芦竹茎对盐胁迫响应的转录组分析.

【Objective】The present paper aimed to enrich the salt tolerance gene resources of Arundo donax and provide help for molecular breeding.【Method】The ‘LZ＿ No.1’ A. donax with consistent growth were treated with 0, 50, 100, 150 and 200 mmol/L NaCl salt solutions for 36 hours, and each treatment consisted of 3 replicates. Stems from the same site were collected for transcriptome sequencing and bioinformatics analysis. 5 differentially expressed genes were randomly selected for qRT-PCR to verify the expression pattern.【Result】De novo assembly was performed on the sequencing data after quality control, and a total of 971 309 transcripts were generated, with a GC content of 47.14%, and the N50 of Unigene contigs reached 1126 bp, indicating a good assembly effect. The 7305 differentially expressed genes identified were subjected to GO and KEGG enrichment analysis, and the results showed that stems of ‘LZ＿ No.1’ responded to salt stress mainly through nitrogen metabolism, ion transport, terpenoid synthesis, zeatin synthesis, linoleic acid metabolism, salicylic acid biosynthesis and osmotic regulator substances. The differentially expressed genes were screened, and a total of 104 core differentially expressed genes were obtained, of which 88315＿c0＿g1＿i1, 1200＿c2＿g1＿i1, 13330＿c0＿g1＿i8, 46571＿c0＿g1＿i7, 3434＿c0＿g1＿i11 and 713＿c0＿g1＿i32 genes with large differential multiples may play an important role in the response to the salt. The identification of transcription factors indicated that AP2/ERF-ERF family, MYB-related family and WRKY family were the most important gene families in salt response, and most of these three transcription factor family members were up-regulated in response to salt stress, indicating that they mainly worked by increasing the expression of genes. The detection results of the five differentially expressed genes by qRT-PCR were basically consistent with the RNA-seq results, which also demonstrated the reliability of the transcriptome data.【Conclusion】The study identifies important genes and transcription factors of A.donax in response to salt stress, which can lay a solid foundation for its future genetic improvement work. [ABSTRACT FROM AUTHOR]