1. Microbial diversity in various types of paper mill sludge: identification of enzyme activities with potential industrial applications
- Author
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Manel Ghribi, Marc Beauregard, and Fatma Meddeb-Mouelhi
- Subjects
0106 biological sciences ,0301 basic medicine ,01 natural sciences ,Bioplastic ,03 medical and health sciences ,chemistry.chemical_compound ,Hydrolysis ,Bioremediation ,010608 biotechnology ,Lignin ,Pulping liquors ,Dyes ,Multidisciplinary ,Bacteria ,biology ,business.industry ,Research ,food and beverages ,Paper mill ,biology.organism_classification ,Pulp and paper industry ,16S ribosomal RNA ,Enzymes ,Biotechnology ,030104 developmental biology ,chemistry ,business ,Kraft paper - Abstract
This study is the first comprehensive investigation of enzyme-producing bacteria isolated from four sludge samples (primary, secondary, press and machine) collected in a Kraft paper mill. Overall, 41 strains encompassing 11 different genera were identified by 16S rRNA gene analysis and biochemical testing. Both biodiversity and enzymatic activities were correlated with sludge composition. Press sludge hosted the largest variety of bacterial strains and enzymatic activities, which included hydrolytic enzymes and ligninolytic enzymes. In contrast, strains isolated from secondary sludge were devoid of several enzymatic activities. Most strains were found to metabolize Kraft liquor at its alkaline pH and to decolorize industrial lignin-mimicking dyes. Resistance to lignin or the ability to metabolize this substrate is a prerequisite to survival in any paper mill sludge type. We demonstrate here that the bacterial strains found in a typical Kraft paper mill represent a source of potential novel enzymes for both industrial applications and bioremediation. Electronic supplementary material The online version of this article (doi:10.1186/s40064-016-3147-8) contains supplementary material, which is available to authorized users.
- Published
- 2016