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'Spotted Nanoflowers': Gold-seeded Zinc Oxide Nanohybrid for Selective Bio-capture.

Authors :
Perumal V
Hashim U
Gopinath SC
Haarindraprasad R
Foo KL
Balakrishnan SR
Poopalan P
Source :
Scientific reports [Sci Rep] 2015 Jul 16; Vol. 5, pp. 12231. Date of Electronic Publication: 2015 Jul 16.
Publication Year :
2015

Abstract

Hybrid gold nanostructures seeded into nanotextured zinc oxide (ZnO) nanoflowers (NFs) were created for novel biosensing applications. The selected 'spotted NFs' had a 30-nm-thick gold nanoparticle (AuNP) layer, chosen from a range of AuNP thicknesses, sputtered onto the surface. The generated nanohybrids, characterized by morphological, physical and structural analyses, were uniformly AuNP-seeded onto the ZnO NFs with an average length of 2-3 μm. Selective capture of molecular probes onto the seeded AuNPs was evidence for the specific interaction with DNA from pathogenic Leptospirosis-causing strains via hybridization and mis-match analyses. The attained detection limit was 100 fM as determined via impedance spectroscopy. High levels of stability, reproducibility and regeneration of the sensor were obtained. Selective DNA immobilization and hybridization were confirmed by nitrogen and phosphorus peaks in an X-ray photoelectron spectroscopy analysis. The created nanostructure hybrids illuminate the mechanism of generating multiple-target, high-performance detection on a single NF platform, which opens a new avenue for array-based medical diagnostics.

Details

Language :
English
ISSN :
2045-2322
Volume :
5
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
26178973
Full Text :
https://doi.org/10.1038/srep12231