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Objective: To explore the damage of cisplatin to the structure and function of cajal interstitial cells(ICCs)in gastric tissue and the protective effect of curcumin. Methods: Adult kunming male mice were randomly divided into control group (NC), cisplatin group(DDP)and cisplatin+curcumin group(DDP+Cur) with 10 mice in each group. Curcumin(200 mg/kg/d)mixed with the suspension by intragastric administration for a total period of 15 days. Intraperitoneal injection of cisplatin (2 mg/kg/d) was started from the last 5th day, for a total of 5 days. The weight gain and loss of each mouse on the last 5 days were calculated, and the gastric emptying rate of each mouse was measured after stopping the drug for 24 h. The ultrastructure of ICC in gastric tissue was detected by electron microscope and the expression ofAno1 protein and mRNAin gastric tissue, which specifically responded to the changes of ICCs, were detected also. Results: The weight and gastric emptying rate of mice were significantly decreased after injection of cisplatin (P<0.01). Compared with cisplatin group, cisplatin+curcumin group had less weight loss (P<0.01) and gastric emptying rate were significantly increased(P<0.05). After injection of cisplatin, ICCs in gastric tissue were damaged, especially the gap junctions between peripheral nerve and muscular cells were winden or even broken, and curcumin could alleviate these damage. In addation, the expression of Ano1mRNA and protein in cisplatin group decreased (P<0.01), while it was improved significantly in cisplatin+curcumin group (P<0.05). Conclusion: Curcumin can reduce cisplatin-induced structural damage of ICC by increasing the expression of Ano1 and incraesing the function of slow waves generation. [ABSTRACT FROM AUTHOR]

Objective · To investigate the possible factors affecting the gastric emptying rate in patients with type 2 diabetes mellitus. Methods · 94 type 2 diabetic patients treated at the Department of Endocrinology of Renji Hospital affiliated to Shanghai Jiao Tong University School of Medicine from September 2013 to March 2014 were enrolled. The half gastric emptying time (T1/2) and retention at 120 min (R120 min) were measured. Results · Female patients had longer T1/2 (P=0.000) and higher R120 min (P=0.000) than male patients. The differences in age, duration of diabetes, BMI, blood glucose level, chronic diabetic complications, and gastrointestinal symptoms between normal and delayed gastric emptying groups were not statistically significant. Acarbose had no effect on the gastric emptying rate in type 2 diabetic patients. Conclusion · The gastric emptying rate in type 2 diabetic patients is associated with the gender and has no significant correlation with blood glucose level, chronic diabetic complications, gastrointestinal symptoms, and acarbose. [ABSTRACT FROM AUTHOR]