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Staphylococcus aureusis a common human bacterium that sometimes becomes pathogenic, causing serious infections. A key feature of S. aureusis its ability to acquire resistance to antibiotics. The presence of the staphylococcal cassette chromosome (SCC) element in serotypes of S. aureushas been confirmed using multiplex PCR assays. The SCC element is the only vector known to carry the mecAgene, which encodes methicillin resistance in S. aureusinfections. Here, we report the genome sequence of a novel methicillin-sensitive S. aureus(MSSA) strain: SCC-like MSSA463. This strain was originally erroneously serotyped as methicillin-resistant S. aureusin a clinical laboratory using multiplex PCR methods. We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S. aureusisolates. An open reading frame (CZ049; AB037671) was identified downstream of attLand attRinverted repeat sequences. Our results suggest that a lateral gene transfer occurred between S. aureusand other organisms, partially changing S. aureusinfectivity. We propose that attLand attRinverted repeats in S. aureusserve as frequent insertion sites for exogenous genes.

Using a pyrosequencing-based custom-made sequencer BIGIS-4, we sequenced a Gram-negative bacterium Glaciecola mesophilasp. nov. (Gmn) isolated from marine invertebrate specimens. We generated 152043 sequencing reads with a mean high-quality length of 406 bp, and assembled them using the BIGIS-4 post-processing module. No systematic low-quality data was detected beyond expected homopolymer-derived errors. The assembled Gmngenome is 5144318 bp in length and harbors 4303 annotated genes. A large number of metabolic genes correspond to various nutrients from surface marine invertebrates. Its abundant cold-tolerant and cellular signaling and related genes reveal a fundamental adaptation to low-temperature marine environment.