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[Objective] By standardizing, expediting, and automating the evaluation of integrated farmland soil fertility, the present paper aimed to optimize crop planting patterns and make use of land resources rationally. [Method] Taking the tobacco planting areas of Fuchuan Yao Autonomous County as examples, 1038 sample points from Guangxi tobacco planting areas were selected as training data. Based on the consideration of soil conditions, climate environment, and topography, 15 indicators were chosen to calculate the integrated soil fertility index. The Stacking algorithm was applied to establish a regression prediction model for evaluating the integrated fertility of the study area. [Result] The Stacking02 model based on six key factors exhibited excellent performance on the test set, with mean absolute error, mean squared error, and R2 coefficient of determination of 0.0066, 0.0084, and 0.9529, respectively. The Stacking02 model was used to assess the integrated fertility of the study area, showing that the northern part of study area was better than the southern part, with a higher proportion of high-quality production areas. Low comprehensive fertility index regions had relatively high soil pH values and low total nitrogen and available potassium contents. [Conclusion] Compared to primary learners, the performance of the Stacking model improved. The Stacking02 model established using six key factors demonstrated excellent generalization performance, simplifying the calculation process and saving computational power. The same method can also be used to evaluate the soil integrated fertility of the study area. It suggests that this method should be extended to the comprehensive fertility evaluation of other regions and crops’ soils to develop targeted crop utilization schemes. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to screen leguminous plants suitable for improving the soil of bauxite mine reclaimed soil and provide a reference basis for sustainable agricultural development in mining areas. [Method] Seven legumes, including Sesbania cannabina, Vicia sativa, Astragalus sinicus, V. villosa, Medicago sativa, Stylosanthes guianensis and Coronilla varia, were planted in the reclaimed soil of the Pingguo bauxite mine in Guangxi, and the biomass, presence of root nodules, nutrient content, nitrogen fixation efficiency, biological nitro- gen fixation, soil nutrients, calcium and magnesium ion content, total aluminum and active aluminum content of each legume were determined. The legumes suitable for planting in bauxite mine reclamation soil were screened through principal component analysis, and a partial least squares path model (PLS-PM) was established to explore the factors that affected the biological nitrogen fixation of legumes in bauxite mine reclamation soil. [Result] Among the seven legumes, A. sinicus had the highest below-ground biomass(1.961 g/pot) and above-ground bio-mass (0.344 g/pot), C. varia had the lowest below-ground biomass (0.387 g/pot) and above-ground biomass (0.099 g/pot) while Sesbania had the highest N(7.98% ), P(2.999 g/kg), and K(19.828 g/kg) contents. In terms of nodulation, only Sesbania, A. sinicus, and C. varia had root nodules, while the rest of the legumes did not nodulate. Among the three nodulated legumes, S. cannabina had the highest nitrogen fixation efficiency (80.01%) and biological nitrogen fixation (0.077 g/pot). Principal component analysis of three leguminous plants based on evaluation factors including plant nutrient content, biomass, biological nitrogen fixation, and nitrogen fixation efficiency as evaluation factors showed that comprehensive score of S. cannabina repens was the highest (2.30 points). PLS-PM pathway analysis showed that the biological nitrogen fixation of legumes in bauxite mine reclamation soil was directly influenced by plant nutrient content and soil active aluminum and pH, soil nutrients, and types of plants indirectly influenced the biological nitrogen fixation of legumes by affecting plant nutrient content and soil active aluminum. [Conclusion] S. cannabina, A. sinicus, and C. varia are capable of nodulating and improving the pH, available K, ac- tive aluminum, organic matter content, and biological nitrogen fixation of the reclaimed soil in bauxite mines. Based on the comprehensive e- valuation, S. cannabina is considered as the most suitable legume plant for planting in the reclaimed soil of bauxite mines and can be promo- ted and applied as a legume plant for nitrogen fixation and soil improvement in bauxite mining areas. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to establish the evaluation method of heat resistance of leek resources, select heat-resistant leek varieties, and solve the problem of shortage of heat-resistant leek varieties in Guangxi.【Method】Taking three kinds of local germplasm resources of leek（marked as A, B, C, D, E, F, G, H and I, respectively） of Guangxi large leaf leek, wide leaf leek and thin leaf leek collected from the general survey of germplasm resources by Ministry of Agricultural and Rural Affairs（2015-2020） as test materials, the changes of the maximum growth length of leek leaves, the newly increased aboveground biomass and the contents of chlorophyll, proline and malondialdehyde after 7 days of high temperature stress at 38 ℃ were measured. Combined with field natural high temperature stress（field group） actual heat resistance performance, the heat resistance of different types of leek was comprehensively evaluated by multivariate statistical analysis（variance analysis, principal component analysis, membership function method and cluster analysis） methods.【Result】Under high temperature stress of 38 ℃,the maximum leaf growth length and the newly increased biomass of 9 leek germplasms of the three types in Guangxi were extremely significantly decreased compared with their proper temperature control（CK）（P＜0.01）.The largest decline was the type of large-leaved leek, and the smallest decline was the type of fine-leaved leek. Chlorophyll content decreased to different degrees, of which the largest decline was the type of leek with large leaves, and the smallest decline was the type of leek with thin leaves. The content of MDA increased to different degrees, of which the largest increase was the type of leek with large leaves, and the smallest increase was the type of leek with thin leaves. Pro content increased to different degrees, of which, the largest increase was the type of leek with thin leaves, and the smallest increase was the type of leek with large leaves. The comprehensive evaluation results showed that the heat tolerance of the three kinds of local leek germplasm resources of leek in Guangxi was ranked as follows: leek with thin leaves＞leek with broad leaves＞leek with large leaves. Germplasm G（No.2016451159） and Germplasm I（No.2016453604） had the strongest heat tolerance among the nine leek germplasms, and Germplasm C（No.SC20191200） had the weakest heat tolerance.【Conclusion】The maximum growth length of leaves, new aboveground biomass and the content of chlorophyll, Pro and MDA contents were used as the evaluation indicators of heat tolerance of leek germplasm resources, the heat tolerance of three kinds of leek germplasm resources in Guangxi can be ranked as follows: Leek with thin leaves＞ leek with broad leaves＞leek with large leaves by variance analysis, principal component analysis, membership function and cluster analysis. Germplasm G and germplasm I were determined to be more heat-resistant, while germplasm C was the least heat-resistant. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to analyze the effects of pesticides fertilizer application on the occurrence of diseases and insect pests in sugarcane during inter-tillage period, and their production effects at jointing and maturity stages, so as to provide reference basis for scientific application of pesticide fertilizer in sugarcane.【Method】In this study, sugarcane cultivar Guiliu 05/136 planted in winter was taken as the research material. Referring to the production standard of sugarcane with high yield and high sugar in Guangxi, six different kinds of pesticides fertilizers with nutrient ratios and pesticides components were applied to sugarcane production during the inter-tillage period（from tillering stage to late tillering stage） as different treatments, and they were named as A,B,C,D,E and F,respectively. From May to October, the conditions of major plant diseases and insect pests in sugarcane seedlings under different treatments, and the growth rate of sugarcane plants at elongation stage were investigated. The yield traits were investigated at maturity, and the agricultural output value of sugarcane under different treatments were also analyzed.【Result】Application of pesticides fertilizer with N, P2O5, K2O≥30%（A,B,C,D and E treatments） promoted sugarcane tillering, appropriately applying with organic matter in which（B and D treatments） had better effect. At the same time, the smut and borer pest also got better control in sugarcane seedling. The pesticides fertilizer containing organic matter had a better indirect effect on smut, and which containing chlorantraniliprole and thiamethoxam could prevent and control borers in best. In addition, the application of pesticides fertilizers containing organic matter（B,D and F treatments） could not only promote the elongation of sugarcane stem in advance, but also promote rapidly growth for plants, and to form more millable canes, then increase their yield per unit area. Under the premise of the same cost of fertilizer, the agricultural increased of sugarcane treated with different fertilizer reached by 2118.62-9199.31 yuan/hm~2, among which D treatment had the highest value added.【Conclusion】The application of organic-inorganic compound pesticides fertilizer during the inter-tillage period have positive effect on the control of diseases and insect pests of sugarcane seedlings, and the control effect is closely related to the content of medicinal ingredients and nutrient ratio of fertilizer. The tillering of sugarcane can be effectively promoted by applying medicated fertilizer, which could also effectively improve the effect for sugarcane production, and two kinds of which the component of 35% N, P2O5, K2O content（17-7-11）,0.10% insecticide, 0.10% thiamethoxam, 3% organic matter or 30% N, P2O5, K2O content（13-6-11）,30 mL Durui, 0.10% thiamethoxam, 15% organic matter are more beneficial to increase sugarcane production efficiency, thus it is the best for sugarcane cultivation in Guangxi. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to explore effects of different shading models on tea garden habitat and the quality of summer tea, to find out the suitable shading models for tea trees, so as to provide theoretical basis for the production of high-quality summer tea in Guangxi. 【Method】In summer of 2020, Jinxuan was used as the test variety, and black shading net was used as the shading material and 4 shading treatments T1（60% shading+1. 3 m shed covering）, T2（60% shading+1. 8 m shed covering）, T3（75% shading+1. 3 m shed covering） and T4（75% shading+1. 8 m shed covering） were set, with no shading as the control（CK）. Cultivation experiments of different shading models were carried out in tea garden, and the tea garden habitat index（illumination intensity, air temperature, air relative humidity and soil temperature） and the quality ingredients index of tea（chlorophyll, tea polyphenol, amino acid, vitamin C, water extractive, caffeine） were analyzed. 【Result】Different shading models could effectively reduce the illumination intensity, air temperature and soil temperature, increase the air relative humidity, improve the habitat of tea garden and improve the quality of summer tea. Effect of treatments T3 and T4 on improving tea garden habitat were better than those of treatments T1 and T2. After 25 days of shed covering, the total chlorophyll contents of summer tea were T3>T4>T2>T1, which significantly increased by 79. 66%, 56. 13%, 28. 24% and 17. 31%（P<0. 05, the same as below） compared with CK, respectively. The tea polyphenols contents were T4T3>T2>T1, which significantly increased by 14. 67%, 10. 67%, 5. 78% and 4. 00% compared with CK, respectively. The proportion of essential amino acids in amino acid components were T4>T2>T1>T3, which were 2. 85%, 2. 75%, 1. 77% and 1. 59% higher than that of CK, respectively. The ratio of tea polyphenol to amino acid were T4T4>T1>T3, which significantly increased by 13. 74%, 12. 73%, 10. 01% and 8. 31% compared with CK, respectively. The water extract contents were T4>T2>T3>T1. In comparison with CK, the water extract contents of T4 and T2 treatments significantly increased by 65. 53% and 16. 00%, T3 treatment decreased by 3. 92% but did not reach significant difference（P>0. 05, the same below）, while T1 treatment decreased significantly by 5. 28%. And there were no significant differences in caffeine contents between shading treatments and CK. 【Conclusion】The model of 75% shading+1. 8 m shed covering can significantly improve the habitat of tea garden and improve the integrating quality of summer tea. This model is recommended as a supporting technology for the high quality summer tea production to be extended in Guangxi. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to analyze the blocking effect of different blocking inhibitors on cadmium（Cd） and arsenic（As） in heavy metal contaminated rice fields and their effect on the yield of different varieties of rice, in order to provide a reference basis for the screening of rice varieties with low accumulation of heavy metals and the production of safe rice.【Method】Using the field test method, 16 Guangxi main planted late rice varieties were planted in the soil with high risk of heavy metal pollution, and two kinds of inhibitors, selenium-inhibitor and silicon-inhibitor, were tested for heavy metal control. The Cd and As contents in the rice samples of each variety were measured, and the blocking effects of different blocking agents on Cd and As and the yields of different varieties of rice were compared and analyzed.【Result】The Cd and As contents of the 16 rice varieties differed significantly, among which five varieties, namely, Shenliangyou 8386,Guang 8 you jinzhan, Fengtianyou 553,Guixiang 12 and Guang 8 you 169,had lower Cd enrichment ability, and four varieties, namely, Yexiangyou 703,Hengfengyou 666,Guang 8 you jinzhan and Yexiangyou lisi, had lower As enrichment ability. The yield of each rice variety sprayed with selenium-inhibitor and silicon-inhibitor was significantly improved, with an average yield increase of 6.5% and 5.4%,respectively, compared with the control（CK）. After spraying selenium-inhibitor and silica-inhibitor, the Cd content of rice of 16 rice varieties was lower than 0.200 mg/kg, and the average content was 0.119 and 0.125 mg/kg, respectively, which were significantly lower than that of CK（0.189 mg/kg）. The average contents were 0.119 and 0.125 mg/kg, which were 37.03% and 33.33% lower than those of CK（P<0.05）. The mean As contents of rice were 0.197 and 0.185 mg/kg, respectively, which were not significantly different（P>0.05） compared to CK（0.186 mg/kg）.【Conclusion】The test results show that there are significant differences in the adsorption capacity of different late rice varieties of rice for heavy metals Cd and As.Foliar spraying of selenium and silicon inhibitors in Cd-contaminated rice fields can significantly improve rice yield and also significantly reduce the Cd content of rice, but has no significant effect on reducing the As content of rice. The five low Cd accumulation rice varieties, namely Shenliangyou 8386,Guang 8 you jinzhan, Fengtianyou 553,Guixiang 12 and Guang 8 you 169,can be promoted and planted in local Cd contaminated rice fields, and the four low As accumulation rice varieties, namely Yexiangyou 703,Hengfengyou 666,Guang 8 you jinzhan and Yexiangyou lisi, can be promoted and planted in local As contaminated rice fields. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to provide a scientific reference for drought prevention and disaster avoidance of sugarcane production, the temporal and spatial characteristics of sugarcane drought in different growth period in Guangxi were analyzed in recent 60 years. [Method] Firstly the development period of sugarcane, soil data and meteorological data from 1961 to 2020 in Guangxi were collected for the study. Under major concentration for effectiveness of precipitation and dynamic change characteristics of crop coeffi cient, the temporal and spatial variation characteristics of sugarcane drought in growth period were analyzed by linear and periodic analysis method and spatial station technology basing on crop water deficit index. [Result] The interannual drought incidence was the highest in maturity stage, followed by stem elongation stage, and the least in seedling stage and in tillering stage. Light and medium drought are mainly in seedling stage, tillering stage and stem elongation stage. Heavy and extreme drought are mainly in maturity stage. During the study period, there was no obvious linear change trend in the interannual drought incidence at all levels and with a periodic change on the scale of 2 - 5 years. The years of occurrence were mainly in 1970s and 1980s. In terms of spatial distribution, in the seedling stage light drought and medium drought were mainly distributed in the west and south of Guangxi. The occurrence frequency of light drought was less than 40%. The occurrence frequency of medium drought was less than 30 % . The occurrence frequency of heavy drought was less than 10 % and its distribution range was small. In the tillering stage, light drought occurred in all over the region. The occurrence frequency in the southwest of Guangxi was the highest, generally between 21 % and 40%. Medium drought was mainly distributed in the southwest of Guangxi and the occurrence frequency was less than 10% . The occurrence frequency of severe drought was less than 10 % and its distribution range was small. In the stem elongation stage, mild drought was distributed in the whole region with the occurrence frequency between 11 % and 70%. Medium drought was mainly distributed in the northeast of Guangxi with the occurrence frequency between 21 % and 60%. Heavy drought was mainly distributed in the northeast of Guangxi with the occurrence frequency less than 20%. There was no extreme drought in the seedling stage, tillering stage and stem elongation stage during the study period. In the maturity stage, light drought, medium drought, heavy drought and extreme drought were distributed in whole study region. The occurrence frequency of light drought was less than 30%. The occurrence frequency of medium drought and severe drought were less than 40%. The occurrence frequency of extreme drought was the most and its distribution was wide. The regions with high extreme drought occurrence frequency were mainly distributed in the center, south and east of Guangxi with the occurrence frequency between 31 % and 50%. [Conclusion] The light and medium drought are the main drought in the seedling stage, tillering stage and stem elongation stage of sugarcane in Guangxi during the sugarcane development. The heavy and extreme drought is the main drought in the maturity stage. In the maturity stage of sugarcane, the heavy and extreme drought occur once every 2 - 3 years in the center, south and east of Guangxi. The regions above should be get more attention for drought prevention and disaster avoidance. [ABSTRACT FROM AUTHOR]

[ Objective] The presnet paper aimed to understand flowering habits and pollen fenility of Pueraria lobata, and provide reference for improving the success rate of P. lobata cross pollination. [ Method] In Longzhou county, Guangxi, the bud development process of P. lobata inflorescences were observed every day after it was formed, marked the inflorescences that were about to open in the full bloom period, continuously observed the blooming process of the flowers and recorded the blooming time and weather conditions of the day. Triphenyl tetrazolium chloride (TIC) staining and 12 -KI staining were used to detect pollen activity of P. lobata at different bud stages ( half stretch period, presentation period, half period and blooming period), and the pollen with the strongest pollen viability was selected to carry out the pollen germination test according to the pollen viability measurement results. [ Result] In Longzhou county, Guangxi, P. lobata began to flower on October 16, reached the flowering stage on October 20, and the last flowering stage was from November 2 to November 5. The flowering time of P. lobata was mainly from 15: 10 to 15 :40 every day, and the flowering powder was affected by the weather conditions. Flowering was early when the weather was clear and the temperatute was high, while flowering was delayed when the temperature was low and cloudy and rainy. The pollen activity of P. lobata in different bud stages ( half stretch period, presentation period, half period and blooming period) detected by 12 -KI staining method were 82. l %,95. 3%,93. 5% and 92. 7%,respectively, which were significantly higher than TIC staining method ( P <0. 05, the same as below), and it was more suitable for the detection of the pollen activity of P. lobata. Most of the anthers of the P. lobata flower have already loosened their powder at the beginning of the presentation period, and the stigma was wrapped with large amount. The pollen vitality in the presentation period detected by 12-KI staining was the highest, reaching 95. 3%, which was significantly higher than the other three stages. The pollen quantity and pollen vitality remained at a high level from half stretch period to blooming period. In the medium containing 15 % sucrose, the germination rate of P. lobata pollen was the highest( 36. 23 % ), which was significantly higher than that of the other three sucrose concentrations. [ Conclusion]The 12 -KI staining method can quickly detect the pollen viability of P. lobata. The pollen viability of P. lobata was maintained at a high level from half period to blooming period, and the germination rate was the highest in the medium containing 15% sucrose in sunny weather. 12 -Kl staining method can be used to detect pueraria pollen viability rapidly. Pollen viability of P. lobata remained at a high level from bud opening stage to blooming stage, and pollen at a suitable period could be selected for cross-pollination according to weather conditions to improve the success rate of cross-pollination. [ABSTRACT FROM AUTHOR]

[ Objective] The present paper aimed to understand the spatial distribution characteristics of Bradysia difformis in oyster mushroom plantations, and analyze the sampling techniques of field investigation, so as to provide scientific basis for monitoring B. difformis and formulating control programs. [ Method] From March to May in 2020, a dense checkerboard sampling method was used to investigate oyster mushroom in Yuhui Edible Fungus farm, Santang town,Xingning district, Nanning, Guangxi, by counting the number of adults attached to the hyaline board suspended at the sample points, the flight altitude of the adults of B. difformis was determined. The spatial distribution pattern of B. difformis was determined by frequency distribution method, aggregation index method, Taylor's power function method and lwao linear regression analysis method, and the spatial distribution pattern of B. difformis adults in ostreatus planting canopy was determined. The error rate and fitness of different sampling methods were calculated by simulated sampling method. [ Result] In the ostreatus shed, the number of adults adhered to the armyworm plate at 10. 00 cm above the fungus pouch was the largest, which was significantly higher than other heights ( P < 0. 05) . The x2 values of negative binomial distribution in the three surveys were 23. 680, 31 . 410 and 21. 740, respectively, and there was no significant difference between the actual and theoretical frequency test results of negative binomial distribution ( P > 0. 05 ) . The x² values of negative binomial distribution in the three surveys were consistent with negative binomial distribution. The dispersal index ( C > l. 000 ), negative binomial distribution index(O < K < 8. 000 ),clustering index ( 1 > 0 ), clustering index ( m */ m> 1),and spatial distribution pattern of the adults were aggregation distribution. According to m • -m regression model and taylor power fitting model, the basic component of adult population distribution of B. in mushroom shed was individual group, which was attracted to each other, and the individual group belonged to aggregation distribution, which was consistent with the aggregation index method. Among the fitness comparison among different sampling methods the Z-shaped sampling method(5. 72% ) and the diagonal sampling method ( 5. 71% ) had the lowest error rate, and the theoretical sampling number model was N = ( t² / D² ) ( L 4741/ m + 0. 3824 ) . [ Conclusion] In the ostreatus planting shelter, the flight height of the adults of B. difformis was mainly concentrated at 10. 00 cm above the pouch, and the spatial distribution was dominated by a highly concentrated negative binomial distribution. The ideal sampling methods for field investigation of B. difformis were Z-shaped sampling method and diagonal sampling method. [ABSTRACT FROM AUTHOR]

[Objective] To clarify the pathogenic fungus of passion fruit stem rot and its biological characteristics, and to provide theoretical basis for its scientific prevention and control. [Method] This paper used the conventional isolation method of phytopathogenic fungi to isolate the stem-base rot pathogen of passion fruit collected from Guangxi under different ecological conditions. The morphological characteristics were observed and the pathogenic traits were determined. The rDNA-ITS sequences were analyzed to identify the pathogenic fungus causing passion fruit stem rot. [Result] The same morphological strain 32-WUB3 was isolated from typical pass ion fruit stem rot symptomatic plants collected from various pass ion fruit planting bases in Guangxi. After morphological, molecular biological and pathogenicity tests, Lasiodiplodia theobromae was identified as the causal agent of passion fruit stem rot. The mycelial growth rate of the pathogen was high at 28 and 30°C. The colony diameters were 8. 27 and 8. 31 cm, respectively, which were extremely significantly higher than those at other temperatures (P < 0. 01, the same as below). The fastest mycelial growth was under full light conditions, with a colony diameter of 8. 49 cm. Its diameter was extremely significantly higher than that of colonies in total darkness, and significantly higher than that of colonies in half light and half darkness (P < 0. 05, the same as below). The optimum pH for mycelial growth was 3. 00 - 8. 00. In this pH range, the mycelium grew faster and the colony diameter reached 8. 10 - 8. 44 cm. The difference between treatments was not significant( P > 0. 05), but there was a significant difference in colony diameter from pH 9. 00 to 11. 00 for each treatment. Mycelium grew the fastest in PDA medium. Its colony diameter reached 9. 00 cm, which was extremely significantly higher than the colony diameter of other media Mycelium grew the fastest when the car bon source was glucose. The colony diameter reached 7. 78 cm, which was significantly higher than the colony diameter of other carbon sources. The mycelium grew the fastest when the nitrogen source was yeast powder. The colony diameter was 7. 13 cm. The nitrogen sources as followed were peptone, DL-alanine, L-arginine and glycine. The differences among the treatments were not significant, but all were significantly higher than the colony diameter with beef paste, urea, ammonium dihydrogen phosphate, ammonium chloride, L-lysine and CK as the nitrogen source. The mycelial lethal conditions of this pathogenic mycelium were 52°C and 10 min of treatment [Conclusion] L. theobromae is the causal agent of stem rot of passion fruit The suitable temperature for mycelial growth of this pathogen was 28 - 30°C, pH was 3. 00 - 8. 00, lighting was full light, and the medium was PDA The carbon source was glucose, the nitrogen source was yeast powder, and the mycelial lethal condition was treatment at 52°C for 10 min. [ABSTRACT FROM AUTHOR]

[Objective J The present paper aimed to explore the diversity and distribution rules of soil microbial community of Annona squamosa Lour. after the infection of root rot, and provide a reference basis for the screening and identification with A. squamosa pathogens of root rot. [Method J Samples of A. squamosa rhizosphere soil infected with root rot (infected rhizosphere soil) and healthy rhizosphere soil (healthy rhizosphere soil) were collected. Using high throughput sequencing technology and designing primers of ITSl and 1154,sequenced the fungal ITSl and bacterial V3 + V4 regions, and analyzed the structure and diversity of the phylum, class, order, genus, and species level. [Result] After infection with root rot, the number of fungi in its rhizosphere soil increased greatly, and the number of bacteria decreased. The classification of fungi in rhizosphere soil belonged to 2 kingdoms,5 phylum, 16 classes,36 orders,48 families,81 genera and 96 species. The classification of fungi of healthy rhizome soil belonged to 3 kingdoms, 7 phylum, 17 classes, 43 orders, 73 families, 113 genera and 160 species. The community richness and diversity index of the rhizosphere soil microorganisms decreased compared with healthy rhizosphere soil, along with fewer types of soil fungi, and uniform distribution of soil fungi. After 4 days, medium and radioactive strains were formed on PDA medium; the strains formed medium, radioactive gas bacteria; colony diameter 4. 5 cm, bacteria flat spread, full edge, round, white, gas bacteria velvet. Rhizosphere soil pathogen strains were amplified using trial primers, the results showed that the displayed strain ITS sequence and Fusarium acutatum (accession number: AY569567. 1) had 99. 45% homology, indicating the amplified ITS segment sequence (RRD- 1) was parts of F. acutatum sequences. [Conclusion] The community richness and diversity index of root soil microorganisms decreased greatly compared with that of healthy soil, and the types of soil fungi in pathogenic plants were less, and the uniformity increased. After pathogen morphological observation and biological comparison, the pathogen that caused root rot of A. squanwsa in Guangxi might be F. acutatum. [ABSTRACT FROM AUTHOR]