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Your search keyword '"Gopinath, Subash C. B."' showing total 11 results
Start Over Author "Gopinath, Subash C. B." Journal scientific reports
11 results on '"Gopinath, Subash C. B."'

11. 'Spotted Nanoflowers': Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture.

Author

Perumal V, Hashim U, Gopinath SC, Haarindraprasad R, Foo KL, Balakrishnan SR, and Poopalan P

Subjects
Biosensing Techniques, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Photoelectron Spectroscopy, Spectrometry, X-Ray Emission, X-Ray Diffraction, Gold chemistry, Nanostructures, Zinc Oxide chemistry
Abstract

Hybrid gold nanostructures seeded into nanotextured zinc oxide (ZnO) nanoflowers (NFs) were created for novel biosensing applications. The selected 'spotted NFs' had a 30-nm-thick gold nanoparticle (AuNP) layer, chosen from a range of AuNP thicknesses, sputtered onto the surface. The generated nanohybrids, characterized by morphological, physical and structural analyses, were uniformly AuNP-seeded onto the ZnO NFs with an average length of 2-3 μm. Selective capture of molecular probes onto the seeded AuNPs was evidence for the specific interaction with DNA from pathogenic Leptospirosis-causing strains via hybridization and mis-match analyses. The attained detection limit was 100 fM as determined via impedance spectroscopy. High levels of stability, reproducibility and regeneration of the sensor were obtained. Selective DNA immobilization and hybridization were confirmed by nitrogen and phosphorus peaks in an X-ray photoelectron spectroscopy analysis. The created nanostructure hybrids illuminate the mechanism of generating multiple-target, high-performance detection on a single NF platform, which opens a new avenue for array-based medical diagnostics.

Published
2015
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