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Stable expression of the extracellular domains of rabbit recombinant CD5: development and characterization of polyclonal and monoclonal antibodies
- Source :
-
Veterinary Immunology & Immunopathology . Feb2005, Vol. 103 Issue 3/4, p257-267. 11p. - Publication Year :
- 2005
-
Abstract
- Abstract: Previous studies in our laboratory suggested that there was positive selection of B cells during early development in the appendix of normal and VH mutant (ali/ali) rabbits. Preferential expansion and survival of B lymphocytes was affected by the Ig VH frameworks 1 and 3 sequences expressed on the cell surface. We demonstrated a specific interaction between rabbit CD5 and the V region of rabbit heavy chains and suggested that CD5 is a potential selecting ligand for B-cell surface immunoglobulin framework region sequences. To further investigate the role of CD5 in rabbit B-cell selection and survival we prepared recombinant constructs and obtained stable expression of the three scavenger receptor cysteine-rich (SRCR) extracellular domains of rabbit CD5. Here we describe the production and purification of this expressed recombinant CD5 protein, polyclonal antibody obtained by immunization of a goat and initial production and characterization of specific mAbs against peptides selected from each sequenced SRCR domain. [Copyright &y& Elsevier]
- Subjects :
- *IMMUNOGLOBULINS
*MONOCLONAL antibodies
*LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 01652427
- Volume :
- 103
- Issue :
- 3/4
- Database :
- Academic Search Index
- Journal :
- Veterinary Immunology & Immunopathology
- Publication Type :
- Academic Journal
- Accession number :
- 15670335
- Full Text :
- https://doi.org/10.1016/j.vetimm.2004.09.030