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7 figures, 4 tables.-- Supplementary information available.-- © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/, Developing chrome-free and sustainable tanning agents is extremely important to the sustainability of the leather industry. Herein, we have synthesized an Al-Zr-oligosaccharides tanning agent via a simultaneous degradation and oxidation of cellulose in waste paper. The influence of the temperature and the concentrations of AlCl3 and H2O2 during the synthesis were thoroughly investigated on the properties of the tanning agent and the leather produced. The synthesis temperature and the concentration of AlCl3 were the factors primarily affecting the effective depolymerization of cellulose. They controlled the conversion of waste paper into oligosaccharides with an appropriate molecular weight to efficiently penetrate the leather matrix. In parallel, the H2O2 concentration substantially influenced the tanning performance of the Al-Zr-oligosaccharides, diminishing the chromaticity of the tanning liquid via oxidation and promoting the conversion of C2/C3/C6-OH moieties into -CHO/-COOH. These functional groups increased the surface charge of the oligosaccharides allowing more effective coordination with Al/Zr, which facilitated the penetration of Al/Zr species into the leather matrix. Once inside the leather matrix, Al and Zr were released and reacted with the collagen fibers in leather, which resulted in effective leather tanning. The process optimization revealed that up to 57% of waste paper could be converted into a low-chromaticity (4350 AU) liquid hydrolysate with the synthesis conducted at 177 °C in a system comprising 47 mM AlCl3 and 5 vol% H2O2. The application of this liquid for tanning provided leather with a shrinkage temperature (86.5 °C) sufficiently high for commercial applications. These excellent results, combined with the intrinsic green nature of our approach, exemplify a step forward to simultaneously reduce pollution and hazards in leather industries giving a second life to waste paper., This work is financially supported by the National Natural Science Foundation of China (22078211). Javier Remón acknowledges the Spanish Ministry of Science, Innovation and Universities for the Juan de la Cierva (JdC) fellowship (Grant Number: IJC2018-037110-I) awarded.

Currently, petroleum-based synthetic plastics are used as a key barrier material in the paper-based packaging of several food and nonfood goods. This widespread usage of plastic as a barrier lining is not only harmful to human and marine health, but it is also polluting the ecosystem. Researchers and food manufacturers are focused on biobased alternatives because of its numerous advantages, including biodegradability, biocompatibility, non-toxicity, and structural flexibility. When used alone or in composites/multilayers, these biobased alternatives provide strong barrier qualities against grease, oxygen, microbes, air, and water. According to the most recent literature reports, biobased polymers for barrier coatings are having difficulty breaking into the business. Technological breakthroughs in the field of bioplastic production and application are rapidly evolving, proffering new options for academics and industry to collaborate and develop sustainable packaging solutions. Existing techniques, such as multilayer coating of nanocomposites, can be improved further by designing them in a more systematic manner to attain the best barrier qualities. Modified nanocellulose, lignin nanoparticles, and bio-polyester are among the most promising future candidates for nanocomposite-based packaging films with high barrier qualities. In this review, the state-of-art and research advancements made in biobased polymeric alternatives such as paper and board barrier coating are summarized. Finally, the existing limitations and potential future development prospects for these biobased polymers as barrier materials are reviewed.

Phosphate adsorption using metal-based biochar has awakened much attention and triggered extensive research. In this study, novel Ca/Fe-rich biochars were prepared via a one-step process of pyrolyzing paper mill sludge (PMS) at various temperatures (300, 500, 700, and 800 °C) under a CO

The synergistic effect of heteroatoms is a viable method to enhance the adsorption performance of heavy metal onto carbon-based materials. However, the high cost, complex operation and a lot of pollution from the synthesis process have limited its development. Herein, a facile two-step pyrolysis method is used to prepare in situ N and S doped porous biochar from paper mill sludge for the removal of Cr(VI) from aqueous environment. The NSC-450 sample prepared under the optimum conditions has a large specific surface area of 3336.7 m

This paper was written to comment on a few important problems of an original paper published in this journal. In the original paper, polyamide (PA) ropes, a kind of plastic, were used for groundwater sampling. Also, polycarbonate, another plastic, was also used as a filter paper although their potential contamination was later evaluated. Although the original authors reported that high levels of PA were not found in any of the 21 groundwater samples, it is still necessary to only use equipment(s) made of non-plastic at every step of the method for an accurate and reliable analysis of the presence of microplastic in groundwater. The original authors collected a total of 3 l for each borehole (1 l for each sample), but for an unbiased and reliable analysis of microplastics, bigger volumes of groundwater samples should be collected. Furthermore, the original authors computed the Pearson correlation coefficients between the analyzed plastic types, but omitted the normality test of the data distribution. If the collected data are not normally distributed, then Spearman rank correlation coefficients are a better option. In addition, we found some important misstatements regarding the results of the analysis.

Risk assessments of agrochemicals on non-target insects are biased in studies with surrogate groups, such as pollinators. In social insects, such investigations are generally restricted to lethal tests with adults maintained individually, simulating a non-realistic scenario. Here, we performed a holistic approach to resemble a chronic field exposure of Polistes versicolor (Hymenoptera: Vespidae) to a common biopesticide. These wasps are predators that perform biological control in the agroecosystems. Wasps were chronically subjected to the ingestion of different concentrations of azadirachtin. The neonicotinoid imidacloprid was used as a positive control. For the first time, we demonstrated that the biopesticide azadirachtin is detrimental for individual and colony survival and impairs colony reproduction of a social wasp maintained in the laboratory. Our data also indicated that neonicotinoid imidacloprid is harmful to wasps and their colonies. Therefore, the concomitant use of azadirachtin and paper wasps in integrated pest management strategies should be carefully evaluated, because the constant use of this pesticide can be detrimental for social wasps, possibly reducing biological control.

Currently, the performance evaluation of catalysts usually requires expensive instruments. Hence, it is imperative to develop an alternative, green and sustainable method to investigate the photocatalytic reaction processes. Herein, the variation of degradation performance of different wastewaters with different dosage of P25 TiO2 was evaluated to verify the reliability of the paper microzones method (PMZs). The optimum P25 TiO2 dosage of 1 g/L for the degradation of methylene blue (MB) (UV light for 6 mins) and 0.5 g/L for the degradation of fuchsin basic (FB) (UV light for 5 mins) was obtained by the PMZs method. For the photocatalytic degradation of trivalent iron ion complexed salicylic acid (Fe(III)-SA) solution, the R2 values of 0.904 and 0.801 were obtained for the photocatalytic reaction kinetics by PMZs and spectrophotometry, respectively, which again indicated the high reliability of PMZs. The accuracy of the results obtained by PMZs method relative to the spectrophotometric method ranged from 68.80% to 87.54% when degrading MB, FB, mixture of MB and FB, and Fe(III)-SA by P25 TiO2. Therefore, the PMZs method is all in line with the requirements of low-carbon environmental protection and green chemistry, and has broad application prospects in the future.

Let's imagine that you have just finished writing a scientific paper. The paper is well-structured and clearly written, and you are proud of it. Now is the time to submit it to a peer-reviewed journal and see what your colleagues think of it. You are now entering the peer-review publishing system, which is overseen by journal editors. Dealing with these editors is a skill that can be acquired like any other. Here is some advice on dealing with the peer-review system and with editors. This advice is based on my years of experience as an associate editor of an American Chemical Society journal. I have also submitted and revised hundreds of papers in my career and have reviewed hundreds more. (Google my name for details.) Thus, I have learned how to deal with editors from both sides.

In the present study we evaluated cytotoxic and genotoxic activities of endocrine disrupting compounds (EDCs), including dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), bisphenol A (BPA), and nonylphenol (NP), which have been previously identified in effluents from two paper mills with different paper production technologies (virgin or recycled fibres). Moreover, we evaluated genotoxic activity of the effluents from these two paper mills and compared it to the activity of artificial complex mixtures consisting of the seven EDCs at concentrations detected in corresponding paper mill effluents. None of the EDCs was genotoxic in Salmonella typhimurium (SOS/umuC assay), while all induced DNA damage in human hepatocellular carcinoma (HepG2) cells (comet assay). After 4 h of exposure genotoxic effects were determined at concentrations ≥ 1 μg/L for BBP and DEHP, ≥10 μg/L for DMP, DEP, DBP, and BPA, and ≥100 μg/L for NP, while after 24 h of exposure DNA damage occurred at ≥10 μg/L for DBP, BPA and NP, and ≥100 μg/L for DMP, DEP, BBP and DEHP. The effluents and corresponding artificial mixtures of EDCs from paper mill that uses virgin fibres did not induce DNA damage in HepG2 cells, while the effluents and corresponding artificial mixtures for the paper mill that uses recycled fibres were genotoxic. Genotoxic activity of effluents was significantly higher compared to corresponding artificial mixtures suggesting the presence of further unknown compounds contributing to the effect. Wastewater monitoring based on chemical analysis is limited to determination of targeted compounds and does not take into account possible interactions between chemicals in mixtures. Therefore, it alone cannot provide an adequate information on potential toxic effects required for the assessment of genotoxic activity of real environmental samples and their potential threats to the environment and human health.

Different types of pots and growing and casing media, including biodegradable materials, are used for plant and mushroom production. The fungus Peziza ostracoderma has gained attention for its visible growth on growing media for plants and casing media for mushrooms. Through a review of the literature we aim to evaluate whether exposure to fungi from recyclable pots and different growing and casing media occurs and causes occupational health effects. Based on the published papers, specific fungal species were not related to a specific medium. Thus P. ostracoderma has been found on paper pots, peat, sterilized soil, vermiculite, and rockwool with plants, and on peat, pumice, and paper casing for mushrooms. It has been found in high concentrations in the air in mushroom farms. Also Acremonium spp., Aspergillus niger, A. fumigatus, Athelia turficola, Aureobasidium pullulans, Chaetomium globosum, Chrysonilia sitophila, Cladosporium spp., Cryptostroma corticale, Lecanicillium aphanocladii, Sporothrix schenckii, Stachybotrys chartarum, and Trichoderma spp. have been found on different types of growing or casing media. Most of the fungi have also been found in the air in greenhouses, but the knowledge about airborne fungal species in mushroom farms is very limited. Eight publications describe cases of health effects associated directly with exposure to fungi from pots or growing or casing media. These include cases of hypersensitivity pneumonitis caused by exposure to: A. fumigatus, A. niger, Au. pullulans, Cr. corticale, P. ostracoderma, and a mixture of fungi growing on different media. Different approaches have been used to avoid growth of saprophytes including: chemical fungicides, the formulation of biodegradable pots and growing media and types of peat. To increase the sustainability of growing media different types of media are tested for their use and with the present study we highlight the importance of also considering the occupational health of the growers who may be exposed to fungi from the media and pots.

The contribution of improperly disposed plastic wastes is globally evaluated at the level of 30% and these wastes make a particular threat to all living creatures. Thus, the evaluation of the possible impacts of plastic particles on the biotic part of ecosystems has become increasingly important in recent years. As a result, the growing number of publications concerning this subject has been observed since 2018. This paper aims to review the advances in studies on the effect of petroleum-derived plastic and bioplastic particles, taken together in the term (bio)plastics, on the terrestrial ecosystem, particularly on soil biota. It is the first review, in which both petroleum-derived plastics and bioplastics were analysed regarding their potential impacts on the soil compartment. Petroleum-derived plastics were more frequently studied than bioplastics and among analysed papers about 18% concern bioplastics. It was found that (bio)plastics did not affect the germination of seeds. However, they might contribute to the delay in germination processes. Both inhibitory and stimulating effects were observed in relation to the growth of roots and stems. (Bio)plastic microparticles did not inhibit the biochemical activity of nitrifiers and transformation of carbon compounds. Earthworms were predominantly used organisms to test the effect of petroleum-derived plastics on soil biota but there are hardly any data about bioplastics. Petroleum-derived microplastics present in soil at concentrations up to 1000 mg kg-1 usually neither cause to the mortality of earthworms nor affect their reproduction. Micro- and nanoparticles of petroleum-derived plastics could be accumulated in the earthworm intestine and transferred in the food chain. Summarizing, a high variability of results and often appearing lack of dose-dependence relationships hamper the final evaluation of the ecotoxicity of (bio)plastics simultaneously creating a need to develop the ecotoxicological studies on (bio)plastics, especially including these on the effect of bioplastics on soil animals.

Macrophytes have been widely used as agents in wastewater treatment. The involvement of plants in wastewater treatment cannot be separated from wetland utilization. As one of the green technologies in wastewater treatment plants, wetland exhibits a great performance, especially in removing nutrients from wastewater before the final discharge. It involves the use of plants and consequently produces plant biomasses as treatment byproducts. The produced plant biomasses can be utilized or converted into several valuable compounds, but related information is still limited and scattered. This review summarizes wastewater's nutrient content (macro and micronutrient) that can support plant growth and the performance of constructed wetland (CW) in performing nutrient uptake by using macrophytes as treatment agents. This paper further discusses the potential of the utilization of the produced plant biomasses as bioenergy production materials, including bioethanol, biohydrogen, biogas, and biodiesel. This paper also highlights the conversion of plant biomasses into animal feed, biochar, adsorbent, and fertilizer, which may support clean production and circular economy efforts. The presented review aims to emphasize and explore the utilization of plant biomasses and their conversion into valuable products, which may solve problems related to plant biomass handling during the adoption of CW in wastewater treatment plants.

The widespread deterioration of our water systems requires new wastewater treatment technologies to ensure environmental protection. Conventional wastewater treatments were not designed for, and are therefore ineffective, at removing contaminants of emerging concern (CECs) such as pharmaceuticals, personal care products, pesticides, and industrial chemicals. Furthermore, treatment processes capable of breaking down CECs may produce toxic transformation products more harmful than the parent chemicals. Heterogeneous photocatalytic ozonation provides a promising option with high degradation and mineralization of organic compounds. The aim of the present paper is to review ecotoxicity reduction in water treated by heterogeneous photocatalytic ozonation as a measure of process viability. The discussion investigates changes in toxicity based on a variety of toxicity tests performed to evaluate potential effects on ecosystems, the types of catalysts and radiation sources used, the nature of the target contaminants, and the type of water matrix treated. Acute toxicity testing, TiO2 catalysts, and mercury-vapour lamps including blacklights were dominant in the reviewed studies, investigated in 86%, 84% and 79% of the papers, respectively. Pharmaceuticals were the main group of chemicals treated and the water matrices used were predominantly pure water and secondary effluent. Overall, the findings of these studies provide evidence that photocatalytic ozonation is an efficient process to remove persistent organic compounds while, most of the time, not increasing the toxicity of the effluent (as reported by 86% of the studies). Due to the wide variation in experimental set-ups, no clear correlation between reaction conditions and toxicity was determined, however, V. fischeri acute toxicity assays and chronic/sublethal tests appeared most sensitive to transformation products. Future studies need to a) incorporate multiple toxicity tests to produce a more reliable and inclusive ecotoxicity assessment of treated effluent and b) investigate immobilized catalysts and energy efficient radiation sources (i.e. solar and LEDs) for industrial applications.

Antibiotic resistance is considered a universal health threat of the 21st century which its distribution and even development are mainly mediated by water-based media. Disinfection processes with the conventional methods are still the most promising options to combat such crises in aqueous matrices especially wastewater. Knowing that the extent of effectiveness and quality of disinfection is of great importance, this paper aimed to systematically review and discuss ozonation (as one of the main disinfectants with large scale application) effect on removing antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) from aqueous solutions, for which no study has been reported. For this, a comprehensive literature survey was performed within the international databases using appropriate keywords which yielded several studies involving different aspects and the effectiveness extent of ozonation on ARB & ARGs. The results showed that no definite conclusion could be drawn about the superiority of ozone alone or in a hybrid form. Mechanism of action was carefully evaluated and discussed although it is still poorly understood. Evaluation of the studies from denaturation and repairment perspectives showed that regrowth cannot be avoided after ozonation, especially for some ARB & ARGs variants. In addition, the comparison of the effectiveness on ARB & ARGs showed that ozonation is more effective for resistant bacteria than their respective genes. The degradation efficiency was found to be mainly influenced by operational parameters of CT (i.e. ozone dose & contact time), solids, alkalinity, pH, and type of pathogens and genes. Moreover, the correlation between ARB & ARGs removal and stressors (such as antibiotic residuals, heavy metals, aromatic matters, microcystins, opportunistic pathogens, etc.) has been reviewed to give the optimal references for further in-depth studies. The future perspectives have also been reported.

Microplastics (MPs) are the emerging pollutants in the fresh water and have been found in the drinking water. Drinking water treatment plant (DWTP) is an important barrier to ensure the safety and quality of drinking water, so their effectiveness in removing MPs needs to be evaluated and optimized. In this paper, the abundance, characteristics and removal performance of 5 μm-5 mm granular MPs and 100 μm-5 mm fibrous MPs in the effluent of each water treatment unit in a DWTP in China were analyzed. The results show that only 80.96% of MPs ≤ 20 μm, accounting for more than 98% in the raw water, could be removed in the DWTP, while over 99% of the removal efficiency could be achieved for MPs > 20 μm. Coagulation-sedimentation and micro-flocculation combined with sand filtration couldn't effectively remove the granular MPs ≤ 20 μm (42.8% and 25.8%, respectively), but biological activated carbon (BAC) filtration was prone to remove this part (63.8%). Significant increases of granular MPs ≤ 20 μm were observed in the effluents of biological treatment (+149.6%) and ozonation (+13.8%). According to the variation in separate MPs' size distribution, the increases in MPs were attributed to the fragmentation of MPs, on which ozonation showed a remarkable effect. The ozonation might accelerate the aging and embrittlement of MPs, and then external stresses could further break them into smaller pieces, which eventually lead to an increase in MPs. To deal with the stubborn part and fragmentation of MPs in water, the removal ability of DWTP for MPs ≤ 20 μm needs to be improved. This study provides a detailed proof for the fate of MPs in the DWTP, and the results indicate that the fragmentation of MPs and the removal rate of MPs ≤ 20 μm should draw more attention in the DWTP.

As cost-effective and high-efficient oxidants, the hypochlorite chemicals have been widely utilized for bleaching and disinfection. However, its potential applications in wastewater treatment and sludge disposal were less concerned. This paper mainly summarized the state-of-the-art applications of hypochlorite technology in wastewater and sludge treatment based on the main influencing factors and potential mechanisms of hypochlorite treatment. The results indicated that the hypochlorite approaches were not only effective in pollutants removal and membrane fouling mitigation for wastewater treatment, but also contributed to sludge dewatering and resource recovery for sludge disposal. The ClO- and large generated free active radicals (i.e., reactive chlorine species and reactive oxygen species), which possessed strong oxidative ability, were the primary contributors to the pollutants decomposition, and colloids/microbes flocs disintegration during the hypochlorite treatment process. The performance of hypochlorite treatment was highly associated with various factors (i.e., pH, temperature, hypochlorite types and dosage). In combination with the reasonable activators (i.e., Fe2+ and ultraviolet), auxiliary agents, and innovative processes (i.e., hydrothermal and electro-oxidation), the operational performance of hypochlorite technology could be further enhanced. Finally, the feasibility and benefits of hypochlorite application for wastewater and sludge treatment were analyzed, and the existing challenges and future research efforts that need to be made have also prospected. The review can hopefully provide a theoretical basis and technical guidance to extend the application of hypochlorite technology for wastewater treatment and sludge disposal on large scale.

This paper presents an eco-efficiency analysis of five scenarios for treating a petrochemical unit effluent defined sequentially to increase the water reuse rate to the limit condition of Zero Liquid Discharge perspective. The base process, consisting of reverse osmosis, an evaporator and crystallizer, displayed pretreatment alternatives the addition of antifouling agents, desupersaturation of barium ions and coprecipitation of low solubility salts to increase osmosis efficiency. The eco-efficiency analysis applied the life cycle approach to verify environmental and energy performances and determined the costs of the treatment unit installation and operation for a time horizon of 9.5 years. The pretreatment increased water recovery during osmosis from 84% to over 97%. The environmental-energetic analysis indicated a strong influence of electric energy, consumed mainly during the thermal stages of the treatment, and of the chlorine-derived compounds used in the pretreatment. Also due to pre-treatment, it was possible to reduce impacts in terms of Global Warming Potential by up to 59%, and in Primary Energy Demand by 64% for the best scenario. The economic analysis depicted a significant influence of operating costs, and the electricity consumed in the processes is responsible for the largest share of expenses. The reuse water presented a cost ranging between US$ 1.08/m3 and US$ 1.80/m3. The systems with pretreatment by coprecipitation with Ca(OH)2 and CaCO3 and monoacids to adjust the pH of the final effluent showed to be more eco-efficient than the other options under analysis.

Although numerous studies have detected SARS-CoV-2 RNA in wastewater and attempted to find correlations between the concentration of SARS-CoV-2 RNA and the number of cases, no consensus has been reached on sample collection and processing, and data analysis. Moreover, the fate of SARS-CoV-2 in wastewater treatment plants is another issue, specifically regarding the discharge of the virus into environmental settings and the water cycle. The current study monitored SARS-CoV-2 RNA in influent and effluent wastewater samples with three different concentration methods and sludge samples over six months (July to December 2020) to compare different virus concentration methods, assess the fate of SARS-CoV-2 RNA in wastewater treatment plants, and describe the potential relationship between SARS-CoV-2 RNA concentrations in influent and infection dynamics. Skimmed milk flocculation (SMF) resulted in 15.27 ± 3.32% recovery of an internal positive control, Armored RNA, and a high positivity rate of SARS-CoV-2 RNA in stored wastewater samples compared to ultrafiltration methods employing a prefiltration step to eliminate solids in fresh wastewater samples. Our results suggested that SARS-CoV-2 RNA may predominate in solids, and therefore, concentration methods focusing on both supernatant and solid fractions may result in better recovery. SARS-CoV-2 RNA was detected in influent and primary sludge samples but not in secondary and final effluent samples, indicating a significant reduction during primary and secondary treatments. SARS-CoV-2 RNA was first detected in influent on September 30th, 2020. A decay-rate formula was applied to estimate initial concentrations of late-processed samples with SMF. A model based on shedding rate and new cases was applied to estimate SARS-CoV-2 RNA concentrations and the number of active shedders. Inferred sensitivity of observed and modeled concentrations to the fluctuations in new cases and test-positivity rates indicated a potential contribution of newly infected individuals to SARS-CoV-2 RNA loads in wastewater.

The oxygen based membrane biofilm (O2-MBfR) has been proved to be a novel technology in treating greywater (GW) and response surface methodology (RSM) was used to model the removal of chemical oxygen demand (COD) and total nitrogen (TN) with operation parameters COD/TN ratio, system pH and lumen air pressure (LAP). Results indicated that the all target single factors affect GW treatment efficiency, and the regression model with central composite design (CCD) showed good agreement with the experimental results with high R2 and R2 adj values (all >0.97) for all the target responses. Statistical evaluation revealed that system pH was the most significant parameter affecting COD and TN removal, followed by COD/TN ratio and LAP. The interaction between COD/TN ratio and system pH also played an important role on the GW treatment. The optimized maximum removal of COD (96.48%) and TN (133 g N/m2-day) were achieved with the COD/TN ratio 17.76 g COD/g TN, system pH 7.10 and LAP 1.00 psi. Thus, RSM combined with CCD could be used for predicting the organics and nitrogen removal during GW treatment in the O2-MBfR.

Hydraulic fracturing wastewater (HFW), a byproduct of hydraulic fracturing oil extraction, contains a complex mixture of oil, aldehydes, and benzene compounds. Efficient and eco-friendly HFW treatment means are critical for the oil extraction industry, particularly in developing countries. In this study, two biological processes namely an anaerobic/anoxic/moving bed biofilm reactor (A2-MBBR) and an A2-MBBR with a microfiltration membrane (A2-MFMBBR) were established, and assessed for the real HFW treatment. Removal efficiencies of chemical oxygen demand (COD) and NH4+-N were over 92% and 95%, respectively, in both processes with a hydraulic retention time of 72 h. The majority of organic compounds in both systems identified by GC–MS were degraded in the anaerobic units. In comparison, A2-MFMBBR demonstrated higher removal efficiencies for oil, total suspended solids, and complex compounds. The average relative abundances of refractory compound degrading bacteria were 43.4% and 51.6% in the A2-MBBR and A2-MFMBBR, respectively, which was consistent with the COD and oil removal, and suggested that the MBR could maintain a high diversity of microorganisms and contribute to deep recalcitrant organics degradation. This study sheds light on the potential of using a compact biological process for the real HFW treatment.