1. Stable expression of the extracellular domains of rabbit recombinant CD5: development and characterization of polyclonal and monoclonal antibodies
- Author
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Pospisil, Richard, Obiakor, Harold, Newman, Barbara A., Alexander, Cornelius, and Mage, Rose G.
- Subjects
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IMMUNOGLOBULINS , *MONOCLONAL antibodies , *LYMPHOCYTES - Abstract
Abstract: Previous studies in our laboratory suggested that there was positive selection of B cells during early development in the appendix of normal and VH mutant (ali/ali) rabbits. Preferential expansion and survival of B lymphocytes was affected by the Ig VH frameworks 1 and 3 sequences expressed on the cell surface. We demonstrated a specific interaction between rabbit CD5 and the V region of rabbit heavy chains and suggested that CD5 is a potential selecting ligand for B-cell surface immunoglobulin framework region sequences. To further investigate the role of CD5 in rabbit B-cell selection and survival we prepared recombinant constructs and obtained stable expression of the three scavenger receptor cysteine-rich (SRCR) extracellular domains of rabbit CD5. Here we describe the production and purification of this expressed recombinant CD5 protein, polyclonal antibody obtained by immunization of a goat and initial production and characterization of specific mAbs against peptides selected from each sequenced SRCR domain. [Copyright &y& Elsevier]
- Published
- 2005
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