542 results on '"Charlier"'
Search Results
2. Bioconversion of 4-hydroxyestradiol by extradiol ring-cleavage dioxygenases from Novosphingobium sp. PP1Y
- Author
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Mensitieri, Francesca, Bosso, Andrea, Dal Piaz, Fabrizio, Charlier, Bruno, Notomista, Eugenio, Izzo, Viviana, and Cafaro, Valeria
- Published
- 2023
- Full Text
- View/download PDF
3. Volcanic glass from the 1.8 ka Taupō eruption (New Zealand) detected in Antarctic ice at ~ 230 CE
- Author
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Stephen B. Piva, Simon J. Barker, Nels A. Iverson, V. Holly L. Winton, Nancy A. N. Bertler, Michael Sigl, Colin J. N. Wilson, Nelia W. Dunbar, Andrei V. Kurbatov, Lionel Carter, Bruce L. A. Charlier, and Rewi M. Newnham
- Subjects
Medicine ,Science - Abstract
Abstract Chemical anomalies in polar ice core records are frequently linked to volcanism; however, without the presence of (crypto)tephra particles, links to specific eruptions remain speculative. Correlating tephras yields estimates of eruption timing and potential source volcano, offers refinement of ice core chronologies, and provides insights into volcanic impacts. Here, we report on sparse rhyolitic glass shards detected in the Roosevelt Island Climate Evolution (RICE) ice core (West Antarctica), attributed to the 1.8 ka Taupō eruption (New Zealand)—one of the largest and most energetic Holocene eruptions globally. Six shards of a distinctive geochemical composition, identical within analytical uncertainties to proximal Taupō glass, are accompanied by a single shard indistinguishable from glass of the ~25.5 ka Ōruanui supereruption, also from Taupō volcano. This double fingerprint uniquely identifies the source volcano and helps link the shards to the climactic phase of the Taupō eruption. The englacial Taupō-derived glass shards coincide with a particle spike and conductivity anomaly at 278.84 m core depth, along with trachytic glass from a local Antarctic eruption of Mt. Melbourne. The assessed age of the sampled ice is 230 ± 19 CE (95% confidence), confirming that the published radiocarbon wiggle-match date of 232 ± 10 CE (2 SD) for the Taupō eruption is robust.
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- 2023
- Full Text
- View/download PDF
4. HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
- Author
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Kolesnikova, Olga, Zachayus, Amélie, Pichard, Simon, Osz, Judit, Rochel, Natacha, Rossolillo, Paola, Kolb-Cheynel, Isabelle, Troffer-Charlier, Nathalie, Compe, Emmanuel, Bensaude, Olivier, Berger, Imre, and Poterszman, Arnaud
- Published
- 2022
- Full Text
- View/download PDF
5. Bioconversion of 4-hydroxyestradiol by extradiol ring-cleavage dioxygenases from Novosphingobium sp. PP1Y
- Author
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Francesca Mensitieri, Andrea Bosso, Fabrizio Dal Piaz, Bruno Charlier, Eugenio Notomista, Viviana Izzo, and Valeria Cafaro
- Subjects
Medicine ,Science - Abstract
Abstract Livestock breeding activities and pharmaceutical wastes lead to considerable accumulation of steroid hormones and estrogens in wastewaters. Here estrogens act as pro-cancerogenic agents and endocrine disruptors interfering with the sexual development of aquatic animals and having toxic effects in humans. Environmental bacteria play a vital role in estrogens degradation. Their wide reservoir of enzymes, such as ring cleavage dioxygenases (RCDs), can degrade the steroid nucleus, catalyzing the meta-cleavage of A, B or D steroid rings. In this work, 4 extra-diol ring cleavage dioxygenases (ERCDs), PP28735, PP26077, PP00124 and PP00193, were isolated from the marine sphingomonad Novosphingobium sp. PP1Y and characterized. Enzymes kinetic parameters were determined on different synthetic catecholic substrates. Then, the bioconversion of catechol estrogens was evaluated. PP00124 showed to be an efficient catalyst for the degradation of 4-hydroxyestradiol (4-OHE2), a carcinogenic hydroxylated derivate of E2. 4-OHE2 complete cleavage was obtained using PP00124 both in soluble form and in whole recombinant E. coli cells. LC–MS/MS analyses confirmed the generation of a semialdehyde product, through A-ring meta cleavage. To the best of our knowledge, PP00124 is the first characterized enzyme able to directly degrade 4-OHE2 via meta cleavage. Moreover, the complete 4-OHE2 biodegradation using recombinant whole cells highlighted advantages for bioremediation purposes.
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- 2023
- Full Text
- View/download PDF
6. Putative native South Amerindian origin of head lice clade F: evidence from head lice nits infesting human shrunken heads
- Author
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Amanzougaghene, Nadia, Charlier, Philippe, Fenollar, Florence, Raoult, Didier, and Mediannikov, Oleg
- Published
- 2022
- Full Text
- View/download PDF
7. Author Correction: HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
- Author
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Kolesnikova, Olga, Zachayus, Amélie, Pichard, Simon, Osz, Judit, Rochel, Natacha, Rossolillo, Paola, Kolb-Cheynel, Isabelle, Troffer-Charlier, Nathalie, Compe, Emmanuel, Bensaude, Olivier, Berger, Imre, and Poterszman, Arnaud
- Published
- 2022
- Full Text
- View/download PDF
8. Putative native South Amerindian origin of head lice clade F: evidence from head lice nits infesting human shrunken heads
- Author
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Nadia Amanzougaghene, Philippe Charlier, Florence Fenollar, Didier Raoult, and Oleg Mediannikov
- Subjects
Medicine ,Science - Abstract
Abstract The head louse, Pediculus humanus capitis, is a strictly obligate human ectoparasite with a long history of association with humans. Here, 17 ancient head lice nits were recovered from six shrunken human heads (known as tsantsas) of individuals from the Shuar/Jivaro tribe, a native Amazonian population from Ecuador, South America. Cytochrome b DNA analysis revealed the presence of three known mitochondrial clades. Clade A was the most frequent (52.94%), followed by F (35.29%), and B (11.76%). Eleven haplotypes were found in 17 samples, and nine of the haplotypes were novel, indicating an unusually high genetic diversity. In conclusion, we confirmed the presence of clades A, B and F in South Amerindian population. Moreover, the description of clade F, together with its previous reports in another Amerindian population from French Guiana, strongly support the hypothesis of a native South American origin for this clade, and probably derived from clade B which was carried to America by an ancestral Eurasian Beringian population. Further support to our conclusion and new insights might come from the analysis of a larger collection of modern and ancient native American lice.
- Published
- 2022
- Full Text
- View/download PDF
9. AD Course Map charts Alzheimer’s disease progression
- Author
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Igor Koval, Alexandre Bône, Maxime Louis, Thomas Lartigue, Simona Bottani, Arnaud Marcoux, Jorge Samper-González, Ninon Burgos, Benjamin Charlier, Anne Bertrand, Stéphane Epelbaum, Olivier Colliot, Stéphanie Allassonnière, and Stanley Durrleman
- Subjects
Medicine ,Science - Abstract
Abstract Alzheimer’s disease (AD) is characterized by the progressive alterations seen in brain images which give rise to the onset of various sets of symptoms. The variability in the dynamics of changes in both brain images and cognitive impairments remains poorly understood. This paper introduces AD Course Map a spatiotemporal atlas of Alzheimer’s disease progression. It summarizes the variability in the progression of a series of neuropsychological assessments, the propagation of hypometabolism and cortical thinning across brain regions and the deformation of the shape of the hippocampus. The analysis of these variations highlights strong genetic determinants for the progression, like possible compensatory mechanisms at play during disease progression. AD Course Map also predicts the patient’s cognitive decline with a better accuracy than the 56 methods benchmarked in the open challenge TADPOLE. Finally, AD Course Map is used to simulate cohorts of virtual patients developing Alzheimer’s disease. AD Course Map offers therefore new tools for exploring the progression of AD and personalizing patients care.
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- 2021
- Full Text
- View/download PDF
10. AD Course Map charts Alzheimer’s disease progression
- Author
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Koval, Igor, Bône, Alexandre, Louis, Maxime, Lartigue, Thomas, Bottani, Simona, Marcoux, Arnaud, Samper-González, Jorge, Burgos, Ninon, Charlier, Benjamin, Bertrand, Anne, Epelbaum, Stéphane, Colliot, Olivier, Allassonnière, Stéphanie, and Durrleman, Stanley
- Published
- 2021
- Full Text
- View/download PDF
11. An improved reversible watermarking scheme using embedding optimization and quaternion moments.
- Author
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Tahiri, Mohamed Amine, Karmouni, Hicham, Sayyouri, Mhamed, Qjidaa, Hassan, Ahmad, Musheer, Hammad, Mohamed, Pławiak, Paweł, Alfarraj, Osama, and El-Latif, Ahmed A. Abd
- Subjects
OPTIMIZATION algorithms ,DIGITAL watermarking ,SINGULAR value decomposition ,DISCRETE wavelet transforms ,COPYRIGHT ,DIGITAL image watermarking - Abstract
Digital watermarking of images is an essential method for copyright protection and image security. This paper presents an innovative, robust watermarking system for color images based on moment and wavelet transformations, algebraic decompositions, and chaotic systems. First, we extended classical Charlier moments to quaternary Charlier moments (QCM) using quaternion algebra. This approach eliminates the need to decompose color images before applying the discrete wavelet transform (DWT), reducing the computational load. Next, we decompose the resulting DWT matrix using QR and singular value decomposition (SVD). To enhance the system's security and robustness, we introduce a modified version of Henon's 2D chaotic map. Finally, we integrate the arithmetic optimization algorithm to ensure dynamic and adaptive watermark insertion. Our experimental results demonstrate that our approach outperforms current color image watermarking methods in security, storage capacity, and resistance to various attacks, while maintaining a high level of invisibility. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
12. Author Correction: HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
- Author
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Olga Kolesnikova, Amélie Zachayus, Simon Pichard, Judit Osz, Natacha Rochel, Paola Rossolillo, Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Emmanuel Compe, Olivier Bensaude, Imre Berger, and Arnaud Poterszman
- Subjects
Medicine ,Science - Published
- 2022
- Full Text
- View/download PDF
13. Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity
- Author
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Azarkan, Mohamed, Maquoi, Erik, Delbrassine, François, Herman, Raphael, M’Rabet, Nasiha, Calvo Esposito, Rafaèle, Charlier, Paulette, and Kerff, Frédéric
- Published
- 2020
- Full Text
- View/download PDF
14. Alkyladenine DNA glycosylase deficiency uncouples alkylation-induced strand break generation from PARP-1 activation and glycolysis inhibition
- Author
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Alhumaydhi, Fahad A., de O. Lopes, Debora, Bordin, Diana L., Aljohani, Abdullah S. M., Lloyd, Cameron B., McNicholas, Michael D., Milano, Larissa, Charlier, Clara F., Villela, Izabel, Henriques, João Antonio P., Plant, Kathryn E., Elliott, Ruan M., and Meira, Lisiane B.
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- 2020
- Full Text
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15. Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem
- Author
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Azarkan, Mohamed, Feller, Georges, Vandenameele, Julie, Herman, Raphaël, El Mahyaoui, Rachida, Sauvage, Eric, Vanden Broeck, Arnaud, Matagne, André, Charlier, Paulette, and Kerff, Frédéric
- Published
- 2018
- Full Text
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16. Assessment of DNA-PKcs kinase activity by quantum dot–based microarray
- Author
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Lafont, Florian, Ayadi, Nizar, Charlier, Cathy, Weigel, Pierre, Nabiev, Igor, Benhelli-Mokrani, Houda, and Fleury, Fabrice
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- 2018
- Full Text
- View/download PDF
17. HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
- Author
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Olga Kolesnikova, Amélie Zachayus, Simon Pichard, Judit Osz, Natacha Rochel, Paola Rossolillo, Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Emmanuel Compe, Olivier Bensaude, Imre Berger, Arnaud Poterszman, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), EMBL Heidelberg, Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Bristol [Bristol], Rochel, Natacha, Centre for Integrative Biology - CBI (Inserm U964 - CNRS UMR7104 - IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), European Molecular Biology Laboratory [Heidelberg] (EMBL), ANR-12-BSV8-0015,TFIIH-Complexes,Structure, fonction et dynamique du facteur de transcription TFIIH(2012), ANR-20-CE12-0017,TFIIH,Fonctionnement et dysfonctionnement du facteur de transcription/réparation de l'ADN TFIIH(2020), ANR-10-LABX-0030,INRT,Integrative Biology : Nuclear dynamics- Regenerative medicine - Translational medicine(2010), ANR-10-IDEX-0002,UNISTRA,Par-delà les frontières, l'Université de Strasbourg(2010), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), and European Project: 731005,H2020-INFRADEV-2016-1,INSTRUCT-ULTRA(2017)
- Subjects
Multidisciplinary ,Science ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Bristol BioDesign Institute ,[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Medicine ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology - Abstract
The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette into the baculoviral genome. This allows a rigorous characterization of recombinant bacmids but involves multiple steps, a limitation when many constructs are to be tested. For parallel expression screening and potential high throughput applications, we have established an open source multigene-expression toolbox exploiting homologous recombination, thus reducing the recombinant baculovirus generation to a single-step procedure and shortening the time from cloning to protein production to 2 weeks. The HR-bac toolbox is composed of a set of engineered bacmids expressing a fluorescent marker to monitor virus propagation and a library of transfer vectors. They contain single or dual expression cassettes bearing different affinity tags and their design facilitates the mix and match utilization of expression units from Multibac constructs. The overall cost of virus generation with HR-bac toolbox is relatively low as the preparation of linearized baculoviral DNA only requires standard reagents. Various multiprotein assemblies (nuclear hormone receptor heterodimers, the P-TEFb or the ternary CAK kinase complex associated with the XPD TFIIH subunit) are used as model systems to validate the toolbox presented.
- Published
- 2021
18. HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system.
- Author
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Olga, Kolesnikova, Amélie, Zachayus, Simon, Pichard, Judit, Osz, Natacha, Rochel, Paola, Rossolillo, Isabelle, Kolb-Cheynel, Nathalie, Troffer-Charlier, Emmanuel, Compe, Olivier, Bensaude, Imre, Berger, and Arnaud, Poterszman
- Subjects
HIGH throughput screening (Drug development) ,NUCLEAR receptors (Biochemistry) - Abstract
The Baculovirus/insect cell expression system is a powerful technology for reconstitution of eukaryotic macromolecular assemblies. Most multigene expression platforms rely on Tn7-mediated transposition for transferring the expression cassette into the baculoviral genome. This allows a rigorous characterization of recombinant bacmids but involves multiple steps, a limitation when many constructs are to be tested. For parallel expression screening and potential high throughput applications, we have established an open source multigene-expression toolbox exploiting homologous recombination, thus reducing the recombinant baculovirus generation to a single-step procedure and shortening the time from cloning to protein production to 2 weeks. The HR-bac toolbox is composed of a set of engineered bacmids expressing a fluorescent marker to monitor virus propagation and a library of transfer vectors. They contain single or dual expression cassettes bearing different affinity tags and their design facilitates the mix and match utilization of expression units from Multibac constructs. The overall cost of virus generation with HR-bac toolbox is relatively low as the preparation of linearized baculoviral DNA only requires standard reagents. Various multiprotein assemblies (nuclear hormone receptor heterodimers, the P-TEFb or the ternary CAK kinase complex associated with the XPD TFIIH subunit) are used as model systems to validate the toolbox presented. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
19. Putative native South Amerindian origin of head lice clade F: evidence from head lice nits infesting human shrunken heads
- Author
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Nadia, Amanzougaghene, Philippe, Charlier, Florence, Fenollar, Didier, Raoult, and Oleg, Mediannikov
- Subjects
Pediculus ,Animals ,Genetic Variation ,Humans ,Cytochromes b ,Lice Infestations ,Phylogeny - Abstract
The head louse, Pediculus humanus capitis, is a strictly obligate human ectoparasite with a long history of association with humans. Here, 17 ancient head lice nits were recovered from six shrunken human heads (known as tsantsas) of individuals from the Shuar/Jivaro tribe, a native Amazonian population from Ecuador, South America. Cytochrome b DNA analysis revealed the presence of three known mitochondrial clades. Clade A was the most frequent (52.94%), followed by F (35.29%), and B (11.76%). Eleven haplotypes were found in 17 samples, and nine of the haplotypes were novel, indicating an unusually high genetic diversity. In conclusion, we confirmed the presence of clades A, B and F in South Amerindian population. Moreover, the description of clade F, together with its previous reports in another Amerindian population from French Guiana, strongly support the hypothesis of a native South American origin for this clade, and probably derived from clade B which was carried to America by an ancestral Eurasian Beringian population. Further support to our conclusion and new insights might come from the analysis of a larger collection of modern and ancient native American lice.
- Published
- 2021
20. TCTP contains a BH3-like domain, which instead of inhibiting, activates Bcl-xL
- Author
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Thébault, Stéphanie, Agez, Morgane, Chi, Xiaoke, Stojko, Johann, Cura, Vincent, Telerman, Stéphanie B., Maillet, Laurent, Gautier, Fabien, Billas-Massobrio, Isabelle, Birck, Catherine, Troffer-Charlier, Nathalie, Karafin, Teele, Honoré, Joane, Senff-Ribeiro, Andrea, Montessuit, Sylvie, Johnson, Christopher M., Juin, Philippe, Cianférani, Sarah, Martinou, Jean-Claude, Andrews, David W., Amson, Robert, Telerman, Adam, and Cavarelli, Jean
- Published
- 2016
- Full Text
- View/download PDF
21. Structures of the free and inhibitors-bound forms of bromelain and ananain from Ananas comosus stem and in vitro study of their cytotoxicity
- Author
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Mohamed Azarkan, Erik Maquoi, François Delbrassine, Raphael Herman, Nasiha M’Rabet, Rafaèle Calvo Esposito, Paulette Charlier, and Frédéric Kerff
- Subjects
Models, Molecular ,Spectrometry, Mass, Electrospray Ionization ,Plant Stems ,Protein Conformation ,lcsh:R ,lcsh:Medicine ,Addiction ,Ananas ,Cysteine Proteinase Inhibitors ,Tosyllysine Chloromethyl Ketone ,Antineoplastic Agents, Phytogenic ,Bromelains ,Article ,Substrate Specificity ,Cysteine Endopeptidases ,Leucine ,Catalytic Domain ,Cell Line, Tumor ,Humans ,Diseases of the nervous system ,lcsh:Q ,Cysteine ,Disulfides ,lcsh:Science ,Neuroscience - Abstract
The Ananas comosus stem extract is a complex mixture containing various cysteine proteases of the C1A subfamily, such as bromelain and ananain. This mixture used for centuries in Chinese medicine, has several potential therapeutic applications as anti-cancer, anti-inflammatory and ecchymosis degradation agent. In the present work we determined the structures of bromelain and ananain, both in their free forms and in complex with the inhibitors E64 and TLCK. These structures combined with protease-substrate complexes modeling clearly identified the Glu68 as responsible for the high discrimination of bromelain in favor of substrates with positively charged residues at P2, and unveil the reasons for its weak inhibition by cystatins and E64. Our results with purified and fully active bromelain, ananain and papain show a strong reduction of cell proliferation with MDA-MB231 and A2058 cancer cell lines at a concentration of about 1 μM, control experiments clearly emphasizing the need for proteolytic activity. In contrast, while bromelain and ananain had a strong effect on the proliferation of the OCI-LY19 and HL-60 non-adherent cell lines, papain, the archetypal member of the C1A subfamily, had none. This indicates that, in this case, sequence/structure identity beyond the active site of bromelain and ananain is more important than substrate specificity.
- Published
- 2020
22. Alkyladenine DNA glycosylase deficiency uncouples alkylation-induced strand break generation from PARP-1 activation and glycolysis inhibition
- Author
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Larissa Milano, Ruan M. Elliott, Izabel Vianna Villela, Kathryn E. Plant, Michael D. McNicholas, Clara F. Charlier, Fahad A. Alhumaydhi, Lisiane B. Meira, Diana L. Bordin, João Antonio Pêgas Henriques, Abdullah S. M. Aljohani, Cameron B. Lloyd, and Débora de Oliveira Lopes
- Subjects
Alkylation ,DNA Repair ,DNA repair ,Poly ADP ribose polymerase ,Primary Cell Culture ,Nicotinamide phosphoribosyltransferase ,Poly (ADP-Ribose) Polymerase-1 ,lcsh:Medicine ,Biochemistry ,Article ,DNA Glycosylases ,chemistry.chemical_compound ,Glycolysis Inhibition ,Mice ,Piperidines ,polycyclic compounds ,Animals ,lcsh:Science ,Nicotinamide Phosphoribosyltransferase ,Cells, Cultured ,Mice, Knockout ,Acrylamides ,Multidisciplinary ,lcsh:R ,DNA Breaks ,Base excision repair ,DNA ,Fibroblasts ,Methyl Methanesulfonate ,NAD ,female genital diseases and pregnancy complications ,Methyl methanesulfonate ,Cell biology ,DNA Alkylation ,chemistry ,Cytokines ,lcsh:Q ,NAD+ kinase ,Glycolysis - Abstract
DNA alkylation damage is repaired by base excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG). Despite its role in DNA repair, AAG-initiated BER promotes cytotoxicity in a process dependent on poly (ADP-ribose) polymerase-1 (PARP-1); a NAD+-consuming enzyme activated by strand break intermediates of the AAG-initiated repair process. Importantly, PARP-1 activation has been previously linked to impaired glycolysis and mitochondrial dysfunction. However, whether alkylation affects cellular metabolism in the absence of AAG-mediated BER initiation is unclear. To address this question, we temporally profiled repair and metabolism in wild-type and Aag−/− cells treated with the alkylating agent methyl methanesulfonate (MMS). We show that, although Aag−/− cells display similar levels of alkylation-induced DNA breaks as wild type, PARP-1 activation is undetectable in AAG-deficient cells. Accordingly, Aag−/− cells are protected from MMS-induced NAD+ depletion and glycolysis inhibition. MMS-induced mitochondrial dysfunction, however, is AAG-independent. Furthermore, treatment with FK866, a selective inhibitor of the NAD+ salvage pathway enzyme nicotinamide phosphoribosyltransferase (NAMPT), synergizes with MMS to induce cytotoxicity and Aag−/− cells are resistant to this combination FK866 and MMS treatment. Thus, AAG plays an important role in the metabolic response to alkylation that could be exploited in the treatment of conditions associated with NAD+ dysregulation.
- Published
- 2020
23. An improved reversible watermarking scheme using embedding optimization and quaternion moments
- Author
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Mohamed Amine Tahiri, Hicham Karmouni, Mhamed Sayyouri, Hassan Qjidaa, Musheer Ahmad, Mohamed Hammad, Paweł Pławiak, Osama Alfarraj, and Ahmed A. Abd El-Latif
- Subjects
Henon chaotic map ,Quaternion moments ,Digital image watermarking ,Optimization algorithm ,Medicine ,Science - Abstract
Abstract Digital watermarking of images is an essential method for copyright protection and image security. This paper presents an innovative, robust watermarking system for color images based on moment and wavelet transformations, algebraic decompositions, and chaotic systems. First, we extended classical Charlier moments to quaternary Charlier moments (QCM) using quaternion algebra. This approach eliminates the need to decompose color images before applying the discrete wavelet transform (DWT), reducing the computational load. Next, we decompose the resulting DWT matrix using QR and singular value decomposition (SVD). To enhance the system's security and robustness, we introduce a modified version of Henon's 2D chaotic map. Finally, we integrate the arithmetic optimization algorithm to ensure dynamic and adaptive watermark insertion. Our experimental results demonstrate that our approach outperforms current color image watermarking methods in security, storage capacity, and resistance to various attacks, while maintaining a high level of invisibility.
- Published
- 2024
- Full Text
- View/download PDF
24. Assessment of DNA-PKcs kinase activity by quantum dot-based microarray
- Author
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Florian, Lafont, Nizar, Ayadi, Cathy, Charlier, Pierre, Weigel, Igor, Nabiev, Houda, Benhelli-Mokrani, and Fabrice, Fleury
- Subjects
enzymes and coenzymes (carbohydrates) ,DNA End-Joining Repair ,DNA Repair ,Neoplasms ,Quantum Dots ,Nuclear Proteins ,Antineoplastic Agents ,DNA Breaks, Double-Stranded ,DNA-Activated Protein Kinase ,Microarray Analysis ,Protein Kinase Inhibitors ,Antibodies ,Article - Abstract
Therapeutic efficacy against cancer is often based on a variety of DNA lesions, including DNA double-strand breaks (DSBs) which are repaired by homologous recombination and non-homologous end joining (NHEJ) pathways. In the past decade, the functions of the DNA repair proteins have been described as a potential mechanism of resistance in tumor cells. Therefore, the DNA repair proteins have become targets to improve the efficacy of anticancer therapy. Given the central role of DNA-PKcs in NHEJ, the therapeutic efficacy of targeting DNA-PKcs is frequently described as a strategy to prevent repair of treatment-induced DNA damage in cancer cells. The screening of a new inhibitor acting as a sensitizer requires the development of a high-throughput tool in order to identify and assess the most effective molecule. Here, we describe the elaboration of an antibody microarray dedicated to the NHEJ pathway that we used to evaluate the DNA-PKcs kinase activity in response to DNA damage. By combining a protein microarray with Quantum-Dot detection, we show that it is possible to follow the modification of phosphoproteomic cellular profiles induced by inhibitors during the response to DNA damage. Finally, we discuss the promising tool for screening kinase inhibitors and targeting DSB repair to improve cancer treatment.
- Published
- 2018
25. An efficient watermarking algorithm for digital audio data in security applications.
- Author
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Yamni, Mohamed, Daoui, Achraf, Karmouni, Hicham, Sayyouri, Mhamed, Qjidaa, Hassan, motahhir, Saad, Jamil, Ouazzani, El-Shafai, Walid, Algarni, Abeer D., Soliman, Naglaa F., and Aly, Moustafa H.
- Subjects
DIGITAL watermarking ,DIGITAL audio ,DATA security ,RASPBERRY Pi ,SOUND systems ,COMPUTER workstation clusters - Abstract
Transform-domain audio watermarking systems are more robust than time-domain systems. However, the main weakness of these systems is their high computational cost, especially for long-duration audio signals. Therefore, they are not desirable for real-time security applications where speed is a critical factor. In this paper, we propose a fast watermarking system for audio signals operating in the hybrid transform domain formed by the fractional Charlier transform (FrCT) and the dual-tree complex wavelet transform (DTCWT). The central idea of the proposed algorithm is to parallelize the intensive and repetitive steps in the audio watermarking system and then implement them simultaneously on the available physical cores on an embedded systems cluster. In order to have a low power consumption and a low-cost cluster with a large number of physical cores, four Raspberry Pis 4B are used where the communication between them is ensured using the Message Passing Interface (MPI). The adopted Raspberry Pi cluster is also characterized by its portability and mobility, which are required in watermarking-based smart city applications. In addition to its resistance to any possible manipulation (intentional or unintentional), high payload capacity, and high imperceptibility, the proposed parallel system presents a temporal improvement of about 70%, 80%, and 90% using 4, 8, and 16 physical cores of the adopted cluster, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
26. Charge transport through one-dimensional Moiré crystals
- Author
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Jean-Christophe Charlier, Maria Luisa Della Rocca, P. Lafarge, Aurélien Lherbier, Clément Barraud, Roméo Bonnet, and UCL - SST/IMCN/NAPS - Nanoscopic Physics
- Subjects
Multidisciplinary ,Valence (chemistry) ,Materials science ,Condensed matter physics ,Graphene ,Superlattice ,02 engineering and technology ,Moiré pattern ,Carbon nanotube ,Electronic structure ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,021001 nanoscience & nanotechnology ,Bioinformatics ,01 natural sciences ,Article ,law.invention ,Crystal ,Condensed Matter::Materials Science ,law ,0103 physical sciences ,010306 general physics ,0210 nano-technology ,Quantum - Abstract
Moiré superlattices were generated in two-dimensional (2D) van der Waals heterostructures and have revealed intriguing electronic structures. The appearance of mini-Dirac cones within the conduction and valence bands of graphene is one of the most striking among the new quantum features. A Moiré superstructure emerges when at least two periodic sub-structures superimpose. 2D Moiré patterns have been particularly investigated in stacked hexagonal 2D atomic lattices like twisted graphene layers and graphene deposited on hexagonal boron-nitride. In this letter, we report both experimentally and theoretically evidence of superlattices physics in transport properties of one-dimensional (1D) Moiré crystals. Rolling-up few layers of graphene to form a multiwall carbon nanotube adds boundaries conditions that can be translated into interference fringes-like Moiré patterns along the circumference of the cylinder. Such a 1D Moiré crystal exhibits a complex 1D multiple bands structure with clear and robust interband quantum transitions due to the presence of mini-Dirac points and pseudo-gaps. Our devices consist in a very large diameter (>80 nm) multiwall carbon nanotubes of high quality, electrically connected by metallic electrodes acting as charge reservoirs. Conductance measurements reveal the presence of van Hove singularities assigned to 1D Moiré superlattice effect and illustrated by electronic structure calculations.
- Published
- 2016
27. The embalmed heart of Richard the Lionheart (1199 A.D.): a biological and anthropological analysis
- Author
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Geoffroy Lorin de la Grandmaison, Dominique Favier, Caroline Dorion-Peyronnet, Christophe Moulherat, Philippe Charlier, Raphaël Weil, Isabelle Huynh-Charlier, Ana Maria Lazar, Joël Poupon, Christian Hervé, Gaël François Jeannel, and Speranta Maria Popescu
- Subjects
Multidisciplinary ,Embalming ,business.industry ,Myocardium ,Preservation, Biological ,Heart ,Mummies ,History, Medieval ,Mass Spectrometry ,Article ,Current practice ,Anthropology ,Medicine ,Humans ,Middle Ages ,Oleanolic Acid ,business ,Classics - Abstract
During the Middle Ages, the partition of the cadaver of the elite members was a current practice, with highly technical treatment given to symbolic organs such as the heart. Considered mostly from a theoretical point of view, this notion of dilaceratio corporis has never been biologically explored. To assess the exact kind of embalming reserved to the heart, we performed a full biomedical analysis of the mummified heart of the English King Richard I (1199 A.D.). Here we show among other aspects, that the organ has been embalmed using substances inspired by Biblical texts and practical necessities of desiccation. We found that the heart was deposed in linen, associated with myrtle, daisy, mint, frankincense, creosote, mercury and, possibly, lime. Furthermore, the goal of using such preservation materials was to allow long-term conservation of the tissues, and good-smelling similar to the one of the Christ (comparable to the odor of sanctity).
- Published
- 2012
28. Molecular nano-I-beam class of materials: options based on configuration, first principles-based optimization and properties.
- Author
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Elmoselhy SAM
- Abstract
Nanotubes showed merits including high structural strength-to-weight ratio. However, tubes are less favored regarding stiffness and strength. Nano-I-beams are proposed for improved nano-mechanics. Computationally, the study proposes novel molecular designs of I-beam-like shaped structures. A conformation analysis, molecular dynamics and first principles-based optimization are presented. The study proposes options based on the configuration of the molecular nano-I-beam structure providing less number of planes of symmetry and hence more stability than nanotube-like structures. These designs feature a unique geometrical differentiator of having the walls of the out-of-plane hexagonal motif-based molecular nano-I-beam (C
60 H46 ) inclined with different inclination angles enabling promising properties. The stability of the proposed nano-I-beam is proved on par with the corresponding nanotube-like structure. First principles-based evidence is provided on the comparable polarizability and the comparable ability to store energy of the supercell of the crystalline slab nano-I-beam in comparison with the corresponding nanotube. A proposed hybrid octa-hexagonal-cubic molecular nano-I-beam (C24 H12 ) remedies the nano-buckling observed in the alike square-octagonal nanostructure. The molecular nano-I-beam exhibits intrinsic switchability that enables the nano-I-beam to be a topological semiconductor/insulator. The results show promising electronic and elastic properties of the proposed nano-I-beams that suit several applications such as their use in capacitors, transistors, insulators, batteries, quantization-based nano-devices, solid lubricant additive to grease, toughening fibers of nanocomposites, hydrophobic films, emissions adsorbents, catalytic sensors, PAH materials for space, and sustainable energy. The molecular nano-I-beam provides the base of the corresponding 2-D crystalline slab nano-I-beams., (© 2024. The Author(s).)- Published
- 2024
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29. Charge transport through one-dimensional Moiré crystals
- Author
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Bonnet, Roméo, primary, Lherbier, Aurélien, additional, Barraud, Clément, additional, Rocca, Maria Luisa Della, additional, Lafarge, Philippe, additional, and Charlier, Jean-Christophe, additional
- Published
- 2016
- Full Text
- View/download PDF
30. The embalmed heart of Richard the Lionheart (1199 A.D.): a biological and anthropological analysis
- Author
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Charlier, Philippe, primary, Poupon, Joël, additional, Jeannel, Gaël-François, additional, Favier, Dominique, additional, Popescu, Speranta-Maria, additional, Weil, Raphaël, additional, Moulherat, Christophe, additional, Huynh-Charlier, Isabelle, additional, Dorion-Peyronnet, Caroline, additional, Lazar, Ana-Maria, additional, Hervé, Christian, additional, and de la Grandmaison, Geoffroy Lorin, additional
- Published
- 2013
- Full Text
- View/download PDF
31. Genomic analysis of the blood attributed to Louis XVI (1754-1793), king of France.
- Author
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Olalde, Iñigo, SĊnchez-Quinto, Federico, Datta, Debayan, Marigorta, Urko M., Chiang, Charleston W. K., Rodrǰguez, Juan Antonio, Fernández-Callejo, Marcos, González, Irene, Montfort, Magda, Matas-Lalueza, Laura, Civit, Sergi, Luiselli, Donata, Charlier, Philippe, Pettener, Davide, RamĊrez, Oscar, Navarro, Arcadi, Himmelbauer, Heinz, Marqués-Bonet, Tomÿs, and Lalueza-Fox, Carles
- Subjects
GENOMES ,GENETIC genealogy ,PYROGRAPHY ,BEHEADING - Abstract
A pyrographically decorated gourd, dated to the French Revolution period, has been alleged to contain a handkerchief dipped into the blood of the French king Louis XVI (1754-1793) after his beheading but recent analyses of living males from two Bourbon branches cast doubts on its authenticity. We sequenced the complete genome of the DNA contained in the gourd at low coverage (,2.53) with coding sequences enriched at a higher ,7.33 coverage. We found that the ancestry of the gourd's genome does not seem compatible with Louis XVI's known ancestry. From a functional perspective, we did not find an excess of alleles contributing to height despite being described as the tallest person in Court. In addition, the eye colour prediction supported brown eyes, while Louis XVI had blue eyes. This is the first draft genome generated from a person who lived in a recent historical period; however, our results suggest that this sample may not correspond to the alleged king. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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32. Multimode Friedel oscillations in monolayer and bilayer graphene.
- Author
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Lin CY and Chiu CW
- Abstract
This study systematically explores the influence of charged impurities on static screening in monolayer graphene and extends the investigation to AA-stacked and AB-stacked bilayer graphene (BLG). Applying the random phase approximation (RPA), monolayer graphene displays unique beating Friedel oscillations (FOs) in inter-valley and intra-valley channels. Shifting to BLG, the study emphasizes layer-specific responses on each layer by considering self-consistent field interactions between layers. It also explores the derived multimode FOs, elucidating distinctions from monolayer behavior. In AA-stacked BLG, distinct metallic screening behaviors are revealed, uncovering unique oscillatory patterns in induced charge density, providing insights into static Coulomb scattering effects between two Dirac cones. The exploration extends to AB-stacked BLG, unveiling layer-specific responses of parabolic bands in multimode FOs with increasing Fermi energy. This comprehensive investigation, integrating RPA considerations, significantly advances our understanding of layer-dependent static screening in the broader context of FOs in graphene, providing valuable contributions to the field of condensed matter physics., (© 2024. The Author(s).)
- Published
- 2024
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33. Identification of individual and few layers of WS2 using Raman Spectroscopy
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Berkdemir, Ayse, primary, Gutiérrez, Humberto R., additional, Botello-Méndez, Andrés R., additional, Perea-López, Néstor, additional, Elías, Ana Laura, additional, Chia, Chen-Ing, additional, Wang, Bei, additional, Crespi, Vincent H., additional, López-Urías, Florentino, additional, Charlier, Jean-Christophe, additional, Terrones, Humberto, additional, and Terrones, Mauricio, additional
- Published
- 2013
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- View/download PDF
34. Nitrogen-doped graphene: beyond single substitution and enhanced molecular sensing
- Author
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Lv, Ruitao, primary, Li, Qing, additional, Botello-Méndez, Andrés R., additional, Hayashi, Takuya, additional, Wang, Bei, additional, Berkdemir, Ayse, additional, Hao, Qingzhen, additional, Elías, Ana Laura, additional, Cruz-Silva, Rodolfo, additional, Gutiérrez, Humberto R., additional, Kim, Yoong Ahm, additional, Muramatsu, Hiroyuki, additional, Zhu, Jun, additional, Endo, Morinobu, additional, Terrones, Humberto, additional, Charlier, Jean-Christophe, additional, Pan, Minghu, additional, and Terrones, Mauricio, additional
- Published
- 2012
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- View/download PDF
35. Experimental analysis of rotating bridge structural responses to existing railway train loads via time-frequency and Hilbert-Huang transform energy spectral analysis.
- Author
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Liu X, Wu H, Zhao S, and Yang X
- Abstract
With the rapid development of national infrastructure projects, there has been a significant increase in intersecting lines in transportation construction. As a result, rotating bridges are increasingly used in engineering projects that span existing railway lines. In order to study the spatial response characteristics and vibration wave transmission mechanisms of the rotating bridge structure under the loading of existing railway trains, field experiments and numerical analyses were conducted. The response characteristics of these bridges were investigated under different types and speeds of adjacent existing lines. A comprehensive methodology has been proposed, integrating the time domain spectrum and the Hilbert-Huang Transform (HHT) energy spectrum for signal processing and vibration analysis. The analysis was carried out using MATLAB 2018a software. This methodology was applied to analyze the test data. The results show that significant resonance phenomenon occurs in the girders of the rotating bridge under the loading of trains on the existing line. The low-frequency component f
1 (2-5 Hz) is the primary factor contributing to the amplification of the acceleration response in the rotating bridge, while f3 (10-13 Hz) plays a secondary role. The frequency distribution characteristics of vibration waves caused by train loads on the existing line have a significant influence on the acceleration response of the rotating bridge's girders. The predominant frequency of vibration waves at each measuring point along the transmission path shows a trend of decreasing → increasing → decreasing. The impact on the rotating bridge structure of vibration waves generated by low-speed freight trains on existing railways is greater. The research findings are of great importance for studying the dynamic response of rotating bridges adjacent to existing railway lines., (© 2024. The Author(s).)- Published
- 2024
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- View/download PDF
36. Rational design and synthesis of new pyrrolone candidates as prospective insecticidal agents against Culex pipiens L. Larvae.
- Author
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Hekal, Mohamed H., Hashem, Ahmed I., El-Azm, Fatma S.M. Abu, Abdel-Haleem, Doaa R., Rafat, El-Hady, and Ali, Yasmeen M.
- Abstract
As a result of its high reactivity, furan-2(3H)-one derivative 2 can be selected as a versatile and suitable candidate for building of novel nitrogen heterocyclic compounds. Consequently, furan-2(3H)-one derivative 2 and some nitrogen nucleophiles were utilized as starting materials for the formation of new pyridazinone and pyrrolone derivatives bearing naphthalene moiety. The continuous buildup of insecticide resistance is the main obstacle facing pest control measures. Pyrrole-based insecticides are a favourable choice due to their unique mode of action and no cross-resistance with traditional neurotoxic insecticides. The larvicidal activities of pyrrolone derivatives were assessed against field and laboratory strains of Culex pipiens larvae in comparison with chlorfenapyr (pyrrole insecticide). Compounds 17 (21.05 µg/mL) > 9 (22.81 µg/mL) > 15 (24.39 µg/mL) > 10 (26.76 µg/mL) > 16 (32.09 µg/mL) were most effective against lab strain of C. pipiens larvae relative to chlorfenapyr (25.43 µg/mL). While in field strain, 17 and 15 were the most toxic compounds followed by 9 > 10 > 16 > 2 with LC
50 of 9.87, 10.76, 11.52, 12.68, 15.32 and 18.37 µg/mL, respectively, compared with chlorfenapyr with 14.03 µg/mL. The cytochrome P-450 monooxygenase activities were significantly increased in treated larvae of lab and field strains relative to untreated. The great variations in toxicity of the synthesized compounds were interpreted by structure-activity relationship study. The pyrrolone derivatives are effective against field and insecticide-resistant strains. Therefore, they are considered promising compounds to be integrated into pest management programs. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
37. Distance based topological characterization, graph energy prediction, and NMR patterns of benzene ring embedded in P-type surface in 2D network.
- Author
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Zhang, Xiujun, Prabhu, S., Arulperumjothi, M., Prabhu, S. Manikanda, Arockiaraj, Micheal, and Manimozhi, V.
- Subjects
MOLECULAR connectivity index ,MINIATURE objects ,MOLECULAR graphs ,CARBON nanotubes ,QSAR models - Abstract
Nanostructures are tiny objects at the molecular and microscopic scale, with carbon nanotubes being the most notable among them. The elements possess exceptional microelectronic properties and other unique characteristics. Researchers have recently focused on the mathematical features of these materials. Molecular descriptors are crucial in mathematical chemistry, particularly in QSAR and QSPR modeling. Topological indices hold a significant position among them. This study presents the precise formulation of the ten most crucial topological indices for a benzene ring positioned on a P-type surface within the highly symmetric 2D lattice BCZ 48 . We have incorporated the computed indices to develop a predictive model for the graph energy of the 2D lattice and, in addition, provided the NMR patterns and the HOMO-LUMO gap. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
38. RF NEMS switches based on graphene for low pull-in voltage and excellent RF performance.
- Author
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Zheng, Laifang, Zhang, Junsheng, Wang, Huajie, and Liu, Jijun
- Subjects
NANOELECTROMECHANICAL systems ,FINITE element method ,IMPEDANCE matching ,INSERTION loss (Telecommunication) ,DATA warehousing - Abstract
This study introduces a novel graphene RF NEMS capacitive switch and conducts an extensive analysis of its RF performance using CST and COMSOL Multiphysics software. The switch's characteristic impedance matching is accomplished through a coplanar waveguide (CPW) structure, incorporating a dielectric layer topped with conductive graphene beam electrodes. Simulation results reveal that the monolayer graphene RF NEMS switch maintains an insertion loss of 0.003 ~ 0.015 dB and an isolation greater than 40 dB across an ultra-wideband (UWB) frequency range of DC ~ 140 GHz. Moreover, the switch demonstrates a switching time of 4.03 ps at a pull-in voltage of 1 V. The performance of multilayer graphene RF NEMS switches was also evaluated. The study further investigates the operational mechanism of the graphene RF NEMS switch and performs finite element analysis on the proposed design. The graphene RF NEMS switch discussed herein offers significant advantages, including minimal size, lightweight, cost-effectiveness, low pull-in voltage, rapid response time, excellent RF performance, and reduced space consumption in circuitry. Its potential applications span a range from L to F bands, encompassing data storage, communication systems, sensors, remote sensing and military uses. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
39. Comparative LC–MS-based metabolite profiling, antioxidant, and antibacterial properties of Bunium bulbocastanum tubers from two regions in Algeria.
- Author
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Bouhalla, Asma-Warda, Benabdelmoumene, Djilali, Dahmouni, Said, Bengharbi, Zineb, Hellal, Khaoula, Qadi, Wasim S. M., Al-Olayan, Ebtesam, Moreno, Andres, Bekada, Ahmed, Buzgaia, Nawal, Aziz, Habsah, and Mediani, Ahmed
- Abstract
Traditional herbalists have been relied on for many years by Algerians to cure a wide range of diseases. Regardless of their nutritional values, mushrooms have chemical properties that make them attractive, beneficial, and more likely to be studied by researchers, according to ethnobotanical literature on traditional phytotherapy. Among all the edible mushrooms, tubers are a type of fungus that are traditionally used in fine dining and have garnered attention recently because of their many therapeutic applications. This research delves into a meticulous analysis of bioactive constituents in Bunium bulbocastanum tubers, sourced from Mostaganem and Relizane regions, with a keen focus on polyphenols, flavonoids, and condensed tannins. The quantification of total phenolic content was executed through the Folin-Ciocalteu assay, while flavonoids were assessed via the aluminum chloride colorimetric method. In addition, condensed tannins were evaluated in this study. Antioxidant capacities were scrutinized employing the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Microbial inhibition studies were conducted against five benchmark bacterial strains, utilizing the agar disc diffusion technique. Furthermore, a comprehensive liquid chromatography–mass spectrometry (LC–MS) analysis was performed to identify and quantify bioactive compounds. The findings underscore that the Mostaganem extracts were particularly rich in polyphenols (11.65 mg GAE/g of extract) and tannins (1.30 mg CE/g of extract), while the Relizane extracts boasted significant flavonoid concentrations (9.421 mg QE/g of extract). Notably, 4-methylguaiacol (82.04 mg/L), caffeic acid dimethyl ether (27.76 mg/L), syringic acid (20.48 mg/L), and naringenin (16.05 mg/L) emerged as the predominant volatile compounds. Compositional investigation of the extracts by LC–MS confirmed the presence of various compounds that were linked to the bioactivities exhibited by B. bulbocastanum tubers. These findings demonstrate the effective antibacterial and antioxidant properties of B. bulbocastanum tubers, indicating their potential use in pharmaceutical and nutraceutical applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
40. Association between urinary phthalate metabolites and Anemia in US adults.
- Author
-
Ma, Huimiao, Deng, Wenqi, Liu, Junxia, and Ding, Xiaoqing
- Subjects
IRON in the body ,HEALTH & Nutrition Examination Survey ,IRON metabolism ,LOGISTIC regression analysis ,LONGITUDINAL method - Abstract
Initial research indicates a possible connection between exposure to phthalates and the development of anemia. To fill the gap in epidemiological data, our study utilized data from across the United States, representative on a national scale, to evaluate the association between the concentration of phthalate metabolites in urine and both anemia and iron levels. We gathered data on 11,406 individuals from the National Health and Nutrition Examination Survey (NHANES) database, spanning 2003–2018. We conducted logistic and linear regression analyses, adjusted for potential confounding factors, to evaluate the correlations between different phthalate metabolites and anemia, as well as serum iron levels, including gender-stratified analysis. Most urinary phthalate metabolites were positively correlated with an increased risk of anemia, and the majority were negatively correlated with serum iron levels. The study revealed that for every unit increase in ln-transformed metabolite concentrations, the odds ratios (ORs) for anemia increased to varying degrees, depending on the phthalate: Monobutyl phthalate (MBP) at 1.08 (95% CI 1.01–1.17, P = 0.0314), mono(3-carboxypropyl) phthalate (MCPP) at 1.17 (95% CI 1.10–1.24, P < 0.0001), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) at 1.08 (95% CI 1.02–1.15, P = 0.0153), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) at 1.14 (95% CI 1.07–1.21, P < 0.0001), mono(2-ethyl-5-carboxypentyl) phthalate (MECPP) at 1.11 (95% CI 1.03–1.18, P = 0.0030), monocarboxynonyl phthalate (MCNP) at 1.11 (95% CI 1.03–1.19, p = 0.0050), and monocarboxyoctyl phthalate (MCOP) at 1.13 (95% CI 1.07–1.19, P < 0.0001). Increased levels of MBP, MEHP, MBzP, MCPP, MEHHP, MEOHP, MIBP, MECPP, MCNP, and MCOP were linked with changes in serum iron levels, ranging from − 0.99 µg/dL (95% CI − 1.69 to − 0.29) to − 3.72 µg/dL (95% CI − 4.32 to − 3.11). Mixed-exposure analysis shows consistency with single-exposure model. Further mediation analysis showed that the association between single urinary phthalates and the risk of anemia was mediated by serum iron with a mediation ratio of 24.34–95.48% (P < 0.05). The presence of phthalate metabolites in urine shows a positive correlation with the prevalence of anemia, which was possibly and partly mediated by iron metabolism. Nonetheless, to confirm a definitive causal link and comprehend the underlying mechanisms of how phthalate exposure influences anemia, additional longitudinal and experimental research is required. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
41. The impact mechanism of telework on job performance: a cross-level moderation model of digital leadership.
- Author
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Liao, Meihui, Li, Shiyuan, and Liu, Hongda
- Subjects
JOB performance ,TELECOMMUTING ,LEADERSHIP ,JOB security ,FAMILY conflict ,EMBEDDING theorems ,MEDIATION (Statistics) - Abstract
Traditional enterprise management believes that telecommuting activities are out of the enterprise's control, which may reduce staff performance. We use the extension of job demand-resource theory and work embeddedness theory to develop and test the intermediary mechanism of embedded in and out of work in telework. Moreover, it judges the mediating effect of job embeddedness on telecommuting → job performance. With the help of family conflict theory, we have revealed the possible performance changes in telework and the impact of family on telework. We predict embedding outside of work may reduce job performance. However, this worry will not happen under the adjustment of digital leadership and job insecurity. We collected survey data from 36 enterprise teams and 328 members. We have confirmed that work performance will not be reduced by telecommuting. Digital leadership magnifies the embedding of telecommuting resources into employees' work to a certain extent and inhibits the embedding problem outside work caused by telecommuting requirements. The telecommuting requirement may become a positive factor for employees staying home and avoiding workplace conflicts. We confirmed the inhibitory effect of job embeddedness on turnover rate and expanded the antecedent model of job embeddedness theory. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
42. The complexometric behavior of selected aroyl-S,N-ketene acetals shows that they are more than AIEgens.
- Author
-
Biesen, Lukas and Müller, Thomas J. J.
- Subjects
ACETAL resins ,METAL detectors ,JOB analysis ,METAL ions ,PHENANTHROLINE - Abstract
Using the established synthetic methods, aroyl-S,N-ketene acetals and subsequent bi- and multichromophores can be readily synthesized. Aside from pronounced AIE (aggregation induced emission) properties, these selected examples possess distinct complexometric behavior for various metals purely based on the underlying structural motifs. This affects the fluorescence properties of the materials which can be readily exploited for metal ion detection and for the formation of different metal-aroyl-S,N-ketene acetal complexes that were confirmed by Job plot analysis. In particular, gold(I), iron(III), and ruthenium (III) ions reveal complexation enhanced or quenched emission. For most dyes, weakly coodinating complexes were observed, only in case of a phenanthroline aroyl-S,N-ketene acetal multichromophore, measurements indicate the formation of a strongly coordinating complex. For this multichromophore, the complexation results in a loss of fluorescence intensity whereas for dimethylamino-aroyl-S,N-ketene acetals and bipyridine bichromophores, the observed quantum yield is nearly tripled upon complexation. Even if no stable complexes are formed, changes in absorption and emission properties allow for a simple ion detection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. Multi-wall carbon Nanotube surface-based functional nanoparticles for stimuli-responsive dual pharmaceutical compound delivery.
- Author
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Nabitabar, Masoumeh, Shaterian, Maryam, Danafar, Hossein, and Enhessari, Morteza
- Abstract
Carbon nanotubes (CNTs) have the potential to serve as delivery systems for medicinal substances and gene treatments, particularly in cancer treatment. Co-delivery of curcumin (CUR) and Methotrexate (MTX) has shown promise in cancer treatment, as it uses fewer drugs and has fewer side effects. This study used MTX-conjugated albumin (BSA)-based nanoparticles (BSA-MTX) to enhance and assess the efficiency of CUR. In-vitro cytotoxicity tests, DLS, TEM, FTIR, UV/Vis, SEM, and DSC studies assessed the formulations' physical and chemical properties. The Proteinase K enzyme was used to severe amidic linkages between MTX and BSA. The findings demonstrated the efficacy of using ƒ-MWCNT-CUR-BSA-MTX as a vehicle for efficient co-delivery of CUR and MTX in cancer treatment. The MTT colorimetric method was used to evaluate the effect of chemical and medicinal compounds. Cell division was studied using the MTT method to investigate the effect of pure MWCNT, pure CUR, MTX-BSA, and ƒ-MWCNT-CUR-MTX-BSA. Studies on cell lines have shown that the combination of curcumin and MTX with CNT can increase and improve the effectiveness of both drugs against cancer. A combination of drugs curcumin and methotrexate simultaneously had a synergistic effect on MCF-7 cells, which indicated that these drugs could potentially be used as a strategy for both prevention and treatment of breast cancer. Also, ƒ-MWCNT-CUR-MTX-BSA was found to have a significant effect on cancer treatment with minimal toxicity compared to pure curcumin, pure MTX-BSA, MTX, and ƒ-MWCNT alone. Unique properties such as a high ratio of specific surface area to volume, high chemical stability, chemical adsorption ability, high capacity of drug and biomolecules of carbon nanotubes, as well as multiple drug loading at the same time The combination of ƒ-MWCNT-CUR-BSA MTX significantly impacts cancer therapy), are desirable as an alternative option for targeted drug delivery and high therapeutic efficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
44. Occupational exposure, carcinogenic and non-carcinogenic risk assessment of formaldehyde in the pathology labs of hospitals in Iran.
- Author
-
Foroughi, Parvin, Golbabaei, Farideh, Sadeghi-Yarandi, Mohsen, Yaseri, Mehdi, Fooladi, Mahta, and Kalantary, Saba
- Subjects
OCCUPATIONAL exposure ,FORMALDEHYDE ,RISK assessment ,PATHOLOGY ,DISEASE risk factors ,HOSPITALS ,HOSPITAL laboratories ,PATHOLOGICAL laboratories - Abstract
Formaldehyde, a known carcinogenic compound, is commonly used in various medical settings. The objective of this study was to assess the carcinogenic and non-carcinogenic risks associated with occupational exposure to formaldehyde. This study was conducted in the pathology labs of four hospitals in Tehran. Cancer and non-cancer risks were evaluated using the quantitative risk assessment method proposed by the United States environmental protection agency (USEPA), along with its provided database known as the integrated risk information system (IRIS). Respiratory symptoms were assessed using the American thoracic society (ATS) questionnaire. The results indicated that 91.23% of exposure levels in occupational groups exceed the NIOSH standard of 0.016 ppm. Regarding carcinogenic risk, 41.03% of all the studied subjects were in the definite carcinogenic risk range (LCR > 10
−4 ), 23.08% were in the possible carcinogenic risk range (10−5 < LCR < 10−4 ), and 35.90% were in the negligible risk range (LCR < 10−6 ). The highest index of occupational carcinogenesis was observed in the group of lab technicians with a risk number of 3.7 × 10−4 , followed by pathologists with a risk number of 1.7 × 10−4 . Furthermore, 23.08% of the studied subjects were within the permitted health risk range (HQ < 1.0), while 76.92% were within the unhealthy risk range (HQ > 1.0). Overall, the findings revealed significantly higher carcinogenic and non-carcinogenic risks among lab technicians and pathologists. Therefore, it is imperative to implement control measures across various hospital departments to mitigate occupational formaldehyde exposure levels proactively. These findings can be valuable for policymakers in the health sector, aiding in the elimination or reduction of airborne formaldehyde exposure in work environments. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
45. Sericin promotes chondrogenic proliferation and differentiation via glycolysis and Smad2/3 TGF-β signaling inductions and alleviates inflammation in three-dimensional models.
- Author
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Fongsodsri, Kamonpan, Tiyasatkulkovit, Wacharaporn, Chaisri, Urai, Reamtong, Onrapak, Adisakwattana, Poom, Supasai, Suangsuda, Kanjanapruthipong, Tapanee, Sukphopetch, Passanesh, Aramwit, Pornanong, and Ampawong, Sumate
- Subjects
SERICIN ,THREE-dimensional modeling ,TRIOSE-phosphate isomerase ,GLYCOLYSIS ,ISOMERASES ,KNEE osteoarthritis - Abstract
Knee osteoarthritis is a chronic joint disease mainly characterized by cartilage degeneration. The treatment is challenging due to the lack of blood vessels and nerve supplies in cartilaginous tissue, causing a prominent limitation of regenerative capacity. Hence, we investigated the cellular promotional and anti-inflammatory effects of sericin, Bombyx mori-derived protein, on three-dimensional chondrogenic ATDC5 cell models. The results revealed that a high concentration of sericin promoted chondrogenic proliferation and differentiation and enhanced matrix production through the increment of glycosaminoglycans, COL2A1, COL X, and ALP expressions. SOX-9 and COL2A1 gene expressions were notably elevated in sericin treatment. The proteomic analysis demonstrated the upregulation of phosphoglycerate mutase 1 and triosephosphate isomerase, a glycolytic enzyme member, reflecting the proliferative enhancement of sericin. The differentiation capacity of sericin was indicated by the increased expressions of procollagen12a1, collagen10a1, rab1A, periostin, galectin-1, and collagen6a3 proteins. Sericin influenced the differentiation capacity via the TGF-β signaling pathway by upregulating Smad2 and Smad3 while downregulating Smad1, BMP2, and BMP4. Importantly, sericin exhibited an anti-inflammatory effect by reducing IL-1β, TNF-α, and MMP-1 expressions and accelerating COL2A1 production in the early inflammatory stage. In conclusion, sericin demonstrates potential in promoting chondrogenic proliferation and differentiation, enhancing cartilaginous matrix synthesis through glycolysis and TGF-β signaling pathways, and exhibiting anti-inflammatory properties. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
46. Exponential growth of private coastal infrastructure influenced by geography and race in South Carolina, USA.
- Author
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Beauvais, Jeffrey, Markley, Scott N., and Byers, James E.
- Subjects
PRIVATE property ,DEMOGRAPHIC characteristics ,AERIAL photography ,INCOME ,ENVIRONMENTAL justice ,ECOSYSTEMS - Abstract
Homeowners in coastal environments often augment their access to estuarine ecosystems by building private docks on their personal property. Despite the commonality of docks, particularly in the Southeastern United States, few works have investigated their historical development, their distribution across the landscape, or the environmental justice dimensions of this distribution. In this study, we used historic aerial photography to track the abundance and size of docks across six South Carolina counties from the 1950s to 2016. Across our roughly 60-year study period, dock abundance grew by two orders of magnitude, mean length of newly constructed docks doubled, and the cumulative length of docks ballooned from 34 to 560 km. Additionally, we drew on census data interpolated into consistent 2010 tract boundaries to analyze the racial and economic distribution of docks in 1994, 1999, 2011, and 2016. Racial composition, measured as the percentage of a tract's population that was White, positively correlated with dock abundance in each year. Median household income and dock abundance were only correlated in 2011. Taken together, these metrics indicate the growing desire for direct estuary access, however, that access does not appear to be equally spread across racial groups. Because docks enhance estuarine access and demarcate private property, our study provides longitudinal insights into environmental justice concerns related to disparate private property ownership. We found a persistent correlation between the racial characteristics of an area and dock abundance, strongly indicating that White South Carolinians have had disproportionately greater private water access for the past two decades. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
47. The importance of continents, oceans and plate tectonics for the evolution of complex life: implications for finding extraterrestrial civilizations.
- Author
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Stern, Robert J. and Gerya, Taras V.
- Subjects
PLATE tectonics ,DRAKE equation ,FERMI'S paradox ,OCEAN ,EXTRATERRESTRIAL beings - Abstract
Within the uncertainties of involved astronomical and biological parameters, the Drake Equation typically predicts that there should be many exoplanets in our galaxy hosting active, communicative civilizations (ACCs). These optimistic calculations are however not supported by evidence, which is often referred to as the Fermi Paradox. Here, we elaborate on this long-standing enigma by showing the importance of planetary tectonic style for biological evolution. We summarize growing evidence that a prolonged transition from Mesoproterozoic active single lid tectonics (1.6 to 1.0 Ga) to modern plate tectonics occurred in the Neoproterozoic Era (1.0 to 0.541 Ga), which dramatically accelerated emergence and evolution of complex species. We further suggest that both continents and oceans are required for ACCs because early evolution of simple life must happen in water but late evolution of advanced life capable of creating technology must happen on land. We resolve the Fermi Paradox (1) by adding two additional terms to the Drake Equation: f
oc (the fraction of habitable exoplanets with significant continents and oceans) and fpt (the fraction of habitable exoplanets with significant continents and oceans that have had plate tectonics operating for at least 0.5 Ga); and (2) by demonstrating that the product of foc and fpt is very small (< 0.00003–0.002). We propose that the lack of evidence for ACCs reflects the scarcity of long-lived plate tectonics and/or continents and oceans on exoplanets with primitive life. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
48. Exploring the utility of circulating miRNAs as diagnostic biomarkers of fasciolosis.
- Author
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Chowdhury, Sumaiya, Ricafrente, Alison, Cwiklinski, Krystyna, Sais, Dayna, Dalton, John P., Tran, Nham, and Donnelly, Sheila
- Subjects
FASCIOLIASIS ,MICRORNA ,GENE expression ,FASCIOLA hepatica ,PARASITIC diseases - Abstract
Effective management and control of parasitic infections on farms depends on their early detection. Traditional serological diagnostic methods for Fasciola hepatica infection in livestock are specific and sensitive, but currently the earliest detection of the parasite only occurs at approximately three weeks post-infection. At this timepoint, parasites have already entered the liver and caused the tissue damage and immunopathology that results in reduced body weight and loss in productivity. Here, we investigated whether the differential abundance of micro(mi)miRNAs in sera of F. hepatica-infected sheep has potential as a tool for the early diagnosis of infection. Using miRNA sequencing analysis, we discovered specific profiles of sheep miRNAs at both the pre-hepatic and hepatic infection phases in comparison to non-infected sheep. In addition, six F. hepatica-derived miRNAs were specifically identified in sera from infected sheep. Thus, a panel of differentially expressed miRNAs comprising four sheep (miR-3231-3p; miR133-5p; 3957-5p; 1197-3p) and two parasite miRNAs (miR-124-3p; miR-Novel-11-5p) were selected as potential biomarkers. The expression of these candidates in sera samples from longitudinal sheep infection studies collected between 7 days and 23 weeks was quantified using RT-qPCR and compared to samples from age-matched non-infected sheep. We identified oar-miR-133-5p and oar-miR-3957-5p as promising biomarkers of fasciolosis, detecting infection as early as 7 days. The differential expression of the other selected miRNAs was not sufficient to diagnose infection; however, our analysis found that the most abundant forms of fhe-miR-124-3p in sera were sequence variants (IsomiRs) of the canonical miRNA, highlighting the critical importance of primer design for accurate diagnostic RT-qPCR. Accordingly, this investigative study suggests that certain miRNAs are biomarkers of F. hepatica infection and validates miRNA-based diagnostics for the detection of fasciolosis in sheep. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
49. Experimental and theoretical aspects of the growth of vertically aligned CNTs by CCVD on AZO substrate.
- Author
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Nánai, Lilla, Németh, Zoltán, Kaptay, George, and Hernadi, Klara
- Subjects
CARBON nanotubes ,ZINC oxide ,CATALYST synthesis ,RAMAN spectroscopy ,CHEMICAL vapor deposition - Abstract
An efficient and reproducible growth of vertically aligned carbon nanotubes by CCVD requires accurate and specific setting of the synthesis parameters and the properties of catalyst thin layers. In this work, the growth of vertically aligned carbon nanotubes onto AZO (= aluminum doped zinc oxide) glass substrate covered by Al
2 O3 and Fe-Co catalyst layer system is presented. Investigation of the effect of catalyst composition and synthesis temperature on CVD growth revealed the optimum condition of the synthesis. The analysis of as-prepared samples by SEM, TEM and Raman spectroscopy was carried out to prove the structure and quality of carbon deposit. Theoretical considerations have supported speculative ideas about the role of the support layer, the transformation of the catalyst layer in the presence of hydrogen gas and the growth mechanism of carbon nanotubes. The mechanism of CNT growth is modelled and the order of magnitude of experimentally observed vertical linear growth rate of CNT (several nm/s) is reproduced. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
50. Dynamic analysis of geomaterials using microwave sensing.
- Author
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Blanche, Jamie, Mitchell, Daniel, Shang, Junlong, Flynn, David, Pavuluri, Sumanth, and Desmulliez, Marc
- Subjects
ENERGY security ,ENERGY storage ,PETROPHYSICS ,RADAR ,MICROWAVES ,SENSES - Abstract
Precise characterization of geomaterials improves subsurface energy extraction and storage. Understanding geomaterial property, and the complexities between petrophysics and geomechanics, plays a key role in maintaining energy security and the transition to a net zero global carbon economy. Multiple sectors demand accurate and rapid characterization of geomaterial conditions, requiring the extraction of core plugs in the field for full-field characterization and analysis in the laboratory. We present a novel technique for the non-invasive characterization of geomaterials by using Frequency Modulated Continuous Wave (FMCW) radar in the K-band, representing a new application of microwave radar. We collect data through the delivery of FMCW wave interactions with geomaterials under static and dynamic conditions and show that FMCW can detect fluid presence, differentiate fluid type, indicate the presence of metallic inclusions and detect imminent failure in loaded sandstones by up to 15 s, allowing for greater control in loading up to a failure event. Such precursors have the potential to significantly enhance our understanding of, and ability to model, geomaterial dynamics. This low-cost sensing method is easily deployable, provides quicker and more accessible data than many state-of-the-art systems, and new insights into geomaterial behavior under dynamic conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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