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Start Over Topic amino acyl-trna synthetases Journal scientific reports
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1. Development of a one-step analysis method for several amino acids using a microfluidic paper-based analytical device.

Author

Kugimiya A, Wakimoto S, Kohda J, Nakano Y, and Takano Y

Subjects
Amino Acids, Histidine analysis, Lab-On-A-Chip Devices, Microfluidics, Paper, Amino Acyl-tRNA Synthetases chemistry, Microfluidic Analytical Techniques
Abstract

A one-step analysis method was developed for four types of amino acids using a microfluidic paper-based analytical device fabricated from chromatography filtration paper and laminate films. Aminoacyl-tRNA synthetase was used to detect each amino acid. The obtained laminated paper-based analytical device (LPAD) contained four enzymatic reaction areas. Colorimetric detection was performed based on the molybdenum blue reaction. A model method for the simple, easy, and simultaneous detection of several amino acid concentrations was suggested, in contrast to the conventional methods such as HPLC or LC-MS. The method provided a selective quantification at the ranges of 3.6-100 μM for tryptophan, 10.1-100 μM for glycine, 5.9-100 μM for histidine and 5.6-100 μM for lysine with a detection limit of 1.1 μM, 3.3 μM, 1.9 μM and 1.8 μM, respectively. LPAD fabrication was considerably simple, and the subsequent detection process was easy and required a short period of time (within 15 min)., (© 2022. The Author(s).)

Published
2022
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2. Development of a one-step analysis method for several amino acids using a microfluidic paper-based analytical device

Author

Akimitsu, Kugimiya, Sho, Wakimoto, Jiro, Kohda, Yasuhisa, Nakano, and Yu, Takano

Subjects
Amino Acyl-tRNA Synthetases, Paper, Multidisciplinary, Lab-On-A-Chip Devices, Microfluidics, Histidine, Amino Acids, Microfluidic Analytical Techniques
Abstract

A one-step analysis method was developed for four types of amino acids using a microfluidic paper-based analytical device fabricated from chromatography filtration paper and laminate films. Aminoacyl-tRNA synthetase was used to detect each amino acid. The obtained laminated paper-based analytical device (LPAD) contained four enzymatic reaction areas. Colorimetric detection was performed based on the molybdenum blue reaction. A model method for the simple, easy, and simultaneous detection of several amino acid concentrations was suggested, in contrast to the conventional methods such as HPLC or LC–MS. The method provided a selective quantification at the ranges of 3.6–100 μM for tryptophan, 10.1–100 μM for glycine, 5.9–100 μM for histidine and 5.6–100 μM for lysine with a detection limit of 1.1 μM, 3.3 μM, 1.9 μM and 1.8 μM, respectively. LPAD fabrication was considerably simple, and the subsequent detection process was easy and required a short period of time (within 15 min).

Published
2022