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[Objective] The present paper aimed to provide theoretical reference for the breeding of rapeseed varieties with high photosynthetic efficiency, QTL mapping of chlorophyll content (CC) and candidate genes within QTL confidence interval were identified. [Method] The chlorophyll content of KN double-haploid (KN DH) population containing 348 lines was investigated under two ecological environments. Based on the KN high-density genetic linkage map and the phenotype data for chlorophyll content, QTLs for chlorophyll content and candidate genes within QTL confidence interval were identified. [Result] Thirty- four QTLs for chlorophyll content were detected. According to QTL integration, 31 consensus QTLs were identified, among which three QTLs were detected in at least two plant environments. Two QTLs cqCC.A7-3 and cqCC.A7-5 were identified in winter and spring ecological environments, which were regarded as the environmental stably express QTLs. In particular, one major QTL for chlorophyll content was identified in winter ecological environment and regarded as one major QTL of the winter environmental stably. Sixty-six candidate genes within QTL confidence intervals were detected, and their function involved in chlorophyll biosynthesis and degradation, light signal and electron transport, etc, such as BnaA01g14880D (ACD1-LIKE), BnaA07g29990D (AtWSCP), BnaA01g22670D (LHCA3), BnaC08g43200D (PSAO), BnaC06g28800D (FTSH1), BnaC08g19460D (SAUL1), etc. [Conclusion] QTLs for chlorophyll content and potential candidate genes in rapeseed can provide gene resources and theoretical basis for fining mapping, genetic analysis and high photosynthetic efficiency of chlorophyll content. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to screen antagonistic bacterial strains of mango anthracnose, so as to lay a theoretical basis for biological control of mango anthracnose and provide strain resources.【Method】Colletotrichum asianum,the dominant species of mango anthrax, was used as the target, and 37 strains of Bacillus spp. obtained in previous studies were used for confrontation culture and in vitro inoculation test. The antagonistic strains with obvious control effect were selected and their antagonistic mechanism was analyzed.【Result】The results of confrontation test showed that the inhibitory rates of 10 antagonists against C. asianum were more than 78.00%,among which the inhibitory rates of M12-5,L18-7,L19-3,L17-3 and M11-6 reached 96.24%,94.94%,94.10%,91.25% and 90.00%,respectively. Eight strains of Bacillus spp. with obvious control effect were obtained by in vitro inoculation, among which strains L18-7 and L16-4 had the best control effect, with the average control efficiency of 83.00% and 79.16%,respectively. There was no significant difference between them(P＞0.05). Combined with morphological characteristics, 16S rDNA sequence and phylogenetic tree analysis based on rpoB gene sequence, strain L18-7 could be identified as B. velezensis. Strain L18-7 could produce extracellular protease and cellulase, and could produce active antagonists in five media including Luria-bertani(LB),beef peptone(NA) and potato glucose agar(PDA) etc. Strain L18-7 carried genes related to antibiotic production such as mycB,ituA,yndJ,fenB and bacA.【Conclusion】The antagonistic bacterium strain L18-7 of mango anthracnose was identified as B. velezensis. The strain grew well on LB medium at 28 ℃.It could produce antagonistic substances, extracellular protease and cellulase and carried related genes of antibiotics such as mycB,ituA,yndJ,fenB and bacA. It can be used as germplasm resources for biocontrol bacteria. [ABSTRACT FROM AUTHOR]

【Objective】The aim of this paper is to analyze the genetic diversity of faba bean germplasm resources by transcriptome sequencing and SSR molecular marker technology， and to explore the molecular markers associated with bruchid resistance traits in faba bean. So as to provide a reference basis for the conservation and utilization of bruchid resistance germplasm resources and the selection and breeding of new varieties. 【Method】Transcriptome sequencing of two faba bean varieties Y134 and Y078 was performed by PacBio triple full-length sequencing technology combined with RNA-seq.. Based on this， all Unigene of the transcriptome were searched for Unigene containing SSR core motifs using the software MISA， and primers were designed in batch using Primer 3.0. One hundred pairs of SSR primers were randomly selected for genetic diversity analysis of 80 faba bean germplasm resources. 【Result】Fifty of the 100 SSR primer pairs amplified products， with an effective amplification rate of 50%; 32 of the primer pairs showed polymorphism. They accounted for 64% of the effective products and 32% of the total primers. Among the 32 polymorphic primer pairs， each pair amplified 2 to 21 alleles with an average of 6.875; The polymorphic information content （PIC） values ranged from 0.25 to 0.82 with an average of 0.58; The Shannon’s information index varied from 0.34 to 2.31 with an average of 1.21. Based on UPGMA cluster analysis， the 80 faba bean materials were classified into two major groups at a similarity coefficient of 0.70. The first category contained 65 germplasm， including 62 highly resistant （HR） varieties， 2 highly sensitive （HS） varieties and 1 sensitive （S） variety; The second category contained 15 germplasm， 7 highly susceptible （HS） varieties， 2 sensitive （S） varieties， 5 highly resistant （HR） varieties and 1 moderately resistant （MR） variety. 【Conclusion】The SSR markers developed were used to analyze the genetic diversity of 80 faba bean germplasm resources and to lay the theoretical foundation for research on the identification of faba bean germplasm resources resistant to bean weevil and molecular marker-assisted selection for breeding. [ABSTRACT FROM AUTHOR]

【Objective】In recent years, the wheat in Sichuan province is threatened by Fusarium head blight（FHB） or scab as changes in climate and farming practices.The present paper aimed to obtain the genetic resources of resistance to FHB in Sichuan wheat varieties, and breed wheat for resistance to FHB.【Method】In the research, 156 wheat varieties or high-generation lines with moderate resistance to FHB were identified by soil surface inoculation.The resistance gene composition of these wheat varieties was detected by molecular markers of FHB resistance genes Fhb1, Fhb2, Fhb4 and Fhb5, as well as some stripe rust resistance genes and powdery mildew resistance gene.【Result】Through molecular detection of disease resistance genes, it was found that 20yin979 and 20jian2506-10 contained the FHB resistance genes Fhb1 and Fhb5, respectively. For stripe rust resistance genes detection, 4, 63, 9, 3 and 14 wheat materials containing the Yr5, Yr15, Yr18, Yr26 and YrAs2388 were detected, respectively. And the distribution frequencies of rust resistance genes Yr5, Yr15, Yr18, Yr26 and YrAs2388 were 2.56%, 40.38%, 5.77%, 1.92% and 8.97%, respectively. A total of 44 materials containing the powdery mildew resistance gene Pm21 were detected, and the distribution frequency in the tested materials was 28.21%.The distribution of resistance genes showed that 122 materials contained at least one resistance gene.Among them, 6 materials contained both strip rust and powdery mildew resistance genes, 9 materials contained 2 strip rust resistance genes, and the remaining 106 materials contained only 1 resistance gene. The only material 20jian2506-10 contained three resistance genes of FHB, stripe rust and powdery mildew.【Conclusion】These results indicate that Sichuan wheat lacks FHB resistance resources with explicit genetic background.Therefore, Sichuan wheat FHB resistance breeding should strengthen the introduction of known resistance resources while making full use of the local FHB resistance resources. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to establish the evaluation method of heat resistance of leek resources, select heat-resistant leek varieties, and solve the problem of shortage of heat-resistant leek varieties in Guangxi.【Method】Taking three kinds of local germplasm resources of leek（marked as A, B, C, D, E, F, G, H and I, respectively） of Guangxi large leaf leek, wide leaf leek and thin leaf leek collected from the general survey of germplasm resources by Ministry of Agricultural and Rural Affairs（2015-2020） as test materials, the changes of the maximum growth length of leek leaves, the newly increased aboveground biomass and the contents of chlorophyll, proline and malondialdehyde after 7 days of high temperature stress at 38 ℃ were measured. Combined with field natural high temperature stress（field group） actual heat resistance performance, the heat resistance of different types of leek was comprehensively evaluated by multivariate statistical analysis（variance analysis, principal component analysis, membership function method and cluster analysis） methods.【Result】Under high temperature stress of 38 ℃,the maximum leaf growth length and the newly increased biomass of 9 leek germplasms of the three types in Guangxi were extremely significantly decreased compared with their proper temperature control（CK）（P＜0.01）.The largest decline was the type of large-leaved leek, and the smallest decline was the type of fine-leaved leek. Chlorophyll content decreased to different degrees, of which the largest decline was the type of leek with large leaves, and the smallest decline was the type of leek with thin leaves. The content of MDA increased to different degrees, of which the largest increase was the type of leek with large leaves, and the smallest increase was the type of leek with thin leaves. Pro content increased to different degrees, of which, the largest increase was the type of leek with thin leaves, and the smallest increase was the type of leek with large leaves. The comprehensive evaluation results showed that the heat tolerance of the three kinds of local leek germplasm resources of leek in Guangxi was ranked as follows: leek with thin leaves＞leek with broad leaves＞leek with large leaves. Germplasm G（No.2016451159） and Germplasm I（No.2016453604） had the strongest heat tolerance among the nine leek germplasms, and Germplasm C（No.SC20191200） had the weakest heat tolerance.【Conclusion】The maximum growth length of leaves, new aboveground biomass and the content of chlorophyll, Pro and MDA contents were used as the evaluation indicators of heat tolerance of leek germplasm resources, the heat tolerance of three kinds of leek germplasm resources in Guangxi can be ranked as follows: Leek with thin leaves＞ leek with broad leaves＞leek with large leaves by variance analysis, principal component analysis, membership function and cluster analysis. Germplasm G and germplasm I were determined to be more heat-resistant, while germplasm C was the least heat-resistant. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to explore the effect of light intensity on PSII activity of different Schisandrae chinensis.【Method】The leaves of S. chinensis resources ‘Zaohong’ and ‘Tieling-0601’ were treated with 0, 600, 1200 and 1800 μmol/（m²·s） light.【Result】Strong light damaged the photosynthetic machinery easily. High light inhibited the activity of the PSII donor and recipient sides, which hindered electron transport, accumulated a significant amount of ROS and MDA, and caused membrane peroxidation. K point appeared in both ‘Zaohong’ and ‘Tieling-0601’ under strong light, and relative variable fluorescence intensity at the K-step（VK） and relative variable fluorescence intensity at the J-step（VJ） increased with the increase of light intensity, and VJ point was the highest under a light intensity of 1800 μmol/（m²·s）. VJ of ‘Tieling-0601’was higher than that of ‘Zaohong’in all treatments. Compared with the control, the electron transport efficiency（φO）, quantum yield of electron transport(φEo), number of reaction centers（RC/ABS）, quantum yield of electron transport per unit area（ETO/CSO） and captured light energy per unit area（TRO/CSO） of PSII chlorophyll antenna decreased under strong light treatment. The quantum ratio for heat dissipation(φDo), the light energy absorbed per unit area（ABS/CSO） and the heat dissipation per unit area（DIO/CSO） increased. With the increase of light intensity, the accumulation of malondialdehyde（MDA） in both‘Zaohong’and ‘Tieling-0601’increased, and the MDA accumulation in‘Tieling-0601’was higher than that in ‘Zaohong’. There was a significant difference in anthocyanin accumulation of leaves between ‘Zaohong’ and ‘Tieling-0601’, and ‘Zaohong’ was 3 times higher than ‘Tieling-0601’. 【Conclusion】Different germplasm resources of S. chinensis had different responses to high light, and PSII electron transporter activity was stronger under ‘Zaohong’ high light stress, and the ability to resist high light was stronger. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to enrich the salt tolerance gene resources of Arundo donax and provide help for molecular breeding.【Method】The ‘LZ＿ No.1’ A. donax with consistent growth were treated with 0, 50, 100, 150 and 200 mmol/L NaCl salt solutions for 36 hours, and each treatment consisted of 3 replicates. Stems from the same site were collected for transcriptome sequencing and bioinformatics analysis. 5 differentially expressed genes were randomly selected for qRT-PCR to verify the expression pattern.【Result】De novo assembly was performed on the sequencing data after quality control, and a total of 971 309 transcripts were generated, with a GC content of 47.14%, and the N50 of Unigene contigs reached 1126 bp, indicating a good assembly effect. The 7305 differentially expressed genes identified were subjected to GO and KEGG enrichment analysis, and the results showed that stems of ‘LZ＿ No.1’ responded to salt stress mainly through nitrogen metabolism, ion transport, terpenoid synthesis, zeatin synthesis, linoleic acid metabolism, salicylic acid biosynthesis and osmotic regulator substances. The differentially expressed genes were screened, and a total of 104 core differentially expressed genes were obtained, of which 88315＿c0＿g1＿i1, 1200＿c2＿g1＿i1, 13330＿c0＿g1＿i8, 46571＿c0＿g1＿i7, 3434＿c0＿g1＿i11 and 713＿c0＿g1＿i32 genes with large differential multiples may play an important role in the response to the salt. The identification of transcription factors indicated that AP2/ERF-ERF family, MYB-related family and WRKY family were the most important gene families in salt response, and most of these three transcription factor family members were up-regulated in response to salt stress, indicating that they mainly worked by increasing the expression of genes. The detection results of the five differentially expressed genes by qRT-PCR were basically consistent with the RNA-seq results, which also demonstrated the reliability of the transcriptome data.【Conclusion】The study identifies important genes and transcription factors of A.donax in response to salt stress, which can lay a solid foundation for its future genetic improvement work. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to clone the transcription factor gene BoWRKY10 of kale (Brassica oleracea var. acephala DC) and study the drought tolerance regulation mechanism of the gene.【Method】The gene BoWRKY10 was amplified from the cDNA of kale leaves by RT-PCR and transferred into tobacco. The expression pattern of the gene under drought stress was detected by real-time fluorescence quantitative PCR.【Result】The survival rate of transgenic lines were significantly higher than that of non-GMO wild types (WT) after 10 days of drought. There was no obvious difference between transgenic lines (OE3, OE4 and OE6) and WT in terms of germination rate in 1/2 MS culture medium, however, in medium containing 15% PEG4000, germination rate of OE3, OE4 and OE6 were 65.9%, 75.4% and 84.3%, respectively, while the one of WT was 22.5%. In medium containing 15% PEG4000, the root length of transgenic lines was significantly higher than that of WT after 14 days. Meanwhile, ABA content, proline content, soluble sugar content, POD, CAT and SOD activities were higher, while MDA and H2O2 content were significantly lower than those of WT. The expression level of drought responsive gene in OE3, like NtSOD, NtAPX, NtCAT, NtGST, NtSPSA and NtNCED1, was significantly higher than that of WT.【Conclusion】Expression of BoWRKY10 from kale remarkably improved the tolerance of tobacco to drought stresses in which the pleiotropy could be beneficial gene resource of gene engineering about resistance to stress in plant. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to illustrate the potential utilization of the gene of resistance to bacterial blight and bacterial leaf streak, so as to provide reference for enriching genetic base of rice breeding.【Method】A total of 102 rice cultivars were used to screen by molecular marker for the gene xa5, which mediated the resistance for both for resistance to bacterial blight and bacterial leaf streak. The parents carrying xa5 gene and having good resistance to bacterial blight and bacterial leaf streak were used as resistant sources to cross with susceptible cultivated rice. Through pedigree method and molecular marker-assisted selection（MAS）, elite hybrid rice restorer lines were cultivated. The excellent restorer lines and sterile lines were combined with strong heterosis combinations to analyze the resistance and comprehensive agronomic traits of strong heterosis combinations.【Result】A total of 17 cultivars were screened, which carried xa5 gene from 102 rice germplasm resources（materials）. Among these cultivars, DP3, IRBB5, N36, N776, N777, N779 and P89 were well resistant to both bacterial blight and bacterial leaf streak. An elite line Y240-1 was selected from the cross between DP3 and 9311. Moreover, 998 R was crossed with elite individual Y240-1. With molecular MAS, an elite restorer line Yexiangzhan with xa5 was developed from the cross of 998 R and Y240-1, after multiple generations of selfing. Yexiangzhan was combined with sterile line Pingfeng A to produce an elite hybrid cultivar Pingfengyouxiangzhan, which showed higher resistance to bacterial blight and bacterial leaf streak in comparison with Pingfeng A. Pingfengyouxiangzhan was resistant to rice blast at moderate level and meet the 2nd class standard of fine quality issued by Ministry of Agriculture of China.【Conclusion】xa5 gene with resistance to bacterial blight and bacterial leaf streak of rice has partial dominant effect, which can be used for breeding improvement of hybrid rice; It is helpful to enrich and broaden the genetic basis of rice breeding by cultivating the superior hybrid combinations of rice with moderate resistance to bacterial blight and stripe disease with wild rice blood. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to analyze the genetic structure and variation of four Channa argus populations and three Albino Channa argus populations from different geographical locations. [Method] The DNA of 350 individuals in all populations was detected by 20 pairs of microsatellite primers. The polymorphism and genetic diversity of all microsatellite markers were analyzed. [Result] All 20 pairs of microsatellite primers detected a total of 356 alleles, the number of alleles (Na）ranged from 4 to 40, with an average of 17.8 alleles per locus. The observed heterozygosity (Ho) was between 0.000 to 0.791, and the mean value was 0.578.The expected heterozygosity (He) ranged from 0.31 to 0.91, with an average value of 0.732.The polymorphic information content (PIC) ranged from 0.271 to 0.901, and the average value was 0.700, the Na, Ho, He, and PIC of all the Channa argus populations were higher than those of the Albino Channa argus populations. The genetic differentiation index(FST) of the seven populations ranged from 0.07 876 to 0.40 303, and the genetic differentiation among all populations reached significant level (P<0.05). We divided four Channa argus populations and three Albino Channa argus populations into two groups by molecular variance analysis (AMOVA）, and found that the genetic variation of these groups was mainly from within individuals, accounting for 69.65% of the total genetic variation. The UPGMA hylogenetic tree showed that the Albino Channa argus and Channa argus were clustered into two independent clades. [Conclusion] In this study, the genetic diversity of four Channa argus populations was higher than three Albino Channa argus populations, the protection of genetic diversity of the three populations should be strengthened to avoid inbreeding. This study provides a theoretical basis for the protection and comprehensive utilization of genetic resources of Channa argus and Albino Channa argus. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to obtain the sequence and structural information of full-length transcripts of taro, and to identify the structural information of genes and transcripts related to starch biosynthesis, so as to provide a scientific basis for the subsequent elucidation of the molecular mechanism of starch biosynthesis and in-depth utilization of taro genetic resources. [Method] The full-length transcripts were sequenced from different tissues (leaves, petioles, corms, stolons and roots) at the age of 3 months using Lipu Taro 1 as the test material. The transcripts were annotated for analyzing their bioinformatics of structural information of AS,APA, lncRNA, TF and SSR sites. Genes and transcripts related to starch biosynthesis were obtained by KEGG annotation and analyzed for AS events and APA. [Result] A total of 38 043 full-length transcript sequences were obtained from three generations of full-length transcriptome sequencing of different tissues of taro. By comparing with the reference genome, 31 058 new transcripts were identified, of which 28 785 were functionally annotated. Structural analysis of the transcripts showed that 9360 AS events, 6436 genes with poly (A) sites, 25 081 SSR loci, 304 lncRNAs, 1911 fusion transcripts and 1608 TFs were identified.14 starch biosynthesis-related genes with 60 transcripts were obtained by KEGG annotation, and their AS events and APA analysis revealed that six genes had AS, containing five types of IR,A5 SS,A3 SS,ES and MEE, and 10 genes had poly (A) sites, of which seven genes had two or more poly (A) sites. [Conclusion] The sequence and structural information of the full-length transcripts of taro are obtained by full-length transcriptome sequencing analysis, and 14 genes and 60 transcripts related to taro starch biosynthesis are identified, which can be used as a scientific basis for the subsequent elucidation of the molecular mechanism of taro starch biosynthesis and in-depth utilization of taro genetic resources. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to look for a new way for collecting germplasm resources of wheat resistant to pre-harvest sprouting ( PHS). [Method ] The I, II and Ill batches of the seedlings of wheat grain left in the field from July 1 to July 31, August 1 to August 31 and September 1 to October 10 were collected, which were transplanted into observation nursery and in the middle of October, respectively.[Result] Through the observation of growth consistency, it was found that 93 % of the seedlings of wheat grain left in field in the I batch had the same direct agronomic traits as the wheat varieties planted in the previous crop, while the direct agronomic traits in the II and Ill batches were different. It was found that 94. 1 % of the I batch of the seedling of wheat grain left in the field were susceptible to sprouting, and only 3 . 6% and 2 . 3 % of the seedlings were moderately resistant and resistant, all of the II and Ill batches of the seedling of wheat grain left in the field showed higher resistance to PHS than the middle level, and the later the collection batch was, the higher the proportion of high resistance PHS single ear was; Through the analysis of growth environment factors of wheat seedling with resistance to PHS, then artificial distant hybridization was carried out, it was found that the seed setting rate of artificial distant hybridization between Aegilops tauschii Coss grown in the same field as the wheat seedlings and common wheat was 5 %, which fully proved the possibility of natural cross between A. tauschii and Triticum aestivum in the field; Detecting and analyzing the genomic DNA of ten wheat seedlings with the highest resistance to PHS, A. tauschii Coss, wheat varieties which were mainly cultivated near 10 years in the same field, the primers Xgdm33 and Xgwm52 not only detected a large number of DNA fragments from A. tauschii Coss, but also detected DNA fragments homologous to the previously planted wheat varieties, it was proved that the seedling of wheat grain left in the field was the offspring of natural hybridization between A. tauschii Coss growing in the field and previously planted wheat. [Conclusion] Using the method collecting the seedling of wheat grain left in the field, the wheat germ plasm resources with high resistance to PHSit could be obtained. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to search for molecular markers closely linked to the main agronomic traits of broad bean, and to explore the excellent allelic variation and germplasm of agronomic traits. [Method] 321 faba bean accessions were tested, and their initial pod height, initial pod nodes, pod nodes, reproductive nodes, effective grains, effective branches, effective pods, grains per pod, pod length, pod width, grain area, grain thickness, grain perimeter, grain length, grain width, plant height and 100-grain weight were investigated. 76 pairs of SSR markers with significant polymorphism were used for genotyping. Based on the analysis of population structure, the GLM and MLM models of Tassel 2. 1 software were used for correlation analysis of the above 17 agronomic traits. [Result] The variation coefficient of traits ranged from 10. 06 % to 51. 64 %, with an average value of 25. 07 %, indicating that the tested population had rich phenotypic diversity. A total of 389 polymorphic loci were detected by 76 markers in 321 faba beans, and the average number of alleles at each locus was 5. 12, with a variation range of 2 - 11. The PIC value ranged from 0. 1903 to 0. 7766, with an average of 0. 5156, indicating that the selected markers had high polymorphism. Structure analysis was performed using structure 2. 3. 4, and 321 samples into two subgroups, which showed that the population structure was relatively simple and thus it was beneficial to association analysis. Based on GLM model, eleven markers were detected associated with twelve agronomic traits (P < 0. 01), and the R2 of phenotypic variation by a single marker was 0. 0393 - 0. 1101. Based on the MLM model, eight markers were detected associated with eight agronomic traits were (P < 0. 01), and the R2 of phenotypic variation by a single marker was 0. 0291 - 0. 0897. Finally, based on the phenotypic effect of allelic variation in the extremely significant (P < 0. 01) association loci in the results of MIM model analysis, seven excellent allelic variations with the greatest synergistic potential on number of pods, number of grains per pod, effective pod number, effective grain number, plant height, grain thickness, grain length and grain perimeter, and seven excellent accessions were screened out. [Conclusion] Through association analysis, a number of molecular markers associated with agronomic traits of faba bean and excellent germplasm were mined, which laid a theoretical foundation for molecular marker-assisted selection breeding of faba bean and the selection of parental materials. [ABSTRACT FROM AUTHOR]

[Objective] The purpose of the present paper is to evaluate the genetic diversity of wild sugarcane germplasm resources, and to explore germplasm resources with breeding potential for directional genetic improvement of sugarcane stress resistance. [Method] In the study, the genetic diversity of 44 wild relatives materials divided into three levels according to inter-species, Saccharum spontaneum, S. arundinaceus were analyzed by ISSR molecular markers. [Result] Using 13 ISSR primers screened in 44 inter-species materials, a total of 261 DNA fragments were amplified, among which 259 ( 99%) were polymorphic; Interspecies polymorphism: Saccharum spontaneum > Saccharum arundinaceus > Erianthus fulvus > Miscanthus floridulus > Eulalia speciosa > Narenga porphyrocoma ; The genetic similarity coefficient among 44 inter-species materials varied from 0. 125 to 0. 710. The UPGMA clustering results of the 44 interspecies materials were basically consistent with the morphological classification. The 12 S. arundinaceus materials were clustered together in 0. 815; S. spontaneum and S . arundinaceus materials were close cluster together in 0. 75, Narenga; Miscanthus, Erianthus materials were closer than others clustering in 0. 73 with Saccharum materials. [Conclusion] The study showed that sugarcane wild germplasm resources had high genetic diversity, which could provide germplasm materials and theoretical basis for sugarcane genetic breeding. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to clarify the genetic background of strawberry hybrids and their parents. [Method] 10 ISSR primers were selected for genetic background analysis of strawberry hybrids and parents. We used the 10 ISSR primers to analyze 12 strawberry cultivars with complex genetic background. [Result] Between the parents, parents and their hybrids, parent and its tissue cultivating variants, the average genetic distances were 0. 3258,0. 2607 and 0. 1981 respectively, and the average genetic similarity coefficient were 0.6551, 0.7478 and 0.7672, respectively. The clustering analysis and multidimensional scaling analysis confirmed that strawberry cultivars had a relatively narrow genetic background between the tested materials, indicating that the selected 10 lSSR primers can be a powerful tool for strawberry genetic background analysis. [Conclusion] The genetic background analysis of strawberry will lay a foundation for germplasm innovation. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to explore the excellent genes of germplasm resources of Tiebt barley and provide high-quality materials for breeding. [Method] The genetic diversity, clustering and principal component analysis of 13 agronomic traits were carried out by using Excel and SPSS 21. 0 software with 140 germplasm resources from different areas of Tibet [Result] There was rich genetic diversity in barley resources in Tibet, and the coefficient of variation of panicle number per plant among 8 agronomic traits was 43.7%. Cluster analysis divided the 140 materials into five groups at the genetic distances of 6 level, of which 3 5 materials were in group I, 29 materials were in group II, 33 materials were in group Ill, 15 materials were in group N and 28 materials were in group V. The results of principal component analysis showed that when the 8 agronomic traits were simplified into 3 principal components, the cumulative contribution rate was 72. 587 %, and the contribution rate of panicle per plant was the highest, which was 0. 829. [Conclusion] The study provides a scientific basis for the protection and utilization of germplasm resources in Tibet. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to realize the genetic resources and genetic relationship of Henan Big-tail sheep, Small-tail Han sheep and Yuxi Fat-tail sheep.【Method】The genetic diversity of mitochondrion（ mt DNA） D-loop and genetic differentiation of Henan sheep breeds were analyzed in the present study.【Result】50 haplotypes were identified in total,23 in Small-tail Han sheep,25 in Yuxi Fattail sheep, and three in Henan Big-tail sheep. The haplotype diversity were 0. 975,0. 936 and 0. 667,the nucleotide diversity were 0. 0230,0. 0129 and 0. 0270 in Small-tail Han sheep, Yuxi Fat-tail sheep and Henan Big-tail sheep, showing abundant genetic diversity of D-loop sequence in Small-tail Han and Yuxi Fat-tail sheep. The phylogenetic tree gathered these three sheep breeds into three lineages（ A-C）,the three breeds exsisted in all three lineages. The genetic distance of Small-tail Han sheep and Yuxi Fat-tail sheep was the farest（ 0. 0303）. The genetic distance of Henan Big-tail sheep and Small-tail sheep was the nearest（ 0. 0260）. The neutrality test demonstrated that Small-tail Han sheep and Yuxi Fat-tail sheep maintained equilibrium（ P >0. 1）,while Henan Big-tail sheep undergone bottleneck effect（ P <0. 05） in recent decades. The network result showed three maternal origins of Henan sheep breeds. 【Conclusion 】Three Henan sheep breeds showed high genetic diversity. The genetic distances reach the standard of subspecies difference. Small-tail sheep was farthest to Yuxi Fat-tail sheep and nearest to Henan Big-tail sheep. Henan sheep breeds have three maternal origins. The study will provide theoretical basis for the study of genetic relationship and population history, and the protection of genetic resources for Henan sheep breeds. [ABSTRACT FROM AUTHOR]

[Objective] The pmpose of this paper is to obtain the basis information of Potyviruses distribution and investigate Potyvirus species between resistant and susceptible tobacco varieties to PVY in field in Yunnan Province. [Method] During 2017 to 2019,497 tobacco samples with typical Potyviral symptoms were collected in 5 cities, 13 tobacco planting counties (districts) in Yunnan Province and the types of Potyviruses on the main susceptible and resistant varieties to PVY were analyzed. [Result] The results showed that 85. 1 % of 497 tobacco samples were infected by Potyviruses in total. Furthermore, the detection rates of TVBMV, ChiVMV, PVY, and TEV were 58. 8 %, 23. 3 %, 14. 9% and 7. 8% respectively. In terms of the rank of detection rates of Potyviruses in the PVY susceptible varieties, the detection rate of TVBMV was the highest in Qujing,and for ChiVMV was in Yuxi,and for PVY was in Zhaotong. The PVY detection rate on the PVY-resistance varieties was 5. 8% around and much lower than that of susceptible varieties (22. 5%). PVY resistant varieties showed a good crop protection for the PVY disease. [ Conclusion] The analysis showed that the resistant varieties which contained va locus dramatically reduced the yield loss in the high incidence areas of PVY, and the increase of TVBMV detection rate in PVY resistant varieties was due to the decrease of PVY incidence rate, and the resistant varieties did not increase the harm of TVBMV and ChiVMV. However, the single va resistant locus had no effect on the TVBMV and ChiVMV. Therefore, the investigation of Potyviruses species should be carried out before introduce va-containing varieties into the area. TVBMV and ChiVMV had been the emerging disease in Yunnan's main tobacco growing areas, resulting in low yield and quality loss. It is necessary to establish new method to detect these causal agents and to screen moreresistant germplasm resources for TVBMV and ChiVMV. [ABSTRACT FROM AUTHOR]

[Objective] In order to provide a bas is for the development and utilization of excellent germplasm resources of wild kiwifruit in Yunnan Province, the sensory traits, morphological indicators, nutrient contents and nutritional quality of excellent germplasm resources of wild kiwifruit were comprehensively comparative analysis in this paper. [Method] The selection criteria were established with the objectives of large fruits, hairless or less hairy, and high quality, and the 'LB-2', 'LBBH-1 ' and 'XSD-1' were selected through the evaluation of sensory and morphological indexes, and their total sugar, total acid, vitamin C and soluble solids contents were determined and analyzed. According to the evaluation method of quality comprehensive index, the nutritional quality of 3 excellent germplasm resources was compared and analyzed with the cultivar 'Yunmi No. 1'. [Result] The order of fruit longitudinal diameter was 'XSD-1' > 'LB-2' > 'LBBH-1', and the difference between 'LB-2', 'LBBH-1' and 'Yunmi No. 1' were significant. The order of fruit transverse diameter was 'LBBH-1' > 'LB-2' > 'XSD-1', and the differences between the three excellent germplasm resources and 'Yunmi No. 1' reached a significant level. The order of fruit lateral diameter was 'LBBH-1' > 'LB-2' > 'XSD-1', the differences between the three excellent germplasm resources were not significant but reached a significant level with 'Yunmi No. 1'. The fruit weight per fruit was in the order of 'LBBH-1' > 'XSD 1' > 'LB-2', and the difference between the germplasm resources and 'Yunmi No. 1' reached a significant level. The total sugar and acid content of the germplasm resources were lower than that of 'Yunmi No. 1' in the order of 'LB-2 ' > 'LBBH-1' > 'XSD-1'. The order of sugar to acid ratio was 'XSD-1' > 'LBBH-1' > 'LB-2', with 'XSD-1' being the sweetest, 'LBBH-1 ' the second sweetest and ' LB-2 ' the least sweet in terms of flavor. The germplasm resources all had lower vitamin C content than 'Yunmi No. 1', and the highest soluble solids content was found in 'LB-2', while both 'LBBH-1 ' and 'XSD-1' had lower than the 'Yunmi No. 1'. The overall evaluation index was in the order of 'LB-2' > 'LBBH-1' > 'XSD-1' from high to low. [Conclusion] The order of comprehensive nutritional quality from high to low was 'LB-2' > 'LBBH-1' > 'XSD-1', and the three excellent resources of wild kiwifruit had uniform fruit size, neat and beautiful fruit shape, good flavor, fine texture, and rich juice, which had great development and utilization value. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to broaden the genetic background of Brassica napus and explore the application value of ge netic resources of new type B napus in breeding. [Method] 54 new-type B. napus lines were created by using the method of interspecific cross between Tibet mpeseed (B. juncea) local variety and Tibet B. napus Zangyou 5. [ Result] (i) The growth period of 54 new type B. napus lines ranged from 102 to 125 days, with an average of 119 days, showing the traits of early maturity. (ii) The comparison of yield traits showed that the 1000-grain weight of 54 new type B. napus lines ranged from 2.74 to 5. 42 g, of which 36 lines exceed their parents, and the 1000-grain weight of three lines XZ6, XZ21 and XZA2 was more than 5 g; the grain weight per plant of 54 new type B. napus lines ranged from 12.21 to 55.29 g, of which 12 lines exceed their parents, and the grain weight per plant of three lines XZ46, XZ47, XZ49 exceed 50 95. E, with higher yield potential. (iii) The comparison of quality characters showed that the oil content of the tested lines was 34.63% -51. 50%, with an average of 44.79%. Among them, the oil content of 8 lines exceeded 50%; the oleic acid content was 23.44% -69.52%, with an average of 51.54%; 13 lines exceeded the control. The line XZ11 not only had high oil content, but also had more than 65% oleic acid content. If its progenies were selected for oleic acid, it would be possible to breed lines with higher oil acid content. [Conclusion] The results indicated that there were more excellent characters in the new-type B. napus lines than that in the B. napus parents; the growth period was shorter, the yield traits were better, the oil content was higher, the oleic acid content above 65%. The new-type B. napus lines will be provided for breeding of B. na pus as new germplasm resources. [ABSTRACT FROM AUTHOR]