process of Pueraria protein, and the antioxidant properties of the optimum hydrolysate in vitro was analyzed. Results: The optimal hydrolysis conditions for alkaline protease were as follows: Temperature 55 ℃, pH9 and enzyme substrate ratio 2%. Under these conditions, the IC50 of DPPH·, ABTS+·, and ·OH radicals were 0.15, 0.38, 1.41 mg/mL and a reduction capacity of 0.553. Conclusion: Under these conditions, Pueraria protein hydrolysate had remarkable antioxidant properties.
Details
- Language :
- Chinese
- ISSN :
- 10020306
- Volume :
- 43
- Issue :
- 24
- Database :
- Directory of Open Access Journals
- Journal :
- Shipin gongye ke-ji
- Publication Type :
- Academic Journal
- Accession number :
- edsdoj.90b8415fdd3b4775a72828b93f29957f
- Document Type :
- article
- Full Text :
- https://doi.org/10.13386/j.issn1002-0306.2021100147