姜黄素对顺铂所致胃组织ICC 损伤的保护作用及机制.

Objective: To explore the damage of cisplatin to the structure and function of cajal interstitial cells(ICCs)in gastric tissue and the protective effect of curcumin. Methods: Adult kunming male mice were randomly divided into control group (NC), cisplatin group(DDP)and cisplatin+curcumin group(DDP+Cur) with 10 mice in each group. Curcumin(200 mg/kg/d)mixed with the suspension by intragastric administration for a total period of 15 days. Intraperitoneal injection of cisplatin (2 mg/kg/d) was started from the last 5th day, for a total of 5 days. The weight gain and loss of each mouse on the last 5 days were calculated, and the gastric emptying rate of each mouse was measured after stopping the drug for 24 h. The ultrastructure of ICC in gastric tissue was detected by electron microscope and the expression ofAno1 protein and mRNAin gastric tissue, which specifically responded to the changes of ICCs, were detected also. Results: The weight and gastric emptying rate of mice were significantly decreased after injection of cisplatin (P<0.01). Compared with cisplatin group, cisplatin+curcumin group had less weight loss (P<0.01) and gastric emptying rate were significantly increased(P<0.05). After injection of cisplatin, ICCs in gastric tissue were damaged, especially the gap junctions between peripheral nerve and muscular cells were winden or even broken, and curcumin could alleviate these damage. In addation, the expression of Ano1mRNA and protein in cisplatin group decreased (P<0.01), while it was improved significantly in cisplatin+curcumin group (P<0.05). Conclusion: Curcumin can reduce cisplatin-induced structural damage of ICC by increasing the expression of Ano1 and incraesing the function of slow waves generation. [ABSTRACT FROM AUTHOR]